--CURRICULUM VITAEbtc.wustl.edu/iscgt14/CVHansClevers.pdf · Curriculum vitae J.C. Clevers (1-5-2014) 14) Keynote Lecture at the Dutch-German meeting of Molecular Cardiology groups

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Curriculum vitae J.C. Clevers (1-5-2014)

--CURRICULUM VITAE

Name Johannes Carolus Clevers Address Singel 34, 1015 AA Amsterdam, The Netherlands Date of Birth March 27, 1957 Place of Birth Eindhoven, The Netherlands Marital Status Married, two children

Education

1982 M.Sc. ("Doctoraal") in Biology. University of Utrecht 1984 M.D. ("Artsexamen") University of Utrecht 1985 Ph.D. ("Promotie") University of Utrecht

Scientific Training/positions

1985 – 1989 Research Fellow in Pathology. Dana-Farber Cancer Institute, Harvard Medical School, Boston MA, USA

1989 – 1991 Universitair Docent, Department of Clinical Immunology, University of Utrecht

1991 – 2002 Professor and Chairman, Dept. of Immunology, Faculty of Medicine, University of Utrecht

2002 – 2012 Director of Hubrecht Institute, Developmental Biology and Stem Cell Research

2002 – Professor in Molecular Genetics. The Academic Biomedical Centre, University of Utrecht

2002 – Honorary professor at Changsha-Hunan, China

2012-2016 President of the Royal Netherlands Academy of Sciences

Membership etc

1999 - Member European Molecular Biology Organisation (EMBO)

2000 - Member of the Royal Netherlands Academy of Sciences

2004 - Member of the International Society for Stem Cell Research (ISSCR)

2005 - Member of the Scientific Advisory Board of ISREC, Swiss Institute for Experimental Cancer Research

2006 - Member Scientific Committee of the Louis-Jeantet Foundation for Medicine

2006 - Member of the American Association for Cancer Research (AACR)

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2006-2008 President of the International Society of Differentiation (ISD)

2007 - Member of the National Scientific Advisory Board NKI-AVL

2008 - Member of the scientific advisory board of “de Anatomische Les”

2009 - Member of the Academia Europaea

2010 - Member of the Scientific Advisory Board of the MRC Clinical Sciences Centre

2012- Member of the American Academy of Arts and Sciences

2012- Member of the ‘Koninklijke Hollandsche Maatschappij der Wetenschappen’ (The Royal Holland Society of Sciences and Humanities)

2012- Member of the Gairdner Foundation medical advisory board

Editorial boards

2004 - Member of the Editorial Board of the EMBO Journal

2007 - Member of the of the Editorial Board of Gastroenterology

2008 - Member of the Editorial Board of the Journal: Diseases, Models and Mechanisms

2009 - Member of the Editorial Board of Cell

2010 - Member of the Editorial Board of Genes & Development

2011 - Member of the Editorial Board of Gastroenterology Report

2012- Member of the Editorial Board of Stem Cell Reports

2013- Member of the Editorial Board of Cell Stem Cell

Prizes, awards

2000 Catharijne-prize for medical science

2001 Award from the European Society for Clinical Investigation

2001 Spinoza-award

2004 Louis-Jeantet Prize for Medicine

2005 the Science and Society Prize

2005 the French honor of “Chevalier de la Legion d'Honneur”

2005 Katharine Berkan Judd Award

2006 Rabbi Shai Shacknai Memorial Prize for Immunology and Cancer Research

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2008 Josephine Nefkens Prize for Cancer Research (Erasmus MC, Rotterdam)

2008 Meyenburg Cancer Research Award

2009 The Queen Wilhelmina Dutch Cancer Society Award

2010 The United European Gastroenterology Federation (UEGF) Research Prize

2011 The Ernst Jung Medical Award

2011 Kolff prize

2012 Association pour la Recherche sur le Cancer (ARC) Léopold Griffuel Prize

2012 William Beaumont prize of the American Gastroenterology Association

2012 The Heineken Prize for Medicine

2012 Ridder in de Orde van de Nederlandse Leeuw

2013 The Breakthrough Prize in Life Sciences

2014 Massachusetts General Hospital Award in Cancer Research

2014 TEFAF Oncology Chair 2014

2014 Fellow of the AACR Academy

Keynotes and Named Lectures Hans Clevers:

1) David Kipnis Lecture, Washington University, St. Louis, MO (May 22, 2003) 2) Presentation in accordance with the Louis-Jeantet Prize for Medicine, Geneva (April 22,2004) 3) Karolinska Resarch Lecture at Nobel Forum, Huddinge, Stockholm (September 14, 2004) 4) Shapiro Lecture, University of Pennsylvania, Philadelphia (March 31, 2005) 5) Judd Award Lecture, Memorial Sloan Kettering Cancer Center, New York (May 11, 2005) 6) 18th Adam Neville Lecture, University of Dundee (June 3, 2005) 7) The Rabbi Shai Shacknai Lectureship award, The Hebrew University (February 5-7, 2006) 8) Nishizuka Lecture at 20th IUBMB International Congress of Biochemistry and Molecular Biology

and 11th FAOBMB Congress, Kyoto (June 22, 2006) 9) Keynote Lecture at the 2007 Keystone Symposium on Molecular Targets for Cancer, Whistler,

BC, Canada (Marc 18, 2007) 10) Keynote Lecture at the Gordon Conference on Cell Contact and Adhesion, Il Ciocco, Barga, Italy

(May 27, 2007) 11) Ebling Lecturer: Organ Development at the 5th International Congress of Hair Research,

Vancouver (June 15, 2007) 12) Keynote Lecture at San Diego Wnt Conference, University of California, San Diego (June 21,

2007) 13) Keynote Lecture at the New Frontiers in Life Sciences Symposium, Radboud University,

Nijmegen (October 26, 2007)

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14) Keynote Lecture at the Dutch-German meeting of Molecular Cardiology groups 2008, Amsterdam (February 7, 2008)

15) Keynote Lecture at the European Multidisciplinary Colorectal Cancer Congress 2008, Berlin (February 24, 2008)

16) Keynote Lecture at the 2008 Keystone Symposium on Signaling Pathways in Cancer and Development, Steamboat Springs, Colorado (March 24, 2008)

17) Keynote lecture at the Giovanni Armenise-Harvard Foundation 12th annual symposium Stresa, Italy (June 20, 2008)

18) PCDI (Postdoc Career Development Initiative) Retreat, Heeze, the Netherlands (November 7, 2008)

19) Keynote lecture at the FASEB Summer Research Conference entitled: Gastrointestinal Tract XIII: Advances in the Molecular & Cell Biology of the Intestinal Epithelium: Development, Inflammation, Host Defense & Cancer, Snowmass Village, CO (August 9, 2009)

20) Keynote lecture at the EMBO Molecular Medicine Workshop “Invasive Growth: a Genetic Programme for Stem Cells and Cancer” in Turin (September 12, 2009.)

21) Keynote lecture at Biannual Meeting on Stem Cells, Cold Spring Harbor Laboratory (September 25, 2009)

22) Keynote lecture at BioValley Life Sciences Week / MipTec Conference (October 13, 2009) 23) Leukaemia Research Annual Guest Lecture, London (November 11, 2009) 24) Keynote lecture at James Watson Cancer Symposium, Suzhou, China (April 8, 2010) 25) David de Wied-lezing, University Utrecht (May 28, 2010) 26) Keynote lecture International Student Congress of Medical Sciences (ISCOMS), University

Medical Center Groningen (June 9, 2010) 27) Keynote lecture at the 5th lustrum of the training of the Biomedical Sciences of the Utrecht

University (June 11, 2010) 28) Keynote lecture at the Falk symposium: IBD Benelux Summit 2010, Maastricht (June 25, 2010) 29) Sir Richard Gardner Celebratory Lecture, University of Scotland (November 5, 2010) 30) Keynote lecture at Fortaleza Conference (Brasil), December 7, 2010 31) Keynote lecture at Tornado WP3-WP4 meeting: Exploring the interaction between man and

microbiata, Lausanne, January 24, 2011 32) Max Birnstiel lecture at the Research Institute of MolecularPathology, Vienna (February 2, 2011) 33) Keynote lecture at the 7th annual Swiss Stem Cell Network meeting, Lausanne (February 4,

2011) 34) Streisinger Lecture at the University of Oregon (February 22, 2011) 35) Mendel Lecture, Brno (May 12, 2011) 36) Keynote lecture at the 97th Annual Meeting of the Japanese Society of Gastroenterology, Tokyo

(May 15, 2011) 37) Keynote lecture at the COE symposium at the Keio University, Tokyo (May 16, 2011) 38) Keynote lecture at the BMM/TeRM Annual Meeting 2011, Ermelo (May 25, 2011) 39) Keynote Lecture at the FASEB Summer Research Conference: Gastrointestinal Tract XIV: Stem

Cells, Adaptation, Inflammation and Cancer (August 14, 2011) 40) The Weigle lecture at the University of Geneva (October 18, 2011) 41) Keynote lecture at the joined meeting of the Dutch endothelial Biology Society and the Dutch Cell

Biology Society, KNAW Amsterdam (October 21, 2011) 42) Plenary talk at EuroSystem-Advances in Stem Cell Research VI Conference, Kranjska Gora,

Slovenia (January 23, 2012) 43) Keynote lecture at the 16th Molecular Medicine Day, Rotterdam (February 29, 2012)

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44) Keynote Lecture at the Keystone Symposium: The Life of a Stem Cell: From Birth to Death, Olympic Valley, CA (March 11, 2012)

45) Provost's Lecture, MD Anderson Cancer Center (March 16, 2012) 46) Keynote Lecture at the 24th Annual Meeting of the European Renal Cell Study Group, Doorwerth,

the Netherlands (March 22, 2012) 47) Keynote Lecture at the Abcam Stem Cell Symposium, Boston (April 30, 2012) 48) Keynote Lecture at the 3rd EMBO Conference Series on Cellular Signaling & Molecular Medicine,

Dubrovnik (May 28, 2012) 49) Mühlbock Memorial Lecture at the 22nd Biennial Congress of the European Association for

Cancer Research (EACR), Barcelona (July 7, 2012) 50) Keynote Lecture at the Cell Tumour Microenvironment Lablinks symposium, London (July 9,

2012) 51) Keynote Lecture at the Heinrich F. C. Behr-Symposium, Heidleberg (October 16, 2012) 52) Keynote Lecture at the Food Valley Expo, Arnhem (October 25, 2012) 53) Jean Brachet Memorial Lecture at the International Society of Differentiation Conference "Stem

Cells, Development and Regulation, Amsterdam (November 6, 2012) 54) Keynote lecture at the Student Research Conference 2012, Utrecht (November 21, 2012) 55) van Leeuwenhoek Lecture on BioScience, Leiden (February 28, 2013) 56) Keynote lecture at the 8th Cancer Scientific Forum of the Cancéropôle CLARA, Lyon (March 21,

2013) 57) The opening plenary lecture at the AACR Annual meeting, Washington (April 7, 2013) 58) CeMM Landsteiner Lecture, Vienna (May 6, 2013) 59) Methusalem Lecture, Leuven (May 24, 2013) 60) EMBO Keynote Lecture at FASEB SRC "The TGF-B Superfamily: Signaling in Development &

Diseases", Steamboat Springs, Colorado (July 28, 2013) 61) Keynote lecture at the AACR Third International Conference on Frontiers in Basic Cancer

Research 2013, Washington (September 18, 2013) 62) State of the Art Lecture at the anniversary conference of the Nederlandse Vereniging voor

Gastroenterologie, Veldhoven (October 3, 2013) 63) Keynote at the 2013 European Cancer Congress, Amsterdam (September 28, 2013) 64) Keynote Lecture at the AACR-NCI-EORTC International Conference on Molecular Targets and

Cancer Therapeutics, Boston (October 20, 2013) 65) Plenary lecture at the 2014 FISEB (Federation of the Israeli Societies for Experimental Biology)

Conference, Eilat (February 12, 2014)

Key Publications J.C. Clevers:

1) van de Wetering, M., Oosterwegel, M., Dooijes, D., and Clevers, H.C. Identification and cloning of TCF-1, a T cell-specific transcription factor containing a sequence-specific HMG box. EMBO J. 10:123-132 (1991)

2) Verbeek, J.S., Ison, D., Hofhuis, F., Robanus-Maandag, E., te Riele, H., van de Wetering, M., Oosterwegel, M., Wilson, A., MacDonald, H.R. and Clevers, H.C. An HMG box containing T-cell factor required for thymocyte differentiation. Nature 374: 70-74 (1995)

3) Schilham, M., Oosterwegel, M., Moerer, P., Jing Ya, de Boer, P., van de Wetering, M., Verbeek, S., S., Lamers, W., Kruisbeek, A., Cumano, A., and Clevers, H.Sox-4 gene is required for cardiac outflow tract formation and pro-B lymphocyte expansion. Nature 380: 711-714 (1996)

4) Molenaar, M., Van de Wetering, M., Oosterwegel, M., Peterson-Maduro, J., Godsave, S., Korinek, V., Roose, J., Destrée, O. And Clevers, H. Xtcf-3 Transcription factor mediates beta-

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catenin-induced axis formation in xenopus embryos. Cell 86: 391-399 (1996)

5) Korinek, V, Barker, N., Morin, P.J., van Wichen, D., de Weger, R., Kinzler, K.W., Vogelstein, B., and Clevers, H. Constitutive Transcriptional Activation by a beta-catenin-Tcf complex in APC-/- Colon Carcinoma. Science 275: 1784-1787 (1997)

6) Morin, P.J., Sparks, A., Korinek, V., Barker, N., Clevers, H., Vogelstein, B., and Kinzler, K. Activation of beta-catenin-Tcf signaling in colon cancer by mutations in beta-catenin or APC. Science 275: 1787-1790 (1997)

7) van de Wetering, M., Cavallo, R., Dooijes, D., van Beest, M., van Es, J., Loureiro, J., Ypma, A., Hursh, D., Jones, T., Bejsovec, A., Peifer, M., Mortin, M., and Clevers, H. Armadillo co-activates transcription driven by the product of the Drosophila segment polarity gene dTCF. Cell 88, 789-799 (1997)

8) Korinek, V., Barker, N., Moerer, P., van Donselaar, E., Huls, G., Peters, P.J. and Clevers, H. Depletion of epithelial stem cell compartments in the small intestine of mice lacking Tcf 4. Nat Genet 19: 379 383 (1998)

9) Roose, J., Molenaar, M., Peterson, J., Hurenkamp, J., Brantjes, H., Moerer, P., van de Wetering, M., Destree, O., and Clevers, H. The Xenopus Wnt effector XTcf-3 interacts with Groucho-related transcriptional repressors. Nature 395: 608-612 (1998)

10) Roose, J., Huls, G., van Beest, M., Moerer, P., van der Horn, K., Goldschmeding, R., Logtenberg, T., and Clevers, H. Synergie between tumor suppressor APC and the beta-catenin/Tcf4 target gene Tcf1. Science 285: 1923-1926 (1999)

11) Korswagen, R., Herman, M. and Clevers, H. Separate beta-catenins mediate Wnt signaling and cadherin adhesion in C. elegans. Nature 406: 527-532 (2000)

12) Bienz, M., and Clevers, H. Linking colorectal cancer to Wnt signaling. Review Cell 103: 311-320 (2000)

13) van de Wetering, M., Sancho, E., Verweij, C., de Lau, W., Oving, I., Hurlstone, A., van der Horn, K., Batlle, E., Coudreuse, D., Haramis, A-P., Tjon-Pon-Fong, M., Moerer, P., van den Born, M., Soete, G., Pals, S., Eilers, M., Medema, R., Clevers, H. The beta catenin/TCF4 complex imposes a crypt progenitor phenotype on colorectal cancer cells. Cell 111: 241-250 (2002)

14) Batlle, E., Henderson, J.T., Beghtel, H., van den Born, M., Sancho, E., Huls, G., Meeldijk, J., Robertson, J., van de Wetering, M., Pawson, T., Clevers, H. Beta- catenin and TCF mediate cell positioning in the intestinal epithelium by controlling the expression of EphB/ephrinB. Cell 111: 251-263 (2002)

15) Hurlstone, A.F., Haramis, A.P., Wienholds, E., Begthel, H., Korving, J., van Eeden, F., Cuppen, E., Zivkovic, D., Plasterk, R.H., Clevers, H. The Wnt/beta-catenin pathway regulates cardiac valve formation. Nature 425: 633-637 (2003)

16) Baas, A.F., Kuipers, J., van der Wel, N.N., Batlle, E., Koerten, H.K., Peters, P.J., Clevers H.C. Complete polarization of single intestinal epithelial cells upon activation of LKB1 by STRAD. Cell 116: 457-466 (2004)

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17) Haramis, A.P., Begthel, H., van den Born, M., van Es, J., Jonkheer, S., Offerhaus, G.J., Clevers, H. De novo crypt formation and Juvenile Polyposis upon BMP inhibition. Science 303: 1684-1686 (2004)

18) Radtke, F and Clevers, H., Self-renewal and cancer of the gut: Two sides of a coin. Review Science 307: 1904-1909 (2005)

19) Reya, T., Clevers, H., Wnt signalling in stem cells and cancer. Review. Nature 434: 843-850 (2005)

20) Van Es, J.H., Van Gijn, M.E., Riccio, O., van den Born, M., Vooijs, M., Begthel, H., Cozijnsen, M., Robine, S., Winton, D.J., Radtke, F., Clevers H. Notch pathway/γ-secretase inhibition turns proliferative cells in intestinal crypts and neoplasia into Goblet cells. Nature 435: 959-963 (2005)

