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Chromatography 研習資料. Separation of Pigments In a solvent according To their densities. Separate plant pigments. Also for Forensics. Find type of ink (different color separations) Find impurities in products. Taper Strip. Cut to a point with scissors. Mark a starting line. Use pencil - PowerPoint PPT Presentation
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Chromatography研習資料
Separation of Pigments
In a solvent according
To their densities
Separate plant pigments
Also for Forensics
• Find type of ink
(different color separations)
• Find impurities in products
Taper Strip
• Cut to a point with scissors
Mark a starting line
• Use pencil
• (not ink)
Chemical Application
Add small drops, let dry, add again until dark spot
KEEP ABOVE PENCIL LINE
Use coin (mechanical) – geranium leaf
• Use serrated coin
• Do a few times to make a dark line
Be sure liquid is below line
• Attach paper to paper clip
• Attach paper clip to stopper
Chromatography Animation
Paper Chromatography Videos
Chromatography Over Time
• Time Lapse Video• (Be patient and give it
time to move)
Mark bands of colorsLighter pigments will move farther up the paper
Heavier pigments will remain near the start
Pencil line will mark start point
Separation of Ink
Calculating Rf
• Compares distance the solvent traveled to the distance the test sample traveled.
• Rf = distance of test sample
• distance solvent
Measure tops of colors
• From starting point at bottom to top of each color
Distance
of solvent
Pigments in Plants
• carotene-yellow orange
• chlorophyll a-bright green
• Xanthophylls-yellow
• chlorophyll b-khaki green
More colors
• phycocyanin = bluish• phycoerythrin = reddish
Carrier Solution
• Stored in da hood!
• Return the waste to the waste container in da hood.
• Boy-e-e-e-e-e-e-e-e-e-e!
Sample Results
Click Here for Examples
• Results of Chromatograph
Accessory Pigments
• fucoxanthin = brown
• Phycobilin = reds
• phycocyanin = bluish
Conclusion Question #2