CURRICULUM VITAE

STEPHANIE GAIL BURTON

BSc (Hons), Grad CE, MSc, PhD

June 2009

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PRESENT POSITION: Director of Postgraduate Studies Leader: Biocatalysis and Technical Biology Research Group

Department of Research, Technology Innovation and Partnerships Cape Peninsula University of Technology Bellville 7353 Cape Town South Africa CONTACT DETAILS: Phone: + 27 21 953 8455 or 8499 Fax: +27 21 953 8456 E-mail: burtons@cput.ac.za

Mobile: + 27 82 555 8663 ACADEMIC QUALIFICATIONS:

BSc(Hons), University of Rhodesia, 1976 Graduate Certificate in Education, University of Rhodesia, 1977 MSc (with Distinction) in Organic Chemistry, Rhodes University, 1990

PhD in Biochemistry, Rhodes University, 1994 FRD RATING : B3 Awarded 2006 PERSONAL DETAILS:

Date of birth: 30/8/1955 Place of birth: Harare, Zimbabwe Married to Professor Don A Cowan Two adult children South African citizen (ID 5508300242085)

CAREER AND WORK EXPERIENCE:

School education: Salisbury (now Harare) at Salisbury Girls' High School 1974 - 1976: Attended University of Rhodesia (Zimbabwe), obtaining a BSc degree with Honours. 1977: Graduate Certificate of Education, University of Rhodesia-Zimbabwe. 1989: Attained MSc. degree in Organic Chemistry, with Distinction 1990-1993: Studied for a PhD in Biochemistry (Enzyme Biotechnology) 1994: Awarded PhD (Rhodes) 1991-1995: Junior Lecturer in the Department of Biochemistry and Microbiology, Rhodes

University 1996-1998: Lecturer in the Department of Biochemistry and Microbiology, Rhodes University

1998-2001: Senior Lecturer in the Department of Biochemistry and Microbiology, Rhodes University.

2001–2002: Senior Lecturer in Chemical Engineering, University of Cape Town 2002-2004: Associate Professor in Chemical Engineering, University of Cape Town 2005-2008: Full Professor in Chemical Engineering, University of Cape Town 2009- present: Director: Postgraduate Studies, Cape Peninsula University of Technology. AWARDS: Round Table Prize for best First year student in Chemistry, 1974

Rhodesian Government Scholarship for University study, 1974 - 1976

Rhodesian Government Bursary for post-graduate study, 1977

Cape Education Department Merit award for excellence in teaching, 1986

FRD bursary for Masters degree, 1989

AECI bursary for PhD studies, 1990-1993

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FRD Science Liason Travel grant (Zimbabwe), 1997 FRD Science Liason Travel grant (USA) 1998 Collano Prize for Innovative Science (Switzerland) 2004

South African Institute for Chemical Engineering (SAIChE) Chemical Technology Prize, 2004 SAIChE Chemical Technology Prize, SA, 2005

UNIVERSITY TEACHING EXPERIENCE AND RESPONSIBILITIES 1991 - 1995:

Undergraduate lecture courses and practical classes in Biochemistry Honours lecture courses in Biochemistry and Biotechnology Supervision of Postgraduate students in the Department of Biochemistry and Microbiology, and Department of Chemistry, Rhodes University Coordination of the Honours Course in Biotechnology, Department of Biochemistry and Microbiology, Rhodes University.

1996 – 2001: Undergraduate lecture courses in Biochemistry and Honours courses in Biochemistry and Biotechnology Supervision of Postgraduate students in the Department of Biochemistry and Microbiology, Rhodes University Coordination of the Honours Course in Biotechnology, Department of Biochemistry and Microbiology, Rhodes University Initiation and Coordination of the Introductory Course for Honours students in Biochemistry, Biotechnology and Microbiology, Rhodes University

Initiation and organisation of the departmental Honours Introductory Excursion. Administration of the Gold Fields Biotechnology Centre Laboratory, Department of Biochemistry and Microbiology, Rhodes University (1996 - 1997)

Coordination of the Departmental Research Seminar Programmes (1996 - 1998). Departmental representative - Environmental Science at Rhodes (1998). Departmental representative for SASOL Festival of Science, Engineering and Technology (SciFest) ‘98 and ‘99.

Initiation and Coordination of Quality Assurance (Academic) Programme in the Department of Biochemistry, Microbiology and Biotechnology, Rhodes University (1998 - present). Initiation and Coordination of the Curriculum Development Programme in the Department of Biochemistry and Microbiology, Rhodes University (1998 - present). Courses attended: Rhodes University Academic Development Programme Professional Development Programmes:

(1) Evaluation: Balancing quality assurance with quality improvement issues (2) Assessment for Outcomes-based Education programmes. (3) Formulating Learning Outcomes

Workshop on Tutor Training held at Stellenbosch University, Jan 1999 2001 – 2008: Chemical Engineering Department, UCT

Undergraduate courses: CHE 2031F Mass and Energy Balances (2000 -2004), CHE 2035S Thermodynamics (2002-2003) CHE 2033W Chemical Engineering Lab (2000 - present) CHE 3035S Bioprocess Technology I (2001 - present) CHE 4048F Business, Engineering and Society (2005-2008)

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Masters level courses: CHE 5052W Molecular Biology and Biocatalysis (2001 – 2008)

CHE 5055Z Research Methodology and Communication (2005 - 2008) END 5049Z Research Methodology and Communication (2007 - 2008)

Molecular and Cell Biology Department, UCT (2001 – 2008) MCB 310S Module: Biotransformations (Third year) Department of Biotechnology, Univerity of the Western Cape (2002 - 2005) BTY 333 Industrial Biotechnology EXTERNAL EXAMINING APPOINTMENTS: Department of Biochemistry, University of Natal (Biochemistry, third year courses (1997 - 2002) Masters and PhDs: Department of Biochemistry, University of Natal (1999). Department of Microbiology and Biochemistry, University of the Orange Free State (1997, 1999). Department of Microbiology and Biochemistry, University of the Stellenbosch (2003). Department of Microbiology, M L Sultan Technikon (2003) Department of Chemical Engineering, University of Sydney (2004) Department of Microbiology, University of the Free State, (2005) Department of Biotechnology and Biomolecular Sciences, University of New South Wales (2006) Department of Chemical Engineering, University of KZN (2007) Department of Biotechnology, Cape Peninsula University of Technology (2007) Department of Biochemistry, Microbiology and Biotechology. Rhodes University (2008) Department of Microbiology, University of Waikato, New Zealand (2008) Faculty of Life Sciences, Swinburne University of Technology, Australia (2008) Department of Chemical Engineering, Cape Peninsula University of Technology (2008) Department of Biochemistry, Rhodes University (2009) REVIEWING FOR INTERNATIONAL JOURNALS: Applied Microbiology and Biotechnology Applied and Environmental Microbiology Biocatalysis and Biotransformations Biochemical Journal Biotechnology and Bioengineering Biotechnology Progress Enzyme and Microbial Technology Letters in Applied Microbiology

J. Agricultural and Food Chemistry J. Applied Microbiology J. Environmental Management

J. Molecular Catalysis B: Enzymatic (and Member of Editorial Board) Journal of Thermal Analysis and Calorimetry (Special Edition, 1999)

Plant Physiology Tetrahedron Letters

Talanta Water SA EDITORIAL BOARDS:

J. Molecular Catalysis B: Enzymatic Biocatalysis and Biotransformations Enzyme and Microbial Technology International Advisory Board

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ADMINISTRATION AND POSITIONS OF RESPONSIBILITY IN THE UNIVERSITY Current: Director: Postgraduate Studies Member of Senate

Member of Senate Committees: Higher Degrees Committee Academic Planning Library Committee

At University of Cape Town (2001 – 2008) Vice-Chair, UCT Senate Academic Planning Committee Member, Academic Staff Development Committee Member of UCT Senate Member of UCT Transformation Forum

Seconded by DVC Cheryl de la Rey to formulate and propose UCT a plan for a UCT Postgraduate School; The project included visits to several leading international Graduate Schools and extended to participation in seminars and work shops on the nature of academic research, particularly for PhD students, in collaboration with the Centre for Higher Education Development. Member of the Selection Committee for the position of Dean, Faculty of Engineering and the Built Environment (2007)

Convenor, Faculty Employment Equity Working Group and

Faculty Transformation Forum (2005-2007)

Project leader, EBE Faculty Carnegie Project Member, UCT Carnegie Project team (2005-2007) Member, EBE Faculty Post-Graduate Planning and Administration Committee Member, EBE Faculty Working Group on Ethics Member, EBE Faculty Human Resources Committee (2005-2007) UCT Biotechnology Thrust Committee (2000 – 2004) UCT EBE Faculty Research Committee (2002 – 2005) At Rhodes University (1997 – 2001) Rhodes University Student Disciplinary Board (1997, 1998).

Member of Conference Organising committees:

4th Congress of South African Biochemistry Society (January 1997), Biotech SA ‘97 (January 1997). BIO Y2K (Combined Biotech, Biochem and Microbiol. conference) (January 2000)

Faculty of Science Elected Representative on the Rhodes University Committee for Quality Assurance (from September 1998 - 2001).

Member of the Rhodes University Planning and Resource Committee (1999). Initiation and Management of the Department of Biochemistry and Microbiology programmes in Curriculum Development and Quality Assurance Public lecture at invitation of the Physics Department, Rhodes University: “The new national Education Policy- what it means for Rhodes lecturers.” September 1998.

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Workshop on Curriculum Development and Quality Assurance run for staff of the Department of Biochemistry and Microbiology, University of Fort Hare, August 1999

Executive Committee member for the Graduate School of Molecular Sciences, Rhodes University (from November 1998 - 2001).

ACTIVITIES AT NATIONAL LEVEL Current:

Vice Chairman: National Science and Technology Forum (NSTF) Executive Committee

Chairman: NSTF Science, Engineering and Technological Societies and Allied Professions Group (NSTF-SETAG) Executive Committee University representative: DST Audit of the National Biotechnology Stategy

University representative: Cape Biotech Trust Academic Forum Vice-President, Federation of African Societies of Biochemistry and Molecular Biology

Honorary Treasurer, South African Society of Biochemistry and Molecular Biology (SASBMB) (2004 - present) Member and National Voting Representative of the South African National Council for the International Union of Biochemistry and Molecular Biology (IUBMB, 2009)

Recent and past:

Chairman, Biotechnology Advisory Committee, Cape Peninsula University of Technology

Member of Cape Biotech Trust Advisory Committee and Strategy Committee (2003-2007) Assessor for National Research Foundation Focus Area Economic Growth and International Competitiveness (2002 – present) President of the South African Society for Biochemistry and Molecular Biology (SASBMB), (2001 - 2004)

Nominated for SABC2/Checkers Shoprite Woman in Science of the Year (2005) Invited attendee at the Consultative Conference of Women in Science, Engineering and Technology, at the invitation of the DST initiative South African Reference Group for Women in Science and Technology (SET4W), advisers to the Minster of Science on matters pertaining to women in SET

National Advisory Committee for SASOL Festival of Science, Engineering and Technology (Scifest ‘98 and SciFest ‘99)

Assessor for National Research Foundation Institutional Research Development Programme project proposals (1997 – 2001) and Focus Area Economic Growth and International Competitiveness proposals (2002 – present)

FRD Capital Equipment Panel for Major Capital Equipment (1998 -2000)

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Coordinator and Spokesman, Foundation for Research Development (FRD) Task Group for Biotechnology (Biochemical Processes, Competitive Industries) (1997 - 2000).