21) Batlle, E., Bacani, J., Begthel, H., Jonkheer, S., Gregorieff, A., van de Born, M., Malats, N., Sancho, E., Boon, E., Pawson, T., Gallinger, S., Pals, S., Clevers, H. EphB activity suppresses colorectal cancer progression. Nature 435: 1126-1130 (2005)

22) Clevers, H. Wnt/β-catenin signaling in development and disease, Review Cell 127: 469-480 (2006)

23) Barker, N., Van Es, J.H., Kuipers, J., Kujala, P., Van den Born, M., Cozijnsen, M., Haegebarth, A., Korving, J., Begthel, H., Peters, P.J., Clevers, H. Identification of stem cells in small intestine and colon by the marker gene LGR5. Nature 449: 1003-1007 (2007)

24) Jaks, V., Barker, N, Kasper, M., van Es, J.H., Snippert, H.J., Clevers, H., Toftgård, R. Lgr5 marks cycling, yet long-lived, hair follicle stem cells. Nat Genet. 40: 1291-1299 (2008)

25) Barker, N., Ridgway, R.A., van Es, J.H.,van de Wetering, M., Begthel, H., van den Born, M., Danenberg, E., Clarke, A.R., Sansom, O.J., Clevers, H. Crypt Stem Cells as the Cells-of-Origin of Intestinal Cancer. Nature 457: 608-611 (2009)

26) van der Flier, L.G., van Gijn, M.E., Hatzis, P., Kujala, P., Haegebarth, A., Stange, D.E., Begthel, H., van den Born, M., Guryev, V., Oving, I., van Es, J.H., Barker, N., Peters, P.J., van de Wetering, M. and Clevers, H. Transcription Factor Achaete Scute-Like 2 Controls Intestinal Stem Cell Fate. Cell 136: 903-912 (2009)

27) Sato, T., Vries, R., Snippert, H., van de Wetering, M., Barker, N., Stange, D., van Es, J., Abo, A., Kujala, P., Peters, P., and Clevers, H. Single lgr5 gut stem cells build crypt-villus structures in vitro without a stromal niche. Nature 459 :262-265 (2009)

28) Barker, N., Huch, M., Kujala, P., van de Wetering, M., Snippert, H.J., van Es, J.H., Sato, T., Stange, D.E., Begthel, H., van den Born, M., Danenberg, E., van den Brink, S., Korving, J., Abo, A., Peters, P.J., Wright, N., Poulsom, R., Clevers, H. Lgr5(+ve) stem cells drive self-renewal in the stomach and build long-lived gastric units in vitro. Cell Stem Cell 6: 25-36 (2010)

29) Snippert, H.J., Haegebarth, A., Kasper, M., Jaks, V., van Es, J.H., Barker, N., van de Wetering, M., van den Born, M., Begthel, H., Vries, R.G., Stange, D.E., Toftgård, R., Clevers H. Lgr6 marks stem cells in the hair follicle that generate all cell lineages of the skin. Science 327: 1385-1389 (2010)

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30) Snippert, J., van der Flier, L.G., Sato, T., van Es, J.H., van den Born, M., Kroon-Veenboer, C., Barker, N.,Klein, A.M., van Rheenen, J. Benjamin D. Simons, B.D. and Clevers, H. Intestinal Crypt Homeostasis results from Neutral Competition between Symmetrically Dividing Lgr5 Stem Cells. Cell 143:134-44 (2010)

31) Sato, T., van Es, J.H., Snippert, H.J., Stange, D.E., Vries, R.G., van den Born, M., Barker, N., Shroyer, N.F., van de Wetering, M., Clevers, H. Paneth cells constitute the niche for Lgr5 stem cells in intestinal crypts. Nature 469: 415-418 (2011)

32) de Lau, W., Barker, N., Low, T.Y., Koo, B.K., Li, V.S., Teunissen, H., Kujala, P., Haegebarth, A., Peters, P.J., van de Wetering, M., Stange, D.E., van Es, J., Guardavaccaro, D., Schasfoort, R.B., Mohri, Y., Nishimori, K., Mohammed,S., Heck, A.J., Clevers, H. Lgr5 homologues associate with Wnt receptors and mediate R-spondin signalling. Nature 476: 293-297 (2011)

33) Li, V.S., Ng, S.S., Boersema, P.J., Low, T.Y., Karthaus, W.R., Gerlach, J.P., Mohammed, S., Heck, A.J., Maurice, M.M., Mahmoudi, T. and Clevers H. Wnt signaling inhibits proteasomal β-catenin degradation within a compositionally intact Axin1 complex. Cell 149: 1245-1256 (2012)

34) Koo, B-K., Spit, M. Jordens, I., Low, T.Y., Stange, D.E., van de Wetering, M., van Es, J.H., Mohammed, S., Heck, A.J.R., Maurice, M.M. and Hans Clevers. Tumour suppressor RNF43 is a stem cell E3 ligase that induces endocytosis of Wnt receptors. Nature 488: 665-669 (2012)

35) Schepers, A.G., Snippert, H.J., Stange, D.E., van den Born, M., van Es, J.H., van de Wetering, M., Clevers, H. Lineage Tracing Reveals Lgr5+ Stem Cell Activity in Mouse Intestinal Adenomas. Science 337: 730-735 (2012)

36) van Es, J.H., Sato, T., van de Wetering, M., Lyubimova, A., Yee Nee, A.N., Gregorieff, A., Sasaki, N., Zeinstra, L., van den Born, M., Korving, J., Martens, A.C., Barker, N., van Oudenaarden, A., Clevers, H. Dll1(+) secretory progenitor cells revert to stem cells upon crypt damage. Nat Cell Biol. 14: 1099-1104 (2012)

37) Boj, S,F., van Es, J.H.,Huch. M., Li, V.S., Jose, A., Hatzis, P., Mokry, M., Haegebarth, A., van den Born, M., Chambon, P., Voshol, P., Dor, Y., Cuppenm E., Fillat, C., Clevers, H. Diabetes risk gene and Wnt effector Tcf7l2/TCF4 controls hepatic response to perinatal and adult metabolic demand. Cell 151: 1595-1607 (2012)

38) Huch, M., Dorrell, C., Boj, S.F., van Es, J.H., van de Wetering, M., Li, V.S.W., Hamer, K., Sasaki, N., Finegold, M.J., Haft, A., Grompe, M., Clevers, H. In vitro expansion of single Lgr5+ liver stem cells induced by Wnt-driven regeneration. Nature 494: 247-250 (2013)

39) Sato, T., Clevers, H.. Growing self-organizing mini-guts from a single intestinal stem cell: mechanism and applications. Review Science 340: 1190-1194 (2013)

40) Clevers, H. The intestinal crypt, a prototype stem cell compartment. Cell. 154: 274-284 (2013)

41) Stange, D.E., Koo, B.K., Huch, M., Sibbel, G., Basak, O., Lyubimova, A., Kujalla, P., Bartfeld, S., Koster, J., Geahlen, J.H., Peters, P.J., van Es, J., van de Wetering, M., Mills, J.C., Clevers, H. Differentiated Troy+ chief cells act as ‘reserve’ stem cells to generate all lineages of the stomach epithelium. Cell 155: 357-368 (2013)

Peer-reviewed publications J.C. Clevers

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1) Clevers, H.C., Bloem, A.C., Gmelig-Meyling, F., and Ballieux, R.E. Ligands of surface Ig raise cytoplasmic free Ca++ in human B cells. Scand.J.Immunol. 22: 557-562 (1985)

2) Clevers, H.C., Hoeksema, M., Gmelig-Meyling, F.H.J., and Ballieux, R.E. Calcium ionophore A23187 induces Il2 reactivity in human T cells. Scand.J.Immunol. 22: 633-638 (1985)

3) Clevers, H.C., Versteegen, J.M.T., Logtenberg, T., Gmelig-Meyling, F.H.J., and Ballieux, R.E. Synergistic action of A23187 and phorbol ester on human B cell activation. J.Immunol. 135: 3827-3830 (1985)

4) Clevers, H.C., de Bresser, A., Kleinveld, H., Gmelig-Meyling, F.H.J., and Ballieux, R.E. WGA activates human T lymphocytes by stimulation of phosphoinositide hydrolysis. J.Immunol. 136: 3180-3183 (1986)

5) Bloem, A.C., Clevers, H.C., Bast, E.J.E.G., and Ballieux, R.E. T cells in B cell chronic lymphocytic leukaemia. Clin.Exp.Immunol. 63: 188-193 (1986)

6) Clevers, H.C., Versteegen, J.M.T., Gmelig-Meyling, F.H.J., and Ballieux, R.E. Synergistic action of A23187 and PMA on B cells in CLL. In:Primary Immunodeficiency Diseases. Editors M.M. Eibl and F.S. Rosen, 109-112, Elsevier The Netherlands (1986)

7) Gold, D.P., Clevers, H.C., Alarcon, B., Dunlap, S., Novotny, J., Williams, A.F., and Terhorst, C. Evolutionary relationship between the T3 chains of the T cell receptor complex and the immunoglobulin supergene family. Proc.Natl.Acad.Sci.USA 84: 7649-7653 (1987)

8) Saito, H., Koyama, T., Georgopoulos, K., Clevers, H.C., Haser, W.G., LeBien, T., Tonegawa, S., and Terhorst, C. Close linkage of the mouse and human CD3-g and d genes suggests a control of their transcription by common regulatory elements. Proc.Natl.Acad.Sci.USA 84: 9131-9134 (1987)

9) Hall, C., Alarcon, B., Berkhout, B., Clevers, H.C., Georgopoulos, K., Gold, D., Pettey, C., van den Elsen, P., Wileman, T., and Terhorst, C. CD3: Structural and genetic aspects of a cell surface glycoprotein complex. In: Leukocyte typing III. Editors McMichael et al. 889-895, Oxford University Press, Oxford U.K. (1987)

10) Clevers, H.C., Alarcon, B., Wileman, T., and Terhorst, C. The T cell Receptor/CD3 complex: A dynamic protein ensemble. Annu.Rev.Immunol. 6: 629-662 (1988)

11) Clevers, H.C., Dunlap, S., Saito, H., Georgopoulos, K., Wileman, T., and Terhorst, C. Characterization and expression of the murine CD3-epsilon gene. Proc.Natl.Acad.Sci.USA 85: 8623-8627 (1988)

12) Clevers, H.C., Dunlap, S., and Terhorst, C. The transmembrane orientation of the epsilon chain of the TCR/CD3 complex. Eur.J.Immunol. 18: 705-710 (1988)

13) Clevers, H.C., Dunlap, S., Wileman, T., and Terhorst, C. Human CD3-epsilon gene contains 3 miniexons and is transcribed from a non-TATA promoter. Proc.Natl.Acad.Sci.USA 85: 8156-8160 (1988)

14) Peters, P., Geuze, H.J., van der Donk, H.A., Slot, J.W., Griffith, J.M., Stam N.J., Clevers, H.C., and Borst, J. Molecules relevant for T cell-target cell interaction are present in cytolytic granules of human T lymphocytes. Eur.J.Immunol. 19: 1469-1475 (1989)

15) Clevers, H.C., Lonberg, N., Dunlap, S., Lacy, E., and Terhorst, C. An enhancer located in a CpG-island 3' of the TCR/CD3-e gene confers T lymphocyte-specificity to its promoter. EMBO J. 8: 2527-2535 (1989)

16) Clevers, H.C., MacHugh, N.D., Bensaid, A., Dunlap, S., Baldwin, C.L., Kaushal, A., Iams, K., Howard, C.J., and Morrisson, W.I. Identification of a bovine surface antigen uniquely expressed

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on CD4/CD8 negative TCR-gamma/delta positive T lymphocytes. Eur.J.Immunol. 20: 809-817 (1990)

17) Dobbelaere, D.A., Prospero, T.D., Roditi, I.J., Kelke, C., Baumann, I., Eichhorn, M., Williams, R.O., Ahmed, J.S., Baldwin, C.L., Clevers, H.C. et al. Expression of Tac antigen component of bovine interleukin-2 receptor in different leukocyte populations infected with Theileria parva or Theileria annulata. Infect-Immun. 58: 3847-3855 (1990)

18) Clevers, H.C., and Owen, M. Towards a molecular understanding of T cell differentiation. Immunology Today 12: 86-92 (1991)

19) van de Wetering, M., Oosterwegel, M., Dooijes, D., and Clevers, H.C. Identification and cloning of TCF-1, a T cell-specific transcription factor containing a sequence-specific HMG box. EMBO J. 10: 123-132 (1991)

20) Oosterwegel, M., van de Wetering, M., Dooijes, D., Klomp, L., Winoto, A., Georgopoulos, K., Meijlink, F., and Clevers, H.C. Cloning of the murine TCF-1, a T cell-specific transcription factor interacting with functional motifs in the CD3-epsilon and TCR-alpha enhancers. J.Exp.Med. 173: 1133-1142 (1991)

21) Metzelaar, M., Wijngaard, P., Peters, P., Nieuwenhuis, K., Sixma, J., and Clevers, H.C. CD63 antigen. A novel lysosomal membrane glycoprotein, cloned by a screening procedure for intracellular antigens in eukaryotic cells. J.Biol.Chem. 266: 3239-3245 (1991)

22) Bensaid, A., Kaushal, A., Baldwin, C.L., Clevers, H.C., Young, J.R., Kemp, S.J., MacHugh, N.D., Toye, P.G., and Teale, A.J. Identification of expressed bovine class I MHC genes at two loci and demonstration of physical linkage. Immunogenetics 33: 247-254 (1991)

23) Horst, E., Wijngaard, P.L.J., Metzelaar, M., Bast, E.J.E.G., and Clevers, H.C.C. A method for cDNA cloning in COS cells irrespective of subcellular site of expression. Nucleic Acids Res. 19: 4556 (1991)

24) Oosterwegel, M.A., van de Wetering, M.L., Holstege, F.C.P., Prosser, H.M., Owen, M.J., and Clevers, H.C. TCF-1, a T cell-specific transcription factor of the HMG box family, interacts with sequence motifs in the TCR beta and TCR delta enhancers. Int.Immunol. 3: 1189-1192 (1991)

25) Castrop, J., van Norren, K., and Clevers, H.C. A gene family of HMG box factors with homology to TCF-1. Nucleic Acids Res. 20: 611 (1992)

26) Castrop, J., Hoevenagel, R., and Clevers, H.C. A common ancestor of the mammalian transcription factors TCF-1 and TCF-1 alpha/LEF1 expressed in chicken T cells. Eur.J.Immunol. 22: 1327-1330 (1992)

27) van de Wetering, M., and Clevers, H.C. Sequence-specific interaction of the HMG-box factor TCF-1 occurs within the minor groove of a Watson-Crick double helix. EMBO J. 11: 3039-3044 (1992)

28) Metzelaar, M.J., and Clevers, H.C. Lysosomal membrane glycoproteins in platelets. Thrombosis and Haemostasis 68: 378-382 (1992)

29) Wijngaard, P.L.J., Metzelaar, M., MacHugh, N.D., Morrison, W.I., and Clevers, H.C. Molecular characterisation of the WC1 antigen expressed specifically on bovine CD4-CD8- gamma delta T-lymphocytes. J.Immunol. 149: 3273-3277 (1992)

30) van de Wetering, M., Oosterwegel, M., Holstege, F., Suykerbuyk, R., Geurts van Kessel, A., and Clevers, H.C. Structure and mapping of the human TCF-1 gene; Characterization of its promoter. J.Biol.Chem. 267: 8530-8536 (1992)

31) Oosterwegel, M.A., Timmerman, J., Leiden, J., and Clevers, H.C. Expression of GATA-3 during lymphocyte differentiation and mouse embryogenesis. Developmental Immunology 3:1-11 (1992)

32) Demetrick, D., Herlyn, D., Tretiak, M., Creasey D., Clevers, H.C., Vennegoor, C.J.G.M., Dixon, W.T., and Jerry, L.M. The ME491 melanoma-associated glycoprotein family. Antigenic identity of

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the ME491, NKI/C-3, NGA and CD61 proteins. J.Nat.Cancer Inst. 84: 422-429 (1992)

33) de Lau, W.B.M., Boom, S.E., Heije K., Griffioen, A.W., Braakman, E., Bolhuis, R.L.H., Tax, W.J.M., Clevers, H., and Bast E.J.E.G. Heterodimeric complex formation with CD8 and TCR by bispecific antibody sustains paracrine IL-2-dependent growth of CD3+ CD8+ T cells. J. Immunol. 149:1840-1846 (1992)

34) Schuurman, H-J., Hu, H., de Weger, R.A., and Clevers, H.C. Thoughts on thymus and T-lymphocyte repertoire. Relevance to the tolerance of the immune response. Neth.J.Med. 43: 38-54 (1993)

35) Oosterwegel, M., van de Wetering, M., Timmerman, J., Kruisbeek, A., Destree, O., Meijlink, F., and Clevers, H.C. Differential expression of the HMG box factors TCF-1 and LEF-1 during murine embryogenesis. Development 118: 439-448 (1993)

36) Clevers, H.C. De rol van transcriptiefactoren in de differentiatie van beenmergstamcellen naar immuuncompetente T lymfocyten. Mediator, juni/juli (1993)

37) Laudet, V., Stehelin, D., and Clevers, H.C. Ancestry and diversity of the HMG box superfamily. Nucleic Acids Res. 21: 2493-2501 (1993)

38) Schilham, M.W., van Eijk, M., van de Wetering, M., and Clevers, H.C. The murine Sox-4 protein is encoded on a single exon. Nucleic Acids Res. 21: 2009-2009 (1993)

39) van de Wetering, M., and Clevers, H.C. Sox 15, a novel member of the murine Sox family of HMG box transcription factors. Nucleic Acids Res. 21: 1669-1669 (1993)

