DEPARTMENT OF BIOTECHNOLOGY - Rajah Serfoji ... culture technique - Pour plate, Spread plate and Streak plate methods. 6. Biochemical characterization of selected bacteria. 7. Measurement

DEPARTMENT OF BIOTECHNOLOGY

SYLLABUS FOR

B.Sc., (BIOTECHNOLOGY)

Applicable to the Candidates admitted from the Academic year

2015-2016 onwards

RAJAH SERFOJI GOVERNMENT COLLEGE (AUTONOMOUS) THANJAVUR- 613 005

RAJAH SERFOJI GOVT. COLLEGE (AUTONOMOUS), THANJAVUR-5

COURSE STRUCTURE FOR B.Sc.,

SUBJECT: BIOTECHNOLOGY

(Applicable to the Candidates admitted from the academic year 2015-2016 onwards)

Part Code Course Title Hrs Marks

Total Credit IE WE

I-SEMESTER

I RR1T1 LT PART-I Tamil-I 6 25 75 100 3

II RR1E1 LE PART-II English-I 6 25 75 100 3

III RR1BT1 CC1 General Microbiology 6 25 75 100 5

III RR2BTP1 CC3 Major Practical-I 3 - - -

III RR1AZ1 Allied 1 Allied Zoology-I 4 25 75 100 4

III RR2AZP Allied 3 Allied Zoology Practical 3 - - -

IV RR1VE VE Value Education 2 25 75 100 2

TOTAL 30 500 17

II-SEMESTER

I RR2T2 LT PART-I Tamil-II 6 25 75 100 3

II RR2E2 LE PART-II English-II 6 25 75 100 3

III RR2BT2 CC2 Cell and Molecular Biology 6 25 75 100 5

III RR2BTP1 CC3 Major Practical-I 3 40 60 100 4

III RR2AZ2 Allied 2 Allied Zoology-II 4 25 75 100 4

III RR2AZP Allied 3 Allied Zoology Practical 3 40 60 100 4

IV RR2ES ES Environmental Studies 2 25 75 100 2

TOTAL 30 700 25

RAJAH SERFOJI GOVT. COLLEGE (AUTONOMOUS), THANJAVUR - 613005



(Applicable to the candidates admitted from the academic year 2015-2016 onwards)


Total Credit IA AE

III-SEMESTER

I RR3T3 LT PART-I Tamil-III 6 25 75 100 3

II RR3E3 LE PART-II English-III 6 25 75 100 3

III RR3BT3 CC4 Molecular Genetics 6 25 75 100 5

III RR4BTP2 CC6 Major Practical-II 3 - - -

III RR3ABC1 Allied 4 Allied Biochemistry-I 4 25 75 100 4

III RR4ABCP Allied 6 Allied Biochemistry Practical 3 - - -

IV RR3SB1 SB1 Skill Based-I 2 25 75 100 2

TOTAL 30 500 17

IV-SEMESTER

I RR4T4 LT PART-I Tamil-IV 6 25 75 100 3

II RR4E4 LE PART-II English-IV 6 25 75 100 3

III RR4BT4 CC5 rDNA Technology 6 25 75 100 5

III RR4BTP2 CC6 Major Practical-II 3 40 60 100 4

III RR4ABC2 Allied 5 Allied Biochemistry-II 4 25 75 100 4

III RR4ABCP Allied 6 Allied Biochemistry Practical 3 40 60 100 4

IV RR4SB2 SB2 Skill Based-II 2 25 75 100 2

Total 30 700 25

RAJAH SERFOJI GOVT. COLLEGE (AUTONOMOUS), THANJAVUR - 613005



(Applicable to the candidates admitted from the academic year 2015-2016 onwards)


