CURRICULUM VITAE Name: Henry Joseph Donahue · CURRICULUM VITAE Name: Henry Joseph Donahue Address: Department of Biomedical Engineering School of Engineering Virginia Commonwealth

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CURRICULUM VITAE

Name: Henry Joseph Donahue

Address: Department of Biomedical Engineering School of Engineering Virginia Commonwealth University 401 West Main Street P.O. Box 843067 Richmond, VA 23284-3067

Phone: 804-828-3140 e-mail: [email protected]

Education: Degree Year Institution Major Field

B.S. 1979 San Diego State University Zoology M.A. 1984 University of California

Santa Barbara Biology Ph.D. 1986 University of California

Santa Barbara Biology Postdoctoral 1989 Mayo Graduate School of

Medicine, Rochester, MN. Endocrinology

Academic Appointments:

2016-present School of Engineering Foundation Professor and Chair Department of Biomedical Engineering School of Engineering, Virginia Commonwealth University

Richmond, VA.

2016-present Professor (affiliate) Department of Physiology and Biophysics School of Medicine, Virginia Commonwealth University Richmond, VA

2016-present Professor Emeritus of Orthopaedics Pennsylvania State University College of Medicine, Hershey, PA.

2016-present Team Leader, Musculoskeletal Alterations Team, National Space Biomedical Research Institute, Houston, TX

2012-2015 Associate Team Leader, Musculoskeletal Alterations Team,

National Space Biomedical Research Institute, Houston, TX.

2002-2015 Michael and Myrtle Baker Professor of Orthopaedics, Vice Chair for Research and

Director, Division of Musculoskeletal Sciences, Department of Orthopaedics & Rehabilitation, Professor, Department of Cellular & Molecular Physiology, Pennsylvania State University College of Medicine, Hershey, PA.

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2012-2015 Professor, Department of Biomedical Engineering

Pennsylvania State University, University Park, PA. 2008-2015 Faculty, Graduate Program in Anatomy, Penn State College of Medicine, PA. 1996-2015 Faculty, M.D./Ph.D. Program, Pennsylvania State University College of Medicine,

Hershey, PA. 2002-2012 Associate Faculty, Department of Bioengineering

Pennsylvania State University, University Park, PA. 2002-2011 Director, The Graduate Program in Cell and Molecular Biology, Pennsylvania State

University College of Medicine, Hershey, PA. 2000-2002 Professor, Departments of Orthopaedics & Rehabilitation and Cellular & Molecular

Physiology, Director, Musculoskeletal Research Laboratory, Pennsylvania State University College of Medicine, Hershey, PA.

1994-2000 Associate Professor, Departments of Orthopaedics & Rehabilitation and Cellular &

Molecular Physiology, Director, Musculoskeletal Research Laboratory, Pennsylvania State University College of Medicine, Hershey, PA.

1996-2006 Faculty, Life Science Consortium, Integrative Biosciences Graduate Program,

Biomolecular Transport Dynamics and Molecular Medicine Options, Pennsylvania State University, University Park, PA.

1993-1994 Assistant Professor, Department of Physiology and Biophysics (joint appointment), State University of New York, Stony Brook, NY.

1989-1994 Assistant Professor, Department of Orthopaedics, State University of New York,

Stony Brook, NY. 1988-1989 Instructor in Physiology, Mayo Medical School, Rochester, MN. 1986-1989 NIH Postdoctoral Trainee, Mayo Graduate School of Medicine, Rochester, MN.

Current Grants: Principal Investigator: Gap junctions and bone cell response to physical signals, NIH, 9R01AR068132-15A1 (formally 2R01AG13087) $1,642,102 for five years (years 15 to 20), 9/15/94-11/31/2019, 25% effort. Principal Investigator: Hydroxyapatite Nanotopographies to Enhance Allograft Osteointegration, Musculoskeletal Transplant Foundation (MTF), $299,185 for three years, 3/2/2014-2/28/2017. Principal Investigator: Somatic Mutations in Muscle and Bone Exposed to Simulated Space Radiation and Microgravity, NSBRI/NASA, 14-14NSBRI2-0003, $799,165 for two years. 05/1/2015-04/30/2017, 5% effort.

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Co-Investigator (PI: Niyibizi): Effect of Pigment Epithelium Derived Factor on Sclerostin Expression by Osteocytes, NIH, R21 for 2 years, 04/01/2015-03/31/2017, 2% effort.

Pending Grants: Principal Investigator: Personalized artificial bone constructs with nanoscale geometries, Department of Defense, $199,944 for 18 months, 07/01/2016-12/31/2017. Principal Investigator: Nanostructured Hydrogel Composites for Bone Regeneration, Department of Defense, $1,150,368.for 3 years, 07/01/2016-12/31/2019.

Previous Grant Support:

Principal Investigator: Integrated regulation of bone and muscle metabolism by simulated microgravity, NSBRI/NASA, MA02802, $1,186,763 for three years, 10/01/2012-09/30/2015, 10% effort. Principal Investigator: 12th International Bone Fluid Flow Workshop 2014, NIH, 1R13AR067632-01, for one year $15,000. 9/1/2014-8/30/2015. Principal Investigator: Biophysical signals, biomaterial surface characteristics and hMSC differentiation, NIH, R01AR54937, $1,804,168 for five years, 4/1/2009-3/31/2015, 20% effort (no cost extension). Co-Investigator: (PI: J.S. Reid): Expression of single nucleotide polymorphisms in delayed fracture healing. Orthopaedic Trauma Association, 01/01/2012-12/31/2013, $25,000, for one year, 1% effort. Principal Investigator: Induced Pluripotent stem cell-derived mesenchymal stem cells for attenuating age-related bone loss. Department of Defense. $116,324 for one year 7/1/2011 to 6/30/2012, 5% effort. Co-Investigator: (PI: Y. Zhang) Osteoblastic lineage cells regulate osteoclastogenesis via Cx43 and gap junctions, NIH, R03, 232,650 for three years, 7/01/2009-06/30/2012, 5% effort. Principal Investigator: Biomimetic materials to enhance bone graft healing, Musculoskeletal Transplant Foundation, $200,000 for two years, 01/01/2011-12/31/2012, 4% effort. Principal Investigator: The use of biomimetric hydroxyapatite nanotopographies to enhance allograft osteointegration. The Grace Woodward Collaborative Engineering/Medicine Research Program. $30,000 for one year. Co-Investigator: (PI: C. Jacobs) Mechanotransduction in bone via oscillating fluid flow, NIH, R01AR45989-09, $779,702 for four years, 3/31/05-4/1/11. Preceptor: (PI: J. Jefferson) Research training in physiological adaptation to stress, NIH, T32, $547,999 for five years, 4/1/96-3/31/07. Principal Investigator: Intercampus center for biomedical devices and functional tissue engineering, Pennsylvania State Tobacco Settlement Formula Fund (FTT) Award, $456,087 for two years, 6/4/04-6/30/08, 10% effort. Principal Investigator: Intercellular communication in breast cancer metastasis to bone, NIH, R01CA90991, $1,277,370 for five years, 4/01/01-3/31/07, 20% effort.Principal Investigator: Effects of Thrombin Analog on

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Osteoblastic Differentiation, OrthoLogic Inc., $166,380 for three years, 9/1/03-8/30/06, 1% effort. Co-Investigator: (P.I. S. Bronson) Murine osteoprogenitor formation and differentiation, NIH, R01AR47365, $1,149,325 for 5 years, 7/1/01-6/30/06, 10% effort. Principal Investigator: Cell membrane compliance and breast cancer cell metastatic potential, Project 3 of Breast cancer metastasis to bone (Danny Welch, P.I.) Pennsylvania State Tobacco Settlement Formula Fund (FTT) Award, $54,229 for two years, 6/4/02-6/30/04, 5% effort. Principal Investigator: Fluid flow sensitivity of bone cells as a function of age, US Army Medical Research and Material Command, $851,773 for 4 years, 4/1/98-8/31/02, 20% effort. Sponsor: The effects of extracellular nucleotides and fluid flow on osteoblastic cells. NIH-NRSA, post-doctoral fellowship for Jun You, Ph.D., F32AR049129, $104,456 for three years, 8/01/02-7/30/05. Co-Investigator: (PI: C. Davis) Regulation of cartilage callus formation during rat mandibular fractures healing, OREF, $100,000 for two years, 7/1/99-6/30/01, 10% effort. Principal Investigator: Gap junctional intercellular communication and breast cancer metastasis to bone, US Army Medical Research and Material Command, $78,300 for 1 year, 8/1/00-7/31/01, 5% effort. Principal Investigator: Fluid flow and stretch activated channels in chondrocytes, NIH, R01AG15107, $624,423 for four years, 1/1/98-12/31/01, 30% effort. Sponsor: Mechanotransduction pathways in chondrocyte biosynthesis, NIH-NRSA, post-doctoral fellowship for Clare Yellowley, Ph.D., F32AR08514, $98,460 for three years, 1/1/98-12/31/00. Co-Investigator: (PI: E. Rannels) Regulation of pulmonary alveolar epithelial cell gap junctions by extracellular matrix, American Heart Association, $164,964 for three years, 1/1/98-12/31/00. Co-Investigator: (PI: H.P. Ehrlich) Gap junctional communication in wound repair, NIH, R01GM56851, $15,000 for one year, 1/1/99-12/31/99, 10% effort. Co-Investigator: (PI: G. Deol) The cytosolic Ca2+ response of non-osteoarthritic and osteoarthritic chondrocytes to fluid flow-induced shear stress, Orthopaedic Research and Education Foundation, $14,891 for one year, 7/1/97-6/30/98. Principal Investigator: Age-related changes in bone cell signal transduction. NIH, R29AG10199, $527,017 for 5 years, 12/1/91-11/30/97, 50% effort. Co-Investigator: (PI: K. Black) The response of articular cartilage to resistance training, Laverty Foundation, $28,065 for one year, 11/1/96-10/31/97. Co-Investigator: (PI: K. McLeod) Cellular responses to low frequency electric and magnetic fields. Electrical Power Research Institute, $448,569 for 3 years, 1/1/91-12/31/93, 20% effort.

Co-Investigator: (PI: K. McLeod) Perturbation of normal cell processes by ELF electric fields, NIH, R01ES06287, $1,035,992 for 5 years, 11/1/92-10/31/94, 15% effort.

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Co-Investigator: (PI: B.Rifkin) Effect of tetracyclines on bone cell function In Vitro. NIH DE09576, $1,888,241 for 5 years, 7/1/90-6/30/92, B.Rifkin Principal Investigator, sub-contract to H.J. Donahue, $43,930 for 5 years, 5% effort. Co-Investigator: (PI: P. Levin) Identification of the biologically active component of ultrasound: optimization for bone cell stimulation. Orthopaedic Research and Education Foundation, $80,246 for 2 years, 3/1/90-2/28/92, 10% effort. Principal Investigator: Age-related changes in bone cell signal transduction, American Federation for Aging Research, $24,970 for 1 year, 7/1/90-6/30/91, 25% effort. Advisor: An In Vitro Model to study bone cell signal transduction: interaction of parathyroid hormone and estradiol, Howard Hughes Medical Institute Research Fellowship for Medical Students: Jose Zapatero, $20,600 for 1 year, 6/1/90-5/31/91.

Patents: Patent Pending: International Patent Cooperation Treaty (PCT) Application filed with the U.S. Patent and Trademark Office on January 24, 2013, and assigned Application Serial Number PCT/US2013/022999. Title: BIOMIMETIC SURFACES FOR ENHANCING MUSKULOSKELETAL REGENERATION Inventors: Henry J. Donahue and Akhlesh Lakhtakia

Honors and Awards: Orthopaedic Research Society Outstanding Achievement in Mentoring Award, 2017 Elected Fellow, American Institute for Medical and Biological Engineering (AIMBE), 2015 Elected to membership in the Mentoring Academy at Pennsylvania State University, 2004. International Union of Physiological Scientists Travel Award, 1997. International Conference on Calcium Regulating Hormones Travel Award, 1995. First Independent Research Support and Transition Award, National Institutes of Health, 1991. Grant-in-Aid of Research, Sigma Xl, The Scientific Research Society, New Haven, CT, 1985. Junior Investigator Research Grant, Innovative Research of America, Gaithersburg, MD, 1984. Graduate Fellowship for Pre-Doctoral Students, American Foundation for Aging Research, Columbia, MI, 1984. Jake Gimbel Scholarship Award, Bank of America, Los Angeles, CA, 1983. Graduate Student Research Grant, Graduate Division, University of CA, Santa Barbara, 1983. Patent Fund Award, Department of Biological Sciences, University of CA, Santa Barbara, 1983.

Committees and Public Service: National and International Community 2015- Member, Executive Committee, Indiana University Comprehensive Training

Program in Musculoskeletal Research 2015-2016 Member, Science Definition Team, Center for the Advancement of Science in

Space 2015 Chair, National Institutes of Health ZRG1 MOSS-U(90) Special Emphasis Panel 2015 Reviewer, National Institutes of Health Director’s Transformative Research Award 2013 Ad Hoc Member, National Institutes of Health Skeletal Biology Structure &

Regeneration Study Section 2012-present NASA Space Biology Development, Reproduction, and Epigenetics review panel

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2011-present Faculty, Orthopaedic Research Society/Orthopaedic Research and Education Foundation Grant Writing Workshop

2005-2012 Member of the Board of Directors, Orthopaedic Research Society 2009-2013 Member, Advisory Board, International Bone and Mineral Society Sun Valley

Workshop 2010 Member, Canadian Institutes of Health Research, Catalyst Award Review Board 2010 Ad Hoc Member, National Institutes of Health Skeletal Biology Structure &

Regeneration Study Section 2009 Ad Hoc Member, National Institutes of Health Tumor Microenvironment Study

Section 2009 Ad Hoc Member, National Institutes of Health Skeletal Biology Structure &

Regeneration Study Section 2009 Reviewer, NASA and National Space Biomedical Research Institute Post Doctoral

Fellowship Program 2009 Reviewer, Breast Cancer Program for the Department of Defense Congressionally

Directed Medical Research Programs 2005-2009 Secretary and Member of the Board of Directors, Orthopaedic Research Society 2009 Instructor, Tenth Annual ORS/OREF/AAOS Grant Writing Workshop 2003-2008 Regular Member, National Institutes of Health Tumor Microenvironment Study

Section 2008 Reviewer, NASA and National Space Biomedical Research Institute Post Doctoral

Fellowship Program 2008 Instructor, Ninth Annual ORS/OREF/AAOS Grant Writing Workshop 2000-2008 American Federation for Aging Research National Scientific Advisory Committee 1996-2008 External Referee, Medical Research Council of Canada 1992-2008 Consultant, Department of Veterans Affairs Merit Review 2007 Chair, National Institutes of Health, Bone Cell Biology Study Section 2005 Chair, National Institutes of Health, Osteoporosis: Molecular and Cellular

Mechanisms Special Study Section 2002 Member, National Institutes of Health Reparative Medicine Study Section 2002 Member, Orthopaedic Research Society Program Committee 2001 Chair, Osteocytes: Role in cell signaling mechanical stimulus detection in bone

session, 31st International Sun Valley Hard Tissue Workshop. 2001 External Reviewer, Canada Foundation for Innovation 2000 Member, NIH NIDR Special Emphasis Panel 2000 Chairperson, NIH Center for Scientific Review Special Emphasis Panel 1996-2000 Regular Member, National Institutes of Health Orthopaedics and Musculoskeletal

Study Section 2000 External Referee, Arthritis Society of Canada 2001 Chairman, Growth Factors, BMP’s and Cytokines Session, 45th Annual Meeting of

the Orthopaedic Research Society 1999 Member, NIH Special Emphasis Panel, NIGMS, Trauma and Burn Injury Research

Center Grant 1998 Chairman, Bone Biology Session, 43rd Annual Meeting of the Orthopaedic Research

Society 1997-1999 Adjunct Reviewer, Orthopaedic Research Society Program Committee 1997 Chairman, Extracellular Matrix Dynamics in Health and Disease, Experimental

Biology 97, New Orleans, LA

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1996 Ad Hoc Member, National Institutes of Health Orthopaedics and Musculoskeletal Study Section

1996 Peer Reviewer, United States Army Osteoporosis Research Program 1995-1996 Member, National Institutes of Health Oral Medicine Special Study Section 1995 Ad Hoc Member, National Institutes of Health Oral Medicine B Study Section 1996 Ad Hoc Member, National Institutes of Health Orthopaedics and Musculoskeletal

Study Section 1994-1998 Public Affairs Committee, American Society for Bone and Mineral Research 1994 Ad Hoc Reviewer, Faculty Development Initiative, Faculty of Dentistry,

The University of Western Ontario, Canada 1994 Ad Hoc Reviewer, West Virginia University Faculty Research Grant 1993 Chairman, Osteoblast Session, 15th Annual Meeting of the American Society for

Bone and Mineral Research, Tampa, Florida 1993 Outside Reviewer, National Institutes of Health, IDeA Program Application 1992 Moderator, Calcium Signal Transduction Session, First World Congress for

Electricity & Magnetism in Biology and Medicine, Lake Buena Vista, Florida. Virginia Commonwealth University Community 2016-present Member, Internal Advisory Board, VCU Weil Institute Penn State University Community 2013-2015 Member, Research Team, Pennsylvania State Unified Campus Teams 2013-2015 Member, Promotion and Tenure Committee, Penn State College of Medicine 2006-2012 Senator, University Faculty Senate 2003-2015 Technology Fund Reviewer, Life Sciences Green House of Central Pennsylvania 2001-2015 Member, Penn State College of Medicine Scientific Review Committee 2010-2012 Member, Promotion and Tenure Committee, Penn State College of Medicine 2007-2009 Member, Physical Resources Team, Pennsylvania State Unified Campus Teams 2007-2008 Member, Penn State Task Force on Interdisciplinary Strategies in Graduate

Education 2007-2008 Member, University Faculty Senate Research Committee 1999-2010 Chair, Department of Orthopaedics and Rehabilitation Academic Promotion and

Tenure Committee, PSU College of Medicine 2003-2005 Member, Steering Committee, Huck Life Science Institute, Pennsylvania State

University 2002 Member, Pennsylvania State University College of Medicine Site & Facilities Master

Plan Steering Committee 2001 Member, Committee to Review Bioengineering at Pennsylvania State University 2001 Member, Chair of Department of Orthopaedics and Rehabilitation Search Committee 2001 Member, Vice Dean for Research Search Committee, Pennsylvania State University

College of Medicine 2000-2004 Member, Research Team, Pennsylvania State Unified Campus Teams 2000-2004 Member, Graduate Council, Pennsylvania State University 1996 Member, Committee to Review the Research Aspect of the Center for Sports

Medicine, University Park 1995 Member, Committee to Develop Guidelines for Joint Faculty Appointments in the

Department of Cellular and Molecular Physiology, College of Medicine 1994 Demonstrator, Howard Hughes Medical Institute Precollege Science Initiative for Biomedical Research Institutes

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Stony Brook University Community 1991-1994 Resident Selection Committee, Dept. of Orthopaedics, S.U.N.Y. @ Stony Brook,

New York 1991-1994 University-wide Faculty Senate, S.U.N.Y. @ Stony Brook, New York 1991-1994 Faculty Senate, School of Medicine, S.U.N.Y. @ Stony Brook, New York 1989-1994 Research Committee, Dept. of Orthopaedics, S.U.N.Y. @ Stony Brook, New York 1989-1994 Curriculum Committee, Dept. of Orthopaedics, S.U.N.Y. @ Stony Brook, New York Local Community 2008-present Publically elected member, Board of School Directors, Derry Township School

District, Hershey, PA.

