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Collagen Induction Therapy in Mouse( IPL vs. Micro-needling )
Hyung Sik Moon, Seong Eon Kim, Duk-Sung Ko1, Ai-Young Lee2
Department of Dermatology, Eulji University School of MedicineEulji Medical Science Laboratory1
Department of Dermatology, School of Medicine, Dongguk University2
Background
Poster 1415 months later
Poster 141
Longitudinal: 1.91mm Longitudinal: 2.44mm
5 months later
Mechanical collagen inducing • Micro-needling therapy (MTS)
Photo-rejuvenation• IPL
Object
Skin thickness (calipers)
Collagen quantitative analysis
IPL VS. micro-needling
Microscopic evaluation (MT stain)
Collagen synthesis?
Materials & Methods
1. Mouse (ICR)Total: 54 ( Control: 18 IPL: 18 MTS: 18 )
2. Chemical epilationThioglycolic acid 80% (니크린 크림TM, 일동제약, Korea)
3. IPL Intense pulsed light (Ellipse, DDD, Denmark)
4. Micro-needlingMicroneedle therapy system (DermarollerTM, Clinical resolution, USA)
5. CaliperPocket thickness gage (MituyotoTM, Mituyoto corp., Japan)
6. MT staining7. ELISA
Sircol Collagen kit (Biocolor Ltd., Belfast Northern Ireland)8. Western blot
IPL (VL-2, 10.5J, 1 pass)
0 week 2 weeks 4 weeks
Dermaroller 0.5mm (5 pass = 15 strokes)
Bx.
Skin thickness
Histology
8 weeks
ELISA
Western blot
Result
Skin thickness (um)
13812587
14613786
1538687
11012886
133132104
12810895
123110104
13210788
1179782
13311182
11311380
12810193
12711884
106100102
12710280
12386110
12912795
1139293
Micro-needlingIPLcontrol
Skin thickness
91.0±4.5 126.7±6.1
0
20
40
60
80
100
120
140
um
IPL
Control
MTS
um
140
120
100
80
60
40
20
0
110.0±7.7
p-valueControl-IPL: 0.000Control-MN: 0.000 IPL-MN: 0.001
ControlIPL
MN
Histology ( MT stain , X 100)IPL
Micro-needling
Control
Collagen quantitative analysis (ELISA)
29.942 21.253 11.791
20.448 24.741 12.180
21.909 18.642 11.790
18.916 16.298 9.757
29.178 20.425 11.032
19.683 17.554 10.253
20.225 15.165 8.636
21.080 9.756 16.111
31.921 19.176 10.796
12.921 17.606 19.957
21.886 23.094 18.218
23.768 13.702 10.749
41.100 29.545 14.101
26.460 17.425 7.470
16.108 21.776 12.275
20.877 15.069 16.027
53.300 21.957 13.683
23.600 22.298 13.873
Micro-needlingIPLcontrol
Collagen quantitative analysis(ELISA)
12.7±1.6 19.2±2.3 25.2±2.3
0.000
5.000
10.000
15.000
20.000
25.000
30.000
ug
Control
IPL
MTS
ug/m
l
30,000
25,000
20.000
15,000
10,000
5,000
0
p-valueControl-IPL: 0.000Control-MN: 0.000IPL-MN: 0.024
Control
IPLMN
Collagen quantitative analysis(Western blot)
3-C 3-I 3-M 4-C 4-I 4-M
Collagen Ⅰ
β-actin
1. Skin thickness:Control < IPL < Micro-needling
2. MT Stain (collagen fiber): Control < IPL < Micro-needling
3. Collagen quantative anaysis (ELISA,WB): Control < IPL < Micro-needling
Conclusion
Rejuvenation, Pore, Acne scar
Micro-needling
mechanical collagen inducing Tx.
Micro-needle therapy system (MTS)- Natural response to wounding the skin
Penetrate (1.5mm) Chemical cascade
Fibroblast growth factor
Transforming growth factor
Platelet derived growth factor
Collagen and elastinproduction
Collagen III (acute phase)
Collagen I (several years)