Total Credit IA AE

V-SEMESTER

III RR5BT5 CC7 Animal Biotechnology 6 25 75 100 5

III RR5BT6 CC8 Plant Biotechnology 6 25 75 100 5

III RR5BTP3 CC9 Major Practical-III 3 40 60 100 4

III RR5BTEL1 MEC1 Bioinformatics 5 25 75 100 4

III RR5BTEL2 MEC2 Immunotechnology 4 25 75 100 4

III RR5SELO1 NMEC1 Biostatistics 4 25 75 100 4

IV RR5SB3 SB3 Skill Based-III 2 25 75 100 2

TOTAL 30 700 28

VI-SEMESTER

III RR6BT7 CC10 Bioprocess Technology 6 25 75 100 5

III RR6BT8 CC11 Enzyme Technology 6 25 75 100 5

III RR6BT9 CC12 Environmental Biotechnology 5 25 75 100 4

III RR6BTP4 CC13 Major Practical-IV 3 40 60 100 4

III RR6BTEL3 MEC3 Biotechnology and Human Health 4 25 75 100 4

III RR6BCELO2 NMEC2 Nutrition & Health Sciences 4 25 75 100 4

V RR6GS GS Gender Studies 2 25 75 100 1

V Ext. Act NSS/ NCC/ Sports/ Red Cross - - - - 1

TOTAL 30 700 28

GRAND TOTAL 3800 140

PART COURSE PAPERS CREDITS

PART-I TAMIL 4 x 3 12

PART-II ENGLISH 4 x 3 12

PART-III

CORE 8 x 5 = 40

5 x 4 = 20

60

ELECTIVES 3 x 4 12

ALLIED 6 x 4 24

NON-MAJOR 2 x 4 8

PART-IV ES, VE 2 x 2 4

SKILL BASED 3 x 2 6

PART-V

GS 1 x 1 1

EXT.ACTIVITIES

1

TOTAL PAPERS 38 TOTAL CREDITS 140

Credits : 5

Hours/Week : 6

Medium of Instruction : English Code: RR1BT1

Semester : I

CC1- GENERAL MICROBIOLOGY

UNIT-I

History of microbiology - Basic principles in microscopy, Types of microscopes - light, dark,

phase contrast, fluorescent and electron microscope (TEM & SEM).

UNIT-II

A detailed account of general structure, growth and reproduction of Algae, Bacteria, Fungi and

Virus. Bacterial classification- three kingdom and five kingdom concept.

UNIT-III

Microbiological Media - types, preparation, techniques of pure culture, maintenance and

preservation, Staining - stains and types of staining.

UNIT-IV

Microbial Disease - Water borne disease (Cholera), Food borne disease (Typhoid), Air borne

(Pneumonia), Sexually transmitted disease (Syphilis), Vector borne disease (Malaria) - Viral

disease (Rabies, HIV) - Fungal disease (Candidiasis).

UNIT-V

Control of Microorganisms - Methods of sterilization, physical agents - chemical agents,

chemotherapeutic agents (Penicillin, Streptomycin & Tetracycline).

Text & Reference Books:

1. Prescott L.M, J.D. Harley. and D. A. Klein, (1999). Microbiology, McGraw Hill.

2. Pelczar M.J., Jr., E.C.S. Chang. and N.R. Krieg, (1993). Microbiology - Concepts and

Applications, McGraw Hill Company.

3. Ronald M. Atlas, (1987). Microbiology, Fundamentals and Applications, Prentice Hall.

4. Stainer, M. (1986). General Microbiology, McMillan Publishing Co.

Question Paper Pattern (Marks: 75) (Time: 3 Hours)

Part - A : Two Questions from each Unit (No choice) (10 x 2 = 20 Marks)

Part - B : Either or Questions (One pair from each Unit) (5 x 5 = 25 Marks)

Part - C : Three out of Five (One from each Unit) (3 x 10 = 30 Marks)

