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Curriculum Vitae
Personal information
Name Diaeldin Ahmed Salih
Date and place of birth 31 July 1972, Atbara, Sudan
Nationality Sudanese
Religion Muslim
Marital status Married
Present affiliation
Associate Professor
Ministry of Livestock
Animal Resources Research Corporation
Nile college (Part time)
Department of Parasitology
Medical Laboratory Science Programme (MLSP)
Khartoum– Sudan
E-mail: [email protected]
Cell phone: +249-912649292
+249-123852358
Language skills
Arabic: first language
English: fluent, both in speaking and writing
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HigherEducation 1992 - 1997 Faculty of Veterinary Science – University of Nyala, Sudan. B.V.Sc. 1997 - 1999 Faculty of Science and Technology, Computer Studies Center,
University of Gezria, Diploma in Computer Science, Wad Madani, Sudan
2002 - 2004 Department of Parasitology – Faculty of Veterinary Medicine – University of Khartoum. M.V.Sc Thesis title (Epidemiological Studies on Tropical Theileriosis (Theileria annulata infection of cattle) in the Sudan).
2005 - 2008 Research Center Borstel, Department of Immunology and Cell Biology, Borstel, Germany and Sudan Academy of Sciences – PhD Thesis title (Molecular epidemiological studies on East Coast fever (Theileriaparva infection of cattle) in Central EquatoriaState, Southern Sudan.
Research appointments
Post Duration Institute Assistant Research Officer 01 Aug 1998- 01 March 2003 Central Veterinary Research Laboratories Research Officer 02 March 2003 – 23 Dec
2008 Central Veterinary Research Laboratories
Assistant Research Professor 24 Dec 2008- 31 Dec 2012 Veterinary Research Institute Associate Research Professor 1 Jan 2013 up to date Central Veterinary Research Laboratory
Administrative appointments Post Duration Place Director 10 February 2010 – 5
March 2013 Kassala Veterinary Research Laboratory
Deputy Director 25 March 2015 -1 September 2015
Animal Resources Research Corporation
Director of Directorate, Planning, extension
25 July 2017 to date Animal Resources Research Corporation
Post held
Post Duration Institute Place
Research Fellowship 14 Jan – 15 April 2004 University of Pretoria South Africa
PhD student 15 June 2005 – 31 Dec 2008 Borstel Research Center Germany
Visiting Scientist 15Aug - 15 Nov 2011 ILRI Kenya
Invited Scientist 20 Nov – 19 Dec 2011 Institute of Tropical Medicine(ITM) Belgium
Research Fellow 1 July - 30 Nov 2012 ILRI Kenya
Post doc 21 Nov – 20 Dec 2013 GADVAS University India
Visiting Scientist 1 April - 30 June 2014 ILRI Kenya
External Scientist 10 May –30 June 2016 Lanzhou Veterinary Research Institute China
Research visit 03-31 October 2017 Freie Universität Berlin Germany
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Courses
19 June – 15 July 1999 Course on Biostatistics and SPSS, Tropical Medicine Research
Institute, National Center for Research, Khartoum, Sudan.
17 May – 5 July 1999 Course on Basic Immunology, Central Veterinary Research
Laboratories, Khartoum – Sudan.
11 Sep – 6 Oct 2000 Group training course on Tsetse Management, International
Centre of Insect Physiology and Ecology (ICIPE), Nairobi, Kenya.
9-14 Aug 2003 Practical Training course on ELISA for Diagnosis of tick-borne
diseases, Central Veterinary Research Laboratories, Khartoum –
Sudan.
20-24 Nov 2006 Technical workshop on Molecular diagnostic tools for tick-borne
diseases. Organized by the International Consortium on ticks and
tick-borne diseases (ICTTD-3) Asian Component held in Pendik,
Istanbul, Turkey.
8-16 June 2008 ICTTD Bioinformatics workshop, a six-day practical course on
bioinformatics of ticks and tick-borne pathogens, University of
South Bohemia – Faculty of Science, and Biology Centre of the
Academy of Sciences – Institute of Parasitology, Czech Republic.
7 – 25 May 2012
19 – 23 Nov 2012
Genetic Resources and Intellectual Property Rights (GRIP)
Funded by The Swedish International Development Cooperation
Agency (Sida), held at Swedish University of Agricultural Sciences,
Uppsala, Sweden.
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Research grants April 2005 International Foundation for Science (IFS)
Research grant (36 months): “Epidemiological studies on East Coast fever (Theileria parva infection of cattle) in Southern Sudan.”
12,000 US$
http://ifs.se/ June 2006 Integrated Control of TicksandTick-borneDiseases (ICTTD-3).
Research visit to Research Center Borstel Department of Immunology and Cell Biology, Borstel, Parkallee 22, 23845 Borstel, Germany (6 months).