Member of NRF Network of Expertise in Biotransformations (1997-2001). Secretary and Conference Organiser, Water Institute of South Africa - Membrane Technology

Division (WISA-MTD) (1997 - 1999)

Invited chair: Department of Trade and Industry (DTI) conference on Commercialisation of Biotechnology, March 1999

Invited co-chair: FRD National Advisory Committee on Biotechnology meeting, May 1999

Member and National Voting Representative of the South African National Committee for the

International Union of Biochemistry and Molecular Biology (IUBMB, 1998 - 2003)

Member of the Council of the South African Society for Biochemistry and Molecular Biology (SASBMB), (1998 - 2001)

Co-leader of a South African Microbial Biodiversity Initiative in collaboration with the NRF and

Professor D A Cowan, (formerly University College London, now UWC) and Convenor of a National workshop to establish a National Network on Expertise on Microbial Biodiversity (held October 2000). Collaborator with Professor D A Cowan and Professor S Harrison in a Proposal for a Centre of Applied Microbiology and Genomics to be established at UWC.

Member of the Voluntary Advisory Committee of the Chemical and Allied Industries Association. Coordinator of DACST Innovation Fund project involving collaboration between Rhodes, AECI (Pty) Ptl and CSIR Bio/Chemtek groups (see below), 2000 – 2003.

Member of the Cape Biotechnology Initiative (CBI) Exco, and Coordinator of the CBI Resources Working Group (2001 – 2003);

INTERNATIONAL RECOGNITION

Research proposal reviewer for National Science Foundation, USA, (1999 and 2000)

South African National Voting Delegate for SASBMB at IUBMB International Meeting, Birmingham, UK, July 2000.

Invited session chair at international conference: Biotrans ‘99, Italy, September 1999.

Honorary Research Fellow in the Department of Biochemistry and Microbiology, University

College, London (May - Sept 2000). Member of the International Programme Committee : CHEMRAWN XIV WORLD CONFERENCE

“Toward environmentally benign processes and products” Colorado, USA, June 2001

6th Workshop of the VAAM Working group on Biotransformations, Berlin, 2001

Invited Chair: Biotrans 2003 International conference, Olomuc, Czech Republic, 2003 Invited Chair: Enzyme Engineering XVII, Santa Fe, New Mexico, 2003 Invited Chair: International Biotechnology Symposium, Santiago, Chile, 2004

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Member of the Programme Committee, World Congress on Chemical Engineering, Glasgow, 2005

Member of International Scientific Advisory Board: Advances in Engineering and Technology (AET2006) Uganda 2006, organized jointly by Makerere University, Swedish International Development Agency and Norwegian Council for Higher Education

Co-Chairman for “Extremophiles 2008”, international conference, Cape Town, 2008

Vice-President, Federation of African Societies of Biochemistry and Molecular Biology Invited Chair: IUMBM and Federation of African Societies of Biochemistry and Molecular Biology international conference, Morocco, 2009). INTERNATIONAL SCIENCE LIASON COLLABORATIONS: NRF-funded International Science Liaison projects:

Collaboration with Dr Christoph Syldatk, Karlsruhe Technical University, Germany (2003-2010) Collaboration with Dr Sergio Riva, CNR, Milan, Italy (2004-2009). Collaboration with Dr J Read and Mr C Muzariri, University of Zimbabwe (1998-2001).

Visits by international researchers to my laboratory: Professor Sergio Riva, Instituto di Biocatalisi e Riconoscimento Moleculare, Milan (2002, 2005, 2008). Professor Frances Arnold, Department of Chemical Engineering and Biochemistry, Caltech, USA (2004). Dr Daniela Monti, CNR, Milan (Sept 2005) Dr T Atkinson, TMO (Pty) Ltd, UK (2006) Professor Christoph Syldatk, Technical University, Karlsruhe (2007).

PLENARY AND INVITED LECTURES – INTERNATIONAL 1. A workshop on “Biotransformations into Practice”. Hosted by Professor H Griengl, Department

of Organic Chemistry, Technical University of Graz. Graz, Austria (May 2000). 2. IUPAC International Symposium on Green Chemistry: Lecture title: “Biotransformations and

Bioreactors”, New Delhi, India, January 2001 3. CHEMRAWN XIV World Conference “Toward environmentally benign processes and products"”

Boulder, Colorado, June 2001. 4. 6th Workshop of the VAAM-Working Group “Biotransformations”, Berlin, October 2001:

“Development of hydantoinase biocatalysts for production of amino acids”. 5. Instituto di Biocatalisi e Riconoscimento Moleculare, Milan, October 2001: “Biocatalysis for

oxidation reactions”. 6. “Bioremediation of phenolic pollutants using oxidases”. Instituto di Biocatalisi e Riconoscimento

Moleculare, Milan, October 2002 7. “Biotransformations using oxidative enzymes” Keynote lecture: Biocat 2002, International

conference on biocatalysis, Hamburg, September 2002 8. “Biotransformations with polyphenol oxidase and laccase” Department of Chemical Engineering,

Georgia State University, Atlanta, November 2003 9. Biotransformations using oxidases. Keynote lecture, Enzyme Engineering XVII, Santa Fe, 2003 10. Biotransformations with oxidases. Keynote Speaker. International biotechnology Symposium,

Chile, 2004 11. “Chemistry and biochemistry of antioxidants”. Invited speaker. Instituto di Biocatalisi e

Riconoscimento Moleculare, Milan, April 2006 12. Oxidative biotransformations and bioprocess development. Invited speaker. School of Biological

Sciences, University of Waikato, August 2006.

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13. Oxidative biotransformations and bioprocess development. Invited speaker. Department of Biological Sciences, University of Malaya, August 2006.

14. Antioxidants and their production by bioprocess. Invited speaker. Department of Biological Sciences. Science University, Malaysia, August 2006.

15. Oxidations using phenol oxidases and peroxidases. Key note speaker. American Chemical Society Annual Conference Special Symposium, Boston, August 2007.

16. A thermostable nitrilase superfamily amidase with unusual substrate selectivity. Key note address. Extremophiles, 2008, Cape Town, 2008.

PLENARY AND INVITED LECTURES - NATIONAL

1. “Biotransformations research at Rhodes University”. Biotech SA ‘97 Grahamstown. 2. Royal Society Lecture: “Biotranformations: Enzymes that work” Grahamstown, September

2000. 3. “Biodiversity: the South African position” Invited lecture at International workshop: Life in

Extreme Environments, sponsored by NASA (USA), to be held in Bloemfontein, Dec 2000. 4. Biotransformations of phenolics using microbial oxidases. Keynote lecture, SASM National

Conference, Bloemfontein, 2002. 5. Useful biotransformations” Department of Medical Biochemistry, Stellenbosch University,

September 2002 6. Biotransformations and bioremediation using oxidases. Department of Biotechnology, University

of the Western Cape, July 2003 7. “Progress in biocatalysis using oxidases” Bio ’08, Grahamstown, Jan 2008.

ACTIVITIES TOWARDS PUBLIC AWARENESS OF SCIENCE SASOL Festival of Science, Engineering and Technology, 1998.

Invited lecture: “What’s cooking?” followed by National radio interview.

SASOL Festival of Science, Engineering and Technology, 1999.

Invited lecture: “Food - What we eat and why we cook it.”

Member: National Advisory Committee for SASOL Festival of Science, Engineering and

Technology (Scifest) 1998 - 2001

SABC radio interview, August 2003

Nominated as SABC2/Checkers Shoprite Woman in Science of the Year (2005)

SOCIETY MEMBERSHIPS

South African Society for Biochemistry and Molecular Biology

European Society for Biochemistry and Molecular Biology

Water Institute of South Africa

Water Institute of South Africa - Membrane Technology Division

South African Microbiology Society

Microbiology Society (UK)

American Chemical Society

National Academy of Sciences, USA

RESEARCH RESEARCH AREAS My research is in the general areas of Biocatalysis and Technical Biology (applications of

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enzymatic processes). Specific research activities:

1) Applications of biocatalysis using oxidative enzymes and customised bioreactor systems, for production of specific compounds, particularly antioxidants

2) Investigation of the beneficial effects of natural and newly synthesised antioxidants

3) Applications of biocatalysis using oxidative enzymes and bioreactor systems for the

beneficial conversion of organic components in industrial effluents to yield valuable products.