Credits : 4

Hours/Week : 3

Medium of Instruction : English Code: RR2BTP1

Semester : II

MAJOR PRACTICAL - I

CC3 - LAB IN GENERAL MICROBIOLOGY AND CELL & MOLECULAR BIOLOGY

Lab in Microbiology

1. Laboratory rules and regulations of Microbiology.

2. Microscope and its functions.

3. Media preparation and sterilization.

4. Enumeration of microorganism from soil, water and spoiled food - serial dilution technique.

5. Pure culture technique - Pour plate, Spread plate and Streak plate methods.

6. Biochemical characterization of selected bacteria.

7. Measurement of growth of bacteria.

8. Staining of bacteria - simple, Gram’s, spore and capsule.

9. Fungal staining - Wet Mount technique.

Lab in Cell and Molecular Biology

10. Microscopic identification of chromosome in Chironomous larvae.

11. Barr body identification in buccal smear cells.

12. Identification and characterization of different types of Blood cells.

13. Histo-chemical staining techniques.

14. Identification of various stages of cell division (mitosis and meiosis).

Credits : 5

Hours/Week : 6


Semester : II

CC2 - CELL AND MOLECULAR BIOLOGY

UNIT-I

Cell as a basic unit - Cell size and shape - cell theory - structure of prokaryotic and eukaryotic

cells.

UNIT-II

Cellular organization - plasma membrane, cell wall, their structural organization, transport of

nutrients, ions and macromolecules across the membranes. Cellular energy transactions - Role

of mitochondria and chloroplast. Cellular organelles (Cytosol, endoplasmic reticulum, nucleus,

cytoskeleton, ribosomes, vacuoles, peroxisomes and lysosome).

UNIT- III

Cell division (Eukaryotic and Prokaryotic) - mitosis, meiosis and cell cycle. DNA replication -

prokaryotes and eukaryotes.

UNIT-IV

Gene expression: Transcription in prokaryotes and eukaryotes post transcriptional

modifications. Genetic code. Translation in prokaryotes and eukaryotes post translational

modification.

UNIT-V

Regulation of gene expression - regulatory elements- sigma factor, promoter, operator,

attenuator. Operon concept- lac, trp, Si RNA.


1. De Robertis E. D. P. (2001). Cell and Molecular Biology, 8th

Edition. Blaze Publishers and

Distributors Pvt. Ltd., New Delhi.

2. De Roberties and De Roberties, (2001). Cell Biology, Blaze Publishers & Distributors Pvt.

Ltd., New Delhi.

3. Harvey F. Lodish, Arnold Berk, Paul Matsudaira, David Baltimore, S. Lawrence Zipursky.

and James Darnell. (1999). Molecular Cell Biology. 4th

Edition, W H Freeman and Co. (Sd).

4. James Darnet, Harvey Lodish. and David Baltimore. (1986). Molecular Cell Biology,

Scientific American Books Ins.





http://www.google.at/search?hl=de&tbo=p&tbm=bks&q=inauthor:%22Harvey+F.+Lodish%22

http://www.google.at/search?hl=de&tbo=p&tbm=bks&q=inauthor:%22Arnold+Berk%22

http://www.google.at/search?hl=de&tbo=p&tbm=bks&q=inauthor:%22Paul+Matsudaira%22

http://www.google.at/search?hl=de&tbo=p&tbm=bks&q=inauthor:%22David+Baltimore%22

http://www.google.at/search?hl=de&tbo=p&tbm=bks&q=inauthor:%22S.+Lawrence+Zipursky%22

http://www.google.at/search?hl=de&tbo=p&tbm=bks&q=inauthor:%22James+Darnell%22

Credits : 5

Hours/Week : 6


Semester : III

CC4 - MOLECULAR GENETICS

UNIT-I

Mendelian Principles, principles of population genetics - Hardy Weinberg law, Chromosome

structure and functions - Organization of chromosomes, specialized chromosomes, chromosome

abnormalities.

UNIT- II

Human Chromosomes - Pedigree analysis, chromosomal analysis - Epistasis - Linkage and

crossing over - three point cross -tetrad analysis- chromosome mapping- genome analysis.

UNIT-III Structure of gene - modern concept of gene, DNA as the genetic material (Griffith, Avery -

MacLeod - McCarty experiment) and RNA as a genetic material.

UNIT -IV

DNA structure and functions - Watson crick model, types of DNA, structure of RNA, types and

functions. Transposable elements.

UNIT-V

Viruses and their genetic system - Life cycles (lytic and lysogenic), RNA phages, Gene transfer

methods - Conjugation, Transformation, Transduction, Transfection.


1. David Freifelder. (1990). Essentials of Molecular Biology, Narosa Publishing House, New

Delhi.

