culture - bioMérieux España...chromID CPS® agar, an isolation medium for urine specimens, is fully compatible with 23 identiﬁcation reagents and with 10 AST reagents including

GET A culture AHEAD

PrODuct INSIGHT

LIST OF MIcrO-OrGANISMS

LIST OF culture MeDIA AND ADDITIONAL PRODUCTS

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GET A CULTURE AHEADAt bioMérieux, our commitment to bringing you innovative culture

media solutions is based on a family history that goes back over a

century. For generations, the Mérieux family has remained dedicated

to improving public health by advancing the fi eld of Microbiology

through a winning combination of science and industry.

In the early 1970’s, Alain Mérieux’ vision was to create the world’s fi rst automated culture media pro-duction facility, in Craponne, France. The building of this facility resulted in a wave of innovation in industrial lab automation and new technologies. The possibility of having standardized, cost-effective pre-plated media started a revolution in laboratory workfl ow and quality. This bioMérieux production site is now the largest culture media facility in Europe.

Conscious of the power of automation, bioMérieux has continued to push back the limits of laboratory effi ciency with FMLA*, bringing innovations such as PREVI™ Isola into your diagnostic and industrial labs for faster, more reliable test results and better patient management.

Committed to helping you meet your daily challenges, we’ve made some unique advances in microbio-logy. That’s why this is the only catalogue where you’ll fi nd a range of innovative chromogenic media: chromID™. And in addition to the required controls, ALL our media are backed by our unique Compatibility and Thermal Shock state-ment, because our idea of service is to make your life easier by ensuring the quality of our products.

We wish you happy reading as you discover our Culture of commitment in the pages that follow.

PIONEERS IN MICROBIOLOGY AND LABORATORY AUTOMATION

* Full Microbiology Laboratory Automation™

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culture OF PATIENT CARE

culture OF SERVICE

culture OF QUALITY

culture OF INNOVATION

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INFECTIOUS DISEASES, A WORLDWIDE CONCERN

In today’s global environment, the emergence and rapid transmission of infectious diseases has become a major public health issue. Recognized as vital for the prevention of infectious diseases, in vitro diagnostic tools for patient management and surveillance have become an essential component of healthcare systems worldwide.

With over 100 years experience in bacteriology, the history of bioMérieux is directly linked to the fight against infectious diseases; today, this continues to be the Company’s strategic focus. At bioMérieux,

the patient is at the core of our strategic vision: we are committed to helping you deliver the right information to the right people more rapidly for better clinical outcomes.

Backed by our longstanding tradition and know-how as a world leader in this field, we are continually pushing back the frontiers of disease detection, providing you with complete diagnostic solutions to fight antibiotic resistance, healthcare-associated infections, respiratory infections and septicaemia.

Time-to-diagnosis and accuracy of treatment are paramount to clinical decision making and ultimately to patient health outcomes. As a microbiology laboratory, your primary objective is to deliver the most pertinent information to doctors as quickly as possible, so patients can receive the most appropriate treatments. Rapid, accurate in vitro diagnostic tests help you achieve this. They play a crucial role in patient management, as well as reducing healthcare costs and contributing to the fight against the development of multidrug resistant bacteria.

THE LABORATORY: A PIVOTAL ROLE IN HEALTHCARE

CULTURE of PATIENT CARE

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WE ARE COMMITTED TO PROVIDING COMPLETE DIAGNOSTIC SOLUTIONS, FOR EXAMPLE:For the prevention of group B streptococcalinfections in pregnant women and newborns v with a complete culture media range from specimens to susceptibility testing - in alignment with CDC* Guidelines - that can be combined to provide solutions to fit different laboratory needs.

For the prevention of nosocomial infections, such as C. difficile v with a rapid, cost-effective solution designed to help confirm infection, identify the origin, track the emergence of antibiotic resistance and support effective epidemiological monitoring.

THE LABORATORY: A PIVOTAL ROLE IN HEALTHCARE

DIAGNOSTIC SOLUTIONS FOR LIFE-LONG HEALTHCARE

bioMérieux, A WORLD LEADER IN IN VITRO DIAGNOSTICS

With over 45 years experience at the forefront of in vitro diagnostics,

our unique know-how and expertise make bioMérieux a reference

in clinical microbiology.

Our innovative solutions for diagnosing infectious diseases provide

high medical value information – helping to optimize therapeutic

decision-making for better patient care.

IN THE WORLD OF MICROBIOLOGY, bioMérieux REPRESENTS

• The worldwide reference in in vitro diagnostics

• The most extensive line of chromogenic culture media available

• Diagnostic solutions recognized by the international scientific community

* Centers for Disease Control and Prevention

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A GLOBAL VISION WITH LOCAL SOLUTIONS

Thanks to our international presence, we offer innovative

products that have been tested and approved worldwide,

while our local networks meet your most specific needs.

This dual ‘local/global’ aspect guarantees that you will always

have the right culture media, when and where you need it.

CULTURE of SERVICE

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For bioMérieux, being a leader means a commitment to providing you with only the very best products and services. To ensure you receive first class assistance, we have set-up a reactive local Customer Service network of specialists who understand your needs. At bioMérieux, there is always a micro- biologist ready to answer your questions!

WE ALSO OFFER:n a complete on-line Technical Library where you can download all Package Inserts, Quality Control Certificates, Material Safety Data Sheets (MSDS), Non-hazardous Product Statements, Thermal Shock Statements and Compatibility Certificates

n product training, led by our in-house trainers with specific sessions on ‘Quality’ and ‘Accreditation’

n bioMérieux “Education days”, on current themes, led by recognized experts in the field

n local microbiology training in your language: France, Germany, Greece, Poland, Spain, Portugal, …

n workflow audits, carried out by our experts to streamline your organization and enhance your productivity

n E-learning courses on our products

THE CERTIFICATE OF COMPATIBILITY – MAKING YOUR DAILY LIFE EASIERSetting-up new tests and acquiring accreditation can be costly and time-consuming. To help lighten your workload, bioMérieux verifies the compatibility of its product ranges and provides with each medium – free of charge – a Certificate of Compatibility: a validated document showing its compatibility with complementary bioMérieux tests.

As a result, we can certify that, for example, chromID™ CPS® agar, an isolation medium for urine specimens, is fully compatible with 23 identification reagents and with 10 AST reagents including VITEK®2 cards, API®, ATB™ strips and rapid tests.

The Certificate of Compatibility is a ready-to-use document that can be easily downloaded from the bioMérieux website Technical Library to your lab accreditation file.

Visit our website www.biomerieux.com/techlib

BEHIND EACH MEDIA, A FULL RANGE OF SERVICES

AT bioMérieux, SERVICE IS

• Being there to answer your questions and provide solutions

• A dedicated team of microbiologists

• Technicians and microbiologists trained in our centre

• A commitment to customer satisfaction

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As microbiologists, we understand the increasingly demanding regulatory context laboratories face today and take an active approach to helping you meet your quality requirements. It is our policy to make sure you have the highest, reproducible quality products and services. We employ a rigorous Quality Assurance System that complies with the strictest standards, giving you the transparency and information you need.

Our Quality System is certified ISO 9001(2000) for all its activities and NF EN ISO 13485 (2004) for clinical activities, including culture media. Our products comply with Directive 98/79/CE (1998/10/27) relative to medical devices for in vitro diagnostic use and must meet strict security and safety requirements.

We also comply with several related norms to ensure consistent product quality, user and patient safety, and a limited environmental impact.

Each product is accompanied by a bioMérieux Quality Control certificate, providing you with complete traceability throughout the production process. This puts all the controls performed – from raw materials up to the finished product – in your hands and ensures inter-lot reproducibility.

Going a step further, we recognise that maximum flexibility in the daily work routine of laboratory technicians can translate into being a culture ahead – while a lack of flexibility can leave you a culture behind. We are therefore the only company to provide a “Thermal Shock Statement” guaranteeing the shelf-life of your culture media for 1 to 4 weeks at 15-25 °C (see table 1).

QUALITY BY bioMérieux, A TOTAL COMMITMENT

CULTURE of QUALITY

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SHELF-LIFE AND STORAGE TEMPERATURE Beyond testing our products for conformity with the regulation on “Stability testing of in vitro diagnostic reagents” we also validate their stability in a real-life situation in the lab: 4-week storage at 15-25°C.

This is to ensure we offer maximum flexibility in the daily work routine of laboratory technicians.

STORAGE TEMPERATURE VALIDATIONThermal shocks are variations in temperature that products may be subjected to during production or transportation. The shelf-life of our culture media is determined by real-time stability studies in which the media undergoes simulations of a break in the cold chain, including storage at 15-25°C.

The stability study consists of checking the conformity of appearance, pH, and bacterio- logical activity.

THE bioMérieux“TOTAL QUALITY CHAIN”

EXAMPLE OF A THERMAL SHOCK PROTOCOL FOR PREPLATED MEDIA:

Storage: •2-8°C •10-15°Cfor4weeks •18-25°Cfor6days,including35-39°Cfor8hours •15-25°Cfor4weeks •2-8°C

AT bioMérieux, QUALITY IS

• Dedicated quality control experts across our 8 production sites

• ISO 9001 (2000) certification for all our activities

• ISO 13485 (2004) certification for all clinical activities

• 110 000 technical documents available at www.biomerieux.com/techlib: quality control and comptability certificates…

nNFEN13612«Performanceevaluationofinvitrodiagnosticmedicaldevices»(2002),onthemanagement,assessmentanddocumentationofmanu-facturerperformanceevaluations.nNFEN12322«Culturemediaformicrobiology/Performancecriteriaforculturemedia»(1999),ontheperformanceofculturemediaused inmicrobiology labs(traceability,compatibility, reproducibilityofproductperformance throughout theproduct’s lifecycleandsuitability). nISO23640(2012)Evaluationofstabilityofin vitrodiagnosticreagents.nNFEN375«Informationprovidedbythemanufacturerwithin vitrodiagnosticreagentsforprofessionaluse»(2001),concerninginformationprovidedbythemanufacturerofin vitrodiagnosticreagents.nNFEN1041 «Informationsuppliedby themanufacturerwithmedicaldevices»(2008),concerning informationprovidedby themanufacturerofmedicaldevicescovered bytheEUDirectives.nNFEN980«Graphicalsymbolsforuseinthelabellingofmedicaldevices»(2008),ongraphicalsymbolsusedoninformationprovidedbythemanufacturerofmedicaldevices.nISO15223«Symbolstobeusedwithmedicaldevicelabels,labeling,andinformationtobesupplied+ amendments» (2010), concerning symbols conventionally used to convey information essential for the safe and effective useofmedical devices. nDirective94/62/CE«Packagingandpackagingwaste»(1994),ontheharmonizationofthevariousnationalmeasuresrelativetopackagingandpackagingwaste,tooffermaximalenvironmentalprotectionandensurethefreecirculationofgoodsbetweenmembercountries.

PACKAGE INSERTS: providecleardetailsontheperformanceofeachculturemedium.

QUALITY CONTROL CERTIFICATES:forcompletetraceabilityoftheproductionprocess,indicatingperformancecriteriaandcollectionstrainsusedfortheQualityControlofeachlotofculturemedia.

KIT LABELLING: informationonshelf-life, storagetemperatureandguaranteeofcompliancewithECRegulations,usingclearandeasysymbolsforallusers.