Editorial Positions: Member, Editorial Board, The American Journal of Physiology - Endocrinology and Metabolism Chair, Editorial Advisory Board, the Journal of Orthopaedic Research Member, Editorial Board, Journal of Bone and Mineral Research

Acta Acta Biomaterialia

Endocrinology American Journal of Physiology Arthritis and Rheumatism BioEssays Bone European Journal of Clinical Investigation European Journal of Experimental Musculoskeletal Research Journal of Applied Physiology Journal of Biological Chemistry Journal of Biomaterials Research Journal of Biomechanics Journal of Bone and Mineral Research Journal of Cellular Biochemistry Journal of Cellular Physiology Journal of Orthopaedic Research Life Sciences Metabolism Nanomedicine PLoS One The Histochemical Journal

Teaching Responsibilities: Penn State 1999-Present Lecturer, Bone Cartilage Biology, Musculoskeletal Block 2008-2012 Lecturer, BIOE 519 Artificial Organs

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1999-2010 Co-Director, CMBIO 540 Core Cell Biology Course (now BMS 502, Cell and Systems Biology)

1999-2005 Lecturer, CMBIO 540 Core Cell Biology Course 2000-2002 Director, IBIOS 598F Cellular Transport Dynamics 1997-2002 Facilitator, Block IV, Problem Based Learning 1996-2000 Lecturer, Advanced Topics in Cellular and Molecular Physiology 1994-2004 Director, Department of Orthopaedics Basic Science Course 1995-1998 Mentor, Howard Hughes Medical Institute Summer Research Program Stony Brook 1990-1994 Mentor for Howard Hughes Medical Institute Medical Student Fellowship awarded to

Jose Zapatero 1989-1994 "Musculo-Skeletal Physiology" Lecturer, Dept. of Medicine,1989-1994 "Bone and

Connective Tissue, New Perspectives of Basic Concepts" Lecturer, Dept. of Orthopaedic Surgery

1989-1994 "Bone Physiology" Lecturer, Dept. of Surgery Mayo Graduate School of Medicine 1988-1989 "Human Physiology" Lecturer, University of California Santa Barbara 1984-1985 "Hormone Pharmacology" Lecturer, Dept. of Biology.

Post-Doctoral Fellows, Graduate and Medical Students: At Stony Brook Jose Zapatero, M.D., with Distinction in Research Gregg Geiger, M.D., Orthopaedic Resident Christian Dee, M.D., Orthopaedic Resident Free Lee, M.D., with Distinction in Research David Loya, M.D., Orthopaedic Resident

At Penn State Previous Orthopaedic Residents and Medical Students Todd Campbell, M.D. Joe Conway, M.D. Mark Raybeck, M.D. Mark Mason, M.D., Orthopaedic Resident Gurvinder Deol, M.D., Orthopaedic Resident Karen Huang, M.D., Orthopaedic Resident Tamala Pylawka, M.D., Orthopaedic Resident Vikram Sathyendra, M.D., Orthopaedic Resident

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Previous Graduate Students Monique Van der Molen-Bibb, Ph.D. Physiology (thesis advisor) Kathy Buhl, Ph.D., Kinesiology (thesis advisor) Andrea Lesulauro, Ph.D., Cellular and Molecular Physiology (member of thesis committee) Yong Chang, Ph.D., Bioengineering (member of thesis committee) Ritu Sharma, MS, Bioengineering (member of thesis committee) Josh Hansen, M.S., Bioengineering (thesis advisor) Michael Jekir, M.S., Bioengineering (thesis advisor) Xiaomei Liu, Ph.D., Bioengineering (member of thesis committee) Damian Genetos, Ph.D., Cellular and Molecular Physiology (thesis advisor) Geetha Babu, Ph.D.,Cell and Molecular Biology (member of thesis committee) Melanie Lieby, Ph.D., Cellular and Molecular Biology (Chair of thesis committee) Nicole Woll, Ph.D., Molecular Medicine (member of thesis committee) Ryan Riddle, Ph.D., Cell and Molecular Biology (thesis advisor) Eleanor Hernandez, M.S. Physiology (member of thesis committee) Jiyao Zou, Ph.D., Cellular and Molecular Physiology (thesis advisor) Sung Jin Huh, Ph.D. Cell and Molecular Biology (member of thesis committee) Jackie Yanoso, Ph.D. Biochemistry and Molecular Biology(member of thesis committee) Yanghui Xing, Ph.D. Candidate, Bioengineering (member of thesis committee) Xianming Wang, Ph.D. Cellular and Molecular Physiology (member of thesis committee) Rachelle Schenk, M.S. Anatomy, (thesis advisor) Lai Wei, Ph.D., Engineering Science and Mechanics (member of thesis committee) Sarah Sheehan, Ph.D. Anatomy, (member of thesis committee) Karis Chin-Quee Ph.D., Cellular and Molecular Physiology (thesis advisor) Christopher Schank, M.S. Anatomy, (member of thesis committee) Jason Shaumberg, M.S. Anatomy, (member of thesis committee) Shane Specht, M.S. Anatomy, (member of thesis committee) Miguel Barthelery, Ph.D. Candidate, Molecular Medicine (member of thesis committee) Walter Jackson III. Ph.D. Candidate, Immunology & Infectious Diseases, (member of thesis committee). Peter Govey, Ph.D. Candidate, Bioengineering (thesis advisor) Shane Lloyd, MD/Ph.D. Candidate, Cell and Molecular Biology (thesis advisor)

Current Graduate Students Andrew Krause, Ph.D. Candidate, Anatomy (thesis advisor) Toni Coringrato, Ph.D. Candidate, Physiology (thesis advisor) Previous Post-doctoral Fellows Clare Yellowley, Ph.D., currently Professor, University of California, Davis Maximillian Edlich, M.D., Ph.D. currently Orthopaedic Surgeon in Germany Kazutoshi Kurokouchi, M.D., Ph.D., currently Orthopaedic Surgeon in Japan Marnie Saunders, Ph.D., currently Associate Professor, University of Akron Seth Donahue, Ph.D., currently Associate Professor, Colorado State University Andrea Alford, Ph.D., currently Assistant Professor, University of Michigan Jun You, Ph.D., currently Assistant Professor, Pennsylvania State University Preeti Kapoor, Ph.D., currently post-doctoral fellow in India Amanda Taylor, Ph.D., currently Scientist, MED Institute, Inc. West Lafayette, Indiana Jung Lim, Ph.D., currently Assistant Professor, University of Nebraska Yue Zhang, Ph.D., currently Assistant Professor, Pennsylvania State University Alayna Loiselle, Ph.D. currently Assistant Professor, University of Rochester

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At Virginia Commonwealth University

Current Undergraduate Students Christopher Brunkhorst Current Graduate Students Otto Juhl (thesis advisor) Alex Collins (thesis advisor) Ryan Clohessy (member of thesis committee) Kelly Hotchkiss (member of thesis committee) Current Post-doctoral fellows Seyed Mohsen Latifi, Ph.D.

Society Memberships American Association for the Advancement of Science American Society for Bone and Mineral Research Orthopaedic Research Society International Bone and Mineral Society

Dissertation

Donahue, H.J. The effect of age and estrogen status on bone and vitamin D metabolism in female rats (1986) Ph.D. Dissertation, University of California, Santa Barbara.

Publications (as of July 2016 H-factor = 45, average citations per year >200)

1. Donahue HJ, Mazzeo RS, Horvath SM. Endurance training and bone loss in calcium-deficient and ovariectomized rats. Metabolism. 1988 Aug;37(8):741-4. PubMed PMID: 3405091.

2. Donahue HJ, Fryer MJ, Eriksen EF, Heath H 3rd. Differential effects of parathyroid hormone and its analogues on cytosolic calcium ion and cAMP levels in cultured rat osteoblast-like cells. J Biol Chem. 1988 Sep 25;263(27):13522-7. PubMed PMID: 2843523.

3. Donahue HJ, Penniston JT, Heath H 3rd. Kinetics of erythrocyte plasma membrane (Ca2+, Mg2+)ATPase in familial benign hypercalcemia. J Clin Endocrinol Metab. 1989 May;68(5):893-8. PubMed PMID: 2523897.

4. Donahue HJ, Fryer MJ, Heath H 3rd. Structure-function relationships for full-length recombinant parathyroid hormone-related peptide and its amino-terminal fragments: effects on cytosolic calcium ion mobilization and adenylate cyclase activation in rat osteoblast-like cells. Endocrinology. 1990 Mar;126(3):1471-7. PubMed PMID: 2307114.

5. Bidwell JP, Fryer MJ, Firek AF, Donahue HJ, Heath H 3rd. Desensitization of rat osteoblast-like cells (ROS 17/2.8) to parathyroid hormone uncouples the adenosine 3',5'-monophosphate and cytosolic ionized calcium response limbs. Endocrinology. 1991 Feb;128(2):1021-8. PubMed PMID: 1846574.

http://www.ncbi.nlm.nih.gov/pubmed/3405091/





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6. Donahue HJ, Iijima K, Goligorsky MS, Rubin CT, Rifkin BR. Regulation of cytoplasmic calcium concentration in tetracycline-treated osteoclasts. J Bone Miner Res. 1992 Nov;7(11):1313-8. PubMed PMID: 1466256.

7. McLeod KJ, Donahue HJ, Levin PE, Fontaine MA, Rubin CT. Electric fields modulate bone cell function in a density-dependent manner. J Bone Miner Res. 1993 Aug;8(8):977-84. PubMed PMID: 8213260.

8. Greenfield EM, Gornik SA, Horowitz MC, Donahue HJ, Shaw SM. Regulation of cytokine expression in osteoblasts by parathyroid hormone: rapid stimulation of interleukin-6 and leukemia inhibitory factor mRNA. J Bone Miner Res. 1993 Oct;8(10):1163-71. PubMed PMID: 8256653.

9. Rubin CT, Donahue HJ, Rubin JE, McLeod KJ. Optimization of electric field parameters for the control of bone remodeling: exploitation of an indigenous mechanism for the prevention of osteopenia. J Bone Miner Res. 1993 Dec;8 Suppl 2:S573-81. PubMed PMID: 8122529.

10. Donahue HJ, Brink PR, McLeod KJ, Hertzberg EL, Rubin CT. Age-induced attenuation of hormonally regulated intercellular communication in bone cell networks. Progress in Cell Researsh. 1995;4:349-52.

11. McLeod RJ, Rubin CT, Donahue HJ. Electromagnetic fields in bone repair and adaptation. Radio Science. 1995;30(1):233-44. DOI 10.1029/94RS00832

12. Donahue HJ, McLeod KJ, Rubin CT, Andersen J, Grine EA, Hertzberg EL, Brink PR. Cell-to-cell communication in osteoblastic networks: cell line-dependent hormonal regulation of gap junction function. J Bone Miner Res. 1995 Jun;10(6):881-9. PubMed PMID: 7572312.

13. Donahue HJ, Guilak F, Vander Molen MA, McLeod KJ, Rubin CT, Grande DA, Brink PR. Chondrocytes isolated from mature articular cartilage retain the capacity to form functional gap junctions. J Bone Miner Res. 1995 Sep;10(9):1359-64. PubMed PMID: 7502708.

14. Vander Molen MA, Rubin CT, McLeod KJ, McCauley LK, Donahue HJ. Gap junctional intercellular communication contributes to hormonal responsiveness in osteoblastic networks. J Biol Chem. 1996 May 24;271(21):12165-71. PubMed PMID: 8647809.

15. Lee YC, Yellowley CE, Li Z, Donahue HJ, Rannels DE. Expression of functional gap junctions in cultured pulmonary alveolar epithelial cells. Am J Physiol. 1997 Jun;272(6 Pt 1):L1105-14. PubMed PMID: 9227511.

16. Su M, Borke JL, Donahue HJ, Li Z, Warshawsky NM, Russell CM, Lewis JE. Expression of connexin 43 in rat mandibular bone and periodontal ligament (PDL) cells during experimental tooth movement. J Dent Res. 1997 Jul;76(7):1357-66. PubMed PMID: 9207768.

17. Yellowley CE, Jacobs CR, Li Z, Zhou Z, Donahue HJ. Effects of fluid flow on intracellular calcium in bovine articular chondrocytes. Am J Physiol. 1997 Jul;273(1 Pt 1):C30-6. PubMed PMID: 9252439.











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18. Donahue HJ, Zhou Z, Li Z, McCauley LK. Age-related decreases in stimulatory G protein-coupled adenylate cyclase activity in osteoblastic cells. Am J Physiol. 1997 Oct;273(4 Pt 1):E776-81. PubMed PMID: 9357808.

19. Donahue HJ, Vander Molen MA, Yellowley CE, Li Z. Gap junctional intercellular communication in bone. Cells and Materials. 1997; 6(13)10-20.

20. Donahue HJ. Gap junctional intercellular communication in bone: a cellular basis for the mechanostat set point. Calcif Tissue Int. 1998 Feb;62(2):85-8. PubMed PMID: 9437038.

21. Jacobs CR, Yellowley CE, Davis BR, Zhou Z, Cimbala JM, Donahue HJ. Differential effect of steady versus oscillating flow on bone cells. J Biomech. 1998 Nov;31(11):969-76. PubMed PMID: 9880053; PubMed Central PMCID: PMC3057628.

22. Bowman NN, Donahue HJ, Ehrlich HP. Gap junctional intercellular communication contributes to the contraction of rat osteoblast populated collagen lattices. J Bone Miner Res. 1998 Nov;13(11):1700-6. PubMed PMID: 9797478.

23. Guilak F, Zell RA, Erickson GR, Grande DA, Rubin CT, McLeod KJ, Donahue HJ. Mechanically induced calcium waves in articular chondrocytes are inhibited by gadolinium and amiloride. J Orthop Res. 1999 May;17(3):421-9. PubMed PMID: 10376733.

24. Yellowley CE, Jacobs CR, Donahue HJ. Mechanisms contributing to fluid-flow-induced Ca2+ mobilization in articular chondrocytes. J Cell Physiol. 1999 Sep;180(3):402-8. PubMed PMID: 10430180.

25. Li Z, Zhou Z, Yellowley CE, Donahue HJ. Inhibiting gap junctional intercellular communication alters expression of differentiation markers in osteoblastic cells. Bone. 1999 Dec;25(6):661-6. PubMed PMID: 10593410.

26. Samant RS, Seraj MJ, Saunders MM, Sakamaki TS, Shevde LA, Harms JF, Leonard TO,

Goldberg SF, Budgeon L, Meehan WJ, Winter CR, Christensen ND, Verderame MF, Donahue

HJ, Welch DR. Analysis of mechanisms underlying BRMS1 suppression of metastasis. Clin Exp Metastasis. 2000;18(8):683-93. PubMed PMID: 11827072.

27. Jacobs CR, Yellowley CE, Nelson DV, Donahue HJ. Analysis of Time-Varying Biological Data Using Rainflow Cycle Counting. Comput Methods Biomech Biomed Engin. 2000;3(1):31-40. PubMed PMID: 11264837.

28. Malecki EA, Buhl KM, Beard JL, Jacobs CR, Connor JR, Donahue HJ. Bone structural and mechanical properties are affected by hypotransferrinemia but not by iron deficiency in mice. J Bone Miner Res. 2000 Feb;15(2):271-7. PubMed PMID: 10703928.

29. Yellowley CE, Li Z, Zhou Z, Jacobs CR, Donahue HJ. Functional gap junctions between osteocytic and osteoblastic cells. J Bone Miner Res. 2000 Feb;15(2):209-17. PubMed PMID: 10703922.

30. Donahue HJ, Li Z, Zhou Z, Yellowley CE. Differentiation of human fetal osteoblastic cells and gap junctional intercellular communication. Am J Physiol Cell Physiol. 2000 Feb;278(2):C315-22. PubMed PMID: 10666026.




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31. Donahue HJ. Gap junctions and biophysical regulation of bone cell differentiation. Bone. 2000 May;26(5):417-22. PubMed PMID: 10773579.

32. You J, Yellowley CE, Donahue HJ, Zhang Y, Chen Q, Jacobs CR. Substrate deformation levels associated with routine physical activity are less stimulatory to bone cells relative to loading-induced oscillatory fluid flow. J Biomech Eng. 2000 Aug;122(4):387-93. PubMed PMID: 11036562.

33. Vander Molen MA, Donahue HJ, Rubin CT, McLeod KJ. Osteoblastic networks with deficient coupling: differential effects of magnetic and electric field exposure. Bone. 2000 Aug;27(2):227-31. PubMed PMID: 10913915.

34. Edlich M, Yellowley CE, Jacobs CR, Donahue HJ. Oscillating fluid flow regulates cytosolic calcium concentration in bovine articular chondrocytes. J Biomech. 2001 Jan;34(1):59-65. PubMed PMID: 11425081.

35. Guo Y, Martinez-Williams C, Yellowley CE, Donahue HJ, Rannels DE. Connexin expression by alveolar epithelial cells is regulated by extracellular matrix. Am J Physiol Lung Cell Mol Physiol. 2001 Feb;280(2):L191-202. PubMed PMID: 11158997.

36. Saunders MM, Seraj MJ, Li Z, Zhou Z, Winter CR, Welch DR, Donahue HJ. Breast cancer metastatic potential correlates with a breakdown in homospecific and heterospecific gap junctional intercellular communication. Cancer Res. 2001 Mar 1;61(5):1765-7. PubMed PMID: 11280719.

37. Buhl KM, Jacobs CR, Turner RT, Evans GL, Farrell PA, Donahue HJ. Aged bone displays an increased responsiveness to low-intensity resistance exercise. J Appl Physiol (1985). 2001 Apr;90(4):1359-64. PubMed PMID: 11247935.

38. You J, Reilly GC, Zhen X, Yellowley CE, Chen Q, Donahue HJ, Jacobs CR. Osteopontin gene regulation by oscillatory fluid flow via intracellular calcium mobilization and activation of mitogen-activated protein kinase in MC3T3-E1 osteoblasts. J Biol Chem. 2001 Apr 20;276(16):13365-71. PubMed PMID: 11278573.

39. Kurokouchi K, Jacobs CR, Donahue HJ. Oscillating fluid flow inhibits TNF-alpha -induced NF-kappa B activation via an Ikappa B kinase pathway in osteoblast-like UMR106 cells. J Biol Chem. 2001 Apr 20;276(16):13499-504. PubMed PMID: 11096064.

40. Donahue SW, Jacobs CR, Donahue HJ. Flow-induced calcium oscillations in rat osteoblasts are age, loading frequency, and shear stress dependent. Am J Physiol Cell Physiol. 2001 Nov;281(5):C1635-41. PubMed PMID: 11600427.

41. Saunders MM, You J, Trosko JE, Yamasaki H, Li Z, Donahue HJ, Jacobs CR. Gap junctions and fluid flow response in MC3T3-E1 cells. Am J Physiol Cell Physiol. 2001 Dec;281(6):C1917-25. PubMed PMID: 11698250.

