4.0 6.0

ABA1/2/3L/4

8.0 10.0 12.0 14.0 16.0

●

●●

●

●

●

●

ERG12 ERG10 Hygro ERG13tHMG1

ABA3L ABA1 KanMX ABA4ABA2

Empty vectors

ABA3L

Time (min)200 250 300 350 nm

0

1000

2000

3000

4000

232

278

Build FPP upregulating

construct

Reconstitute Botrytis cinerea

ABA pathway

Integrate constructs into

sYL12

Confirm ABA biosynthesis

(LC-MS)

Activate ABA sensor with

endogenous ABA

Deactivate ABA sensor with CYP707A1

L 1 2 3 4 5 6 7

2 3 4 5 6

L 2-3

3-4

4-5

5-6

L 3 4 L 5 6

L2+

33+

44+

55+

6