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Confidential: For Review Only
Vegetarians have a lower risk of ischaemic heart disease
but a higher risk of stroke: results from the prospective EPIC-Oxford study
Journal: BMJ
Manuscript ID BMJ.2018.045067
Article Type: Research
BMJ Journal: BMJ
Date Submitted by the Author: 15-May-2018
Complete List of Authors: Tong, Tammy; University of Oxford, Cancer Epidemiology Unit Appleby, Paul; Oxford University, Cancer Research U.K. Epidemiology Unit, Unive Bradbury, Kathryn; University of Auckland, School of Population Health; University of Oxford, Cancer Epidemiology Unit Perez-Cornago, Aurora; Oxford University, Cancer Epidemiology Unit, Nuffield Department of Population Health Travis, Ruth; University of Oxford, Cancer Epidemiology Unit Clarke, Robert; University of Oxford Nuffield Department of Population Health Key, Timothy; University of Oxford, Cancer Epidemiology Unit
Keywords: vegetarians, cardiovascular diseases, ischaemic heart disease, stroke,
nutritional epidemiology
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Vegetarians have a lower risk of ischaemic heart disease but a higher risk 1
of stroke: results from the prospective EPIC-Oxford study 2
Tammy YN Tong, nutritional epidemiologist1, Paul N Appleby, senior statistician
1, Kathryn 3
E Bradbury, nutritional epidemiologist1, Aurora Perez-Cornago, nutritional epidemiologist
1, 4
Ruth C Travis, associate professor and senior molecular epidemiologist1, Robert Clarke, 5
professor of epidemiology and public health medicine2, Timothy J Key, professor of 6
epidemiology and deputy director1 7
1. Cancer Epidemiology Unit, Nuffield Department of Population Health, University of Oxford, Richard Doll 8
Building, Old Road Campus, Oxford , UK 9
2. Clinical Trial Service Unit and Epidemiological Studies Unit, Nuffield Department of Population Health, 10
Big Data Institute, University of Oxford, Old Road Campus, Oxford, UK 11
12
Correspondence to: Tammy YN Tong [email protected] 13
14
Word count: Abstract 241; text 3492 15
15th
May 2018 16
17
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Abstract 18
Objective: To examine the associations of vegetarianism with risks of ischaemic heart 19
disease (IHD) and stroke. 20
Design: Prospective cohort study. 21
Setting: United Kingdom cohort with a large proportion of non-meat eaters. 22
Participants: Participants were recruited in 1993-2001 and classified into four diet groups: 23
regular meat eaters (≥50 g of meat per day: n=16 332), low meat eaters (<50 g of meat per 24
day: n=8096), fish eaters (n=7506), and vegetarians including vegans (n=16 254). 25
Main outcome measures: Incident cases of IHD and stroke (including ischaemic and 26
haemorrhagic types) identified through record linkage until mid-2016. 27
Results: Over 18.1 years of follow-up, 2820 cases of IHD, 1072 cases of total stroke (519 of 28
ischaemic stroke and 300 of haemorrhagic stroke), and 3565 cases with the composite of IHD 29
or stroke were recorded. Compared with regular meat eaters, the low meat eaters, fish eaters, 30
and vegetarians had 12%, 14%, and 24% lower risks of IHD, respectively (p 31
heterogeneity<0.0001). In contrast, low meat eaters, fish eaters and vegetarians had 22%, 32
28% and 29% higher risks of stroke (p heterogeneity=0.01). Overall, compared with regular 33
meat eaters, the vegetarians had a 14% lower risk of composite IHD or stroke (hazard ratio 34
0.86, 95% CI 0.78-0.95), equivalent to 8.6 fewer cases (95% CI 4.1-12.4 fewer) per 1000 35
population over 10 years. 36
Conclusions: Low meat eaters and non-meat eaters had lower risks of IHD and higher risks 37
of stroke. Vegetarians had a lower overall risk of IHD or stroke combined. 38
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What is already known on this topic
Vegetarian and vegan diets have become increasingly popular in recent years, but the
potential benefits and hazards of these diets are not fully understood.
Vegetarians have a lower risk of ischaemic heart disease compared to non-
vegetarians.
What this study adds
This study demonstrated that low meat (<50 g of meat per day) and non-meat eaters
(fish eaters, vegetarians, vegans) had lower risks of ischaemic heart disease, but
higher risks of stroke, compared with regular meat eaters (≥50 g of meat per day).
Vegetarians had lower risks of a composite of ischaemic heart disease or stroke.
Further research is needed to confirm the findings in other populations, and to identify
which factors might mediate the observed associations.
39
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Introduction 40
Vegetarian and vegan diets have become increasingly popular in recent years partly due to 41
perceived health benefits, and concerns about the environment and animal welfare.1 In the 42
United Kingdom (UK) it has been estimated that there are close to 1.7 million vegetarians 43
and vegans.2 The European Prospective Investigation into Cancer (EPIC)-Oxford study 44
previously reported that vegetarians had a 32% lower risk of ischaemic heart disease (IHD) 45
compared to non-vegetarians.3 Some, but not all, other prospective studies reported that 46
vegetarians had lower risks of IHD mortality than non-vegetarians.4–6
For stroke, combined 47
analyses of previous studies, published 20 years ago, reported no significant differences in 48
risk of stroke deaths between vegetarians and non-vegetarians.4 49
Secular trends in cardiovascular disease outcomes, including declines in mortality from IHD 50
and stroke, in addition to differences in the proportions of stroke types,7,8
have prompted the 51
need to re-examine the associations of vegetarian diets with subtypes of cardiovascular 52
disease in a contemporary setting. The aim of the present study was to examine the 53
associations of low or non-meat diets, including vegetarian and vegan diets, with risk of IHD 54
(including acute myocardial infarction), stroke (including stroke types) and a composite of 55
either IHD or stroke events in the EPIC-Oxford study, which included a large number of non-56
meat eaters. 57
Methods 58
Study population and design 59
The EPIC-Oxford study is a prospective cohort study of approximately 65 000 men and 60
women who were recruited across the UK. Details of the recruitment process have been 61
described previously.9 Individuals were recruited from either general practice (GP) or by 62
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postal questionnaire. The GP recruitment method recruited 7421 men and women aged 35 to 63
59 years who were registered with participating GPs, all of whom completed a full 64
questionnaire on their diet, lifestyle, health characteristics and medical history. The postal 65
recruitment preferentially targeted vegetarians, vegans, and other people interested in diet and 66
health, and recruited 57 990 participants aged ≥20 years. A full questionnaire was mailed to 67
all members of the Vegetarian Society and all surviving participants of the Oxford Vegetarian 68
Study,10
and respondents were invited to provide names and addresses of relatives and friends 69
who were also interested in receiving a questionnaire. A short questionnaire was also 70
distributed to all members of the Vegan Society, enclosed in vegetarian and health food 71
magazines, and displayed in health-food shops, and a full questionnaire was subsequently 72
mailed to all those who returned the short questionnaire. 73
At recruitment, all participants were asked whether they were willing to have their blood 74
pressure measured at their general practice and to provide a blood sample. Details of the 75
procedures for blood pressure measurement and blood sample collection, which were 76
conducted in subsets of the cohort, have been previously reported.3,11,12
The study protocol 77
was approved by a Multicentre Research Ethics Committee (Scotland A Research Ethics 78
Committee) and all participants provided written informed consent. 79
Assessment of diet group 80
The full questionnaire collected responses to questions about consumption of meat, fish, dairy 81
products, and eggs, in the form of “Do you eat any meat (including bacon, ham, poultry, 82
game, meat pies, sausages)?” or similar for the other three food groups. The questionnaire 83
also included a 130-item semi-quantitative food-frequency section which asked about dietary 84
intake over the past year, including 11 questions on consumption of meat and meat products 85
which were used to estimate total meat intake. Based on responses to these questions, 86
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participants were classified into one of five diet groups: regular meat eaters (participants who 87
ate ≥50 g of total meat per day, including red meat, processed meat, and poultry); low meat 88
eaters (participants who ate <50 g of total meat per day); fish eaters (participants who did not 89
eat meat but did eat fish); vegetarians (participants who did not eat meat or fish, but did eat 90
one or both of dairy products and eggs); and vegans (participants who did not eat meat, fish, 91
dairy products, or eggs). Due to the small number of vegans, vegetarians and vegans were 92
combined as a single diet group in the main analyses, but the two groups were also examined 93
separately for each outcome in secondary analyses. 94
Outcome ascertainment 95
Participants were followed up via record linkage to National Health Service records until 31st 96
March 2016 in England, 31st May 2016 in Wales, and 31
st October 2016 in Scotland. 97
Outcomes of interest were IHD (including acute myocardial infarction, AMI), total stroke 98
(including ischaemic, haemorrhagic, or unclassified stroke), and a composite of any IHD or 99
stroke. Details of events, using the relevant ICD9 or ICD10 codes, were obtained from 100
hospital records or death certificates. 101
Exclusion criteria 102
Participants who were not resident in England, Wales, or Scotland (n=945) were excluded, as 103
were those with no Hospital Episode Statistics data or National Health Service number 104
(n=20), those who completed the short questionnaire only (n=7619), or who were younger 105
than 20 years (n=1) or older than 90 years at recruitment (n=58), had no follow-up (n=364), 106
could not be traced by the National Health Service (n=14), of unknown diet group (n=132), 107
had unreliable nutrient data (n=1219), had a self-reported history of AMI, stroke, or angina 108
