Clostrodium Botulinum

7/30/2019 Clostrodium Botulinum

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Company name

Company

LOGO

GVHD:ThS oHng HSVTH: Nhm 3 3


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Company name

Clostridium botulinum l g?

C.botulinum l vi khun Gram+ chai u trn, thng hin din trongmi sinh, t ct, rau qu, tht v c.Khi iu kin pht trin tr nn khkhn, vk s chuyn sang dng bo t

c th chu ng c nhit cao, c bit l trong mi trng cdu... C. botulinum pht trin ddng trong iu kin m v khngoxy hay ym kh hon ton. Trong

lc tng trng, bo t sn sinh ramt loi c t cc mnh. Ngi tangh rng ch cn mt mung cfc t thi cng sc git cht100.000 ngi.


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Company name

Nidung chnh

1. Tng quan v Clostridium botulinum.2. cimca vi sinh vt clostridium

botulinum.3. Bnh sinh v cc tnh nng lm sng.4. Phn lp v nhndng.

5. Hiphithcphm.KT LUN


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Company name

1. Tngquanv Clostridium botulinum.


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Company name

1.1. Lchsphthinkhun Clostridiumbotulinum

Xuthinlnutin vo nm1793 Wildbad, Wurttemburg trong mtloi xcxch.

Nm 1986 Van Ermengem GS vi trnghcgiy l vi khunbotulinus.

V sau cn pht hin trong c thi, utrngnghp.


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Company name

2. cimca vi sinhvtclostridium botulinum

cim hnh thi

cimcu trc


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Company name

2.1. Phnloi.

C 6 loictc xc nh, phn btA n G.

Loi A, B, E: ngc nguy him, tc ngln hthngthn kinh, gy chtngi.

Loi A, B, E, F: gy bnh ngi; C, D:gy bnh b, chim v cc ngvt khc

Loi A thngthy chu M, loiB

chu u, loiE NhtBn.


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Company name

Nhm I Nhm II Nhm III Nhm IVCc loict A, B, F B, E, F C1,C2, D GPhn gii Protein C Khng Khng CPhn gii Lipid C C C KhngPhn gii Saccharose C C C Khng

Psychrotrophic

(t0mintngtrng)Khng

(10120C)C

(3-50C)Khng

(150C) (120C)Scchcamui

(aw)10%

(0.94)5%

(0.075)3% > 3%

itng gy bnh Con ngi Con ngi ngvt v chim Con ngi

ctgen Nhimscth Nhimscth bacteriophage plasmidClose relative C.sporogennes

C. putrificumC. butyricum

C.beijerinickiiC.haemolyticum

C.novy type AC.subterminale

C.haemolyticumBnnhit C

(cht 1210C, 0.1-

0.25 pht)

Khng

(cht 800C, 0.6-3.3

pht)

2.2.Phn nhm..


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Company name

- L cc vi khunGram dng,hnh que.

- Sngk khbtbuc.

- Sinh bo t.

- Phnln l ding.

- C ththy phnng v Protein.

- Mtsanhit,mtsalnh

- Cc loi gy ngc quan trng:C.botulinum,C.perfringens

2.3. cim chung..


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Company name

2.3.1. cim hnh thi.

pH tia

Khongt8.5 8.9 Cc chtc khng bn pH ny

pH tithiuPhthucnhiu vo t0, aw, acid

iuchnh pH Gi tr vo khong 4.7

pH


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Company name

2.3.1. cim hnh thi

- Nhit 30 3700C: c kh

nng sinh ctbolutin ccc

- 10 1200C: ctto thnhbchmli

- 4 500C: qu trnh ngng

hn

Nhit


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Company name

2.3.1. cim hnh thi

Phn b

Khpni trongt

Cc loi rau,qu, mt ong

Trong rutngi v ng

vt

Pht trinmnhtrong thcphm:hp, xc xch,c pmui,


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Company name

Cu trct bo

- Hnh que, di 2 - 10

- Di ngvi tim mao

v lng nhung

- Bo thnh budc

trung tm hoc hnhti, kch thc to hnchiu ngang catbo

2.3.2. cimcu trc


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Company name


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Company name

Vi khun gy ngcthng lC.botulinum loi A v B.Pht trinthunli t0 : 26 280C, sc

chungvi t0 cao km, bo tbnvinhit.

Pht trintt trong mi trnglng.Cch: Vi khun xm nhp vo thcn,

pht trin v sinh ct. nphithcnnhimcsbnhimct, gy ngc.


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Company name

Thi gian bnh: 6 24h.