40) de Lau, W.B.M., Kuipers, J., Voshol, H., Clevers, H.C., and Bast, E.J.E.G. The HB4 monoclonal antibody recognizes a carbohydrate structure on lymphocyte surface proteins, related to HB6, CDw75 and CD76. J.Immunol. 150: 4911-1919 (1993)

41) van de Wetering, M., Oosterwegel, M., van Norren, K. and Clevers, H.C. SOX-4, an SRY-like HMG box protein, is a transcriptional activator in lymphocytes. EMBO J. 12: 3847-3854 (1993)

42) van Houte, L., van Oers, A.M., van de Wetering, M., Dooijes, D., Kaptein, R., and Clevers, H. The sequence-specific HMG box of TCF-1 adopts a predominantly alpha-helical conformation in solution. J.Biol.Chem. 268: 18083-18087 (1993)

43) Oosterwegel, M., van de Wetering, M. and Clevers, H.C. HMG box proteins in early T cell differentiation. Thymus 22: 67-81 (1993)

44) Clevers, H.C., Oosterwegel, M.A., and Georgopoulos, K. Transcription factors in early T cell development. Immunology Today 14: 591-596 (1993)

45) Dooijes, D., van de Wetering, M., Knippels, L. and Clevers, H.C. The Schizosaccharomyces pombe mating-type gene mat-MC encodes a sequence-specific DNA-binding HMG box protein. J. Biol.Chem. 268: 24813-24817 (1993)

46) MacHugh, N.D., Wijngaard, P.L.J., Clevers, H.C., and Davis, W.C. Clustering of monoclonal-antibodies recognizing different members of the WC1 gene family. Vet. Immunol. 39: 155-160 (1993)

47) Wijngaard, P.L.J., MacHugh, N.D., Metzelaar, M.J., Romberg, S., Bensaid, A., Pepin, L, Davis, W.C. and Clevers, H.C. Members of the novel WC1 gene family are differentially expressed on subsets of bovine CD4-CD8- gamma delta T-lymphocytes. J.Immunol. 152: 3476-3482 (1994)

48) Fournier, B., Saudubray, J-M., Benichou, B., Lyonnet, S., Munnich, A., Clevers, H.C. and Poll-The, B.T. Large deletion of the peroxisomal acyl-CoA oxidase gene in pseudoneonatal

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adrenoleukodystrophy. J.Clin.Invest. 94: 526-531 (1994)

49) Walker, I.D., Glew, M.D., O'Keeffe, M.A., Metcalfe, S.A., Clevers, H.C., Wijngaard, P.L., Adams, T.E. and Hein, W.R. A novel multi-gene family of sheep gamma delta T cells. Immunology 83: 517-523 (1994)

50) Verbeek, J.S., Ison, D., Hofhuis, F., Robanus-Maandag, E., te Riele, H., van de Wetering, M., Oosterwegel, M., Wilson, A., MacDonald, H.R. and Clevers, H.C. An HMG box containing T-cell factor required for thymocyte differentiation. Nature 374: 70-74 (1995)

51) Toye, P.G., Metzelaar, M.J., Wijngaard, P.L.J., Nene, V., Iams, K., Roose, J., Nyanjui, J.K., Gobright, E., Musoke, A.J. and Clevers, H.C. Characterization of the gene encoding the polymorphic immunodominant molecule, a neutralizing antigen of Theileria-parva. J. Immunol. 155:1370-1381 (1995)

52) Castrop., J., Verbeek, S., Hofhuis, F., and Clevers, H. Circumvention of tolerance for the nuclear T cell protein Tcf-1 by immunization of Tcf-1 knock-out mice. Immunobiology 193: 281-287 (1995)

53) van Houte, L.P.A., Chuprina, V.P., van de Wetering, M., Boelens, R., Kaptein, R. and Clevers, H. Solution structure of the sequence-specific HMG box of the lymphocyte transcriptional activator SOX-4. J.Biol. Chem. 270: 30516-30524 (1995)

54) Castrop, J., van Wichen, D., Koomans-Bitter, M., van de Wetering, M., de Weger, R., van Dongen, J., and Clevers, H.. The human Tcf-1 gene encodes a nuclear DNA-binding protein, uniquely expressed in normal and neoplastic T lineage lymphocytes. Blood 86: 3050-3059 (1995)

55) Scheffer, G.L., Wijngaard, P.L.J., Flens, M.J., Izquierdo, M.A., Slovak, M.L., Pinedo, H.M., Meijer, C.J.L.M., Clevers, H.C. and Scheper, R.J. The drug-resistance-related protein LRP is the human major vault protein. Nature Medicine 1: 578-582 (1995)

56) Mayer, K., Wolff, E., Clevers, H. and Ballhausen, W.G. The HMG box transcription factor TCF-1: novel isoforms due to alternative splicing and usage of a new exon IXA. Biochim. Biophys. Acta 1263: 169-172 (1995)

57) Kamoun, M., Woods, J.S., et al., and Clevers, H. Analysis of CD2 and TCR-beta gene expression in Jurkat cell mutants suggests a cis regulation of gene expression. J. Immunol. 155: 3929-3937 (1995)

58) Toye, P., Wijngaard, P., MacHugh, N., and Clevers, H. An assay for the identification of antigens recognized by cytotoxic T-cells, based on transient transfection of COS cells. J. Immunol. Methods 187: 95-101 (1995)

59) Ohteki, T., Wilson, A., Verbeek, S., MacDonald, H.R., and Clevers, H. Selectively impaired development of intestinal T cell receptor gamma delta+ cells and liver CD4+ NK1+ T cell receptor alpha beta+ cells in T cell factor-1-deficient mice. Eur. J. Immunol. 26: 351-355 (1996)

60) van de Wetering, M., Castrop, J., Korinek, V., and Clevers, H. Extensive alternative splicing and dual promoter usage generates TCF-1 protein isoforms with differential transcription control properties. Mol. Cell. Biol. 16: 745-752 (1996)

61) Schilham, M., Oosterwegel, M., Moerer, P., Jing Ya, de Boer, P., van de Wetering, M., Verbeek, S., S., Lamers, W., Kruisbeek, A., Cumano, A., and Clevers, H. Sox-4 gene is required for cardiac outflow tract formation and pro-B lymphocyte expansion. Nature 380: 711-714 (1996)

62) Clevers, H., and Grosschedl, R. Transcriptional control of lymphoid development. Lessons from gene targeting. Immunology Today 17: 336-343 (1996)

63) Molenaar, M., Van de Wetering, M., Oosterwegel, M., Peterson-Maduro, J., Godsave, S., Korinek, V., Roose, J., Destrée, O. And Clevers, H. Xtcf-3 Transcription factor mediates beta-

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catenin-induced axis formation in xenopus embryos. Cell 86, 391-399 (1996)

64) Petersen, E.J., Rozenberg-Arska, M., Dekker, A.W., Clevers, H.C., and Verdonck, L.F. Allogeneic bone marrow transplantation can restore CD4+ T-lymphocyte count and immune function in idiopathic CD4+ T-lymphocytepenia. Bone marrow transplantation 18: 813-815 (1996)

65) Santos, M., Schilham, M.W., Rademakers, L.P.M., Marx, J.J.M., de Sousa, M., and Clevers, H. Defective iron homeostasis in beta 2-microglobulin knockout mice recapitulates hereditary hemochromatosis in man. J. Exp. Med. 184: 1975-1985 (1996)

66) Korinek, V., Barker, N., Morin, P.J., van Wichen, D., de Weger, R., Kinzler, K.W., Vogelstein, B., and Clevers, H. Constitutive Transcriptional Activation by a beta-catenin-Tcf complex in APC-/- Colon Carcinoma. Science 275: 1784-1787 (1997)

67) Morin, P.J., Sparks, A., Korinek, V., Barker, N., Clevers, H., Vogelstein, B., and Kinzler, K. Activation of beta-catenin-Tcf signaling in colon cancer by mutations in beta-catenin or APC. Science 275: 1787-1790 (1997)

68) van de Wetering, M., Cavallo, R., Dooijes, D., van Beest, M., van Es, J., Loureiro, J., Ypma, A., Hursh, D., Jones, T., Bejsovec, A., Peifer, M., Mortin, M., and Clevers, H. Armadillo co-activates transcription driven by the product of the Drosophila segment polarity gene dTCF. Cell 88, 789-799 (1997)

69) Mayer, K., Hieronymus, T., Castrop, J., Clevers, H. and Ballhausen, W.G. Ectopic activation of lymphoid high mobility group-box transcription factor TCF-1 and overexpression in colorectal cancer cells. Int. J. Cancer 72: 625-630 (1997)

70) Ya, J., Schilham, M.W., Clevers, H.C., Moorman, A.F. and Lamers, W.H. Animal models of congenital defects in the ventriculoarterial connection of the heart. J. Mol. Med. 75: 551-566 (1997)

71) Clevers, H., and van de Wetering, M. TCF/LEF factors earn their wings. Trends in Genetics 13: 485-489 (1997)

72) Santos, M., Clevers, H.C. and Marx J.J. Mutations of the hereditary hemochromatosis candidate gene HLA-H in porphyria cutanea tarda. N. Eng. J. Med. 336: 1327-1328 (1997)

73) Schilham, M.W., Moerer, P., Cuomo, A. and Clevers, H.C. Sox-4 facilitates thymocyte differentiation. Eur. J. Immunol. 27: 1292-1295 (1997)

74) Kjaerulff, S., Dooijes, D., Clevers, H. and Nielsen, O. Cell differentiation by interaction of two HMG-box proteins: Mat1-Mc activates M cell-specific genes in S.pombe by recruiting the ubiquitous transcription factor Ste11 to weak binding sites. EMBO J. 16: 4021-4033 (1997)

75) Dooijes, D. and Clevers, H. Control of gene expression during lymphoid development: targeted gene disruption provides new clues. APMIS 105: 337-344 (1997)

76) Clevers, H. and Miedema, F. European immunologists meet in Amsterdam. Immunology Today 18, 257 (1997)

77) Santos, M., Wienk, K.J., Schilham, M.W., Clevers, H., de Sousa, M. and Marx, J.J. In vivo mucosal uptake, mucosal transfer and retention of iron in mice. Lab. Anim. 31: 264-270 (1997)

78) Daubenberger, C., Heussler, V., Gobright, E., Wijngaard, P., Clevers, H.C., Wells, C., Tsuji, N., Musoke A. and McKeever, D. Molecular characterisation of a cognate 70 kDa heat shock protein of the protozoan Theileria parva. Mol. Biochem. Parasitol. 85: 265-269 (1997)

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79) Korver, W., Roose, J. and Clevers, H. The winged-helix transcription factor Trident is expressed in cycling cells. Nucleid Acids Res. 25: 1715-1719 (1997)

80) Korver, W., Roose, J., Wilson, A. and Clevers, H. The winged-helix transcription factor Trident is expressed in actively dividing lymphocytes. Immunobiol. 198: 157-161 (1997)

81) Korver, W., Roose, J., Heinen, K., Olde Weghuis, D., De Bruijn, D., Geurts van Kessel, A. and Clevers, H. The human TRIDENT/HFH-11/FKHL16 gene: structure, localization and promoter characterization. Genomics 46: 435-442 (1997)

82) Korinek, V., Barker, N., Willert, K., Molenaar, M., Roose, J., Wagenaar, G., Markman, M., Lamers, W., Destree, O. and Clevers, H. Two members of the Tcf family implicated in Wnt/beta-catenin signaling during embryogenesis in the mouse. Mol. Cell Biol. 18: 1248-1256 (1998)

83) Roose, J., Korver, W., Oving, E., Wilson, A., Wagenaar, G., Markman, M., Lamers, W. and Clevers, H. High expression of the HMG box factor sox-13 in arterial walls during embryonic development. Nucleic Acids Res. 26: 469-476 (1998)

84) Okamura, R.M., Sigvardsson, M., Galceran, J., Verbeek, S., Clevers, H. and Grosschedl, R. Redundant regulation of T cell differentiation and TCRalpha gene expression by the transcription factors LEF-1 and TCF-1. Immunity 8: 11-20 (1998)

85) Schilham, M.W., and Clevers, H. HMG box containing transcription factors in lymphocyte differentiation.Semin. Immunology 10: 127-132 (1998)

86) de Lau, W.B.M., Hurenkamp, J., Berendes, P., Touw, I.P., Clevers, H.C., and van Dijk, M.A. The gene encoding the granulocyte colony-stimulating factor receptor is a target for deregulation in pre-B ALL by the t(1;19)-specific oncoprotein E2A-Pbx1. Oncogene 17: 503-510 (1998)

87) de Lau, W.B., Kuipers, J., Peters, P.J., Lokhorst, H.M., Clevers, H. and Bast, B.J. Putative myeloma precursor cells expressing 2,6 sialic acid modified antigens actually belong to the erythroid lineage. Leuk Res 22: 163 173 (1998)

88) Clevers, H. and Ferrier, P. Transcriptional control during T cell development. Curr Opin Immunol 10: 166 171 (1998)

89) Dooijes, D., van Beest, M., van de Wetering, M., Boulanger, G., Jones, T., Clevers, H. and Mortin, M.A. Genomic organization of the segment polarity gene pan in Drosophila melanogaster. Mol Gen Genet 258: 45 52 (1998)

90) Santos, M., Clevers, H., de Sousa, M. and Marx, J.J. Adaptive response of iron absorption to anemia, increased erythropoiesis, iron deficiency, and iron loading in beta2 microglobulin knockout mice. Blood 91: 3059 3065 (1998)

91) Molenaar, M., Roose, J., Peterson, J., Venanzi, S., Clevers, H. and Destree, O. Differential expression of the HMG box transcription factors XTcf 3 and Xlef 1 during early Xenopus development. Mech Dev 75: 163 166 (1998)

92) Korinek, V., Barker, N., Moerer, P., van Donselaar, E., Huls, G., Peters, P.J. and Clevers, H. Depletion of epithelial stem cell compartments in the small intestine of mice lacking Tcf 4. Nat Genet 19: 379 383 (1998)

93) van Beest, M., Mortin, M. and Clevers, H. Drosophila RpS3a, a novel Minute gene situated between the segment polarity genescubitus interruptus and dTCF. Nucleic Acids Res 26: 4471 4475 (1998)

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94) Roose, J., Molenaar, M., Peterson, J., Hurenkamp, J., Brantjes, H., Moerer, P., van de Wetering, M., Destree, O., and Clevers, H. The Xenopus Wnt effector XTcf-3 interacts with Groucho-related transcriptional repressors. Nature 395: 608-612 (1998)

95) Cavallo, R.A., Cox, R.T., Moline, M.M., Roose, J., Polevoy, G.A., Clevers, H., Peifer, M., and Bejsovec A. Drosophila Tcf and Groucho interact to repress Wingless signalling activity. Nature 395: 604-608 (1998)

96) Zhang, Z., Hartmann, H., Minh Do, V., Abramowski, D., Sturchler-Pierrat, C., Staufenbiel, M., Sommer, B., van de Wetering, M., Clevers, H., Saftig, P., De Strooper, B., He, X. and Yankner, B.A. Destabilization of beta-Catenin by Presenilin-1 Mutations Increases Neuronal Vulnerability to Apoptosis. Nature 395: 698-702 (1998)

97) Schilham, M.W., Wilson, A., Moerer, P., Benaissa-Trouw, B.J., Cumano, A., Clevers, H.C. Critical involvement of Tcf-1 in expansion of thymocytes. J. Immunol 161: 3984-3991 (1998)

98) Ya, J., Schilham, M.W., de Boer, P.A., Moorman, A.F., Clevers, H., Lamers, W.H. Sox4-deficiency syndrome in mice is an animal model for common trunk. Circ. Res 83: 986-994 (1998)

99) Korver, W., Schilham, M.W., Moerer, P., van den Hoff, M.J., Dam, K., Lamers, W.H., Medema, R.H., Clevers, H. Uncoupling of S phase and mitosis in cardiomyocytes and hepatocytes lacking the winged-helix transcription factor Trident. Curr. Biol. 8: 1327-1330 (1998)

100) van Es, J.H., Kirkpatrick, C., van de Wetering, M., Molenaar, M., Miles, A., Kuiper, J., Destree,O., and Clevers, H. Identification of APC2, a homologue of the adenomatous polyposis coli tumour suppressor. Curr. Biol. 9: 105-108 (1999)

101) Meyaard, L., Hurenkamp, J., Clevers, H., Lanier, L.L., Phillips, J.H. Leukocyte-Associated Ig-Like Receptor-1 Functions as an Inhibitory Receptor on Cytotoxic T Cells. J. Immunol 162: 5800-5804 (1999)

102) Staal, F.J.T, Burgering, B.M.T., van de Wetering, M., and Clevers, H. Tcf-1-mediated transcription in T lymphocytes: differential role for GSK3beta in fibroblasts and T cells. Int Immunol 11: 317-322 (1999)

103) Roose, J., Huls, G., van Beest, M., Moerer, P., van der Horn, K., Goldschmeding, R., Logtenberg, T., and Clevers, H. Synergie between tumor suppressor APC and the beta-catenin/Tcf4 target gene Tcf1. Science 285: 1923-1926 (1999)

104) Ishitani, T., Ninomiya-Tsuji, J., Nagai, S., Nishita, M., Meneghini, M., Barker, N., Waterman, M., Bowerman, B., Clevers, H., Shibuya, H., Matsumoto, K. The TAK1-NLK-MAPK-related pathway antagonizes signalling between beta-catenin and transcription factor TCF. Nature 399: 798-802 (1999)

105) Roose, J., Clevers, H. TCF transcription factors: molecular switches in carcinogenesis. Biochim. Biophys. Acta 1424: M23-M37 (1999)

106) van der Vuurst de Vries, A.R., Clevers, H., Logtenberg, T., Meyaard, L. Leukocyte-associated immunoglobulin-like receptor-1 (LAIR-1) is differentially expressed during human B cell differentiation and inhibits B cell receptor-mediated signaling. Eur. J. Immunol. 29: 3160-3167 (1999)

