Department of PhysiologyAnnual Report

July 1, 1969 to June 30, 1970

ACADEMIC STAFFE. B. Brown, Jr., Ph. D., Professor and ChairmanAlan M. Thompson. Ph. D., ProfessorDonald Johnson, Ph. D., ProfessorL. P. Sullivan, Ph. D., ProfessorG. N. Loofbourrow, Ph. D., Associate ProfessorJames Rhodes, M. D., Assistant ProfessorJohn Y. Trank, Ph. D., J:.ssistant ProfessorC. Merrill Tarr, Ph. D., A ssistant ProfessorR. L. Clancy, Fh. D., Assistant ProfessorWolkoff, A. S., M. D. t .Assistant ProfessorDennis Waring. Ph. D., Instructor, 12/1/69 to 5/30/70

Dr. Sullivan has been on leave since September 1969, working withDr. Gerhard Giebisch at Yale University. He expects to return tohis post in this department about August IS, 1970.

Dr. Goetz has accepted a position as Director of Research at St. Luke'sHospital, and his research program has been moved to that institution.He will continue his appointment in this department as Lecturer.

PCSTDOCTORAL FELLOWSAntonio Garcia (Carillo), M. D., 8/1/69-7/31/70Paul Hens1eigh, M. D., Supported by Ob-Gyn Training GrantArlo Hermreck, M. D.Eugene Luckstead. M. D., 7/1/69-6/30/70 (Pediatrics Dept. )Sharanjeet Singh, M. D., 1/1/69 to 11./31/69 (Pediatrics Dept. )David Stubbs, M. D., 10/1/69-6/30/70 (Pediatrics Dept. )

GRADUATE STUDENTS Degree Sought

* Paul Hens1eigh, M. D. Ph. D.Conrad Johanson II

Martin Kramen :,

Dennis Waring ItGary Bond IICarol Hendley i:

* Arlo Hermreck, M. D. II

Lester Wade "

Yih Loong Lai II

Chung-ning Pang It

Kyra Riegel "Pat Jurewicz "

## Frank Scamman "## Steve Bredehoeff: "## Richard Williams - M. A.

## Both Medical Student and Graduate Student

*Postdoctora1 and Graduate Student for Ph. D.

Date ExpectedFalll970" II

" 1969

Spring '70Fall J 70'1 '70" '70

Spring '11" '12" '72'I '72." '73

1970

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KAW VALLEY HEART STUDENTSDonna BenitzDona ShawMartin CramerRoger Tarbutton

(The Scienc~ Talent Search Program)

SUMMER RESEARCH FELLOWSDon BurkmanSteve Romondo

Ray RossRalph (Cullen) Thomas

(Medical Students)

TEACHING

The Physiology for Medical Students course 270-271 was again scheduledduring the winter and spring quarters with the last two weeks of the yeardevoted to the combined course in Endocrinology.

GRADUATE COURSES

Fall 1969 Course #310, Transport and Permeability - Dr. A. M. ThompsonII II Course #322 Physiology of the Nervous System, Dr. G. N. Loofbourrow

Spring 1970 Course #318 Gastroenterology -Dr. James Rhodes

GRADUATE SEMINAR COURSE #351A graduate seminar (1 semester credit) on "Physiological Clocks" was offeredby Dr. D. C. Johnson. The theme was on establishment, maintenance, andalteration of physiological rhythms. particularly those of the endocrine system.

Dec. 8. 1969

OUTSIDE SPEAKERS .AND VISITORS

Nov. 7. 1969

Jan. 19, 1970

Feb. G. 1970

Feb. 16. 1970

Dr. Wilford P. Stratten. Postdoctoral Fellow. Dept. Neurology,Univ. of Utah, IIDiazepam and Presynaptic Inhibition. 1\

Dr. Frank A. Brown, Jr., Morrison Professor of Biology,Northwestern Univ. "Organismic responsiveness to thegeoelectromagnetic fields. II

Jared Grantham, M. D.. Asst. Prof. Dept. of Medicine. KUl~C."Studies of Surface deformability in renal tubular cells. "

Lu Erwin. Ph. D., Postdoctoral Fellow. Dept. of Physiologyand Cell Biology. Univ. of Kansas at Lawrence. Kansas."Biochemical correlates of brain function. "

Fred Samson. Ph. D.. Professor, Dept. of Physiol. and CellBiology. Univ. Kansas. Lawrence, Kansas. "Transportof material along axons. il

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Cutside Speakers continued)

Mar. 2, 1970

March 9, 1970

March 16, 1970

March 26, 1970

:March 27, 1970

April 7, 1970

May 18, 1970

June 15, 1970

J. David Bristow, J ssoc. Prof., Dept. of Medicine,Univ. of Cregon Medical School, Portland, Oregon."Some observations on reflex control of heart rate in man. II

Dr. Robert Manning, J ssoc. Dean of Student Affairs, KUMC."Learning Systems Approach in Medical Education. II

Wm. M. Bass, Ph. D., Prof. Dept. of Physical Anthropology,Univ. of Kansas, Lawrence, Kansas. "Human Evolution. II

Dr. Francis J. Haddy, Chairman Dept. Physiology, MichiganState University, East Lansing, Michigan. "C sm01arityand Vascular Resistance. 11

Prof. Christian Crone, Head of Institute of Medical Physiology A,Univ. of Copenhagen, Denmark. "Studies on the clearance ofextracellular tracers from muscle tissue. It

Dr. C. D. Withrow, Pharmacology Dept., Univ. of UtahCollege of Medicine, Salt Lake City, Utah. "Ti"ssue HydrogenIon Regulation. "