(n=6837), or had a date of diagnosis which preceded or equalled the date of recruitment 109
(n=14). 110
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Statistical analyses 111
Baseline characteristics and food and nutrient intakes of the EPIC-Oxford participants were 112
summarised by diet groups. For self-reported adiposity (body mass index (BMI), waist 113
circumference), and measures of blood pressure (SBP and DBP) and blood lipids (total 114
cholesterol, HDL-C, non-HDL-C), the means and 95% confidence intervals are presented, 115
after adjustment for sex and age at entry (5 year age groups), alcohol consumption (<1g, 1-116
7g, 8-15g, 16+ g/day), and physical activity (inactive, low activity, moderately active, very 117
active, unknown)13
. 118
Cox proportional hazards regression models were used to estimate the hazard ratios (HRs) 119
and 95% confidence intervals (CIs) for the associations between diet group (regular meat 120
eaters, low meat eaters, fish eaters, vegetarians including vegans) and each outcome of 121
interest, using regular meat eaters as the reference group. The underlying time variable was 122
the age at recruitment to the age at diagnosis, death, or administrative censoring, whichever 123
occurred first. For AMI, IHD, and composite IHD or stroke, events were censored on the 124
respective outcomes of interest. For total stroke, ischaemic stroke, and haemorrhagic stroke, 125
events were censored on any stroke. All analyses were stratified by sex, method of 126
recruitment (general practice or postal), and region (7 regions across the UK), and adjusted 127
for year of recruitment (per year), education (no qualifications, basic secondary [e.g. O level], 128
higher secondary [e.g. A level], degree, unknown), Townsend deprivation index (quartiles, 129
unknown)14
, smoking (never, former, light, heavy, unknown), alcohol consumption (<1g, 1-130
7g, 8-15g, 16+ g/day), physical activity (inactive, low activity, moderately active, very active, 131
unknown), and oral contraceptive use (no, yes ever, unknown) and hormone replacement 132
therapy use (no, yes ever, unknown) in women. Likelihood ratio tests were used to test for 133
heterogeneity of risk between diet groups. Self-reported prior high blood pressure (no, yes, 134
unknown), self-reported prior high blood cholesterol (no, yes, unknown), self-reported prior 135
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diabetes (no, yes, unknown), BMI (<20, 20-22.5, 22.5-25, 25-27.5, 27.5+ kg/m2, unknown), 136
total fruit and vegetable intake (continuous), total dietary fibre (continuous), and dietary 137
supplements use (no, yes, unknown) were assessed as potential mediators by adding each 138
variable one at a time to the previous model. 139
To estimate the population impact of vegetarian diets on cardiovascular health, the absolute 140
risk difference for each outcome between low meat or meat-free diets and regular meat diets 141
was assessed. Predicted incidence and absolute risk differences were presented as per 1000 142
population over 10 years. Predicted incidence over this time period of each outcome in the 143
regular meat eaters was calculated as (1- Sr)x 1000, where Sr = (1-observed incidence in 144
regular meat eaters)10
. For all other diet groups, predicted incidence was calculated as (1-145
SrHR
)x 1000, where HR represents the hazard ratio or confidence intervals of the hazard ratio 146
for each outcome in that diet group as previously estimated, and SrHR
represents the predicted 147
10 year survival (i.e. non-incidence) rate in the diet group. Absolute risk difference was then 148
calculated as the difference between the predicted incidence per 1000 population over 10 149
years between each diet group and the regular meat eaters. 150
Additional sensitivity analyses included exclusion of events occurring during the first five 151
years of follow-up, censoring at age 70 years, setting entering time as age 70, and performing 152
multiple imputation (with 10 imputations) for missing covariates. Heterogeneity in the 153
associations between diet group and risk of IHD or stroke by sex, age at recruitment (<60 or 154
≥60 years), smoking status (never, former, or current), BMI (<25 or ≥25 kg/m2), and presence 155
of risk factors (one or more of self-reported prior high blood pressure, high blood cholesterol 156
or diabetes) was assessed by adding appropriate interaction terms to the Cox models and 157
testing for statistical significance of interaction across strata using likelihood ratio tests. All 158
analyses were performed using Stata version 14.1 or 15.1 (Stata Corp, Texas, United States) 159
and P values of <0.05 were considered significant. 160
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Results 161
Baseline characteristics 162
The baseline characteristics of the study participants for each of the four diet groups (regular 163
meat eaters, low meat eaters, fish eaters, vegetarians including vegans) are presented in Table 164
1 (and separately for vegetarians and vegans in Supplementary Table 1). Overall, compared 165
with regular meat eaters, the low or non-meat eaters were younger, had lower area-level socio 166
economic status, but were more highly educated, less likely to smoke, had lower alcohol 167
consumption, were more physically active, and more likely to report dietary supplement use. 168
They were also less likely to report prior high blood pressure, prior high blood cholesterol, 169
prior diabetes, or receive long-term treatment for illnesses. In women, low or non-meat eaters 170
were more likely to report oral contraceptive use, but less likely to report hormone 171
replacement therapy use. Compared with regular meat eaters, low or non-meat eaters reported 172
having lower body mass index and had lower measured blood pressure. For blood lipids, 173
compared with regular meat eaters, vegetarians had approximately 0.5 mmol/L lower plasma 174
concentrations of total cholesterol and non-HDL-C. Low meat eaters and fish eaters had 175
higher plasma concentrations of HDL-C compared with regular meat eaters, while 176
vegetarians had similar concentrations. 177
Food and nutrient intakes in the different diet groups are presented in Table 2 and 178
Supplementary Table 2 (separately for vegetarians and vegans). Compared with regular meat 179
eaters, vegetarians had higher intakes of fruit and vegetables, and total fibre, and lower intake 180
of saturated fat (10% vs 12% energy, respectively). 181
Association of diet groups with cardiovascular diseases 182
Over 18.1 years of follow-up in 48 188 participants, there were 2820 cases of IHD (including 183
788 cases of AMI), 1072 cases of total stroke (including 519 cases of ischaemic stroke and 184
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300 cases of haemorrhagic stroke), and 3565 cases of composite IHD or stroke events. 185
Compared with regular meat eaters, low and non-meat eaters had a lower risk of IHD (hazard 186
ratios and 95% CIs for low meat eaters: 0.88, 0.80-0.98; fish eaters: 0.86, 0.76-0.98; 187
vegetarians: 0.76, 0.68-0.85; p for heterogeneity<0.0001) (Figure 1). Conversely, vegetarians 188
had a higher risk of haemorrhagic stroke compared with regular meat eaters (1.51, 1.10-2.08), 189
and all other diet groups had a higher risk of total stroke compared with regular meat eaters 190
(low meat eaters 1.22, 1.03-1.43; fish eaters: 1.28, 1.05-1.56; vegetarians: 1.29, 1.09-1.52; p 191
for heterogeneity=0.01). There were no significant differences between diet groups for the 192
risk of AMI or ischaemic stroke. Vegetarians (0.86, 0.78-0.95), but not the other diet groups 193
(low meat eaters: 0.94, 0.86-1.03; fish eaters: 0.93, 0.83-1.04), had a significantly lower risk 194
of IHD or stroke combined compared with regular meat eaters. When vegetarians and vegans 195
were assessed separately, vegans had a lower risk of IHD (0.75, 0.58-0.97) and a borderline 196
higher risk of total stroke (1.44, 1.00-2.06) compared with regular meat eaters 197
(Supplementary Table 3). 198
When the roles of potential mediators of the associations were assessed, the associations 199
between diet group and IHD attenuated marginally when adjusted for self-reported prior high 200
blood pressure, self-reported prior high blood cholesterol, and self-reported prior diabetes, 201
and to a larger extent for BMI, but all estimates remained significant (Supplementary Table 202
4). In contrast, the association between diet group and total stroke was slightly more extreme 203
and remained significant after adjustment for self-reported prior high blood pressure, and to a 204
lesser extent when adjusted for BMI. Additional adjustment for fruit and vegetable intake, 205
total fibre, and dietary supplement use had little effect on the results. 206
Absolute risk difference for vascular disease outcomes by diet group 207
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Table 3 shows the absolute risks of IHD, total stroke, and composite of IHD and stroke 208
associated with the four diet groups. In comparing the absolute risk difference of each 209
outcome by diet groups, vegetarian diets were associated with 12.1 fewer cases (95% CI 8.4-210
15.5 fewer) of IHD per 1000 population over 10 years compared to regular meat eaters. 211
Conversely, vegetarian diets were associated with 4.3 more cases (2.0-7.0) of total stroke. 212
Overall, for total IHD or stroke, vegetarian diets were associated with 8.6 fewer cases (4.1-213
12.4 fewer) per 1000 population over 10 years when compared with regular meat eaters, 214
while the absolute risk differences between low meat eaters (-3.7; -7.5, 0.9) or fish eaters (-215
4.3; -9.7, 1.4) and regular meat eaters were not significant. 216
Sensitivity and subgroup analyses 217
In sensitivity analyses excluding events occurring during the first five years of follow-up, and 218
performing multiple imputations for missing covariates, the results were similar 219
(Supplementary table 5). When the analyses were censored at age 70 or limited to those 220
above age 70 (setting entry time as age 70) respectively, a higher risk of ischaemic stroke was 221
observed in vegetarians below the age of 70 (censored at age 70) only, while a higher risk of 222
haemorrhagic stroke was observed in low meat eaters and vegetarians above the age of 70 223
only. 224
In subgroup analyses, stronger associations were observed in men compared to women for 225