10,000 EURO
http://www.tickbornezoonoses.org/joomla/
October 2009 International Foundation forScience (IFS) Researchgrant (36 months): “Characterization of field isolates of Theileria parvafrom Southern Sudan.” 12,000 US$
http://ifs.se/ June 2010 “Molecularepidemiologynetworkfor promotion and support of
delivery of life vaccines againstTheileriaparvaandTheileriaannulatainfection in EasternandNorthernAfrica (AH 41/7-1) ”. First phase (3 years)
(Subcontractor: German Research Foundation ‘DFG’ Germany) http://theileriainafrica.weebly.com/ 33,000 EURO
August 2014 “Improvment of currentand development of new vaccines fortheileriosisandbabesiosis of small ruminant “PiroVac” (Subcontractor: EU-funded research programme) (6 months)
http://www.theileria.org/pirovac/index.htm 25,800 EURO
December 2014 “Molecularepidemiologynetworkfor promotion and support of delivery of live vaccines againstTheileriaparvaandTheileriaan-nulatainfection in EasternandNorthernAfrica (SE862/2-1)” Second phase (3 years)
(Subcontractor: German Research Foundation ‘DFG’ Germany) http://theileriainafrica.weebly.com/ 50,400 EURO
December 2017 “Molecular epidemiology network for promotion and support of delivery of live vaccines against Theileria parva and Theileria annulata infection in Eastern and Northern Africa (CL 166/4-2) ”Third phase (2 years)(Subcontractor: German Research Foundation ‘DFG’ Germany) http://gepris.dfg.de/gepris/person/357668636
62,800EURO
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Fellowships 15 Jan – 14 April 2004 International Atomic Energy Agency (IAEA)
Fellowship on the use of molecular biology
techniques in diagnosis of tick-borne diseases.
University of Pretoria–Faculty of Veterinary
Science–Department of Veterinary Tropical
Diseases–Pretoria–South Africa.
1 Oct – 31 Dec 2007 International Atomic Energy Agency (IAEA)
fellowship on the Development and evaluation
of a loop-mediated isothermal amplification
method for diagnosis of Tropical Theileriosis
Research Center Borstel, Germany.
15 Aug –5 Nov 2011
Research Fellow at the BecA Hub. Project:
“Genotyping of Theileriaparva from cattle in
Southern Sudan to support control of ECF”
ILRI- Nairobi, Kenya.
20 Nov – 19 Dec2011 Research visit to Institute of Tropical Medicine
(ITM), Antwerp, Belgium, supported by
International Foundation for Science (IFS).
Project title “Molecular characterization of
Theileriaparva strains from South Sudan using
PCR-RLFP approach”
1 July – 30 Nov 2012 Research Fellow at the BecA Hub, Project:
“Genotyping of Theileria annulata from cattle in
Sudan” ILRI- Nairobi, Kenya.
21 Nov– 20 Dec 2013 CV Raman International Fellowship for African
Researchers, Department of Veterinary
Parasitology, College of Veterinary Science,
Guru Angad Dev Veterinary and Animal
Sciences University, Ludhiana-141004, India
1 March –15 April 2014 Research Fellow at the BecA Hub. “Genotyping
of Theileria annulata and Theileria parva from
cattle inEast Africa” ILRI- Nairobi, Kenya.
6
Postgraduate students
MSc
Student Title University Year of graduation
Role
Shareef A.M. A. Mulla Leishmania donovaniinfection in the domestic dog (Canisfamiliaris) in Eastern Sudan
Sudan Academy of Sciences
2011 Mentor
Emadeldinn A.O.
Abdelgadir Epidemiological Study on Babesiabigemina in cattle in Kassala State, Eastern Sudan
Sudan Academy of
Sciences 2012 Mentor
Ayman E.A. Elamin Study on Bovine Tuberculosis in Kassala State, Sudan
Sudan Academy of
Sciences 2012 Mentor
Mohamed A.M. A. Hayati
Prevalence of Ticks (Acari: Ixodidae) of Cattle and Theileriaannulata Infection in Gezira State, Sudan
University of
Khartoum 2015 Mentor
Sara A.H.M. Ali Seroprevalence of Theilerialestoquardiamong sheep and goats in Khartoum State, Sudan
University of
Khartoum 2016 Mentor
Nada A.R. Mohammed Prevalence of Theileriaannulata incattle and ticks in Medani area, Gezira State, Sudan using serological and molecular methods
University of Gezira 2016 Co-Supervisor
Ismail
AbdelrahimAbaker Molecular epidemiology of Theileriaannulata in cattle dairy farms in Nyala area, Southern Darfur State
University of
Khartoum 2016 Mentor
Tagwa M. Ibrahim Molecular epidemiology
ofTheilerialestoquardi from sheep
and goats in South Darfur State
University of
Khartoum 2017 Mentor
Awad I. A.Turky Molecular epidemiology of Theileriaparva in Eastern region of South Sudan
University of Nyala 2017 Co-Supervisor
Iman I. Ahmed Assessment of maternal immunity in newborn calves from dams naturally infected with Theileriaannulata and treated with Buparvaquone
Sudan University of
Science and
Technology
In progress Mentor
Habiba Thani Abd Elmumin Osman
Bovine Babesiosis in North Darfur State, Sudan
Sudan University of
Science and
Technology
In progress Co-Supervisor
PhD
Student Title University Year of
graduation
Role
Bothina B.M. Gaafar
Molecular Epidemiology of Anaplasmosis of Sheep and Goats in South Darfur State, Sudan
University of
Khartoum In progress Co-Supervisor
Hanan Dafalla
Mohamed
Molecular epidemiological study of
malignant ovine theileriosis in
Gezira state
Sudan Academy of
Sciences
In progress Mentor
Abubakar Ahmed Saaid Improvement of Efficacy of a local Sudan University of In progress Mentor
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TheileriaannulataAttenuated
Schizont Vaccine
Science and
Technology
Workshops/Conferences attended
1. International Workshop on Camel Research and Development
Formulating a Research Agenda for the Next Decade Wad Madani, Gezira
State, Sudan, December 9-12, 2002.