42. Yellowley CE, Hancox JC, Donahue HJ. Effects of cell swelling on intracellular calcium and membrane currents in bovine articular chondrocytes. J Cell Biochem. 2002;86(2):290-301. PubMed PMID: 12111998.













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43. Shevde LA, Samant RS, Goldberg SF, Sikaneta T, Alessandrini A, Donahue HJ, Mauger DT, Welch DR. Suppression of human melanoma metastasis by the metastasis suppressor gene, BRMS1. Exp Cell Res. 2002 Feb 15;273(2):229-39. PubMed PMID: 11822878.

44. Sharma R, Yellowley CE, Civelek M, Ainslie K, Hodgson L, Tarbell JM, Donahue HJ. Intracellular calcium changes in rat aortic smooth muscle cells in response to fluid flow. Ann Biomed Eng. 2002 Mar;30(3):371-8. PubMed PMID: 12051621.

45. You J, Jacobs CR, Steinberg TH, Donahue HJ. P2Y purinoceptors are responsible for oscillatory fluid flow-induced intracellular calcium mobilization in osteoblastic cells. J Biol Chem. 2002 Dec 13;277(50):48724-9. PubMed PMID: 12376532.

46. Buhl KM, Jacobs CR, Turner RT, Evans GL, Farrell PA, Donahue HJ. Parallel changes in

extracellular matrix protein gene expression, bone formation and biomechanical properties in aging rat bone. J Musculoskeletal Res. 2002;6(3&4) 157-169. doi: 10.1142/S0218957702000848.

47. Reilly GC, Haut TR, Yellowley CE, Donahue HJ, Jacobs CR. Fluid flow induced PGE2 release by bone cells is reduced by glycocalyx degradation whereas calcium signals are not. Biorheology. 2003;40(6):591-603. PubMed PMID: 14610310.

48. Donahue SW, Donahue HJ, Jacobs CR. Osteoblastic cells have refractory periods for fluid-flow-induced intracellular calcium oscillations for short bouts of flow and display multiple low-magnitude oscillations during long-term flow. J Biomech. 2003 Jan;36(1):35-43. PubMed PMID: 12485636.

49. Donahue SW, Vaughan MR, Demers LM, Donahue HJ. Serum markers of bone metabolism show bone loss in hibernating bears. Clin Orthop Relat Res. 2003 Mar;PubMed PMID: 12616074.

50. Lohmann CH, Schwartz Z, Liu Y, Li Z, Simon BJ, Sylvia VL, Dean DD, Bonewald LF, Donahue

HJ, Boyan BD. Pulsed electromagnetic fields affect phenotype and connexin 43 protein expression in MLO-Y4 osteocyte-like cells and ROS 17/2.8 osteoblast-like cells. J Orthop Res. 2003 Mar;21(2):326-34. PubMed PMID: 12568966.

51. Welch DR, Harms JF, Mastro AM, Gay CV, Donahue HJ. Breast cancer metastasis to bone: evolving models and research challenges. J Musculoskelet Neuronal Interact. 2003 Mar;3(1):30-8. PubMed PMID: 15758363.

52. Saunders MM, You J, Zhou Z, Li Z, Yellowley CE, Kunze EL, Jacobs CR, Donahue HJ. Fluid flow-induced prostaglandin E2 response of osteoblastic ROS 17/2.8 cells is gap junction-mediated and independent of cytosolic calcium. Bone. 2003 Apr;32(4):350-6. PubMed PMID: 12689677.

53. Donahue HJ, Saunders MM, Li Z, Mastro AM, Gay CV, Welch DR. A potential role for gap junctions in breast cancer metastasis to bone. J Musculoskelet Neuronal Interact. 2003 Jun;3(2):156-61. PubMed PMID: 15758356.

54. Alford AI, Jacobs CR, Donahue HJ. Oscillating fluid flow regulates gap junction communication in osteocytic MLO-Y4 cells by an ERK1/2 MAP kinase-dependent mechanism. Bone. 2003 Jul;33(1):64-70. PubMed PMID: 12919700.












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55. Donahue TL, Haut TR, Yellowley CE, Donahue HJ, Jacobs CR. Mechanosensitivity of bone cells to oscillating fluid flow induced shear stress may be modulated by chemotransport. J Biomech. 2003 Sep;36(9):1363-71. PubMed PMID: 12893045.

56. Alford AI, Yellowley CE, Jacobs CR, Donahue HJ. Increases in cytosolic calcium, but not fluid flow, affect aggrecan mRNA levels in articular chondrocytes. J Cell Biochem. 2003 Dec 1;90(5):938-44. PubMed PMID: 14624453.

57. Donahue SW, Vaughan MR, Demers LM, Donahue HJ. Bone formation is not impaired by hibernation (disuse) in black bears Ursus americanus. J Exp Biol. 2003 Dec;206(Pt 23):4233-9. PubMed PMID: 14581593.

58. Lim JY, Donahue HJ, Kim SY. Strain rate, temperature, and microstructure dependent yield stress of Poly (Ethylene Terephthalate). Macromol Chem Phys 2003;204, 653-660.

59. Brown KM, Saunders MM, Kirsch T, Donahue HJ, Reid JS. Effect of COX-2-specific inhibition on fracture-healing in the rat femur. J Bone Joint Surg Am. 2004 Jan;86-A(1):116-23. PubMed PMID: 14711953.

60. Edlich M, Yellowley CE, Jacobs CR, Donahue HJ. Cycle number and waveform of fluid flow affect bovine articular chondrocytes. Biorheology. 2004;41(3-4):315-22. PubMed PMID: 15299264.

61. Harms JF, Welch DR, Samant RS, Shevde LA, Miele ME, Babu GR, Goldberg SF, Gilman VR,

Sosnowski DM, Campo DA, Gay CV, Budgeon LR, Mercer R, Jewell J, Mastro AM, Donahue HJ, Erin N, Debies MT, Meehan WJ, Jones AL, Mbalaviele G, Nickols A, Christensen ND, Melly R, Beck LN, Kent J, Rader RK, Kotyk JJ, Pagel MD, Westlin WF, Griggs DW. A small molecule antagonist of the alpha(v)beta3 integrin suppresses MDA-MB-435 skeletal metastasis. Clin Exp Metastasis. 2004;21(2):119-28. PubMed PMID: 15168729.

62. Mastro AM, Gay CV, Welch DR, Donahue HJ, Jewell J, Mercer R, DiGirolamo D, Chislock EM, Guttridge K. Breast cancer cells induce osteoblast apoptosis: a possible contributor to bone degradation. J Cell Biochem. 2004 Feb 1;91(2):265-76. PubMed PMID: 14743387.

63. Lim JY, Liu X, Vogler EA, Donahue HJ. Systematic variation in osteoblast adhesion and phenotype with substratum surface characteristics. J Biomed Mater Res A. 2004 Mar 1;68(3):504-12. PubMed PMID: 14762930.

64. Saunders MM, Donahue HJ. Development of a cost-effective loading machine for biomechanical evaluation of mouse transgenic models. Med Eng Phys. 2004 Sep;26(7):595-603. PubMed PMID: 15271287.

65. Haut Donahue TL, Genetos DC, Jacobs CR, Donahue HJ, Yellowley CE. Annexin V disruption impairs mechanically induced calcium signaling in osteoblastic cells. Bone. 2004 Sep;35(3):656-63. PubMed PMID: 15336601.

66. Kapoor P, Saunders MM, Li Z, Zhou Z, Sheaffer N, Kunze EL, Samant RS, Welch DR, Donahue

HJ. Breast cancer metastatic potential: correlation with increased heterotypic gap junctional intercellular communication between breast cancer cells and osteoblastic cells. Int J Cancer. 2004 Sep 20;111(5):693-7. PubMed PMID: 15252837.












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67. Lim JY, Donahue HJ. Biomaterial characteristics important to skeletal tissue engineering. J Musculoskelet Neuronal Interact. 2004 Dec;4(4):396-8. PubMed PMID: 15758276.

68. Donahue HJ. Summary--bone metastasis. J Musculoskelet Neuronal Interact. 2004 Dec;4(4):381-2. PubMed PMID: 15758270.

69. Oki A, Parveen B, Hossain S, Adeniji S, Donahue HJ. Preparation and in vitro bioactivity of zinc

containing sol-gel-derived bioglass materials. J Biomed Mater Res. 2004;69A: 216-21. PMID: 15057994.

70. Milner KR, Balmer M, Donahue HJ, Snyder AJ, Siedlecki CA. Fabrication of ordered sub-micron

topographies on large area poly(Urethane Urea) by two-stage replication molding. Materials Research Society Symposium Proceedings. 2004,820. R2.8.1-R2.8.6.

71. Kim YS, Lim JY, Donahue HJ, Lowe TL. Thermoresponsive terpolymeric films applicable for osteoblastic cell growth and noninvasive cell sheet harvesting. Tissue Eng. 2005 Jan-Feb;11(1-2):30-40. PubMed PMID: 15738659.

72. Lim JY, Taylor AF, Li Z, Vogler EA, Donahue HJ. Integrin expression and osteopontin regulation in human fetal osteoblastic cells mediated by substratum surface characteristics. Tissue Eng. 2005 Jan-Feb;11(1-2):19-29. PubMed PMID: 15738658.

73. Genetos DC, Geist DJ, Liu D, Donahue HJ, Duncan RL. Fluid shear-induced ATP secretion mediates prostaglandin release in MC3T3-E1 osteoblasts. J Bone Miner Res. 2005 Jan;20(1):41-9. PubMed PMID: 15619668; PubMed Central PMCID: PMC2929123.

74. Lim JY, Hansen JC, Siedlecki CA, Runt J, Donahue HJ. Human foetal osteoblastic cell response to polymer-demixed nanotopographic interfaces. J R Soc Interface. 2005 Mar 22;2(2):97-108. PubMed PMID: 16849169; PubMed Central PMCID: PMC1578253.

75. Lim JY, Hansen JC, Siedlecki CA, Hengstebeck RW, Cheng J, Winograd N, Donahue HJ. Osteoblast adhesion on poly(L-lactic acid)/polystyrene demixed thin film blends: effect of nanotopography, surface chemistry, and wettability. Biomacromolecules. 2005 Nov-Dec;6(6):3319-27. PubMed PMID: 16283761.

76. Saunders MM, Taylor AF, Du C, Zhou Z, Pellegrini VD Jr, Donahue HJ. Mechanical stimulation effects on functional end effectors in osteoblastic MG-63 cells. J Biomech. 2006;39(8):1419-27. PubMed PMID: 15953606.

77. Riddle RC, Taylor AF, Genetos DC, Donahue HJ. MAP kinase and calcium signaling mediate fluid flow-induced human mesenchymal stem cell proliferation. Am J Physiol Cell Physiol. 2006 Mar;290(3):C776-84. PubMed PMID: 16267109.

78. Li Z, Zhou Z, Saunders MM, Donahue HJ. Modulation of connexin43 alters expression of osteoblastic differentiation markers. Am J Physiol Cell Physiol. 2006 Apr;290(4):C1248-55. PubMed PMID: 16319124.

79. Cher ML, Towler DA, Rafii S, Rowley D, Donahue HJ, Keller E, Herlyn M, Cho EA, Chung LW. Cancer interaction with the bone microenvironment: a workshop of the National Institutes of Health



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Tumor Microenvironment Study Section. Am J Pathol. 2006 May;168(5):1405-12. PubMed PMID: 16651608; PubMed Central PMCID: PMC1606588.

80. Le M, Li Z, Donahue HJ, Chinoy MR. Connexin 43 gene expression in cardio-pulmonary defects in

murine model of congenital diaphragmatic hernia. Front Biosci. 2006;11:3014-25. PubMed PMID: 16720372

81. Taylor AF, Saunders MM, Shingle DL, Cimbala JM, Zhou Z, Donahue HJ. Mechanically

stimulated osteocytes regulate osteoblastic activity via gap junctions. Am J Physiol Cell Physiol. 2007 Jan;292(1):C545-52. PubMed PMID: 16885390.

82. Hansen JC, Lim JY, Xu LC, Siedlecki CA, Mauger DT, Donahue HJ. Effect of surface nanoscale topography on elastic modulus of individual osteoblastic cells as determined by atomic force microscopy. J Biomech. 2007;40(13):2865-71. PubMed PMID: 17467715; PubMed Central PMCID: PMC3607429.

83. Riddle RC, Taylor AF, Rogers JR, Donahue HJ. ATP release mediates fluid flow-induced proliferation of human bone marrow stromal cells. J Bone Miner Res. 2007 Apr;22(4):589-600. PubMed PMID: 17243863.

84. Lim JY, Dreiss AD, Zhou Z, Hansen JC, Siedlecki CA, Hengstebeck RW, Cheng J, Winograd N,

Donahue HJ. The regulation of integrin-mediated osteoblast focal adhesion and focal adhesion kinase expression by nanoscale topography. Biomaterials. 2007 Apr;28(10):1787-97. PubMed PMID: 17218005.

85. Genetos DC, Kephart CJ, Zhang Y, Yellowley CE, Donahue HJ. Oscillating fluid flow activation of gap junction hemichannels induces ATP release from MLO-Y4 osteocytes. J Cell Physiol. 2007 Jul;212(1):207-14. PubMed PMID: 17301958; PubMed Central PMCID: PMC2929812.

86. Lim JY, Donahue HJ. Cell sensing and response to micro- and nanostructured surfaces produced by chemical and topographic patterning. Tissue Eng. 2007 Aug;13(8):1879-91. PubMed PMID: 17583997.

87. Godin LM, Suzuki S, Jacobs CR, Donahue HJ, Donahue SW. Mechanically induced intracellular calcium waves in osteoblasts demonstrate calcium fingerprints in bone cell mechanotransduction. Biomech Model Mechanobiol. 2007 Nov;6(6):391-8. PubMed PMID: 17082961; PubMed Central PMCID: PMC2874246.

88. Liu X, Lim JY, Donahue HJ, Dhurjati R, Mastro AM, Vogler EA. Influence of substratum surface chemistry/energy and topography on the human fetal osteoblastic cell line hFOB 1.19: Phenotypic and genotypic responses observed in vitro. Biomaterials. 2007 Nov;28(31):4535-50. PubMed PMID: 17644175; PubMed Central PMCID: PMC2705827.

89. Huang X, Zhang Y, Donahue HJ, Lowe TL. Porous thermoresponsive-co-biodegradable hydrogels as tissue-engineering scaffolds for 3-dimensional in vitro culture of chondrocytes. Tissue Eng. 2007 Nov;13(11):2645-52. PubMed PMID: 17683245.



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90. Riddle RC, Taylor AF, Donahue HJ. Fluid flow assays. Methods Mol Biol. 2008;455:335-45. PubMed PMID: 18463829.

91. Li Z, Zhou Z, Donahue HJ. Alterations in Cx43 and OB-cadherin affect breast cancer cell metastatic potential. Clin Exp Metastasis. 2008;25(3):265-72. PubMed PMID: 18193170.

92. Li Z, Zhou Z, Welch DR, Donahue HJ. Expressing connexin 43 in breast cancer cells reduces their metastasis to lungs. Clin Exp Metastasis. 2008;25(8):893-901. PubMed PMID: 18839320; PubMed Central PMCID: PMC2754227.

93. Kapoor P, Suva LJ, Welch DR, Donahue HJ. Osteoprotegrin and the bone homing and colonization potential of breast cancer cells. J Cell Biochem. 2008 Jan 1;103(1):30-41. PubMed PMID: 17471510.

94. Lim JY, Shaughnessy MC, Zhou Z, Noh H, Vogler EA, Donahue HJ. Surface energy effects on osteoblast spatial growth and mineralization. Biomaterials. 2008 Apr;29(12):1776-84. PubMed PMID: 18222536.

95. Riddle RC, Hippe KR, Donahue HJ. Chemotransport contributes to the effect of oscillatory fluid flow on human bone marrow stromal cell proliferation. J Orthop Res. 2008 Jul;26(7):918-24. PubMed PMID: 18327808.

96. Ciovacco WA, Goldberg CG, Taylor AF, Lemieux JM, Horowitz MC, Donahue HJ, Kacena MA. The role of gap junctions in megakaryocyte-mediated osteoblast proliferation and differentiation. Bone. 2009 Jan;44(1):80-6. PubMed PMID: 18848655; PubMed Central PMCID: PMC2659565.

97. Jekir MG, Donahue HJ. Gap junctions and osteoblast-like cell gene expression in response to fluid flow. J Biomech Eng. 2009 Jan;131(1):011005. PubMed PMID: 19045921.

98. Riddle RC, Donahue HJ. From streaming-potentials to shear stress: 25 years of bone cell mechanotransduction. J Orthop Res. 2009 Feb;27(2):143-9. PubMed PMID: 18683882.

99. Salvi JD, Lim JY, Donahue HJ. Finite element analyses of fluid flow conditions in cell culture. Tissue Eng Part C Methods. 2010 Aug;16(4):661-70. PubMed PMID: 19778171; PubMed Central PMCID: PMC2945919.

100. Lloyd SA, Donahue HJ. Gap Junctions and Biophysical Regulation of Bone Cells. Clin Rev Bone Miner Metab. 2010 Dec 1;8(4):189-200. PubMed PMID: 23762015; PubMed Central PMCID: PMC3677790.

101. Salvi JD, Lim JY, Donahue HJ. Increased mechanosensitivity of cells cultured on nanotopographies.

J Biomech 2010 Nov 16;43 (15):3058-62. PMID: 20851397; PubMed Central PMCID: PMC3614341

102. Wright TM, Buckwalter JA, Donahue HJ. Plagiarism (editorial). J. Ortho. Res. 2010;28 (9) 1119.

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103. Bridgeman JT, Zhang Y, Donahue HJ, Wade AM, Juliano PJ. Estrogen receptor expression in posterior tibial tendon dysfunction; a pilot study. Foot Ankle Int. 2010;31(12)1081-84. PMID: 21189209

104. Fitzpatrick LR, Green C, Frauenhoffer EE, French KJ, Zhuang Y, Maines LW, Upson JJ, Paul E,

Donahue HJ, Mosher TJ, Smith CD. Attenuation of arthritis in rodents by a novel orally-available inhibitor of sphingosine kinase. Inflammopharmacology, 2011;19:75–87.DOI 10.1007/s10787-010-0060-6

105. Zhang Y, Paul EM, Sathyendra V, Davison A, Sharkey N, Bronson S, Srinivasan S, Gross TS,

Donahue HJ. Enhanced osteoclastic resorption and responsiveness to mechanical load in gap junction deficient bone. PLoS One. 2011;6(8):e23516. PubMed PMID: 21897843; PubMed Central PMCID: PMC3163577.

106. Genetos DC, Karin NJ, Geist DJ, Donahue HJ, Duncan RL. Purinergic signaling is required for fluid shear stress-induced NF-κB translocation in osteoblasts. Exp Cell Res. 2011 Apr 1;317(6):737-44. PubMed PMID: 21237152; PubMed Central PMCID: PMC3049820.

107. Buckwalter JA, Wright TM, Donahue HJ. A new feature of the journal of orthopaedic research: research perspectives. J Orthop Res. 2011 Jun;29(6):801. PubMed PMID: 21520253.