diet group and IHD (Supplementary table 6). For composite IHD or stroke, there was a 226
marginally significant heterogeneity by age at recruitment (Supplementary table 8). No other 227
significant interactions by subgroups were observed for the association between diet group 228
and IHD or stroke, either as separate outcomes, or as a composite outcome (Supplementary 229
tables 6 to 8). 230
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Discussion 231
The present study is the first to examine the association of diet groups with risk of both IHD 232
and stroke, including subtypes, in a cohort with a large proportion of non-meat eaters. Low 233
meat and meat-free diets were associated with lower risks of IHD, but higher risks of total 234
stroke. Overall, vegetarian diets were associated with lower relative and absolute risks of 235
IHD or stroke combined. The associations persisted after further adjustment for potential 236
mediators, and in most sensitivity analyses. 237
Comparison with other studies 238
In a previous report from the EPIC-Oxford study based on 11.6 years of follow-up, 239
vegetarians (including vegans) had a 32% lower risk of IHD compared with non-vegetarians 240
(meat eaters or fish eaters, HR 0.68, 0.58-0.81),3 which was consistent with the updated 241
results of the present study that included a 2-fold greater number of IHD cases and four diet 242
groups (and five in secondary analyses). In a collaborative meta-analysis of five previous 243
prospective studies (Adventist Mortality Study, Health Food Shoppers Study, Adventist 244
Health Study, Heidelberg Study, Oxford Vegetarian Study) with median recruitment from 245
between 1960 to 1980, vegetarians had a 24% lower IHD death rate ratio (0.76, 0.62-0.94) 246
compared with non-vegetarians.4 However, recent combined analyses of EPIC-Oxford and 247
Oxford Vegetarian Study data (0.99, 0.79-1.23) reported no significant difference in IHD 248
mortality,6 while analysis of 21 years of data from the German Vegetarian Study reported a 249
lower point estimate in IHD mortality in vegetarians (0.70, 0.41-1.18), though the difference 250
was not statistically significant.15
Likewise, the Adventist Health Study 2 reported that the 251
relative risks for mortality from IHD and cardiovascular disease for vegetarians versus non-252
vegetarians were 0.81 (0.64-1.02) and 0.87 (0.75-1.01), respectively,.5 253
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For stroke, previous analyses reported no significant differences in cerebrovascular disease 254
mortality by diet group.4,6
No studies were found which reported the associations of 255
vegetarian diets with the risks for incident stroke or the two subtypes of stroke. 256
Interpretation of results and implications 257
The results of the present study demonstrated that compared with regular meat eaters, all 258
other diet groups had lower risks of IHD. The reason for such differences is not certain, but it 259
may be partly attributed to lower non-HDL-C levels associated with low meat or meat-free 260
diets16–18
. Previous meta-analyses of prospective studies demonstrated that lower non-HDL-C 261
levels or low density lipoprotein cholesterol (LDL-C) levels were associated with lower 262
IHD.19,20
Furthermore, both randomised trials of statin treatments21
and Mendelian 263
randomisation studies17
have confirmed a causal association of LDL-C with IHD. Hence, it 264
is highly likely that low or non-meat eaters, who have lower non-HDL-C (Table 1) or LDL-C 265
compared to regular meat eaters11,22
, should have lower risks of IHD. 266
For stroke outcomes, combined results from two randomised statin trials reported a 21% 267
higher risk of haemorrhagic stroke per 1 mmol/L lower LDL cholesterol,16,23
which is 268
consistent with results of observational studies of cholesterol levels and haemorrhagic 269
stroke24,25
, and broadly consistent with the findings of the present study which showed that 270
vegetarians had a higher risk of haemorrhagic stroke. However, previous prospective studies 271
and randomised trials have consistently demonstrated weak positive associations of non-272
HDL-C or LDL-C levels with ischaemic stroke risk, and a non-significant or weak positive 273
association with total stroke risk.19–21,23
A recent Mendelian randomisation study also 274
reported a positive association between LDL-C and ischaemic stroke.18
This discordance 275
between previous evidence and those of the present study for LDL-C levels and ischaemic 276
stroke suggest that alternative factors probably also contribute to the observed associations. 277
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The results of several Japanese studies, showing that individuals who do not consume animal 278
products had higher incidence and mortality from haemorrhagic and total stroke, and also a 279
possibly higher risk of ischaemic stroke mortality26–29
, suggest that some factors associated 280
with animal food consumption may be protective for stroke. Vegetarians and vegans in EPIC-281
Oxford have lower circulating levels of some of several nutrients (e.g. vitamin B1230
, vitamin 282
D31
, essential amino acids32
, and long chain n-3 polyunsaturated fatty acids33
), and 283
differences in some of these nutritional factors may possibly contribute to the observed 284
associations28,34–37
. Serum concentrations of these nutritional factors and non-HDL-C have 285
only been measured in a subset of the EPIC-Oxford cohort, and therefore their role in the 286
observed associations of vegetarian diets with IHD or stroke cannot be accurately determined 287
in the current context, but should be further investigated. 288
High blood pressure is an established major risk factor for both IHD and stroke.38,39
However, 289
given that the vegetarians had lower blood pressure, and the fact that the association between 290
diet group and stroke risk became slightly stronger after adjustment for prior history of high 291
blood pressure, this is unlikely to account for the higher risks of haemorrhagic and total 292
stroke observed among vegetarians in the present study. 293
Misclassification of stroke cases, such as the misclassification of haemorrhagic stroke as 294
ischaemic stroke, cannot be excluded as a possible explanation for the discrepant results for 295
ischaemic stroke, although previous adjudication studies of stroke types confirmed the 296
reliability of hospital admission records and death certificates in the UK for diagnosis of 297
stroke types in the UK population over this calendar period.40,41
298
Strengths and limitations 299
The strengths of this study include a large sample size, a long follow-up, and outcome 300
ascertainment by linkage to medical records which minimised the loss to follow-up. The 301
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analyses included adjustment for multiple confounders, assessment of several possible 302
mediators and sensitivity analyses to confirm the robustness of the results. Among the 303
limitations of the present study, diet group was self-reported and reasons for choosing each 304
diet were not recorded. It was not possible to take into account change in diet group over the 305
years, since information required to classify all participants into regular or low meat eaters at 306
time points beyond baseline was not available. However, in a large subsample, 97% of non-307
vegetarians and 90% of vegetarians maintained their diet group at 5 year follow-up42
, 308
suggesting that long term adherence to diet group in this cohort was high. Reverse causality is 309
possible, although results were similar after excluding the first 5 years of follow-up. 310
Information on medication use (including statins) at recruitment was not available. 311
Generalisability may be limited, since the present study was based predominantly on white 312
European individuals. 313
Conclusions 314
Overall, the present study demonstrated that UK adults who consume low meat or meat-free 315
diets had lower risks of IHD, but higher risks of stroke compared with regular meat-eaters. 316
Future work should include measurements of circulating levels of cholesterol sub-fractions, 317
vitamin B12, and amino acids or fatty acids in representative samples of the different diet 318
groups to identify which factors might mediate the observed associations. Additional studies 319
in other large-scale cohorts with a high proportion of non-meat eaters are needed to confirm 320
the generalisability of these results and assess their relevance for clinical practice and public 321
health. 322
323
324
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Statements 325
Acknowledgements and funding 326
We thank all participants in the EPIC-Oxford cohort for their invaluable contribution. The 327
work is supported by the UK Medical Research Council MR/M012190/1 and Wellcome Trust 328
Our Planet Our Health (Livestock, Environment and People, LEAP 205212/Z/16/Z). KEB is 329
supported by the Girdlers’ New Zealand Health Research Council Fellowship. 330
Conflicts of interest 331
TJK is a member of the Vegan Society; the other authors had no conflicts of interest. The 332
funders had no role in the design of the study; in the collection, analyses, or interpretation of 333
data; in the writing of the manuscript; or in the decision to publish the results. 334
Availability of data and materials 335
The data access policy for EPIC-Oxford is available via the study website (http://www.epic-336
oxford.org/data-access-sharing-and-collaboration/). 337
Author’s contributions 338
TYNT and TJK conceived and designed the research question. TYNT analysed the data and 339
wrote the first draft of the manuscript, and PNA, KEB, APC, RCT, RC and TJK provided 340
input on data analysis and interpretation of results. All authors revised the manuscript 341
critically for important intellectual content, and read and approved the final manuscript. 342
Transparency declaration 343
The lead author affirms that this manuscript is an honest, accurate, and transparent account of 344
the study being reported; that no important aspects of the study have been omitted; and that 345
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any discrepancies from the study as planned (and, if relevant, registered) have been 346
explained.347
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Table 1: Baseline characteristics of participants in different diet groups in EPIC-Oxford (n=48 188).