2. Third workshop on ticksandtick-bornediseases in the Sudan, Khartoum
(August 2003) resultingin a special issue of The Sudan Journal of Veterinary
Science and Animal Husbandry, Volume, 42, 2003
3. The 7th Scientific Conference, National Center for Research, December
2005, Friendship Hall, Khartoum, Sudan.
4. The 5th International Conference on Ticks and Tick-Borne Diseases
(TTP5), 29th August – 2nd September 2005 University of Neuchâtel,
Switzerland.
5. Conference on Attenuated vaccines for Animal Diseases, April, 2007
Borstel, Germany
6. 10th Biennial Conference of the Society for tropical Veterinary Medicine,
“One Health, One Medicine”, June 29th – July 3rd 2009, Lubeck, Germany.
7. East Coast fever Workshop, past, present and future prospective, South
Sudan, 8th – 10th December 2010, Bor, South Sudan.
8. TTP7: Seventh Ticks and Tick-borne Pathogens International
Conference. Zaragoza (Spain), 27 August – 3 September 2011.
9. Workshop on development of East Coast Fever control strategy for
South Sudan. 31th March – 1st April 2013 Organized by FAO, UN Campus,
Nairobi, Kenya
10. 6th Science Conclave, A Congregation of Nobel Laureates at Indian
Institute of Information Technology (IIIT), Allahabad, India, December 08-
14, 2013
11. Workshop on Characterization of Theileriaparva Strains Organized by
GALmed, 19- 20th January 2017,Best Western Hotel, Nairobi, Kenya
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12. Facilitated Proposal Development Workshop – ABCF alumni-led
Community of Practice on Ticks and Tick-borne Diseases. Organized by
BecA-ILRI Hub, 24th to 29th April 2017, ILRI campus, Nairobi, Kenya
13. Agricultural Research Connection (ARC) workshop, Organized by
BecA-ILRI Hub, Pride Inn Hotel, Nairobi, Kenya, 20-22 March 2018
Teaching experience (Undergraduate)
1. Teaching the courses of
immunoparasitologyandmolecularparasitologyforthe
4thClass,MedicalLaboratory Sciences Programme,Nile college.
2. Teaching the Basic andadvancedBioinformatics course forthe 5th
Class,Al Neelain University, MedicalLaboratory Sciences.
3. I served as externalexaminerforthe 3rdClass VeterinaryMedicine student,
University of Khartoum
Postgraduate examination board: I served as internalandexternalexaminerforthefollowing MSc and PhD
students: Student Degree-Year University My role
Adam Ahmed Adam Osman MSc-2014 Sudan Academy of Sciences Internalexaminer
HythamAbdallaOsman MSc-2014 University of Khartoum Externalexaminer
Osama Mohamed Mohamed MSc-2014 Sudan Academy of Sciences Internalexaminer
Ibrahim AwadHelibiElamin MSc-2015 University of Khartoum Externalexaminer
MarwaAbdelmoniemElmaki
Mohamed
MSc-2015 Sudan University of Science
and Technology
Externalexaminer
Mohamed Azhari Ibrahim
Eltom
MSc-2015 Sudan University of Science
and Technology
Externalexaminer
YassirAbakarBrima Ismael MSc-2017 University of Khartoum Externalexaminer
Mustafa Eltigani Mustafa
Yassin
PhD-2014 Al Neelain University Externalexaminer
Nagwa Mohamed-Ahmed
Mohamed Mohamed-Ahmed
PhD-2015 Sudan Academy of Sciences Internalexaminer
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Contribution to the scientific journals
- Assistant of scientific editor of the Sudan Journal of Veterinary Research
(Vol., 18, 2003).