4) Investigations of enantioselective amino acid and amide syntheses using biocatalysis

methods, specifically involving amide- and hydantoin-hydrolysing enzyme systems, and kinetic modelling of biocatalytic systems to enable prediction and optimisation of bioprocesses

5) Biotechnology approaches to accessing new and novel enzymes from biodiversity, and

bioproducts from extremophiles

6) Production of ethanol using novel biological systems and fermentations. TECHNIQUES: Chemical and biochemical analyses: NMR, IR and UV spectroscopies, mass spectrometry, GC and GC-MS, HPLC, liquid chromatography, LC-MS, protein biochemistry, microbial and biochemical screening, enzyme and bioreactor technology, ultrafiltration membrane technology. RESEARCH GRANTS Funding Body Period Project title Amount

(SAR) Water Research Commission

1993 – 1996 Membrane-based biotechnological methods for bioremediation

300 000

Water Research Commission

1997 – 2000 Biotechnological systems for conversion of industrial residues to useful chemical products

500 000

Water Research Commission

2000 – 2002 Development of bioremediation system utilising T. versicolor oxidative enzymes form phenolic bioremediation

500 000

Water Research Commission

2001 – 2002 Investigation of oxidative enzyme producing microorganisms for oxidative biotransformations

100 000

NRF 1997 – 2001 Biotransformations for South African Chemical Industry

600 000

AECI (Pty) Ltd 1993 – 1998 Development of biotransformation using oxidase enzymes

300 000

DACST Innovation Fund

2000 – 2002 New biocatalysts for the SA Chemical Industry

7 500 000

Botswana Ash 1999 – 2001 Investigation of biological-origin contaminants in soda ash

150 000

ESCOM TESP 1997 - 2001 Collaborative programme on development of membrane bioreactors

100 000

Rhodes University Joint Research

1994 - 2001 Biotransformations research 70 000

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Council NRF 2002 - 2006 Biotransformations research

ARCAM collaboration Italy-SA international liason Germany-SA collaboration

800 000 320 000 190 000 100 000

DACST LEAD 2002 - 2005 Biotransformations of ferulic acid 500 000 Billiton 2002-2004 Archaeal bioproducts 600 000 THRIP 2003 - 2004 Archaeal bioproducts 300 000 WRC 2002-2004 Bioreactor development 800 000 WRC 2004-2005 Design of wetlands 500 000 Priontex (Ltd) 2005 Development of disinfectant dressings 67 000 THRIP 2005 Development of disinfectant dressings 67 000 WRC 2005 Beneficiation of citrus processing

wastewater 145 000

WRC 2005 Production of hydroxytyrosol from olive wastewater

197 000

NRF 2006 Search for industrial oxidases 315000 WRC 2005-2006 Citrus waste beneficiation 168000 WRC 2005-2006 Olive waste beneficiation 197000 TMO (Pty) Ltd 2005-2006 Commercial ethanol production 128000 FMC Inc. 2006 Biotrans production of catechols 360000 Winetek 2006 Aroma compounds in wine 235000 THRIP 2006 Aroma compounds in wine 235000 NRF 2007 Search for industrial oxidases 330 000 NRF 2007 Effects of antioxidants 150 000 NRF 2007 Efficient bioprocesses 430 000 WRC 2007 Wastewater beneficiation 320 000 WRC 2007 Wetland development 220 000 TMO (Pty) Ltd 2007 Commercial ethanol production 280 000 FMC Inc. 2007 Biotrans production of catechols 90 000 Winetek 2007 Aroma compounds in wine 240 000 THRIP 2007 Aroma compounds in wine 190 000 NRF 2008 Search for industrial oxidases 250 000 NRF 2008 Effects of antioxidants 125 000 NRF 2008 Efficient bioprocesses 306 000 WRC 2008 Wastewater beneficiation 290 000 WRC 2008 Wetland development 420 000 WRC 2008 Energy from Wastewater 400 000 TMO (Pty) Ltd 2008 Commercial ethanol production 384 000 Winetek 2008 Aroma compounds in wine 234 000 THRIP 2008 Aroma compounds in wine 80 000 NRF 2009 Search for industrial oxidases 310 000 NRF 2009 Effects of antioxidants 160 000 NRF 2009 Efficient bioprocesses 380 000 WRC 2009 Wastewater beneficiation 310 000 WRC 2009 Wetland development 400 000 TMO (Pty) Ltd 2009 Commercial ethanol production 390 000 CPUT 2009 Research grant 353 000

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COLLABORATORS Professor D A Cowan, Department of Biotechnology, University of the Western Cape, (Current collaboration on Biodiversity Exploitation, molecular technologies and biocatalysis, and the search for novel enzymes). Professor Sergio Riva, Instituto di Biocatalisi e Riconoscimento Moleculare, Milan (Current project on laccase biotransformations, funded by SA-Italy agreement , 2002 - present). Professor Christoph Syldatk, Technical University of Karlsruhe (Current project on biodiversity and application of amide cleaving enzymes, funded by Germany-SA agreement). Professors Pierre van Rensburg and Florian Bauer, Institute of Wine Biotechnology, University of Stellenbosch (recent collaboration on aroma compounds in wine). Professor S Kidson and Dr L Davids, Department of Human Biology, UCT Medical School (Current collaboration on cellular effects of antioxidants). Dr K Clarke, Department of Process Engineering, University of Stellenbosch (Current project on bioreactor processes). Prof S T L Harrison, Department of Chemical Engineering, UCT (Current collaboration on bioreactor processes). Professor A Bommarius, Georgia State University (Previous collaboration on NADPH oxidase). Dr R A Dorrington, Department of Biochemistry and Microbiology, Rhodes University (Current collaboration on biotransformations using hydantoinases and related enzymes). Professor P Rose, Department of Biochemistry and Microbiology, Rhodes University (Recent collaboration on bioremediation of industrial residues). Professor R Farrell, Department of Microbiology, University of Waikato (Current collaboration on oxidase enzyme applications)

STUDENT SUPERVISION Honours students graduated: 1991 – 2002: 23 students STUDENTS SUPERVISED PREVIOUSLY Masters theses awarded: 2000: Mr V Phehane (Co-supervisor: Dr R Dorrington) Black, male 2001: Ms P Nganwa Black, female 2000: Ms I Russell White, female 2001: Ms M Joseph Indian, female

2002: Ms T Notshe Black, female 2002: Ms C Mhlanga Black, female 2004: Ms B Ting Indian, female 2005: Mr S Makhongela Black male 2005: Mr C Werner White male 2006: Mr J Germanis White male 2008: Mr F Olaofe Black male

PhD theses awarded 1999: Mr W Leukes, Biotechnology (Co-supervisor) Coloured, male 1999: Mr W Edwards (Co-supervisor) White, male 2002: Ms A Boshoff White, female 2003: Mr S Kirchmann White, male 2003: Ms I Foster White, female

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2004: Mr D Ryan White, male 2005: Mr B Bulawayo Black, male 2006: Mr C Garcin White male 2007: Ms N Chigorimbo-Tsikiwa Black, female 2007: Mr S Ncanana Black, male 2007: Mr R Duerr (Co-supervisor) White, male 2009: Ms C Vengadajellum Indian, female 2009: Mr I Chiyanzu Black,male

PRESENT POSTGRADUATE STUDENTS (2007):

PhD: Ms C Goodwin: Biocatalysis using novel oxidases White, female Ms E Adelakun: Benefits of antioxidants Black, female

MSc: Mr C Pries. Antioxidant compounds from grapes White, male

Ms M Ngobeni: Novel hydantoinase reactions Black, female Ms R Zwane: Antioxidants effects in cells Black, female Ms K Maele: Search for new industrial oxidases Black, female Ms N Davids: Development of novel oxidase biocatalysts Colored, female Ms C Mupure: Wastewater beneficiation Black, female Ms A Gabathuse: Developing amidase biocatalysis Black, female

Foreign students: Mr R Duerr, PhD, University of Stuttgart 2003- 2007; (Co-supervised with Professor C Syldatk) Ms A Schueller, Masters, Technical University of Karlsruhe (2008) Postdoctoral fellows: Mr C Garcin. Development of bioreactors form bioremediation processes (2006-2007). Dr A Glowacka: Biocatalytic production of amides (2006-1007)

Dr J Zvimba: Oxidative biotransformations (2006) Dr S Emenour: Oxidase characterisation (2007-2008).

Dr M le Roes: Selection of industrially relevant oxidases (2007 - present) Dr N Khan: Applications of new peroxidises (2009 – present) Dr A Parker: Benefits of antioxidants (2009 – present) Dr C Bressa: Bocatalysis using thermostable nutrile hydratase (2009 – present) AWARDS TO STUDENTS UNDER SUPERVISION C Garcin: National Innovation Competition: Provincial prize S Ncanana: SAIChE Chemical technology Prize, 2nd place, 2004 C Sheridan: SAIChE Chemical Technology Prize, 1st place, 2005

PUBLICATIONS REFEREED INTERNATIONAL JOURNALS

1. S G Burton and P T Kaye (1989), A convenient preparation of carboxylic acid anhydrides using a

"supported" phosphorus pentoxide reagent, Synthetic Communications, 19 (19), 3331.

2. S G Burton and P T Kaye (1992), Isolation and characterisation of sulphur compounds from Tulbaghia violacea, Planta Medica, 58, 295.

3. S G Burton, J R Duncan, P T Kaye and P D Rose (1994), Activity of mushroom polyphenol

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oxidase in organic medium, Biotechnology and Bioengineering, 42(8), 938.

4. S G Burton (1994), Biocatalysis with polyphenol oxidase: a review, Catalysis Today, 22, 459-487.

5. S G Burton and J R Duncan (1995), Analysis of enzyme kinetic measurements for an organic-

medium biocatalyst, Biotechnology Techniques, 9(1), 13-18.

6. S G Burton and J R Duncan (1995), Activation of mushroom polyphenol oxidase in organic media by the detergent SDS, Biotechnology Letters, 17(6), 627-630.

7. Burton S G and Kirchmann S. (1997) Optimised detergent-based extraction of a chloroplast

membrane-bound enzyme: Polyphenol oxidase from tea (Camellia sinensis). Biotechnol. Techniques, 11 (9), 645- 648.

8. Burton S G, Boshoff A, Edwards W and Rose P D. (1998). Biotransformation of phenols using

immobilised Polyphenol oxidase. J. Molec. Catal. (Enzymic, 224), 411 – 416.

9. Burton S G, Dorrington R A, Hartley C, Kirchmann S, Buchanan K, and Phehane V. (1998). Production of enantiomerically pure amino acids: characterisation of South African hydantoinases and hydantoinase-producing bacteria. J. Molec. Catal. (Enzymic, 224), 301 – 305.

10. Ryan D R, Russell,A K, Leukes W D, Rose P D and Burton, S G. (1998) Suitability of a modified

capillary membrane for growth of fungal biofilms. Desalination: Special Issue on Membrane Technology 115(3), 303 – 306.

11. Boshoff A, Edwards W, Leukes W D, Rose P D and Burton S G. (1998) Immobilisation of

polyphenol oxidase on nylon and polyethersulphone membranes: effect on product formation. Desalination: Special Issue on Membrane Technology 115(3), 307 – 312.

12. Hartley C, Kirchmann S, Burton S G and Dorrington R A (1998). Evidence for diversity among

Agrobacterium strains which hydrolyse D,L-5-substituted hydantoins. Biotech Letters 20, 707 - 710.

13. Edwards W, Bownes R, Leukes W D, Jacobs E, Sanderson R, Burton S G, and Rose P D. (1999)

A capillary membrane bioreactor using immobilised polyphenol oxidase for the removal of phenols from industrial effluent. (Enzyme and Microbial Technology, 24 (3-4), 209 - 217.

14. Russell I and Burton S G (1999). An immobilised-enzyme bioprobe using polyphenol oxidase to

detect low concentrations of phenols. Analytica Chimica Acta (389, 1-3), 161 - 170.

15. Edwards W, Leukes W D, Rose P D, Burton S G. (1999) Immobilisation of tyrosinase on chitosan coated polysulphone capillary membranes for phenolic effluent bioremediation. Enzyme and Microbial Technology, 25, 769 - 773.