Diet groups
Characteristics
Regular meat
eaters1
Low meat eaters1 Fish eaters Vegetarians and
vegans
Mean (SD) or n (%) (n=16 332) (n=8096) (n=7506) (n=16 254)
Socio-demographic
Age, years 49.7 (12.8) 47.7 (13.5) 42.1 (12.8) 39.4 (13.1)
Sex, women (%) 12 048 (73.8) 6433 (79.5) 6186 (82.4) 12 232 (75.3)
Top socio-economic quartile
(%)2 4239 (29.5) 1720 (24.8) 1431 (21.9) 3018 (21.2)
Higher education (%) 4216 (25.8) 3158 (39.0) 3308 (44.1) 6698 (41.2)
Lifestyle
Current smokers, (%) 2074 (12.7) 881 (10.9) 764 (10.2) 1685 (10.4)
Alcohol consumption, g/day 10.5 (13.4) 9.2 (11.6) 10.0 (12.3) 9.3 (12.8)
Moderate/ high physical
Activity, (%) 4250 (26.0) 2502 (30.9) 2684 (35.8) 5849 (36.0)
Dietary supplement use, (%)3 8396 (52.4) 4899 (62.0) 4702 (64.1) 8961 (56.1)
Medical history, n (%)
Prior high blood pressure 2111 (13.0) 827 (10.3) 549 (7.3) 935 (5.8)
Prior high blood cholesterol 1174 (7.2) 442 (5.5) 255 (3.4) 345 (2.1)
Prior diabetes, 271 (1.7) 82 (1.0) 61 (0.8) 93 (0.6)
Receiving long term
treatment for any illness 4962 (30.8) 2060 (25.8) 1622 (21.9) 3077 (19.1)
Oral contraceptive use4 8635 (72.1) 4628 (72.4) 4959 (80.5) 9620 (79.0)
Hormone replacement
therapy use4 3199 (26.9) 1285 (20.3) 728 (11.9) 954 (7.9)
Physical measurements, mean
(95% CI)5
Body mass index (kg/m2) 24.4 (24.4, 24.5) 23.4 (23.3, 23.5) 23.1 (23.0, 23.2) 23.0 (23.0, 23.1)
Systolic blood pressure
(mmHg) 126.3 (125.8, 126.7) 124.4 (123.7, 125.0) 123.4 (122.6, 124.2) 123.7 (123.1, 124.2)
Diastolic blood pressure
(mmHg) 77.3 (77.1, 77.6) 76.5 (76.1, 76.9) 75.5 (75.0, 76.0) 75.9 (75.6, 76.2)
Total cholesterol (mmol/L) 5.52 (5.47, 5.57) 5.44 (5.35, 5.52) 5.31 (5.23, 5.39) 4.97 (4.92, 5.03)
HDL cholesterol (mmol/L) 1.31 (1.29, 1.33) 1.35 (1.32, 1.38) 1.35 (1.32, 1.38) 1.29 (1.28, 1.31)
Non-HDL cholesterol
(mmol/L) 4.22 (4.17, 4.27) 4.08 (4.00, 4.17) 3.96 (3.88, 4.04) 3.68 (3.62, 3.74)
Estimates shown are mean (SD), n (%), or adjusted means (95% confidence interval), as stated in left column. For all
variables, p-heterogeneity between diet groups was<0.001.
1 Regular meat eaters were defined as participants with total meat consumption ≥50 g per day, and low meat eaters were
defined as participants with total meat consumption <50 g per day. 2 Based on Townsend index. 3 Defined as regularly taking any vitamins, minerals, fish oils, fibre or other food supplements during the last 12 months. 4 In women only. 5 Body mass index and waist circumference were based on self-reported measures in the whole cohort. Blood pressure was
measured in 6519 regular meat eaters, 2343 low meat eaters, 1742 fish eaters, and 4364 vegetarians and vegans. Blood lipids
were measured in 1487 regular meat eaters, 498 low meat eaters, 566 fish eaters, and 1109 vegetarians and vegans. Estimates
were adjusted for the cross-stratification of gender and age at entry (5 year age groups), alcohol consumption (<1g, 1-7g, 8-
15g, 16+ g/day), and physical activity (inactive, low activity, moderately active, very active, unknown).
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Table 2: Food and nutrient intakes of participants in different diet groups in EPIC-Oxford (n=48 188).
Diet groups
Foods or nutrients Regular meat
eaters1
Low meat eaters1 Fish eaters Vegetarians and
vegans (n=16 332) (n=8096) (n=7506) (n=16 254)
Foods
Total meat and meat
products (g/day) 100.1 (41.5) 28.2 (12.9) --- ---
Red and processed meat (g/day) 65.9 (38.4) 18.8 (10.3) --- ---
Poultry (g/day) 34.2 (22.1) 9.4 (5.6) --- ---
Total fish (g/day) 43.6 (28.8) 38.4 (29.3) 38.5 (33.3) ---
Dairy milk (ml/day) 336.5 (183.3) 299.2 (184.9) 274.0 (189.8) 232.3 (207.3)
Soya milk (ml/day) 3.7 (34.4) 10.4 (56.7) 21.1 (79.4) 54.5 (127.9)
Dairy cheese (g/day) 19.7 (17.5) 22.8 (20.4) 27.5 (24.2) 26.8 (25.4)
Total fresh fruit (g/day) 249.7 (187.9) 295.2 (239.3) 291.1 (228.0) 283.8 (239.2)
Total vegetables (g/day) 248.7 (123.1) 259.3 (147.6) 287.3 (148.7) 294.4 (163.2)
Nutrients
Carbohydrates (% energy) 46.8 (5.9) 50.4 (6.3) 51.0 (6.5) 52.8 (6.8)
Protein (% energy) 17.6 (2.9) 15.6 (2.5) 14.6 (2.3) 13.6 (2.1)
Total fat (% energy) 32.1 (5.6) 30.5 (6.2) 30.8 (6.3) 30.2 (6.6)
Saturated fat (% energy) 11.8 (3.2) 10.9 (3.4) 10.6 (3.3) 10.2 (3.5)
Monounsaturated fat (% energy) 11.0 (2.2) 10.0 (2.4) 9.9 (2.4) 9.7 (2.6)
Polyunsaturated fat (% energy) 6.3 (1.8) 6.4 (2.2) 7.0 (2.2) 7.1 (2.5)
Total fibre (g/day) 18.4 (6.4) 19.5 (7.4) 21.2 (7.4) 22.1 (8.0)
Total energy (kJ/day) 8,633 (2,227) 7,624 (2,141) 7,939 (2,199) 7,813 (2,234)
Estimates shown are mean (SD). For all variables, p-heterogeneity between diet groups was<0.001.
1 Regular meat eaters were defined as participants with total meat consumption ≥50 g per day, and low meat eaters were
defined as participants with total meat consumption <50 g per day.
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Figure 1: Prospective associations between diet groups (regular and low meat eaters, fish eaters, vegetarians and vegans)
and risk of ischaemic heart disease and stroke in EPIC-Oxford (n=48 188).
Regular meat eaters were defined as participants with total meat consumption ≥50 g per day, and low meat eaters were defined as
participants with total meat consumption <50 g per day. Total analyses times were 848 969 person-years for acute myocardial
infarction, 836 980 person-years for ischaemic heart disease, 848 840 person-years for ischaemic stroke, haemorrhagic stroke, or
total stroke, and 833 604 person-years for ischaemic heart disease or stroke. All analyses were stratified by sex, method of
recruitment (general practice or postal), and region (7 categories), and adjusted for year of recruitment (per year), education (no
qualifications, age 16, age 18, degree, unknown), Townsend deprivation index (quartiles, unknown), smoking (never, former,
light, heavy, unknown), alcohol consumption(<1g, 1-7g, 8-15g, 16+ g/day), physical activity (inactive, low activity, moderately
active, very active, unknown), and oral contraceptive and hormone replacement therapy use in women. P-heterogeneity represents
significance of heterogeneity in risk between diet groups based on likelihood ratio tests. Box sizes are proportional to the number
of cases in each group.
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Table 3: Absolute risk difference (per 1000 population over 10 years) of ischaemic heart disease
and stroke in different diet groups in EPIC-Oxford.
Outcome and diet groups Predicted incidence per
1000 population over 10
years1
Absolute risk difference
per 1000 population over
10 years2
Ischaemic heart disease
Regular meat eaters3 51.3 (48.4, 54.4) Reference
Low meat eaters3 45.3 (41.8, 49.2) -6.0 (-9.4, -2.0)
Fish eaters 44.3 (39.7, 49.5) -7.0 (-11.6, -1.7)
Vegetarians and vegans 39.2 (35.8, 42.8) -12.1 (-15.5, -8.4)
Total stroke
Regular meat eaters3 15.1 (13.6, 16.8) Reference
Low meat eaters3 18.4 (16.2, 20.8) 3.3 (1.1, 5.7)
Fish eaters 19.3 (16.3, 22.7) 4.2 (1.2, 7.6)
Vegetarians and vegans 19.5 (17.1, 22.1) 4.3 (2.0, 7.0)
Ischaemic heart disease or stroke
Regular meat eaters3 63.0 (59.8, 66.3) Reference
Low meat eaters3 59.3 (55.5, 63.8) -3.7 (-7.5, 0.9)
Fish eaters 58.7 (53.3, 64.4) -4.3 (-9.7, 1.4)
Vegetarians and vegans 54.4 (50.6, 58.8) -8.6 (-12.4, -4.1)
1 For regular meat eaters, this was calculated as (1- Sr)x 1000, where Sr = (1-observed incidence in
regular meat eaters)10
, and represents the predicted 10 year survival rate in the regular meat eaters.
For all other diet groups, this was calculated as (1- SrHR
)x 1000, where HR represents the hazard ratio
or confidence intervals of the hazard ratio for each outcome in that diet group, and SrHR
represents the
predicted 10 year survival (i.e. non-incidence) rate in the diet group. Confidence intervals in all diet
groups were estimated based on floating absolute risk. 2 Calculated as the difference between the predicted incidence per 1000 population over 10 years
between each diet group and the regular meat eaters. 3 Regular meat eaters were defined as participants with total meat consumption ≥50 g per day, and low
meat eaters were defined as participants with total meat consumption <50 g per day.