- Secretary and member of the editorial board ofthe SudanJournal of
Veterinary Research since January 2014
- Reviewer for the following journals:
Acta Tropica
Acta Parasitologica
BioMed Research International
BMC Veterinary Research
Iranian Journal of Veterinary Research
Journal of Equine Veterinary Science
Journal of Medical Entomology
Journal of Parasitic Diseases
Journal of Parasitology and Vector Biology
Journal of Veterinary Medicine and Research
Parasitology
Tropical Animal Health and Production
Veterinary Medicine and Science
Veterinary Parasitology: Regional studies and Reports
Veterinary World
The Sudan Journal of Veterinary Science and Animal Husbandry
The Sudan Journal of Veterinary Research
10
Conference/workshops: papers/posters/presentations
1. Majid, A.A., Goraish, I.A. and Salih, D.A.(2002). Camel Research and
Development in the Sudan. In proceedings ofInternational
Workshop on Camel Research and Development Formulating a
Research Agenda for the Next Decade Wad Medani, Gezira State,
Sudan December 9-12, 2002.‘Paper’
2. Salih, D.A., Hassan, S., Bakheit, M., EL Hussein, A., Jongejan, F. and
Ahmed, J. (2005). Application of the Indirect TaSP Enzyme-linked
Immunosorbent Assay for Diagnosis of Theileriaannulata Infection in
Cattle. In: Proceedings of the 5th International Conference on Ticks
and Tick-Borne Pathogens, Neuchâtel, Switzerland, pp. 163-
166.‘Paper’
3. Ali, E.B.M., Salih, D.A. and Salman M.D. (2006). Determination of the
cut off for recently developed ELISA for sero-diagnosis of
Theileriaannulata. In: Proceedings of the International Symposium on
Veterinary Epidemiology and Economics “ISVEE”.‘Paper’
4. Sharieff, O.E., El Hussein, A.M., Hassan, S.M. and Salih,
D.A.(2006).Attenuation of Sudanese schizont-infected cell lines of
Theileriaannulata. In proceedings of the 7th Scientific Conference,
National Center for Research, Friendship Hall, Khartoum, Sudan 13-
15 December 2005. ‘Presentation’
5.Salih, D.A.,(2008).Comparison between Reverse Line Blot and Enzyme-
linked Immunosorbent Assay inDiagnosis of Major Tick-borne
Diseases of Cattle in Southern Sudan 10thBiennial Conference of
the Society for Tropical Veterinary MedicineOne Health, One
Medicine: Building Bridges to Face the Challenge of Emerging and
11
Zoonotic Diseases. June 29th- July 3rd, 2009, Lubeck, Germany.
‘Poster’
6. Salih, D.A.,Geysen, D., Seitzer, U.AhmcdJabbar, A. and Hussein,
A.M.(2011).Molecular characterization of different strains of
Theileriaparvafrom Southern Sudan. InternationalConference on
Ticks and Tick-borne Pathogens - TTP7, Zaragoza, Spain, August
28th – September 2nd 2011. ‘Paper’
7. Salih, D.A. (2013).Molecular epidemiology of tick-borne diseases with
special referenceto East Coast fever in Central Equatoria State,
South Sudan. Workshop on development of East Coast Fever
control strategy for South Sudan. 31stMarch – 1stApril 2013
Organized by FAO, UN Campus, Nairobi, Kenya. ‘Presentation’
8. Salih, D.A., Taha, K.M., Hussein, M.O., Enan, K.A., Sharma, A., Singla,
L.D., El Hussein, A.M.(2016).Tams-1 Sequence diversity in
Theileriaannulata: The effect of vaccination with live vaccine on
parasite diversity. Joint AITVM/STVM Conference, Berlin Free
University, Berlin, Germany, September 4-8, 2016. ‘Poster’
9. Hayati, M.A., Hassan, S.M., Hussein, M.O., El Haj, L.M., Abdel Gadir,
S.O., Osman, M.M., Ahmed, R.E., El Hussein, A.M. Salih,
D.A.(2016).Prevalence of ticks (Acari: Ixodidae) and
Theileriaannulata infection in cattle in Gezira State, Sudan. Joint
AITVM/STVM Conference, Berlin Free University, Berlin,
Germany, September 4-8, 2016.‘Poster’
10. Salih, D.A.(2017).Genetic and antigenic diversity and population
structure of Theileriaparva in South SudanEast Coast fever (ECF)
Workshop and Training on genetic characterization of Theileriaparva
field samples. Best Western Hotel, Nairobi, Kenya.19th&20th January
2017.‘Presentation’
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Books
Hassan S.M. and Salih D.A.(2008). Bibliography with abstracts ticks and
tick-borne diseases in the Sudan (1908-2007). National Library
Cataloging.First Edition. Publisher: Central Laboratory, Ministry of Science
and Technology.pp. 176. ISBN: 978–99942–906–9-1 Deposit No. 1/2009
Hassan S.M. and Salih D.A.(2016). Bibliography with abstracts ticks and
tick-borne diseases in the Sudan (1908-2016). National Library
Cataloging.Second Edition. Publisher: Animal Resources Research
Corporation, Ministry of Animal Resources. pp. 173. ISBN: 978–99942–4–
673-1 Deposit No. 2016/881
Author ID
Loop profile: 135146
Scopus Author ID: 7801387348
ORCID: https://orcid.org/0000-0002-4278-9754
13
List of publications: (Peer reviewed Journals)
1. Awad, H., Al-Hamidhi, S., El Hussein, A.R., Taha, K.M., Salih, D.A.,
Weir, W. and Babiker, H. (2018).Theilerialestoquardi in Sudan is highly
diverse and genetically distinct from that in Oman.Infection, Genetics
and Evolution,62: 46–52
2. Salih, D.A.,Mwacharo, J.M., Pelle, R., Njahira, M.N., Odongo, D.O.,
Mbole-Kariuki, M.N., Marcellino, W.L., Malak, A.K., Kiara, H., El
Hussein, A.M., Bishop, R.P. and Skilton, R.A. (2018). Genetic
diversity and population structure of Theileriaparva in South Sudan.