PACKAGING: specificallydesignedtolimitenvironmentalimpactandhelpyoureduceyourwastevolumeandweight.

Time

Temperature

Storage temperature

Thermal shocks including 4 weeks storage at 15-25°CTABLE 1

Control conform Control not conform Shelf-life

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PARTNERSHIPS FOR PROGRESS: COLLABORATION AND NETWORKINGAs a leader on the cutting edge, we work closely with innovators in the public and private sectors: research laboratories, community hospitals, biotech-nology companies, the pharmaceutical industry, and the information technology and imaging industries. Through these stimulating collaborations, we actively engage in knowledge and experience sharing across several areas. The result is the application of novel approaches and technologies, bringing improved diagnostics to your laboratory.

Just one example is a long-term strategic partnership with the French Atomic Energy Commission (CEA), launched in 2010. Together with the CEA, we are developing new image processing and spectroscopy technologies to improve infectious disease diagnosis and management. Multidisciplinary programs are underway, with a focus on rapid bacterial detection and identification methods. Significant steps have already been made in the field of mass spectroscopy, paving the way for future diagnostic advances.

Culture time is currently the longest step in the diagnostic process. Imagine if you could cut back on culture time by reading microcolonies, which are undetectable to the human eye. With new image processing technologies integrated in an innovative module, we are pushing back the limits of automated Petri dish reading to bring the Petri dish into the digital age, while conserving the biological advantages of culture media.

THE DIGITAL PETRI DISH: TAKING YOU INTO THE FUTURE

CULTURE of INNOVATION

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the dIgItal petrI dIsh: takIng you Into the future

50 yeArS Of InnOvATIOn In mIcrOBIOlOgy

With over a century of experience in biology and in vitro diagnostics,

expertise and innovation are a tradition in the Mérieux family.

Naturally, at bioMérieux, R&D is a primary strategic focus that we back

with considerable investment. Understanding and anticipating progress,

seizing opportunities and integrating new technologies are some of

the key objectives we set ourselves. We are dedicated to being your

partner of choice in the fight against infectious diseases.

innovation at biomérieux SInce 1963, IS

• hundreds of patents submitted in 166 files

• more than 10 new culture media products marketed within 2 years

• 10 r&D sites worldwide, with 3 entirely dedicated to culture media research

With chromID™, biomerieux offers you an extensive range of high medical-value chromogenic media for the simultaneous culture and identification of micro-organisms.

With PrevI™ Isola, biomérieux has taken automated sample inoculation to another level. Its innovative applicator uses high precision technology to standardize, to increase numbers of isolated colonies to optimize colony inoculation.

the range

making your daily life easier

streaks ahead: revolutionize your WorkfloW

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chromogenic media for the fight against Healthcare Associated Infections (HAI)

chromogenic media for Prevention of perinatal group B Streptococcal diseases

chromogenic media for detection of pathogens in cystic fibrosis patients

chromogenic media for direct identification

Blood Agar: complex, Selective and Differential media

Specific media: Selective Isolation for Specific Organisms

Simple media: Isolation of Organisms

media for Susceptibility Testing - etest strips

media for environmental controls: Surfaces, Air, Water

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PrODuct INSIGHT

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With chromIDTM bioMérieux offers you an extensive range of chromogenic media for the simultaneous culture and identifi cation of micro-organisms.

Designed to meet your increasing daily challenges, these high quality media provide you with reliable results and true peace-of-mind.

Our recognised expertise in culture, identifi cation and susceptiblity testing naturally led us to pioneer the chromogenic media concept back in 1989. Since then, we have continued to create innovative chromogenic solutions to meet your everyday needs.

an extensive range of chromogenic media

for the simultaneous culture and identifi cation

of micro-organisms

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chromID CARBAIncubation:18-24hours

K. pneumoniae ATCC®*BAA1705

C H R O M O G E N I C M E D I A F O R T H E F I G H T A G A I N S T H A I

chromID™ CARBAFor the screening of Carbapenemase producing Enterobacteria (cPe)

chromID™CARBAagarisaselectivechromogenicmediumforthescreeningofCarbapenemase-ProducingEnterobacteriaceae(CPE)inpatientswhoarechroniccarriersorinpatientsatrisk.

chromIDCARBAagarconsistsofanutrientbasecombiningdifferentpeptones,threechromogenicsubstrateswhichenablethedetectionofactivitiesofspecificmetabolicenzymesforE. coli,Klebsiella/Enterobacter/Serratia/CitrobacterandProteae,andaproprietarymixtureofantibioticsfavouringtheselectivegrowthofcarbapenemase-producingEnterobacteriaceae.

CPEareparticularlymulti-resistantbacteriathatarecapableofcausingnosocomialinfectionsandhospitalepidemics.ThedetectionofCPEcarriersisimportantforthepreventionandepidemiologicalmonitoringoftheseinfections.Inthiscontext,theuseofchromID™CARBAagarcontributestotheactivesurveillanceofCPE.

chromID™ CARBA agar

Ref. 43 861 • kit of 20 plates

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chromID™ c. diffi cile agarDirect Identifi cation of Clostridium diffi cile in 24 hours

chromIDC. diffi cileagarisaselectivechromogenicmediumforthedetectionandidentificationof Clostridium diffi cileinstoolsofsymptomaticpatients.

ThemediumcontributestothediagnosisandepidemiologicalmonitoringofClostridium diffi cile infections.

Clostridium diffi cileisacausativeagentofpseudomembranouscolitisandmoregenerallyofnosocomialorantibiotic-associateddiarrhea.

chromIDC.difficileagarconsistsofarichnutritivebasecombiningdifferentpeptones,taurocholatewhichfavorsthegerminationofsporesandachromogenicsubstrate(patented)whichenablethedetectionofspecificmetabolicenzymesforClostridium diffi cile,andamixtureofantibioticsfortheinhibitionofmostGram-positiveandGram-negativebacteria,yeastsandmolds.

chromID c. diffi cile agar


chromID c. diffi cile agarIncubation:24hours

C. diffi cile ATCC®*700057

Incubation:48hoursC. diffi cile ATCC®*9689zoomx2

clostridium diffi cile agar


E. coli 1011230zoomx2

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chromID™ MRSAfor the direct screening of methicillin-resistant S. aureus (mrSA) in 18-24 hours

chromIDMRSAisdedicatedtothesurveillancecultureorscreeningofMRSAtoidentifyhospitalizedpatientsrequiringisolation.ThescreeningofMRSAcarrierswithsurveillanceculturesisakeystepinthefightagainstnosocomialinfections.chromIDMRSAhasbeen

developedtoanswerthismajorhealthconcern.

IdentificationofMRSAstrainsisbasedonthespontaneousgreencolourationofα-glucosidase-producingcoloniesandthepresenceofamixtureofantibiotic.

chromID MRSA

Ref. 43 472 • kit of 20 platesRef. 43 479 • kit of 100 plates

Ref. 43 451 • kit of 20 platesRef. 43 459 • kit of 100 platesRef. 43 841 • kit of 20 plates (1)

Enrichment broth

Brain-Heart brothRef. 42 081 • kit of 20 tubes

Todd-Hewitt broth + antibioticsRef. 42 116 • kit of 20 tubes

Agglutination Tests

Slidex mrSA DetectionRef. 73 117 • kit of 50 tests

(1) For USA only FDA approved

chromID MRSAIncubation:18hoursS. aureus ATCC®*43300Ref.43472

MRSAfromanasalswabspecimen.

ImageprovidedbySullivan&NicolaidesPathology,Brisbane,Australia-Ref.43451

chromID™ ESBLfor the screening of extended-Spectrum ß-lactamase producingEnterobacteria (eSBl)

chromID ESBL agar contains:•Amixtureofantibiotics,includingcefpodoxime,

enablingtheselectivegrowthofESBL-producingenterobacteria(patented).

•TwochromogenicsubstratesandonenaturalsubstrateenablingthedirectidentificationofthemostfrequentlyencounteredESBL-producingenterobacteria(patented).

-Escherichia coli:spontaneouscoloration(pinktoburgundy)ofß-glucuronidase-producingstrains.

-Klebsiella, Enterobacter, Serratia, Citrobacter: spontaneousgreen,brownish-greenorbluecolourationofstrainsexpressingaß-glucosidase.

-Proteeae(Proteus, Providencia, Morganella):spontaneousdarkbrowntolightbrowncolourationofstrainsexpressingadeaminase.

Whereasidentificationtothespeciesorgrouplevelisdirect,ESBLproductionmustbeconfirmedbyadditionaltests.

chromID ESBL agar


K. pneumoniae ATCC®*700603

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E. coli CIP103982

chromID ESBLIncubation:18-24hours

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chromID MRSA / chromID S.aureus

Incubation:24hoursS. aureus ATCC®*43300

chromID MRSA /chromID S.aureus

Ref. 43 466 • kit of 20 biplates

chromID™ MRSA / chromID™ S.aureusfor a better follow-up of S.aureus epidemiology

Chromogenicmediumforthescreeningofmethi-cillin-resistantStaphylococcus aureus(MRSA).ChromogenicmediumfortheselectiveisolationofstaphylococciandthedirectidentificationofS. aureus.

AssociationofchromIDMRSAandchromIDS.aureusforthedirectidentificationof S.aureus andMRSAforsurveillancecultureofS.aureusandMRSAfromnasal,axillary,inguinalorperitonealsamples.

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chromID VRE

Ref. 43 004 • kit of 20 platesRef. 43 851 • kit of 20 plates (1)

Brain-Heart infusion broth

Ref. 42 081 • kit of 20 tubes

chromID VRE Incubation:24hours

chromID™ VREFor the screening of E. faecium and E. faecalis showing acquired vancomycin resistance (vre)

chromIDVREcontainstwochromogenicsubstrates(α-Glucosidase&ß-Galactosidase)andVancomycin(8mg/l)whichenable:•DirectidentificationofE. faeciumandE. faecalis

after24hourincubation.•Specificandselectiveisolation&detectionof

acquiredVancomycin-Resistantenterococci(VanA,VanB).

•Characteristiccolourationofcolonieswith:Bluish-greencolour=E. faecalisVioletcolour=E. faecium

Theselectivemixtureinhibits:-enterococciwithnaturalresistance(ie.E.

casselifl avusandE. gallinarum…)-mostGram-negativeandGram-positivebacteria

aswellasyeasts.

E. faecalisATCC®*51299zoomx2

E. faecalisE. faeciumCCUG36804zoomx2


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C H R O M O G E N I C M E D I A F O R P R E V E N T I O N O F P E R I N A T A L G R O U P B S T R E P T O C O C C A L D I S E A S E S

granada™ / columbia cnA agar + 5% sheep bloodFor enhanced Streptococci epidemiology follow up

SelectivemediumforthescreeningandidentificationofgroupBstreptococci(S. agalactiae)/Selectiveisolationoffastidiousbacteria.Detectionofhemolysis.

TheGranadaagarallowsthedirectidentificationofGBSandtheColumbiaagarpartallowstheisolationofGBSnonhemolyticandGroupAStreptococciwhichmayberesponsibleofmeningitis,pneumonia,puerperalsepsis.

Incubationinanaerobicconditions.

