Production of Ethanol from Potato Waste using Acremonium Cellulolyticus under Very High Gravity Conditions

Micaiah Cyril Das, Noor-ul-Ain., Sundus Khushnood, Saeed Gul

Department of Chemical Engineering, University of Engineering & Technology Peshawar, Pakistan

 micaiahcyrildas@hotmail.com , saeed.gul@uetpeshawar.edu.pk

Issues• Increase in fuel consumption. • Fossil fuel Depletion. • Economics.

SolutionRenewable Energy resourcesBio-Energy to be specific.Bio-ethanol, gas and diesel are some of the bio-fuels that can be used.

Why bio-ethanol?Worldwide production of ethanol has escalated since the last 5-10 years[1].

Project Overview

This Project will demonstrate effect of,

• Using SSF Process

• Use of A. Cellulolyticus as a single enzyme source

• Employing Very High Gravity (VHG) conditions

• Testing various nitrogen alternatives to yeast extract,on Ethanol yield

Literature

Kinetic Parameters YE PM HF FM MBM

Max. Ethanol Produced (gram per liter)

30.8 35 24.59 32.9 25.54

Max. Glucose Consumed (gram per liter)

70 73.9 53.2 68.8 53.2

Growth Rate (cfu/ml/h) 0.28 0.21 0.19 0.28 0.13Production Rate (gram per liter per hour)

3.68 3.2 1.97 3.59 2.36

Consumption Rate (gram per liter per hour)

-7.07 -5.35 -4.32 -5.06 -7.25

Yield (product/subs)*100% 43.8% 47.3% 46.2% 47.8% 48%

Table 1: Max. Ethanol Yield for different Nitrogen Sources . [3]

Figure 3: Ethanol conc. with time, during fermentation of VHG potato mash. [4]

References[1] F.O. Licht, cited in Renewable Fuels Association, Ethanol Industry Outlook 2008-

2013 reports. Available at www.ethanolrfa.org/pages/annual-industry-outlook[2] Min-Tian Gao et al., Production of ethanol from potato pulp: Investigation of the

role of the enzyme from Acremonium cellulolyticus in conversion of potato pulp into ethanol, Process Biochemistry, 2012, 47, 2110–2115.

[3] Gulten Izmirlioglu et al. , Ethanol Production from Waste Potato Mash by Using Saccharomyces Cerevisiae, Applied Sciences, 2012, 2, 738-753.

[4] Sathaporn Srichuwong et al. , Simultaneous Saccharification and fermentation (SSF) of very high gravity (VHG) potato mash for the production of ethanol, biomass and bioenergy 2009, 33, 890–898.

Visual Presentation at the COMCEDS Nationals '14 Poster Exhibition, December 17th, 2014, Lahore, Pakistan

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Comercial Trihcoderma A.Cellulolyticus0

5

10

15

20

25

30

Glu

cose

(g/

L)

Figure 2:Comparison of Glucose yield From Different Enzymes. [2]

Conclusion:It has been observed that higher rates, yields and concentration of ethanol are possible for SSF than SHF at lower enzyme loadings. Also it is possible to achieve high ethanol production using only a single microorganism and even a further yield if nitrogen source is changed. An estimate yield of 92% can be achieved.

2007 2008 2009 2010 2011 2012 20130.00

5000.00

10000.00

15000.00

20000.00

25000.00

USA Brazil EuropeChina Canada Rest of World

Bil

lion

Gal

lons

Figure 1: Global Ethanol Production by Country/Region and Year. [1]

Figure 4: Proposed Process Flow Sheet