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References
1. Leahy E, Lyons S, Tol RSJ. An estimate of the number of vegetarians in the world
[Internet]. ESRI working paper 340. 2010 [cited 2018 May 14]. Available from:
https://www.esri.ie/publications/an-estimate-of-the-number-of-vegetarians-in-the-
world/
2. The Vegan Society. Find out how many vegans are in Great Britain [Internet]. 2016
[cited 2018 Jan 29]. Available from: https://www.vegansociety.com/whats-
new/news/find-out-how-many-vegans-are-great-britain
3. Crowe FL, Appleby PN, Travis RC, Key TJ. Risk of hospitalization or death from
ischemic heart disease among British vegetarians and nonvegetarians: results from the
EPIC-Oxford cohort study. Am J Clin Nutr. 2013;97:597–603.
4. Key TJ, Fraser GE, Thorogood M, et al. Mortality in vegetarians and nonvegetarians:
detailed findings from a collaborative analysis of 5 prospective studies. Am J Clin
Nutr. 1999;70:516S–524S.
5. Orlich MJ, Singh PN, Sabaté J, et al. Vegetarian dietary patterns and mortality in
Adventist Health Study 2. JAMA Intern Med. 2013;173:1230–8.
6. Appleby PN, Crowe FL, Bradbury KE, Travis RC, Key TJ. Mortality in vegetarians
and comparable nonvegetarians in the United Kingdom. Am J Clin Nutr.
2016;103:218–30.
7. Lawlor DA, Smith GD, Leon DA, Sterne JAC, Ebrahim S. Secular trends in mortality
by stroke subtype in the 20th century: a retrospective analysis. Lancet.
2002;360:1818–23.
Page 22 of 35
https://mc.manuscriptcentral.com/bmj
BMJ
123456789101112131415161718192021222324252627282930313233343536373839404142434445464748495051525354555657585960
Confidential: For Review Only
23
8. British Heart Foundation. Cardiovascular disease statistics 2017 [Internet]. 2017 [cited
2018 Feb 14]. Available from: https://www.bhf.org.uk/research/heart-statistics/heart-
statistics-publications/cardiovascular-disease-statistics-2017
9. Davey GK, Spencer EA, Appleby PN, et al. EPIC-Oxford: lifestyle characteristics and
nutrient intakes in a cohort of 33 883 meat-eaters and 31 546 non meat-eaters in the
UK. Public Health Nutr. 2003;6:259–68.
10. Appleby PN, Thorogood M, Mann JI, Key TJ. The Oxford Vegetarian Study: an
overview. Am J Clin Nutr. 1999;70:525S–531S.
11. Bradbury KE, Crowe FL, Appleby PN, et al. Serum concentrations of cholesterol,
apolipoprotein A-I and apolipoprotein B in a total of 1694 meat-eaters, fish-eaters,
vegetarians and vegans. Eur J Clin Nutr. 2014;68:178–83.
12. Appleby PN, Davey GK, Key TJ. Hypertension and blood pressure among meat eaters,
fish eaters, vegetarians and vegans in EPIC-Oxford. Public Health Nutr. 2002;5:645–
54.
13. Wareham NJ, Jakes RW, Rennie KL, et al. Validity and repeatability of a simple index
derived from the short physical activity questionnaire used in the European
Prospective Investigation into Cancer and Nutrition (EPIC) study. Public Health Nutr.
2003;6:407–13.
14. Townsend P. Poverty in the United Kingdom: A Survey of Household Resources and
Standards of Living. Allen Lane and Penguin Books. London, UK; 1979.
15. Chang-Claude J, Hermann S, Eilber U, Steindorf K. Lifestyle determinants and
mortality in German vegetarians and health-conscious persons: results of a 21-year
follow-up. Cancer Epidemiol Biomarkers Prev. 2005;14:963–8.
Page 23 of 35
https://mc.manuscriptcentral.com/bmj
BMJ
123456789101112131415161718192021222324252627282930313233343536373839404142434445464748495051525354555657585960
Confidential: For Review Only
24
16. Collins R, Reith C, Emberson J, et al. Interpretation of the evidence for the efficacy
and safety of statin therapy. Lancet. 2016;388:2532–61.
17. Ference BA, Yoo W, Alesh I, et al. Effect of long-term exposure to lower low-density
lipoprotein cholesterol beginning early in life on the risk of coronary heart disease: a
mendelian randomization analysis. J Am Coll Cardiol. 2012;60:2631–9.
18. Hindy G, Engström G, Larsson SC, et al. Role of blood lipids in the development of
ischemic stroke and its subtypes: a mendelian randomization study. Stroke.
2018;49:820–7.
19. MacMahon S, Duffy S, Rodgers A, et al. Blood cholesterol and vascular mortality by
age, sex, and blood pressure: a meta-analysis of individual data from 61 prospective
studies with 55 000 vascular deaths. Lancet. 2007;370:1829–39.
20. Emerging Risk Factors Collaboration, Di Angelantonio E, Sarwar N, et al. Major
lipids, apolipoproteins, and risk of vascular disease. JAMA. 2009;302:1993–2000.
21. Cholesterol Treatment Trialists’ (CTT) Collaboration, Baigent C, Blackwell L, et al.
Efficacy and safety of more intensive lowering of LDL cholesterol: a meta-analysis of
data from 170,000 participants in 26 randomised trials. Lancet. 2010;376:1670–81.
22. Thorogood M, Carter R, Benfield L, McPherson K, Mann JI. Plasma lipids and
lipoprotein cholesterol concentrations in people with different diets in Britain. Br Med
J. 1987;295:351–3.
23. Amarenco P, Bogousslavsky J, Callahan A, et al. High-dose atorvastatin after stroke or
transient ischemic attack. N Engl J Med. 2006;355:549–59.
24. Iso H, Jacobs DR, Wentworth D, Neaton JD, Cohen JD. Serum cholesterol levels and
Page 24 of 35
https://mc.manuscriptcentral.com/bmj
BMJ
123456789101112131415161718192021222324252627282930313233343536373839404142434445464748495051525354555657585960
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25
six-year mortality from stroke in 350,977 men screened for the multiple risk factor
intervention trial. N Engl J Med. 1989;320:904–10.
25. Yano K, Reed DM, MacLean CJ. Serum cholesterol and hemorrhagic stroke in the
Honolulu Heart Program. Stroke. 1989;20:1460–5.
26. Kinjo Y, Beral V, Akiba S, et al. Possible protective effect of milk, meat and fish for
cerebrovascular disease mortality in Japan. J Epidemiol. 1999;9:268–74.
27. Sauvaget C, Nagano J, Allen N, Grant EJ, Beral V. Intake of animal products and
stroke mortality in the Hiroshima/Nagasaki Life Span Study. Int J Epidemiol.
2003;32:536–43.
28. Takeya Y, Popper JS, Shimizu Y, et al. Epidemiologic studies of coronary heart
disease and stroke in Japanese men living in Japan, Hawaii and California: incidence
of stroke in Japan and Hawaii. Stroke. 1977;15:15–23.
29. Sauvaget C, Nagano J, Hayashi M, Yamada M. Animal protein, animal fat, and
cholesterol intakes and risk of cerebral infarction mortality in the adult health study.
Stroke. 2004;35:1531–7.
30. Gilsing AMJ, Crowe FL, Lloyd-Wright Z, et al. Serum concentrations of vitamin B12
and folate in British male omnivores, vegetarians and vegans: results from a cross-
sectional analysis of the EPIC-Oxford cohort study. Eur J Clin Nutr. 2010;64:933–9.
31. Crowe FL, Steur M, Allen NE, et al. Plasma concentrations of 25-hydroxyvitamin D in
meat eaters, fish eaters, vegetarians and vegans: results from the EPIC-Oxford study.
Public Health Nutr. 2011;14:340–6.
32. Schmidt JA, Rinaldi S, Scalbert A, et al. Plasma concentrations and intakes of amino
Page 25 of 35
https://mc.manuscriptcentral.com/bmj
BMJ
123456789101112131415161718192021222324252627282930313233343536373839404142434445464748495051525354555657585960
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26
acids in male meat-eaters, fish-eaters, vegetarians and vegans: a cross-sectional
analysis in the EPIC-Oxford cohort. Eur J Clin Nutr. 2016;70:306–12.
33. Rosell MS, Lloyd-Wright Z, Appleby PN, et al. Long-chain n-3 polyunsaturated fatty
acids in plasma in British meat-eating, vegetarian, and vegan men. Am J Clin Nutr.
2005;82:327–34.
34. Dong H, Pi F, Ding Z, et al. Efficacy of supplementation with B vitamins for stroke
prevention: a network meta-analysis of randomized controlled trials. PLoS One.
2015;10:1–12.
35. Iso H, Stampfer MJ, Manson JE, et al. Prospective study of fat and protein intake and
risk of intraparenchymal hemorrhage in women. Circulation. 2001;103:856–63.
36. Sacks FM, Lichtenstein AH, Wu JHY, et al. Dietary fats and cardiovascular disease: a
presidential advisory from the American Heart Association. Circulation. 2017;136:e1–
23.
37. McGreevy C, Williams D. New insights about vitamin D and cardiovascular disease: a
narrative review. Ann Intern Med. 2011;155:820–6.
38. Lim SS, Vos T, Flaxman AD, et al. Global, regional, and national comparative risk
assessment of 79 behavioural, environmental and occupational, and metabolic risks or
clusters of risks in 188 countries, 1990-2013: a systematic analysis for the Global
Burden of Disease Study 2013. Lancet. 2016;380:2224–60.
39. Rosendorff C, Lackland DT, Allison M, et al. Treatment of hypertension in patients
with coronary artery disease: a scientific statement from the American Heart
Association, American College of Cardiology, and American Society of Hypertension.
Circulation. 2015;131:e435–70.