Granada / columbia cnA agar + 5% sheep bloodIncubation:24hours

chromID Strepto BIncubation:24hoursS. agalactiae ATCC®*12386

chromID Strepto B


SlIDeX Strepto Plus B

Ref. 58 819 • kit of 50 latex tests

granada / columbia cnA agar + 5% sheep blood


Genbag / Genbox

See page 37

SlIDeX Strepto Plus

Ref. 58 811 • kit of 50 latex tests

EnterococcusS. agalactiaefromvaginal

specimenszoomx2

S. agalactiaeATCC®*12386

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chromID™ Strepto Bfor the screening of all S. agalactiae in pregnant women

•Threechromogenicsubstratestooptimisetheidentificationofallgr.BStreptococcus=palepinktoredcolonieswhichareroundandpearlyafter18-24hourincubation.

•ExcellentperformancefortheGBSprenatalscreeningintermsofnutrientcapacityandsensitivityofdetection.

•DetectionofallGBSstrains,includingnonß-hemoliticstrains.

•Differentiationofmixedcultures.•Selectiveinhibitionofmostbacterianot

belongingtothespeciesS. agalactiae,aswellasyeasts.

Incubationinaerobicconditions.

Todd-Hewitt broth + antibiotics

Ref. 42 116 • Kit of 20 x 9 ml tubes

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S. agalactiae ATCC®*12386zoomx2

Granada Biphasic Broth


API® suspension MEDIUM

Ref. 70 640 • kit of 100 ampoules (3 ml)

PSIpettes

Ref. 70 250 • kit of 400 units (5 ml)

Granada Biphasic brothSelective broth for the screening and direct identifi cation of S. agalactiae

granada biphasic broth enables in a single test: Direct specimen inoculation - Transportation - enrichment - culture - Identifi cation Highly economical:itprovidessignificanttimeandworkloadsavings

Total flexibility:•thetubecanbeusedbothinlabsorfor

delocalisedpatientsamplingandinoculationfollowedbyimmediateincubationofGBSwithin18-24hours

•storage:atroomtemperature•incubationtakesplaceinanaerobicatmosphere

Granada™ agarSelective agar for the screening and direct identifi cation of S. agalactiae

GranadaagarisaselectivemediumforthescreeninganddirectidentificationofStreptococcus agalactiaecarriageinpregnantwomenandnewbornsusingclinicalspecimens

(anovaginalandurinesamplesfrompregnantwomen,oringestedgastricfluidfromnewborns).ThemediumwasfirstdescribedbyDrDeLaRosaandal.

Incubationinanaerobicconditions.

Granada Biphasic broth Incubation:18-24hours

S. agalactiae ATCC®*12386

Granada agar


Todd-Hewitt broth + antibiotics

Ref. 42 116 • Kit of 20 x 9 ml tubes

Granada agarIncubation:18-24hours

C H R O M O G E N I C M E D I A F O R P R E V E N T I O N O F P E R I N A T A L G R O U P B S T R E P T O C O C C A L D I S E A S E S

Genbag / Genbox

See page 37

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P. aeruginosa morphotypes

incysticfibrosisspecimens

S. aureus (greencolonies),S. saprophyticus (pinkcolonies),

S. xylosus (mauvecolonies)

Visualisationofgolden-metallic

colouredP. aeruginosa

colonieszoomx3

S. epidermidis (whitecolonies)

chromID P. aeruginosaIncubation:24hoursP. aeruginosa ATCC®*27853

chromID S. aureusIncubation:24hoursS. aureus ATCC®*25923

C H R O M O G E N I C M E D I A F O R D E T E C T I O N O F P A T H O G E N S I N C Y S T I C F I B R O S I S P A T I E N T S

chromID™ P. aeruginosafor the direct identifi cation of Pseudomonas aeruginosa

ThedirectidentificationofP. aeruginosaisbasedonthespecificpinktovioletcolorationofaminopeptidase-producingcoloniesduetothechromogenicsubstrateß-alanyl-resorufamine(2bioMérieuxpatents).

chromID™ S. aureusfor the the direct identifi cation of S. aureus

DirectidentificationofS. aureusisbasedonthespontaneousgreencolourationofglucosidase-producingcolonies(patentpending).

RapiditywiththeimmediateidentificationofS. aureus=Greencolonies(readingbetween18and24hours).

•ExcellentperformanceforthecultureofS. aureusintermsofnutrientcapacity,detectionsensitivityandcolourationspecificity.

•Optimumdifferentiationofmixedculturesduetothepresenceofa2ndsubstrate.

•OrientationofidentificationtowardsStaphylococci=S. epidermidis(whitecolonies),S. saprophyticus(pinkcolonies),S. xylosus (mauvecolonies).

Inhibitionofotherbacteria(Gram+andGram-)andyeasts.

chromID P. aeruginosa


chromID S. aureus


chromID MRSA / chromID S. aureus

Ref. 43 466 • kit of 20 tests

Slidex® MRSA

Ref. 73 117 • kit of 50 tests

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BCSA agar


BCSA agarSelective isolation of Burkholderia cepacia

BCSAagar(Burkholderia cepaciaSelectiveAgar)isamediumrecommendedfortheselectiveisolationanddetectionofthespeciesBurkholderia cepaciacomplexefromclinicalspecimensofmainlyrespiratoryorigin(sputumandbronchoalveolarwashing)inpatientssufferingfromcysticfibrosis.

Thepresenceofapeptonebaseandsugarsenableoptimumgrowthofthemicro-organisms.

Crystalvioletandtheantibioticspresentinthemediuminhibitmostmicrobialspecies(inhibitionofPseudomonasspecies).

Ahighvolumeofagarperplateenablesgoodperformanceforcultureat72hours.

BCSA agar Incubation:48hours

B. cepacia ATCC®*25416

M E D I A F O R D E T E C T I O N O F P A T H O G E N S I N C Y S T I C F I B R O S I S P A T I E N T S

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Other culture mediafor the recovery of other micro-organisms involved

columbia agar + 5 % sheep bloodfortherecoveryofStreptococcussppincludingStreptococcus pneumoniae

chocolate agar + PolyviteX™fortherecoveryofHaemophilusandothersfastidiousorganisms

mac conkey agarfortherecoveryofEnterobacteriaceae

columbia agar + 5% sheep blood

Incubation:24hoursS. pneumoniae ATCC®*6305

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K. pneumoniaeEnterococci

zoomx2

S. saprophyticuszoomx2

chromID cPS / columbia cnA + 5% sheep blood

Ref. 411 617 • kit of 20 biplatesRef. 43 473 • kit of 20 biplates

chromID™ CPS® / columbia cnA+ 5% sheep bloodChromogenicmediumfortheenumerationoforganismsinurinespecimensandthedirectidentificationofEscherichia coli,Enterococcus,KESCandProteeae.

Selectiveisolationoffastidiousbacteria.

Determinationofhemolysis.

chromID CPS / columbia cnA + 5% sheep bloodIncubation:24hoursEnterococcus faecalis ATCC®*29212

chromID CPSIncubation:18-24hoursE.coli ATCC®*25922•Proteus mirabilis ATCC®*12453andEnterococci ATCC®*29212

C H R O M O G E N I C M E D I A F O R D I R E C T I D E N T I F I C A T I O N

chromID™CPS®

for the isolation, enumeration and direct identifi cation of E. coli, Proteus and Enterococci and KeSc in one single step using urine specimens

Isolationandenumerationofallurinarytractpathogens.Coloniesarewellisolatedandeasytoidentifywithrealdifferentiatingcolours.Microbialenumerationwithcolourlessbackgroundtooptimisecolonycounting.

Direct identifi cation:chromIDCPScontainsspecificsubstratesoftheenzymaticactivitiestobedetected.

Ease-of-use: •E. coli:Noindoletestrequired.•Proteus:spontaneouscolourationofcolonies

producingdeaminase.•Enterococci.

reliability: •Nutrientcapacity.•Sensitivityofdetectionandspecificityof

colourationforthemainorganismsusuallyfoundinurine.

•IdentificationofthebacterialgroupKESC.

Storage: shelf-life includes 1 week storage at 15°-25°c.

chromID CPS agar


zoomx2

23

chromID candida / Sgc2


chromID™ Candida / Sabouraud gentamicin chloramphenicol 2for greater visualisation of colonies

SelectiveisolationofyeastsanddirectidentificationofCandida albicans.

Selectiveisolationofyeastsandmould.

chromID™ Candidafor the selective isolation of yeasts and the direct identifi cation of Candida albicans in 24 hours

Candida albicanscoloniesarecolouredbluebythespecifichydrolysisofahexosaminidasechromogenicsubstrate(bioMérieuxpatent).

Thehydrolysisofasecondsubstrate(pinkcolouration)differentiatesmixedculturesandorientsidentificationofotherspeciescolonies(bioMérieuxpatent).

ImmediateidentificationofC. albicansinjust24hours*=BluecoloniesGreaterintensityforC. albicanscolonies.

•Optimumdifferentiationofmixedcultures•OrientationofidentificationtowardsCandida

(C. tropicalis, C. lusitaniaeandC. kefyr)=pinkcolonies

•Whitecoloniescharacteristicaspect=orientationtowardsyeastsorfilamentousfungi

chromID Candida / Sabouraud Gentamicin

chloramphenicol 2 Incubation:48hours

C. albicans ATCC®*10231

chromID Candida agar


chromID CandidaIncubation:48hours

C. albicans ATCC®*10231,C. glabrata ATCC®*90030,C.tropicalis ATCC®*9968


24hoursC. albicans ATCC®*10231C. krusei ATCC®*6250

zoomx2

Aspergillus nigerzoomx2

24

S. enteritidis, P. vulgaris

zoomx2

chromID Salmonella / Hektoen


chromID™ Salmonella / Hektoenfor simultaneous detection of Salmonella and Shigella

ChromogenicmediumfortheselectiveisolationanddifferentiationofthegenusSalmonella.SelectiveisolationofSalmonellaandShigella.

chromID Salmonella / HektoenIncubation:24hoursS. typhimurium ATCC®*14028

chromID SalmonellaIncubation:24hoursS. typhimurium ATCC®*14028


chromID™ Salmonellafor the selective isolation of all Salmonella serotypes

ThreechromogenicsubstratesoptimizetheselectiveisolationanddifferentiationofSalmonella:•Specificdetectionoftheesteraseenzymatic

activityonacolourlessbackground=Pale-pinktomauvecoloniesforSalmonella(readingbetween18to24hrs)

•Differentiationofotherbacteria(coloniesofadifferentcolour)

•InhibitionofGram-positivebacteriaandyeasts.

Thenutrientcapacity,colourintensity,sensitivityofdetectionandspecificityofcolouration,ensureoptimumdifferentiationofmixedcultures.

chromIDSalmonellaenablesthedetectionofserotypeTyphi,ParatyphiandmostSalmonella Lactose+.

chromID Salmonella agar


Selenite f broth


Rappaport broth


25

chromID Vibrio agar


chromID™ O157:H7for the selective isolation and presumptive identifi cation of E. coli O157:H7

ChromIDO157:H7,aninnovativechromogenicmediumenablingeasydifferentiationbetweentheO157:H7serotypeandothermicro-organisms:

•identificationofE. coliO157:H7afteronly24hrsofincubationat37°C

•characteristicgreentoblue-greencolourofE. coliO157:H7colonies

•morefertilethanSMACmedium,andequallyselective

•inhibitionofGram-positivebacteriaandyeasts

•increaseinselectivitywithrespecttoGram-negativebacteriabyaddingCefiximeTellurite,forhighlycontaminatedsamples.

chromID™ Vibriofor the selective isolation of Vibrio

PresumptiveidentificationofV. choleraeisbasedontheblue-greencolourationofbetaGalactosidase-producingcolonies.