108. Xing Y, Gu Y, Xu LC, Siedlecki CA, Donahue HJ, You J. Effects of membrane cholesterol depletion and GPI-anchored protein reduction on osteoblastic mechanotransduction. J Cell Physiol. 2011 Sep;226(9):2350-9. PubMed PMID: 21660958; PubMed Central PMCID: PMC3071886.

109. Lim JY, Loiselle AE, Lee JS, Zhang Y, Salvi JD, Donahue HJ. Optimizing the osteogenic potential of adult stem cells for skeletal regeneration. J Orthop Res. 2011 Nov;29(11):1627-33. PubMed PMID: 21509820; PubMed Central PMCID: PMC3263698.

110. Lim JY, Siedlecki CA, Donahue HJ. Nanotopographic cell culture substrate: polymer-demixed nanotextured films under cell culture conditions. Biores Open Access. 2012 Oct;1(5):252-5. PubMed PMID: 23515067; PubMed Central PMCID: PMC3559240.

111. Lloyd SA, Lewis GS, Zhang Y, Paul EM, Donahue HJ. Connexin 43 deficiency attenuates loss of trabecular bone and prevents suppression of cortical bone formation during unloading. J Bone Miner Res. 2012 Nov;27(11):2359-72. PubMed PMID: 22714552; PubMed Central PMCID: PMC3683470.

112. Genetos DC, Zhou Z, Li Z, Donahue HJ. Age-related changes in gap junctional intercellular communication in osteoblastic cells. J Orthop Res. 2012 Dec;30(12):1979-84. PubMed PMID: 22696456; PubMed Central PMCID: PMC3640546.

113. Loiselle AE, Paul EM, Lewis GS, Donahue HJ. Osteoblast and osteocyte-specific loss of Connexin43 results in delayed bone formation and healing during murine fracture healing. J Orthop Res. 2013 Jan;31(1):147-54. PubMed PMID: 22718243; PubMed Central PMCID: PMC3640531.


















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114. Loiselle AE, Lloyd SA, Paul EM, Lewis GS, Donahue HJ. Inhibition of GSK-3β rescues the impairments in bone formation and mechanical properties associated with fracture healing in osteoblast selective connexin 43 deficient mice. PLoS One. 2013;8(11):e81399. PubMed PMID: 24260576; PubMed Central PMCID: PMC3832658.

115. Zhang Y, Chin-Quee K, Riddle RC, Li Z, Zhou Z, Donahue HJ. BRMS1 Sensitizes Breast Cancer Cells to ATP-Induced Growth Suppression. Biores Open Access. 2013 Apr;2(2):77-83. PubMed PMID: 23593560; PubMed Central PMCID: PMC3620472.

116. Govey PM, Loiselle AE, Donahue HJ. Biophysical regulation of stem cell differentiation. Curr Osteoporos Rep. 2013 Jun;11(2):83-91. PubMed PMID: 23532405.

117. Loiselle AE, Jiang JX, Donahue HJ. Gap junction and hemichannel functions in osteocytes. Bone. 2013 Jun;54(2):205-12. PubMed PMID: 23069374.

118. Loiselle AE, Wei L, Faryad M, Paul EM, Lewis GS, Gao J, Lakhtakia A, Donahue HJ. Specific biomimetic hydroxyapatite nanotopographies enhance osteoblastic differentiation and bone graft osteointegration. Tissue Eng Part A. 2013 Aug;19(15-16):1704-12. PubMed PMID: 23510012; PubMed Central PMCID: PMC3700012.

119. Donahue HJ, Genetos DC. Genomic approaches in breast cancer research. Brief Funct

Genomics. 2013 Sep;12(5):391-6. PubMed PMID: 23788797; PubMed Central PMCID: PMC3776566.

120. Lloyd SA, Loiselle AE, Zhang Y, Donahue HJ. Connexin 43 deficiency desensitizes bone to the effects of mechanical unloading through modulation of both arms of bone remodeling. Bone. 2013 Nov;57(1):76-83. PubMed PMID: 23891909.

121. Lloyd SA, Lang CH, Zhang Y, Paul EM, Laufenberg LJ, Lewis GS, Donahue HJ. Interdependence of muscle atrophy and bone loss induced by mechanical unloading. J Bone Miner Res. 2014;29(5):1118-30. PubMed PMID: 24127218; PubMed Central PMCID: PMC4074925.

122. Xing Y, Gu Y, Bresnahan JJ, Paul EM, Donahue HJ, You J. The roles of P2Y2 purinergic receptors in osteoblasts and mechanotransduction. PLoS One. 2014;9(9):e108417. PubMed PMID: 25268784; PubMed Central PMCID: PMC4182465.

123. Lloyd SA, Loiselle AE, Zhang Y, Donahue HJ. Shifting paradigms on the role of connexin43 in the skeletal response to mechanical load. J Bone Miner Res. 2014 Feb;29(2):275-86. PubMed PMID: 24588015.

124. Lloyd SA, Loiselle AE, Zhang Y, Donahue HJ. Evidence for the role of connexin 43-mediated intercellular communication in the process of intracortical bone resorption via osteocytic osteolysis. BMC Musculoskelet Disord. 2014 Apr 9;15:122. PubMed PMID: 24716486; PubMed Central PMCID: PMC3984635.

125. Govey PM, Jacobs JM, Tilton SC, Loiselle AE, Zhang Y, Freeman WM, Waters KM, Karin NJ,

Donahue HJ. Integrative transcriptomic and proteomic analysis of osteocytic cells exposed to fluid flow reveals novel mechano-sensitive signaling pathways. J Biomech. 2014 Jun 3;47(8):1838-45. PubMed PMID: 24720889; PubMed Central PMCID: PMC4037855.





















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126. Sathyendra V, Donahue HJ, Vrana KE, Berg A, Fryzel D, Gandhi J, Reid JS. Single Nucleotide Polymorphisms in Osteogenic Genes in Atrophic Delayed Fracture-Healing: A Preliminary Investigation. J Bone Joint Surg Am. 2014 Aug 6;96(15):1242-1248. PubMed PMID: 25100770.

127. Plotkin LI, Speacht TL, Donahue HJ. Cx43 and Mechanotransduction in Bone. Curr Osteoporos

Rep. 2015 Apr;13(2):67-72. PMID: 25616771.

128. Jariwala SH, Lewis GL, Bushman ZJ, Adair JH, Donahue HJ. 3D Printing of Personalized Artificial

Bone Scaffolds. 3D Printing and Additive Manufacturing. 2015 2(2): 56-64.

129. Krause AR, Speach, TA, Ateine, JL, Govey PM, Lang CM, Donahue HJ. Sclerostin Deficiency Attenuates Unloading Induced Bone Loss but has Catabolic Effects on Muscle. J Bone Min Res. 2015. In Preparation.

130. Chin-Quee K, Donahue HJ. MDA-MET-conditioned-medium augments the chemoattractant-dependent migration of MDA-MET cells towards hFOB-conditioned medium and increases collagenase activity. BMC Cancer 2015. In Review.

131. Govey, PM, Kawasawa, YI Donahue HJ. Mechano-sensitive Gene Expression with RNA-Seq:

Revisiting the Osteocytic Cell Response to Fluid Flow. J. Biomech. 2015 Dec 16;48(16):4327-32 PMID: 26573903.

132. Andalib MN, Dzenis, Y, Donahue, HJ, Lim, JY. Biomimetic substrate control of cellular

mechanotransduction. Biomater Res. 2016 Apr 29;20:11. 2016. PMID: 27134756

133. Jariwala SH, Wee H, Roush EP, Whitcomb TL, Murter C, Kozlansky G, Lakhtakia A, Kunselman

AR, Donahue HJ, Armstrong AD, Lewis, GS. Time Course of Peri-Implant Bone Regeneration around Loaded and Unloaded Implants in a Rat Model. J Orthop Res. 2016 Jul 6. [Epub ahead of print] PMID: 2738180.

134. Govey PM, Zhang Y, Donahue HJ. Mechanical loading attenuates radiation-induced bone loss in bone marrow transplanted mice. PLoS One. December 9, 2016, http://dx.doi.org/10.1371/journal.pone.0167673.

135. Mellor LF, Trevisan B, Taylor MA, Krause AR, Donahue HJ, Oxford JT, Loboa EG. Sclerostin inhibition in microgravity and reduced loading may be detrimental for articular cartilage health. Bone. In Review.

Book Chapters:

1. McLeod, K.J., Donahue, H.J., Levin, P.E. and Rubin, C.T. (1991) Low frequency sinusoidal electric fields alter calcium fluctuations in osteoblast-like cells. In: Electromagnetics in Biology and Medicine (Brighton, C.T. and S.R. Pollack, ed.), San Francisco Press, pp. 111-115.

2. Donahue, H.J. (1992) Regulation of signal transduction in osteoclasts. In: The Biology and Physiology of the Osteoclast (Rifkin, B.R. and C.V. Gay, ed.), CRC Press, Inc. pp. 207-222.


http://www.ncbi.nlm.nih.gov/pubmed?term=%22Current+osteoporosis+reports%22%5BJour%5D+AND+2015%5Bpdat%5D+AND+Plotkin%5Bauthor%5D&cmd=detailssearch

http://dx.doi.org/10.1371/journal.pone.0167673

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3. Rubin, C.T., McLeod, K.J., Gross, T.S. and Donahue, H.J. (1992) Physical stimuli as potent determinants of bone morphology. In: Bone Biodynamics in Orthodontic and Orthopaedic Treatment (D.S. Carlson and S.A. Goldstein, ed.), The University of Michigan, pp. 75-91.

4. McLeod, K.J., Donahue, H.J., Fontaine, M.A. and Rubin, C.T. (1992) Cell density effects on the interaction of electric fields with cells in vitro. In: Electromagnetics in Biology and Medicine (Martin Blank, ed.) San Francisco Press, pp. 291-294.

5. McLeod, K.J., Rubin, C.T., Donahue, H.J., and Guilak, F. (1992) The role of polarization forces in mediating the interaction of low frequency electric fields with living tissue. In: Electromagnetics in Biology and Medicine (Martin Blank, ed.) San Francisco Press, pp. 10-15.

6. Guilak, F., Donahue, H.J., Zell, R.A., Luo, S., Grande, D., McLeod, K.J. and Rubin, C.T. (1994) Deformation-induced calcium signalling in articular chondrocytes. In Cell Mechanics and Cellular Engineering (Mow V., Guilak F., Hochmutch R., and Transontay R., eds.) Springer-Verlag, NY, pp.380-397.

7. Rubin, C.T., Gross, T., Donahue, H.J., Guilak F. and McLeod K.J. (1995) Physical and environmental influences on bone formation. In: Bone Formation and Regeneration, AAOS, pp. 61-78.

8. Donahue, H.J., Chen, Q., Jacobs, C.R., Saunders, M.M and Yellowley, C.E. (2003) Bone cells and mechanotransduction. In Orthopaedics and Molecular Biology, (Rosier R.N. and Evans C.E. eds.) American Academy of Orthopaedic Surgeons. Rosemont, IL. pp 179-190.

9. Donahue, H.J., Siedlecki, C.A., Vogler, E. (2004) Osteoblastic and Osteocytic Biology and Bone Tissue Engineering. In Bone Tissue Engineering, (Hollinger J.O., Einhorn T.A., Doll B.A. and Sfeir, C. eds.) CRC Press, Inc, pp 44-54.

10. Riddle, R.C., Taylor, A.F. and Donahue, H.J. (2008) Fluid flow assays. In Osteoporosis-Methods in Molecular Medicine 455:335-45 (Westendorf, J.J. eds.) Humana Press.

Abstracts:

1. Donahue, H.J., Penniston, J.T. and Heath III, H. (1987) Can erythrocyte (RBC) membrane calcium-ATPase (Ca-ATPase) activity be measured reproducibly for studies of human disease? J. Bone Min. Res. 2(S1) 99.

2. Eriksen, E.F., Fryer, M., Donahue, H.J. and Heath III, H. (1987) Dose-dependent biphasic effect of parathyroid hormone (PTH) on cytosolic calcium in aequorin-loaded ROS 17/2.8 cells. Am. Fed. Clin. Res.

3. Donahue, H.J., Fryer, M., Eriksen, E. and Heath III, H. (1988) Differential effects of parathyroid hormone (PTH) and its analogues on cytosolic calcium ion ([Ca++]i) and cAMP accumulation in ROS 17/2.8 cells. Internatl. Symposium on Clin. Disorders of Bone & Min. Metabol.

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4. Donahue, H.J., Fryer, M. and Heath III, H. (1988) Synthetic fragment 1-37 of human parathyroid hormone-related peptide increases cytosolic ionized calcium (Ca++) in ROS 17/2.8 cells. J. Bone Min. Res. 3(S1) 157.

5. Donahue, H.J., Penniston, J.T. and Heath III, H. (1988) Erythrocyte (RBC) membrane (Ca2+Mg2+)-ATPase kinetics in familial benign hypercalcemia (FBH): A clue to pathogenesis? J. Bone Min. Res. 3(S1) 91.

6. Donahue, H.J., Borke, J.L., Penniston, J.T. and Kumar, R. (1988) Effect of purified 28kDa calcium binding protein (CaBP) on calmodulin-stimulated human erythrocyte membrane (Ca2++Mg2++) -ATPase kinetics. J. Bone Min. Res. 3(S1) 51.

7. Donahue, H.J., Fryer, M. and Heath III, H. (1989) Parathyroid hormone-related peptide (PRP) analogs: Structure-function relationships for effects on cytosolic calcium and cAMP in osteoblastic cells (ROS 17/2.8). Endocrine Soc. 71st Annual Meeting, Seattle, WA.

8. Bidwell, J.P., Barta, J., Donahue, H.J., Firek, A., Fryer, M., Laakso, K.J. and Heath III, H. (1989) Cytosolic ionized calcium (Cai) response to parathyroid hormone (PTH) is preserved in desensitized bone cells. J. Bone Min. Res. 4(S1):342.

9. McLeod, K.J., Donahue, H.J. and Rubin, C.T. (1990) Low frequency electric fields modulate calcium spiking in osteoblast-like cells. J. Bone Min. Res. 5:(S2)17.

10. Donahue, H.J., Iijima, K., Goligorsky, M., Rubin, C. and Rifkin, B. (1990) Tetracycline alters the regulation of cytoplasmic calcium ion in rat osteoclasts. J. Bone Min. Res. 5:(S2)17.

11. McLeod, K.J., Donahue, H.J., Levin, P.E. and Rubin, C.T. (1990) Low frequency sinusoidal electric fields alter calcium fluctuations in osteoblast-like cells. Proc. of the 10th Meeting of the Biol. Repair and Growth Soc., Philadelphia, PA.

12. Donahue, H.J., Iijima, K., Goligorsky, M., Rubin, C.T. and Rifkin, B. (1991) Altered regulation of cytoplasmic calcium ion concentration in tetracycline treated osteoclasts. Tans. Ortho. Res. Soc. 16(2):404.

13. McLeod, K.J., Donahue, H.J. and Rubin, C.T. (1991) Temporal response of bone cells to physical stimuli: Changes in calcium mobilization following electrical stimulation. Trans. Ortho. Res. Soc. 16(1):12.

14. Rifkin, B., Donahue, H.J., Iijima, K., Goligorsky, M. and Rubin, C.T. (1991) Tetracyclines attenuate extracellular calcium-stimulated cytosolic calcium mobilization in osteoclasts. J. Dental Res. 70:495.

15. Levin, P.E., McLeod, K.J., Donahue, H.J. and Rubin, C.T. (1991) Identification of the biologically active component of ultrasound: Signal isolation and analysis for optimization stimulation. Proc. 6th German-Austrian-Swiss Trauma Congress, Vienna, Austria.

16. Donahue, H.J., and Brink, P.R. (1991) T-type calcium currents in osteoblast-like cells: Electrophysiological evidence for regulation by PTH. Endocrine Soc. 73rd Annual Meeting, Washington, D.C.

25

17. Donahue, H.J. and Brink, P.R. (1991) Regulation of calcium channels in ROS 17/2.8 cells: PTH activates T-type calcium currents. In: Molecular Basis of Bone Cell Physiology: Signaling, St. Louis, MO.

18. Donahue, H.J., McLeod, K.J., Levin, P.E., Rubin, C.T. and Brink, P.R. (1991) Correlation of calcium spiking and T-type calcium currents in osteoblast-like cells. J. Bone Min. Res. 6(S1)52.

19. Donahue, H.J., McLeod, K.J., Zapatero, J., Levin, P.E., Rubin, C.T. and Brink, P.R. (1992) Cellular basis for the pleiotropic effect of PTH on bone metabolism: Effect of PTH on cytosolic Ca2++ dynamics in osteoblasts. Trans. Ortho. Res. Soc. 17(1):180.

20. Donahue, H.J., and Rubin, C.T. (1992) Age-induced disruption of signal transduction in osteoblasts: Desensitization to parathyroid hormone. Bone & Mineral 17(S1):123.

21. McLeod, K.J., Donahue, H.J., Fontaine, M.A. and Rubin, C. T. (1992) Cell density effects on the interaction of electric fields with cells in vitro. First World Congress for Electricity and Magnetism in Biology and Medicine, pp. 11, Lake Buena, Vista, Florida.

22. McLeod, K.J., Rubin, C.T., Donahue, H.J. and Guilak, F. (1992) The role of polarization forces in mediating the interaction of low frequency electric fields with living tissue. First World Congress for Electricity and Magnetism in Biology and Medicine, pp. 155, Lake Buena Vista, Florida.

23. Donahue, H.J., Grine, E.A., McLeod, K.J., Rubin, C.T., Hertaberg, E.L. and Brink, P.R. (1992) Distribution and hormonal regulation of gap junctional proteins in bone cell networks. Endocrine Society, 74th Annual Meeting, San Antonio, TX.

24. McLeod, K.J., Rubin, C.T., Donahue, H.J. and Guilak, F. (1992) On the mechanism of extremely low frequency (ELF) electric field interactions with living tissue. IEEE North East Biomedical Engineering 18:65-66.

25. McLeod, K.J., Rubin, C.T., Donahue, H.J., Guilak, F. and Mase, C.A. (1992) Characterization of the extremely low frequency (ELF) electric field interactions with living tissue. International Conference on Engineering in Medicine and Biology, Paris, France.

26. Donahue, H.J., Porres, L., Dee, C. and Rubin, C. T. (1992) Age-induced desensitization to parathyroid hormone in osteoblasts: A defect in adenylate cyclase is not involved. J. Bone Min. Res. 7(S1):S216.

27. Donahue, H.J., Grine, E.A., Andersen, A.J., McLeod, K.J., Rubin, C.T., Hertzberg, E.L. and Brink, P.R. (1992) Distribution, expression and hormonal regulation of gap junctional proteins in bone cell networks. J. Bone Min. Res. 7(S1): S127.

28. McLeod, K.J., Donahue, H.J., Levin, P.E., Fontaine, M. and Rubin, C.T. (1992) Electric fields modulate bone cell function in a density-dependent manner. J. Bone Min. Res. 7(S1): S216.

29. McLeod, K.J, Donahue, H.J., Fontaine, M.A., Rubin, C.T. (1993) Osteogenic electric fields modulate bone cell function in vitro in a cell density-dependent manner. Trans. Ortho. Res. Society 18:491.