Duhiu lm sn chyu l t litthn

kinh do tnthngthn kinh trung ngv hnh ty

Duhiu 2: c sphn ly mch v nhit

3.2. Triuchnglm sng.


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Company name

3.3. Ccloibnhngc.


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Company name

3.3.1. Ngcthcphm


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Company name

3.3.1. Ngcthcphm.


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Company name

3.3.1. Ngcthcphm.


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Company name

3.3.2. Ngctrssinh.


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Company name

3.3.2. Ngctrssinh.


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Company name

3.3.2. Ngctrssinh.


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Company name

3.3.3. Ngcdovtthng.


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Company name

3.3.3. Ngcdovtthng.


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Company name

3.3.3. Ngcdovtthng.


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Company name

4. Phnlp vnhndng.

C. botulinum thngchimmttlnh trongtngsVSV cn lm giu trc khi lm thcnghim.

Xl mu

80oC trong10p

lm giu v tochng

C.botulinum trong mtmtr nclucthtnuchn 30oC cho 7 ngy

nui cy trn mungatihoc lng

trng c b sungagar v ym kh

trong 3 ngy

kim tra csnxutctkhng

khunlcmn, 2-3mm, ngknh vimtcnhbtthngv cho thyhot phn giilypid trong lng trng trnmi trngthch (loi G ngoi

l)

Mtrncdng

Trngthi


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Company name

4. Phnlp vnhndng.

MtKthutcngin ho bngcch kthpthuc khng c vo mitrngthchsnxutct, khunlcc bao quanh bimt khu vcca cc

khng ctv lm kttavict.


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Company name

4. Phnlp vnhndng.

MU

gia nhit100oC ivi 10

pht tiu ditbtkchtc

iutrvitrypsin kch

hotbtkprotoxin

khng

ciutr

Tim vo

mngbngchut

quan st hn 4 ngy cho s pht trincatriu

chngin hnh, hithnhcxuthin


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Company name

4. Phnlp vnhndng.

Shindincactc xc nhnbng cch bovchutanngvithuckhng c v loictc thc xc

nhbng cch sdngntr khng

huyt thanh.


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Company name

5. Hiphithcphm.

1

2

3

4

Bncimnh gi snhimc Clostridium Botulinum trongthcphm l:

bnhimtu hay trong qu trnh chbin do cc bothoct bo sinh dngca Clostridium Botulinum.

b phn hy do cc vsv cnh tranh, cho nn trong iukin bnh thng nn kim sot Clostridium Botulinum.

nhit, pH, hotcanc, toiukinthunli cho s pht trinca Clostridium Botulinum

Thcphm sau khi x l nhitnh khng v

hiu ha ct.


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Company name

5. Hiphithcphm.

hp b phng 2 u np, mo m chothybnhim C.botulinum.


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Company name

5. Hiphithcphm.

Mtr axit thpca sp hp th vk rts phttrin nn kim sot nghim ngtbom anton.

hpc sx theo quy m nh trong nc thqu trnh kim tra nhm gip pht hin v ngnngammbnhchacphbinrng ri.

Tnm 1899- 1991 c 522 vngc do hp gy ra, c 432 v lin quan n rau. Anh,nhiuvngc hng lotxy ra do sdngsnphmhpca cc h gia nh.


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Company name

5. Hiphithcphm.

Ngc Chu u ( sTh/nm)1988 1989 1990 1991 1992 1993 1994 1995 1996 1997 1998a

Belgium 0 2 1 0 1 1 0 0 1 3 1Denmark 0 0 0 0 0 0 0 2 0 0 1England and Wales 0 27 0 0 0 1 1 0 0 0 2Finland 0 0 0 0 0 0 0 0 0 0 0

France 4 6 11 3 5 10 13 7 5 8 NAGermany 39 15 15 23 4 17 13 11 12 9 19Greece 0 0 0 0 0 0 0 0 0 0 0Italy 53 54 45 12 26 39 26 41 58 32 26Scotland 0 0 0 0 0 0 0 0 0 0 0Spain 8 8 10 5 12 9 7 6 7 9 11

Sweden 0 0 1 2 0 0 2 1 1 0 0The Netherlands 0 0 0 0 0 0 0 0 0 0 0

a January October.NA not available

Data for Austria, Ireland and Portugal not available

Adpated from Eurosurveillance, vol. 4, Jan 1999


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Company name

5. Hiphithcphm.

C c th nhim C. botulinum (c bit l loi E)tiu dit chng.

- Nngmuitithiu l 3% v nhit xungquanh khng di63oC trong sutqu trnh hunkhi, snphm nn cboqundi4oC.

Gianhitbngppsy

cchcamui


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Company name

5. HiPhithcphm

Nm 1960, hai dch bnh ng c loi E Bc M xy ra do sdng c hun khicng gi bng bao b ht chn khng.Nguyn nhn gy nn cc vngc l doc boqun trong chn khng, tao iukinym khthunli cho C.botulinum pht

trinmnh.


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Company name

5. Hiphithcphm.

MT S NG DNG

- Botulinum loi A iutrmtsbnhrilonvnngca chuyn khoa thn kinh, chuyn khoa

khc nhmt, tai mihng, thmm, titniu.

- Ktnm 2003, iutr cc chnglontrnglcbngctbotulin ca vo ngdngti

khoa thn kinh caBnhViniHc Y Dc TpHCM v bcupngtt nhu cuiutr

cangi dn v nhm bnh ny..


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Company name

Ktlun

Clostridium botudium l mt trong nhngnhm vi sinh vt gy bnh trong thcphm v l nguyn nhn gy ra hng lot

tvong cho con ngi.

V vycnphi tun th cch phngchngbnhcngnhvicgign v sinh

trong thcphm. Sdngthcphmngphng php v nht l thcphmnghphnchmctiangcthc

phm.


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Company name

Tmhiuvkhun Clostridium botulinum

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Clostrodium Botulinum