DifferentiationofV. parahaemolyticus:Pinkcoloniesbasedonarabinoseassimilation.

chromID O157:H7Incubation:24hours

E. coli O157:H7ATCC®*43890Citrobacter freundii ATCC®*8090

E. coli ATCC®*11229Proteus mirabilis ATCC®*12453

chromID O157:H7

Ref. 42 605 • 6 x 200 ml bottles

cefi xime tellurite mixture

Ref. 42 606 • 6 x 4 ml (lyo)


Vibrio parahaemolyticusIncubation:24hours

E. coli O157:H7zoomx2

Fecalculturenaturallycontaminatedby

V. cholerae.ImagesfromDrScheftel,InstitutdeBactériologie,

Strasbourg,France.zoomx2

CitrobacterProteusOtherE. coliE. coliO157:H7

zoomx2

chromID Vibrio Incubation:24hours

V. cholera ATCC®*14035V. parahaemolyticus ATCC®*17802

26

The culture media are controlled at each stage of production: from medium design through to manufacturing with perfect traceability.

By means of rigourous raw material selection, signifi cant control of production and shelf-life validation according to a procedure including thermal shocks, performance and robustness are guarantee batch to batch.

Blood agar (complex, selective

and differential media), specifi c

media (selective isolation for

specifi c organisms) and simple

media (isolation of organisms)

complete the culture media

range beside chromid range.

Culture media

27

columbia cnA agar + 5% sheep blood


columbia cnA agar + 5% sheep bloodSelective isolation of fastidious bacteriaDetection of hemolysis

ColumbiaCNAagar+5%sheepbloodisaselectiveisolationmediumwhichenablesthegrowthofGram-positivebacteriacommonlyencounteredinclinicalspecimens.

MostGram-negativebacteriaandBacillusareinhibitedbythenalidixicacidandcolimycinintheagar.

columbia agar + 5% sheep bloodIsolation of fastidious bacteriaDetection of hemolysis

Columbiaagarisanisolationmediumspeciallydesignedtofacilitatethegrowthoffastidiousmicro-organisms.

Supplementedwithsheepblood,itishighlynutritiousandthereforeadaptedtothecultureofmostbacterialspecies,regardlessoftheirmetabolism.

columbia cnA agar + 5% sheep blood




B L O O D A G A R : C O M P L E X , S E L E C T I V E A N D D I F F E R E N T I A L M E D I A

Incubation:24hoursS. pyogenes ATCC®*19615

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Incubation:48hoursS. aureus ATCC®*25923

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28

S. pyogenes ATCC®*19615

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S. pyogenes ATCC®*19615


Trypcase Soy Agar + 5% sheep bloodIsolation of bacteria Determination of hemolysis

Trypcasesoyagar+5%sheepbloodisanisolationmediumwhichfavoursthegrowthofallthespeciescurrentlyfoundinclinicalspecimens.

Trypcase Soy Agar + 5% sheep bloodIncubation:24hoursS. pneumoniae ATCC®*63605

columbia agar + 5% horse bloodIsolation of fastidious bacteria Detection of hemolysis

Columbiaagar+5%horsebloodisanisolationmediumwhichhasbeendevelopedtofacilitatethegrowthoffastidiousmicro-organisms.

columbia agar + 5% horse blood


columbia agar + 5% horse bloodIncubation:24hoursS. pneumoniae ATCC®*6305

Trypcase soy agar + 5% sheep blood


29

chocolate agar + PolyviteX™ vcAT3 agarSelective isolation of Neisseria gonorrhoeae & Neisseria meningitidis

Chocolateagar+PolyViteXVCAT3agarisaselectivemediumfortheisolationofNeisseria gonorrhoeae&Neisseria meningitidis frompolymicrobialspecimens(urogenital,oropharyngeal,CSF,bloodcultures...).

ThemediumiscomposedofanutrientbaseenrichedwithfactorsX(hemin)andV(NAD)providedbyhemoglobinandPolyViteX.Theenhancedselectivityofthemediumisobtainedbythecombinationofantimicrobialandantifungalagents.


chocolate agar + PolyviteX™Isolation of fastidious bacteria

Chocolateagar+PolyViteXisanisolationmediumparticularlyrecommendedforthegrowthoffastidiousstrainsbelongingtothegeneraNeisseria,Haemophilus,andStreptococcus pneumoniaeencounteredinclinicalspecimens.

ThismediumiscomposedofanutrientbaseenrichedwithfactorsX(hemin)andV(NAD)providedbythehemoglobinandPolyViteX.

Thismediumcanbeusedtosubculturebacterialstrainsinordertoobtainpurecultures.


chocolate agar + PolyviteX vcAT3 agar

Incubation:24hoursN. gonorrhoeae ATCC®*43069

chocolate agar + PolyviteX™ vcAT3 agar


chocolate agar + PolyviteXIncubation:48hours

H. infl uenzae ATCC®*10211


chocolate agar + PolyviteX


Incubation:24hoursN. meningitidis ATCC®*13090

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Incubation:48hoursN. gonorrhoeae ATCC®*43069,S. pneumoniae

ATCC®*6305zoomx2

30

Haemophilus chocolate 2 agarIncubation:24hoursH. infl uenzae ATCC®*10211


Haemophilus chocolate 2 agarSelective isolation of Haemophilus

HaemophilusChocolate2agarisaselectivemediumfortheisolationofthedifferentHaemophilusspeciesfrompolymicrobialspecimensofhumanorigin.

ThisnewformulaiscomposedofanutritivebaseenrichedwithfactorsX(hemin)andV(NAD)providedbyhemoglobinandPolyViteX™.

IsolationofHaemophilusspeciescollectedfromtherespiratoryorgenitaltractsisoftendifficultduetothepresenceoflargeamountsofassociatedflora.

Theselectivityofthemediaisobtainedbythecombinationofantimicrobialandantifungalagents.

Haemophiluschocolate 2 agar


31

Sabouraud Gentamicin chloramphenicol 2 agarSelective isolation of yeasts and moulds

SabouraudGentamicinChloramphenicol2agarisaselectivemediumrecommendedfortheisolationofyeastsandmouldsfrompolymicrobialspecimens.

Thenewformulahasincreasednutrientcapacity,colourintensity,sensitivityofdetectionandspecificityofcolourationforfungi.Theincreasedlevelofpeptonesanddextrosefavoursthegrowthoffungalstrains.ThepresenceofgentamicininhibitsmostGram-negativeandGram-positivebacteria.Thepresenceofchloramphenicolimprovestheselectivityforcertainspecieswhichmayberesistanttogentamicin(streptococci, Proteus,etc.).ThepHoftheagarwhichisslightlyacidic,favoursfungalgrowthmorethanbacterialgrowth.

Incubation:72hoursAspergillus niger ATCC®*16404

Sabouraud Gentamicin chloramphenicol 2 agar


Dermatophyte agar


Sabouraud Gentamicin chloramphenicol 2 agar

Incubation:24hoursC. albicans ATCC®*10231

S P E C I F I C M E D I A : S E L E C T I V E I S O L A T I O N F O R S P E C I F I C O R G A N I S M S

Incubation:72hoursAspergillus fl avuszoomx2

Incubation:72hoursAspergillus nidulanszoomx2

chromID candida / Sgc2


3232

A7 mycoplasma agar


Urea-Arginine lyO 2

Ref. 42 508 • kit for 25 tests

mycoplasma IST2

Ref. 42 505 • kit for 25 tests

mycoplasma preparationRef. 42 507 • 8 x 3.1 ml

A7 mycoplasma agarSelective culture and enumeration of urogenital mycoplasma

A7Mycoplasmaagarisaselectivemediumforthecultureofmycoplasmas,Ureaplasma spp.andMycoplasma hominisfromurogenitalspecimens.TheUreaplasma urealyticumspecieshasbeenseparatedintotwonewspeciescalledUreaplasma parvumandUreaplasma urealyticum.

Fortheuseofthismedium,theyshouldbothbeconsideredasUreaplasmaspp.Thismediumcombinesarichnutritivebasecontainingpeptones,horseserumandgrowthfactorsfavouringthegrowthofmycoplasmas(cysteine,PolyViteX™,arginine,urea).

•Ureaplasmaspp.“Seaurchin”colonies:roundcoloniessurroundedbyabrownish-blackprecipitate.

•Mycoplasma hominis“Friedegg”colonies:roundcolonieswithaprotrudingcentersurroundedbyalighterhalo.

AnantibioticmixtureinhibitsthegrowthofmostGram-positiveandGram-negativebacteria.

A7 mycoplasma agar

campylosel agarIncubation:48hoursCampylobacter jejuni ATCC®*33291


campylosel agarSelective isolation of Campylobacter

CampyloselagarisaselectivemediumfortheisolationofintestinalCampylobacter(C. jejuniandC. coliprincipally)fromstools.

Thepresenceofsheepbloodfavoursthegrowthofthetargetspecies.

Thenutrientcapacityisenhancedbytheuseofreducingagents.

Goodselectivityisensuredbytheantibioticandantifungalagentspresentinthemediumwhichinhibitthemajorityofbacterialandfungalcontaminants.

CharacteristicCampylobactercoloniesaresmallandgreyish,andsometimesspreadalongtheinoculationstreaks.

campylosel agar


Mycoplasma hominis

INVERTEDMICROSCOPE(OBJECTIVEX10)

Ureaplasma urealyticum

Genbag / Genbox

See page 37

3333

Cetrimide agar


SMAC CT agar Selective isolation of Escherichia coli O157:H7

TheSMACCTagar(MacConkeywithSorbitol)enablesthedetectionanddifferentiationofenterohemorrhagicEscherichia coliofserotypeO157:H7,responsibleforgastro-intestinalinfectionsordiseases.

Accordingtoepidemiologicaldata,thesetypesofpathologymayalsobeinducedbytheO157:H-serotype(non-motilebacteriaunliketheO157:H7serotype).

ThepresenceofsorbitolandtelluriteenablesthedifferentiationofE. coliO157:H7whichischaracterizedbycolourlesscolonieswithabrowncentre.E. coli0157:H7strainsmightalsogeneratecolourlesscolonieswithabrowncentre.

TheotherE. colifermentingsorbitolgivepinktoredcolonies.

TheselectivityforGram-positivebacteriaandcertainEnterobacteriaceaeisensuredbycefixime,bilesalts,crystalvioletandtellurite.

Cetrimide agarSelective isolation of Pseudomonas aeruginosa

CetrimideagarisaselectiveisolationmediumforthedetectionofPseudomonas aeruginosafromspecimensofvariousorigin.

CetrimideagarstimulatestheproductionofpyocyaninandthefluorescenceofP. aeruginosa.

ItsformulaisanoptimizationofKing’sAmedium:theadditionofaquaternaryammoniuminhibitsmostothermicro-organisms.

Characteristiccoloniespresentaspontaneouspalegreenpigmentationandgreenfluorescenceunderultravioletlight.

SMAC CT agarIncubation:24hours

E. coliO157:H7ATCC®*43894

SMAC CT agar


Cetrimide agar Incubation:48hours

P. aeruginosa ATCC®*27853


P. aeruginosa ATCC®*10145,UVlight

34 legionella gvPc agarSelective isolation of Legionella

LegionellaGVPCagarhasbeenspeciallydevelopedfortheisolationfromrespiratoryspecimensofmostLegionellaspecies,notablythoseresponsibleforinfections:Legionella pneumophila,whichisthespeciesmostfrequentlyinvolved(Pontiacfever)butalsoL. bozemanii, L. longbeachae, L. dumoffi i, L. jordanis, L. gormanii, L. anisaand L. micdadei.

