CREST Program at CUWCREST Students1.2011 Group modeled Herpes viral peptide complexed to HLA-A2

Demonstrating Class I MHC presentationLearned viral evasion of class I MHC presentation and how it is investigatedPaid summer stipend for student to gain research experience in Dr. Hudson’s lab. Student is now working in a research lab and is applying to graduate school

2.2012 Group modeled HCMV US2 complexed to HLA-A2 presenting viral peptide.Demonstrating protein interactions between HLA-A2 and US2, a viral protein thatsubverts class I MHC presentationLearned viral evasion of class I MHC presentation and how it is investigatedStudents developing poster for presentation at ASMCUE in JuneTwo of the students plan on working to develop story for cellular landscape of

class I MHC presentation and herpes viral evasion

Researcher: Dr. Amy HudsonEducator: Dr. Ann McDonald

CREST Educator: Ann H. McDonaldCREST students take 1 semester ‘Project Modeling’ courseBiology Research Scholars Program through ASM (July, 2011 - June, 2012) Focus on developing cellular landscape Summer, 2012Fall, 2012 CREST students will model TCR for peptide on HLA-A2

Future Modeling/Teaching ProjectsModeling Immunological Synapse on T cells – TCR, CD4/CD8, CD3, , CD28, CD2, LFA-1Modeling Immunological Synapse on DC – peptide-HLA-A2, B7, LFA-3, ICAM-1Find projects for lower level classes in Microbiology

Classroom Use of ModelsUsing JMOL modeling to teach protein structure in ImmunologyDesigned group project using 3D models of Abs, Ig-foldHLA-A2 model used to help understand class I MHC presentation Group project on modeling proteins in immunological synapse

Class I MHC presentation of Viral Proteins to CD8+ CTL