Ticks and Tick-Borne Diseases, https://doi.org/10.1016/j.ttbdis.2018.03.002
3. Yin, F., Liu, Z., Liu, J., Liu, A., Salih, D.A.,Li, Y., Liu, G., Luo, J., Guan,
G. and Yin, H. (2018).Population genetic analysis of Theileriaannulata
from six geographical regions in China, determined on the basis of
micro- and mini-satellite markers. Frontiers in
Genetics,9:50.https://doi.org/10.3389/fgene.2018.00050
4. Mohammed-Ahmed, G.M., Hassan, S.M., El Hussein, A.M. and Salih,
D.A.(2018).Molecular, serological and parasitological survey of
Theileriaannulata in North Kordofan State, Sudan. Veterinary
Parasitology: Regional Studies and reports, 13: 24-
29https://doi.org/10. 1016/j.vprsr.2018.03.006
5. Sara A. Hassan, Hassan, S.M., Hussein, M.O., Limia M. El Haj, Manal M.
Osman, Rawia E. Ahmed, Selma O. Abdel Gadir, El Hussein, A.M.
and Salih, D.A.(2018). Prevalence of Theilerialestoquardi Antibodies
and Ticks (Acari: Ixodidae) in Sheep and Goats in Khartoum State,
Sudan.Sudan Journal of Veterinary Research,33: 31-37.
6.Salih, D.A.,Pelle, R., Mwacharo, J.M., Njahira, M.N.,Marcellino, W.L.,
Kiara, H., Malak, A.K., El Hussein, A.M., Bishop, R. andSkilton, R.A.
(2017).Genes encoding two Theileriaparva antigens recognized by
CD8+T-cells exhibit sequence diversity in South Sudanese cattle
14
populations but the majority of alleles are similar to the Muguga
component of the live vaccine cocktail. PLoS ONE,12(2):
e0171426.doi: 10.1371/journal.pone.0171426
7. Ali, A.M., Salih, D.A.,Njahira, M.N., Hassan, S.K., El Hussein, A.M.,
Liu, Z., Yin, H., Pelle, R. and Skilton, R.A. (2017).Genotyping of
Theilerialestoquardi from sheep and goats in Sudan to support control
of malignant ovine theileriosis.Veterinary Parasitology,239:7-14.
8. Marcellino, W.L., Salih, D.A.,Njahira, M.N., Ndiwa, N., Araba, A., El
Hussein, A.M., Seitzer, U., Ahmed, J.S., Bishop, R.P., Skilton, R.A.
(2017). The emergence of Theileriaparva in Jonglei State, South Sudan:
Confirmation using molecular and serological diagnostic tools.
Transboundary and Emerging Diseases,64(4):1229-1235
doi:10.1111/tbed.12495
9. Abaker, I.A., Salih, D.A., El Haj, L.M., Ahmed, R.E., Osman, M.M., Ali,
A.M.(2017). Prevalence of Theileriaannulata in dairy cattle in Nyala,
South Darfur State, Sudan, Veterinary World,10(12):1475-1480.
10. Sharieff, O.E., Hassan, S.M., Salih, D.A. and El Hussein, A.M. (2017).
Attenuation of two field isolates of Theileriaannulata and their potential
as vaccine candidates against tropical theileriosis in the Sudan.
International Journal of Scientific Research in Science,
Engineering and Technology,(3)1: 54-58.
11. Elkhazeen, K.A., Yaji, R.A., Salih, D.A.,Omer, R.A. and Abdelgadir,
A.E. (2017).Prevalence and risk factors associated with bovine
trypanosomosisusing conventional methods and polymerase chain
reaction (PCR) in the Blue NileState, Sudan. Journal of Applied
Science and Research,5 (2):12-23.
12. Osman, T.M., Ali, A.M., Hussein, M.O., El Ghali, A. andSalih, D.A.
(2017).Investigation on Theilerialestoquardiinfection among sheep and
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goats in Nyala, South Darfur State, Sudan. Insights in Veterinary
Science,1: 017-023.
13. Mohammed, A.A., Sharma, A., Saied, M.A.M., Osman, O.H., Al-Balowi,
M.H., Salih, D.A. and Singla, L.D. (2017).Lack of evidence for
infection of camels with tick-borne diseases in Riyadh region, Saudi
Arabia.Sudan Journal of Veterinary Research,32: 39-40.
14. Rukambile, E., Machuka, E., Njahira, M., Kyalo, M., Skilton, R.,Mwega,
E., Chota, A., Mathias, M., Sallu, R., Salih, D.(2016).Population
genetic analysis of Theileria parva isolated in cattle and buffaloes in
Tanzania using minisatellite and microsatellite markers.Veterinary
Parasitology,224: 20–26.
15. Sayed, M., Hassan, S., ElHussein, A., Altaib, N., Faisal, S., Shikh, M.,
Salih, D.and Abdel Rahman, M.(2016). Pathogenicity of
Ehrlichiaruminantiumdifferent local stocks in the Sudan and estimation
of Amblyommatick victorialcapacity as invective dose by Real-time
PCR. Butana Journal of Applied Science,1: 1-12
16. Hussien, M.O., Taha, K.M.,Enan, K., Salih, D.A.and El Hussein, A.M.
(2016).Immunization with live attenuated schizonts against tropical
theileriosis (Theileriaannulata infection) increases antibody titers in
chronically infected cattle in Sudan. Austin Journal of Vaccines
&Immunotherapeutics,3(1): 1009.
17. Mulla, S., Osman, A.Y., Hussien, M.O.,Salih, D.A.and El Hussein,
A.M.(2016).Leishmaniadonovani infection in the domestic dog
(canisfamiliaris) in Eastern Sudan.International Journal of Biological
& Pharmaceutical Research, 7(2):109-115.