107) Barker, N., Huls, G., Korinek, V., Clevers, H. Restricted high level expression of Tcf-4 protein in intestinal and mammary gland epithelium. Am J Pathol 154: 29-35 (1999)

108) Galceran, J., Farinas, I., Depew, M.J., Clevers, H., Grosschedl, R. Wnt3a-/--like phenotype and limb deficiency in Lef1(-/-)Tcf1(-/-) mice. Genes Dev 13: 709-717 (1999)

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109) Held, W., Kunz, B., Lowin-Kropf, B., van de Wetering, M., Clevers, H. Clonal acquisition of the Ly49A NK cell receptor is dependent on the trans-acting factor TCF-1. Immunity 11: 433-442 (1999)

110) Kuipers, J., Vaandrager, J.W., Weghuis, D.O., Pearson, P.L., Scheres, J., Lokhorst, H.M., Clevers, H., Bast, B.J. Fluorescence in situ hybridization analysis shows the frequent occurrence of 14q32.3 rearrangements with involvement of immunoglobulin switch regions in myeloma cell lines. Cancer Genet Cytogenet 109: 99-107 (1999)

111) Roose, J., Korver, W., de Boer, R., Kuipers, J., Hurenkamp, J., Clevers, H. The Sox-13 gene: structure, promoter characterization, and chromosomal localization. Genomics 57: 301-305 (1999)

112) Wielenga, V.J., Smits, R., Korinek, V., Smit, L., Kielman, M., Fodde, R., Clevers, H., Pals, S.T. Expression of CD44 in Apc and Tcf mutant mice implies regulation by the WNT pathway. Am J Pathol 54: 515-523 (1999)

113) Korswagen, H.C., Clevers, H.C. Activation and repression of wingless/Wnt target genes by the TCF/LEF-1 family of transcription factors. Review. Cold Spring Harb Symp Quant Biol. 64: 141-147 (1999)

114) Barker, N., Morin, P.J., Clevers, H. The Yin-Yang of TCF/b-Catenin Signaling. Advances in Cancer Research Volume 77: 1-24 (2000)

115) Bienz, M., and Clevers, H. Linking Colorectal Cancer to Wnt Signaling. Cell 103:311-320 (2000)

116) Korswagen, R., Herman, M., and Clevers, H. Distinct beta-catenins mediate adhesion and signalling functions in C. elegans. Nature 406: 527-532 (2000)

117) Lickert, H., Domon, C., Huls, G., Wehrle, C., Duluc, I., Clevers, H., Meyer, B.I., Freund, J-N., and Kemler, R. Wnt/beta-catenin signaling regulates the expression of the homeobox gene Cdx1 in embryonic intestine. Development 127:3805-3813 (2000)

118) Clevers, H. Armadillo takes the APC shuttle. Nature cell biology (news and views) E177-E178 (2000)

119) Yang, X., van Beest, M., Clevers, H., Jones, T., Hursh, D.A., Mortin, M.A. Decapentaplegic is a direct target of dTcf repression in the Drosophila visceral mesoderm. Development 127:3695-3702 (2000)

120) Barker, N., Clevers, H. Catenins, Wnt signaling and cancer. Bioessays 22: 961-965 (2000) 121) Cheung, M., Abu-Elmagd, M., Clevers, H., Scotting, P.J. Roles of Sox4 in central nervous system

development. Brain Res Mol Brain Res 79: 180-191 (2000)

122) van Beest, M., Dooijes, D., van de Wetering, M., Kjearulff, S., Bonvin, A., Nielsen, O., Clevers, H. Sequence-specific high mobility group box factors recognize 10-12-base pair minor groove motifs. J Biol Chem 275: 27266-27273 (2000)

123) Molenaar, M., Brian, E., Roose, J., Clevers, H., Destree, O. Differential expression of the Groucho-related genes 4 and 5 during early development of Xenopus laevis. Mech Dev 91: 311-315 (2000)

124) Clevers, H. Axin and hepatocellular carcinomas. Nat Genet 24: 206-208 (2000)

125) Lawrence, N., Dearden, P., Hartley, D., Roose, J., Clevers, H., Arias, A.M. dTcf antagonises Wingless signalling during the development and patterning of the wing in Drosophila. Int J Dev Biol 44: 749-756 (2000)

126) Santos, M.M., de Sousa, M., Rademakers, L.H., Clevers, H., Marx, J.J., Schilham, M.W. Iron overload and heart fibrosis in mice deficient for both beta2-microglobulin and Rag 1. Am J Pathol 157: 1883-1892 (2000)

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127) Staal, F.J.T., Clevers, H. Tcf/Lef transcription factors during T-cell development: unique and overlapping functions. The Hematology Journal 1: 3-6 (2001)

128) Staal, F.J.T., Meeldijk, J., Moerer, P., Jay, P., van de Weerdt, B.C.M., Vainio, S., Nolan, G.P., Clevers, H. Wnt signaling is required for thymocyte development and activates Tcf-1 mediated transcription. Eur J Immunol, 31: 285-293 (2001)

129) Fodde, R., Kuipers, J., Rosenberg, C., Smits, R., Kielman, M., Gaspar, C., van Es, J.H., Breukel, C., Wiegant, J., Giles, R.H. and Clevers, H. Mutations in the APC tumor suppressor gene cause chromosomal instability in colorectal cancer. Nature Cell Biology 3: 433-438, (2001)

130) Staal, F.J.T., Weerkamp, F., Langerak, A.W., Hendriks, R.W., Clevers, H.C., Transcriptional Control of T Lymphocyte Differentiation. Stem Cells 19: 165-179 (2001)

131) Ioannidis,V., Beermann, F., Clevers, H., Held, W. The beta-catenin--TCF-1 pathway ensures CD4(+)CD8(+) thymocyte survival. Nat Immunol. 2: 691-697 (2001)

132) Staal, F.J., Clevers, H.C., Regulation of lineage commitment during lymphocyte development. Review. Int Rev Immunol. 20: 45-64 (2001)

133) de Lau, W., Clevers, H. LEF1 turns over a new leaf. Nat Genet. 28: 3-4 (2001)

134) Brantjes, H., Roose, J., van De Wetering, M., Clevers, H. All Tcf HMG box transcription factors interact with Groucho-related co-repressors. Nucleic Acids Res. 29: 1410-1419 (2001)

135) van Es, J.H., Giles, R.H., Clevers, H.C. The many faces of the tumor suppressor gene APC. Review Exp Cell Res. 264: 126-134 (2001)

136) van de Wetering, M., Barker, N., Harkes, I.C., van der Heyden, M., Dijk, N.J., Hollestelle, A., Klijn, J.G., Clevers, H., Schutte, M. Mutant E-cadherin breast cancer cells do not display constitutive Wnt signaling. Cancer Res. 61: 278-284 (2001)

137) van de Water, S., van de Wetering, M., Joore, J., Esseling, J., Bink, R., Clevers, H., Zivkovic, D. Ectopic Wnt signal determines the eyeless phenotype of zebrafish masterblind mutant. Development 128: 3877-3888 (2001)

138) Barker, N., Clevers, H. Tumor environment: a potent driving force in colorectal cancer? Trends Mol Med 7: 535-537 (2001)

139) Barker, N., Hurlstone, A., Musisi, H., Miles, A., Bienz, M., Clevers, H. The chromatin remodelling factor Brg-1 interacts with beta-catenin to promote target gene activation. EMBO J 20: 4935-4943 (2001)

140) Fodde, R., Smits R., Clevers, H. APC, signal transduction and genetic instability in colorectal cancer. Review. Nat Rev Cancer 1: 55-67 (2001)

141) van Noort, M., Meeldijk, J., van der Zee, R., Destree, O., Clevers, H. Wnt signaling controls the phosphorylation status of beta-catenin. J Biol Chem 277: 17901-17905 (2002)

142) Staal, F.J., van Noort, M., Strous, G.J., Clevers, H.C. Wnt signals are transmitted through N-terminally dephosphorylated {beta}-catenin. EMBO Rep 3: 63-68 (2002)

143) Lustig, B., Jerchow, B., Sachs, M, Weiler, S., Pietsch, T., Karsten, U., van de Wetering, M., Clevers, H., Schlag, P.M., Birchmeier, W., Behrens, J. Negative feedback loop of Wnt ignaling through upregulation of conductin/axin2 in colorectal and liver tumors. Mol Cell Biol. 22: 1184-1193 (2002)

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144) Brantjes, H., Barker, N., van Es, J., Clevers, H. TCF: Lady Justice casting the final verdict on the outcome of Wnt signalling. Review. Biol Chem. 383: 255-261 (2002)

145) van de Wetering, M., de Lau, W., Clevers, H. WNT signaling and lymphocyte development. Review. Cell 109 Suppl: S13-19 (2002)

146) van Noort, M., Clevers, H. TCF transcription factors, mediators of Wnt-signaling in development and cancer. Dev Biol. 244: 1-8 (2002)

147) Korswagen, H.C., Coudreuse, D.Y., Betist, M.C., van de Water, S., Zivkovic, D., Clevers, H.C. The Axin-like protein PRY-1 is a negative regulator of a canonical Wnt pathway in C. elegans. Genes Dev. 16: 1291-1302 (2002)

148) Hurlstone A., Clevers H. T-cell factors: turn-ons and turn-offs. EMBO J. 21: 2303-2311 (2002) 149) Hurlstone, A.F., Olave, I.A., Barker, N., van Noort, M., Clevers H. Cloning and characterization of

hELD/OSA1, a novel BRG1 interacting protein. Biochem J. 364(Pt 1):255-264 (2002)

150) Cheon, S.S., Cheah, A.Y., Turley, S., Nadesan, P., Poon, R., Clevers, H., Alman, B.A. beta-Catenin stabilization dysregulates mesenchymal cell proliferation, motility, and invasiveness and causes aggressive fibromatosis and hyperplastic cutaneous wounds. Proc Natl Acad Sci U S A. 99: 6973-6978 (2002)

151) van Noort, M., van de Wetering, M., Clevers, H. Identification of two novel regulated serines in the N terminus of beta-catenin. Exp Cell Res. 276: 264-272 (2002)

152) Oving, I.M., Clevers, H.C. Molecular causes of colon cancer. Eur J Clin Invest 32: 448-457 (2002) 153) Boon, E.M., van der Neut, R., van de Wetering, M., Clevers, H., Pals, S.T. Wnt signaling

regulates expression of the receptor tyrosine kinase met in colorectal cancer. Cancer Res 62: 5126-5128 (2002)

154) Gallagher, R.C., Hay, T., Meniel, V., Naughton, C., Anderson, T.J., Shibata H, Ito M, Clevers, H., Noda, T., Sansom, O.J., Mason J.O., Clarke, A.R. Inactivation of Apc perturbs mammary development, but only directly results in acanthoma in the context of Tcf-1 deficiency. Oncogene 21: 6446-6457 (2002)

155) Batlle, E., Henderson, J.T., Beghtel, H., van den Born, M., Sancho, E., Huls, G., Meeldijk, J., Robertson, J., van de Wetering, M., Pawson, T., Clevers, H. Beta- catenin and TCF mediate cell positioning in the intestinal epithelium by controlling the expression of EphB/ephrinB. Cell 111: 251-263 (2002)

156) Van de Wetering, M., Sancho, E., Verweij, C., de Lau, W., Oving, I., Hurlstone, A., van der Horn, K., Batlle, E., Coudreuse, D., Haramis, A-P., Tjon-Pon-Fong, M., Moerer, P., van den Born, M., Soete, G., Pals, S., Eilers, M., Medema, R., Clevers, H. The beta catenin/TCF4 complex imposes a crypt progenitor phenotype on colorectal cancer cells. Cell 111: 241-250 (2002)

157) Clevers, H. Inflating cell numbers by Wnt. Mol Cell 10: 1260-1261 (2002)

158) van Es, J.H., Barker, N., Clevers, H. You Wnt some, you lose some: oncogenes in the Wnt signaling pathway. Curr Opin Genet Dev 13: 28-33 (2003)

159) Bienz, M., Clevers, H. Armadillo/beta-catenin signals in the nucleus--proof beyond a reasonable doubt? Nat Cell Biol 5: 179-182 (2003)

160) Nicolas, M., Wolfer, A., Raj, K., Kummer, J.A., Mill, P., van Noort, M., Hui, C.C., Clevers, H., Dotto, G.P., Radtke, F. Notch1 functions as a tumor suppressor in mouse skin. Nat Genet 33: 416-421 (2003)

161) Staal, F.J., Clevers, H.C. Wnt signaling in the thymus. Curr Opin Immunol 15: 204-208 (2003)

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162) Held, W., Clevers, H., Grosschedl, R. Redundant functions of TCF-1 and LEF-1 during T and NK cell development, but unique role of TCF-1 for Ly49 NK cell receptor acquisition. Eur J Immunol 33: 1393-1398 (2003)

163) Van De Wetering, M., Oving, I., Muncan, V., Pon Fong, M.T., Brantjes, H., Van Leenen, D., Holstege, F.C., Brummelkamp, T.R., Agami, R., Clevers, H. Specific inhibition of gene expression using a stably integrated, inducible small-interfering-RNA vector. EMBO Rep 4:609-615 (2003)

164) Giles, R.H., van Es, J.H., Clevers, H. Caught up in a Wnt storm: Wnt signaling in cancer. Review Biochim Biophys Acta. 1653: 1-24 (2003)

165) Baas, A.F., Boudeau, J., Sapkota, G.P., Smit, L., Medema, R.,Morrice, N.A., Alessi, D.R., Clevers, H.C. Activation of the tumor suppressor kinase LKB1 by the STE20-like pseudokinase STRAD. EMBO J. 22: 3062-3072 (2003)

166) Pinto D, Gregorieff A, Begthel H, Clevers H. Canonical Wnt signals are essential for homeostasis of the intestinal epithelium. Genes Dev. 17: 1709-1713 (2003)

167) Kim, P.J., Plescia, J., Clevers, H. Fearon E.R., Altieri D.C. Survivin and molecular pathogenesis of colorectal cancer. Lancet 362: 205-209 (2003)

168) Brinkmeier, M.L., Potok, M.A., Cha, K.B., Gridley, T., Stifani, S., Meeldijk, J., Clevers, H. Camper SA. TCF4 and groucho-related genes influence pituitary growth and development. Mol Endocrinol. 17: 2152-2161 (2003)

169) Clevers, H. Thymocyte development: drama without Brahma. Immunity 19: 157-158 (2003)

170) Boudeau, J., Baas, A.F., Deak, M., Morrice, N.A., Kieloch, A., Schutkowski, M., Prescott, A.R., Clevers, H.C., Alessi, D.R. MO25alpha/beta interact with STRADalpha/beta enhancing their ability to bind, activate and localise LKB1 in the cytoplasm. EMBO J. 22: 5102-5114 (2003)

171) Hurlstone, A.F., Haramis, A.P., Wienholds, E., Begthel, H., Korving, J., Van Eeden, F., Cuppen, E., Zivkovic, D., Plasterk, R.H., Clevers, H. The Wnt/beta-catenin pathway regulates cardiac valve formation. Nature 425: 633-637 (2003)

172) Sancho, E., Batlle, E., Clevers H. Live and let die in the intestinal epithelium. Curr Opin Cell Biol. 15: 763-770 (2003)

173) Morkel, M., Huelsken, J., Wakamiya, M., Ding, J., van de Wetering, M., Clevers, H., Taketo, M.M., Behringer, R.R., Shen, M.M., Birchmeier, W. Beta-catenin regulates Cripto- and Wnt3-dependent gene expression programs in mouse axis and mesoderm formation. Development 130: 6283-6294 (2003)

174) Verbrugge, A., Ruiter, Td. T., Clevers, H. Meyaard, L. Differential contribution of the immunoreceptor tyrosine-based inhibitory motifs of human leukocyte-associated Ig-like receptor-1 to inhibitory function and phosphatase recruitment. Int Immunol. 15: 1349-1358 (2003)

175) Baas, A.F., Kuipers, J., van der Wel, N.N., Batlle, E., Koerten, H.K., Peters, P.J., Clevers, H.C. Complete polarization of single intestinal epithelial cells upon activation of LKB1 by STRAD. Cell 116: 457-466 (2004)

176) Haramis, A.P., Begthel, H., van den Born, M., van Es, J., Jonkheer, S., Offerhaus G.J., Clevers H. De novo crypt formation and Juvenile Polyposis upon BMP inhibition. Science 303: 1684-1686 (2004)

177) Smit, L., Baas, A., Kuipers, J., Korswagen, H., van De Wetering, M., Clevers H. Wnt activates the Tak1/Nlk kinase pathway. J Biol Chem. 279: 17232-17240 (2004)

178) Clevers, H. Wnt breakers in colon cancer. review Cancer Cell 5: 5-6 (2004)

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179) Gregorieff, A., Grosschedl, R., Clevers, H. Hingut defects and transformation of the gastro-intestinal tract in Tcf4-/-/Tcf1-/- embryos. EMBO J. 23: 1825-1833 (2004)

180) Derksen, P.W., Tjin, E., Meijer, H.P., Klok, M.D., MacGillavry, H.D., van Oers, M.H., Lokhorst, H.M., Bloem, A.C., Clevers, H., Nusse, R, van der Neut, R., Spaargaren, M., Pals, S.T. Illegitimate WNT signaling promotes proliferation of multiple myeloma cells. Proc Natl Acad Sci U S A. 101: 6122-6127 (2004)

181) Le, N.H., Van Der Bent, P., Huls, G., Van De Wetering, M., Loghman-Adham, M., Ong, A.C., Calvet, J.P., Clevers, H., Breuning, M.H., Van Dam, H., Peters, D.J. Aberrant Polycystin-1 Expression Results in Modification of Activator Protein-1 Activity, whereas Wnt Signaling Remains Unaffected. J Biol Chem. 279: 27472-27481 (2004)

182) Clevers, H. Wnt signaling: Ig-norrin the dogma. Curr Biol. 14: R436-437 (2004) 183) Baas, A.F., Smit, L., Clevers H. LKB1 tumor suppressor protein: PARtaker in cell polarity.