2. Klug, W.S. and M.R. Cummings. (1997). Concepts of Genetics, Prentice Hall, NJ, USA.

3. Gardner E.J., Simmons, M.J, and Squstad, D.P. (1991). Principles of Genetics, John Wiley

and Sons, California.

4. Lewin, B. (2008). Genes IX, Jones and Bartlett, London.

5. Darnell, J.E., Baltimore, D. and Lodish, H.F. (1994). Molecular Cell Biology, Scientific

American Books, Inc., Virginia.





Credits : 4

Hours/Week : 3


Semester : IV

MAJOR PRACTICAL - II

CC6 - LAB IN MOLECULAR GENETICS & rDNA TECHNOLOGY

Lab in Molecular Genetics

1. Isolation of DNA.

2. Estimation of DNA.

3. Transformation in E. coli.

4. Replica plate technique.

5. Mutant isolation by gradient plate technique.

Lab in rDNA Technology

6. Preparation of plasmid DNA and genomic DNA from E. coli.

7. Preparation of genomic DNA from animals / human / plant.

8. Agarose gel electrophoresis of plasmid and genomic DNA.

9. PCR amplification.*

10. Southern blotting.*

11. Transformation of E. coli with plasmid DNA using CaCl2.*

* Practical by demonstration only.

Credits : 5

Hours/Week : 6


Semester : IV

CC5 - rDNA TECHNOLOGY

UNIT-I

Generation of DNA, cutting and joining of DNA molecules - exonucleases, endonucleases,

ligases. DNA modifying enzymes - methylase, alkaline phosphatase and topoisomerase.

UNIT-II

Vectors - Plasmids (pBR322, pUC18), Bacteriophage, Phagemids, Cosmids, Ti and Ri plasmids,

BAC, YAC, expression vectors, shuttle vectors.

UNIT-III

Transfer of DNA into Cells - transformation, CaCl2 mediated Ultra-sonication, Electroporation,

Micro-injection, Macro-injection, Particle bombardment system and Liposome mediated gene

transfer.

UNIT-IV

Cloning in E. coli, Bacilli and Yeast. Selection and screening of recombinants (antibiotics,

expression basis - GUS expression) Blotting techniques - Southern, Western and Northern

blot.

UNIT-V

Construction of genomic libraries and cDNA library, DNA sequencing methods - chemical

degradation, chain termination. Application of rDNA Technology in animals - Production of

Vaccine - Insulin.


1. Brown T.A. (2010). Gene Cloning and DNA Analysis: An Introduction, 6th

Edition, Wiley-

Blackwell.

2. Old, R.W and S.B. Primrose. (1996). Principles of Gene Manipulation: An Introduction to

Genetic Engineering, Blackwell Scientific Publications, Oxford.

3. Glover, DM. and B.D. Hames. (1995). DNA Cloning: A Practical Approach, IRL Press,

Oxford, Innis,

4. Persing, D.H., K T.F Smith, F.C. Teower and T.J. While. (1993). Diagnostic Molecular

Microbiology, ASM Press, Washington, D.C.





Credits : 5

Hours/Week : 6


Semester : V

CC7- ANIMAL BIOTECHNOLOGY

UNIT-I

Structure and organization of animal cells. Embryology - Gametogenesis and fertilization in

animals. In vitro fertilizations and embryo transfer, Stem cell culture.

UNIT-II

Animal cell culture: media formulations. Types of cell culture - primary cell culture, secondary

cell culture, cell transformation, cell lines, stem cell cultures, cell viability and cytotoxicity.

Cryopreservation.

UNIT-III

Construction of animal vectors - SV40, Adenovirus, Baculovirus. Transgenic animals production

and application. Hybridoma technology and Xenografting.

UNIT-IV

Gene therapy, mapping of human genome - RFLP and its applications, DNA finger printing and

foot printing.

UNIT-V

Ethical issues in animal biotechnology - use of antibodies and nucleic acids, problems in clinical

diagnosis of genetic disorders. Merits and demerits of transgenic animals.