Page 26 of 35
https://mc.manuscriptcentral.com/bmj
BMJ
123456789101112131415161718192021222324252627282930313233343536373839404142434445464748495051525354555657585960
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27
40. Sinha S, Myint PK, Luben RN, Khaw K-T. Accuracy of death certification and
hospital record linkage for identification of incident stroke. BMC Med Res Methodol.
2008;8:74.
41. Wright FL, Green J, Canoy D, et al. Vascular disease in women: comparison of
diagnoses in hospital episode statistics and general practice records in England. BMC
Med Res Methodol. 2012;12:161.
42. Key TJ, Appleby PN, Spencer EA, et al. Mortality in British vegetarians: results from
the European Prospective Investigation into Cancer and Nutrition. Am J Clin Nutr.
2009;1960:1–4.
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Supplementary table 1: Baseline characteristics of vegetarians and vegans in EPIC-Oxford.
Diet groups
Characteristics Vegetarians Vegans
Max n=14 422 Max n=1832
Socio-demographic
Age, years (SD) 39.5 (13.1) 38.6 (13.2)
Sex, women (%) 11 063 (76.7) 1169 (63.8)
Top socio-economic quartile (%)1 2728 (21.6) 290 (18.0)
Degree education (%) 5949 (41.2) 749 (40.9)
Lifestyle
Current smokers (%) 1484 (10.3) 201 (11.0)
Alcohol consumption, g/day (SD) 9.4 (12.7) 8.5 (13.7)
Moderate/ high physical activity (%) 5088 (35.3) 761 (41.5)
Dietary supplement use, (%)2 8026 (56.6) 935 (52.1)
Medical history, n (%)
Prior high blood pressure (% yes) 850 (5.9) 85 (4.6)
Prior high blood cholesterol (% yes) 317 (2.2) 28 (1.6)
Prior diabetes (% yes) 86 (0.6) 7 (0.4)
Receiving long term treatment for any
illness (% yes) 2778 (19.4) 299 (16.4)
Oral contraceptive use (% yes)3 8791 (79.8) 829 (71.2)
Hormone replacement therapy use
(% yes)3 887 (8.1) 67 (5.9)
Physical measurements, mean (95% CI)4
Body mass index (kg/m2) 23.1 (23.1, 23.2) 22.3 (22.1, 22.4)
Systolic blood pressure (mmHg) 124.0 (123.4, 124.5) 121.7 (120.5, 123.0)
Diastolic blood pressure (mmHg) 76.1 (75.8, 76.5) 74.4 (73.6, 75.2)
Total cholesterol (mmol/L) 5.10 (5.03, 5.17) 4.76 (4.67, 4.85)
HDL cholesterol (mmol/L) 1.30 (1.27, 1.32) 1.29 (1.26, 1.32)
Non-HDL cholesterol (mmol/L) 3.80 (3.73, 3.87) 3.47 (3.38, 3.56)
Estimates shown are mean (SD), n (%), or adjusted means (95% confidence interval), as stated in left column.
1 Based on Townsend index.
2 Defined as regularly taking any vitamins, minerals, fish oils, fibre or other food supplements during the last 12 months.
3 In women only.
4 Body mass index and waist circumference were based on self-reported measures in the whole cohort. Blood lipids were
measured in 674 vegetarians and 435 vegans, and blood pressure was measured in 3688 vegetarians and 676 vegans.
Estimates were adjusted for the cross-stratification of gender and age at entry (5 year age groups), alcohol consumption (<1g,
1-7g, 8-15g, 16+ g/day), and physical activity (inactive, low activity, moderately active, very active, unknown).
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Supplementary table 2: Food and nutrient intakes of vegetarians and vegans in EPIC-Oxford.
Diet groups
Foods or nutrients Vegetarians Vegans
Max n=14 422 Max n=1832
Foods
Dairy milk (ml/day) 260.6 (202.3) ---
Soya milk (ml/day) 32.7 (98.1) 228.4 (189.7)
Dairy cheese (g/day) 30.0 (25.1) ---
Total fresh fruit (g/day) 275.8 (221.0) 346.2 (345.1)
Total vegetables (g/day) 288.0 (157.8) 345.1 (193.4)
Nutrients
Carbohydrates (% energy) 52.5 (6.6) 55.4 (7.8)
Protein (% energy) 13.6 (2.1) 13.3 (2.3)
Total fat (% energy) 30.5 (6.5) 28.1 (7.3)
Saturated fat (% energy) 10.7 (3.4) 6.5 (2.1)
Monounsaturated fat (% energy) 9.7 (2.5) 9.4 (3.4)
Polyunsaturated fat (% energy) 6.8 (2.3) 9.1 (2.9)
Total fibre (g/day) 21.6 (7.7) 25.9 (9.3)
Total energy (kJ/day) 7,871 (2,213) 7,359 (2,346)
Estimates shown are mean (SD).
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Supplementary table 3. Prospective associations between 5 diet groups and risk of cardiovascular diseases in EPIC-Oxford
(n=48,188).
Outcome and diet groups1 N Cases Hazard ratios (95%
confidence intervals)2
P-heterogeneity2
Acute myocardial infarction
Regular meat eaters 16 332 399 Reference
Low meat eaters 8096 152 0.99 (0.82,1.20)
Fish eaters 7506 85 1.00 (0.78,1.28)
Vegetarians 14 422 137 0.93 (0.75,1.15)
Vegans 1832 15 0.72 (0.42,1.21) 0.76
Ischaemic heart disease
Regular meat eaters 16 332 1462 Reference
Low meat eaters 8096 535 0.88 (0.80,0.98)
Fish eaters 7506 307 0.86 (0.76,0.98)
Vegetarians 14 422 453 0.76 (0.68,0.85)
Vegans 1832 63 0.75 (0.58,0.97) <0.0001
Ischaemic stroke
Regular meat eaters 16 332 218 Reference
Low meat eaters 8096 115 1.17 (0.93,1.48)
Fish eaters 7506 62 1.11 (0.82,1.48)
Vegetarians 14 422 106 1.19 (0.93,1.54)
Vegans 1832 18 1.58 (0.96,2.59) 0.31
Haemorrhagic stroke
Regular meat eaters 16 332 108 Reference
Low meat eaters 8096 64 1.37 (1.00,1.89)
Fish eaters 7506 41 1.33 (0.91,1.94)
Vegetarians 14 422 79 1.55 (1.12,2.13)
Vegans 1832 8 1.24 (0.59,2.59) 0.09
Total stroke
Regular meat eaters 16 332 434 Reference
Low meat eaters 8096 232 1.22 (1.03,1.43)
Fish eaters 7506 142 1.28 (1.05,1.56)
Vegetarians 14 422 230 1.27 (1.07,1.51)
Vegans 1832 34 1.44 (1.00,2.06) 0.02
Ischaemic heart disease or stroke
Regular meat eaters 16 332 1799 Reference
Low meat eaters 8096 714 0.94 (0.86,1.03)
Fish eaters 7506 414 0.93 (0.83,1.04)
Vegetarians 14 422 637 0.86 (0.78,0.95)
Vegans 1832 92 0.90 (0.72,1.11) 0.05 1 For diet group, regular meat eaters were defined as participants with total meat consumption ≥50 g per day, and low meat
eaters were defined as participants with total meat consumption <50 g per day. 2 Total analyses time was 848 969 person-years for acute myocardial infarction, 836 980 person-years for ischaemic heart
disease, 848 840 person-years for ischaemic stroke, haemorrhagic stroke, or total stroke, and 833 604 person-years for
ischaemic heart disease or stroke. All analyses were stratified by sex, method of recruitment (general practice or postal), and
region (7 categories), and adjusted for year of recruitment (per year), education (no qualifications, age 16, age 18, degree,
unknown), Townsend deprivation index (quartiles, unknown), smoking (never, former, light, heavy, unknown), alcohol
consumption(<1g, 1-7g, 8-15g, 16+ g/day), physical activity (inactive, low activity, moderately active, very active,
unknown), and oral contraceptive and hormone replacement therapy use in women. P-heterogeneity represents significance of
heterogeneity in risk between diet groups based on likelihood ratio tests.
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Supplementary table 4. Prospective associations between diet groups and risk of cardiovascular diseases in EPIC-Oxford, further adjusted for potential mediators of the
associations (n =48,188).