26

30. Donahue, H.J., McLeod, K.J., Mase, C.A., Rubin, C.T., Andersen, J.A., Grine, E.A., Hertzberg, E.L. and Brink, P.R. (1993) Cell-to-cell communication in osteoblastic cell networks: Distribution, expression and hormonal regulation of connexin 43. Trans. Ortho. Res.Society 18:73.

31. Donahue, H.J., McLeod, K.J., Rubin, C. T., Fishman, G.I. and Brink, P.R. (1993) Age-induced attenuation of intercellular communication in osteoblastic cells. J. Bone Min. Res. 8(S1): 125.

32. Rubin, C.T., Gross, T.S., Donahue, H. J., Guilak, F. and McLeod, K.J. (1993) Promotion of bone formation by frequency specific, low amplitude biophysical stimuli. International Society for Fracture Repair, Ulm, Germany.

33. Donahue, H.J., McLeod, K.J., Rubin, C.T., Hertzberg, E.L., Brink, P.R. (1993) Age-induced attenuation of hormonally regulated intercellular communication in bone cell networks. International Meeting on Gap Junctions, Hiroshima, Japan.

34. Donahue, H.J., Guilak, F., McLeod, K.J., Rubin, C.T., Hertzberg, E.L. and Brink, P.R. (1993) Mechano-chemical signal transduction in chondrocytes: A role for gap junctions? 33rd Annual Meeting of the American Society for Cell Biology, New Orleans, LA.

35. McLeod, K.J., Donahue, H.J. and Rubin, C.T. (1993) Ca2+ Transients during mitotic progression in ensembles of osteoblastic cells: A basis for monitoring cellular responses to ELF in real time. 1993 Annual Review of Research on Biological Effects of Electric and Magnetic Fields from the Generation, Delivery and Use of Electricity.

36. Donahue, H.J., Guilak, F., Grande, D., Bibb, M., Porres, L., McLeod, K., Rubin, C., Grine, E., Hertzberg, E. and Brink, P. (1994) Intercellular communication via gap junctions in chondrocytes isolated from mature articular cartilage. Trans. Ortho. Res. Society 19:375.

37. Rubin, C., Gross, T., Guilak, F., Donahue, H.J. and McLeod, K. (1994) Differentiation of the intracortical remodeling response to axial and torsional loading. Trans. Ortho. Res. Society 19:34.

38. Guilak, F., Donahue, H.J., McLeod, K.J., Grande, D., Porres, L. and Rubin, C.T. (1994) Intracellular and intercellular propagation of deformation-induced calcium transients in articular chondrocytes. Trans. Ortho. Res. Society 19:259.

39. McLeod, K.J., and Donahue, H.J. (1994) Ca2+ transients as a transduction signal for extremely low frequency electric fields. Annual Meeting of the Biophysical Society New Orleans, LA.

40. Donahue, H.J., and McLeod, K.J. (1994) Spontaneous Ca2+ transients recorded fromosteoblastic cells: A real-time measurement of cellular responses to ELF fields. FASEB Journal 8:5.

41. Guilak, F., Donahue, H.J., Zell, R.A., Luo, S., Grande, D., McLeod, K.J. and Rubin, C.T. (1994) Deformation-induced calcium signaling in articular chondrocytes. Proceeding of the second World Congress of Biomechanics, eds.

27

42. Donahue, H.J., Guilak, F., Zell, R.A,. Luo, S., Vander Molen, M., Sun, Y., Rubin, C.T. Grande, D. and Brink, P.R. (1994) Intercellular communication and mechano-chemical signal transduction in chondrocytes. Bone and Mineral 25(52)116.

43. Donahue, H.J., Porress, L. and Rubin, C.T. (1994) PTH regulation of inter- and intracellular communication in bone cell networks attenuates with age. Third International Meeting on New Actions of Parathyroid Hormone, Kyoto, Japan.

44. Vander Molen, M.A., Rubin, C.T., Brink, P.R. and Donahue, H.J. (1994) Characterization of a novel intercellular communication deficient bone cell line. J. Bone Min. Res. 9(S1)S199.

45. Guilak, F., Donahue, H.J., Zell, R.A., Grande, D., McLeod, K.J. and Rubin, C.T. (1994) Deterioration of mechanical signal transduction in osteoarthritic cartilage. The Whitaker Conference.

46. Guilak, F., Donahue, H.J., McLeod, K.J., Grande, D.A., Zell, R.A. and Rubin, C.T. (1994) Gadolinium and amiloride attenuate mechanically-induced calcium wave propagation in articular chondrocytes. Proceedings of the Society for Physical Regulation in Biology and Medicine.

47. McLeod, K.J., Porres, L. and Donahue, H.J. (1994) Electric fields induce changes in calcium ion activity response in osteoblastic cells: frequency and dose dependence. Proceedings of the Society for Physical Regulation in Biology and Medicine.

48. Vander Molen, M.A., Rubin, C.T., and Donahue, H.J. (1995) Decreased hormonal responsiveness in gap junction deficient osteoblasts. Trans. Ortho. Res. Society 20(2)295.

49. Donahue, H.J., Rubin, C.T. and Vander Molen, M.A. (1995) Hormonal regulation of cAMP in gap junction deficient osteoblastic cells. XIIth International Conference on Calcium Regulating Hormones, Melbourne, Australia.

50. Donahue, H.J., Westerlind, K.C., Turner, R.T. and Vander Molen, M.A. (1995) Skeletal unloading increases steady state levels of connexin 43 mRNA. FASEB Journal 9(4) A673.

51. Donahue, H.J., Westerlind, K.C., Li, Z., Turner, R.T., Morey-Holton, E. and Vander Molen, M.A. (1995) Connexin 43 mRNA is reversibly upregulated by skeletal unloading: a role for gap junctional communication in biophysical signal amplification? Trans. Second Meet. Combined Ortho. Res. Soc. US, Japan, Canada and Europe.

52. Donahue, H.J., Rubin, C.T. and Vander Molen, M.A. (1995) Decreased hormonal responsiveness in gap junction deficient osteoblastic cells. Annual Meeting of the Endocrine Society.

53. Donahue, H.J., Li, Z., Zhou, Z. and Simon, B. (1995) Pulsed electromagnetic fields affect steady state levels of Cx43 mRNA in osteoblastic cells. J. Bone Min. Res. 10(S1)s207.

54. Li, Z., Zhou, Z. and Donahue, H.J. (1995) Differential expression of Cx43 in osteoblastic cell lines. J. Bone Min. Res. 10(S1)s207.

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55. Vander Molen, M.A., McLeod, K.J., Donahue, H.J. and Rubin, C.T. (1996) The influence of intercellular communication in defining osteoblast activity to biophysical stimuli. Trans. Ortho. Res. Soc. 2(2)338.

56. Guilak, F., Setton, L.A. and Donahue, H.J. (1996) Cartilage response to mechanical stress. In: Proceedings of the Keystone Symposia on Tissue Engineering and Wound Repair in Context, Taos, New Mexico. p.8.J

57. Yellowley, C.E., Jacobs, C.R., Zhou, Z. and Donahue, H.J. (1996) Pulsatile fluid flow-induced shear stress increases intracellular calcium concentration in bovine articular chondrocytes. Biophysical Journal 70(2)2:A365.

58. Donahue, H.J., Li, Z., Zhou, Z. and McCauley, L.K. (1996) Age-related decreases in stimulatory G-protein coupled adenylate cyclase activity in osteoblastic cells. J. Bone Min. Res., 11(S1)S384.

59. Yellowley, C.E., Jacobs, C.R., Zhou, Z., Paul, E.M. and Donahue, H.J. (1996) Pulsatile fluid flow affects cytosolic calcium dynamics in bovine articular chondrocytes. J. Bone Min. Res., 11(S1)S298.

60. Li, Z., Zhou, Z., Conway, J.L. and Donahue, H.J. (1996) Cyclic strain decreases osteocalcin mRNA expression in osteoblastic cells. J. Bone Min. Res., 11(S1)S263.

61. Lee, Y.C., Yellowley, C.E., Li, Z., Donahue, H.J. and Rannels, D.E. (1996) Gap junction mediated epithelial cell interactions following exposure to anthracite coal dust in vitro. 9th International Colloquium on Pulmonary Fibrosis.

62. Li, Z., Zhou, Z. and Donahue, H.J. (1997) Cx43 expression parallels human osteoblastic cell differentiation. Trans. Ortho. Res. Soc., 22(52)593.

63. Donahue, H.J., Bowman, N. and Ehrlich, H.P. (1997) Gap junctions contribute to collagen lattice contraction by osteoblastic cells. FASEB Journal 11(3)2602.

64. Lee, Y.C., Li, Z., Donahue, H.J. and Rannels, D.E. (1997) Differential expression of connexin mRNAs in lungs of rat, mouse and dog and isolated rat type II cells. Am J Resp Crit. Care Med. 155: A873, 1997.

65. Donahue, H.J., Bowman, N. and Ehrlich, H.P. (1997) Osteoblastic cell organization of extracellular matrix is dependent on gap junctions. J. Bone Min. Res.12(S1)S200.

66. Donahue, H.J., Yellowley, C.E., Jacobs, C.R., Zhou, Z. and Li, Z. (1997) Gap junctions and fluid flow-induced shear stress. Annals of Biomedical Engineering 25(S1)S76.

67. Donahue, H.J., Bowman, N. and Ehrlich, H.P. (1997) Gap junctions and collagen gel lattice contraction. Annals of Biomedical Engineering 25(S1)S82.

68. Yellowley, C.E., Jacobs, C.R. and Donahue, H.J. (1997) Ca2+ pools and fluid flow-induced Ca2+

oscillations. Annals of Biomedical Engineering 25(S1)S82.

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69. Jacobs, C.R., Yellowley, C.E. and Donahue, H.J. (1997) Differential effect of steady vs oscillating flow on bone cells. Annals of Biomedical Engineering 25(S1)S81.

70. Li, Z., Zhou, Z., Yellowley, C.E. and Donahue, H.J. (1998) Modulation of cell-to-cell communication alters osteocalcin gene expression and alkaline phosphatae activity in osteoblastic cells. Trans. Ortho. Res. Soc., 23(S1)39.

71. Yellowley, C.E., Li, Z., Zhou, Z., Jacobs, C.R. and Donahue, H.J. (1998) Gap junctional communication modulates the Ca2+ response to fluid-flow in osteoblastic cells. Trans. Ortho. Res. Soc., 23(S2)577.

72. Yellowley, C.E., Jacobs, C.R. and Donahue, H.J. (1998) Intracellular Ca2+ oscillations in articular chondrocytes. Trans. Ortho. Res. Soc., 23(S2)185.

73. Jacobs, C.R., Yellowley, C.E., Davis, B.R., Zhou, Z. and Donahue, H.J. (1998) Differential effect of steady versus oscillating flow on bone cells. Trans. Ortho. Res. Soc., 23(S2)543.

74. Donahue, H.J., Li, Z., Zhou, Z. and Yellowley, C.E. (1998) Gap junctions and human osteoblast differentiation. International Conference on Progress in Bone and Mineral Research. Bone 22 (4) 415.

75. Jacobs, C.R., Yellowley, C.E., Davis, B.R., Zhou, Z. and Donahue, H.J. (1998) Differential effect of steady versus oscillating flow on bone cells. J. Biomech., 31(S1):55.

76. Jacobs, C.R., Yellowley, C.E., Davis, B.R., Zhou, Z., Cimbala, J.M. and Donahue, H.J. (1998) Steady versus oscillating fluid flow in bone cell mechanotransduction. Bone 23 (5) S540.

77. Buhl, K., Jacobs, C.R., Turner, R.T., Evans, G. and Donahue, H.J. (1998) Parallel changes in extracellular matrix protein gene expression, bone formation and biomechanical properties in aging rat bone. Bone 23 (5) S300.

78. Rannels, D.E., Yellowley, C.E., Donahue, H.J. and Lee, Y.C. (1998) The sequelae of coal dust exposure suggest a role for cell-cell interactions in the response of alveolar epithelial cells to injury. The Aspen Lung Conference.

79. Jacobs, C.R., Yellowley, C.E., Davis, B.R., Zhou, Z. and Donahue, H.J. (1998) Differential effect of steady versus oscillating flow on bone cells. 11th Conference of the European Society of Biomechanics, Toulouse.

80. Buhl, K.M., Jacobs, C.R., Turner, R.T., Evans, G.L. and Donahue, H.J. (1999) Changes in extracellular matrix protein gene expression and bone formation parallel decreased biomechanical properties in aging rat bone. Trans of Orthop Res Soc, 24:559.

81. Edlich, M.J., Jacobs, C.R., McIlmoyle, E.L., Yellowley, C.E. and Donahue, H.J. (1999) Effect of steady and oscillating flow on intracellular calcium dynamics in bovine articular chondrocytes. Trans of Orthop Res Soc, 24:650.

82. You, J., Yellowley, C.E., Donahue, H.J. and Jacobs, C.R. (1999) Bone cells do not respond with increased calcium to physiologic levels of substrate deformation. Trans of Orthop Res Soc,

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24:547.

83. Rannels, D.E., Guo,Y., Donahue, H.J., Yellowley, C.E., Martinez-Williams, C., Gilbert, K.A. and Lee, Y-C (1999) Gap junction intercellular communication between pulmonary alveolar epithelial cells. BED-Vol. 42, p433 Bioengineering Conference.

84. Yellowley, C.E. and Donahue, H.J. (1999) Swelling sensitive ion channel currents in bovine articular chondrocytes. XXVIth European Symposium on Calcified Tissues, Maastricht, Calcified Tissue Int. 64(S1):P-243.

85. Donahue, H.J., Li, Z., Zhou, Z., Edlich, M.J., Jacobs, C.R. and Yellowley, C.E. (1999) Gap junctions and mechanotransduction in osteocytes. XXVIth European Symposium on Calcified Tissues, Maastricht, Calcified Tissue Int. 64(S1):P-244.

86. You, J., Yellowley, C.E., Donahue, H.J. and Jacobs, C.R. (1999) Physiological Levels of Substrate Deformation are Less Stimulatory to Bone Cells Compared to Fluid Flow, Proceeding of the 1999 ASME International mechanical Engineering Congress and Exposition, BED-Vol.43: p161-162.

87. Yellowley, C.E. and Donahue, H.J. (2000) Hypotonic swelling increases internal calcium and activates membrane ion channel currents in bovine articular chondrocytes. 46th Annual Meeting of the Orthopaedic Research Society.

88. Saunders, M.M., You, J., Yellowley, C.E., Jacobs, C.R. and Donahue, H.J. (2000) Differential effect of oscillating flow on cytosolic calcium and prostaglandin in osteoblastic ROS 17/2.8 cells. 46th Annual Meeting of the Orthopaedic Research Society.

89. Edlich, M., Yellowley, C.E., Jacobs, C.R. and Donahue, H.J. (2000) Effect of oscillating flow on osteocytic cells is dependent on gap junctional intercellular communication. 46th Annual Meeting of the Orthopaedic Research Society.

90. Edlich, M., Yellowley, C.E., Jacobs, C.R. and Donahue, H.J. (2000) Effect of fluid flow on cycle number and waveform on intracellular calcium concentration in chondrocytes. 46th Annual Meeting of the Orthopaedic Research Society.

91. You, J, Yellowley, C.E., Donahue, H.J. and Jacobs, C.R. (2000) Fluid flow induced calcium mobilization is frequency dependent. 46th Annual Meeting of the Orthopaedic Research Society.

92. You, J., Zhen, X., Yellowley, C.E., Chen, Q., Donahue, H.J. and Jacobs, C.R. (2000) Mechanotransduction in bone cells via oscillating flow. 46th Annual Meeting of the Orthopaedic Research Society.

93. Rumi, M.N., Buhl, K.M., DeMarco, C., Frauenhoffer, E.E., Donahue, H.J., and Black, K.P. (2000) Intermittent high intensity loading exercise damages articular cartilage in the knees of young rats. 46th Annual Meeting of the Orthopaedic Research Society.

94. Donahue, H.J., Li, Z. and Zhou, Z. (2000) Parathyroid hormone regulation of gap junction expression and function in osteoblastic cells from young, mature and old rats. 46th Annual Meeting of the Orthopaedic Research Society.

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95. Kurokouchi, K. and Donahue, H.J. (2000) Oscillating fluid flow decreases TNF- induced NF-B activation in osteoblastic cells. 46th Annual Meeting of the Orthopaedic Research Society.

96. Buhl, K.M., Jacobs, C.R., Evan, G.L., Turner, R.T., Farrell, P.A. and Donahue, H.J. (2000) Aged bone displays an increased responsiveness to low-intensity resistance exercise. 46th Annual Meeting of the Orthopaedic Research Society.

97. Saunders, M.M., Seraj, M.J., Yellowley, C.E., Hoke, A., Welch, D.R., and Donahue, H.J. (2000) Gap junctional intercellular communication is restored in metastasis suppressed breast carcinoma cells. Experimental Biology 2000.

98. Yellowley, C.E., Jacobs, C.R., Donahue, H.J. (2000) Cell swelling induced activation of membrane currents and intracellular calcium in bovine articular chondrocytes. Society for Physical Regulation in Biology and Medicine.

99. Yellowley, C.E., Jacobs, C.R., Li, Z., Donahue, H.J. (2000) Gap junctional intercellular communication in osteocytes. 44th Annual Meeting of the Biophysical Society.

100. Black, K.P., Donahue H.J., Buhl, K. (2000) Intermittent high intensity loading exercise damages articular cartilage in the knees of young rats. 67th Annual Meeting American Academy of Orthopaedic Surgeons.

101. Edlich, M., Yellowley, C.E., Jacobs, C.R., Donahue H.J. (2000) Role of gap junctional intercellular communication on the effects of oscillating fluid flow on osteocytic cells. EORS

2000, 10th Conference of the European Orthopaedic Research Society.

102. Saunders, M.M., Seraj, M.J., Yellowley, C.E., Hoke, A., Welch, D.R., Donahue, H.J. (2000) Gap junctional intercellular communication is restored in metastasis-suppressed breast carcinoma cells. Experimental Biology.

103. Buhl, K.M., Jacobs, C.R., Evans, G.L., Turner, R.T., Farrell, P.A., Donahue, H.J. (2000) Aged bone displays an increased responsiveness to low-intensity exercise. 47th Annual Meeting of the American College of Sports Medicine.

104. You, J., Saunders, M.M., Yellowley, C.E., Donahue, H.J. and Jacobs, C.R. (2000) Frequency dependent effects of oscillating fluid flow on bone cells, Annals of Biomedical Engineering, Trans. BMES Annu. Meet. -- Abstract Supplement 1 (Trans. BMES Annu. Meet.), Vol. 28, S-105.

105. Saunders, M.M., You, J., Trosko, H.Y., Donahue, H.J. and Jacobs, C.R. (2000) Prostaglandin E2 Response in MC3T3 osteoblastic cells is dependent upon gap junction, Annals of Biomedical Engineering, Trans. BMES Annu. Meet. -- Abstract Supplement 1 (Trans. BMES Annu. Meet.), Vol. 28, S-86.