1. Ramadas, P. (2008). Animal Biotechnology, MJP Publishers, Chennai.

2. Ignacimuthu. S. (1995). Basic Biotechnology, Tata McGraw Hill Publishers, New Delhi.

3. Butler, M. (Ed.). (1990). Mammalian Cell Biotechnology - A Practical Approach, Oxford

Univ. Press, Oxford.

4. Murray E.J. (Ed). (1991). Gene Transfer and Expression Protocols - Methods in Molecular

Biology, Vol.7. Humana Press, Totowa, NJ.

5. Ranga M.M. (2004). Animal Biotechnology, 2nd

Edition, Agrobios, India.





Credits : 5

Hours/Week : 6


Semester : V

CC8 - PLANT BIOTECHNOLOGY

UNIT-I Plant genome organization. Organization of chloroplast genome, organization of mitochondrial

genome, cytoplasmic male sterility. Genomic interaction - Protoplasmic fusion.

UNIT-II Plant tissue culture - sterilization of explants, media preparation and composition (MS), plant

growth hormones - auxin, cytokinins.

UNIT-III Embryogenesis, organogenesis (direct and indirect callus cultures, production of haploids and

cybrids. Plant hormones and phytochrome. Seed storage proteins.

UNIT-IV Molecular Biology of Plant - pathogen interactions (an overview) - Importance of RFLP in plant

Breeding - Ti plasmid vectors for plant transformation. Biochemistry and molecular biology of

nitrogen fixation in legumes by Rhizobium. Synthetic seed.

UNIT-V

Transgenic plants - pest resistant, herbicide resistant, disease resistant, drought tolerance, delay

fruit ripening, merits and demerits.


1. Chawla, H.S. (2009). Introduction to Plant Biotechnology, 3rd

Edition. New Delhi.

2. Ignachimuthu, S. (1995). Basic Biotechnology, Tata McGraw Hill Publishers, New Delhi.

3. Grierson, D. and S.N. Covey. (1988). Plant Molecular Biology, Blackie & Sons. Ltd.

4. Dubey, R.C. (1993). A Text Book of Biotechnology, 5th

Edition S. Chand Publishing Pvt.

Ltd., India.





https://www.schandpublishing.com/author-details/-r-c-dubey/145

Credits : 4

Hours/Week : 3


Semester : V

MAJOR PRACTICAL – III

CC9 - LAB IN ANIMAL BIOTECHNOLOGY, PLANT BIOTECHNOLOGY

AND IMMUNOTECHNOLOGY

Lab in Animal Biotechnology

1. Preparation of tissue culture medium.

2. Preparation of single cell suspension from spleen and thymus.

3. Cell counting and viability.

4. Cryopreservation and thawing.

Lab in Plant Biotechnology

1. Surface sterilization.

2. Micro propagation - Direct and Indirect Organogenesis.

3. Protoplast Isolation and culture.

4. Cytological examination of regenerated plants.

Lab in Immunotechnology

1. Blood film preparation and identification of cells.

2. Radial Immune Diffusion.

3. ELISA.

4. Immunodiagnostics - Widal test and Pregnancy test.

Credits : 4

Hours/Week : 5

Medium of Instruction : English Code: RR5BTEL1

Semester : V

MEC1 - BIOINFORMATICS

UNIT-I

Bioinformatics - Overview definition and history. Structure and chemical composition of nucleic

acids and proteins.

UNIT-II

Biological resource database - Protein and nucleic acid sequence databases (NCBI, EMBL,

GenBank, Swiss-Prot and PIR), Pattern and motif searches (BLOCKS, PRINTS). Structural,

classification, Alignment and analysis (SCOP, CATH, FSSP)

UNIT-III

Sequencing analysis - pairs wise comparison; Local (Smith and Waterman algorithm) and Global

(Needleman and Wunsch algorithm), BLAST, FASTA. Multiple sequence alignment.

UNIT-IV

Protein prediction - Secondary structure prediction - Chou-Fasman method, predicting 3D

structure - comparative (homology) modeling. Protein structure visualization tools - Rasmol,

Chime.

UNIT-V

Evolutionary analysis - distance based method, clustering based method - rooted and unrooted

tree - phylogenetic trees - PHYLIP.