16. Burton S G, Kaye P T and Wellington K. (2000). Designer Ligands. Part 5. Synthesis of

polydentate biphenyl ligands. Synthetic Communications 30(3), 511-522.

17. Buchanan K, Burton S G, Dorrington R A, Matcher G, Skepu Z. (2001) A novel Pseudomonas putida strain with high levels of hydantoin-converting activity, producing L-amino acids. J. Molec. Catal. B (Enzymic) 11, 397-406

18. Hartley C J, Manford F, Burton S G and Dorrington R A. (2002) Overproduction of hydantoinase

and N-carbamolyamino acid amidohydrolase enzymes by regulatory mutants of Agrobacterium tumefaciens. Applied Microbiology and Biotechnology 57, 43-49.

19. Luke, A K and Burton, S G. (2001) A novel application for Neurospora crassa: Bioremediation of

phenols in a membrane bioreactor. Enzyme and Microbial Technology 21, 348-352

20. Burton, S G. (2001) Development of Bioreactors for Application of Biocatalysts in

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Biotransformations and Bioremediation. (Invited paper). Pure and Applied Chemistry 73, 77-83.

21. Burton S G, Cowan D A and Woodley J M. (2002) The Search for the Ideal Biocatalyst.

(Invited review) Nature Biotechnology, 20, 37-45.

22. Burton, S G and Cowan, D A. (2002) Biotechnology Issues for Developing Countries. (Invited review). Electronic Journal of Biotechnology 5, 03. Http://ejb.ucv.cl/content/vol5/issue1/issues/03/index.html

23. 1Ingrid M. Foster, 1Rosemary D. Dorrington and 2Stephanie G. Burton (2003). Enhanced

hydantoinase and N-carbamoylase activity on immobilisation of Agrobacterium tumefaciens strain RU-ORPN1. Biotech Letters, 25, 67-72.

24. Boshoff, A, Burton M H, Burton S G. (2003) Optimisation of catechol production using

membrane-immobilised polyphenol oxidase: a modeling approach. Biotech. Bioeng. 83, 1-7.

25. Burton S G. (2003) Oxidative biotransformations using microbial oxidases. Invited review, TIBTECH (Trends in Biotechnology), 21, 543-549

26. Burton, S G. (2003) Biotransformations with phenol oxidases and laccase: a review. Current Organic Chemistry 7, 1317-1331

27. Cowan D A, Arslanoglu A, Burton S G, Baker G C, Cameron R A, Smith J J and Meyer Q. (2004)

Metagenomics, Gene Discovery and the Ideal Biocatalyst. Biochem Soc. Trans. Special Edition Biochem. Soc. Trans. 32:298-302

28. Matcher GF, Burton SG, Dorrington RA. (2004) Mutational analysis of the hydantoin hydrolysis

pathway in Pseudomonas putida RU-KM3s. Applied Microbiology and Biotechnology 65: 391-400.

29. Burton, S G and Dorrington R A. 2004. Hydantoin-hydrolysing enzymes for enantioselective

production of amino acids: new insights and applications. (Invited paper) Tetrahedron Asymmetry. 15, 2737-2741.

30. Sheridan C M, Bauer, F F, Burton S G, and Lorenzen, L. A (2005) Critical Process Analysis of

Wine Production to Improve Cost, Quality and Environmental Performance. Journal of Water Science and Technology 51, 39-46.

31. Ryan R, Leukes W and Burton S. (2005) Fungal bioremediation of phenolic wastewaters in an

airlift reactor. Biotechnology Progress. 21(4), pp 1068-1074. 32. Craig Sheridan, Jochen Petersen and Stephanie Burton, Johann Rohwer (2005). Constructed

wetlands for winery effluent remediation . Chemical Technology, 4. 33. Stafford, WHL, Baker, GC, Brown, SA, Burton SG and Cowan DA. (2005)

Bacterial diversity in the rhizosphere of Proteaceae species. Environ. Microbiol. 7:1755-1768. 35. R. Dürr, O. Vielhauer, S.G. Burton, D.A. Cowan, A. Puñal1, P.F.B. Brandão1, A.T. Bull, C. Syldatk

Distribution of hydantoinase activity in bacterial isolates from geographically distinct environmental sources. (2006) J Molecular Catal B: enzymic 39: 160- 165.

36. Daniel Ryan, Winton Leukes and Stephanie Burton. Improving the bioremediation of phenolic

wastewaters by Trametes versicolor (2007) Bioresource Technology 98: 579-587

37. Bulawayo B, Dorrington RA and Burton SG. (2007) . Enhanced operational parameters for amino acid production using hydantoin-hydrolysing enzymes of Pseudomonas putida strain RUKM3s immobilised in Eupergit® C. Bulawayo B, Dorrington RA and Burton SG. Enzyme Microbial Technology (40) 533-539

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38. Sandile Ncanana and Stephanie Burton Oxidation of 8-hydroxyquinoline catalyzed by laccase from Trametes pubescens yields an antioxidant aromatic polymer. (2007) J Molec. Catal. B Enzymatic. 44, 66-71.

39. Sandile Ncanana and Stephanie Burton. Enzymatic synthesis of poly (8 hydroxyquinoline) by

Trametes pubescens laccase. (2006) Proceedings, SAICHE Annual Conference. 40. Jason P. Germanis and Stephanie G. Burton. An investigation into the conditions of polyphenol

extraction from red grape marc (Vitis vinifera). (2006) Proceedings, SAICHE Annual conference.

41. Q.C. Meyer, S.G. Burton and D.A. Cowan. Subtractive hybridization magnetic bead capture: A

new technique for the recovery of full length ORFs from the metagenome. (2007) Biotechnol J. 2, 36-40.

42. Q.C. Meyer, S.G. Burton and D.A. Cowan. Detection of bacterial laccase genes in the

metagenome and the recovery of full length ORFs by subtractive hybridization magnetic bead capture. (2007) [ BMC Microbiology, accepted under revision]

43. Konanani J. Rashamuse, Stephanie G. Burton, William Stafford and Don. A. Cowan Molecular

characterization of a novel family VIII esterase from Burkholdaria multivorans UWC10. (2007). J Mol Microbiol Biotechnol 13:181–188

44. Makhongela H. S., Agarkar V. B., Sewell, B.T., Cameron R. A, Cowan D. A., and Burton S. G.

Molecular characterization and immobilization of D-specific amidase from Geobacillus pallidus. (2007) Applied Microbiol. Biotechnol, 75, 801-811

45. M Maria Estela Silva-Stenico, Caryn J. Vengadajellum, Hussnain A. Janjua, Stephanie G. Burton,

Sue T. L. Harrison, Don A. Cowan Degradation of low-rank coal by Trichoderma atroviride ES 11. (2007) J. Industrial Microbiol. and Biotech 43: 625-631

46. Sandile Ncanana, Lara Baratto, Lucia Roncaglia, Sergio Riva and Stephanie G Burton. Laccase-

mediated oxidation of totarol. Advanced Synthesis and Catalysis 349, 1507-1513.

47. Shaun Kirchmann, Petrus van Zyl, Dean Brady, Nasreen Abrahams, Sonia Rech, Rosemary Dorrington, Stephanie Burton. (2007). A dual phase fermentation protocol for the production of hydantoinase and carbamoylase by the wild type Pseudomonas putida RU-KM3. Enzyme Microbial Technology 41(4) 539-545

48. K.J. Rashamuse, S.G. Burton and D.A. Cowan. (2007) A novel recombinant ethyl ferulate

esterase from Burkholderia multivorans. Journal of Applied Microbiology 103 (5), 1610-1620

49. R. Dürr, A. Neumann, O. Vielhauer, J. Altenbuchner, S.G. Burton, D.A. Cowan, C. Syldatk.

(2008) Genes responsible for hydantoin degradation of a halophilic Ochrobactrum sp. G21 and Delftia sp. I24 – new insight into relation of D-hydantoinases and dihydropyrimidinases. J. Molec. Catal. Enzymatic 52-53, 2-12.

50. B.O. Oboirien, S.G. Burton, D. Cowan, S.T.L. Harrison. (2008). The effect of the particulate

phase on coal biosolubilisation mediated by Trichoderma atroviride in a slurry bioreactor. Fuel Processing Technology, 98, 123-130.

51. Nyaradzo T. L. Chigorimbo-Murefu, Sergio Riva, Stephanie G. Burton (2008). Lipase-catalysed

synthesis of esters of ferulic acid with natural compounds to give improved antioxidant properties. J. Molec. Catal. Enzymatic (in press).

52. Marilize le Roes-Hill, Jeffrey Rohland, Paul R. Meyers, Don A. Cowan and Stephanie G. Burton

Streptomyces hypolithicus sp. nov., a novel streptomycete isolated from a hypolith community, Antarctica. Internationat. J. Soc. Gen. Microbiol. (in press)

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53. Babalola, OO, Kirby, BM, Le Roes-Hill, M, Cook, AE, Cary, SC, Burton, SG, Cowan, DA. Phylogenetic analysis of actinobacterial populations associated with Antarctic Dry Valley mineral soils. Envir. Microbiol. In Press.

54. Taylor, M, Tuffin, M, Burton, SG, Eley, K, Cowan, DA. (2008). Microbial responses to solvent

and alcohol stress. Biotechnol. J. 2008, 3, 1388–1397

55. Enhanced hydantoin-hydrolyzing enzyme activity in an Agrobacterium tumefaciens strain with two distinct N-carbamoylases. Meesbah Jiwaji, Carol J. Hartley, Sally-Ann Clark, Stephanie G. Burton, and Rosemary A. Dorrington. (2009) Enzym. Microb. Technol. 44, 203–209

56. Taylor, M, Eley, K, Martin, S, Tuffin, IM, Burton, SG, Cowan DA (2009) Thermophilic

ethanologenesis. Future prospects for second generation ethanol production. Trends Biotechnol. In Press

57. Marilize Le Roes-Hill, Candice Goodwin and Stephanie Burton. (2009). Phenoxazinone synthase:

what’s in a name? Trends in Biotechnology. Available online, March 2009

58. Performance of an amidase-immobilised membrane bioreactor. R du Preez, KG Clarke, D Cowan and SG Burton. Proceedings, WCCE8, July 2009.

PAPERS IN PREPARATION AND SUBMITTED 1. Rohr, LM, Mashaphu, N, Sheridan, C, Burton, SG, and Cowan, DA. Seasonal shifts of the

microbial community structure in a winery waste-impacted wetland soil. ISME J. Submitted. 2. Development of a membrane immobilised amidase bioreactor system. R. Du Preez, K.G. Clarke*,

H. Velankar, D. Cowan and S.G. Burton. CATSA August 2008. ,

3. Immobilization of a nitrile hydratase (NHase) from Geobacillus pallidus RAPc8: a way to overcome substrate inhibition and enhance stability. Idan Chiyanzu, Don A. Cowan and Stephanie G. Burton. Submitted, Jun 2009.