ThisselectivemediumalsoenablestheenumerationofLegionellainwateraccordingtostandardsT90-431andISO11731.

TheactivatedcharcoalabsorbsanytoxicsubstancesintheyeastextractandstimulatesthegrowthofLegionella.

TheACESbufferstabilizesthepHofthemediumat6.9,whichistheoptimalpHforgrowth.

ThecharacteristiccoloniesofLegionellaaregrey-blueincolourbutwhichcanbecomeoff-whitewithage.

Theyhavearegularpinkishedgeand,whenobservedunderabinocularmicroscope,haveagroundglassappearance.

GVPCmediumprovidesexcellentselectivitythroughanoptimizedcombinationofthreeantibiotics.

legionella gvPc agarIncubation:5daysLegionella pneumophila

D-coccosel agarIncubation:24hoursE. faecalis ATCC®*29212


D-coccosel agarSelective isolation of enterococci and group D streptococci

D-Coccoselagar(BileEsculinAgar)isusedfortheselectiveisolationanddifferentiationofenterococciandgroupDstreptococcifrompolymicrobialspecimens.

ThecharacteristiccoloniesofenterococciandgroupDstreptococciarecolourlessorgreyandsurroundedbyablackhalo(hydrolysisofesculin).

TheselectivityofthemediumforGram-negativebacteriaisprovidedbysodiumazide.BileinhibitscertainGram-positivebacteriaotherthanenterococci.

Legionella pneumophila

zoomx2.5

zoomx2

D-coccosel agar


legionella gvPc agar


Slidex® legionella Kit

Ref. 73 120 • kit of 50 tests(not ce marked)

35

Yersinia CIN agarSelective isolation of Yersinia

YersiniaCINagarisaselectiveisolationmediumforthedetectionanddifferentiationofYersinia speciesfromstoolspecimens.

TheformulaofthemediumisthatdescribedbySchiemann(CINmedium:Cefsulodin,Irgasan,Novobiocin).

ThemannitolandneutralredpresentinthemediumenableYersiniatobedifferentiatedbythecolourofthecolonies(darkpinktoredcolonies).

Thepresenceofcholate,deoxycholate,crystalviolet,IrgasanandantibioticsinhibitsthegrowthofGram-positiveandmostGram-negativebacteria.

gardnerella agarSelective isolation of Gardnerella vaginalis

GardnerellaagarisaselectiveisolationmediumforthedetectionofGardnerella vaginalisingenitalspecimens.

Gardnerella vaginalis,aloneorassociatedwithcertainanaerobicbacteria(Mobiluncus,BacteroidesandPrevotella),isresponsibleforvariousurogenitalinfections.

Thepresenceofhumanbloodfavoursthegrowthofthespeciesbeingtestedforandproducesß-hemolysisaroundthecolonies.ß-hemolysis

obtainedsolelyonhumanbloodagarishighlyindicativeofG. vaginalis.

TheantibioticsincludedinthemediuminhibitmostGram-negativecontaminantsandyeasts.

Yersinia CIN agar Incubation:24hours

Y. enterocolitica ATCC®*9610

gardnerella agarIncubation:24hours

G. vaginalis ATCC®*14018


Incubation:48hoursY. enterocolitica ATCC®*9610

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zoomx2

gardnerella agar


Yersinia CIN agar


36

Pylori agarSelective isolation of Helicobacter pylori

PyloriagarisaselectiveisolationmediumforthedetectionofHelicobacter pyloriingastricbiopsies.

Helicobacter pyloriisacauseofgastritisandisassociatedwiththedevelopmentofgastricandduodenalulcers.

Helicobacter pyloriisamicroaerophilicbacteriumwhoseviabilityisreducedoncontactwithair.

Thesecharacteristicsshouldbetakenintoaccountfortransportandculture.

ThepresenceofhorseplasmaandPolyViteXenhancesthegrowthofthetargetspecies.

Theantibioticspresentinthemediuminhibitthemajorityofbacterialcontaminants.

Pylori agarIncubation:48hoursH. pylori ATCC®*43504


H. pylori ATCC®*43504

zoomx2.5

Pylori agar


Portagerm pylori


clostridium diffi cile agarSelective isolation of Clostridium diffi cile

Clostridium diffi cileagarisaselectiveisolationmediumwhichisspeciallydevelopedforthegrowthofClostridium diffi cileinstoolspecimens.Clostridium diffi cileisacausativeagentofPseudo-membranouscolitisandmoregenerallyofantibiotic-associateddiarrhea.

Thepresenceofsheepbloodfacilitatesthegrowthofthespeciesbeingtestedfor.Thecharacteristiccoloniesaregreyandsometimesspreadalongtheinoculationstreaks.Theantimicrobialandantifungalagentsinthemediuminhibitmostbacterialandfungalcontaminants.



clostridium diffi cile agarIncubation:48hoursC. diffi cile ATCC®*9689

zoomx2

Genbag / Genbox

See page 37


37

genbag / genbox…Atmosphere generators and incubation jars

Schaedler agar + 5% sheep bloodIsolation of anaerobic bacteria

Schaedleragar+5%sheepbloodisanisolationmediumparticularlysuitableforthedetectionofobligateandfacultativeanaerobicbacteria.

Thepresenceofgrowthfactorssuchasyeastextract,heminandvitaminK3andtheadditionofsheepblood,enablethegrowthofevermostfastidiousspecies.

Thereducingagent(L-cystine)andhighconcentrationofdextroseintheagarfavourthegrowthofanaerobicspecies.

Schaedler agar + 5% sheep blood

Incubation:48hoursB. fragilis ATCC®*25285


Incubation:72hoursVeillonella parvula ATCC®*10790

Incubation:24hoursC. perfringens ATCC®*13124Schaedler agar

+ 5% sheep blood


Schaedler neo. vanco. + 5% sheep blood


GENbag

Ref. 45 511 • clip seals x 10Ref. 45 532 • microaer - 20 testsRef. 45 533 • cO2 - 20 testsRef. 45 534 • anaer - 20 testsRef. 96 118 • anaer indicator 50 strips

GENbox

Ref. 96 124 • anaer - 10 sachetsRef. 96 125 • microaer 10 sachetsRef. 96 126 • cO2 - 10 sachetsRef. 96 127 • Jar 2.5 litersRef. 96 128 • Jar 7.0 litersRef. 96 118 • anaer indicator 50 strips

38

S I M P L E M E D I A : I S O L A T I O N O F O R G A N I S M S

CLED agarIncubation:24hoursE.coli ATCC®*25922(yellowcolonies)&P. mirabilis ATCC®*12453(colourlesscolonies)

BCP agarIncubation:24hoursE. coli ATCC®*25922(yellowcolonies)&P. vulgaris ATCC®*6380(colourlesscolonies)

CLED agarIsolation of urinary tract micro-organisms

CLEDagar(CystineLactoseElectrolyteDeficient)isespeciallyusefulfortheisolationofurinarytractmicro-organisms.Italsoenablesthedifferentiationoflactose-fermentingandnonlactose-fermentingbacteria(bromothymolblueindicator).•Lactose(+)bacteriaproducepaleyellowtoyellowcoloniesbyacidificationofthemedium.

•Non-lactosefermentingbacteriaproducegreen,blueorcolourlesscolonies.Thecompositionofthemedium(weakelectrolytecontent)preventstheswarmingofProteus.

ThenewCLEDformulaprovidesrealadvantagesintermsoflargercolonysize,improvementoflactosecharacterreading,andbettercolonydifferentiation.

BCP agarIsolation of common micro-organisms

BCPagar(LactoseagarwithBromcresolpurple)isanisolationanddifferentiationmediumforthedevelopmentofallorganismscommonlyfoundinspecimensofvariousorigin.

Italsodifferentiateslactose-fermentingorganismsfromnon-fermentingorganisms.Lactose(+)organismsproduceyellowcoloniesbyacidificationofthemedium.Non-fermentingorganismsproduceblueorcolourlesscolonies.

BCP agar


CLED Andrade agar


CLED agar


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39

Drigalski agar


Drigalski agarSelective isolation of Enterobacteriaceae and other Gram-negative bacteria

DrigalskiagarisaselectiveisolationanddifferentiationmediumusedtoidentifyEnterobacteriaceaeandotherGram-negativebacteriainfeces,urineorotherbiologicalspecimens.

Micro-organismsthatfermentlactoseformyelloworyellowish-greencolonies;theothersproduceblue,green,orbluish-greencolonies.

ThepresenceofsodiumdeoxycholateandcrystalvioletinhibitsthegrowthofGram-positive.


Drigalski agarIncubation:24hours

E. coli ATCC®*25922(yellowcolonies)&Salmonella typhimurium ATCC®*14028

(bluecolonies)

eosin-methylene blue agarIsolation of Enterobacteriaceae

Eosin-methyleneblueagarisaselectiveisolationanddifferentiationmediumforthedetectionofEnterobacteriaceae.

Itenableslactose-and/orsucrose-fermentingmicro-organismstobedifferentiatedfromnon-fermentingmicro-organisms.

Eosin-methyleneblueagarisusedtodetectenterobacteriainstools,urineorotherbiologicalspecimens.

Lactose(+)and/orsucrose(+)micro-organismsproducedarkpurplecoloniesbyacidificationofthemedium,whichmaybeaccompaniedbyametallicsheen.

Non-fermentingmicro-organismsproducecolourlessorslightlypinkishcolonies.

Thepresenceof2stainsinhibitsthegrowthofGram-positivebacteria.

eosin-methylene blue agar


eosin-methylene blue agarIncubation:24hoursE. coli ATCC®*25922

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40


Hektoen agar Incubation:24hoursSalmonella typhimurium ATCC®*14028

mac conkey agarIncubation:24hoursE. coli ATCC®*25922(pinkcolonies)&Salmonella cholerasuis st typhimuriumATCC®*14028

mac conkey agarSelective isolation of Enterobacteriaceae and Escherichia coli

MacConkeyagarwithcrystalvioletisaselectiveisolationanddifferentiationmediumforthedetectionofEnterobacteriaceaeinspecimensofvariousorigins.

Thismediumisspeciallydesignedtodetectthefermentationoflactosethroughacolourchangetoneutralred.•lactose(+)colonies:pinktored,sometimessurroundedbyahaloofprecipitatedbilesalts.•lactose(-)colonies:colourlessorslightlybeige.

TheselectivityforGram-positivebacteriaisprovidedbybilesaltsandcrystalviolet.


mac conkey agar


Hektoen agarSelective isolation of Salmonella and Shigella

HektoenagarisaselectiveisolationanddifferentiationmediumrecommendedforthedetectionofSalmonellaandShigellaspecies.

Themediumisinoculatedfromstoolspecimens,suspensionofstoolsoranenrichmentbroth.

Micro-organismsthatfermentoneofthethreesugarsinthemediumformyelloworyellowish-pinkcolonies;theothersproducegreentobluish-greencolonies.

H2S-producingmicro-organismsformcolonieswithablackcentre.•ColoniesofSalmonellaaregreentobluish-green,withorwithoutablackcentre.•ColoniesofShigellaaregreentobluish-greenwithoutablackcentre.