1. Attwood, T.K and Parry-Smith. (2006). Introduction to Bioinformatics, 1st Edition, Pearson

Education, India.

2. David W. Mount. (2005). Bioinformatics sequence and Genome analysis, 2nd

Edition, CBS.

3. Andreas D., Baxevanis. B.F. and Francis Ouellette. (2005). Bioinformatics, 3rd

Edition, John

Willey & Sons.

4. Brayen Bergeron. (2003). Bioinformatics Computing, M.D. Pearson Education.





Credits : 4

Hours/week : 4


Semester : V

MEC2 - IMMUNOTECHNOLOGY

UNIT-I

Introduction - History and scope of Immunotechnology. Organs of the immune system: bone

marrow, spleen, lymph nodes, MALT.

UNIT-II

Types of immunity - Antigen-Antibody reactions. Antigen - types, immunoglobulins -

distribution and functions. Lymphoid tissues - ontogeny and physiology and immune system.

T & B Cells - receptors - activation and function.

UNIT-III Cellular interactions in immune response, Hypersensitivity reactions, HLA Tissue typing,

transplantation immunity, vaccine production.

UNIT-IV Immunodiagnostics - precipitation, agglutination, Widal test, pregnancy test.

Immunoblotting techniques - ELISA and FISH.

UNIT-V

Autoimmune disorders and immunology of infectious diseases including AIDS. Introduction

to tumor immunology - Immune evasion - Immune suppression. General introduction to

monoclonal antibodies and vaccines. Structure and functions of cytokines.


1. Kuby, J. (1997). Immunology, 3rd

Edition, W.H. Freeman and Co.

2. Roitt, I.M., J. Brostoff. and D.K. Male. (1993). Immunology, Gower Medical Publishing,

London.

3. Benzamini, E., G. Sunshine and Leskpwitz. (1996). Immunology - A Short Course, Willy

- Liss.

4. Gabrial Virellce. and Marcel Dekkar. (1993). Introduction to Medical Immunology, W.H

Freeman and Co.

5. Donald M. Weir. and John Steward, (1993). Immunology, 7th

Edition. ELBS, London.





Credits : 5

Hours/week : 6


Semester : VI

CC10 - BIOPROCESS TECHNOLOGY

UNIT-I Isolation, preservation and maintenance of industrially important microorganisms. Major classes

of commercial products using micro organisms.

UNIT-II

Basic principles of bioprocess - media formulation for various organisms sterilization methods -

batch and continuous sterilization system. Types of fermentation.

UNIT-III

Parts, designing, types of fermentor (batch, fed-batch and continuous bioreactors, Air - lift,

stirred tank, tower, fluidized bed, packed bed, pulsed and photo bioreactors) - Downstream

processing.

UNIT-IV

Bioprocess control and monitoring of temperature, pressure, agitation and aeration, pH,

computers in biocontrol.

UNIT-V

Industrial Production of Chemicals: Alcohol (Ethanol), Acids (Citric) Antibiotics (Penicillin),

Amino acids (Lysine), Single Cell Protein (Algae / Fungi) and their applications.

Text & Reference Books: 1. Patel, A.H. (2007). Industrial Microbiology, Macmillan India Limited, New Delhi.

2. Stanbury, P.F. and Whitaker, A., (Eds). (1984). Principles of Fermentation Technology,

Pergamon Press, Oxford.

3. Frazier, W.C. and Dennis C. Westhoff. (1995). Food Microbiology, Tata McGraw Hill

Publishing Company, New Delhi.

4. Casida, L.E. (2003). Industrial Microbiology, New Age International (P) Ltd., New Delhi.

5. Michael Shuler. and Fikret Kargi. (2002). Bioprocess Engineering: Basic Concepts, 2nd

Edition, Prentice Hall, Englewood Cliffs, NJ.





Credits : 5

Hours/week : 6


Semester : VI

CC11 - ENZYME TECHNOLOGY

UNIT-I

Enzymes - history and general characteristics, definition and IUB enzyme classification. Enzyme

specificity, holoenzyme, apoenzyme, coenzyme, cofactors, activators, inhibitors, active site,

metallo enzymes.