106. Saunders, M.M., You, J., Yellowley, C.E., Donahue, H.J. and Jacobs, C.R. (2000) Prostaglandin E2 response to fluid flow is independent of intracellular calcium concentration in osteoblastic ROS 17/2.8 and RCx16 cells , Annals of Biomedical Engineering, Trans. BMES Annu. Meet. -- Abstract Supplement 1 (Trans. BMES Annu. Meet.) Vol. 28, S-87.

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107. Edlich, M., Yellowley, C.E., Jacobs, C.R. and Donahue, H.J. (2000) Cycle number and waveform of fluid flow affect bovine articular chondrocytes. Proceedings of the European Society of Biomechanics, p 208.

108. Reilly, G.C., Yellowley, C.E., Donahue, H.J. and Jacobs, C.R. (2000) Investigation of the signalling pathways involved in the calcium response of bone cells to oscillating fluid flow. Journal of Bone and Mineral Research p S508.

109. Seraj, M.J., Samant, R.S., Shevde, L.A., Saunders, M.M., Sakamaki, T., Meehan, W.J.,

Donahue, H.J., Debies, M.T., Budgeon, L., Leonard, T.O., Harms, J.F., Christensen, N.D., Winter, C.R., Verderame, M.F., and Welch, D.R. (2000) BRMS1- a human breast cancer metastasis suppressor gene encoded on chromosome 11q13.1-q13.2. Era of Hope- Department of Defense Breast Cancer Research Program Meeting 1: 111-112.

110. Saunders, M.M., Li, Z., Winter, C.R., Welch, D.R. and Donahue, H.J. (2000) Metastatic breast carcinoma correlates with a breakdown in gap junction function and expression. Clinical and Experimental Metastasis (1999) 17: P50. (actual submission and publication 2000).

111. You, J., Saunders, M.M., Yellowley, C.E., Donahue, H.J. and Jacobs, C.R. (2001) Oscillatory flow stimulates PGE2 release via protein kinase A in MC3T3-E1 osteoblasts involving cyclooxygenase-2. 47th Annual Meeting, Transactions of the Orthopaedic Research Society, Vol.26: p326.

112. Saunders, M.M., You, J., Trosko, J., Yamasaki, H., Donahue, H.J. and Jacobs, C.R. (2001) Oscillatory fluid flow-induced prostaglandin E2 production is dependent upon gap junctional intercellular communication in osteoblastic MC3T3-E1 cells. 47th Annual Meeting, Transaction of the Orthopaedic Research Society, Vol.26: p565.

113. Saunders, M.M., Kunze, E., Li, Z., Mastro, A., and Donahue H.J. (2001) Quantification and characterization of gap junctions and gap junctional intercellular communication in an in vitro breast carcinoma model metastatic to bone. 47th Annual Meeting, Transaction of the Orthopaedic Research Society, Vol.26.

114. Li, Z., Zhou, Z. and Donahue, H.J. (2001) Stable transfection of Cx43 antisense inhibits differentiation of human fetal osteoblastic cells. 47th Annual Meeting of the Orthopaedic Research Society.

115. You, J., Saunders, M.M., Yellowley, C.E., Donahue, H.J., and Jacobs, C.R. (2001) Oscillatory flow-induced prostaglandin E2 release involves protein kinase a and cyclooxygenase-2 in MC3T3-E1 osteoblasts, Proceeding of the 2001 ASME Summer Bioengineering Conference, BED-Vol.50: p163-164.

116. Jacobs, C.R., You, J., Reilly, G.C., Saunders, M.M., Kurokouchi, K., Yellowley, C.E. and

Donahue, H.J. (2001) An overview of oscillatory fluid flow as a potent loading-induced physical signal in bone, Proceeding of the 2001 ASME Summer Bioengineering Conference, BED-Vol.50: p339-340.

117. You, J., Saunders, M.M., Yellowley, C.E., Donahue, H.J. and Jacobs, C.R. (2001) Oscillatory flow-induced PGE2 release involves protein kinase A and cyclooxygenase2 in MC3T3-E1

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osteoblasts. SPRBM 20th Annual Meeting, Charleston, SC.

118. Li, Z., Zhou, Z., Saunders, M.M., Casey, G., Welch, D.R., Donahue, H.J. (2001) Connexin and osteopontin expression correlate with breast cancer metastatic potential. Proceedings of the American Association for Cancer Research 42:4244.

119. Haut, T., Donahue, H.J., Jacobs, C. and Yellowley, C.E. (2001) A role for annexin V in bone cell mechanotransduction. Annals of Biomedical Engineering 29(S1)S19.

120. Alford, A.I., Jacobs, C.R. and Donahue, H.J. (2001) Oscillating fluid flow modulates gap junction communication in the osteocyte-like cell line MLOy-4. Annals of Biomedical Engineering 29(S1)S19.

121. You, J., Jacobs, C.R., Donahue, H.J. (2001) Oscillatory fluid flow induces calciummobilization by ATP/UTP via P2Y receptors in osteoblasts. Annals of Biomedical Engineering 29(S1)S25.

122. Alford, A.I., Yellowley, C.E., Jacobs, C.R. and Donahue, H.J. (2001) Regulation of aggrecan gene expression by oscillating fluid flow and intracellular calcium. Annals of Biomedical Engineering 29(S1)S43.

123. Donahue, S., Donahue, H.J. and Jacobs, C.R. (2001) Refractory period for fluid flow induced intracellular calcium oscillations in osteoblastic cells. Annals of Biomedical Engineering 29(S1)S28.

124. Le, M., Dick, A.A., Li, Z., Donahue, H.J. and Chinoy, M.R. (2001) Significance of upregulated connexin 43 expression in cardiopulmonary defects in murine model of congenital diaphragmatic hernia. American Thoracic Society International Meeting.

125. Haut-Donahue, T.L, Donahue, H.J., Jacobs, C.R. and Yellowley, C.E. (2001) A role for annexin V in bone cell mechanotransduction. J. Bone Min. Res. 16 (S1): M191.

126. Alford, A.I., Jacobs, C.R. and Donahue, H.J. (2001) Regulation of Cx43 phosphorylation and gap junction communication in osteocytic MLOY-4 cells by oscillating fluid flow. J. Bone Min. Res. 16 (S1): M192.

127. Reilly, G.C., Haut, T.R., Yellowley, C.E., Donahue, H.J. and Jacobs, C.R. (2001) Manipulation of the glycocalyx of an osteocytic and an osteoblastic cell line does not affect the intracellular calcium increase in response to fluid flow. J. Bone Min. Res. 16 (S1): M194.

128. Yellowley, C.E., Hancox, J.C. and Donahue, H.J. (2001) Cell swelling activation of membrane currents, Ca2+ transients and regulatory volume decrease in bovine articular chondrocytes (BAC). J. Bone Min. Res. 16 (S1): SA153.

129. You, J., Jacobs, C.R. and Donahue, H.J. (2001) Oscillatory fluid flow induces calcium mobilization in osteoblastic cells via extracellular ATP/UTP interactions with P2Y receptors. J. Bone Min. Res. 16 (S1): SU200.

130. Haut, T.R., Yellowley, C.E., Donahue, H.J., Haut-Donahue, T.L. and Jacobs, C.R. (2001) Mechanosensitivity of bone cells to oscillating fluid flow (OFF) depends on both shear stress

34

and flow rate. J. Bone Min. Res. 16 (S1): SU202.

131. Haut-Donahue, T., Donahue, H.J., Jacobs, C. and Yellowley, C. (2001) Annexin V contributes to mechanotransduction in osteoblast-like cells. 48th Annual Meeting of the Orthopaedic Research Society.

132. Haut, T., Donahue, H.J, Haut-Donahue, T., Yellowley C. and Jacobs, C.R. (2001) Mechanosensitivity of bone cells to oscillating fluid flow induced shear stress may be modulated by chemotransport. 48th Annual Meeting of the Orthopaedic Research Society.

133. Blankenhorn, B.D., Donahue, H.J. and Jacobs, C.R. (2001) The role of gap junctional intercellular communication in mechanotransduction. 48th Annual Meeting of the Orthopaedic Research Society.

134. Saunders, M.M., Foust, T.L., You, J., Zhou, Z., Donahue, H.J., Pellegrini, V.D. Jr. (2001) Mechanical stimulation via oscillatory fluid flow upregulations osteoprotegerin production in human osteoblastic MG63 cells in vitro. 48th Annual Meeting of the Orthopaedic Research Society.

135. You, J., Jacobs, C.R., Steinberg, T.H. and Donahue, H.J. (2001) P2Y purinergic receptors are necessary for oscillatory fluid flow induced calcium mobilization in osteoblastic cells. 48 th Annual Meeting of the Orthopaedic Research Societ

136. Li, Z., Zhou, Z., Welch, D. and Donahue, H.J. (2001) Restoration of connexin 43 alters breast cancer cell metastatic potential to bone by down regulation of osteopontin and connexin 32. 48 th

Annual Meeting of the Orthopaedic Research Society.

137. Donahue, S., Donahue H.J. and Jacobs, C. (2001) Refractory period of fluid flow induced calcium signaling in osteoblastic cells. 48th Annual Meeting of the Orthopaedic Research Society.

138. Donahue, H.J., Li, Z., Zhou, Z., Kapoor, P., Welch, D.R. and Saunders, M.M. (2002) Gap junctions and bone metastasis. Era of Hope 2002 Department of Defense Breast Cancer Research Program Meetings.

139. Li, Z., Zhou. Z., Welch, D.R., and Donahue, H.J. (2002) Connexin 43 transfection decreases osteopontin expression in breast cancer cells and increases their gap junctional communication with bone cells. Proceedings of the American Association for Cancer Research 43:1566.

140. Mastro, A.M., Gay, C.V., Welch, D.R., Donahue, H.J., Jewell, J.L. and Harms, J.F. (2002) A role for osteoblast apoptosis in breast cancer osteolytic metastasis. Proceedings of the American Association for Cancer Research 43:1570.

141. Harms, J.F., Mastro, A.M., Gay, C.V., Donahue, H.J. and Welch, D.R. (2002) Detailing the spectrum of skeletal metastasis of MDA-MB-435 breast cancer cells while enhancing detection sensitivity using green fluorescent protein. Proceedings of the American Association for Cancer Research 43:5236.

142. Lohmann, C.H., Schwartz, Z., Liu, Y., Albert, S.A., Simon, B.J., Sylvia, V.L., Dean, D.D.,

Bonewald, L.F., Donahue, H.J. and Boyan, B.D. (2002) Pulsed electromagnetic fields affect

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phenotype and connexin 43 protein expression in MLO-Y4 osteocyte-like cells and ROS 17/2.8 osteoblast-like cells. Amer. Soc. for Bone and Min. Res.

143. Lim, J.Y., Liu, X., Vogler, E.A.and Donahue, H.J. (2002) Adhesion and proliferation of human fetal osteoblastic (hFOB) cells on surfaces with varying surface energy. Tissue Engineering.

144. Brown, K.M., Saunders, M.M., Lynn, A.K., Goodspeed, D.C., Kirsch, T., Donahue, H.J. and Reid, J.S. (2003) The effect of COX-2 specific inhibitors on fracture healing in the adult rat femur. 49th Annual Meeting of the Orthopaedic Research Society.

145. Li, Z., Zhou, Z., Matters, G.L., Kapoor, P., Bond, J.D., Welch, D.R and Donahue, H.J. (2003) Osteoblastic cells decrease matrix metalloproteinase 1 expression by breast cancer cells: a mechanism for protection against bone metastasis? 49th Annual Meeting of the Orthopaedic Research Society.

146. Saunders, M.M., You, J., Li, Z., Pellegrini, V.P. and Donahue, H.J. (2003) Substrate deformation upregulates OPG/RANKL ratio in osteoblastic MG-63 cells. 49th Annual Meeting of the Orthopaedic Research Society.

147. Godin, L.M., Donahue, H.J., Jacobs, C.R.and Donahue, S.W. (2003) Calcium fingerprints in bone cell mechanotransduction. 49th Annual Meeting of the Orthopaedic Research Society.

148. Liu,X.,Vogler, E.A., Lim, J.Y. and Donahue, H.J. (2003) Influence of surface energetics on human fetal osteoblastic (hFOB) cell adhesion and proliferation. BioInterface 2003.

149. Donahue, H.J., Hansen, J.C., Lim, J.Y., Saunders, M.M., Yellowley, C.E., You, J., Siedlecki, C.A. and Vogler, E.A. (2003) Rational design of an optimized bioreactor for in vitro engineering of skeletal tissue. Innoventure , p47-48.

150. Lim, J.Y., Liu,X., Vogler,E.A. and Donahue, H.J. (2003) Effect of substratum surface characteristics on osteoblastic phenotypic characteristic. Engineering Tissue Growth.

151. Genetos, D., Geist, D., Donahue, H.J., and Duncan, R. (2003) Shear-induced nucleotide release regulates NF-B translocation and prostaglandin release in murine osteoblasts. Fifteenth Annual Graduate Student Research Forum.

152. Genetos, D., Geist, D., Donahue, H.J. and Duncan, R. (2003) Fluid shear induced nucleotide release mediates NFkappaB translocation in murine osteoblasts.

Eighteenth Annual Graduate Exhibition.

153. Hansen, J.C., Siedlecki, C.A. and Donahue, H.J. (2003) Surface morphological changes in bioglass? Assessed by In Situ Atomic Force Microscopy. Twenty-Ninth Annual Meeting of the Society for Biomaterials.

154. Hansen, J.C., Siedlecki, C.A. and Donahue, H.J. (2003) Surface morphological changes in bioglass? and compliance imaging of osteoblasts by atomic force microscopy. Fifteenth Annual Graduate Student Research Forum.

155. Hansen, J.C., Siedlecki, C.A. and Donahue, H.J. (2003) Measuring the stiffness of osteoblasts

36

by atomic force microscopy. Eighteenth Annual Graduate Exhibition.

156. You, J. and Donahue, H.J. (2003) Expression of P2Y purinergic receptors during osteoblastic differentiation. Amer. Soc. For Bone and Min. Res.

157. Kapoor, P., Li, Z., Zhou, Z., Donahue, H.J. and Welch, D. (2003) Metastatic breast cancer cells reduce osteoprotegerin secretion by human osteoblastic cells. Amer. Soc. For Bone and Min. Res.

158. Lim, J.Y., Vogler, E.A., Donahue, H.J. (2004) Integrin expression and osteopontin regulation in human fetal osteoblastic cells mediated by substratum surface characteristics, 50 th Annual Orthopaedic Research Society, p43, March 7-10, 2004, San Francisco, California, USA.

159. You, J. and Donahue, H.J. (2004) Effects of differentiation and parathyroidhHormone on expression of P2Y purinergic receptors in osteoblastic MC3T3-E1cells."

50th Annual Meeting of the Orthopaedic Research Society. Vol. 29: p135.

160. Li, Z, Zhou, Z. and Donahue, H.J. (2004) Increase in osteopontin in breast cancer cells are downstream of decreases in connexin 43 expression and contribute to breast cancer cell metastatic potential to bone ? 50th Annual Orthopaedic Research Society Annual Meeting, March 6 -11. 2004, San Francisco,U.S.A

161. Lim, J.Y., Donahue, H.J., (2004) Biomaterial characteristics important to skeletal tissue engineering, 34th International Sun Valley Workshop on Skeletal Tissue Biology, August 1-4, 2004, Sun Valley, Idaho, USA.

162. Riddle, R.C., Taylor, A.F., Genetos, D.C., Donahue, H.J., (2004) Mechanosensitity of human mesenchymal stem cells. 26th Annual Meeting of the American Society for Bone and Mineral Research. October 2004. Seattle, WA

163. Genetos, D.C. Donahue, H.J., Duncan, R.L. (2004) Fluid Flow-induced ATP Release Occurs by a Vesicular Mechanism and Mediates Prostaglandin Release. 26th Annual Meeting of the American Society for Bone and Mineral Research. October 2004. Seattle, WA

164. Strohbach, C.A., Genetos, D.C., Taylor, A.F., Donahue, H.J. (2004) Differentiation Affects MC3T3-E1 Mechanoresponsiveness and P2Y2 Expression. 26th Annual Meeting of the American Society for Bone and Mineral Research. October 2004. Seattle, WA.

165. Liu, X., Lim, J.L., Donahue, H.J., and Vogler, E.A. (2004) Influences of substratum surface characteristics on osteoblast adhesion, morphology, and integrin expression, The 7th New Jersey Symposium on Biomaterials Science, October 21-22, 2004, New Brunswick, New Jersey, USA.

166. Kim, Y.S., Lim, J.Y., Donahue, H.J. and Lowe, T.L. (2004) Non-invasive harvesting of osteoblastic cell sheet on a Thermo-Responsive Terpolymeric Film, Biomedical Engineering

Society 2004 Annual Fall Meeting, p61, October 13-16, 2004, Philadelphia, Pennsylvania, USA.

167. Shaughnessy, M.C., Lim, J.Y., Hansen, J.C., Siedlecki, C.A., Runt, J., Donahue, H.J. (2004) Human fetal osteoblastic cell response to polymer-demixed nanotopography, Biomedical

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Engineering Society 2004 Annual Fall Meeting, p51, October 13-16, 2004, Philadelphia, Pennsylvania, USA.

168. Hansen, J. C., Lim, J.Y., Siedlecki, C.A., Donahue, H.J. (2004) Determining the elastic modulus of osteoblasts on different surfaces by atomic force microscopy, Biomedical Engineering Society 2004 Annual Fall Meeting, p43, October 13-16, 2004.

169. Huang, X., Zhang, Y., Donahue, H.J. and Lowe, T.L. (2004).Novel porous hydrogel scaffold for three-dimensional culture of chondrocytes, Biomedical Engineering Society 2004 Annual Fall Meeting, October 13-16, 2004,

170. Lowe, T.L., Donahue, H.J., Kim, Y.S. and Lim, J.L. (2004) Multi-functional spin-cast films for promoting osteoblastic cell growth and facilitating non-invasive cell sheet harvesting”, AIChe Annual Meeting, November 7-12, 2004, Austin, Texas, USA.

171. Huang, X., Zhang, Y., Donahue, H.J. and Lowe, T.L. (2004). Three-dimensional culture of chondrocytes in porous hydrogel scaffold based on poly(N-isopropylacrylamide), poly(lactic acid) and dextran, AIChE Annual Meeting, Austin, TX, Nov 2004.

172. Huang, X., Zhang, Y., Donahue, H.J., Lowe, T.L. (2004). Novel porous thermoresponsive-co-biodegradable hydrogels as cartilage tissue engineering scaffolds, Polymer Network 2004 Conference, Bethesda, MD, Aug 2004.

173. Zou, J. and Donahue, H.J. (2005) Age-related Increases in Bone Cell Mechanosensitivity. 51st Annual Meeting of the Orthopaedic Research Society. Washington, DC. February 19-23, 2005, Washington D.C.

174. Li, Z., Zhou, Z, and Donahue, H.J. (2005) Modulation of connexin expression alters the osteoblastic phenotype and metastastic potential of breast cancer cells. 51st Annual Meeting of the Orthopaedic Research Society. Washington, DC. February 19-23, 2005, Washington D.C. U.S.A

175. Zhang, Y., Huang, X., Lowe, T., Donahue, H.J. (2005). Novel Biodegradable-Co-Thermosensitive Hydrogels For Cartilage Tissue Engineering Scaffolds. 51st Annual Meeting of the Orthopaedic Research Society. Washington, DC. February 19-23, 2005, Washington D.C.

