CHO et al. Supplementary Figure 1

*

**

Supplementary Fig. 1. Primary human epidermal keratinocytes were grown in dermal cell

culture medium with growth supplements described in Materials and Methods. Primary

keratinocytes were treated with 100 ng/ml of either IL-17, IL-22, or IL-1, for 24 hrs, and

culture supernatants were collected for the measurement of active form of IL-1 by ELISA.

Data are expressed as the means SEM of three independent experiments and compared

with those of the ‘no treatment’ group (P < 0.05).

CHO et al. Supplementary Figure 2

***

Gr-1

No Tx IL-17 IL-22 IL-1β

C56BL/6

Caspase-1 KO

CD

11b

6.7 47 31 36

1.8 5.3 234.2

CD

11c

C56BL/6

Caspase-1 KO

B

No Tx IL-17 IL-22 IL-1β

3.8 21 9 15

2.8 4 3.2 7

A

C

Class II MHC

Supplementary Fig. 2. The total cell numbers and the proportion of neutrophils (Gr-1+ ,

CD11b+ cells) and dendritic cells (CD11c+, class II MHC+ cells) in WT and caspase-1 KO

mice. (A) Total cells isolated from mice ears were collected and numbered. Data are

expressed as means ± SEM of three independent experiments (* P < 0.05). (B) Cells

isolated from ears of mice were stained for 20 min at room temperature with anti-mouse

Ly-6G/Ly-6C (Gr-1) (108405, BioLegend), anti-CD11b (101207, BioLegend) to detect

neutrophils. (C) Cells were stained with anti-mouse CD11c (117307, BioLegend, San

Diego, CA, USA), and anti-mouse I-A/I-E (107605, BioLegend) to detect dendritic cells.

Data were acquired on a FACSCalibur system (BD Bioscience) and analyzed using

CellQuest software (BD Bioscience).

CHO et al. Supplementary Figure 1