UNIT-II

Enzyme kinetics - effect of pH, temperature, activator, enzyme and substrate concentration -

Michaelis Menten plot and inhibitor kinetics (competitive, uncompetitive and non- competitive).

Lineweaver Burk plot, Eadie-Hofstee plot and Hanes Woolf equation. Significance of Km and

Vmax.

UNIT-III

Enzyme regulation - allosteric modification of enzymes, feedback inhibition and forward

simulation. Mode of enzyme action - lock and key hypothesis and induced fit hypothesis.

UNIT-IV

Enzyme catalysis - acid base catalysis, bond catalysis, strain, proximity and orientation effects.

Mechanism of action of lysozyme, chymotrypsin, enzyme substrate complex formation -

bisubstrate (random and ping pong mechanism).

UNIT-V

Isolation, extraction and purification of enzymes - enzyme immobilization -advantages and

disadvantages of immobilization techniques and its applications.


1. Palmer T. (2004). Enzymes: Biochemistry, Biotechnology and Clinical Chemistry, West Press

Edition.

2. Geoffrey L. Zubey., William. W. Parson. and Dennis E. Vance. (1995). Principles of

Biochemistry, W.M.C. Brown Publisher.

3. Stanbury, P.F., A. Whitaker. and S.J. Hall. (1997). Principles of Fermentation Technology,

Aditya Books Pvt. Ltd., India.





Credits : 4

Hours/week : 5


Semester : VI

CC12 - ENVIRONMENTAL BIOTECHNOLOGY

UNIT-I

Environment - basic concepts and issues. Environmental Pollution, Water Pollution, sources of

pollution and pollutants. Industrial effluents and Domestic wastes. Agrochemical - Heavy

metals. Effects, prevention and control of water pollution - Water pollution analysis and

monitoring. Soil pollution - sources, effects and its control.

UNIT-II

Air pollution - sources, air pollutants, effects and control measures. Ozone depletion, global

warming - Air pollution analysis and monitoring. Noise pollution, radioactive pollution, Thermal

pollution - their sources, effects, prevention and control measures.

UNIT-III

Biopesticides: Bacterial (Bt pesticides), fungal (Trichoderma); Viral biopesticides -Baculovirus,

NPV insecticides, Production of biofertilizers and biopesticides for large scale application.

UNIT-IV

Biofertilizers and their importance in crop productivity - Algal and fungal (mycorrhizae)

biofertilizers, bacterial biofertilizers (rhizobial, free living N2 fixers and phosphate solubilising

bacteria) and their significance and practice.

UNIT-V

Waste water treatment - Sewage and common Industrial effluent treatment - Concepts of

bioremediation (in-situ & ex-situ), Bioremediation of toxic metal ions - Biosorption and

Bioaccumulation principles. Concepts of Phyto-remediation - Microbial biotransformation of

pesticides and Xenobiotics, Microbial leaching of ores - direct and indirect mechanisms.


1. Mohapatra, P. K. (2006). Text Book of Environmental Biotechnology, I K International

Publishing House Pvt. Ltd., New Delhi.

2. Indu Shekhar Thakur. (2011). Environmental Biotechnology: Basic Concepts and

Applications, 2nd

Edition, I K International Publishing House Pvt. Ltd., New Delhi.

3. De, A.K. (2004). Environmental Chemistry, Wiley Eastern Ltd., New Delhi.





http://www.abebooks.com/servlet/SearchResults?an=P.K.+Mohapatra&cm_sp=det-_-bdp-_-author

Credits : 4

Hours/week : 3


Semester : VI

MAJOR PRACTICAL – IV

CC13 - LAB IN BIOPROCESS TECHNOLOGY, ENZYME TECHNOLOGY AND

ENVIRONMANTAL BIOTECHNOLOGY

Lab in Bioprocess Technology

1. Isolation of pure culture - E. coli, Streptomyces.

2. Isolation of industrially important organisms for the production of amylase and cellulose.

3. Growth - growth curve, measurement of bacterial population by Turbidometry and Serial

dilution methods.