18.Salih, D.A., El Hussein, A.M. and Singla, L.D. (2015). Diagnostic
approaches for tick-borne parasitic diseases in livestock: from basics
to molecular concepts. Journal of Veterinary Medicine and
Animal Health,6 (7): 45-56.
16
19.Salih, D.A., El Hussein, A.M., Marcellino, W.L., Berkvens, D. and
Geysen, D. (2015). Molecular characterization of Theileriaparva strains
from South Sudan using PCR-RLFP approach on antigen genes.
Archives of Razi Institute,70 (1):13-20.
20. El Imam, A.H., Hassan, S.M., Gameel, A.A., El Hussein, A.M., Taha,
K.M. and Salih, D.A.(2015).Variation in susceptibility of three
Sudanese sheep ecotypes to natural infection with Theilerialestoquardi.
SmallRuminantResearch, 124: 105-111.
21. Bothina B.M. Gaafar, Hassan, S.M., Limia M. El Hag and Salih,
D.A.(2015).Prevalence of tropical theileriosis in Nyala dairy farms in
South Darfur State, Sudan. Sudan Journal of Veterinary Research,30:
7-12.
22. Omer, E.A., Mohammed, A.S., Hassan, S.M. and Salih, D.A.(2015).
Prevalence of Babesiabigemina and ticks infestation of cattle in Kassala
State, Eastern Sudan. Sudan Journal of Veterinary Research,30: 13-18.
23. Nyoap, S.S.M., Majok, A.A., and Salih, D.A.(2015). A survey of ticks
and East Coast fever among cattle in Fangak County, Jonglei State,
South Sudan. Journal of Veterinary Medicine and Animal
Health,7(7): 249-256.
24. Guma, E.I., Hussien, M., Salih, D.A., Salim, B. and Hassan, S.M.
(2015). Prevalence of ticks (Acari: Ixodidae) and Theileriaannulata
antibodies in White Nile State, Sudan. Journal of Advanced
Veterinary and Animal Research,2(1): 69-73.
25. Mwega, E., Salih, D.,Njahira, M., Rukambile, E., Skilton, R. and
Gwakisa, P.(2014). Genetic and antigenic diversity of Theileriaparva in
cattle in Eastern and Southern zones of Tanzania. A study to support
control of East Coast fever. Parasitology,142(5):698-705
17
26. Liu, Z., Li, Y., Salih, D.A., Luo, J., Ahmed, S.J., Seitzer, U. and Yin, H.
(2014). Validation and application of TuIP ELISA for detection of
ovine theileriosis of small ruminants in China. Veterinary Parasitology,
204(3-4):139-145.
27. Vaumourin, E., Vourc’h, G., Telfer, S., Lambin, X., Salih, D.A., Seitzer,
U., Morand, S., Charbonnel, N., Vayssier-Taussat, M., Gasqui. P.
(2014). To be or not to be associated: power study of four statistical
modeling approaches to identify pathogen associations in cross-
sectional studies. Frontiers in Cellular and Infection Microbiology,
4:62.doi: 10.3389/fcimb.2014.00062
28. Ayman, E.A., Salih, D.A.,Gumaa, M.M., Omer, M.M. Abbas, F. and
Ahmed, A.M.(2014). Prevalence of Bovine tuberculosis in Kassala
State, Eastern the Sudan. International Journal of Advanced
Scientific and Technical Research, 4(1): 238-249.
29.Salih, D.A., Ali, A.M., Hassan, S.M., Mukhtar, M.M., El Hussein, A.M.,
Seitzer, U. and Ahmed, J. (2013). Tropical theileriosis
(Theileriaannulata infection of cattle) in Khartoum State, Sudan: Risk
factors. Sudan Journal of Veterinary Research, 28:51-52.
30. Renneker, S., Abdo, J., Salih, D.A.,Karagenç, T., Bilgiç, H., Torina, A.,
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32. Elfahal, A.M., Hussien, M.O., Enan, K.A., Taha, K.M., Salih,
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Investigation of caprine arthritis-encephalitis virus in Sudan using
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33. Taha, K.M., Salih, D.A., Ali, A.M., Omer, R.A. and El Hussein, A.M.
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34.Salih, D.A., Ali, A.M., Liu, Z., Bakheit, M.A., Taha, K.M.,
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35. Marcellino, W.L., Salih, D.A.,Julla, I.I. and El Hussein A.M. (2012).
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36. El Hussein, A.M., Hassan, S.M. and Salih, D.A.(2012). Current
situation of tropical theileriosis in the Sudan. Parasitology
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37. Hussien, M.O., El Fahal, A.M., Enan, K.A., Taha, K.M., Mohammed,
M.S., Salih, D.A.,Mohammadain, S.I., Saeed, A.A. and El Hussein,
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39. Marcellino, W.L., Salih, D.A.,Julla, I.I. and El Hussein, A.M. (2011).
Economic impact of East Coast fever in Central Equatorial State of
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40. Gumaa, M.M., Abusalab, S., Omer, M.M., Salih, D.A., Mulla, S.A.,
Omer, E.A. and Ahmed, A.M. (2011). A two-year study on bovine
trypanosomosis in Kassala State, Eastern Sudan (2007-2008).
