COST850 29 Salzau2 Martinez de Altube

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    Maria del Mar Martinez de AltubeMaria del Mar Martinez de Altube

    Al n r M r in z P& Ale andro Martinez Pea

    Control of the flatheaded rootborerControl of the flatheaded rootborer((Capnodis tenebrionisCapnodis tenebrionis) with the) with the

    entomopathogenic nematodeentomopathogenic nematodeSteinernema carpocapsaeSteinernema carpocapsae

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    MEDITERRANEAN FLATHEADED ROOTBORER

    Capnodis tenebrionis(Coleoptera: Buprestidae)

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    CHERRYAPRICOTNECTARINEPEACH

    ALMONDPLUM

    APPLEPEAR

    IN OFF

    FLATHEADED ROOTBORER PESTFLATHEADED ROOTBORER PEST

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    24.000 ha

    21.000 ha

    33.000 ha

    70.000 ha

    APRICOT CHERRY

    PEACH + NECTARINE

    CURRENT SITUATION SPAIN

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    CapnodisCapnodis tenebrionistenebrionis

    Larvae

    Egg

    Neonate larvae

    Adult

    AAAAAAAAPPPPLLLLLLLLLLLL2 YEAR2 YEAR

    LLLLLLLLLLLLEEAAAAAAAAAA1 YEAR1 YEARDDNNOOSSAAJJJJMMAAMMFFJJ

    upae

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    PEST SYMPTOMSPEST SYMPTOMS

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    PEST SYMPTOMSPEST SYMPTOMSRESIN ON THE TRUNK

    LACK OF NEW SHOOTS

    GALLERIES AND HOLES IN THETRUNK

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    CHITOSAN + EPN

    N-acetyl-glucosamine

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    +

    PEST CONTROL PLANT RECOVERY

    EPN

    Activates plant defencePromotes root development

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    ObjectiveObjective fieldfield teststests

    DetermineDetermine effecteffect ofofBiorendBiorend RR againstagainst

    CapnodisCapnodistenebrionistenebrionis inin apricotapricot ((PrunusPrunusarmeniacaarmeniaca))

    WeWe testedtested thethe effecteffect ofof

    1.1. DoseDose

    2.2.ApplicationApplication techniquetechnique

    3. Time3. Time forfor applicationapplication

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    1. DOSES TESTED1. DOSES TESTED

    1=1= 11 MillionMillion EPN + 0,6 gEPN + 0,6 g chitosanchitosan// treetree..

    1,5=1,5= 1.51.5

    MillionMillion

    EPN + 0,9 gEPN + 0,9 g

    chitosanchitosan

    //

    treetree

    .

    .

    DOSESDOSES

    NematodesNematodes

    --chitosanchitosan

    //

    treetree

    (S.carpocapsae, SSA3)

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    2. APPLICATIONS TESTED2. APPLICATIONS TESTED

    1.1. DrenchDrench. (. (WaterWater aroundaround thethe treetree).).

    2.2. InjectionInjection aroundaround thethe treetree

    3.3.DripDrip irrigationirrigation

    CDRENCH

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    DRENCHDRENCH

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    INJECTION APPLICATIONINJECTION APPLICATION

    InjectorInjector

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    DRIP APPLICATIONDRIP APPLICATION11 -- 44 dripdrip holesholes aroundaround thethe treetree..

    18l18l perper treetree// hourhour

    inin summersummer 2h/2h/dayday

    36l/36l/treetree ++ SolutionSolution ((nematodes+chitosannematodes+chitosan))

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    0

    5

    10

    15

    20

    25

    30

    CONTROL D INJ 1 INJ 2

    0

    10

    20

    30

    40

    50

    60

    70

    80

    90

    100

    alive nematode infected efficacyF = 11.65, df= 3, 8, P= 0.0027

    25.6

    25.6

    8.3

    3.3

    5

    80%

    9.2

    5.6

    3.6

    78%

    3.3

    4

    84%

    7.3

    ControlControl ofofC.tenebrionisC.tenebrioniswithwith

    BiorendBiorend RR

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    EFFECTS

    After 1 year

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    EFFECTS

    After 1 year

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    ConclusionConclusion

    BiorendBiorend

    RR

    givegive

    excellentexcellent

    controlcontrol

    RentabilityRentability ofof orchardsorchards maintainedmaintained