InhibitionofGram-positivemicro-organismsisobtainedbyamixtureofbilesaltsandstains.

Hektoen agar


Rappaport broth


Selenite f broth


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41


SS agarIncubation:24hours

Salmonella typhimurium ATCC®*14028(colonieswithblackcentre)&S. fl exneri ATCC®*12022

(palepinkcolonies)

Rappaport broth


Selenite f broth


SS agar


SS agarSelective isolation of Salmonella and Shigella

SSagarisaselectiveisolationanddifferentiationmediumrecommendedforthedetectionofSalmonellaandShigellaspecies.Themediumisinoculatedfromstoolspecimens,suspensionofstoolsoranenrichmentbroth.

Themediumdetectslactose-fermentingandthio-sulfate-reducing(productionofH2S)colonies.Lactose-fermentingmicro-organismsproducepinkcolonies,theothersgivecolourlesscolonies.

H2S-producingmicro-organismsproducecolonieswithablackcentre.•ColoniesofSalmonellaarecolourlesstopaleyellow,withorwithoutablackcentre.•ColoniesofShigellaarecolourlesstopalepinkororange-colouredwithoutablackcentre.

TheinhibitionofGram-positivemicro-organismsisobtainedbyamixtureofbilesaltsandstains.

mannitol Salt 2 agar Incubation:24hours

S. aureus ATCC®*25923mannitol Salt 2 agarSelective isolation of staphylococci

MannitolSalt2agarisintendedfortheselectiveisolationofstaphylococciinhumanspecimensandorientationofidentificationofS. aureus.

Thenewformulahasincreasednutrientcapacity,sensitivityofdetectionandspecificity.

Micro-organismswhichfermentmannitolproduceyellowcolonies.ThischaracteristicisacriterionfororientingidentificationofStaphylococcus aureus.ThehighcontentofsodiumchlorideinthemediumlimitsthegrowthofcertainbacteriaotherthanStaphylococcus.


mannitol Salt 2 agar


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42

mueller Hinton 2 agarIncubation:24hoursE. coli ATCC®*25922

M E D I A F O R S U S C E P T I B I L I T Y T E S T I N G

mueller Hinton 2 agar Susceptibility to antibiotics and sulfonamides

MuellerHinton2agarisamediumforantimicrobial susceptibilitytestingbydiscdiffusionandEtest strips.

ThecompositionofMuellerHinton2agarenablesthegrowthofnon-fastidiousbacteria(Enterobacteriaceae,nonfermentingGram-negativebacilli,staphylococciandenterococci)foundinhumanpathology,whileguaranteeingminimuminterferencefromtheconstituentsoftheformulawiththeresultoftheantimicrobialsusceptibilitytest.

TheconcentrationofdivalentionsintheagarisadjustedtoensureamoreaccuratedeterminationofthesusceptibilityofPseudomonas to aminoglycosidesandtetracyclines.

Thelowconcentrationofthymine–thymidine(sulfonamideinhibitors)restrictsthegrowtharoundthediscs,enablingmoreaccuratemeasurementofthezonesofinhibition.

TheuseofMuellerHinton2agarisincompliancewiththeCLSIandCA-SFMstandards.

mueller Hinton 2 agar

Ref. 43 301 • kit of 20 platesRef. 43 309 • kit of 100 platesRef. 43 302 • kit of 20 plates 145 mmRef. 43 511 • kit of 20 plates 120x120 mm

Brucella blood agar etest


RPMI agar Etest

Ref. AEB 122 180 • kit of 10 plates 90 mmRef. AEB 122 182 • kit of 10 plates 140 mm

43

mueller Hinton 2 agar + 5% sheep bloodSusceptibility of pneumococci and other streptococci to antibiotics and sulfonamides

MuellerHinton2agar+5%sheepbloodisamediumforantimicrobialsusceptibilitytestingbydiscdiffusionforstrainsrequiringbloodfortheirgrowth.

ThecompositionofMuellerHinton2agar,supplementedwithsheepblood,enablesthegrowthofbacteriasuchaspneumococciandotherstreptococci.

TheuseofMuellerHinton2agarisincompliancewiththeCLSIandCA-SFMstandardsandfullycompatiblewithEtest strips.

mueller Hinton 2 agar + 5% sheep blood


mueller Hinton 2 agar + 5% horse blood + nAD

Incubation:24hoursH. infl uenzae NTCC8468

M E D I A F O R S U S C E P T I B I L I T Y T E S T I N G

mueller Hinton 2 agar + 5% sheep blood

Ref. 43 321 • kit of 20 platesRef. 43 329 • kit of 100 platesRef. 43 324 • kit of 20 plates 120x120 mm

mueller Hinton 2 agar + 5% horse blood + nAD

Ref. 43 901 • kit of 20 platesRef. 43 919 • kit of 100 platesRef. 43 904 • kit of 20 plates 20x120x120 mm

mueller Hinton 2 agar + 5% horse blood + nADStudy of the antimicrobial susceptibility of pneumococci and other streptococci, Haemophilus and Moraxella

MuellerHintonagarwith5%horseblood+20mg/lβ-NADisamediumfordiscdiffusionandEtest stripsantimicrobialsusceptibilitytestingoffastidiousmicro-organisms(pneumococciandotherstreptococci,Haemophilus,andMoraxella).

Themediumhasbeendevelopedaccording to eUcAST (european committee on Antimicrobial Susceptibility Testing) recommendations.

44

Count-Tact® applicator

DEVICESair IDEAL® 3P™

M E D I A F O R E N V I R O N M E N T A L C O N T R O L S : S U R F A C E S , A I R , W A T E R

AerobiocollectorMeasuring aerobiocontaminationAir quality and surface controls are key processes in the Quality system of environmental control. air IDEAL® 3P™ ensures the effective measurement of aerobiocontamination in total confi dence with validated culture media.

ISO 14698 vAlIDATeDair IDEAL® 3P TRACEABILITY equippedwith90/100mmdiametersamplinggrids. . . . . . . . . . Ref.410175

air IDEAL® 3P TRACEABILITY equippedwith55/60mmdiametersamplinggrids. . . . . . . . . . . . Ref.410174

air IDEAL® 3P TRACEABILITY remotecontrol(RUID). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Ref.410173

Other devicesCount-Tact Applicator 1deviceforCount-Tactplates. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Ref.96300

Bi-Box 10sterilecasesforCount-Tactplatesforcollectionandtransport. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Ref.96301

Bi-Box 90 10sterilecasesfor90mmplatesforcollectionandtransport. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Ref.96311

Surface count-Tact™ plates ø 55 mmFor use in unprotected areasCount-Tact agar 20plates55mm. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Ref.43501

Count-Tact Sabouraud Dextrose Chloramphenicol agar 20plates55mm. . . . . . . . . . . . . . . . . . . . . . Ref.43580

Count-Tact chromID ESBL 20plates55mm. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Ref.411673

Count-Tact chromID MRSA 20plates55mm. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Ref.410155

for use in clean areas triple-wrapped irradiated mediaCount-Tact 3P Irradiated agar 20/100plates55mm. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Ref.43691/43699

Count-Tact 3P Irradiated Sabouraud Dextrose Chloramphenicol agar 20plates55mm. . . . . Ref.43812

45

bi-box™ and count-tact® plates

m e d i a f o r e n v i r o n m e n t a l c o n t r o l s : s u r f a c e s , a i r , W a t e r

Air & environment ø 90 mmfor use in unprotected areastrypcase soy agar CEmarked 20/100plates90mm. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Ref.43011/43019

sabouraud Dextrose chloramphenicol agar 20plates90mm. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Ref.43596

for use in unprotected areastrypcase soy 3P Irradiated agar with neutralizers and ß-lactamases 20plates90mm. . . . . . . Ref.43287

sabouraud Dextrose chloramphenicol Irradiated agar 20plates90mm. . . . . . . . . . . . . . . . . . . . . . . . Ref.43814

Waterculture media and an identifi cation test in compliance with the ISO 11731, nf T 90-431 and nf T 90-461 standards.

legionellaFORDETECTIONANDNUMERATION

gVPc agar 20plates. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Ref.43031

gVPc agar 100plates. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Ref.43032

for coNfirmatioN of thE Legionella genus Bcye with l-cysteine 10plates. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Ref.43013

Bcye without l-cysteine 10plates. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Ref.43023

for idENtificatioN of L.pneumophila aNd L.anisa

slIDeX® legionella kit 50tests. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Ref.73120

othersENUMERATIONOFTOTALVIABLEAEROBICMICRO-ORGANISMS

r2a agar 20plates. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Ref.43551

more products for environmental monitoring on

AeS chemunex catalogue

46

MICRO-ORGANISMS REF. NAME PLATE CLASSIFICATION SPECIMENS

All 43041/43049 Columbiaagar+5%sheepblood BloodagarUrine,Genital,Suppuration,Throat,Bloodculture,Cerebrospinal…

AllbacteriaGram+ 43071/43079 ColumbiaCNAagar+5%sheepblood Bloodagar

Urine,Genital,Suppuration,Throat,Sputum,Bloodculture,Cerebrospinal…

All 43050/43059 Columbiaagar+5%horseblood BloodagarUrine,Genital,Suppuration,Throat,Bloodculture,Cerebrospinal…

All 43001/43009 TrypcaseSoyAgar+5%sheepblood Bloodagar Genital,Throat,Bloodculture,

Cerebrospinal…

Anaerobes,Corynebacteria, Listeria, Helicobacter Pylori, Gardnerella 43401/43279 Schaedleragar+5%sheepblood Specificmedia Alltypesofspecimens

All + Branhamella, Pasteurella, Neis-seria, Haemophilus, Streptococci 43101/43109 Chocolateagar+PolyViteX™(PVX) Bloodagar Genital,Suppuration,Throat,

Bloodculture,Cerebrospinal

Burkholderia cepacia 33631 BCSA agar Chromogenicmediafordetectionofpathogensincysticfibrosispatients

Pulmonaryspecimens,Sputum,Broncho-alveolarlavages

Campylobacter 43361 Campyloselagar Specificmedia Stools,Fecalcollection

Candida,Fungi,Yeasts 43631/43639 chromIDCandida Chromogenicmediafordirectidentification Throat,Sputum,Bloodculture,Cerebrospinal

Candida,Fungi,Yeasts 43464 chromIDCandida/SGC2 Chromogenicmediafordirectidentification Throat,Sputum,Bloodculture,Cerebrospinal

Clostridium diffi cile 43871 chromIDC.difficile ChromogenicmediaforthefightagainstHAI Stools

Clostridium diffi cile 43431 Clostridiumdifficileagar Specificmedia Stools

E.coliO157:H7 42605 chromIDO157:H7 Chromogenicmediafordirectidentification Stools,Fecalcollection

E.coliO157H7 43391 SMAC CT agar Specificmedia Stools,Fecalcollection

E.coli, Enterobacteriaceae, Enterococci, Non-Enterobacteriaceae, Proteus,Yeasts,Staphylococci

43821/43829 chromIDCPS Chromogenicmediafordirectidentification Urine

E.coli, Enterobacteriaceae, Non-Enterobacteriaceae, Proteus, Streptococci,Yeasts,Staphylococci

411617 chromIDCPS/ColumbiaCNA + 5%sheepblood Chromogenicmediafordirectidentification Urine