Trends Cell Biol. 14: 312-319 (2004) 184) Sansom, O.J., Reed, K.R., Hayes, A.J., Ireland, H., Brinkmann, H., Newton, I.P., Batlle, E.,

Simon-Assmann, P., Clevers, H., Nathke, I.S., Clarke, A.R., Winton, D.J. Loss of Apc in vivo immediately perturbs Wnt signaling, differentiation, and migration. Genes Dev. 18: 1385-1390 (2004)

185) Blache, P., van de Wetering, M., Duluc, I., Domon, C., Berta, P., Freund, J.N., Clevers, H., Jay, P. SOX9 is an intestine crypt transcription factor, is regulated by the Wnt pathway, and represses the CDX2 and MUC2 genes. J Cell Biol. 166: 37-47 (2004)

186) Boersma-Vreugdenhil, G.R., Kuipers, J., van Stralen, E., Peeters,T., Michaux, L., Hagemeijer, A., Pearson, P.L., Clevers, H.C., Bast, B.J. The recurrent translocation t(14;20)(q32;q12) in multiple myeloma results in aberrant expression of MAFB: a molecular and genetic analysis of the chromosomal breakpoint. Br J Haematol. 26: 355-363 (2004)

187) Clevers H. Signaling mucins in the (S)limelight. Dev Cell 7: 150-151 (2004)

188) Sancho E., Batlle E., Clevers H. Signaling Pathways in Intestinal Development and Cancer. Annu Rev Cell Dev Biol. 20: 695-723 (2004)

189) Clevers, H. At the crossroads of inflammation and cancer. Review. Cell. 118: 671-674 (2004) 190) Staal, F.J., Clevers, H.C. WNT signalling and haematopoiesis: a WNT-WNT situation.

Nat Rev Immunol. 5: 21-30 (2005) 191) Laoukili, J., Kooistra, M.R., Bras, A., Kauw, J., Kerkhoven, R.M., Morrison, A., Clevers, H.,

Medema, R.H. FoxM1 is required for execution of the mitotic programme and chromosome stability. Nat Cell Biol. 7: 126-136 (2005)

192) Pinto, D and Clevers, H. Wnt, stem cells and cancer in the intestine. Review. Biol Cell. 97: 185-196 (2005)

193) Forcet, C., Etienne-Manneville, S., Gaude, H., Fournier, L., Debilly, S., Salmi M., Baas A., Olschwang S., Clevers H., Billaud, M. Functional analysis of Peutz-Jeghers mutations reveals that the LKB1 carboxy-terminal region exerts a crucial role in regulating both the AMPK pathway and cell polarity. Hum Mol Genet. 14: 1283-1292 (2005)

194) Radtke, F and Clevers, H. Self-renewal and cancer of the gut: Two sides of a coin. Review. Science 307: 1904-1909 (2005)

195) Van Es, J.H., Jay, P., Gregorieff, A., van Gijn, M.E., Jonkheer, S., Hatzis, P., Thiele, A., Van den Born, M., Begthel, H., Brabletz, T., Taketo, M.M., Clevers, H. Wnt signalling induces maturation of Paneth cells in intestinal crypts. Nat Cell Biol. 7: 381-386 (2005)

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196) Sansom, O.J., Reed, K.R., van de Wetering, M., Muncan, V., Winton, D.J., Clevers, H., Clarke, A.R. Cyclin D1 is not an immediate target of beta-catenin following Apc loss in the intestine. J Biol Chem. 280: 28463-28467 (2005)

197) Riedl, J.A., Brandt, D.T., Batlle, E., Price, L.S., Clevers, H., Bos, J.L. Downregulation of Rap1 activity is involved in ephrinB1-induced cell contraction. Biochem J. 389: 465-469 (2005)

198) Reya, T., Clevers, H. Wnt signalling in stem cells and cancer. Review . Nature 434: 843-850 (2005)

199) Gregorieff, A., Clevers, H. Wnt signaling in the intestinal epithelium: from endoderm to cancer. Review. Genes Dev. 19: 877-890 (2005)

200) Glass, D.A. 2nd, Bialek, P., Ahn, J.D., Starbuck, M., Patel, M.S., Clevers, H., Taketo M.M., Long F., McMahon A.P., Lang R.A., Karsenty G. Canonical Wnt signaling in differentiated osteoblasts controls osteoclast differentiation. Dev Cell. 8: 751-764 (2005)

201) Pinto, D. Clevers, H. Wnt control of stem cells and differentiation in the intestinal epithelium. Exp Cell Res. 306: 357-363 (2005)

202) Van Es, J.H., Van Gijn, M.E., Riccio, O., Van den Born, M., Vooijs, M., Begthel, H., Cozijnsen, M., Robine, S., Winton, D.J., Radtke, F., Clevers, H. Notch pathway/γ-secretase inhibition turns proliferative cells in intestinal crypts and neoplasia into Goblet cells. Nature 435: 959-963 (2005)

203) Batlle, E., Bacani, J., Begthel, H., Jonkheer, S., Gregorieff, A., Van de Born, M., Malats, N., Sancho, E., Boon, E., Pawson, T., Gallinger, S., Pals, S., Clevers, H. EphB activity suppresses colorectal cancer progression. Nature 435: 1126-1130 (2005)

204) Gregorieff, A., Pinto, D., Begthel, H., Destree, O., Kielman, M., Clevers, H. Expression pattern of Wnt signaling components in the adult intestine. Gastroenterology 129: 626-638 (2005)

205) Clevers, J.C. Improving the health of scientific research in The Netherlands. Ned Tijdschr Geneeskd. 149: 1608-1611 (2005)

206) Clevers, H. Stem cells, asymmetric division and cancer. Nature Genetics 37: 1027-1028 (2005) 207) Van Es, J.H., Clevers, H. Notch and Wnt inhibitors as potential new drugs for intestinal neoplastic

disease. Trends Mol Med. 11: 496-502 (2005)

208) Wilson, M.E., Yang, K.Y., Kalousova, A., Lau, J., Kosaka, Y., Lynn, F.C., Wang, J., Mrejen, C., Episkopou, V., Clevers, H.C., German, M.S. The HMG Box Transcription Factor Sox4 Contributes to the Development of the Endocrine Pancreas. Diabetes 54: 3402-3409 (2005)

209) Malliri, A., Rygiel, T.P., van der Kammen, R.A., Song, J.Y., Engers, R., Hurlstone, A.F., Clevers, H., Collard, J.G. The RAC activator Tiam1 is a Wnt-responsive gene that modifies intestinal tumour development. J Biol Chem. 6: 543-548 (2006)

210) Jansen, M, de Leng, W.W., Baas, A.F., Myoshi, H., Mathus-Vliegen, L., Taketo, M.M., Clevers, H., Giardiello, F.M., Offerhaus, G.J. Mucosal prolapse in the pathogenesis of Peutz-Jeghers polyposis. Gut 55: 1-5 (2006)

211) Clevers, H, Batlle, E. EphB/EphrinB receptors and Wnt signaling in colorectal cancer. Cancer Res. 66: 2-5 (2006)

212) Giles, R.H., Lolkema, M.P., Snijckers, C.M., Belderbos, M., van der Groep, P., Mans, D.A., van Beest, M., van Noort, M., Goldschmeding, R., van Diest, P.J., Clevers, H., Voest, E.E. Interplay between VHL/HIF1alpha and Wnt/beta-catenin pathways during colorectal tumorigenesis. Oncogene 25: 3065-3070 (2006)

213) Holstege, F.C., Clevers, H. Transcription factor target practice. Cell 124: 21-23 (2006)

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214) Haramis, A.P., Hurlstone, A., Van der Velden, Y., Begthel, H., Van den Born, M., Offerhaus, G.J., Clevers, H.C. Adenomatous polyposis coli-deficient zebrafish are susceptible to digestive tract neoplasia. EMBO Rep. 7: 444-449 (2006)

215) Clevers, H. Colon cancer--understanding how NSAIDs work. N Engl J Med. 354: 761-763 (2006)

216) Huang, Z., Xie, H., Ioannidis, V., Held, W., Clevers, H., Sadim, M.S., Sun, Z. Transcriptional regulation of CD4 gene expression by T cell factor-1/beta-catenin pathway. J Immunol. 176: 4880-4887 (2006)

217) Thiele, A., Nagamine, Y., Hauschildt, S., Clevers, H. AU-rich elements and alternative splicing in the beta-catenin 3'UTR can influence the human beta-catenin mRNA stability. Exp Cell Res. 312: 2367-2378 (2006)

218) Radtke F., Clevers H. Riccio O. From gut homeostasis to cancer. Curr Mol Med. 6: 275-289 (2006)

219) Markiewicz, E., Tilgner, K., Barker, N., van de Wetering, M., Clevers, H., Dorobek, M., Hausmanowa-Petrusewicz, I., Ramaekers, F.C., Broers, J.L., Blankesteijn, W.M., Salpingidou, G., Wilson, R.G., Ellis, J.A., Hutchison, C.J. The inner nuclear membrane protein Emerin regulates beta-catenin activity by restricting its accumulation in the nucleus. EMBO J. 25: 3275-3285 (2006)

220) Muncan, V., Sansom, O.J., Tertoolen, L., Phesse, T.J., Begthel, H., Sancho, E., Cole, A.M., Gregorieff, A., de Alboran, I.M., Clevers, H., Clarke, A.R. Rapid loss of intestinal crypts upon conditional deletion of the Wnt/Tcf-4 target gene c-Myc. Mol Cell Biol. 26: 8418-8426 (2006)

221) Clevers, H. Wnt/β-catenin signaling in development and disease. Review. Cell 127: 469-480 (2006)

222) Barker, N, Clevers, H. Mining the Wnt pathway for cancer therapeutics. Nat Rev Drug Discov. 5: 997-1014 (2006)

223) de Lau, W., Barker, N., Clevers, H. WNT signaling in the normal intestine and colorectal cancer. Review. Front Biosci. 12: 471-491 (2007)

224) Carretero, J., Medina, P.P., Blanco, R., Smit, L., Tang, M., Roncador, G., Maestre, L., Conde, E., Lopez-Rios, F., Clevers, H.C., Sanchez-Cespedes, M. Dysfunctional AMPK activity, signalling through mTOR and survival in response to energetic stress in LKB1-deficient lung cancer. Oncogene 52: 31-58 (2007)

225) Vooijs, M., Ong, C.T., Hadland, B., Huppert, S., Liu, Z., Korving, J., van den Born, M., Stappenbeck, T., Wu, Y., Clevers, H., Kopan, R. Mapping the consequence of Notch1 proteolysis in vivo with NIP-CRE. Development 134 :535-544 (2007)

226) Van der Flier, L.G., Sabates-Bellver, J., Oving, I., Haegebarth, A., De Palo, M., Anti, M., Van Gijn, M.E., Suijkerbuijk, S., Van de Wetering, M., Marra, G., Clevers, H. The Intestinal Wnt/TCF Signature. Gastroenterology 132: 628-632 (2007)

227) Malaterre, J., Carpinelli, M., Ernst, M., Alexander, W., Cooke, M., Sutton, S., Dworkin, S., Heath, J.K., Frampton, J., McArthur, G., Clevers, H., Hilton, D., Mantamadiotis, T., Ramsay, R.G. c-Myb is required for progenitor cell homeostasis in colonic crypts. Proc Natl Acad Sci U S A 104: 3829-3834 (2007)

228) Sansom, O.J., Meniel, V.S., Muncan, V., Phesse, T.J., Wilkins, J.A., Reed, K.R., Vass, J.K., Athineos, D., Clevers, H., Clarke, A.R. Myc deletion rescues Apc deficiency in the small intestine. Nature 446: 676-679 (2007)

229) Gregorieff, A., Clever,s H. APCmin tumors need a SPARC. Gut 56: 1341-1343 (2007) 230) Kojima, Y., Miyoshi, H., Clevers, H.C., Oshima, M., Aoki, M., Taketo, M.M. Suppression of tubulin

polymerisation by the LKB1-MARK signaling. J Biol Chem. 282: 23532-23540 (2007)

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231) Mori-Akiyama, Y., van den Born, M., van Es, J.H., Hamilton, S.R., Adams, H.P., Zhang, J., Clevers, H., de Crombrugghe B. SOX9 is required for the differentiation of paneth cells in the intestinal epithelium. Gastroenterology 133:539-546 (2007)

232) Brugmann, S.A., Goodnough, L.H., Gregorieff, A., Leucht, P., Ten Berge, D., Fuerer, C., Clevers, H., Nusse, R., Helms, J.A. Wnt signaling mediates regional specification in the vertebrate face. Development 134: 3283-3295 (2007)

233) Wehkamp, J., Wang, G., Kubler, I., Nuding, S., Gregorieff, A., Schnabel, A., Kays, R.J., Fellermann, K., Burk, O., Schwab, M., Clevers, H., Bevins, C.L., Stange, E.F. The Paneth Cell {alpha}-Defensin Deficiency of Ileal Crohn's Disease Is Linked to Wnt/Tcf-4. J Immunol. 179: 3109-3118 (2007)

234) Muncan, V., Faro, A., Haramis, A.P., Hurlstone, A.F., Wienholds, E., van Es, J., Korving, J., Begthel, H., Zivkovic, D., Clevers, H. T-cell factor 4 (Tcf7l2) maintains proliferative compartments in zebrafish intestine. EMBO Rep. 8: 966-973 (2007)

235) de Leng, W.W., Jansen, M., Keller, J.J., de Gijsel, M., Milne, A.N., Morsink, F.H., Weterman, M.A., Iacobuzio-Donahue, C.A., Clevers, H.C., Giardiello, F.M., Offerhaus, G.J. Peutz Jeghers syndrome polyps are polyclonal with expanded progenitor cell compartment. Gut 56: 1475-1476 (2007)

236) Barker, N., Van Es, J.H., Kuipers, J., Kujala, P., Van den Born, M., Cozijnsen, M., Haegebarth, A., Korving, J., Begthel, H., Peters, P.J., Clevers, H. Identification of stem cells in small intestine and colon by the marker gene LGR5. Nature 449: 1003-1007 (2007)

237) Kessler, B.M., Fortunati, E., Melis, M., Pals, C.E., Clevers, H., Maurice, M.M. Proteome Changes Induced by Knock-Down of the Deubiquitylating Enzyme HAUSP/USP7. J Proteome Res. 6: 4163-4172 (2007)

238) van Noort, M., Weerkamp, F., Clevers, H.C., Staal, F.J. Wnt signaling and phosphorylation status of beta-catenin: importance of the correct antibody tools. Blood 110: 2778-2779 (2007)

239) Barker, N. and Clevers, H. Tracking Down the Stem Cells of the Intestine: Strategies to Identify Adult Stem Cells. Gastroenterology 133: 1755-1760 (2007)

240) Sabates-Bellver, J., Van der Flier, L.G., de Palo, M., Cattaneo, E., Maake, C., Rehrauer, H., Laczko, E., Kurowski, M.A., Bujnicki, J.M., Menigatti, M., Luz, J., Ranalli, T.V., Gomes, V., Pastorelli, A., Faggiani, R., Anti, M., Jiricny, J., Clevers, H., Marra, G. Transcriptome profile of human colorectal adenomas. Mol Cancer Res. 5: 1263-1275 (2007)

241) Hatzis, P., van der Flier, L.G., van Driel, M.A., Guryev, V., Nielsen, F., Denissov, S., Nijman, I.J., Koster, J., Santo, E.E., Welboren, W., Versteeg, R., Cuppen, E., van de Wetering M., Clevers H., Stunnenberg H.G. Genome-wide pattern of TCF7L2/TCF4 chromatin occupancy in colorectal cancer cells. Mol Cell Biol. 28: 2732-2744 (2008)

242) Riccio, O., van Gijn, M.E., Bezdek, A.C., Pellegrinet, L., van Es, J.H., Zimber-Strobl, U., Strobl, L.J., Honjo, T., Clevers, H., Radtke, F. Loss of intestinal crypt progenitor cells owing to inactivation of both Notch1 and Notch2 is accompanied by derepression of CDK inhibitors p27Kip1 and p57Kip2. EMBO Rep. 9: 377-383 (2008)

243) Burns, C.J., Zhang, J., Brown, E.C., Van Bibber, A.M., Van Es, J., Clevers, H., Ishikawa, T.O., Taketo, M.M., Vetter, M.L., Fuhrmann, S. Investigation of Frizzled-5 during embryonic neural development in mouse. Dev Dyn. 237: 1614-1626 (2008)

244) Goessling, W., North, T.E., Lord, A.M., Ceol, C., Lee, S., Weidinger, G., Bourque, C., Strijbosch, R., Haramis, A.P., Puder, M., Clevers, H., Moon, R.T., Zon, L.I. APC mutant zebrafish uncover a

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changing temporal requirement for wnt signaling in liver development. Dev Biol. 320: 161-174 (2008)

245) Barker, N., van de Wetering, M., Clevers, H. The intestinal stem cell. Review. Genes Dev. 22: 1856-1864 (2008)

246) Lips, D.J., Barker, N., Clevers, H., Hennipman A. The role of APC and beta-catenin in the etiology of aggressive fibromatosis (desmoid tumors). Eur J Surg Oncol 35: 3-10 (2008)