Hazard ratios (95% confidence intervals) with additional adjustment for2
Outcome and diet
groups1
Self-reported
prior high blood
pressure
Self-reported
prior high blood
cholesterol
Self-reported
prior diabetes
BMI3 Fruit and
vegetable intake4
Total fibre4 Supplement use
5
Ischaemic heart disease
Regular meat eaters Reference Reference Reference Reference Reference Reference Reference
Low meat eaters 0.90 (0.81,1.00) 0.89 (0.80,0.98) 0.90 (0.81,0.99) 0.92 (0.84,1.02) 0.88 (0.80,0.98) 0.88 (0.80,0.98) 0.88 (0.80,0.97)
Fish eaters 0.89 (0.78,1.01) 0.87 (0.77,0.99) 0.87 (0.77,0.99) 0.93 (0.82,1.06) 0.86 (0.76,0.98) 0.87 (0.77,0.99) 0.86 (0.75,0.98)
Vegetarians and vegans 0.80 (0.71,0.89) 0.78 (0.70,0.87) 0.78 (0.70,0.87) 0.82 (0.74,0.92) 0.76 (0.68,0.85) 0.77 (0.69,0.86) 0.76 (0.68,0.85)
P-heterogeneity2 0.0006 0.0001 0.0001 0.007 <0.0001 0.0001 <0.0001
Total stroke
Regular meat eaters Reference Reference Reference Reference Reference Reference Reference
Low meat eaters 1.23 (1.04,1.45) 1.22 (1.03,1.43) 1.22 (1.04,1.44) 1.23 (1.04,1.45) 1.21 (1.03,1.43) 1.22 (1.03,1.43) 1.22 (1.04,1.44)
Fish eaters 1.31 (1.07,1.59) 1.27 (1.04,1.55) 1.29 (1.06,1.57) 1.30 (1.06,1.59) 1.27 (1.04,1.54) 1.28 (1.04,1.56) 1.29 (1.06,1.57)
Vegetarians and vegans 1.34 (1.13,1.59) 1.28 (1.08,1.52) 1.31 (1.10,1.55) 1.31 (1.11,1.56) 1.27 (1.07,1.51) 1.29 (1.08,1.53) 1.29 (1.09,1.53)
P-heterogeneity2 0.002 0.01 0.005 0.005 0.01 0.01 0.007
Ischaemic heart disease
or stroke
Regular meat eaters Reference Reference Reference Reference Reference Reference Reference
Low meat eaters 0.96 (0.88,1.05) 0.95 (0.87,1.04) 0.95 (0.87,1.04) 0.98 (0.90,1.07) 0.94 (0.86,1.03) 0.94 (0.86,1.03) 0.94 (0.86,1.03)
Fish eaters 0.96 (0.86,1.07) 0.94 (0.84,1.05) 0.94 (0.84,1.05) 0.99 (0.88,1.10) 0.93 (0.83,1.04) 0.94 (0.84,1.05) 0.93 (0.83,1.04)
Vegetarians and vegans 0.90 (0.82,0.99) 0.88 (0.80,0.97) 0.88 (0.80,0.97) 0.92 (0.84,1.01) 0.86 (0.78,0.95) 0.87 (0.79,0.96) 0.86 (0.79,0.95)
P-heterogeneity2 0.22 0.07 0.08 0.39 0.02 0.05 0.02
1 For diet group, regular meat eaters were defined as participants with total meat consumption ≥50 g per day, and low meat eaters were defined as participants with total meat consumption
<50 g per day. 2 Total analyses time was 836 980 person-years for IHD, 848 932 person-years for total stroke, and 833 604 person-years for IHD or stroke. All analyses were stratified by sex, method of
recruitment (general practice or postal), and region (7 categories), and adjusted for year of recruitment (per year), education (no qualifications, age 16, age 18, degree, unknown),
Townsend deprivation index (quartiles, unknown), smoking (never, former, light, heavy, unknown), alcohol consumption(<1g, 1-7g, 8-15g, 16+ g/day), physical activity (inactive, low
activity, moderately active, very active, unknown), and oral contraceptive and hormone replacement therapy use in women. P-heterogeneity represents significance of heterogeneity in risk
between diet groups based on likelihood ratio tests. 3 BMI was adjusted for categorically (<20, 20-22.5, 22.5-25, 25-27.5, 27.5+ kg/m
2, unknown).
4 Fruit and vegetable intake and total fibre were adjusted for continuously.
5 Supplement use was adjusted for categorically (no, yes, unknown).
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Supplementary table 5: Sensitivity analyses on prospective association between diet group and risk of ischaemic heart diseases and stroke.
Hazard ratios (95% confidence intervals)1
Outcome and diet groups Excluding first 5 years of
follow-up
N=46 757
Censoring at age 70
N=45 894
Setting entry time at age 70
N=13 875
Multiple imputation for
covariates (10 datasets)
N=48 188
Acute myocardial infarction
Regular meat eaters2 Reference Reference Reference Reference
Low meat eaters2 1.08 (0.88,1.33) 0.93 (0.68,1.27) 1.02 (0.80,1.31) 0.99 (0.81,1.20)
Fish eaters 1.01 (0.77,1.32) 1.12 (0.79,1.59) 0.89 (0.62,1.26) 0.99 (0.77,1.26)
Vegetarians and vegans 0.93 (0.74,1.17) 0.77 (0.56,1.05) 0.98 (0.74,1.30) 0.90 (0.73,1.11)
P-heterogeneity1 0.71 0.21 0.90 0.81
Ischaemic heart disease
Regular meat eaters2 Reference Reference Reference Reference
Low meat eaters2 0.90 (0.81,1.01) 0.83 (0.70,0.97) 0.92 (0.81,1.05) 0.88 (0.80,0.98)
Fish eaters 0.84 (0.73,0.97) 0.81 (0.67,0.98) 0.91 (0.76,1.08) 0.86 (0.76,0.98)
Vegetarians and vegans 0.77 (0.68,0.86) 0.71 (0.61,0.84) 0.75 (0.65,0.88) 0.76 (0.68,0.85)
P-heterogeneity1 0.0001 0.0002 0.005 <0.0001
Ischaemic stroke
Regular meat eaters2 Reference Reference Reference Reference
Low meat eaters2 1.23 (0.97,1.56) 1.12 (0.69,1.81) 1.20 (0.92,1.56) 1.17 (0.93,1.48)
Fish eaters 1.12 (0.82,1.51) 1.39 (0.85,2.29) 0.99 (0.68,1.43) 1.12 (0.83,1.50)
Vegetarians and vegans 1.20 (0.93,1.55) 1.53 (1.01,2.33) 1.06 (0.78,1.44) 1.24 (0.97,1.58)
P-heterogeneity1 0.31 0.21 0.56 0.31
Haemorrhagic stroke
Regular meat eaters2 Reference Reference Reference Reference
Low meat eaters2 1.38 (0.97,1.95) 1.04 (0.65,1.66) 1.76 (1.14,2.72) 1.38 (1.00,1.90)
Fish eaters 1.21 (0.79,1.85) 1.19 (0.73,1.93) 1.36 (0.73,2.53) 1.32 (0.91,1.94)
Vegetarians and vegans 1.53 (1.08,2.17) 1.07 (0.70,1.62) 2.16 (1.35,3.46) 1.51 (1.10,2.07)
P-heterogeneity1 0.08 0.92 0.007 0.06
Total stroke
Regular meat eaters2 Reference Reference Reference Reference
Low meat eaters2 1.26 (1.06,1.50) 1.19 (0.88,1.61) 1.23 (1.02,1.50) 1.22 (1.03,1.43)
Fish eaters 1.20 (0.96,1.49) 1.46 (1.06,2.00) 1.16 (0.90,1.51) 1.28 (1.05,1.56)
Vegetarians and vegans 1.28 (1.06,1.53) 1.31 (0.99,1.73) 1.21 (0.97,1.49) 1.29 (1.09,1.53)
P-heterogeneity1 0.02 0.10 0.13 0.009
Ischaemic heart disease or stroke
Regular meat eaters2 Reference Reference Reference Reference
Low meat eaters2 0.97 (0.88,1.06) 0.88 (0.77,1.02) 0.98 (0.88,1.10) 0.94 (0.86,1.03)
Fish eaters 0.89 (0.79,1.01) 0.92 (0.79,1.09) 0.93 (0.80,1.09) 0.93 (0.83,1.04)
Vegetarians and vegans 0.86 (0.78,0.96) 0.82 (0.71,0.94) 0.85 (0.75,0.97) 0.86 (0.79,0.95)
P-heterogeneity1 0.03 0.03 0.10 0.03
1 Total N for each sensitivity analysis was based on N in the analyses for ischaemic heart disease or stroke if numbers varied. The total analyses time for the composite outcome of
ischaemic heart disease and stroke in each sensitivity analyses is as follows: excluding first 5 years of follow-up=595 809 person-years; censoring at age 70=727 797 person-years;
limiting to participants above age 70=118 690 person-years; multiple imputation=833 604 person-years. P-heterogeneity represents significance of heterogeneity in risk between diet
groups based on likelihood ratio tests. 2 Regular meat eaters were defined as participants with total meat consumption ≥50 g per day, and low meat eaters were defined as participants with total meat consumption <50 g per day.
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Supplementary table 6: Risk of ischaemic heart disease by subgroups in different diet groups in EPIC-Oxford.