E.coli, Enterobacteriaceae, Non-Enterobacteriaceae, Proteus 43081 Eosin-methyleneblueagar Simplemedia Urine,Stools

E.coli, Enterobacteriaceae, Non-Enterobacteriaceae, Proteus 43331/43339 CLED agar Simplemedia Urine

E.coli, Enterobacteriaceae, Non-Enterobacteriaceae, Proteus 43072 CLEDAndradeagar Simplemedia Urine

E.coli, Enterobacteriaceae, Non-Enterobacteriaceae, Proteus, Shigella, Enterococci

43141/43149 MacConkeyagar Simplemedia Urine,Stools,Genital,Sputum,Cerebrospinal

E.coli, Enterobacteriaceae, Proteus, Enterococci 43021 BCP agar Simplemedia Urine,Stools,Genital

Enterobacteriaceae, Non-Enterobacteriaceae 43341 Drigalskiagar Simplemedia Urine,Stools

Enterobacteriaceae Resistance 43481 chromIDESBL ChromogenicmediaforthefightagainstHAI Screeningspecimens

Enterobacteriaceae Resistance 43861 chromIDCARBA ChromogenicmediaforthefightagainstHAI Screeningspecimens

LIST OF MICRO-ORGANISMS

47

MICRO-ORGANISMS REF. NAME PLATE CLASSIFICATION SPECIMENS

Enterococci 43151 D-Coccosel agar Specifi c media All

Enterococci Resistance 43004 chromID VRE Chromogenic media for the fi ght against HAI Stools, Rectal swabs

Gardnerella 43411/43039 Gardnerella agar Specifi c media Genital

Haemophilus 43681/43689 Haemophilus Chocolate 2 agar Blood agar Genital, Throat, Sputum

Helicobacter pylori 413193 Pylori agar Specifi c media Gastric biopsies

Legionella 43031 Legionella GVPC agar Specifi c media Sputum

Moulds, Candida, Fungi, Yeasts 43651/43659 Sabouraud Gentamicin Chloramphenicol 2 agar Specifi c media

Genital, Suppuration, Throat, Sputum, Blood culture, Cerebro spinal

Mycoplasma 43003 A7 Mycoplasma agar Specifi c media Genital

Neisseria 43611 Chocolate agar + PolyVitex VCAT3 agar Blood agar Throat

Pseudomonas aeruginosa 43565 Cetrimide agar Specifi c media All

Pseudomonas aeruginosa 43462 chromID P.aeruginosa Chromogenic media for detection of pathogens in cystic fi brosis patients Pulmonary specimens

Salmonella 43621/43629 chromID Salmonella Chromogenic media for direct identifi cation Stools, Fecal collection

Salmonella, Shighella 43465 chromID Salmonella / Hektoën Chromogenic media for direct identifi cation Stools, Fecal collection

Salmonella, Shighella 43091/43099 SS agar Simple media Stools

Salmonella, Shighella 43111/43119 Hektoen agar Simple media Stools

S. aureus Resistance 43451/43459 chromID MRSA Chromogenic media for the fi ght against HAI Nose, Throat, Perineum…

Staphylococci 43371 chromID S.aureus Chromogenic media for detection of pathogens in cystic fi brosis patients

Genital, Suppuration, Throat, Sputum…

Staphylococci 43466 chromID MRSA / chromID S.aureus Chromogenic media for the fi ght against HAI Nose, Throat, Perineum…

Staphylococci 43671/43679 Mannitol Salt 2 agar Simple media Suppuration, Throat, Sputum…

Streptococcus agalactiae 43461 chromID Strepto B Chromogenic media for prevention of perinatal group B streptococcal disease

Genital, Urine, Ingested gastric fl uid

Streptococcus agalactiae 43712 Granada agar Chromogenic media for prevention of perinatal group B streptococcal disease


Streptococcus agalactiae 42722 Granada Biphasic broth Chromogenic media for prevention of perinatal group B streptococcal disease Genital swabs

Streptococcus agalactiae 43467 Granada / Columbia CNA + 5% sheep blood

Chromogenic media for prevention of perinatal group B streptococcal disease


V. cholerae, V. parahaemolyticus 43762 chromID Vibrio Chromogenic media for direct identifi cation Stools

Yersinia 43421/43209 Yersinia CIN agar Specifi c media Stools

Susceptibility testing 43301/43309 Mueller Hinton 2 agar Media for susceptibility testing - Etest

43321/43329 Mueller Hinton 2 agar + 5% sheep blood Media for susceptibility testing - Etest

43901/ 43919/43904

Mueller Hinton agar with 5% horse blood + 20 mg/l ß-NAD Media for susceptibility testing - Etest

411968 Brucella blood agar Media for susceptibility testing - Etest

AEB 122180/182 RPMI agar Media for susceptibility testing - Etest

48

Thisdocumentisnotlegallybinding.bioMérieuxreservestherighttomodifyspecificationswithoutnotice.Productavailabilityissubjecttovalidationandterritorialauthorization.Foravailability,pleaseconsultbioMérieuxoryourlocalrepresentative.

LIST OF CULTURE MEDIA

AND ADDITIONALPRODUCTS

PRODUCTS IN ALPHABETICAL ORDER

PRODUCT NAME CODES REFERENCES

A7 Mycoplasma agar MYCO 43003 PLATES 10x55mm

Anaer indicator (forusewithGENboxanaer&GENbaganaer) 96118 50strips

Bacitracin test BAC-F 55902 2x15discs

Baird Parker medium BP 43521* PLATES 20x90mm 43529* PLATES 100x90mm

BCSA agar BCSA 33631 PLATES 20x90mm

BCP agar BCP 43021 PLATES 20x90mm(PurpleLactoseagar) BCP-F 41354 BOTTLES 6x200ml4 week storage at 15°-25°c BCP-D 51035 DEHYDRATED 500g

Bi-BoxTM Count-Tact® 96301* x10forCount-Tactplates

Bi-Box 90 96311* x10for90mmplatesSterileboxforcollecting,transporting&incubating

Blood agar BASA-D 51039 DEHYDRATED 500g

BLSE agar BLSE AEB 525770 BIPLATES 20x90mm

Brain-Heart infusion broth BHI-T 42081 TUBES 20x9ml BHI-D 51009 DEHYDRATED 500g

Brucella Blood agar BBA 411968 PLATES 20x90mm

Campylosel agar CAM 43361 PLATES 20x90mmAdditional product:Campyloselmixture CAM-M 55712 4x2ml(lyo)

Cefinase CEF-F 55622 50discs

Cefixime - Tellurite mixture CT-M 42606 6x4ml(lyo)

Cetrimide agar CET 43565 PLATES 20x90mm

Chocolate agar + PolyViteXTM PVX 43101 PLATES 20x90mm4 week storage at 15°-25°c 43109 PLATES 100x90mm CHOCOPVX-T 42079 TUBES 20(slanted) CHOCO-F 41536 BOTTLES 6x100mlAdditional product:PolyViteX PVX-M 55651 4x1ml(lyo+solvent) 55652 4x10ml(lyo+solvent)

Chocolate agar + PolyViteX VCAT3 VCA3 43611 PLATES 20x90mm4 week storage at 15°-25°c

Chocolate Haemophilus 2 agar HAEM2 43681 PLATES 20x90mm 43689 PLATES 100x90mm

chromID range

chromID Candida agar CAN2 43631 PLATES 20x90mm 43639 PLATES 100x90mm

chromID Candida / Sabouraud CAN2/SGC2 43464 BIPLATES 20x90mmGentamicin Chloramphenicol 2 agar

chromID C.difficile agar CDIF 43871 PLATES 20x90mm

chromID CPS agar CPS 43821 PLATES 20x90mm1 week storage at 15°-25°c 43829 PLATES 100x90mm

chromID CPS / Columbia CNA + 5% sheep blood CPS/CNA 411617 BIPLATES 20x90mm CPS3/CNA 43473

chromID O157H7 agar O157:H7ID-F 42605 BOTTLES 6x200mlAdditional product:Cefixime-Telluritemixture CT-M 42606 6x4ml(lyo)

chromID P.aeruginosa agar PAE 43462 PLATES 20x90mm

NEW

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49

chromID Salmonella agar SM2 43621 PLATES 20x90mm 43629 PLATES 100x90mm

chromID Salmonella / Hektoen agar SM2/HEKT 43465 BIPLATES 20x90mm

chromID S.aureus agar SAID 43371 PLATES 20x90mm

chromID Strepto B agar STRB 43461 PLATES 20x90mm

chromID VIBRIO agar VIB 43762 PLATES 20x90mm

chromID range / Screening of Multidrug - Resistant Organisms

chromID CARBA agar CARB 43861 PLATES 20x90mm

chromID ESBL agar ESBL 43481 PLATES 20x90mm

chromID ESBL / chromID VRE agar ESBL/VRE 43470 BIPLATES 20x90mm

chromID MRSA agar MRSA 43451/43472 PLATES 20x90mm 43459/43479 PLATES 100x90mm

chromID MRSA agar MRSA 43841 PLATES 20x90mmFDAapproved-USAonly

chromID MRSA /chromID S.aureus agar MRSA/SAID 43466 BIPLATES 20x90mm

chromID VRE agar VRE 43004 PLATES 20x90mm

chromID VRE agar VRE 43851 PLATES 20x90mmFDAapproved-USAonly

CLED agar CLED 43331 PLATES 20x90mm4 week storage at 15°-25°c 43339 PLATES 100x90mm CLED-F 41594 BOTTLES 6x200ml CLED-D 51052 DEHYDRATED 500g

CLED Andrade agar CLEA 43072 PLATES 20x90mm

Clostridium difficile agar CLO 43431 PLATES 20x90mmAdditional product:Clostridiumdifficilemixture CLO-M 55691 4x2ml(lyo)

CNA mixture CNA-M 55673 8x2ml(lyo)

Coletsos medium COLETSOS-T 42082 TUBES 20(slanted)

Color Gram 2 packcontainingR1,R2,R3,R4(240mleach) COLORGRAM2-F 55542

R1 COLORGRAM2R1-F 55545 1x2liters R2 COLORGRAM2R2-F 55546 1x2litersR3 COLORGRAM2R3-F 55547 1x2litersR4 COLORGRAM2R4-F 55548 1x2litersTaps 55541 4

Columbia agar COLUMBIA-F 41244 BOTTLES 6x200ml COLUMBIA-D 51026 DEHYDRATED 500gAdditional product:VCNmixture VCN-M 55663 8x1ml(lyo) 55664 8x10ml(lyo)CNAmixture CNA-M 55673 8x2ml(lyo)Clostridiumdifficilemixture CLO-M 55691 4x2ml(lyo)Campyloselmixture CAM-M 55712 4x2ml(lyo)

Columbia agar + 5% horse blood COH 43050 PLATES 20x90mm 43059 PLATES 100x90mm

Columbia agar + 5% sheep blood COS 43041 PLATES 20x90mm 43049 PLATES 100x90mm

Columbia CNA agar COLANC-F 41286 BOTTLES 6x100ml

Columbia CNA agar + 5% sheep blood CNA 43071 PLATES 20x90mm 43079 PLATES 100x90mm