247) van der Flier, L.G., Clevers, H. Stem Cells, Self-Renewal, and Differentiation in the Intestinal Epithelium. Review. Annu Rev Physiol 71: 241-260 (2008)

248) Stralen, E., Leguit, R.J., Begthel, H., Michaux, L., Buijs, A., Lemmens, H., Scheiff, J.M., Doyen, C., Pierre, P., Forget, F., Clevers, H.C., Bast, B. MafB oncoprotein detected by immunohistochemistry as a highly sensitive and specific marker for the prognostic unfavorable t(14;20) (q32;q12) in multiple myeloma patients. Leukemia 23: 801-803 (2008)

249) Jaks, V., Barker, N, Kasper, M., van Es, J.H., Snippert, H.J., Clevers, H., Toftgård, R. Lgr5 marks cycling, yet long-lived, hair follicle stem cells. Nat Genet. 40: 1291-1299 (2008)

250) Barker, N., van Es, J.H., Jaks, V., Kasper, M., Snippert, H., Toftgård, R., Clevers, H. Very Long-term Self-renewal of Small Intestine, Colon, and Hair Follicles from Cycling Lgr5+ve Stem Cells. Cold Spring Harb Symp Quant Biol. 73: 351-356 (2008)

251) Offerhaus, G.J., Milne, A.N., Oving, I.M., van Gijn, M.E., Hruban, R.H., Clevers, H. A transgenic mouse model for "lipid hang-up", or why pathologists need to be involved in genetically engineered mouse modelling. Gut 57: 1239-1240 (2008)

252) van Stralen, E., van de Wetering, M., Agnelli, L., Neri, A., Clevers, H.C., Bast, B.J. Identification of primary MAFB target genes in multiple myeloma. Exp Hematol. 37: 78-86 (2009)

253) Barker, N., Ridgway, R.A., van Es, J.H.,van de Wetering, M., Begthel, H., van den Born, M., Danenberg, E., Clarke, A.R., Sansom, O.J., Clevers, H. Crypt Stem Cells as the Cells-of-Origin of Intestinal Cancer. Nature 457: 608-611 (2009)

254) Medina, P.P., Castillo, S.D., Blanco, S., Sanz-Garcia, M., Largo, C., Alvarez, S., Yokota, J., Gonzalez-Neira, A., Benitez, J., Clevers, H.C., Cigudosa, J.C., Lazo, P.A., Sanchez-Cespedes, M. The sry-hmg box gene, sox4, is a target of gene amplification at chromosome 6p in lung cancer. Hum Mol Genet. 18: 1343-52 (2009)

255) Haegebarth, A., Clevers, H. Wnt Signaling, Lgr5, and Stem Cells in the Intestine and Skin. Am J Pathol. 174: 7150721 (2009)

256) Clevers, H. Inflammatory bowel disease, stress, and the endoplasmic reticulum. N Engl J Med. 12;360: 726-727 (2009)

257) van der Flier, L.G., van Gijn, M.E., Hatzis, P., Kujala, P., Haegebarth, A., Stange, D.E., Begthel, H., van den Born, M., Guryev, V., Oving, I., van Es, J.H., Barker, N.Peters, P.J., van de Wetering, M. and Clevers, H. Transcription Factor Achaete Scute-Like 2 Controls Intestinal Stem Cell Fate. Cell 136: 903-912 (2009)

258) Snippert, H.J., van Es, J.H., van den Born, M., Begthel, H., Stange, D.E., Barker, N., Clevers, H. Prominin-1/CD133 marks stem cells and early progenitors in mouse small intestine. Gastroenterology 136: 2051-2054 (2009)

259) Sato, T., Vries, R., Snippert, H., van de Wetering, M., Barker, N., Stange, D., van Es, J., Abo, A., Kujala, P., Peters, P., and Clevers, H. Single lgr5 gut stem cells build crypt-villus structures in vitro without a stromal niche. Nature 459 :262-265 (2009)

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260) Faro, A, Boj, S.F., Ambrósio, R., van den Broek, O., Korving, J., Clevers, H. T-cell factor 4 (tcf7l2) is the main effector of Wnt signaling during zebrafish intestine organogenesis. Zebrafish 6: 59-68 (2009)

261) ten Klooster, J.P., Jansen, M., Yuan, J., Oorschot, V., Begthel, H., Di Giacomo, V., Colland, F., de Koning, J., Maurice, M.M., Hornbeck, P., Clevers H. Mst4 and Ezrin induce brush borders downstream of the Lkb1/Strad/Mo25 polarization complex. Dev Cell. 16: 551-562 (2009)

262) van der Flier, L.G., Haegebarth, A., Stange, D.E., van de Wetering, M., Clevers H. OLFM4 Is a Robust Marker for Stem Cells in Human Intestine and Marks a Subset of Colorectal Cancer Cells. Gastroenterology 137: 15-17 (2009)

263) Ye, F., Chen, Y., Hoang, T., Montgomery, R.L., Zhao, X.H., Bu, H., Hu, T., Taketo, M.M., van Es, J.H., Clevers, H., Hsieh, J., Bassel-Duby, R., Olson, E.N., Lu, Q.R. HDAC1 and HDAC2 regulate oligodendrocyte differentiation by disrupting the beta-catenin-TCF interaction. Nat Neurosci 12: 829-838 (2009)

264) Gregorieff, A., Stange, D.E., Kujala, P., Begthel, H., van den Born, M., Korving, J., Peters P.J., Clevers, H. The Ets-Domain Transcription Factor Spdef Promotes Maturation of Goblet and Paneth Cells in the Intestinal Epithelium. Gastroenterology 137: 1333-1345.e1-3 (2009)

265) Jansen, M., Ten Klooster, J.P., Offerhaus, G.J., Clevers, H. LKB1 and AMPK Family Signaling: The Intimate Link Between Cell Polarity and Energy Metabolism. Physiol Rev. 89: 777-798 (2009)

266) Hollestelle, A., Nagel, J.H., Smid, M., Lam, S., Elstrodt, F., Wasielewski, M., Ng, S.S., French, P.J., Peeters, J.K., Rozendaal, M.J., Riaz, M., Koopman, D.G., Ten Hagen, T.L., de Leeuw, B.H., Zwarthoff, E.C., Teunisse, A., van der Spek, P.J., Klijn, J.G., Dinjens, W.N., Ethier, S.P., Clevers, H., Jochemsen, A.G., den Bakker, M.A., Foekens, J.A., Martens, J.W., Schutte, M. Distinct gene mutation profiles among luminal-type and basal-type breast cancer cell lines. Breast Cancer Res Treat. 121: 53-64 (2009)

267) Begus-Nahrmann, Y., Lechel, A., Obenauf, A.C., Nalapareddy, K., Peit, E., Hoffmann, E., Schlaudraff, F., Liss, B., Schirmacher, P., Kestler, H., Danenberg, E., Barker, N., Clevers, H., Speicher, M.R., Rudolph, K.L. p53 deletion impairs clearance of chromosomal-instable stem cells in aging telomere-dysfunctional mice. Nat Genet. 41: 1138-1143 (2009)

268) Fu, H., Cai, J., Clevers, H., Fast, E., Gray, S., Greenberg, R., Jain, M.K., Ma, Q., Qiu, M., Rowitch, D.H., Taylor, C.M., Stiles, C.D. A genome-wide screen for spatially restricted expression patterns identifies transcription factors that regulate glial development. J Neurosci. 29: 11399-11408 (2009)

269) Chen, G., Korfhagen. T.R., Xu, Y., Kitzmiller, J., Wert, S.E., Maeda, Y., Gregorieff, A., Clevers, H., Whitsett, J.A. SPDEF is required for mouse pulmonary goblet cell differentiation and regulates a network of genes associated with mucus production. J Clin Invest. 119: 2914-2924 (2009)

270) Mahmoudi, T., Li, V.S., Ng, S.S., Taouatas, N,. Vries, R.G., Mohammed, S., Heck, A.J., Clevers, H. The kinase TNIK is an essential activator of Wnt target genes. EMBO J. 28: 3329-3340 (2009)

271) Clevers, H. Eyeing up new Wnt pathway players. Review. Cell 139: 227-229 (2009) 272) Ng, S.S., Mahmoudi, T., Danenberg, E., Bejaoui, I., de Lau, W., Korswagen, H.C., Schutte, M.,

Clevers, H. Phosphatidylinositol 3-kinase (PI3K) signaling does not activate the Wnt cascade. J Biol Chem. 284: 35308-35313 (2009)

273) Faro, A, Boj, S.f., Clevers, H. Fishing for Intestinal Cancer Models: Unraveling Gastrointestinal Homeostasis and Tumorigenesis in Zebrafish. Zebrafish 6: 361-376 (2009)

274) Clevers, H. Searching for adult stem cells in the intestine. EMBO Mol Med. 1: 255-259 (2009)

275) Gregorieff, A, Clevers, H. In situ hybridization to identify gut stem cells. Curr Protoc Stem Cell Biol. Chapter 2: Unit 2F.1 (2010)

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276) Menke, V., van Es, J.H., de Lau, W., van den Born, M., Kuipers, E.J., Siersema, P.D., de Bruin, R.W., Kusters, J.G., Clevers, H. Conversion of metaplastic Barrett's epithelium into post-mitotic goblet cells by gamma-secretase inhibition. Dis Model Mech. 3: 104-110 (2010)

277) Barker, N., Huch, M., Kujala, P., van de Wetering, M., Snippert, H.J., van Es, J.H., Sato, T., Stange, D.E., Begthel, H., van den Born, M., Danenberg, E., van den Brink, S., Korving, J., Abo, A., Peters, P.J., Wright, N., Poulsom, R., Clevers, H. Lgr5(+ve) stem cells drive self-renewal in the stomach and build long-lived gastric units in vitro. Cell Stem Cell 6: 25-36 (2010)

278) Li, L. and Clevers, H. Coexistence of Quiescent and Active Adult Stem Cells in Mammals. Review Science 327: 542-545 (2010)

279) Ng, S.S., Mahmoudi, T., Li, V.S., Hatzis, P., Boersema, P.J., Mohammed, S., Heck, A.J., Clevers, H. MAP3K1 functionally interacts with Axin1 in the canonical Wnt signalling pathway. Biol Chem. 391: 171-80 (2010)

280) Quyn, A.J., Appleton, P.L.., Carey, F.A, Steele, R.J., Barker, N., Clevers, H., Ridgway, R.A., Sansom, O.J., Näthke, I.S. Spindle orientation bias in gut epithelial stem cell compartments is lost in precancerous tissue. Cell Stem Cell 6: 175-181 (2010)

281) Snippert, H.J., Haegebarth, A., Kasper, M., Jaks, V., van Es, J.H., Barker, N., van de Wetering, M., van den Born, M., Begthel, H., Vries, R.G., Stange, D.E., Toftgård, R., Clevers H. Lgr6 marks stem cells in the hair follicle that generate all cell lineages of the skin. Science 327: 1385-1389 (2010)

282) Tauriello, D.V., Haegebarth, A., Kuper, I., Edelmannm M.J., Henraat, M., Canninga-van Dijk, M.R., Kessler, B.M., Clevers, H., Maurice, M.M. Loss of the tumor suppressor CYLD enhances Wnt/beta-catenin signaling through K63-linked ubiquitination of Dvl. Mol Cell. 37: 607-619 (2010)

283) Oerlemans, M.I., Goumans, M.J., van Middelaar, B., Clevers, H., Doevendans, P.A., Sluijter, J.P. Active Wnt signaling in response to cardiac injury. Basic Res Cardiol. 105: 6316-6341 (2010)

284) Barker, N., Clevers, H. Leucine-rich repeat-containing G-protein-coupled receptors as markers of adult stem cells. Review Gastroenterology 138: 1681-1696 (2010)

285) Barker, N., Clevers, H. Lineage tracing in the intestinal epithelium. Curr Protoc Stem Cell Biol. Chapter 5:Unit5A.4 (2010)

286) van Es, J.H., de Geest, N., van de Born, M., Clevers, H., Hassan, B.A. Intestinal stem cells lacking the Math1 tumor suppressor are refractory to Notch inhibitors. Nature Communication 1:1-5. (2010)

287) Kosinski, C., Stange, D.E., Xu, C., Chan, A.S., Ho, C., Yuen, S.T., Mifflin, R.C., Powell, D.W., Clevers, H., Leung, S.Y., Chen, X. Indian Hedgehog Regulates Intestinal Stem Cell Fate through Epithelial-Mesenchymal Interactions During Development. Gastroenterology 139: 893-903 (2010)

288) Verheyen, E.M., Clevers, H. Wnts as self-renewal factors: mammary stem cells and beyond. Cell Stem Cell. 6: 494-495 (2010)

289) Vries, R.G., Huch, M, Clevers, H. Stem cells and cancer of the stomach and intestine. Mol Oncol. 4: 373-384 (2010)

290) Verzi, M.P., Hatzis, P., Sulahian, R., Philips, J., Schuijers, J., Shin, H., Freed, E., Lynch, J.P., Dang, D.T., Brown, M., Clevers, H., Liu, X.S., Shivdasani, R.A. TCF4 and CDX2, major transcription factors for intestinal function, converge on the same cis-regulatory regions. Proc Natl Acad Sci U S A. 107:15157-15162 (2010)

291) Ashton, G.H., Mortonm J.P., Myant,K.,Phesse, T.J., Ridgway, R.A., Marsh, V., Wilkins, J.A., Athineos, D., Muncan, V., Kemp, R., Neufeld, K., Clevers, H., Brunton, V., Winton, D.J., Wang, X., Sears, R.C., Clarke, A.R., Frame, M.C., Sansom, O.J. Focal adhesion kinase is required for

http://www.ncbi.nlm.nih.gov/pubmed/20708588�

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intestinal regeneration and tumorigenesis downstream of Wnt/c-Myc signaling. Dev Cell 19: 259-269 (2010)

292) Cole, A.M., Myant, K., Reed, K.R., Athineos, D., Ridgway, R.A., Muncan, V., Clevers, H., Clarke, A.R., Sicinski, P., Sansom, O.J. Cyclin D2-CDK4/6 is required for efficient proliferation and tumorigenesis following Apc loss. Cancer Res. 70: 8149-58 (2010)

293) Snippert, .J., van der Flier, L.G., Sato, T., van Es, J.H., van den Born, M., Kroon-Veenboer, C., Barker, N.,Klein, A.M., van Rheenen, J. Benjamin D. Simons, B.D. and Clevers, H. Intestinal Crypt Homeostasis results from Neutral Competition between Symmetrically Dividing Lgr5 Stem Cells. Cell 143:134-44 (2010)

294) Demarque, M.D., Nacerddine, K., Neyret-Kahn, H., Andrieux, A., Danenberg, E., Jouvion, G., Bomme, P., Hamard, G., Romagnolo, B., Terris, B., Cumano, A., Barker, N., Clevers, H., Dejean, A. Sumoylation by Ubc9 Regulates the Stem Cell Compartment and Structure and Function of the Intestinal Epithelium in Mice. Gastroenterology 140: 286-296 (2010)

295) van Es, J.H., de Geest, N., van de Born, M., Clevers, H., Hassan, B.A. Intestinal stem cells lacking the Math1 tumour suppressor are refractory to Notch inhibitors. Nat Commun. 1: 1-5 (2010)

296) Cole, A.M., Ridgway, R.A., Derkits, S.E., Parry, L., Barker, N., Clevers, H., Clarke, A.R., Sansom, O.J. p21 loss blocks senescence following Apc loss and provokes tumourigenesis in the renal but not the intestinal epithelium. EMBO Mol Med. 2: 472-486 (2010)

297) Bongers, G., Maussang, D., Muniz, L.R., Noriega, V.M., Fraile-Ramos, A., Barker, N., Marchesi, F., Thirunarayanan, N., Vischer, H.F., Qin, L., Mayer, L., Harpaz, N., Leurs, R., Furtado, G.C., Clevers, H., Tortorella, D., Smit, M.J., Lira, S.A. The cytomegalovirus-encoded chemokine receptor US28 promotes intestinal neoplasia in transgenic mice. J Clin Invest 120: 3969-3978 (2010)

298) Qiu, W., Wang, X., Leibowitz, B., Liu, H., Barker, N., Okada, H., Oue, N., Yasui, W., Clevers, H., Schoen, R.E., Yu, J., Zhang, L. Chemoprevention by nonsteroidal anti-inflammatory drugs eliminates oncogenic intestinal stem cells via SMAC-dependent apoptosis. Proc Natl Acad Sci U S A (2010)

299) Mahmoudi, T., Boj, S.F., Hatzis, P., Li, V.S., Taouatas, N., Vries, R.G., Teunissen, H., Begthel, H., Korving, J., Mohammed,S., Heck, A.J., Clevers, H. The Leukemia-Associated Mllt10/Af10-Dot1l Are Tcf4/β-Catenin Coactivators Essential for Intestinal Homeostasis. PLoS Biol.8: e1000539 (2010)

300) Barker, N., Bartfeld, S., Clevers, H. Tissue-resident adult stem cell populations of rapidly self-renewing organs. Cell Stem Cell. 7: 656-670 (2010)

301) Sato, T., van Es, J.H., Snippert, H.J., Stange, D.E., Vries, R.G., van den Born, M., Barker, N., Shroyer, N.F., van de Wetering, M., Clevers, H. Paneth cells constitute the niche for Lgr5 stem cells in intestinal crypts. Nature 469: 415-418 (2011)

302) Mokry, M., Hatzis, P., de Bruijn, E., Koster, J., Versteeg, R., Schuijers, J., van de Wetering, M., Guryev, V., Clevers, H., Cuppen, E. Efficient double fragmentation ChIP-seq provides nucleotide resolution protein-DNA binding profiles. PLoS One.5: e15092 (2010)

303) Huch, M., Clevers, H. Sox9 marks adult organ progenitors. Nat Genet. 43: 9-10 (2011)