4. Olaofe, OA, Burton, SG, Cowan, DA, Harrison, STL. Optimisation of production of a thermostable

amidase through optimising biomass concentration and IPTG induction in a recombinant Escherichia coli. J. Biotechnol. Submitted.

5. Modelling of immobilised enzyme membrane bioreactor performance. R du Preez, KG Clarke and

SG Burton, submitted, June 2009. BOOK CHAPTERS 1. Biocatalysis – a complementary approach in green chemistry Stephanie G Burton In Inter-University Consortium “Green Chemistry in Africa,” Green Chemistry Series, Editor: P Tundo, Italy. IUPAC-INCA (2002).

2. Archaea in Biotechnology Cowan, DA and Burton, SB (2002). Encyclopaedia of Environmental Microbiology. Wiley, Publ., pp 259-276.

3. Oxidising enzymes in multi-step biotransformation processes. Stephanie G Burton and Marilize le Roes-Hill. Invited chapter, Multi-step enzyme catalysis, Editor: Eduardo Garcia-Junceda, Wiley, 2008.

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INVITED ARTICLES 1. S G Burton. (1997). Biotransformations with membrane bioreactor technology. WISA-MTD

Newsletter 32, 6. 2. S G Burton. (1997) Transforming phenols using polyphenol oxidase. Chemical Process Alert

3, (41), 3. 3. S G Burton. (1997) Transform phenols with polyphenol oxidase. Industrial Bioprocessing 19

(11), 2. 4. R A Dorrington and S G Burton (2000) Microbes for the New millenium – biocatlayts, hydantoins

and the fine chemical industry. Forum Microbiologium (Jan 2000). 5. D A Cowan and S G Burton . (2000) Microbial Biodiversity: Exploration, Conservation and

Exploitation. Forum Microbiologicum, Spring/summer 2000, pp. 10-13. 6. Stephanie G Burton and Don A Cowan. The exploitation of South African Microbial Diversity.

SASBMB Newsletter 4(2) Dec 2000, pp. 10-11 PATENTS

1. Method for continuous production of secondary metabolites using hollow fibre membranes. WRC

patent. W D Leukes, S G Burton, P D Rose, E Jacobs and R D Sanderson ( 95/7366, International patent accepted 1996). Patent held in UK, USA and Europe.

2. Immobilised enzyme bioprobe. WRC patent. S G Burton and I M Russell. (97/0227, accepted). Patent held in SA.

3. Novel organisms, their use and the production of D-amino acids. R A Dorrington, S G Burton and C

Hartley. Patent ZA99/5981 (Accepted international, 2002). 4. Production of antioxidants from olive waste. S Burton and C Garcin. (PCT Registered 2006) 5. Bioconversion of asphaltene by Trametes sp. C Vengadajellum and S G Burton (SA Provision

registered November 2008) TECHNICAL REPORTS 1. S G Burton, E P Jacobs, P D Rose and R D Sanderson (1995). Research into the use of capillary

membranes for the biotechnological treatment of industrial effluents. Water Research Commission, South Africa.

2. S G Burton, A Boshoff, W Edwards, E P Jacobs, W D Leukes, P D Rose, A K Russell, I M Russell, D

Ryan (1998). Membrane-based biotechnological systems for treatment of organic pollutants. Water Research Commission, South Africa.

3. S G Burton, A Boshoff, I Foster, K Koteshwar, A Luke, C Mhlanga, P Nganwa, T Notshe, and D

Ryan. Bioreactor systems for the conversion of organic compounds in industrial effluents to useful products (2001). Water Research Commission, South Africa.

4. Hydantoinase Project Technical progress reports submitted to AECI (Pty) Ltd.1997, 1998, 1999

and Innovation Fund, 2000 – 2004.

5. S G Burton, D R Ryan, L van Wyk (2003). Bioreactor systems using the white-rot fungus Trametes for bioremediation of industrial wastewater. Water Research Commission, South Africa.

6. S G Burton, D Cowan, C Garcin, A van Schalkwyk and C Werner. (2005). A customised

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bioreactor for beneficiation and bioremediation of effluents containing high value organic chemicals. Water Research Commission, South Africa

7. S G Burton, C Garcin, J H Aucamp.(2006). Beneficiation of wastewaters from the South African

citrus industry - a feasibility study. Water Research Commission, South Africa. 8. S Burton, C Sheridan, J Law-Brown, M Le Roes, D Cowan, L Rohr and N Mashaphu. (2006).

Integrated research for use in constructed wetlands for treatment of winery wastewater. Water Research Commission, South Africa.

9. C. J. Garcin and S. G. Burton. (2006). Implementation of a generic membrane-based system for

beneficiation and treatment of agro-industrial wastewater. Water Research Commission, South Africa.

10. S. Burton, B. Cohen, S. Harrison, S. Pather-Elias, W. Stafford, R. van Hille, and H. von

Blottnitz. (2008) Energy from wastewater – a feasibility study. Water Research Commission, South Africa

CONFERENCE PRESENTATIONS INTERNATIONAL CONFERENCES

1. S G Burton, J R Duncan and P T Kaye, Investigation of conditions for application of polyphenol oxidase in organic media. IUPAC-NOST International Symposium on Enzymes in Organic Synthesis, New Delhi, 1992.

2. S G Burton, J R Duncan, P T Kaye, Mushroom polyphenol oxidase: Biocatalysis and

biomimetics. European Symposium on Biocatalysis, Graz, 1994.

3. W Leukes, S Burton, E Jacobs and P Rose, Monitoring of a hollow fibre membrane-immobilised fungal enzyme production system. First International Workshop on Catalytic Membranes, Lyon, France, September 1994.

4. W Edwards, W. Leukes, R. Lalloo, S. Burton,. E. Jacobs, R. Sanderson and P. Rose. Removal of

phenols from wasewater using soluble and immobilised fungal polyphenol oxidase. First International Workshop on Catalytic Membranes, Lyon, France, September 1994.

5. Workshop on Biosensors and Biological Techniques in Environmental Analysis, Paris,

September 1994.

6. S G Burton and S Kirchmann. Isolation and purification of tea polyphenol oxidase. Tea Research Council Foundation of Central Africa, First Regional Tea Research seminar, Blantyre, Malawi, 1995.

7. S G Burton, R A Dorrington, M N Gardner, C J Hartley, and S Kirchmann. Hydantoin-

hydrolysing activity of microbial enzymes. Biotrans ‘95, Warwick, UK, 1995.

8. S G Burton, W Edwards, W D Leukes, P D Rose, E P Jacobs and R D Sanderson. The effects of immobilisation of polyphenol oxidase in a hollow-fibre membrane bioreactor on its monophenolase activity. Biotrans ‘95, Warwick, UK, 1995.

9. W Leukes, S G Burton, W Edwards, E Jacobs, R Sanderson and P D Rose. A critical

investigation of the rate of cresol removal by Phanerochaete chrysosporium in a membrane reactor. SGM/AMS Meeting on Bioremediation, Aberdeen, 1995.

10. W Edwards, W D Leukes, S G Burton, E P Jacobs, R D Sanderson and P D Rose.

Dephenolisation of a synthetic industrial wastewater using hollow-fibre immobilised polyphenol oxidase. SGM/AMS Meeting on Bioremediation, Aberdeen, 1995.

11. S G Burton, R A Dorrington, M Gardner, C Hartley and S Kirchmann. Isolation and

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characterisation of South African hydantoinase-producing bacterial strains. SGM/AMS Meeting on Bioremediation, Aberdeen, 1995.

12. S G Burton, W Edwards, W D Leukes, P D Rose, E P Jacobs and R D Sanderson. The effects of

immobilisation of the activity of polyphenol oxidase in a hollow-fibre membrane bioreactor. Enzyme Engineering XIII, Meeting of the Engineering Foundation, San Diego, 1995.

13. Edwards W, Boshoff A, Leukes W, Jacobs E, Sanderson R, Saayman H, Burton S and Rose P.

Continuous Treatment of phenol-polluted waters in a novel capillary membrane bioreactor. ICOM ‘96, Japan, 1996.

14. Leukes W, Jacobs E, Sanderson R, Saayman H, Burton S and Rose P. Continuous production of

lignin peroxidase by Phanerochaete chrysosporium in a novel capillary membrane bioreactor for the degradation of aromatic pollutants. ICOM ‘96, Japan, 1996.

15. Burton S G, Rose P D, Boshoff A, and Edwards W. Biotransformation of phenols using

immobilised polyphenol oxidase. Gordon Research Conference on Environmentally Benign Organic synthesis, Oxford, 1997.

16. Burton S G, Dorrington R A, Hartley C, Kirchmann S, Buchanan K and Phehane V. Production

of enantiomerically pure amino acids: Characterisation of South African hydantoinases and hydantoinase-producing bacteria. Gordon Research Conference on Environmentally Benign Organic synthesis, Oxford, 1997.

17. Leukes W D, Jacobs E P, Sanderson R D, Burton S G and Rose P D. Fungal dynamics in a

membrane gradostat reactor degrading aromatic pollutants. ECB8 Symposium on Biological Treatment of Organic Pollutants. Hungary, 1997.

18. Edwards W, Leukes W, Jacobs E P, Sanderson R, Burton S G and Rose P D. Immobilised

tyrosinase on chitosan-coated membraens for phenolic industrial affluent bioremediation. National American Membrane Society, USA, 1997.

19. Burton S G, Dorrington R A, Hartley C, Kirchmann S, Buchanan K and Phehane V. Production

of enantiomerically pure amino acids: Characterisation of South African hydantoinases and hydantoinase-producing bacteria. Biotrans ‘97, Montpellier, France, 1997.

20. Burton S G, Rose P D, Boshoff A, and Edwards W. Biotransformation of phenols and the

influence of method of immobilisation on polyphenol oxidase activity. Biotrans ‘97, Montpellier, France, 1997.

21. Burton S G, Dorrington R A, Hartley C, Kirchmann S, Matcher G and Phehane V.

Characterisation and application of hydantoinases and hydantoinase-producing bacteria. Oral. 20th Symposium on Biotechnology for Fuels and Chemicals, Gatlinburg, Tenn., USA, 1998.

22. S G Burton, A Boshoff, W Edwards, W Leukes, D Ryan, T Russell, I Russell. Bioremediation

and Biotransformation of organic pollutants using oxidative enzymes. 20th Symposium on Biotechnology for Fuels and Chemicals, Gatlinburg, Tenn., USA, 1998.

