24Feb2015EndofDayShutdown

CyTOF2EndoftheDayShutdown(SoftwareVersion6.0.626)

A. Washingsamplelines(Afteryourlastsamplehasbeenrun)1. FollowthefinalwashproceduresoutlinedintheSampleAcquisitionSOP.2. Oncebackgroundlevelsareacceptable,we’rereadytoshutdown.

B. TurningoffthePlasma

1. Clickthe“About”TabontheCyTOFhomewindow

a. Select“Login”intoadministratoruseraccount1. Enablesaccesstothe“ControlPanel”2. Administrator/donuts

2. Clickthe“ControlPanel”TabintheCyTOFMainMenutoopenthecontrolpanel3. Clickthe“Plasma”TabtoopenthePlasmacontrol4. Click“StopPlasma”

a. Shutdowntakesabout5-10minutes

24Feb2015EndofDayShutdown

b. TheSyringePump,ChillerandHeaterwillshutoffaspartoftheshutdownprocedure

5. WaitforthepromptindicatingasuccessfulshutdownbeforeproceedingtoPartC

C. TurnoftheArgonGasflowontheTank1. Ifgasisbeingdrawnfromacylinder,pleaseshutoffgasflowattheCylinder2. IfgasisbeingdrawnfromtheArgondewar,noactionisrequired.

24Feb2015EndofDayShutdown

D. RemovetheNebulizerfromtheNebulizerport

E. Disassemblethenebulizer1. RemovetheSampleCapillaryfromtheNebulizer2. PlacetheFlangelessfixtureinitshomeunderneaththeheater

3. DisconnectthegaslinefromtheNebulizera. UnscrewtheSwagelokfromtheNebulizersidebeingcarefultocatchtheferruleandtheO-ring

1. RecommendthatyouholdtheNebulizerupsidedowntocatchallthreepiecesoftheattachmentassembly

a. WhiteNylonFerrule

24Feb2015EndofDayShutdown

b. BlackO-ringc. WhiteSwageloknut

b. RemovetheNebulizerc. ReassembletheSwagelokfixturewiththeferruleandO-ringembeddedontothegaslineandstorethegaslineonthehousingnexttotheHeatercage

d. FilltheNebulizerwiththemilliQwaterusingasyringeattachedtotheNebulizersidearmandtheNebulizersampleline

e. PlacethenebulizerinitsStorageCasefilledwithMilliQwaterandsoakovernightoruntilnextuse

24Feb2015EndofDayShutdown

F. ClosetheCyTOFsoftwarepackageG. Doublecheckthatyouperformeda“FACSDataCheck-in”H. Logoutofyourwindowsaccounttostopthatday’saccounting

LeaveallothercomponentsontheCyTOFrunningindefinitely