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Personal information

First name(s) / Surname(s) Elisa Andreozzi Address(es) via Ascensione 14, 62010, Mogliano (MC), Italy

Telephone(s) +393275425252 +393386318036

E-mail elis.83@libero.it

Nationality Italian

Date of birth 11/02/1983

Gender Female

Desired employment / Occupational field

Biologist

Education and training

Dates

November 2011 – October 2014

Title of qualification awarded PhD Dissertation: ‘Study of anti-biofilm activity of chemicals in order to remove Legionella pneumophila in water systems’

Principal subjects/occupational skills covered

Research on disinfection techniques for the eradication of Legionella bacteria in water systems by identifying chemical substances able to inhibit the formation of biofilms. • Legionella spp isolation and identification from water sample. • Investigation of biofilm formation ability by L. pneumophila and other strains and biofilm

quantification. • Study of biofilm structure by fluorescence, confocal and electron microscopy, using static

and dynamic models (flow chambers). • Study of chemicals cytotoxicity, antimicrobial activity (cytofluorimetry) and antibiofilm

activity. • Study of changes in gene expression in the planktonic and adhering phase of Legionella

(real time PCR). Support work in lab practice organization. Organization and conduction of seminars for students in Biological Science and Nutrition Science.

Name and type of organisation providing education and training

Urbino University Department of Biomolecular Sciences, Section of Toxicological Hygienic and Environmental Sciences (Urbino, Italy)

Dates

June – July 2014

Title of qualification awarded Workshop

Principal subjects/occupational skills covered

Water samples analysis by epifluorescence microscopy and digital image analysis using Image J program. Nucleic acid extraction and microorganisms identification by PCR; Legionella spp identification and quantification by real-time PCR. Study of L. pneumophila infection ability on amoebas and macrophages by CFU count and fluorescence microscopy.

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Name and type of organisation providing education and training

Helmholtz Centre for Infection Research (HZI) (Braunschweig, Germany)

Dates September-December 2013

Title of qualification awarded Internship

Principal subjects/occupational skills covered

Investigation of microorganisms in water by fluorescence microscopy techniques (probes, antibodies). Investigation of bacterial biofilm formation using confocal and fluorescence microscopy.

Name and type of organisation providing education and training

Helmholtz Centre for Infection Research (HZI) (Braunschweig, Germany)

Dates

October-November 2011

Title of qualification awarded Trainee

Principal subjects/occupational skills covered

Microbiological analysis of water (mineral, surface and wastewater)

Name and type of organisation providing education and training

ARPAM (Agenzia Regionale per la Protezione Ambientale delle Marche – Regional Agency for environmental protection of the Marches), public sector

Dates

July 2010

Title of qualification awarded Qualification to work as a Biologist in Italy

Principal subjects/occupational skills covered

State qualifying examination for Biologist (A section)

Name and type of organisation providing education and training

Urbino University (Italy)

Dates

2006-2009

Title of qualification awarded

Second level degree in Cellular and Molecular Biology Final score: 110/110 cum laude Dissertation: ‘Identification of Aeromonas spp strain isolated from clinical samples, food and water, and investigation of virulence factors’

Principal subjects/occupational skills covered

Molecular Biology, Cell Physiology, Pharmacology, Molecular Pathology, Embryology, Genetic Engineering, Bioinorganic Chemistry, Analysis of Organic Molecules by Physical Methods, Cytochemistry, Histochemistry, Cytometry, Applied Hygiene

Name and type of organisation providing education and training

Urbino University (Italy)

Dates 2002-2006

Title of qualification awarded First level degree in Biology Final score: 110/110 cum laude Dissertation: Bioremediation: ‘N-butyl-acetate degradation by autocthonous microorganisms isolated from a firm producing rollers covered by elastomers’

Principal subjects/occupational skills covered

Chemistry, Physics, Maths, Biochemistry, Cytology, Histology, Zoology, Botanic, Genetic, Microbiology, Virology, Hygiene

Name and type of organisation providing education and training

Urbino University (Italy)

Dates

1997-2002

Title of qualification awarded Classical education Final score: 97/100

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Principal subjects/occupational skills covered

Italian Literature, Latin, Greek, History, Philosophy, Art History, English, Maths, Physics, Chemistry, Biology

Name and type of organisation providing education and training

Liceo Clasico ‘G. Leopardi’ (Macerata, Italy)

Personal skills and competences

Mother tongue(s) Italian

Other language(s) English Self-assessment Understanding Speaking Writing European level (*) Listening Reading Spoken interaction Spoken production

English C1 C1 C1 C1 C1

(*)Common European Framework of Reference for Languages

Technical skills and competences

Optical and fluorescence/immunofluorescence microscopy. DNA, RNA, protein extraction, PCR, real time PCR, western blot. Microbiology techniques for the isolation and characterization of bacteria. Study and identification of microorganisms using biochemical and molecular methods. Cell cultures (INT 407, HEp-2, HeLa, Vero, Caco-2 cell lines and amoeba): adhesivity, invasivity, cytotoxicity, viability assay.

Computer skills and competences Good competences in working with Microsoft Office and using the Web.

Social skills and competences Teamwork skills acquired working in research teams at several Universities. Good organization skills and tasks management ability. Inclination for socialization and ability to adapt to multicultural environments.

Other skills and competences • Occasional clerical and waitress jobs. • Assistant instructor of artistic gymnastic training guys (6-18 years old) for regional and

national amateur competitions and for gym exhibitions.

Driving licence Italian B Licence

Additional information Erasmus Project from January to June 2008 at the University of Kent (Canterbury, England), working in a microbiology lab with prions of yeast cells: ‘Elimination of the [PIN+] prion from yeast by guanidine salts’ • Andreozzi E, Cesare A, Sabatini L, Chessa E, Sisti D, Rocchi M, Citterio B, 2014. Role

of biofilm in the protection of the replicative form of Legionella pneumophila, Current Microbiology 69(6):769-74.

Publications

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Elisa Andreozzi