176. Zou, J. and Donahue, H.J. (2005) Rat Osteoblastic Cells (ROB) Isolated from Different Aged Rats as a Model to Study Age-related Changes in Bone Mechanotransduction. March, 2005. 20th Annual Graduate Exhibition, Penn State University.

177. Lowe, T.L., Donahue, H.J., Huang, X. and Zhang, Y. (2005). Multifunctional Hydrogels as Bioreactors for Cartilage Repair. Innoventure 2005, Hershey, PA, April 2005.

178. Taylor, A. F., Genetos, D.C., Strohbach, C., Davis, C. M., Parrish, W. M., Donahue, H.J. (2005) Differentation dependent immediate and longterm effects of fluid flow on primary human osteoblasts. 51st Annual Meeting of the Orthopaedic Research Society.

179. Kephart, C.J., Genetos, D.C. and Donahue, H.J. (2005) Fluid flow activation of gap junction hemi channels and ATP release in MLO-Y osteocytes requires PKC activation ATP, fluid flow;

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gap junction; mechanotransduction; osteocyte; protein kinase 51st Annual Meeting of the Orthopaedic Research Society.

180. Lim, J.Y., Hansen, J.C., Siedlecki, C.A., and Donahue, H.J. (2005) Polymer demixed nanotopographic surfaces provide nonbiological cell-stimulating cues. The 230th National Meeting of the American Chemical Society.

181. Lim, J.Y., Hansen, J.C., Siedlecki, C.A., Hengstebeck B., Cheng J., Winograd N., and Donahue,

H.J. (2005) Poly(L-lactic acid) air-interface segregation in poly(L-lactic acid)/polystyrene thin film blends. The 230th National Meeting of the American Chemical Society.

182. Riddle, R.C., Taylor, A.F., Donahue, H.J. (2005) ATP Release mediates fluid flow-induced proliferation of human mesenchymal stem cells. 27th Annual Meeting of the American Society for Bone and Mineral Research, Nashville, TN.

183. Zhang, Y., Taylor, A.F., Genetos, D.C., Zhou, Z., Stanley, B.A. and Donahue, H.J. (2005) Proteomic study of osteoblast differentiation using isotope-coded affinity tags and Maldi-Tof analysis. 27th Annual Meeting of the American Society for Bone and Mineral Research, Nashville, TN.

184. Zhang, Y., Taylor, A.F., Stanley, B.A. and Donahue, H.J. (2006) Proteomic study of osteoblast differentiation using isotope-coded affinity tags and Maldi-Tof analysis. 52nd Annual Meeting of the Orthopaedic Research Society, Chicago IL.

185. Li, Z., Zhou, Z., Suva, L.J. and Donahue, H.J. (2006) Connexin 43 transfection alters breast cancer metastatic potential to bone by down regulation of FAK and Paxillin. 52nd Annual Meeting of the Orthopaedic Research Society, Chicago IL.

186. Shingle, D., Taylor, A.F., Paul, E.M., Saunders, M.M. and Donahue, H.J. (2006) Osteocytes are unique in their ability to communicate mechanical signals to osteoblasts. 52nd Annual Meeting of the Orthopaedic Research Society, Chicago IL.

187. Rogers, J.R., Riddle, R.C., Taylor, A.F. and Donahue, H.J. (2006) Release of ATP is responsible for fluid flow-induced proliferation of human mesenchymal stem cells. 52nd Annual Meeting of the Orthopaedic Research Society, Chicago IL.

188. Lim, J.Y., Dreiss, A.D., Riddle, R.C., Taylor, A.F., Hansen, J.C., Siedlecki, C.A. and Donahue,

H.J. (2006) Nanotextured surfaces are biomimetic in stimulating osteoblastic differentiation of stem cells. 52nd Annual Meeting of the Orthopaedic Research Society, Chicago IL.

189. Taylor, A. F., Riddle, R. C., Shingle, D. L., Donahue, H.J. (2006) Alterations In Gap Junctional Intracellular Communication During The Osteoblastic And Adipogenic Differentiation of Human Mesenchymal Stem Cell. 28th Annual Meeting of the American Society for Bone and Mineral Research, Philadelphia, PA.

190. Shingle DL; Taylor, AF; Paul, EM; Saunders, MM; Donahue, HJ. (2006) Osteocytes are unique in their ability to communicate mechanical signals to osteoblasts. 28th Annual Meeting of the American Society for Bone and Mineral Research, Philadelphia, PA.

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191. Lim, J.Y., Dreiss, A.D., Hansen, J.C., Siedlecki, C.A. and Donahue, H.J. (2007). Nanoscale topography affects integrin mediated osteoblast focal adhesion and Focal Adhesion Kinase Expression. 53rd Annual Meeting of the Orthopaedic Research Society (ORS), Paper#1574, San Diego, CA.

192. Li, Z., Zhou, Z. and Donahue, H.J. (2007). Restoring Connexin 43 expression reduces cadherin expression and metastatic potential of breast cancer cells. 53rd Annual Meeting of the Orthopaedic Research Society (ORS), Poster#971, San Diego, CA.

193. Riddle RC, Donahue HJ. (2007) “Fluid Flow-induced ATP Release Stimulates Human Mesenchymal Stem Cell Proliferation via Intracellular Calcium Release and Calcineurin Activation.” 53rd Annual Meeting of the Orthopaedic Research Society. February 2007, San Diego, CA.

194. Hippe KR, Riddle RC, Donahue HJ. (2007) “Chemotransport Contributes to the Effect of Fluid Flow on Human Mesenchymal Stem Cell Proliferation.” 53rd Annual Meeting of the Orthopaedic Research Society. February 2007, San Diego, CA.

195. Zhang Y, Taylor AF, Paul EM, Davison A, Bronson, S, Donahue, HJ. Inadequate bone adaptation in bone cell specific connexin 43 deficient mice. ASBMR 29th Annual Meeting, Hawaii, September 16-19, 2007.

196. Li Z., Li F., Zhou Z., Niyibizi C., Donahue H.J. (2008) Increased expression of connexins and cadherins in preferentially bone homing mesenchymal stem cells. 54th Annual Meeting of the Orthopaedic Research Society . San Francisco, CA

197. Lim J.Y., Salvi J.D., Riddle R.C., Donahue H.J. (2008) Nanoscale substrate topography regulates stem cell mechanosensitivity. 54th Annual Meeting of the Orthopaedic Research Society . San Francisco, CA

198. Updegrove G.F., Li Z., Zhou Z., Donahue H.J. (2008) The metastasis-suppressor gene, BRMS1, enhances breast cancer cell adhesion to bone and endothelial cells. 54th Annual Meeting of the Orthopaedic Research Society . San Francisco, CA

199. Zhang Y, Taylor A.F., Paul E.M., Davison A., Bronson S.K., Donahue H.J. (2008) Specific deletion of connexin 43 in osteoblasts and osteocytes results in osteopenia. 54th Annual Meeting of the Orthopaedic Research Society . San Francisco, CA

200. Li Z., Zhou Z., Welch D.R., Donahue H.J. (2008) Gap junction connexin 43 and breast cancer cell metastasis. 54th Annual Meeting of the Orthopaedic Research Society , San Francisco, CA

201. Gnerlich, M, Zhang, W, Donahue H.J., Voloshin A, Tatic-Lucic S. Novel MEMS-Based technology for measuring the mechanical properties of a live biological cell (2008), 1st International IEEE Conference on Microwaves, Communications, Antennas and Electronic

Systems (IEEE COMCAS 2008). Tel Aviv, Isarel.

202. Zhang Y, Paul E, Donahue HJ. (2009) Connexin 43 in Osteocytic Cells Regulates Osteoclastogenesis by Altering RANKL/OPG Expression Ratio. 55th Annual Meeting of the Orthopaedic Research Society, Les Vegas, NV.

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203. Salvi J, Lim JY, Zhang Y, Yanoso J, Niyibizi C, Donahue HJ. (2009) Culture On Specific NanoscaleTopographies Selects For Subpopulations Of Stem Cells With Increased Osteogenic Potential. 55th Annual Meeting of the Orthopaedic Research Society, Les Vegas, NV.

204. Pylawka TK, Donahue HJ. Effects of Nanotopography on Chondrogenesis of Human Mesenchymal Stem Cells. International Cartilage Research Society. May 2009.

205. Zhang Y, Paul E, Grigoryeva L, Donahue HJ. (2010) Connexin 43 in Osteocytes but not Osteoblasts Regulates Osteoclast Formation and Bone Resorption via the RANKL Pathway. 56 th Annual Meeting of the Orthopaedic Research Society, New Orleans, LA.

206. Xing Y,Gu Y, Paul E, Donahue HJ, You J. (2010) Mice Lacking P2Y2 Purinoceptors Exhibit Weaker Bones. 56th Annual Meeting of the Orthopaedic Research Society, New Orleans, LA.

207. Lim JY, Salvi JD, Donahue HJ. Controlling stem cell mechanosensitivity by nanotopographic culture. 2010 Society for Biomaterials (SFB), #397, April 21-24, 2010, Seattle, WA, USA.

208. Loiselle AE, Billingsley A, Paul EM, Zhang Y, Donahue HJ. (2010) Loss of Connexin43 in Mature Osteoblasts and Osteocytes Results in Delayed Mineralization and Bone Remodeling During Fracture Healing. Annual Meeting of the ASBMR, October 15-19, 2010, Toronto, Canada.

209. Chin-Quee K, Donahue HJ. (2010) Breast Cancer Cell Conditioned Media Decreases Release of CXCL5 by Osteoblastic Cells. Annual Meeting of the ASBMR, October 15-19, 2010, Toronto, Canada.

210. Pylawka TK, Hazard SW, Lewis GS, Reid SR, Donahue HJ, Johnson ST. Chondrocyte-based Repair of Articular Cartilage to Subchondral Bone. Northern Society of Plastic Surgeons. Oct. 2010

211. Pylawka TK, Lewis GS, Hazard SW, Donahue HJ, Reid SR, Johnson ST. Characterization of the Interface of the Bond Formed Between Native Cartilage, Engineered Cartilage and Subchondral Bone. Presented at the 38th Annual Midwest Connective Tissue Workshop, Chicago IL, May 2010.

212. Pylawka TK, Lewis GS, Hazard SW, Donahue HJ, Reid SR, Johnson ST. Characterization of the Bond Formed Between Native and Engineered Cartilage and Subchondral Bone. AAOS. Feb. 2011.

213. Loiselle AE, GS Lewis, A Billingsley, EM Paul, HJ Donahue. Nanotopographic Biofilms Enhance Osteoblast Differentiation and Mineralized Matrix Production During Bone Isograft Healing. 57 th Annual Meeting of the Orthopaedic Research Society. Long Beach, CA, 2011.

214. Loiselle AE, A Billingsley, EM Paul, Y Zhang, HJ Donahue. 2011. Decreased Bone Formation and Remodeling Following Femur Fracture in Cx43 deficient mice. 57th Annual Meeting of the Orthopaedic Research Society. Long Beach, CA, 2011.

215. Govey, PM, Jacobs, JM, Angel, TE, Karin, NJ, Donahue, HJ. Integrative proteomic analysis of mesenchymal stem cells reveals novel protein regulation by fluid flow. 57th Annual Meeting of the Orthopaedic Research Society. Long Beach, CA, 2011.

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216. Loiselle AE, Paul E, Lewis G, Donahue HJ. Decreased Bone Formation and Bone Resorption Following Fracture in Osteoblast-Osteocyte Specific Connexin 43 Deficient Mice. Annual Meeting of the ASBMR, San Diego, CA, 2011.

217. Govey P, Karin NJ, Donahue HJ. Osteogenic Transcriptome Regulation in MLO-Y4 Osteocytes Exposed to Fluid Flow. Annual Meeting of the ASBMR, San Diego, CA, 2011.

218. Lloyd SA, Paul E, Zhang Y, Donahue HJ. Increased Sensitivity to Skeletal Unloading in Connexin43-deficient Mice. Annual Meeting of the ASBMR, San Diego, CA, 2011.

219. Lloyd SA, Zhang Y, Paul EM, Burton S, Donahue HJ. Mice with Conditional Knockout of Connexin43 Have Greater Femoral Strength Than Wild Type Controls Following Three Weeks of Mechanical Unloading via Hindlimb Suspension. Annual Meeting of the ORS, San Francisco, CA, 2012.

220. Zhang Y, Paul EM, Sternlieb M, Donahue, HJ. Osteoclast Specific Connexin 43 Deletion Results in Decreased in vivo Osteoclastogenesis. Annual Meeting of the ORS, San Francisco, CA, 2012.

221. Govey PM, Jacobs JM, Karin NJ, Donahue HJ. Transcriptome Analysis Reveals Novel Inflammatory Response of Osteocytes to Fluid Flow. Annual Meeting of the ORS, San Francisco, CA, 2012.

222. Loiselle AE, Wei L, Lewis GS, Paul EM, Lakhtakia A, Donahue HJ. Specific Biomimetic Hydroxyapatite Nanotopographies Enhance Bone Isograft Osteointegration. Annual Meeting of the ORS, San Francisco, CA, 2012.

223. Sathyendra V, Donahue HJ, Vrana KE, Berg A, Reid JS. Single Nucleotide Polymorphisms in Osteogenic Genes in Atrophic Delayed Fracture Healing. Annual Meeting of the ORS, San Francisco, CA, 2012.

224. Lloyd SA, Lewis GS, Zhang Y, Paul EM, Donahue HJ. Connexin 43 Deficiency Protects Against Skeletal Changes Associated with Mechanical Unloading. Annual Meeting of the ASBMR, Minneapolis, MN, 2012.

225. Loiselle AE, Paul EM, Lewis GS, Donahue HJ. Loss of Osteoblastic Connexin 43 Results in Delayed Bone Formation, Increased Sclerostin Expression and Attenuated Wnt Signaling During Fracture Repair. Annual Meeting of the ASBMR, Minneapolis, MN, 2012.

226. Loiselle AE, Paul EM, Lewis GS, Donahue HJ. Enhanced Sclerostin Expression Delays Mineralization and Decreases Strength of the Fracture Callus in Osteoblast/Osteocyte Specific Cx43

Deficient Mice. Annual Meeting of the Orthopaedic Research Society. San Antonio, TX, 2013.

227. Lloyd SA, Zhang YZ, Lewis GS, Paul EM, Donahue HJ. Connexin 43 Deficiency Attenuates Bone Loss and Increased Sclerostin Production during Unloading. Annual Meeting of the Orthopaedic Research Society. San Antonio, TX, 2013.

228. Vakharia K, Pylawka T, Lewis G, Donahue H, Reid JS, Johnson TS. Integrative Repair of Knee Articular Defects with Tissue Engineered Cartilage. Robert H. Ivy Society Meeting of Plastic Surgeons 59th Annual Meeting, 2013.

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229. Loiselle A, Lloyd SA, Paul EM, Lewis GS, Donahue HJ. Inhibition of GSK3-β Rescues the Impairments in Bone Formation and Mechanical Properties Associated with Fracture Healing in Osteoblast/ Osteocyte-Selective Connexin 43 Deficient Mice. Annual Meeting of the ASBMR, Baltimore, MD, 2013.

230. Lloyd SA, Zhang Y, Paul EM, LaufenbergL, Lang CH, Donahue HJ. Interdependence of Muscle and Bone Loss Induced by Disuse and Aging. Annual Meeting of the ASBMR, Baltimore, MD, 2013.

231. Lloyd SA, Loiselle AE, Zhang Y, Donahue HJ. Evidence for a Protective Effect of Connexin 43

Deficiency in the Development of Osteocytic Osteolysis During Skeletal Unloading. NASA Human Research Program Investigators' Workshop. Galveston TX 2014.

232. Govey PM, Zhang Y, Paul EM, Donahue HJ. Quantification of Tibial Adaptation to Cantilever

Loading via MicroCT. Annual Meeting of the Orthopaedic Research Society. New Orleans, LA 2014.

233. Xing Y, Donahue HJ, You J. Oscillatory Fluid Flow Induced Autophagy in Bone Cells. Annual Meeting of the Orthopaedic Research Society. New Orleans, LA 2014.

234. Loiselle AE, Xing Y, You J, Donahue HJ, Zhang Y. Connexin 43 Regulates Chondrocyte Differentiation. Annual Meeting of the Orthopaedic Research Society. New Orleans, LA 2014.

235. Krause, A, Speacht, T., Govey, P., Zhang, Y., Steiner, J., Lang, C., and Donahue, H.J. Sarcopenia and Increased Body Fat in Sclerostin Deficient Mice. Annual Meeting of the ASBMR, Houston TX, 2014.

236. Zhang, Y., Loiselle, A.,Yanghue, X. You, J., and Donahue, H.J. Connexin 43 Regulates Chondrocyte Differentiation. Annual Meeting of the ASBMR, Houston TX, 2014.

237. Cho, E., Zhang, Y., Lang, C., Pruznak, A., Donahue H.J. and Kim, H.M. Effect of Tamoxifen on Fatty

Degeneration of Rotator Cuff Muscles In Chronic Rotator Cuff Tear: An Animal Model Study. Annual Meeting of the Orthopaedic Research Society, Las Vegas, NV 2015.

238. Govey, P.M. and Donahue, H.J. Mechanical Loading Attenuates Radiation-Induced Bone Loss in

Bone Marrow Transplanted Mice. Annual Meeting of the Orthopaedic Research Society, Las Vegas, NV 2015.

239. Govey, P.M. and Donahue, H.J. Mechanical Loading Attenuates Radiation-Induced Bone Loss in Mice. NASA Human Research Program (HRP) Investigator’s Workshop, January 13-15, 2015, Galveston, TX.

240. Govey, P.M., Imamura, Y. and Donahue, H.J. RNA-Seq Reveals Novel Mechanosensitive Genes in Fluid Flowed Osteocytic MLO-Y4 Cells. BMES Cell and Molecular Bioengineering (CMBE)

Conference, January 6-10, 2015, St. Thomas, USVI.

241. Speacht, T., Krause, A.R., Steiner, J.L., Lang, C.H. and Donahue, H.J. Hindlimb Suspension Plus Immobilization Exaggerates Sarcopenia but not Osteopenia. Annual Meeting of the American Society for Bone and Mineral Research, October 9-12, 2015, Seattle, WA.

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242. Speacht, T., Krause, A., Steiner, J.L., Lang, C.H., Donahue, H.J. Sarcopenia but not Osteopenia is Exaggerated by Simulated Microgravity and Immobilization. American Society for Gravitational and Space Research (ASGSR) 31st annual meeting in November 11-14th, Alexandria, VA.

243. Govey, P.M., Zhang, Y., Donahue, H.J. Mechanical Loading is an Effective Countermeasure for

Radiation-Induced Bone Loss in Mice. American Society for Gravitational and Space Research (ASGSR) 31st annual meeting in November 11-14th, Alexandria, VA.

244. Govey, P.M., Kawasawa, Y.I., Donahue, H.J. Mapping the Osteocytic Cell Response to Fluid

Flow Using RNA-Seq. Annual Meeting of the Orthopaedic Research Society. Orlando, FL 2016.

245. Jiang, P., Zhou, Z., Zhang, X., Donahue, H.J., Wang, Y. Porous extracellular matrix mimic for functionalization of bone allografts. Annual Meeting of the Orthopaedic Research Society. Orlando, FL 2016.