NEW

NEW

PRODUCT NAME CODES REFERENCE

50




Count-Tact CT 43501* PLATES 20x55mm

Count-Tact chromID ESBL CT ESBL 411673* PLATES 20x55mm

Count-Tact chromID MRSA CT MRSA 410155* PLATES 20x55mm

Count-Tact Applicator 96300*

Count-Tact Withoutneutralizers CTTSA 43582* PLATES 20x55mm

Count-Tact Sabouraud Dextrose SAB 43580* PLATES 20x55mmChloramphenicolNeutralizersagar

Count-Tact 3P Irradiated agar CT3P 43691* PLATES 20x55mmstorage at 2°-25°c 43699* PLATES 100x55mm

Count-Tact Sabouraud Dextrose Agar 3P Withneutralizers SABIRR 43812* PLATES 20x55mmstorage at 2°-25°c

D-Coccosel agar DCO 43151 PLATES 20x90mm DCO-T 42083 TUBES 20(slanted) DCO-D 51025 DEHYDRATED 500g

Dermatophyte agar DERM 43062 PLATES 20x90mm DERMATO-T 42084 TUBES 20(slanted)

Drigalski agar DRIG 43341 PLATES 20x90mm4 week storage at 15°-25°c DRIG-F 41314 BOTTLES 6x200ml

Endo agar END 43231 PLATES 20x90mm 43239 PLATES 100x90mm

Eosin-methylene blue agar EMB 43081 PLATES 20x90mm EMB-D 51033 DEHYDRATED 500g

Esculin agar ESCU-T 42086 TUBES 20agardeep

eSwab: flocked swab + AMIES liquid transport regular 41992 50units+50tubespediatric 41991 50units+50tubesurogenital 41990 50units+50tubes

eSwab: dry flocked swab 280101 NUCLISENSREAGENT 100units

Gardnerella agar GAR 43411 PLATES 20x90mm

GENbag :clipsealsx10 45511 microaer 45532 20testsCO2 45533 20testsanaer 45534 20testsanaerindicator 96118 50strips

GENbox :anaer 96124 10sachetsmicroaer 96125 10sachetsCO2 96126 10sachetsJar2.5liters 96127 1unitJar7.0liters 96128 1unitanaerindicator 96118

Gonoline DUO 2 GONODUO2 56528 10agarslides+ 20CO2generatingtablets

Granada™ agar GRAN 43712 PLATES 20x90mm

Granada agar/Columbia CNA + 5% sheep blood GRAN/CNA 43467 BIPLATES 20x90mm

Granada biphasic broth GRANADA-T 42722 TUBES 40tubes(lyo)

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51

Hektoen agar hEkt 43111 platEs 20x90mm 43119 platEs 100x90mm HEKT-F 41554 BOTTLES 6X200ml HEKT-D 51050 dEhYdratEd 500g

Hémoline anaerobic performance HEMOLINEANA-F 52600 BOTTLES 12X100ml

Hémoline diphasic performance HEMOLINEDIPH-F 52510 BOTTLES 12X40ml

Hémoline Duo performance HEMOLINEDUO-F 52800 BOTTLES 6X2bottles(diphasic+anaerobic)

ID color catalase ID-ASE 55561 2x5ml

ID indole-tDa ID-TDA 56541 R1:2.5mlR2:2.5ml

kanamycin-Vancomycin mixture KV-M 55682 8x1ml(lyo)

kligler agar KLIGLER-T 42087 TUBES 20(slant+butt) KLIGLER-D 51059 dEhYdratEd 500g

kovacs - reagent 55631 1x25ml

legionella agar LEG-F 41054 6X200mlAdditional product:Enrichmentsupplement LEGSUP-M 55641 4x2ml(lyo)Mixtureforselectiveisolation LEGSEL-M 55645 4x2ml(lyo)

legionella gVPc agar GVPC 43031 PLATES 20x90mm 43032 platEs 100x90mmBcye with l-cysteine BcYE 43013* PLATES 10x90mmBcye without l-cysteine BcY 43023* PLATES 10x90mm

loeffler agar LOEFFLER-T 42088 TUBES 20(slanted)

löwenstein-Jensen medium LJ-T 42089 TUBES 20(slanted)

löwenstein-Jensen medium + tcH LJTCH-T 42107 TUBES 20(slanted)

Macconkey agar mck 43141 PLATES 20x90mm4 week storage at 15°-25°c 43149 PLATES 100x90mm

Macconkey / columbia cna + 5% sheep blood MCK/CNA 43474 BIPLATES 20x90mm

Macconkey agar without crystal violet MC-D 51036 DEHYDRATED 500g

Mannitol salt 2 agar MSA2 43671 PLATES 20x90mm4 week storage at 15°-25°c 43679 PLATES 100x90mm MSA-T 42080 TUBES 20(slanted) MSA-F 41606 BOTTLES 6x200ml MSA-D 51053 DEHYDRATED 500g

Mueller Hinton 2 agar MH2 43301 PLATES 20x90mm 43309 PLATES 100x90mm 43302 PLATES 20x145mm 43511 PLATES 20x120x120mm MH2-F 41864 BOTTLES 6x200ml MH2-D 51075 DEHYDRATED 500g

Mueller Hinton 2 agar + 5% sheep blood mhs 43321 PLATES 20x90mm 43329 PLATES 100x90mm 43324 PLATES 20x120x120mm

Mueller Hinton agar +5% horse blood + naD mhf 43901 PLATES 20x90mm 43919 PLATES 100x90mm 43904 PLATES 20x120x120mm

Mycoline mYcoliNE 56525 10slides

Mycoplasma Ist 2 MYCOPLASMAIST2 42505 25tests

neW

product name codes reference

52




Mycoplasma Preparation 42507 8x3.1ml

ONPG Discs ONPG 55601 30discs

Optochin test OPTO-F 55912 2x30discs

Oxidase Reagent OXI-T 55635 50ampules

Peptone water PW-T 42085 TUBES 20x9ml

PolyViteX POLYVITEX-M 55651 4x1ml(lyo) 55652 4x10ml(lyo)

Portagerm PORT-T 42105 TUBES 20(butt) PORT-F 41995 BOTTLES 10(butt)

Portagerm Amies Agar + Swab PORT-AA 41999 50units

Portagerm Amies Agar + Swab - Sterile zone PORT-AASZ 41998 10units

Portagerm pylori PORT-PYL 42041 BOTTLES 8x2.5ml

PREVI™ Fluo TB 411009 R1-R4:250mlR2-R3-R5-R6:125ml

Pylori agar PYL 413193 PLATES 20x90mm

Rabbit plasma Detection of staphylocoagulase STAPH-ASE 55181 8x0.5ml(lyo) 55182 1x3.5ml(lyo)

Rappaport broth RAPPAPORT-T 42091 TUBES 20x9ml

RPMI agar RPMI AEB 122180 PLATES 10x90mm AEB 122182 PLATES 10x140mm

Sabouraud 2 agar SAB2-T 42037 TUBES 20(slanted) SAB2-F 42066 BOTTLES 6x100ml SAB2-D 51020 DEHYDRATED 500g

Sabouraud 2 Chloramphenicol agar SAB2CHL-T 42038 TUBES 20(slanted) SAB2CHL-F 42067 BOTTLES 6x100ml SAB2CHL-D 51021 DEHYDRATED 500g

Sabouraud Chloramphenicol Actidione agar SABCHLACTI-T 42094 TUBES 20(slanted)

Sabouraud Dextose agar SDA 43555 PLATES 20x90mm

Sabouraud Gentamicin Chloramphenicol 2 agar SGC2 43651 PLATES 20x90mm4 week storage at 15°-25°c 43659 PLATES 100x90mm SAB2GENTACHL-T 42031 TUBES 20(slanted) SAB2GENTACHL-F 42056 BOTTLES 6x100ml

Sabouraud liquid broth SABB-T 42108 TUBES 20x9ml

Sabouraud Tetrazolium Gentamicin Chloramphenicol agar SABTTZ-T 42096 TUBES 20(slanted)

Saline solution NaCl0.85%-T 42113* TUBES 100x9ml

Schaedler agar + 5% sheep blood SCS 43401 PLATES 20x90mm 43279 PLATES 100x90mm

Schaedler broth + 0.02% agar + Vit K3 SCHAEDK30.02%-T 42097 TUBES 20x13mlSchaedler broth + 0.2% agar + Vit K3 SCHAEDK30.2%-T 42098 TUBES 20x13mlSchaedler broth + Vit K3 SCHAEDK3-T 42106 TUBES 20x13ml

Schaedler Neo. Vanco. + 5% sheep blood SNVS 413194 PLATES 20x90mm

Selenite F broth SELENITEF-T 42099 TUBES 20x9ml

SMAC CT agar SMAC 43391 PLATES 20x90mmCefixime - Tellurite mixture CT-M 42606 6x4ml(lyo)

SS agar SS 43091 PLATES 20x90mm4 week storage at 15°-25°c 43099 PLATES 100x90mm SS-D 51043 DEHYDRATED 500g

*Fo

rmicr

obio

logi

calc

ontro

lonl

y


NEW

NEW

53

staining of acid-fast organisms Kinyounsolution BKK-F 55521 1x450mlGabettsolution BKG-F 55531 1x450ml

thioglycolate broth with resazurin THIO-T 42074 TUBES 20x9ml

todd Hewitt broth + antibiotics TODD-T 42116 TUBES 20x9ml

treatment of expectoration specimens for the investigation of mycobacteria Reagent1-L-cysteine 55211 5gReagent2-benzalkoniumchloride 42104 20x10ml 55222 1x450mlReagent3-phosphatebufferpH6.8 42103 20x15ml 55232 1x450mlReagent4-0.2%bovinealbumin 55242 16x10ml

trypcase soy agar tsa 43011 PLATES 20x90mm 43019 PLATES 100x90mm TSA-T 42101 TUBES 20(slanted) TSA-F 41466 BOTTLES 6x200ml 41467 BOTTLES 6x100ml

trypcase soy agar + 5% horse blood tsh 43061 PLATES 20x90mm

trypcase soy agar + 5% sheep blood tss 43001 PLATES 20x90mm 43009 PLATES 100x90mm

trypcase soy broth TSB-T 42100 TUBES 20x9ml

trypcase soy 3P Irradiated agar TSA3 43711* PLATES 20x90mmstorage at 2°-25°c30gofculturemedium 43169* PLATES 100x90mm

tsI agar TSI-D 51047 DEHYDRATED 500g

urea-arginine lyo 2 broth 42508 25tests

urea-indole medium UI-F 55752 BOTTLES 10x10ml

uriline URILINE 56507 10dipslides 56508 100dipslides

uriline 3 coli URILINE3 56527 10dipslides

uriline 3 enterococcus URILINE3 56526 10dipslides ENTEROCOCCUS

XlD agar xld 43563 PLATES 20x90mm 43564 PLATES 100x90mm XLD-D 51049 DEHYDRATED 500g

yersinia cIn agar YEr 43421 PLATES 20x90mm 43209 PLATES 100x90mm

product name codes reference

markedproducts

54

NOteS

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55

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. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

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. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

56

NOteS

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

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. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

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. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

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. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

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. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

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. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

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. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

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bioMérieux S.A.69280 Marcy l’EtoileFranceTel. : 33 (0)4 78 87 20 00Fax : 33 (0)4 78 87 20 90

www.biomerieux.comwww.biomerieux-diagnostics.com

ATCC

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Documents

culture - bioMérieux España...chromID CPS® agar, an isolation medium for urine specimens, is fully compatible with 23 identiﬁcation reagents and with 10 AST reagents including