4. Study of optimal culture conditions for the production of amylase in a bioreactor.

Lab in Enzyme Technology

1. Isolation, fractionation and purification of enzymes - ALP.

2. Effect of pH, temperature and enzyme substrate concentration on the activity of enzymes

ALP.

3. Gel filtration chromatography.

4. Ion Exchange Chromatography.

Lab in Environmental Biotechnology

1. Estimation of Nitrate in Drinking water.

2. Determination of COD and BOD in Sewage water.

3. Determination of Total Dissolved Solids and Suspended Solids of water.

Credits : 4

Hours/week : 4


Semester : VI

MEC3 - BIOTECHNOLOGY AND HUMAN HEALTH

UNIT-I Human Genetics and Human Genome - History and development of human genetics -

Organization of the human genome. Chromosome and gene organization - Inherited human

diseases - single gene diseases, complex traits.

UNIT-II Gene Therapy - Identification and isolation of disease genes - Cancer genetics - Genetic

counseling. Gene therapy. Infectious Diseases - Classification: protozoan, fungal, helminthic,

bacterial and viral. Vaccines - types. Hospital - acquired infections (nosocomial), water-borne

diseases.

UNIT-III Antigens and Antibodies - Acquired and Innate Immunity, Immune system, Immune diseases,

Allergy. Immunity to infections by viruses, bacteria, fungi and parasites. Blood groups.

Monoclonal antibodies.

UNIT-IV

Embryonic Stem Cells - Culture and Therapy. Artificial blood. Amniocentesis. Biochemical and

molecular diagnostics.

UNIT-V

IPR - Patents and Copyrights. Human Cloning - Prenatal sex determination and foeticide.

Genetically Modified Organisms.


1. Schacter, Bernice (Ed.). (2006). Biotechnology and Your Health : Pharmaceutical

Applications, Chelsea House Publications, New York.

2. Dinesh, K.P. and Chetan, D.M. (2007). Health and Pharmaceuticals Biotechnology, Laxmi

Publications (P) Ltd., India.

3. Crommalin, D.J.A., R.D. Sindeler. and B. Meibohm (Eds). (2007). Pharmaceuticals

Biotechnology: Fundamentals and Applications, Informa Health Care, London.





SYLLABUS FOR

NON MAJOR ELECTIVE

NMEC1 - RR5BTELO1 for B.Sc., (Statistics) students

NMEC2 - RR6BTELO2 for B.Sc., (Biochemistry) students

DEPARTMENT OF BIOTECHNOLOGY

Credits : 4

Hours/week : 4

Medium of Instruction : English Code: RR5BTELO1 / RR6BTELO2

Semester : V / VI

NMEC1 / NMEC2 - HEALTH EDUCATION

UNIT-I

Dimensions and Determinants of health, Indicators of health - Characteristics of indicators,

Types of indicators, Disease agents - Classification of disease agents.

UNIT-II

Nutrition - Classification and functions of food, sources and requirement of Carbohydrates,

Proteins, Fats, Vitamins and Minerals, Malnutrition - Protein energy Malnutrition (PEM),

Balanced diet - Composition of balanced diet

UNIT-III

Water - Safe and wholesome water, criteria for water quality standards, household purification

of water. Air - Health effects of air pollution, prevention and control Ventilation - Standards

of ventilation, Light - The requirements of good lighting,

UNIT-IV

Noise - Effects of noise exposure, Types of mental illness - Major and minor illnesses, Causes

of mental ill health - Social pathological causes, Preventive aspects - Primary - Secondary -

Tertiary.

UNIT-V

Immunization - Vaccines and Immunization Schedule, Principles of disease control and

prevention.


1. Murugesh, N. (2002). Health Education and Community Pharmacy, 3rd

Edition, Sathya

Publishers, Madurai.

2. Park, J.E. and Park. (2000). Text book of Preventive and Social Medicine, 17th

Edition,

Banarasidas Publishers, Jabalpur, India.

3. Khurana, S.P.S. (2007). Health Education and Community Pharmacy, S. Vikas Company,

India.

4. Kumar, N. (2010). Health Education and Community Pharmacy, 1st Edition, AITBS

Publishers, New Delhi.





Documents

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