304) Snippert, H.J., Clevers, H. Tracking adult stem cells EMBO Rep. 12:113-122 (2011)





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305) Buske, P., Galle, J., Barker, N., Aust, G., Clevers, H., Loeffler, M. A comprehensive model of the spatio-temporal stem cell and tissue organisation in the intestinal crypt. PLoS Comput Biol. 7(1): e1001045 (2011)

306) Schepers, A.G., Vries, R., van den Born, M., van de Wetering, M., Clevers, H. Lgr5 intestinal stem cells have high telomerase activity and randomly segregate their chromosomes EMBO J. 30: 1104-1109 (2011)

307) Gerbe, F., van Es, J.H., Makrini, L. Brulin, B.,Mellitzer, G., Robine, S., Romagnolo, B., Shroyer, N.F., Bourgaux, J-F.,Pignodel, C., Clevers, H., Jay, P. Distinct ATOH1 and Neurog3 requirements define tuft cells as a new secretory cell type in the intestinal epithelium. JCB 192: 767-780 (2011)

308) van der Velden, Y.U., Wang, L., Zevenhoven, J., van Rooijen, E., van Lohuizen, M., Giles, R.H., Clevers, H., Haramis, A.P. The serine-threonine kinase LKB1 is essential for survival under energetic stress in zebrafish. Proc Natl Acad Sci U S A. 108: 4358-4363 (2011)

309) Clevers, H. The cancer stem cell: premises, promises and challenges. Nat Med. 17: 313-319 (2011)

310) Merlos-Suárez, A., Barriga, F.M., Jung, P., Iglesias, M., Virtudes Céspedes, M. Rossell, D., Sevillano, M., Hernando-Momblona, X., da Silva-Diz, V., Muñoz, P. Clevers, H., Sancho, Ramón, E. Mangues, Batlle, E. The intestinal stem cell signature identifies colorectal cancer stem cells and predicts disease relapse Cell Stem Cell 8: 511-524 (2011)

311) Emmink, B.L., Van Houdt, W.J., Vries, R.G., Hoogwater, F.J., Govaert, K.M., Verheem, A., Nijkamp, M.W., Steller, E.J., Jimenez CR, Clevers H, Rinkes IH, Kranenburg O. Differentiated Human Colorectal Cancer Cells Protect Tumor-Initiating Cells from Irinotecan. Gastroenterology 141: 269-278 (2011)

312) Simons, B.D., Clevers, H. Strategies for homeostatic stem cell self-renewal in adult tissues. Cell 145: 851-862 (2011)

313) Samuel, M.S., Lopez, J.I., McGhee, E.J., Croft, D.R., Strachan, D., Timpson, P., Munro, J., Schröder, E., Zhou, J., Brunton, V.G., Barker, N., Clevers, H., Sansom, O.J., Anderson, K.I., Weaver, V.M., Olson, M.F. Actomyosin-Mediated Cellular Tension Drives Increased Tissue Stiffness and β-Catenin Activation to Induce Epidermal Hyperplasia and Tumor Growth. Cancer Cell 19: 776-791 (2011)

314) Di Palma, S., Stange, D., van de Wetering, M., Clevers, H., Heck, A.J., Mohammed, S. Highly Sensitive Proteome Analysis of FACS-Sorted Adult Colon Stem Cells. J Proteome Res. 10: 3814-3819 (2011)

315) Froeling, F.E., Feig, C., Chelala, C., Dobson, R., Mein, C.E., Tuveson, D.A., Clevers, H., Hart, I.R., Kocher, H.M. Retinoic Acid-Induced Pancreatic Stellate Cell Quiescence Reduces Paracrine Wnt-β-Catenin Signaling to Slow Tumor Progression. Gastroenterology 141: 1486-1497 (2011)

316) de Lau, W., Barker, N., Low, T.Y., Koo, B.K., Li, V.S., Teunissen, H., Kujala, P., Haegebarth, A., Peters, P.J., van de Wetering, M., Stange, D.E., van Es, J., Guardavaccaro, D., Schasfoort, R.B., Mohri, Y., Nishimori, K., Mohammed,S., Heck, A.J., Clevers, H. Lgr5 homologues associate with Wnt receptors and mediate R-spondin signalling. Nature 476: 293-297 (2011)

317) Simons, B.D., Clevers, H. Stem cell self-renewal in intestinal crypt. Exp Cell Res. 317: 2719-2724 (2011)

318) van Houdt, W.J., Emmink, B.L., Pham, T.V., Piersma, S.R., Verheem, A., Vries, R., Fratantoni, S.A., Pronk, A., Clevers, H., Borel Rinkes, I.H., Jimenez, C.R., Kranenburg, O. Comparative proteomics of colon cancer stem cells and differentiated tumor cells identifies BIRC6 as a potential therapeutic target. Mol Cell Proteomics 10: M111.011353 (2011)

319) Snippert, H.J., Schepers, A.G., Delconte, G., Siersema, P.D., Clevers, H. Slide preparation for single-cell-resolution imaging of fluorescent proteins in their three-dimensional near-native

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environment. Nat Protoc. 6: 1221-1228 (2011)

320) Sylva, M., Li, V.S., Buffing, A.A., van Es, J.H., van den Born, M., van der Velden, S., Gunst, Q., Koolstra, J.H., Moorman, A.F., Clevers, H., van den Hoff, M.J. The BMP Antagonist Follistatin-Like 1 Is Required for Skeletal and Lung Organogenesis. PLoS One. 6: e22616. (2011)

321) Sato, T., Stange, D.E., Ferrante, M., Vries, R.G., van Es, J.H., van den Brink, S., van Houdt, W.J., Pronk, A., van Gorp, J., Siersema, P.D., Clevers, H. Long-Term Expansion of Epithelial Organoids from Human Colon, Adenoma, Adenocarcinoma, and Barrett's Epithelium. Gastroenterology 141: 1762-1772 (2011)

322) Jung, P., Sato, T., Merlos-Suárez, A., Barriga, F.M., Iglesias, M., Rossell, D., Gallardo, M.M., Blasco, M.A., Sancho, E., Clevers, H., Batlle, E. Isolation and in vitro expansion of human colonic stem cells. Nat Med. 17: 1225-1227 (2011)

323) Mokry, M., Hatzis, P., Schuijers, J., Lansu, N., Ruzius, F.P., Clevers, H., Cuppen, E. Integrated genome-wide analysis of transcription factor occupancy, RNA polymerase II binding and steady-state RNA levels identify differentially regulated functional gene classes. Nucleic Acids Res. 40: 148-158 (2011)

324) Dalerba, P., Kalisky, T., Sahoo, D., Rajendran, P.S., Rothenberg, M.E., Leyrat, A.A., Sim, S., Okamoto, J., Johnston, D.M., Qian, D., Zabala, M., Bueno, J., Neff, N.F., Wang, J., Shelton, A.A., Visser, B., Hisamori, S., Shimono, Y., van de Wetering, M., Clevers, H., Clarke, M.F., Quake, S.R. Single-cell dissection of transcriptional heterogeneity in human colon tumors. Nat Biotechnol. 29: 1120-1127 (2011)

325) Itzkovitz, S., Lyubimova, A., Blat, I.C., Maynard, M., van Es, J., Lees, J., Jacks, T., Clevers, H., van Oudenaarden, A. Single-molecule transcript counting of stem-cell markers in the mouse intestine. Nat Cell Biol. 14: 106-114 (2011)

326) Nieuwenhuis, M.H., Douma, K.F., Bleiker, E.M., Aaronson, N.K., Clevers, H., Vasen, H.F. Clinical evidence for an association between familial adenomatous polyposis (FAP) and type II diabetes. Int J Cancer. 131: 1488-1489 (2012)

327) Koo, B.K., Stange, D.E., Sato, T., Karthaus, W., Farin, H.F., Huch, M., van Es, J.H., Clevers, H. Controlled gene expression in primary Lgr5 organoid cultures. Nat Methods 9: 81-83 (2011)

328) Stringer, E.J., Duluc, I., Saandi, T., Davidson, I., Bialecka, M., Sato, T., Barker, N., Clevers, H., Pritchard, C.A., Winton, D.J., Wright, N.A., Freund, J.N., Deschamps, J., Beck, F. Cdx2 determines the fate of postnatal intestinal endoderm. Development 139: 465-474 (2011)

329) Schasfoort, R.B., de Lau, W., van der Kooi, A., Clevers, H., Engbers, G.H. Method for estimating the single molecular affinity. Anal Biochem. 421: 794-796 (2012)

330) Basak, O., Clevers, H. Neural stem cells for diabetes cell-based therapy. EMBO Mol Med. 3: 698-700 (2011)

331) Itzkovitz, S., Blat, I.C., Jacks, T., Clevers, H., van Oudenaarden A. Optimality in the development of intestinal crypts. Cell. 148: 608-619 (2012)

332) Wong, V.W., Stange, D.E., Page, M.E., Buczacki, S., Wabik, A., Itami, S., van de Wetering, M., Poulsom, R., Wright, N.A., Trotter, M.W., Watt, F.M., Winton, D.J., Clevers, H., Jensen, K.B. Lrig1 controls intestinal stem-cell homeostasis by negative regulation of ErbB signalling. Nat Cell Biol. 14: 401-408 (2012)

333) van Es, J.H., Haegebarth, A., Kujala, P., Itzkovitz, S., Koo, B.K., Boj, S.F., Korving, J., van den Born, M., van Oudenaarden, A., Robine, S., Clevers, H. A critical role for the Wnt effector Tcf4 in adult intestinal homeostatic self-renewal. Mol Cell Biol. 32: 1918-1927 (2012)

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334) Lander, A.D., Kimble, J., Clevers, H., Fuchs, E., Montarras, D., Buckingham, M., Calof, A.L., Trumpp, A., Oskarsson, T. What does the concept of the stem cell niche really mean today? BMC Biol. 10:19 (2012)

335) Yui ,S., Nakamura, T., Sato, T., Nemoto, Y., Mizutani, T., Zheng, X., Ichinose, S., Nagaishi, T., Okamoto, R., Tsuchiya, K., Clevers, H., Watanabe, M. Functional engraftment of colon epithelium expanded in vitro from a single adult Lgr5(+) stem cell.

336) Nat Med. 18: 618-623 (2012) de Lau, W.B., Snel, B., Clevers, H.C. R-spondin protein family. Genome Biol. 13: 242 (2012)

337) Schepers A, Clevers H. Wnt signaling, stem cells, and cancer of the gastrointestinal tract. Cold Spring Harb Perspect Biol. 4(4):a007989 (2012)

338) Guardavaccaro, D., Clevers, H. Wnt/β-Catenin and MAPK Signaling: Allies and Enemies in Different Battlefields. Sci Signal. 5: pe15. (2012)

339) Li, V.S., Clevers, H. In Vitro Expansion and Transplantation of Intestinal Crypt Stem Cells. Gastroenterology 143: 30-34 (2012)

340) Schuijers J, Clevers H. Adult mammalian stem cells: the role of Wnt, Lgr5 and R-spondins. EMBO J. 31: 2685-2696 (2012)

341) Buske, P., Przybilla, J., Loeffler, M., Sachs, N., Sato, T., Clevers, H., Galle, J. On the biomechanics of stem cell niche formation in the gut: Modelling growing organoids. FEBS J. 279: 3475-3487 (2012)

342) Nusse, R., Clevers H. Wnt / ß catenin signaling and disease. Review Cell 149:1192-1205 (2012)

343) Li, V.S., Ng, S.S., Boersema, P.J., Low, T.Y., Karthaus, W.R., Gerlach, J.P., Mohammed, S., Heck, A.J., Maurice, M.M., Mahmoudi, T. and Clevers H. Wnt signaling inhibits proteasomal β-catenin degradation within a compositionally intact Axin1 complex. Cell 149: 1245-1256 (2012)

344) Muñoz, J., Stange, D.E., Schepers, A.G., van de Wetering, M., Koo, B-K, Itzkovitz, S., Volckmann, R., Kung, K.S., Koster, J., Radulescu, S., Myant, K., Versteeg, R., Sansom, O.J., van Es, J.H., Barker, N., van Oudenaarden, A., Mohammed, S., Heck, A.J.R., Clevers, H. The Lgr5 Intestinal Stem Cell Signature: Robust Expression of Proposed Quiescent ‘+4’ Cell Markers. EMBO J. 31: 3079-3091 (2012)

345) de Lau, W., Kujala, P., Schneeberger, K., Middendorp, S., Li, V.S., Barker, N., Martens, A., Hofhuis, F., Dekoter, R.P., Peters, P.J., Nieuwenhuis, E., Clevers, H. Peyer's Patch M cells derive from Lgr5+ stem cells require SpiB and are induced by RankL in cultured 'organoids' Mol Cell Biol. 32: 3639-3647 (2012)

346) Gloerich, M., Ten Klooster, J.P., Vliem, M.J., Koorman, T., Zwartkruis, F.J., Clevers, H., Bos, J.L. Rap2A links intestinal cell polarity to brush border formation. Nat Cell Biol. 14: 793-801 (2012)

347) Hashimoto, T., Perlot, T., Rehman, A., Trichereau, J, Ishiguro, H., Paolino, M., Sigl, V., Hanada, T., Hanada, R., Lipinski, S., Wild, B., Camargo, S.M., Singer, D., Richter, A., Kuba, K., Fukamizu, A., Schreiber, S., Clevers, H., Verrey, F., Rosenstiel, P., Penninger, J.M. ACE2 links amino acid malnutrition to microbial ecology and intestinal inflammation. Nature 487: 477-481 (2012)

348) Hua, G., Thin, T.H., Feldman, R., Haimovitz-Friedman, A., Clevers, H., Fuks, Z., Kolesnick, R. Crypt Base Columnar Stem Cells in Small Intestines of Mice are Radioresistant. Gastroenterology 143: 1266-1276 (2012)

349) Schepers, A.G., Snippert, H.J., Stange, D.E., van den Born, M., van Es, J.H., van de Wetering, M., Clevers, H. Lineage Tracing Reveals Lgr5+ Stem Cell Activity in Mouse Intestinal Adenomas. Science 337: 730-735 (2012)

350) Shaked, H., Hofseth, L.J., Chumanevich, A., Chumanevich, A.A., Wang, J., Wang, Y., Taniguchi, K., Guma, M., Shenouda, S., Clevers, H., Harris, C.C., Karin, M. Chronic epithelial NF-κB

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activation accelerates APC loss and intestinal tumor initiation through iNOS up-regulation. Proc Natl Acad Sci U S A. 109: 14007-14012

351) Koo, B-K., Spit, M. Jordens, I., Low, T.Y., Stange, D.E., van de Wetering, M., van Es, J.H., Mohammed, S., Heck, A.J.R., Maurice, M.M. and Clevers, H. Tumour suppressor RNF43 is a stem cell E3 ligase that induces endocytosis of Wnt receptors. Nature 488: 665-669 (2012)

352) Farin HF, van Es JH, Clevers H. Redundant Sources of Wnt Regulate Intestinal Stem Cells and Promote Formation of Paneth Cells. Gastroenterology 143:1518-1529 (2012)

353) Lum L, Clevers H. Cell biology. The unusual case of Porcupine. Science. 337 :922-923 (2012)

354) de Visser, K.E., Ciampricotti, M., Michalak, E., Wei-Min Tan, D., Speksnijder, E.N., Hau, C.S., Clevers, H., Barker, N., Jonkers, J. Developmental stage-specific contribution of LGR5+ cells to basal and luminal epithelial lineages in the postnatal mammary gland. J Pathol. 228: 300-309 (2012)

355) Abo, A., Clevers, H. Modulating WNT receptor turnover for tissue repair. Nat Biotechnol. 30: 835-836 (2012)

356) Kemper, K., Prasetyanti, P.R., de Lau, W., Rodermond, H., Clevers, H., Medema, J.P. Monoclonal Antibodies Against Lgr5 Identify Human Colorectal Cancer Stem Cells. Stem Cells (2012)

357) Barker, N., Rookmaaker, M.B., Kujala, P., Ng, A., Leushacke, M., Snippert, H., van de Wetering, M., Tan, S., Van Es, J.H., Huch, M., Poulsom, R., Verhaar, M.C., Peters, P.J., Clevers, H. Lgr5(+ve) Stem/Progenitor Cells Contribute to Nephron Formation during Kidney Development. Cell Rep. 2: 540-552 (2012)

358) van Es, J.H., Sato, T., van de Wetering, M., Lyubimova, A., Yee Nee, A.N., Gregorieff, A., Sasaki, N., Zeinstra, L., van den Born, M., Korving, J., Martens. A.C., Barker, N., van Oudenaarden, A., Clevers, H. Dll1(+) secretory progenitor cells revert to stem cells upon crypt damage. Nat Cell Biol. 14: 1099-1104 (2012)

359) Barker, N., van Oudenaarden, A., Clevers, H. Identifying the Stem Cell of the Intestinal Crypt: Strategies and Pitfalls Cell Stem Cell 11: 452-460 (2012)

360) Clevers, H. The Paneth cell, caloric restriction, and intestinal integrity. N Engl J Med. 367: 1560-1561 (2012)

361) Staal FJ, Clevers H. Tales of the unexpected: tcf1 functions as a tumor suppressor for leukemias. Immunity. 37: 761-763 (2012)

362) Tiemessen, M.M., Baert, M.R., Schonewille, T., Brugman, M.H., Famili, F., Salvatori, D.C., Meijerink, J.P., Ozbek, U., Clevers, H., van Dongen, J.J., Staal, F.J. The nuclear effector of wnt-signaling, tcf1, functions as a T-cell-specific tumor suppressor for development of lymphomas. PLoS Biol. 10: e1001430 (2012)