246. Speacht, T.L., Krause, A.R., Steiner, J.L., Lang, C.H., Donahue, H.J. Muscle and Bone Loss in Response to Unloading are Uncoupled. Annual Meeting of the Orthopaedic Research Society. Orlando, FL 2016.

247. Mellor, L.F., Trevisan, B., Taylor, M.A., Krause, A.R., Donahue, H.J., Oxford, J.T., Loboa, E.G. Sclerostin inhibition in hindllimb unloading model: good news for bone, bad news for cartilage. 2016 NASA Human Research Program Investigators’ Workshop. February 8-11, 2016, Galveston, TX

248. Donahue, HJ, Speacht, TL, Krause, AR, Lloyd, SA, Zhang, Y, Laufenberg, LJ, Steiner, JL, Lang, CH. Integrated Regulation of Bone and Muscle Metabolism by Simulated Microgravity. 2016 NASA Human Research Program Investigators’ Workshop. February 8-11, 2016, Galveston, TX

249. Govey, PM, Zhang, Y, Donahue HJ, Radiation-induced bone loss is attenuated by mechanical loading. Annual Meeting of the American Society for Bone and Mineral Research. September 19, Atlanta, GA.

250.

Invited Lectures: 1. Endocrine Research Unit, Mayo Clinic, 1987: (Ca2+Mg2+)-ATPase Kinetics: Regulation by calcium

and calmodulin. 2. Endocrine Research Unit, Mayo Clinic, 1988: Effect of parathyroid hormone on second messenger

activity in bone cells. 3. Endocrine Research Unit, Mayo Clinic, 1988: PTHrP: A novel regulator of bone cell activity. 4. Endocrine Research Unit, Mayo Clinic, 1989: Effects of PTHrP on cytosolic ionized calcium and

cAMP in ROS 17/2.8 cells: Structure-function studies using aequorin luminescence.

44

5. Department of Orthopaedic Surgery, SUNY, Stony Brook, 1989: Hormonal regulation of calcium and bone metabolism.

6. Department of Surgery, SUNY, Stony Brook, 1990: Hormonal and local regulation of bone cell

activity. 7. New York Bone and Tooth Society, Rockefeller University, N.Y., 1991: Bone cell signal transduction. 8. Department of Rheumatology, Veterans Administration Hospital, Northport, NY, 1991: Bone

formation and resorption. 9. American Federation for Aging Research Grantee Conference, 1991, Briarcliff Manor, N.Y. 10. Brookhaven Memorial Hospital, Patchogue, N.Y., 1992: Cell biology of the bone: implant interface.

11. Royal Danish Bone and Tooth Society, Aarhus, Denmark, 1992: Signal transduction mechanisms in

bone cells. 12. Department of Occupational and Physical Therapy, SUNY, Stony Brook, 1992: Basics of bone cell

biology. 13. Department of Orthopaedic Surgery, SUNY, Stony Brook, 1992: Age-related changes in bone cell

signal transduction. 14. Glaxo Research Institute, Research Triangle Park, NC, 1992: Intercellular and intracellular

communication in bone cell networks. 15. Gordon Research Conference on Bones and Teeth, Meriden, NH, 1993: Cell to cell communication

via gap junctions in osteoblastic networks. 16. Pennsylvania State University College of Medicine, Hershey, PA, 1993: Contributions of Bone Cell

Biology to Orthopaedics. 17. Endocrine Research Conference, SUNY, Stony Brook, 1993: Bone Cell Signal Transduction and

Related Topics. 18. Experimental Biology '94. Anaheim, CA, 1994: Spontaneous Ca2+ transients recorded from

osteoblastic cells: A real-time measurement of cellular responses to ELF fields. 19. Osteotech Inc. Shrewsbury, NJ, 1994: Osteoinductive Assays in Cell Culture Models. 20. International Conference on Calcium Regulating Hormones, Melbourne, Australia, 1995: Hormonal

regulation of cAMP in gap junction deficient osteoblastic cells. 21. University of Melbourne, The Royal Melbourne Hospital, Melbourne, Victoria, Australia, 1995: The

role of intercellular communication in bone cell networks. 22. Medical College of Georgia, Augusta, GA, 1995: The role of gap junctions in bone metabolism.

45

23. Pennsylvania State University College of Medicine, Hershey PA. 1995: Biophysical Signal Transduction in Bone.

24. Sun Valley Bone Biology Workshop, Sun Valley ID, 1995: Cell-to-cell Communication in Bone.

25. Indiana University School of Medicine, Indianapolis, IN, 1995: Characterization of Gap Junctions in

Bone. 26. Eli Lilly, Inc., Indianapolis, IN, 1995: PTH regulation of cell to cell communication. 27. Pennsylvania State University College of Medicine, Department of Biochemistry, Hershey, PA, 1995:

Intercellular Communication. 28. Pennsylvania State University College of Medicine, Department of Cell and Molecular Physiology,

Hershey, PA, 1995: The role of gap junctions in cellular networks.

29. The American Society of Cell Biology Meeting, Washington D.C., 1995, Invited speaker: Gap junctions and Biophysical signal Transduction in Bone.

30. Interface of Biomechanics and Cell Biology in Orthopaedics, Baltimore, MD, 1996, Invited Speaker: Gap Junctions and Mechanical Integration.

31. Impact of Modern Technology in Dentistry, Baltimore, MD, 1996. Invited Speaker: Gap Junction Expression and Tooth Movement.

32. Gordon Conference on Orthopaedics and Biomechanics, 1996: Mechano-Chemical Signal

Transduction in Bone. 33. Physiological Transport Studies Laboratory, Department of Chemical Engineering Seminar Series,

State College, PA,1996, Cell-to-cell communication and hormonal responsiveness in bone cells.

34. Gordon Conference on Bones and Teeth, 1997, Invited Speaker: Cell-to-cell Communication and

the Mechanostat Theory of Bone Adaptation. 35. University of Western Ontario, London, ON, Departments of Physiology and Biochemistry, 1997,

Gap Junctions and Signal Transduction in Bone. 36. International Union of Physiological Sciences, St. Petersburg, Russia, 1997: Gap junctional

intercellular communication in bone cells. 37. Pennsylvania State University, Hershey, PA. Cell and Molecular Biology Program 1997, Biophysical

Signal Transduction in Bone and Cartilage. 38. Biomedical Engineering Society Annual Fall Meeting, San Diego, CA, 1997, Gap Junctions and

Fluid Flow-Induced Shear Stress. 39. Advanced Tissue Sciences, San Diego, CA, 1998, Cellular networking in the musculoskeletal

system: Implications for tissue engineering.

46

40. International Workshop on Hard Tissue Biology, Sun Valley, ID, 1998, Gap junctions in osteocytes. 41. Third International Congress on Osteoporosis, Xi’an, P.R. China, March31-April 3, 1999,Osteocytic

gap junctional communication and biophysical communication in bone. 42. Grace Kimbal Memorial Lecture, Wilkes University. Wilkes-Barre, PA , April 14, 1999. Intercellular

communication and mechanotransduction in the Musculoskeletal system. 43. Pennsylvania State University College of Medicine, Hershey PA, January 12,2000:

Cell and Molecular Biology Program, Recent Developments in Gap Junctional Communication.

44. Pennsylvania State University College of Medicine, Hershey PA, February 25, 2000: Annual Meeting of the Intercollege Graduate Faculty in Physiology, The Role of Gap Junctions in Cellular Differentiation and Cancer.

45. Pennsylvania State University College of Medicine, Hershey PA, February 28, 2000: Department of

Pharmacology Seminar Series, Gap Junctional Communication. 46. Slippery Rock University, Slippery Rock, PA, March 31, 2000: Biological Sciences Seminar,

Musculoskeletal Cell Biology.

47. Institute of Environmental Medicine, University of Pennsylvania, Philadelphia, PA January 19, 2001,

Gap junctional Communication in Cell Differentiation and Cancer. 48. Department of Cell Biology and Neuroscience, Rutgers University, Piscataway, NJ, March 9, 2001,

Recent developments in gap junction function. 49. AOA Resident Research Conference, Hershey, PA. March 30, 2001. Research collaborations

between clinical and basic scientists. 50. Osteology 2001, Joint Meeting of Austrian and German Bone Societies Wiesbaden, Germany, April

5, 2001. Cell-Cell Communication in osteocytes.

51. National Institute on Aging Functional Senescence Workshop, University of Louisville, Louisville, Kentucky April 24, 2001. Age-Related Changes in Bone Cell Signal Transduction.

52. Pennsylvania State University Molecular Medicine Program Faculty Retreat, April 28, 2001.

Intercellular Communication and Breast Cancer Metastasis to Bone. 53. Department of Orthopaedics, Johns Hopkins University, Baltimore, MD, June 28, 2001. Intercellular

communication in bone physiology and pathophysiology.

54. Departments of Physiology and Geriatrics, University of Arkansas for the Medical Sciences, Little

Rock, AR, December 6, 2001. Gap junctions in bone health and disease. 55. 32nd International Sun Valley Hard Tissue Workshop, Sun Valley, Idaho, August 6, 2002.

Intercellular communication in breast cancer metastasis to bone. 56. National Institute on Aging Biology of Aging Program, Extracellular Matrix and Aging.

47

Musculoskeletal System and Skin Meeting, The National Conference Center at Lansdowne, VA, September 16, 2002. Intercellular junctions and age related changes in bone cell differentiation and mechanotransduction.

57. Department of Biochemistry and Molecular Biology, Pennsylvania State University College of

Medicine, Hershey, PA, January 13, 2003. Intercellular communication, cell differentiation and cancer.

58. Department of Surgery, Pennsylvania State University College of Medicine, Hershey, PA, January

15, 2003. Bone cell biology and functional tissue engineering. 59. The Welcome Trust, Broadway, Worcestshire, U.K. June 28-July 1, 2003. Why do people’s bones

break? The structural, mechanical and cellular basis of the gain and loss of bone strength during growth and ageing.

60. Department of Cellular and Integrative Medicine, Indiana University School of Medicine,

Indianapolis, IN. September 2, 2003. Intercellular communication in bone physiology and pathophysiology.

61. Nemours Biomedical Research, Alfred I. DuPont Hospital for Children, Wilmington, DL. February 23,

2004. Bone cell biology and functional tissue engineering. 62. University of California/Shriner’s Hospital, Sacramento, CA. March 10, 2004. Functional Tissue

Engineering of Bone. 63. 35th International Sun Valley Workshop On Skeletal Tissue Biology, Sun Valley, ID. August2, 2004.

Gap Junctions and Cancer Metastasis. 64. Sixth Bone Fluid Flow Workshop, Seattle, WA. September 30, 2004. Responsiveness of cellular

networks to fluid flow. 65. American Society for Bone and Mineral Research 26th Annual Meeting, Bone Remodeling and

Stress Fracture Working Group, Seattle, WA. October 2, 2004. Cell-to-cell communication networks.

66. Crossover 2004, University Park, Pennsylvania. October 20-21, 2005. Bionanotechnology and

skeletal tissue engineering. 67. Cancer Interaction with the Bone Microenvironment, NIH Tumor Microenvironment Workshop,

Washington, D.C. February 27, 2005. Gap junctions in mechanotransduction and metastasis. 68. Seventh Bone Fluid Flow Workshop, New York, NY. September 21, 2005. Fluid flow activation of

gap junctional communication and hemichannel activity in bone cells. 69. Biomedical Engineering Society Annual Fall Meeting, Baltimore, MD. September 29, 2005. Fluid

flow activation of osteocytic to osteoblastic communication. 70. University of Alabama, Birmingham, Departments of Pathology, Genomics and Pathobiology,

February 23, 2006. Intercellular Communication in Bone.

48

71. Novartis Institute of Biomedical Research, Basel, Switzerland, February 28, 2006. Gap Junctional

Communication in bone. 72. The Graduate Program in Cell and Molecular Biology, Pennsylvania State University, January 18,

2006. Regenerative medicine, stem cells, nanotechnology and string theory: the future of cell and molecular biology.

73. University of California/Veterans Administration, San Diego, CA, April 17, 2006. Regenerative

Medicine in Musculoskeletal Tissue Engineering. 74. Medical College of Georgia, Augusta, GA, September 27, 2006. Regenerative Medicine and

Skeletal Tissue Engineering. 75. Lehigh University, Department of Mechanical Engineering, Bethlehem, PA, March 23, 2007.

Biophysical and Nanotopographic Regulation of Human Mesenchymal Stem Cell Differentiation. 76. Mechanotransduction in Mineralized Tissues, American Association of Dental Research Annual

Meeting, Dallas, Texas, Saturday, April 5, 2008, ATP and Bone Cell Mechanotransduction. 77. Ninth Bone Fluid Flow Workshop: Bone fluid flow, adaptation, and regeneration, Amsterdam, The

Netherlands, May 23, 2008, Optimizing stem cell potential for human bone regeneration.

78. Yale University, Core Center for Musculoskeletal Disorders, New Haven, Connecticut, January 29, 2009, Gap junctions and connexins in breast cancer metastasis to bone.

79. University of Pennsylvania, Core Center for Musculoskeletal Disorders, Philedelphia, Pennsylvania, February 16, 2010, Exploiting the physical environment to optimize stem cells for skeletal regeneration.

80. Spotlight Speaker: Optimizing Adult Stem Cells for Musculoskeletal Regeneration, (2010) 56th Annual Meeting of the Orthopaedics Research Society. New Orleans, LA.

81. Noll Laboratories, Penn State, University Park, Pennsylvania, March 19, 2010, Exercise and Bone: cellular response to mechanical load.

82. University of Delaware, Department of Biological Sciences, Newark, DE., April 7, 2010, Biophysical regulation of stem cell differentiation.

83. Organ and Tissue Awareness Projects’ Institute for Educators, State College, PA, May 12, 2010, Regenerative Medicine and the Future of Organ and Tissue Transplantation.

84. Thomas Jefferson University, Program in Tissue Engineering and Regenerative Medicine, Philadelphia, PA, June 21, 2010, Optimizing stem cells for skeletal regeneration.

85. Department of Orthopaedics, University of Pennsylvania Philadelphia, PA, November 16, 2010, Exploiting the physical environment to optimize stem cells for skeletal regeneration.

86. Penn State Cancer Institute, Hershey, PA. October 18, 2011, Bone Cancer and

49

Skeletal Regeneration.

87. Department of Cellular and Molecular Physiology, Penn State College of Medicine, Hershey, PA. November 15, 2011. Cellular mechanisms of bone adaptation to load.

88. University of Delaware, College of Health Sciences, Newark, DE. February 8, 2011, Physical cues

and skeletal regeneration.

89. Pennsylvania Policy Makers Education Seminar, Hershey PA., September 1, 2011, Preventing bone loss when we get older: lessons from space and my mom.

90. 2013 NASA Human Research Program Investigators’ Workshop, Galveston, TX, February 12, 2013. Cell-to-cell signaling and mechanotransduction in bone.

91. 2013 NASA Human Research Program Investigators’ Workshop, Galveston, TX, February 12, 2013. Integrated regulation of bone and muscle metabolism by simulated microgravity.

92. Department of Pharmacology and Physiology, UMDNJ - New Jersey Medical School. Newark, NJ, April 8, 2013. Gap junction function in bone.

93. Center for Healthy Aging, University of California, Davis, CA. April 23, 2013. Cx43 and bone adaptation to load.

94. Page Morton Hunter Distinguished Seminar, Clemson University Department of Bioengineering, Clemson, SC. December 5, 2013. Biophysical regulation of bone remodeling and regeneration.

95. Department of Biological Sciences, Lehigh University, Bethlehem, PA. January 22, 2014. Gap junctions and biophysical regulation of bone remodeling and regeneration.

96. Materials Research Institute, Penn State, University Park, PA. March 4, 2014. Using Nanotopography to Control Stem Cell Behavior and Skeletal Regeneration.

97. Department of Regenerative Medicine and Cell Biology, Medical University of South Carolina, Charleston, SC. August 20, 2014 Musculoskeletal Degeneration and Regeneration.

98. Department of Biomedical Engineering, Penn State, University Park, PA. September 3, 2014. Biophysical signals and musculoskeletal degeneration and regeneration.

99. Department of Biomedical Engineering Virginia Commonwealth University, Richmond, VA. September 17, 2014. Mechanobiology and Musculoskeletal Regenerative Medicine.

100. Department of Cellular & Molecular Physiology, Penn State Hershey, February 10, 2015, Convergent Approaches to Skeletal Regeneration.

101. Orthopaedic Research Society 2015 Annual Meeting Professional Advancement Session: Career Advancement: Winning the Uphill Battle for Research Funding. Las Vegas, NV. March 28, 2015. What to do if you don’t get funded or even discussed.

50

102. United States Bone and Joint Initiative, Young Investigators Initiative Workshop; Rosemont, IL. April 25, 2015. Keynote Speaker: Why a career in scientific research?

103. PSU Pathway to Partnerships (P³) symposium: A Collaborative Approach to Tissue Engineering Penn State, University Park, September 3, 2015. Exploiting Nanotechnology in Musculoskeletal Regeneration.

104. Louis V. Avioli Musculoskeletal Research Seminar, Washington University, St Louis, MO. April 7,

2016. Bone Adaption to its Biophysical Environment

105. William F. Neuman Seminar, Center for Musculoskeletal Research, University of Rochester,

Rochester, NY. May 4, 2016. Biophysical Regulation of Bone Metabolism.

106. Keynote Speaker, Virginia Academy of Sciences, 94th Annual Meeting, University of Mary Washington, Fredericksburg, VA. May 19, 2016. Biophysical Regulation of Bone Metabolism.

107. Invited Speaker, National Academy of Engineering, Committee Roundtable on Biomedical Engineering Materials and Applications (BEMA) "Orthopedic Biomaterials and Products” Jonsson Center, National Academies Woods Hole, MA (Carriage House) June 14-15, 2016. Optimizing biomaterials for stem cell differentiation and bone regeneration.

108. Visiting Professor, Department of Orthopaedic Surgery, University of Virginia, Charlottesville, VA, July 26-27, 2016. Biophysical Regulation of Bone.

109. Visiting Professor, Center for Musculoskeletal Disorders, University of Pennsylvania, Philadelphia, PA, October 25, 2016. Biophysical Regulation of Bone.

110. Guest Lecturer, Department of Physiology and Biophysics, Virginia Commonwealth University, Richmond VA. October 27, 2016. Musculoskeletal Adaptation to Space Flight.

111. Guest Lecturer, Division of Endocrinology and Metabolism, Virginia Commonwealth University, Richmond VA. November 16, 2016. Musculoskeletal Adaptation to Space Flight.

112. Guest Lecturer, M.D./Ph.D. Program. Virginia Commonwealth University, Richmond VA. November 16, 2016. Musculoskeletal Adaptation to Space Flight.

113. Visiting Professor, Department of Biomedical Engineering, University of Virginia, Charlottesville, VA, November 18, 2016. Musculoskeletal Adaptation to the Physical Environment.

Documents

CURRICULUM VITAE Name: Henry Joseph Donahue · CURRICULUM VITAE Name: Henry Joseph Donahue Address: Department of Biomedical Engineering School of Engineering Virginia Commonwealth