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Cry3A transgenic, bark beetle resistant spruce. NAZV project QH71290 2007-2011. Spruce bark beetle (Ips typographus). Bark beetle galleries. Bacillus thuringiensis crystal protein (Cry, δ -endoTOXin). Agrobacterium interaction with the plant cell. Major problems:. - PowerPoint PPT Presentation
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Cry3A transgenic, bark Cry3A transgenic, bark beetle resistant sprucebeetle resistant spruce
NAZV project NAZV project QH71290QH712902007-20112007-2011
Spruce bark beetle (Ips typographus)
Bark beetle galleries
Bacillus thuringiensiscrystal protein (Cry, δ-endoTOXin)
Agrobacterium interaction with the plant cell
• Spruce is difficult to transformSpruce is difficult to transform
• No known Bt toxin is toxic enough to bark No known Bt toxin is toxic enough to bark beetlebeetle
Major problems:Major problems:
Spruce callus cultures
Somatic embryogenesis
induction conversion cultivation
Modified Cry3A genes
Cry3A orig
Synt TOX-1 (602 aa)-codons Picea optimized
Ndel/sTOX-1
Cdel/sTOX-1 (602 aa)
Cdel/Ndel/sTOX-1 GUS
Refined modification of domain II
Expression vector
PAGE analysis of modified Cry3A expression
WESTERN detection of modified Cry3A proteins
Toxicity tests on live beetles
K o n e c p r e s e n t a c e