363) Wang, L., Benedito, R., Bixel, M.G., Zeuschner, D., Stehling, M., Sävendahl, L., Haigh, J.J., Snippert, H., Clevers, H., Breier, G., Kiefer, F., Adams, R.H. Identification of a clonally expanding haematopoietic compartment in bone marrow. EMBO J. 32: 219-230 (2013)

364) Huls, G., van Es, J., Clevers, H., de Haan, G., van Os, R. Loss of Tcf7 diminishes hematopoietic stem/progenitor cell function. Leukemia 27: 1613-1614 (2013)

365) Boj, S,F., van Es, J.H.,Huch. M., Li, V.S., Jose, A., Hatzis, P., Mokry, M., Haegebarth, A., van den Born, M., Chambon, P., Voshol, P., Dor, Y., Cuppen, E., Fillat, C., Clevers, H. Diabetes risk gene and Wnt effector Tcf7l2/TCF4 controls hepatic response to perinatal and adult metabolic demand. Cell 151: 1595-1607 (2012)

366) Sato, T., Clevers, H. Primary mouse small intestinal epithelial cell cultures. Methods Mol Biol. 945:319-328 (2013)

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367) Schwitalla, S., Fingerle, A.A., Cammareri, P., Nebelsiek, T., Göktuna, S.I., Ziegler, P.K., Canli, O., Heijmans, J., Huels, D.J., Moreaux, G., Rupec, R.A., Gerhard, M., Schmid, R., Barker, N., Clevers, H., Lang, R., Neumann, J., Kirchner, T., Taketo, M.M., van den Brink, G.R., Sansom, O.J., Arkan, M.C., Greten, F.R. Intestinal Tumorigenesis Initiated by Dedifferentiation and Acquisition of Stem-Cell-like Properties. Cell 152: 25-38 (2013)

368) Zeilstra, J., Joosten, S.P., van Andel, H., Tolg, C., Berns, A., Snoek, M., van de Wetering, M., Spaargaren, M., Clevers, H., Pals, S.T. Stem cell CD44v isoforms promote intestinal cancer formation in Apc(min) mice downstream of Wnt signaling. Oncogene 33: 665-670 (2014)

369) Boilève, A., Senovilla, L., Vitale, I., Lissa, D., Martins, I., Métivier, D., van den Brink, S., Clevers, H., Galluzzi, L., Castedo, M., Kroemer, .\G. Immunosurveillance against tetraploidization-induced colon tumorigenesis. Cell Cycle 12: 473-479 (2013)

370) Huch, M., Dorrell, C., Boj, S.F., van Es, J.H., van de Wetering, M., Li, V.S.W., Hamer, K., Sasaki, N., Finegold, M.J., Haft, A., Grompe, M., Clevers, H. In vitro expansion of single Lgr5+ liver stem cells induced by Wnt-driven regeneration. Nature 494: 247-250 (2013)

371) Li, V.S., Clevers, H. Intestinal Regeneration: YAP-Tumor Suppressor and Oncoprotein? Curr Biol. 23: R110-2. (2013)

372) Clevers, H. Bevins, C. Paneth Cells: Maestros of the Small Intestinal Crypts. Annu. Rev. Physiol. 75: 22.1–22.23 (2013)

373) Clevers, H. Stem Cells: A unifying theory for the crypt. Nature 495: 53-54 (2013)

374) Clevers, H., Batlle, E. SnapShot: The Intestinal Crypt. Cell 152: 1198-1198 (2013)

375) Bardehle, S., Krüger, M., Buggenthin, F., Schwausch, J., Ninkovic, J., Clevers, H., Snippert, H.J., Theis, F.J., Meyer-Luehmann, M., Bechmann, I., Dimou, L., Götz, M. Live imaging of astrocyte responses to acute injury reveals selective juxtavascular proliferation. Nat Neurosci. 16: 580-586 (2013)

376) Heijmans, J., van Lidth de Jeude, J.F., Koo, B.K., Rosekrans, S.L., Wielenga, M.C., van de Wetering, M., Ferrante, M., Lee, A.S., Onderwater, J.J., Paton, J.C., Paton, A.W., Mommaas, A.M., Kodach, L.L., Hardwick, J.C., Hommes, D.W., Clevers, H., Muncan, V., van den Brink, G.R. ER Stress Causes Rapid Loss of Intestinal Epithelial Stemness through Activation of the Unfolded Protein Response. Cell Rep. 3: 1128-1139 (2013)

377) Spijker, H.S., Ravelli, R.B., Mommaas-Kienhuis, A.M., van Apeldoorn, A.A., Engelse, M.A., Zaldumbide, A., Bonner-Weir, S., Rabelink, T.J., Hoeben, R.C., Clevers, H., Mummery, C.L., Carlotti, F., de Koning, E.J. Conversion of mature human β-cells into glucagon-producing α-cells. Diabetes 62: 2471-80 (2012)

378) Lu, J., Zhang, S., Nakano, H., Simmons, D.G., Wang,S., Kong, S., Wang, Q., Shen, L., Tu, Z., Wang, W., Wang, B., Wang, H., Wang, Y, van Es, J.H., Clevers, H., Leone, G., Cross, J.C., Wang, H. A positive feedback loop involving gcm1 and fzd5 directs chorionic branching morphogenesis in the placenta. PLoS Biol. 4: e1001536 (2013)

379) Bianco, P., Barker, R., Brüstle, O., Cattaneo, E., Clevers, H., Daley, G.Q., De Luca, M., Goldstein, L., Lindvall, O., Mummery, C., Robey, P.G., Sattler de Sousa E Brito, C., Smith, A. Regulation of stem cell therapies under attack in Europe: for whom the bell tolls. EMBO J. 32: 1489-195 (2013)

380) Marko, C.K., Menon, B.B., Chen, G., Whitsett, J.A., Clevers, H., Gipson, I.K. Spdef Null Mice Lack Conjunctival Goblet Cells and Provide a Model of Dry Eye. Am J Pathol. 183: 35-48 (2013)

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381) Kaaij, L.J., van de Wetering, M., Fang, F., Decato, B., Molaro, A., van de Werken, H.J., van Es, J.H., Schuijers, J., de Wit, E., de Laat, W., Hannon, G.J., Clevers, H.C., Smith, A.D., Ketting, R.F. DNA methylation dynamics during intestinal stem cell differentiation reveals enhancers driving gene expression in the villus. Genome Biol. 14: R50 (2013)

382) Barker N, Tan S, Clevers H. Lgr proteins in epithelial stem cell biology. Development 140: 2484-2494 (2013)

383) Dekkers, J.F., Wiegerinck, C.L., de Jonge, H.R., Bronsveld, I., Janssens, H.M., de Winter-de Groot, K.M., Brandsma, A.M., de Jong, N.W., Bijvelds, M.J., Scholte, B.J., Nieuwenhuis, E.E., van den Brink, S., Clevers, H., van der Ent, C.K., Middendorp, S., Beekman, J.M. A functional CFTR assay using primary cystic fibrosis intestinal organoids. Nat Med. 19: 939-945 (2013)

384) Sato, T., Clevers, H.. Growing self-organizing mini-guts from a single intestinal stem cell: mechanism and applications. Review Science 340 :1190-1194 (2013)

385) Peng, W.C., de Lau, W., Forneris, F., Granneman, J.C.M., Huch, M., Clevers, H. and Gros, P. structure of stem cell growth factor R-spondin 1 in complex with the ectodomain of Its receptor LGR5. Cell Reports 3: 1885-1892 (2013)

386) Huch, M., Boj, S.F., Clevers, H. Lgr5(+) liver stem cells, hepatic organoids and regenerative medicine. Regen Med. 8: 385-387 (2013)

387) Stange, D.E., Clevers, H. Concise review: the yin and yang of intestinal (cancer) stem cells and their progenitors. Stem Cells 31: 2287-2295 (2013)

388) Pilz, G.A., Shitamukai, A., Reillo, I., Pacary, E., Schwausch, J., Stahl, R., Ninkovic, J., Snippert, H.J., Clevers, H., Godinho, L., Guillemot, F., Borrell, V., Matsuzaki, F., Götz, M. Amplification of progenitors in the mammalian telencephalon includes a new radial glial cell type. Nat Commun. 4:2125 (2013)

389) Clevers, H. The intestinal crypt, a prototype stem cell compartment. Cell 154: 274-284 (2013)

390) Stange, D.E., Koo, B.K., Huch, M., Sibbel, G., Basak, O., Lyubimova, A., Kujalla, P., Bartfeld, S., Koster, J., Geahlen, J.H., Peters, P.J., van Es, J., van de Wetering, M., Mills, J.C., Clevers, H. Differentiated Troy+ chief cells act as ‘reserve’ stem cells to generate all lineages of the stomach epithelium. Cell 155: 357-368 (2013)

391) Fischer J.M., Schepers, A.G., Clevers, H., Shibata, D., Liskay, R.M. Occult progression by Apc-deficient intestinal crypts as a target for chemoprevention. Carcinogenesis 35: 237-246 (2014)

392) Sone, M., Oyama, K., Mohri, Y., Hayashi, R., Clevers, H., Nishimori, K. LGR4 expressed in uterine epithelium is necessary for uterine gland development and contributes to decidualization in mice. FASEB J. 27: 4917-4928 (2013)

393) Huch, M., Bonfanti, P., Boj, S.F., Sato, T., Loomans, C.J., van de Wetering, M., Sojoodi, M., Li, V.S., Schuijers, J., Gracanin, A., Ringnalda, F., Begthel, H., Hamer, K., Mulder, J., van Es, J.H., de Koning, E., Vries, R.G., Heimberg, H., Clevers, H. Unlimited in vitro expansion of adult bi-potent pancreas progenitors through the Lgr5/R-spondin axis. EMBO J. 32: 2708-2721(2013)

394) Clevers, H. A gutsy approach to stem cells and signalling: an interview with Hans Clevers. Dis Model Mech. 6: 1053-1056 (2013)

395) Stange, D.E., Koo, B.K., Huch, M., Sibbel, G., Basak, O., Lyubimova, A., Kujala, P., Bartfeld, S., Koster, J., Geahlen, J.H., Peters, P.J., van Es, J.H., van de Wetering, M., Mills, J.C., Clevers, H. Differentiated Troy+ chief cells act as reserve stem cells to generate all lineages of the stomach

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epithelium. Cell 155: 357-368 (2013)

396) Petersen, N., Reimann, F., Bartfeld, S., Farin, H.F., Ringnalda, F.C., Vries, R.G., van den Brink, S., Clevers, H., Gribble, F.M., de Koning, E.J. Generation of L-cells in mouse and human small intestine organoids. Diabetes 63: 410-420 (2014)

397) de Groot, R.E., Farin, H.F., Macůrková, M., van Es, J.H., Clevers, H.C., Korswagen, H.C. Retromer dependent recycling of the Wnt secretion factor Wls is dispensable for stem cell maintenance in the mammalian intestinal epithelium. PLoS One 8: e76971(2013)

398) Schwank, G., Andersson-Rolf, A., Koo, B.K., Sasaki, N., Clevers, H. Generation of BAC Transgenic Epithelial Organoids. PLoS One 8: e76871 (2013)

399) Hirsch, D., Barker, N., McNeil, N., Hu, Y., Camps, J., McKinnon, K., Clevers, H., Ried, T., Gaiser,T. LGR5 positivity defines stem-like cells in colorectal cancer. Carcinogenesis (in press)

400) Yin, X., Farin, H.F., van Es, J.H., Clevers, H., Langer, R., Karp, J.M. Niche-independent high-purity cultures of Lgr5+ intestinal stem cells and their progeny. Nat Methods 11:106-112 (2014)

401) Bigorgne, A.E., Farin, H.F., Lemoine, R., Mahlaoui, N., Lambert, N., Gil, M., Schulz, A., Philippet, P., Schlesser, P., Abrahamsen, T.G., Oymar, K., Davies, E.G., Ellingsen, C.L., Leteurtre, E., Moreau-Massart, B., Berrebi, D., Bole-Feysot, C., Nischke, P., Brousse, N., Fischer, A., Clevers, H., de Saint Basile, G. TTC7A mutations disrupt intestinal epithelial apicobasal polarity. J Clin Invest. 124: 328-337 (2014)

402) Schwank, G., Koo, B.K., Sasselli, V., Dekkers, J.F., Heo, I., Demircan, T., Sasaki, N., Boymans, S., Cuppen, E., van der Ent, C.K., Nieuwenhuis, E.E., Beekman, J.M., Clevers, H. Functional Repair of CFTR by CRISPR/Cas9 in Intestinal Stem Cell Organoids of Cystic Fibrosis Patients. Cell Stem Cell 13: 653-658 (2013)

403) Mokry, M., Middendorp, S., Wiegerinck, C.L., Witte, M., Teunissen, H., Meddens, C.A., Cuppen, E., Clevers, H., Nieuwenhuis, E.E. Many Inflammatory Bowel Disease Risk Loci Include Regions that Regulate Gene Expression in Immune Cells and the Intestinal Epithelium. Gastroenterology 146:1040-1047 (2014)

404) Peng, W.C., de Lau, W., Madoori, P.K., Forneris, F., Granneman, J.C., Clevers, H., Gros, P. Structures of Wnt-Antagonist ZNRF3 and Its Complex with R-Spondin 1 and Implications for Signaling. PLoS One 8: e83110 (2013)

405) Snippert, H.J., Schepers, A.G., van Es, J.H., Simons, B.D., Clevers, H. Biased competition between Lgr5 intestinal stem cells driven by oncogenic mutation induces clonal expansion. EMBO Rep. 15: 62-69 (2014)

406) Schuijers, J., Mokry, M., Hatzis, P., Cuppen, E., Clevers, H. Wnt-induced transcriptional activation is exclusively mediated by TCF/LEF. EMBO J. 33:146-156 (2014)

407) Liu, X., Chen, X., Zhong, B., Wang, A., Wang, X., Chu, F., Nurieva, R.I., Yan, X., Chen, P., van der Flier, L.G., Nakatsukasa, H., Neelapu, S.S., Chen, W., Clevers, H., Tian, Q., Qi, H., Wei, L., Dong, C. Transcription factor achaete-scute homologue 2 initiates follicular T-helper-cell development. Nature (in press)

408) Sun, X., Terakawa, J., Clevers, H., Barker, N., Daikoku, T., Dey, S.K. Ovarian LGR5 is critical for successful pregnancy. FASEB J. (in press)

409) Middendorp, S., Schneeberger, K., Wiegerinck, C.L., Mokry, M., Akkerman, R.D., van Wijngaarden, S., Clevers, H., Nieuwenhuis, E.E. Adult stem cells in the small intestine are

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intrinsically programmed with their location-specific function. Stem Cells (in press)

410) Koo, B.K., Sasselli, V., Clevers, H. Retroviral gene expression control in primary organoid cultures. Curr Protoc Stem Cell Biol. 27:5A.6.1-8 (2013)

411) Ritsma, L., Ellenbroek, S.I., Zomer, A., Snippert, H.J., de Sauvage, F.J., Simons, B.D., Clevers, H., van Rheenen, J. Intestinal crypt homeostasis revealed at single-stem-cell level by in vivo live imaging. Nature 507: 362-365 (2014)

412) de Lau, W., Peng, W.C., Gros, P., Clevers, H. The R-spondin/Lgr5/Rnf43 module: regulator of Wnt signal strength. Review Genes Dev. 28: 305-316 (2014)

413) Sachs, N., Clevers, H. Organoid cultures for the analysis of cancer phenotypes. Review Curr Opin Genet Dev. 24C: 68-73 (2013)

414) Veleva-Rotse, B.O., Smart, J.L., Baas, A.F., Edmonds, B., Zhao, Z.M., Brown, A., Klug, L.R., Hansen, K., Reilly, G., Gardner, A.P., Subbiah, K., Gaucher, E.A., Clevers, H., Barnes, A.P. STRAD pseudokinases regulate axogenesis and LKB1 stability. Neural Dev. 9: 5 (2014)

415) Nakaya,T., Ogawa, S., Manabe, I., Tanaka, M., Sanada, M., Sato, T., Taketo, M.M., Nakao, K., Clevers, H., Fukayama, M., Kuroda, M., Nagai, R. KLF5 regulates the integrity and oncogenicity of intestinal stem cells. Cancer Res. (in press)

416) Andersson, R. et al. An atlas of active enhancers across human cell types and tissues. Nature 507: 455-461 (2014)

417) FANTOM Consortium and the RIKEN PMI and CLST (DGT). A promoter-level mammalian expression atlas. Nature 507 :462-470

418) Verhaegh, W., van Ooijen, H., Inda, M.A., Hatzis, P., Versteeg, R., Smid, M., Martens, J., Foekens, J., van de Wiel, P., Clevers, H., van de Stolpe, A. Selection of personalized patient therapy through the use of knowledge-based computational models that identify tumor-driving signal transduction pathways.

419) Cancer Res. (in press) 420) Wiegerinck, C.L., Janecke, A.R., Schneeberger, K., Vogel, G.F., van Haaften-Visser, D.Y.,

Escher, J.C., Adam, R., Thöni, C.E., Pfaller, K., Jordan, A.J., Weis, C.A., Nijman, I.J., Monroe, G.R., van Hasselt, P.M., Cutz, E., Klumperman, J., Clevers, H., Nieuwenhuis, E.E., Houwen, R.H., van Haaften, G., Hess, M.W., Huber, L.A., Stapelbroek, J.M., Müller, T., Middendorp, S. Loss of Syntaxin 3 Causes Variant Microvillus Inclusion Disease. Gastroenterology (in press)

Documents

--CURRICULUM VITAEbtc.wustl.edu/iscgt14/CVHansClevers.pdf · Curriculum vitae J.C. Clevers (1-5-2014) 14) Keynote Lecture at the Dutch-German meeting of Molecular Cardiology groups