23. S G Burton, K Buchanan, G Matcher and Z Skepu. A Novel Pseudomonas strain with high

levels of hydantoinase activity. Biotrans ’99, Sicily, 1999.

24. C J Hartley, S G Burton, R A Dorrington. Bioctalytic prpoduction of D-amino acids – Agrobacterium tumefaciens RU-OR mutants which overproduce hydantoinase and N-carbamylamino acid aminohydrolase. Biotrans ’99, Sicily, 1999.

25. R A Dorrington, C J Hartley, S G Burton. Regulatory and molecular characterisation of

hydantoin hydrolysing enzyme systems in Agrobacterium tumefaciens RU-OR. Biotrans ’99, Sicily, 1999.

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26. S G Burton, A Boshoff, W Edwards, K Koteshwar, P Nganwa, D Ryan and T Luke Biotransformation of aromatics using oxidative enzymes. Enzyme Engineering, XIV, USA, 1999.

27. S G Burton, M H Burton and A Boshoff. Optimisation of catechol production using immobilised

polyphenol oxidase. Enzyme Engineering, XIV, USA, 1999.

28. S G Burton. Biotransformations into Practice. Keynote. Workshop hosted by Professor H Griengl, Department of Organic Chemistry, Technical University of Graz, Austria 2000.

29. Matcher, G, Dorrington R and Burton, S. Novel hydantoinase and NCAAH activity in

Pseudomonas putida strain RU-KM3s. ASM conference on Biocatalaysis, Biotransformations and Bioremediation, Puerto Rico, 2001.

30. Burton S, Boshoff, A, Ryan, D, Luke, T, Mhlanga C, Koteshwar K, Notshe T. Biotransformation

of phenolics using oxidative enzymes VI, Potsdam, 2001.

31. Burton S, Foster I, Kirchmann S, Bulawayo B and Dorrington R. Development of hydantoinase biocatalysts for production of D-amino acids. ASM conference on Biocatalaysis, Biotransformations and Bioremediation, Puerto Rico, 2001.

32. Ryan D R, Leukes W L and Burton S G. The Development of a Bioreactor System Using

Trametes versicolor for the Bioremediation of Industrial Wastewater 8th International Conference on Biotechnology in the Pulp and Paper Industry in Helsinki, Finland 2001.

33. S Burton, Biotransformations using microbial oxidases. Keynote. Biocat 2002, Hamburg.

34. Don Cowan1, Stephanie Burton2, Sara Sjoling3, Craig Cary4, Samantha Whiting5 and Nicholas

Russell6. The Microbiology of Antarctic Deserts Extremophiles, 2002, Naples October 2002.

35. Cowan D A, Arslanoglu A, Burton S G, Baker G C, Cameron R A, Smith J J and Meyer Q. Metagenomics, Gene Discovery and the Ideal Biocatalyst. Thermophiles 2003, Exeter, UK.

36. Cowan D, Burton S, Sjoling S, Cary C, Whiting S and Russell N. The Microbiology of Antarctic

Deserts. Biotrans 2003, Olomuc, , 2003

37. S Burton and D Ryan. An airlift bioreactor for laccase production by Trametes, and conversion of phenolics . Keynote. Enzyme Engineering XVII, Santa Fe, 2003

38. S Burton, R Dorrington and B Bulawayo. Amino acid production by hydantoinases of

Pseudomonas putida RUKM3s and covalent immobilisation for enhanced biocatalytic activity. Enzyme Engineering XVII, Santa Fe, 2003

39. Sheridan, C, Bauer, F, Burton S, Lorenzen L. A critical process analysis of wine production to

improve cost, quality and environmental performance. 3rd International Specialised Conference on sustainable Viticulture and Winery Wastes Management. Barcelona, 2004.

40. Burton S. Biocatalysis using oxidases. Keynote. International Biotechnology Symposium,

Santiago, Chile, 2004

41. Chigorimbo-Murefu, N.T.L., Cowan D A, Mitra R. and Burton S.G. Biocatalytic transformations of ferulic acid derived from maize waste. 12th International Biotechnology Symposium and Exhibition, Santiago, 2004.

42. C.J. Vengadajellum, M.E. Stenico; S.T.L. Harrison; D.A. Cowan, S.G. Burton. Bioreactor

systems for the biosolubilisation of low rank coal. International Biotechnology Symposium, October 2004, Santiago, Chile

43. Stephanie G Burton Bernard T Bulawayo, Ingrid M Foster, Rens van den Boomen and

Rosemary D. Dorrington. A novel approach to production of amino acids from hydantoins using combined biocatalysts International Congress in Chemical Engineering, Scotland, 2005

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44. Stephanie G Burton Bernard T Bulawayo, Ingrid M Foster, Rens van den Boomen and

Rosemary D. Dorrington.. Development of a novel bioprocess for production of amino acids from hydantoin substrates using two biocatalysts. Biotrans 2005, Delft, 2005.

45. Stephanie G Burton, Sandile Ncanana, Blanche Ting and Nyaradzo Chigorimbo-Murefe.

Sequential biocatalytic oxidation steps to produce novel antioxidant compounds. Multistep enzyme catalysed bioprocesses 2006, Graz, 2006.

46. Stephanie G Burton, H S Makhongela, , A E Glowacka, T N Sewell, D A Cowan. Applications of

a thermostable nitrilase superfamily amidase with unusual substrate selectivity. Biotrans 07, Oviedo, Spain.

47. Stephanie G Burton. Biocatalysis using phenol oxidases and peroxidases. Keynote. American

Chemical Society Annual Meeting Special symposium. Boston USA., August 2007.

48. Exploiting actinomycete biodiversity in the search for an ideal oxidase biocatalyst. M Le Roes-Hill, K Maele , A Bull and S Burton. 14th International Symposium on the Biology of Actinomycetes. Newcaste, UK. August 2007.

49. Recovery of antioxidants from agro-industrial wastewaters, S.G. Burton and C.H. Mupure,

WISA 08, Sun City, May 2008

50. Kerry Horne, Andrew Nel, Don Cowan, Kirstin Eley and Stephanie Burton, Fermentation of lignocellulosic waste by a novel Bacillus licheniformis isolate, Extremophiles 08, Sept 2008

51. Davids, N, le Roes Hill, M, Burton, SG.(2008) Deep-sea actinomycetes strains having the ability

to degrade Crystal Violet.A potential source of novel oxidative enzymes for industrial application. Extremophiles 2008. Cape Town, South Africa

SOUTH AFRICAN CONFERENCES

1. S G Burton, J R Duncan, P T Kaye and P D Rose, Biocatalysis in organic medium: the polyphenol oxidase system. 10th Annual Congress of the South African Biochemical Society, Pietermaritzburg, 1991.

2. S G Burton, J R Duncan and P T Kaye, Conditions for polyphenol oxidase activity in organic medium. 11th Annual Congress of the South African Biochemical Society, Sun City, 1992.

3. S G Burton, J R Duncan, P T Kaye, and P D Rose, Activity of mushroom polyphenol oxidase in organic medium. Biotech SA '93, Grahamstown, 1993.

4. S G Burton, J R Duncan and P T Kaye, Biocatalysis and biomimetics with mushroom polyphenol oxidase. 12th South African Biochemistry Society Congress, Stellenbosch, 1994.

5. W Leukes, S Burton, E Jacobs and P Rose, Monitoring of a hollow fibre membrane-immobilised fungal enzyme production system. 8th Biennial Congress of the South African Society for Microbiology, Grahamstown, 1994.

6. W Edwards, W Leukes, R Lalloo, S Burton, E Jacobs and P Rose, Removal of phenols from wastewater using soluble and immobilised fungal polyphenol oxidase. 8th Biennial Congress of the South African Society for Microbiology, Grahamstown, 1994.

7. S G Burton, J R Duncan and P T Kaye, Biocatalysis and biomimetics with mushroom polyphenol oxidase. 12th South African Biochemistry Society Congress, Stellenbosch, 1994.

8. W Edwards, W Leukes, R Lalloo, S Burton, E Jacobs and P Rose, Removal of phenols from wastewater using soluble and immobilised fungal polyphenol oxidase. WISA-MTD Workshop on Preparation, Performance and Practice of permselective membranes, November 1994.

9. W Leukes, S Burton, E Jacobs and P Rose, Monitoring of a hollow fibre membrane-immobilised

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fungal enzyme production system. WISA-MTD Workshop on Preparation, Performance and Practice of permselective membranes, November 1994.

10. S G Burton, J P Hagemann, P T Kaye, and K W Wellington. Synthetic and complexometric studies of multidentate nitrogen ligand systems. SACI Frank Warren Conference, Bloemfontein, 1995.

11. S G Burton and S Kirchmann. Isolation and characterisation of polyphenol oxidase from tea (Camellia sinensis). Thirteenth Congress of the South African Biochemical Congress, Bloemfontein, 1995.

12. S G Burton, W Leukes, P D Rose, E Jacobs, R Sanderson and R Cross. A cytochemical method for localisation of lignin peroxidase in a Phanerochaete chrysosporium biofilm immobilised on a hollow fibre membrane. Thirteenth congress of the South African Biochemical Society, Bloemfontein, 1995.

13. S G Burton, R A Dorrington, M Gardner, S Kirchmann and C Hartley. Hydantoin-hydrolysing activity of microbial enzymes. Biotechnology for Africa ‘95, Pretoria, 1995.

14. W Leukes, S Burton, E Jacobs, R Sanderson and P Rose. Biofilm dynamics of Phanerochaete chrysosporium in a hollow fibre Bioreactor for sustained continuous production of lignin peroxidase. Biotechnology for Africa ‘95, Pretoria, 1995.

15. W. Edwards, R Bownes, W Leukes, S G Burton, E P Jacobs, R Sanderson and P Rose. Treatment of phenolic and cresylic industrial effluent using hollow-fibre membrane immobilised polyphenol oxidase. Biotechnology for Africa ‘95, Pretoria, 1995.

16. AECI Postgraduate Fellowship Meetings, 1990, 1991, 1992 and 1993. 17. (Research reports were read at each of these meetings) 18. Workshop on Microbial Biotransformations, conducted by Professor K Kieslich, National

Research Centre for Biotechnology, Braunschweig. (Bloemfontein, October 1995). 19. P Rose, S Burton, R Sanderson, E Jacobs, W Leukes and W Edwards. Membranes and

Biotechnology. Plenary lecture, 23rd SACI conference, Cape Town, 1996. 20. W Edwards, W Leukes, S Burton, E Jacobs, R Sanderson, and P Rose. Dephenolisation of

wastewater using immobilised polyphenol oxidase in a hollow-fibre membrane transverse-flow biological contacter. WISA 96, Port Elizabeth, 1996.