Hazard ratios (95% confidence intervals)1 Test of difference
between subgroups4 Subgroups of variable
Sex Men Women
N cases 1176 1644
Regular meat eaters2 Reference Reference
Low meat eaters2 0.73 (0.62,0.87) 0.98 (0.87,1.11)
Fish eaters 0.75 (0.60,0.93) 0.94 (0.80,1.10) χ2=9.57
Vegetarians and vegans 0.69 (0.59,0.81) 0.81 (0.70,0.93) p=0.023
P-heterogeneity1 p<0.0001 p=0.026
Age at recruitment <60 years ≥60 years
N cases 1367 1453
Regular meat eaters2 Reference Reference
Low meat eaters2 0.83 (0.71,0.97) 0.93 (0.81,1.06)
Fish eaters 0.77 (0.64,0.92) 0.96 (0.80,1.15) χ2=5.09
Vegetarians and vegans 0.69 (0.59,0.80) 0.84 (0.72,0.98) p=0.17
P-heterogeneity1 p<0.0001 p=0.15
Body mass index <25 kg/m2 ≥25 kg/m
2
N cases 1454 1366
Regular meat eaters2 Reference Reference
Low meat eaters2 0.96 (0.83,1.10) 0.91 (0.78,1.05)
Fish eaters 0.96 (0.81,1.13) 0.91 (0.74,1.12) χ2=0.75
Vegetarians and vegans 0.85 (0.74,0.98) 0.78 (0.65,0.93) p=0.86
P-heterogeneity1 p=0.15 p=0.044
Smoking status Never Former Current
N cases 1419 1020 366
Regular meat eaters2 Reference Reference Reference
Low meat eaters2 0.90 (0.78,1.03) 0.93 (0.79,1.10) 0.69 (0.51,0.94)
Fish eaters 0.82 (0.68,0.98) 0.95 (0.78,1.16) 0.80 (0.56,1.14) χ2=4.59
Vegetarians and vegans 0.76 (0.65,0.87) 0.80 (0.67,0.95) 0.66 (0.48,0.92) p=0.60
P-heterogeneity1 p=0.001 p=0.078 p=0.016
Presence of risk factors3 No Yes
N cases 1833 965
Regular meat eaters2 Reference Reference
Low meat eaters2 0.88 (0.77,1.00) 0.95 (0.81,1.13)
Fish eaters 0.85 (0.73,1.00) 1.04 (0.83,1.29) χ2=4.12
Vegetarians and vegans 0.77 (0.67,0.87) 0.93 (0.77,1.13) P=0.25
P-heterogeneity1 p=0.0006 p=0.82
1 All analyses were stratified by sex, method of recruitment (general practice or postal), and region (7 categories), and adjusted
for year of recruitment (per year), education (no qualifications, age 16, age 18, degree, unknown), Townsend deprivation
index (quartiles, unknown), smoking (never, former, light, heavy, unknown), alcohol consumption(<1g, 1-7g, 8-15g, 16+
g/day), physical activity (inactive, low activity, moderately active, very active, unknown), and oral contraceptive and
hormone replacement therapy use in women. P-heterogeneity represents significance of heterogeneity in risk between diet
groups based on likelihood ratio tests. 2 Regular meat eaters were defined as participants with total meat consumption ≥50g per day, and low meat
eaters were defined as participants with total meat consumption <50g per day. 3 Presence of one or more of self-reported prior high blood pressure, high blood cholesterol, or diabetes.
4 Test of interaction by sex, age at recruitment, body mass index, smoking, and presence of risk factors were performed by
adding appropriate interaction terms to the Cox models, and testing for statistical significance of interaction across strata
using likelihood ratio tests.
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Supplementary table 7: Risk of stroke by subgroups in different diet groups in EPIC-Oxford.
Hazard ratios (95% confidence intervals)1 Test of difference
between subgroups4 Subgroups of variable
Sex Men Women
N cases 304 768
Regular meat eaters2 Reference Reference
Low meat eaters2 1.30 (0.96,1.76) 1.18 (0.98,1.43)
Fish eaters 1.31 (0.88,1.94) 1.26 (1.01,1.58) χ2=2.84
Vegetarians and vegans 1.09 (0.81,1.47) 1.36 (1.13,1.66) p=0.42
P-heterogeneity1 p=0.28 p=0.012
Age at recruitment <60 years ≥60 years
N cases 405 667
Regular meat eaters2 Reference Reference
Low meat eaters2 1.02 (0.76,1.36) 1.32 (1.08,1.60)
Fish eaters 1.26 (0.94,1.70) 1.26 (0.97,1.64) χ2=3.00
Vegetarians and vegans 1.15 (0.89,1.49) 1.39 (1.12,1.73) p=0.39
P-heterogeneity1 p=0.42 p=0.006
Body mass index <25 kg/m2 ≥25 kg/m
2
N cases 659 413
Regular meat eaters2 Reference Reference
Low meat eaters2 1.13 (0.92,1.40) 1.42 (1.09,1.84)
Fish eaters 1.31 (1.03,1.66) 1.29 (0.89,1.87) χ2=3.31
Vegetarians and vegans 1.18 (0.96,1.46) 1.53 (1.15,2.04) P=0.35
P-heterogeneity1 p=0.14 p=0.008
Smoking status Never Former Current
N cases 605 364 97
Regular meat eaters2 Reference Reference Reference
Low meat eaters2 1.41 (1.15,1.74) 0.95 (0.71,1.27) 0.93 (0.52,1.67)
Fish eaters 1.39 (1.07,1.80) 1.06 (0.76,1.48) 1.35 (0.73,2.51) χ2=8.12
Vegetarians and vegans 1.26 (1.01,1.57) 1.29 (0.99,1.68) 1.40 (0.83,2.37) P=0.23
P-heterogeneity1 p=0.005 p=0.20 p=0.46
Presence of risk factors3 No Yes
N cases 740 322
Regular meat eaters2 Reference Reference
Low meat eaters2 1.12 (0.91,1.37) 1.45 (1.10,1.91)
Fish eaters 1.32 (1.05,1.66) 1.24 (0.84,1.83) χ2=5.20
Vegetarians and vegans 1.24 (1.02,1.50) 1.73 (1.27,2.34) P=0.16
P-heterogeneity1 p=0.06 p=0.002
1 All analyses were stratified by sex, method of recruitment (general practice or postal), and region (7 categories), and adjusted
for year of recruitment (per year), education (no qualifications, age 16, age 18, degree, unknown), Townsend deprivation
index (quartiles, unknown), smoking (never, former, light, heavy, unknown), alcohol consumption(<1g, 1-7g, 8-15g, 16+
g/day), physical activity (inactive, low activity, moderately active, very active, unknown), and oral contraceptive and
hormone replacement therapy use in women. P-heterogeneity represents significance of heterogeneity in risk between diet
groups based on likelihood ratio tests. 2 Regular meat eaters were defined as participants with total meat consumption ≥50 g per day, and low
meat eaters were defined as participants with total meat consumption <50 g per day. 3 Presence of one or more of self-reported prior high blood pressure, high blood cholesterol, or diabetes.
4 Test of interaction by sex, age at recruitment, body mass index, smoking, and presence of risk factors were performed by
adding appropriate interaction terms to the Cox models, and testing for statistical significance of interaction across strata
using likelihood ratio tests.
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Supplementary table 8: Risk of IHD or stroke by subgroups in different diet groups in EPIC-Oxford.
Hazard ratios (95% confidence intervals)1 Test of difference
between subgroups4 Subgroups of variable
Sex Men Women
N cases 1401 2255
Regular meat eaters2 Reference Reference
Low meat eaters2 0.83 (0.71,0.96) 1.01 (0.91,1.13)
Fish eaters 0.84 (0.69,1.02) 0.98 (0.86,1.12) χ2=7.74
Vegetarians and vegans 0.76 (0.66,0.88) 0.93 (0.83,1.05) p=0.052
P-heterogeneity1 p=0.0009 p=0.61
Age at recruitment <60 years ≥60 years
N cases 1719 1937
Regular meat eaters2 Reference Reference
Low meat eaters2 0.86 (0.75,0.98) 1.01 (0.90,1.14)
Fish eaters 0.85 (0.73,1.00) 1.00 (0.85,1.16) χ2=8.05
Vegetarians and vegans 0.77 (0.67,0.87) 0.97 (0.85,1.11) p=0.045
P-heterogeneity1 p=0.0006 p=0.95
Body mass index <25 kg/m2 ≥25 kg/m
2
N cases 1986 1670
Regular meat eaters2 Reference Reference
Low meat eaters2 0.98 (0.87,1.11) 1.00 (0.87,1.14)
Fish eaters 1.01 (0.88,1.16) 0.96 (0.80,1.17) χ2=0.25
Vegetarians and vegans 0.92 (0.82,1.04) 0.91 (0.78,1.07) p=0.97
P-heterogeneity1 p=0.53 p=0.69
Smoking status Never Former Current
N cases 1897 1294 445
Regular meat eaters2 Reference Reference Reference
Low meat eaters2 1.01 (0.90,1.14) 0.93 (0.80,1.08) 0.69 (0.52,0.91)
Fish eaters 0.93 (0.80,1.09) 0.92 (0.77,1.10) 0.92 (0.68,1.26) χ2=7.20
Vegetarians and vegans 0.86 (0.76,0.98) 0.89 (0.77,1.04) 0.77 (0.58,1.02) P=0.30
P-heterogeneity1 p=0.09 p=0.43 p=0.04
Presence of risk factors3 No Yes
N cases 2434 1192
Regular meat eaters2 Reference Reference
Low meat eaters2 0.92 (0.82,1.03) 1.04 (0.89,1.20)
Fish eaters 0.93 (0.82,1.07) 1.05 (0.86,1.28) χ2=5.16
Vegetarians and vegans 0.87 (0.78,0.97) 1.07 (0.90,1.27) P=0.16
P-heterogeneity1 p=0.07 p=0.86
1 All analyses were stratified by sex, method of recruitment (general practice or postal), and region (7 categories), and adjusted
for year of recruitment (per year), education (no qualifications, age 16, age 18, degree, unknown), Townsend deprivation
index (quartiles, unknown), smoking (never, former, light, heavy, unknown), alcohol consumption(<1g, 1-7g, 8-15g, 16+
g/day), physical activity (inactive, low activity, moderately active, very active, unknown), and oral contraceptive and
hormone replacement therapy use in women. P-heterogeneity represents significance of heterogeneity in risk between diet
groups based on likelihood ratio tests. 2 Regular meat eaters were defined as participants with total meat consumption ≥50 g per day, and low
meat eaters were defined as participants with total meat consumption <50 g per day. 3 Presence of one or more of self-reported prior high blood pressure, high blood cholesterol, or diabetes.
4 Test of interaction by sex, age at recruitment, body mass index, smoking, and presence of risk factors were performed by
adding appropriate interaction terms to the Cox models, and testing for statistical significance of interaction across strata
using likelihood ratio tests.
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