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41. Taha, K.M., Salih, D.A., Ahmed, B.M., Enan, K.A., Ali, A.M., El
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Comparison between reverse line blot and enzyme-linked
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cattle in Southern Sudan. Transboundary and Emerging Diseases,
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43. Müller, H., Selek, N., Liu, Z., Salih, D.A.,Karagenc, T., Beyer, D.,
Kullmann, B., Ahmed, J.S. and Seitzer, U. (2010).Development of a
loop-mediated isothermal amplification (LAMP) assay for rapid
diagnosis of Babesiacanis infections. Transboundary and Emerging
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44. Liu, Z., Wang, Z., Yin, H., Luo, J., Zhang, B., Kullmann, B., Abdo, J.,
Salih, D.A.,Ahmed, J. and Seitzer, U. (2010).Identification of
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45. Al-Saeed, A.T.M., Omer, L.T., Abdo, J., Habibi, G., Salih, D.A., Seitzer,
U., Ahmed, J. (2010). Epidemiological studies on tropical theileriosis
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46.Salih, D.A., Abdel Rahman, M.B., Mohammed, A.S., Ahmed, R., Kamal,
S. and El Hussein A.M. (2009). Seroprevalence of tick-borne diseases
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H., Viljoen, G., Seitzer, U. and Ahmed, J.S. (2008). Development and
evaluation of a loop-mediated isothermal amplification method for
diagnosis of tropical theileriosis. Transboundary and Emerging
Diseases, 55: 238–243.
48.Salih, D.A., Hassan, S.M., Julla, I.I., Kyule, M.N., Zessin, K-H. and El
Hussein, A.M. (2008). Distribution and application of ELISA for the
seroprevalence of tick-borne diseases in Central Equatoria State,
Sudan. Transboundary and Emerging Diseases, 55: 257–262.
49. Ali, A.M., Beyer, D., Bakheit, M.A., Kullmann, B., Salih, D.A., Ahmed,
J.S. and Seitzer, U. (2008). Influence of subculturing on gene
expression in a Theilerialestoquardi-infected cell line. Vaccine,26S:
G17–G23.
50. Ali, A.M., Salih, D.A., Bakheit, M.A., El Hussein, A.M., Hassan, S.M.,
Mukhtar, M.M., Ahmed, J.S. and Seitzer, U. (2008).
Comparison of the molecular structure of the TaSP gene of Theileria
annulata from Sudanese isolates. Annals of the New York
Academy of Sciences, 1149: 218–220.
51. Liu, Z., Hou, J., Bakheit, M.A., Salih, D.A.,Luo, J., Yin, H., Ahmed, J.S.
and Seitzer, U. (2008). Development of loop-mediated isothermal
21
amplification (LAMP) assay for rapid diagnosis of ovine theileriosis in
China. Parasitology Research, 103(6): 1407-1412.
52. Ahmed, J.S., Glass, E.J., Salih, D.A. and Seitzer, U. (2008). Innate
immunity to tropical theileriosis: Review. Innate Immunity, 14 (1):
5-12.
53.Salih, D.A.,Julla, I.I., Hassan, S.M., El Hussein, A.M. and Jongejan, F.
(2008). Preliminary survey of ticks (Acari: Ixodidae) on cattle in
Central Equatoria State, Southern Sudan. Onderstepoort Journal of
Veterinary Research, 75: 47-53.
54.Salih, D.A., El Hussein, A.M., Seitzer, U. and Ahmed, J.S. (2007).
Epidemiological studies on tick-borne diseases of cattle in Central
Equatoria State, Southern Sudan. Parasitology Research, 101(4):
1035-1044.
55.Salih, D.A., El Hussein, A.M., Kyule, M.N., Zessin, K.H., Ahmed, J.S.
and Seitzer, U. (2007). Determination of potential risk factors
associated with Theileriaannulata and Theileriaparva infections of cattle
in the Sudan. Parasitology Research, 101(5): 1285-1288.
56.Salih, D.A., Hassan, S.M. and El Hussein, A.M. (2007). Comparisons
among two serological tests and microscopic examination for the
detection of Theileriaannulata in cattle in northern Sudan. Preventive
Veterinary Medicine, 81: 323-326.
57. Seitzer, U., Bakheit, M.A., Salih, D.A., Ali, A., Haller, D., Yin, H.,
Schnittger, L. and Ahmed, J. (2007).From molecule to diagnostic
tool: Theileria annulata surface protein TaSP. Parasitology
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58. Omer, M.M., Aziz, A.A.A. and Salih, D.A.(2006). Rabies in a camel
(Camelus dromedarius) in Kassala State - Eastern Sudan. The Sudan
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59.Salih, D.A., Aziz, A.A.A. and Omer, M.M. (2005). Diseases of livestock
in Kassala State, Eastern Sudan: A Ten-year Retrospective Study.
The Sudan Journal of Veterinary Science and Animal
Husbandry, 44: 136-150.
60.Salih, D.A., Ahmed, J.S., Bakheit, M.A., Ali, E.B., El Hussein, A.M.,
Hassan, S.M., Shariff, O.E., Fadl, M. and Jongejan, F. (2005).