21. W Leukes, S Burton, W Edwards, E Jacobs, R Sanderson, and P Rose. A critical investigation into the use of Phanerochaete chrysosporium in a hollow-fibre bioreactor for the degradation of aromatic pollutants. WISA 96, Port Elizabeth, 1996.

22. C Hartley, M Gardner, S Kirchmann, S Burton and R Dorrington. Isolation and characterisation of hydantoinase-producing bacterial strains. Ninth Biennial Congress, South African Society for Microbiology, 1996.

23. S Kirchmann, C Hartley, M Gardner, R Dorrington and S Burton. Characterisation of microbial hydantoinase enzymes. Ninth Biennial Congress, South African Society for Microbiology, 1996.

24. S G Burton, 1997. Biotransformations research at Rhodes University. Biotech SA ‘97 Grahamstown.

25. A Boshoff, W Edwards, W Leukes, E Jacobs, S G Burton, and P D Rose. Application of membrane bioreactors for the biotransformation of organic pollutants in water. Biotech SA ‘97 and 14th SASBMB, Grahamstown, 1997.

26. R A Dorrington, K Buchanan, C Hartley, and S G Burton. 1997. The hydantoinase enzyme system: Diverse enzymes with a common biocatalytic application. 14th SASBMB, Grahamstown, 1997.

27. Carol J Hartley, Stephanie G Burton, and Rosemary A Dorrington. Factors affecting hydantoin-hydrolysing activity of Burkholderia cepacia RUO-R. Biotech SA ‘97 and 14th SASBMB, Grahamstown, 1997.

28. D R Ryan, W D Leukes and S G Burton. Biodegradation of cresol by Trametes versicolor as a function of microbial activity and chemical dose. Biotech SA ‘97 and 14th SASBMB, Grahamstown, 1997.

29. D R Ryan, W D Leukes, W Edwards, E P Jacobs, P Rose and S G Burton. Differentiation within a biofilm of Trametes versicolor immobilised on capillary membranes in transverse flow module. Biotech SA ‘97 and 14th SASBMB, Grahamstown, 1997.

30. Ingrid M Russell and Stephanie G Burton. Development of an immobilised-enzyme bioprobe for the detection of phenolic pollutants in water. Biotech SA ‘97 and 14th SASBMB, Grahamstown, 1997.

31. Kirsten Buchanan, Stephanie G Burton and Rosemary A Dorrington. An investigation of the factors affecting hydantoin-hydrolysing biocatalytic activity of Pseudomonas putida strain RUKM3s in resting cell reactions. Biotech SA ‘97 and 14th SASBMB, Grahamstown, 1997.

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32. Tina Russell, Winston Leukes and Stephanie Burton. An investigation of the biocatalytic potential of isolated polyphenol oxidase and fungal biomass from Neurospora crassa in the bioremediation of phenol-polluted water. Biotech SA ‘97, Grahamstown, 1997.

33. S G Burton and S Kirchmann. Polyphenol oxidase from tea (Camellia sinensis). Development of Extraction and Purification Techniques. 14th SASBMB, Grahamstown, 1997.

34. V Phehane, C Hartley, S Kirchmann, R A Dorrington, and S G Burton. The search for thermostable hydantoinases. Biotech SA ‘97, Grahamstown, 1997.

35. W Edwards, W D Leukes, E Jacobs, R Sanderson, S G Burton, and P D Rose. Chitosan-coated hollow-fibre membranes for phenolic effluent bioremediation. Biotech SA ‘97, Grahamstown, 1997.

36. W Leukes, M H Burton and S G Burton. Simulation of process conditions for p-cresol degradation using a membrane gradostat reactor with immobilised Phanerochaete chrysosporium. Biotech SA ‘97, Grahamstown, 1997.

37. Winston D Leukes, Ed P Jacobs, Ron D Sanderson, Stephanie G Burton, and Peter D Rose.

Biofilm dynamics of Phanerochaete chrysosporium in a membrane gradostat reactor. Biotech SA ‘97, Grahamstown, 1997.

38. Winston D Leukes, Ed P Jacobs, Ron D Sanderson, Stephanie G Burton, and Peter D Rose.

Evaluation of the use of a membrane gradostat reactor for the degradation of certain aromatic pollutants. Biotech SA ‘97, Grahamstown 1997.

39. S Kirchmann, R A Dorrington and S G Burton. Preliminary characterisation of hydantoinases from two local microbial strains. Biotech SA ‘97, Grahamstown, 1997.

40. S G Burton, W Leukes, W Edwards, A Boshoff, I Russell, T Russell, D Ryan, P Rose,E Jacobs, and R Sanderson. Biotransformations using membrane bioreactor technology 2nd WISA-MTD Workshop, October 1997.

41. A Boshoff, W Edwards, S G Burton. Application of membrane-immobilised polyphenol oxidase for the biotransformation of phenolic pollutants. 2nd WISA-MTD Workshop, October 1997.

42. W Edwards, W D Leukes, E P Jacobs, R Sanderson, P D Rose and S G Burton. The Influence of Diffusion Effects on Enzyme-Substrate Contact in a Capillary-Membrane Bioreactor. 2nd WISA-MTD Workshop, October 1997.

43. K Russell, W Leukes and S Burton. The biocatalytic potential of membrane-immobilised polyphenol oxidase and fungal biomass from Neurospora crassa in the biotransformation of phenols. 2nd WISA-MTD Workshop, October 1997.

44. Ingrid M Russell and Stephanie G Burton. The development of an immobilised-enzyme bioprobe for the detection of phenolic pollutants in water. 2nd WISA-MTD Workshop, October 1997.

45. D R Ryan, W D Leukes, W Edwards, E Jacobs, R Sanderson and S G Burton. The removal of aromatic compounds from solution by the fungus Trametes versicolor immobilised in a novel membrane bioreactor. 2nd WISA-MTD Workshop, October 1997.

46. W Edwards, W Leukes, E Jacobs, R Sanderson, P Rose and S Burton. Upscale of a transverse-flow capillary membrane module to a demonstration size unit for the treatment of phenol-containing industrial effluent. Water Institute of South Africa, Biennial conference, Cape Town, May 1998.

47. I M Russell and S G Burton. The development of an immobilised-enzyme bioprobe for detection of phenolic pollutants in water. Water Institute of South Africa, Biennial conference, Cape Town, May 1998.

48. A Boshoff and S G Burton. Application of membrane-immobilised polyphenol oxidase for the biotransformation of phenolic pollutants. Water Institute of South Africa, Biennial conference, Cape Town, May 1998.

49. A K Russell, W Leukes, and S G Burton. The biocatalytic potential of fungal biomass and membrane-immobilised polyphenol oxidase from Neurospora crassa in the biotransformation of phenols. Water Institute of South Africa, Biennial conference, Cape Town, May 1998.

50. D R Ryan, W D Leukes, E P Jacobs, R D Sanderson and S G Burton. Bioremediation of waste waters polluted with aromatic compounds by the white-rot fungus Trametes versicolor. Water Institute of South Africa, Biennial conference, Cape Town, May 1998.

51. C J Hartley, S G Burton and R A Dorrington. Regulation of the hydantoin hydrolysing enzyme system in Agrobacterium tumefaciens RUO-R. 10th Biennial Congress of the SA Society for Microbiology, July 1998.

52. G F Matcher, S G Burton and R A Dorrington. Characterisation of the hydantoinase and N-carbamyl amino acid amidohydrolase enzymes from Pseudomonas putida strain RUKM3s. 10th Biennial Congress of the SA Society for Microbiology, July 1998.

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53. C J Hartley, S G Burton, R A Dorrington. Utilisation of 5-fluorouracil to generate Agrobacterium tumefaciens RUO-R mutants with altered hydantoin-hydrolysing activity. 10th Biennial Congress of the SA Society for Microbiology, July 1998.

54. C J Hartley, S G Burton and R A Dorrington. Nitrogen repression of hydantoinase and N-carbamylamino acid amidohydrolase activity in Agrobacterium tumefaciens RUO-R: Evidence for multiple regulatory pathways. 10th Biennial Congress of the SA Society for Microbiology, July 1998.

55. A Boshoff and S G Burton. The effect of polyphenol oxidase immobilisation on the biotransformation of phenolic pollutants. 15th symposium, South African Society for Biochemistry and Molecular Biology, Potchefstroom, September 1998.

56. S G Burton, R A Dorrington, S Kirchmann, C Hartley, G Matcher, V Phehane and Z Skepu. Exploiting the hydrolysing activity of enzymes from hydantoinase-producing bacteria. 15th symposium, SASBMB, Potchefstroom, September 1998.

57. S Kirchmann, R Dorrington and S Burton. Characterisation and application of bacterial hydantoinases. 15th symposium, SASBMB, Potchefstroom, September 1998.

58. C J Hartley, S G Burton and R A Dorrington. Regulation of hydantoinase and N-carbamyl amino acid amidohydrolase enzymes in bacterial strain RU-OR. 15th symposium, SASBMB, Potchefstroom, September 1998.

59. Z Skepu and S G Burton. Production of enantiomerically pure products using the hydantoin-hydrolysing enzymes of strain RU-KM3s. 15th symposium, SASBMB, Potchefstroom, September 1998.

60. P Nganwa and S G Burton. Detection and characterisation of enzymes in sorghum plants for the enhancement of sorghum utilisation. 15th symposium, SASBMB, Potchefstroom, September 1998.

61. S G Burton. The future of Biotechnology in South Africa. FRD Biotechnology Workshop, August, 1998.

62. S G Burton. The way forward: Biochemical Processes. Report back to FRD Biotechnology Task team meeting, September 1998.

63. A Boshoff, M H Burton, S Burton. The effects of membrane immobilisation on the activity of polyphenol oxidase. 3rd WISA-MTD workshop, Drakensberg, 1999

64. A Luke and S Burton. Application of Neurospora crassa biofilms in capillary membrane bioreactors for phenol bioremediation. 3rd WISA-MTD workshop, Drakensberg, 1999

65. R D Ryan, W D Leukes, W Edwards, E P Jacobs, R Sanderson and S G Burton. Bioremediation of aromatic pollutants by the white-rot fungus Trametes versicolor immobilised in a transverse flow capillary membrane bioreactor. 3rd WISA-MTD workshop, Drakensberg, 1999

66. C Muzariri, S Burton, J Read, H Skilling, J Wood. Vitamin B1 determination in rural fermented sorghum and millet foods by reversed phase HPLC. BIOY2K, Grahamstown, 2000.

67. A. Boshoff, M H Burton and S.G. Burton. The effects of membrane immobilization on the activity of polyphenol oxidase. BIOY2K, Grahamstown, 2000.

68. IM Foster, SG Burton and RA Dorrington. Biochemical characterisation of hydantoin-hydrolysing enzyme systems in Agrobacterium tumefaciens RU-ORPN1 for utilisation in production of enantiomerically pure amino acids and related compounds. BIOY2K, Grahamstown, 2000

69. K.Koteshwar and S.G Burton . Transformation of organic compounds in different industrial wastes by using a novel peroxidase. BIOY2K, Grahamstown, 2000

70. S G Burton and T. L. Notshe Investigation into the bioconversion of constituents in olive waste effluents to produce valuable chemical compounds. BIOY2K, Grahamstown, 2000

71. AK Luke and SG Burton. Application of N.crassa biofilms in capillary membrane bioreactors for phenol bioremediation. BIOY2K, Grahamstown, 2000

72. S Kirchmann, T Clifford and S G Burton. Method development for the characterisation, application and localisation of bacterial hydantoinases. BIOY2K, Grahamstown, 2000

73. G Matcher, R Dorrington and S Burton. Novel hydantoinase and NCAAH enzyme activity in Pseudomonas sp. Strain RU-KM3s. SASM conference, Bloemfontein, 2002.

74. Brown S A, Burton S G and Cowan D A. Patterns of microbial diversity in the Cape Floral region: An investigation of proteiod rhizospheres in Leucadendron xanthoconus. SASM conference, Bloemfontein, 2002.

75. Biocatalysis, Biotransformations and Bioremediation. Presentation for Chemical Engineering Day, Cape Town, Feb 2002

76. S Burton, D Cowan and J Woodley. Towards the perfect Biocatalyst. Cape Biotech 2002, Cape Town, 2002.

77. van Schalkwyk A, Garcin C, Burton S G, Cowan D A. UV-mutagenesis as a means of improving

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the biotransformation potential of bacteria isolated from olive oil wastewater. SASBMB National conference, Pretoria, 2003.

78. Wittwer P E, Burton S G, Rashamuse K J and Cowan DA. Enzymology of Ferulic Acid Recovery. SASBMB National conference, Pretoria, 2003

79. Meyer Q, Smith J J, Harrison S T L, Burton S G, Soloman M and Cowan DA. 80. Recovering novel genes from environmental DNA via gene specific PCR. SASBMB National

conference, Pretoria, 2003 81. Chigorimbo-Tsikiwa N T L and 82. Burton S G,. Biotransformations of ferulic acid to produce value added compounds. SASBMB

National conference, Pretoria, 2003 83. Dürr R, Cowan D A, Syldatk C and Burton S G. Biodiversity of hydantoin cleaving enzymes .

SASBMB National conference, Pretoria, 2003. 84. M.B. Ting and S.G. Burton: Oxidation of Isoeugenol, Ferullic Acid and Resveratrol with a

phenol oxidase: A view towards production of pharmaceutically active compounds from natural sources (SASBMB 18th Congress, Molecules of Life UP-North 6-9 July 2003, University of Pretoria, Poster presentation)

85. C Garcin, S Burton, D Cowan. Identification, extraction and quantification of polyphenolic antioxidants from olive fermentation brines. SASBMB National conference, Pretoria, 2003.

86. Bulawayo BT, Dorrington, R and Burton SG. Amino acid production by hydantoinases of Pseudomonas putida, RUKM3s and Covalent Immobilisation in Eupergit® C for enhanced biocatalytic activity. SASBMB National conference, Pretoria, 2003.

87. Gwynneth F Matcher, Sally-Ann Clark, Stephanie G Burton and Rosemary A Dorrington . Mutational analysis to identify the structural genes encoding the hydantoin-hydrolyzing enzyme system in Pseudomonas putida. SASBMB National conference, Pretoria, 2003.

88. C.J. Vengadajellum, S.T.L. Harrison; D.A. Cowan, M.S. Solomon, S.G. Burton. Biosolubilisation of low rank coal: a novel route to value-added compounds. SASBMB National conference, Pretoria, 2003.

89. van Schalkwyk A, Garcin C, Burton S G, Cowan D A. UV-mutagenesis as a means of improving the biotransformation potential of bacteria isolated from olive oil wastewater. Cape Biotech, 2003.

90. Wittwer P E, Burton S G, Rashamuse K J and Cowan DA. Enzymology of Ferulic Acid Recovery. Cape Biotech, 2003.

91. Meyer Q, Smith J J, Harrison S T L, Burton S G, Soloman M and Cowan DA. Recovering novel genes from environmental DNA via gene specific PCR. Cape Biotech, 2003 91. Chigorimbo-Tsikiwa N T L and Burton S G,. Biotransformations of ferulic acid to produce value

added compounds. Cape Biotech, 2003 92. C Garcin, S Burton, D Cowan. Identification, extraction and quantification of

polyphenolic antioxidants from olive fermentation brines. Cape Biotech, 2003 93. C.J. Vengadajellum, S.T.L. Harrison; D.A. Cowan, M.S. Solomon, S.G. Burton.

Biosolubilisation of low rank coal: a novel route to value-added compounds. Cape Biotech, 2003

94. C. Garcin and S. Burton. Selective recovery of low moleular weight phenolic antioxidants from olive production wastewaters using membrane-assisted liquid extraction. Water Institute of South Africa (WISA) Biennial conference and exhibition, 2004, Cape Town, South Africa

95. LM Rohr, N Mashapu, SG Burton and DA Cowan. Comparative phylogenetics of the microbial population in a winery waste-impacted wetland. 14th Biennial Congress of the South African Society for Microbiology 9-12 April 2006 .

96. Development of biocatalytic applications of a novel thermostable nitrile hydratase (nhase). I Chiyanzu, D Cowan and S Burton. (SASBMB conference, July 2006)

97. Goodwin, C M, Le Roes-Hill, M and Burton, S G. Novel laccases from unique strains for use as biocatalysts to produce useful products. Bio 08, Grahamstown, South Africa.

98. Maele, K, Le Roes-Hill, M and Burton, S G. Rational screening method for the isolation of novel oxidizing enzymes with properties suitable for industrial biocatalysis applications. Bio 08, Grahamstown, South Africa.

99. Davids, N, Le Roes-Hill, M and Burton, S G. Screening new actinomycetes for oxidative enzymes with potential application in biocatalytic production of antioxidants. Bio 08, Grahamstown, South Africa.

100. Le Roes-Hill, M, Maele, K, Rohland, J, Bull, A, Cowan, D and Burton, S. Exploiting actinomycete biodiversity in the search for an ideal oxidase biocatalyst. Bio 08, Grahamstown, South Africa.

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101. Mupure, C H, Horne, K and Burton S G. 2008. ' Recovery of value added products from agro-industrial wastewaters'. Bio 08, Grahamstown, South Africa

102. Burton, S, F, Davids, L and Zwane, E. 2008. Investigation of the antioxidant activity of phenolic compounds in cell culture systems. Bio 08, Grahamstown, South Africa

103. Pather-Elias, S, Burton, S. 2008. "Compilation of a Database for the Detection of Mercaptans in South African Wine". Bio 08, Grahamstown, South Africa

104. Gervas Y. Murindamombe and Stephanie G. Burton. 2008. “Health for Purpose” in Wetlands Treating Waste Streams: Monitoring Enzyme Activities. Bio 08, Grahamstown, South Africa.

105. Olaofe, O.A., Burton, S.G., Cowan, D.A. and Harrison, S.T.L. 2008. Towards highly efficient recombinant amidase production through bioreactor optimisation. Bio 08, Grahamstown, South Africa.

106. Recovery of value added products from agro-industrial wastewaters, S.G. Burton, K. Horne and C.H. Mupure, Bio 08 Conference, Grahamstown, January 2008

107. Kerry Horne, Andrew Nel, Don Cowan, Kirstin Eley and Stephanie Burton, Fermentation of lignocellulosic waste by a novel Bacillus licheniformis isolate, Cape Biotech Forum, November 2008

108. Goodwin, C.M., le Roes-Hill, M., and S. G. Burton. 2008. Novel laccases from unique strains for use as biocatalysts to produce useful products. Page 102. BIO-08 Conference, Rhodes University.

109. Goodwin, C.M., le Roes-Hill, M., and S. G. Burton. 2008. Optimisation of laccase production by Micromonospora sp. strain MB for biocatalysis applications. Page 204. Extremophiles 2008, South Africa.

110. Goodwin, C.M., le Roes-Hill, M., and S. G. Burton. 2008. Optimisation of laccase production by Micromonospora sp. strain 044 30-1 for biocatalysis applications. Page SP27. Cape Biotechnology Forum, South Africa.

111. Davids, N, le Roes Hill, M, Burton, SG.(2008) Screening new actinomycetes for oxidative enzymes with potential application in biocatalytic production of antioxidants. Bio 08, Grahamstown, South Africa

112.Davids, N, le Roes Hill, M, Burton, SG.(2008) Deep-sea actinomycetes strains having the ability to degrade Crystal Violet.A potential source of novel oxidative enzymes for industrial application. Cape Biotech Forum. Somerset West, South Africa.

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REFEREES Professor Frances H. Arnold Dick and Barbara Dickinson Professor Division of Chemistry & Chemical Engineering 210-41 California institute of Technology 91125 FAX: 626-568-8743 Tel: 626-395-4162 Email: frances@cheme.caltech.edu Professor Peter Clayton Deputy Vice-Chancellor (Research) Rhodes University P Box 91 Grahamstown 6140 Phone: +27 46 6038055 Fax: +27 46 622 8822 Email: p.clayton@ru.ac.za Dr Dr C M de la Rey Chief Executive Officer Council on Higher Education P O Box 13354, The Tramshed, 0126 tel: +27 12 392 9111 fax: +27 12 392 9117 delarey.c@che.ac.za Professor Sakkie Pretorius Australian Wine Research Institute PO Box 197 Glen Osmond, Adelaide Australia SA 5064 Phone (+ 61 8) 8303 6610 Fax: (+61 8 ) 8303-6609 Sakkie.Pretorius@awri.com.au

Professor Roberta Farrell Department of Microbiology School of Biological Sciences Faculty of Science & Engineering University of Waikato Private Bag 3105 Hamilton New Zealand Phone: +64 7 838 4704 Fax: +64 7 838 4218 Email: r.farrell@waikato.ac.nz Professor Greg Blatch Professor and Glaxo-Welcome Scholar Department of Biochemistry, Microbiology and Biotechnology Rhodes University Grahamstown 6140

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South Arica Phone: +27 46 6038262 Fax: +27 476 6223984 Email: G.blatch@ru.ac.za Professor Sir Wil Konings Visiting Professor Department of Biochemistry, Stellenbosch University and previously (until 2006) Professor Department of Molecular Microbiology University of Groningen Haren, The Netherlands Email: wnkonings@tiscali.nl