Validation of the Indirect TaSP Enzyme-linked Immunosorbent
Assay for Diagnosis of Theileria annulata Infection in Cattle.
Parasitology Research, 97(4): 302-308.
61.Salih, D.A.,Sharieff, O.E., Lazarus, A.G., Hassan, S.M. and El Hussein,
A.M. (2005). Natural infection rates and transmission of
Theileriaannulata by Hyalommaanatolicumanatolicum ticks in the Sudan.
Onderstepoort Journal of Veterinary Research, 72: 303-307.
62. Bakheit, M.A., Schnittger, L., Salih, D.A.,Boguslawski, K., Beyer, D.,
Fadl, M. and Ahmed, J.S. (2004). Application of the recombinant
Theileriaannulata surface protein in an indirect ELISA for the diagnosis
of tropical theileriosis. Parasitology Research, 92: 299 – 302.
63.Salih, D.A., Hassan, S.M., El Hussein, A.M. and Jongejan, F. (2004).
Preliminary survey of ticks (Acari: Ixodidae) on cattle in northern
Sudan. Onderstepoort Journal of Veterinary Research, 71: 319-326.
64. Taha, K.M., El Hussein, A.M., Abdalla, H.S. and Salih,
D.A.(2003).Theilerialestoquardi infection in goats in River Nile State:
Comparison of serology and blood smears. The Sudan Journal of
Veterinary Science and Animal Husbandry, 42: (1&2): 197- 206.
65. Sharieff, O.E., El Hussein, A.M., Hassan, S.M., Salma, K.,Salih, D.A.
and Majid, A.A. (2003). Establishment of Theileria cell culture
system in the Sudan: A minireview. The Sudan Journal of
Veterinary Science and Animal Husbandry, 42: (1&2): 147- 158.
23
66. Mohammad, A.A. and Salih, D.A.(2003).Theileria infection in a local
goat in Red Sea State, Eastern Sudan. A case report. The Sudan
Journal of Veterinary Science and Animal Husbandry, 42: (1&2):
176 – 180.
67. Abdel Rahman, M.B., Salih, D.A., El Hussein, A.M., Hassan, S.M.,
Abdel Rahim, A.I., Hamid, M.E. and Majid, A.A. (2003). Diagnosis
of tick-borne diseases: II. Diagnosis of Cowdriosis. The Sudan
Journal of Veterinary Science and Animal Husbandry, 42: (1&2):
228 – 238.
68. Ahmed, B.M., El Hussein, A.M., El Ghali, A., and Salih, D.A.(2003).
Some studies on the epidemiology of ovine theileriosis in River Nile
State, northern Sudan. Journal of Animal and Veterinary
Advances, 2(12): 681-685.
69.Salih, D.A., El Hussein, A.M., Taha, K.M. and Hayat, M. (2003). Survey
of Theileria lestoquardi antibodies among Sudanese sheep. Veterinary
Parasitology,111: 361-367.
70.Salih, D.A., El Hussein, A.M., Majid, A.M. and Hassan, S.M. (2003).
Molecular techniques in the diagnosis of tick-borne diseases in the
Sudan: Overview. The Sudan Journal of Veterinary Science and
Animal Husbandry,42: (1&2): 207-218.
71.Salih, D.A., El Hussein, A.M., Hassan, S.M., Abdel Rahman, M.B.,
Abdel Rahim, A.I., Hamid, M.E. and Majid, A.M. (2003). Diagnosis
of tick-borne diseases: I. Diagnosis of Theileriosis. The Sudan
Journal of Veterinary Science and Animal Husbandry, 42: (1&2):
219-227.
24
Manuscripts in Preparation
1. Elhaj, M.T., Taha, K.M., El Ghali, A., Hassan, S.M., Salih, D.A.,Nijhof,
A., Ahmed, J.S., Clausen, P.-H. and El Hussein, A.M.Distribution of
Ixodid Ticks Infesting Cattle in Northern State, Sudan.
2. Hayati, M.A.M., Hassan, S.M.,Salih, D.A. Prevalence of Ticks (Acari:
Ixodidae) and TheileriaannulataInfection of Cattle in Central Gezira
State, Sudan.
3. Nanteza, A., Obara, I., Kasaija, P., Mwega, E., Kabi, F., Salih,
D.A.,Njahira, M., Njuguna, J., Odongo, D., Bishop, R.P., Skilton,
R.A., Ahmed, J., Clausen, P.-H., Lubega, G.W.Antigenic and VNTR
variation in Theileriaparva parasites in the Lake Mburo region in
southwestern Uganda: Implications for the deployment of a live
vaccine.
4. Gharbi, M., Elati, K., Al Hosary, A., Ayadi, O., Salih, D.A.,El Hussein,
A.M., Khammassi, M., Hassan, S.M. and Darghouth M.A.
Epidemiological status of tropical theileriosis (Theileriaannulata
infection) in Africa.
5. Al Shaekh, A.I., Mohammed, A.A., El Ghali, A.and Salih,
D.A.Distribution of Ixodid Ticks on cattle in Great Bahr El Ghazal
states, South Sudan.
6. Al Shaekh, A.I., Mohammed, A.A. and Salih, D.A. Molecular
Epidemiology of East Coast Fever in Great Bahr El Ghazal states,
South Sudan.