Hans Haas, M. D., Ph. D., Wissenschaftlicher Rat,Physiologisches Institut der Universitat, Heidelberg, Germany.I'Kinetics of Fast Sodium Inward Current in Cardiac Muscle. il

Dennis R. Hafemann, Ph. D., Asst. Prof. of Physiol., Univ.California, Los)\, ngeles School of Medicine, Center forHealth Sciences, Los Angeles, California. "Binding ofIsotopically Labelled Tetrodotoxin to Nervous Tissue. II

TEACHING

The Physiology Course for Medical Students was again taught during theWinter and Spring quarters, with the last two weeks of the year devoted to thecombined course in Endocrinology. A considerable amount of time has beendevoted during the year to planning for the new curriculum. .A tentative outlineof the core course in physiology which will be taught in one six-weeks moduleplus a part of a second module has been compiled by the staff of this department.Before'this schedule was proposed a complete outline of cour se content for sucha course was compiled. Weare planning now to teach physiology as a block,rather than concomitantly with Biochemistry, during the coming academic yearin order to obtain some experience with this type of teaching.

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Graduatecourseswent on tothesemester system last year and thisrequired rearranging of our schedule. We now have two graduate coursesscheduled for each semester. rotating over a two-year basis. During thefall semester Dr. Thompson's course on Transport and Permeability. andDl. Loofbourrow's course on Physiology of the Nervous System were

offered. During the sprin~erm Dr. Rhodes' course on Gastroenterology

was offered. ,Since Dr. Su~iva!1 was on leave, his course on Renal ElectrolyteExcretion was not present~d. This year has been another very productive onefrom the standpoint of our graduate pro~ram. Since the last report MartinKramen, D~nnis Waring. Gary Bond' and Dr. Paul Hensleigh have completedwork for the Ph. D. degree. Three additional candidates. Conrad Johanson.Carol Hendley, and Dr. Arlo Hermreck are completing their thesis and willreceive their Ph. D. degrees in the next commencement.

At the invitation of Dr. Frank T. Y. Liu, A ssociate Professor and

Chairman of Physiology at the University of Missouri-Kansas City School ofDentistry. Kansas City, Missouri. Drs. Brown. Thompson. Trank. Clancy.Loofbourrow. and Johnson participated in teaching at the Dental College.

ACTIVITIES OF STAFF

Dr. E. B. Brown. Jr.. Ph. D. - Professor and Chairman

August 1969 - APS meetings at University of California. Davis.

Sept. Z6. Z7, 1969 - Kansas Heart Association Annual meeting and DelegateAssembly. Topeka. Kansas

Cct. 7. 1969 - Midwest Great Plains Region of AAMC (Council of Faculties)meeting in Chic,ago, Illinois.

Nov. 1, Z, 1969. Council of Academic Societies Eighth Annual Conference onResearch in Medical Education at Hilton Hotel in Cincinnati, Chio.

Jan. lZ, 13. 1970 - Midwest Great Plains Regional AAMC Council of Faculties.meeting in Chicago. Illinois.

Jan. 30. 1970 - Exec. Comm. of American Heart P ssn. Great Plains RegionResearch Review Committee. K. C.. Missouri.

Feb. 5. 1970 - Council of Academic Societies of AAMC - Chicago. Ill.

AprillZ. 1970 - Assoc. of Chairmen of Departments of Physiology meetingsin Atlantic City. N. Y.

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April 13-16, 1970,At 1ant ic City.

Attended American Physiological Society meetings in

May 20-23 - Third Annual Alfred Benzon Symposium, Copenhage.n and Lund.

Mar. 12-13 - American Heart Assn. .' Gl eat Plains Regional Research Reviewand Advisory Comm. meeting in Minneapolis, Minnesota (full committee).

April 3 - Kansas Heart Assn. Planning Council meeting, Vi ichita, Kansas.

April 28 - Kansas Heart .Association Program Coordinating Committee meetingin Topeka, Kansas.

May 9 - Kansas Heart Association Board of Directors meeting, Topeka, Kansas.

CFFI CES:

Chairman, Am. Heart Assn. Regional Research Review Committee.Chairman, Kansas Heart Assn. Research Committee.

Secretary-Treasurer, Assoc. Chairmen of Departments of Physiology.American Physiological Society's Representative to AAMC Council on

Education and Hospitals.KUMC Representative to Council of Faculties of Midwest Great PlainsRegional Assn. of AAMC.

LECTURES:

Nov. 18 - "Mechanics of Breating I for Am. Assn. for I nhalation Therapy,Nov. 22 - IIFundamentals of Acid-Base Balance!1 for St. Louis Society of

Anesthesiologists, St. Louis, Missouri.May 22 - I'The Buffer Capacity of the Whole Eody" at the Alfred Benzon

Symposium on Acid-Base and Potassium Homeostasis of the Brain,"Copenhagen, Denmark, and Lund, Sweden.

June 3 - tPotassium metabolism and Acid-Base Changes' at PhysiologyLaboratories, Cxford University, Oxford, England.

K. C., Mo.

Richard L. Clancy, Ph. D., Assistant Profes SOl'

Nov. 13-15, 1969 - j' ttended A nnual Meetings of the .American Heart A ssn.in Dallas, Texas.

April 1970 - Attended F ASEB meetings in .P tlantic City, New Jersey.

Sponsored paper F ASEB, Spiten, D. G., C. W. Smith, R. L. Clancy,'Effect ofAcid-Base changes on myocardial K+. '

Presented seminar Pediatric Cardiology Staff.

Myocardial °2 consumption.

I Deterrr.inants

Dr. David Stubbs. Fellow in Cardiology spent a year working in mylaboratory.

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1/20/70 - Mt. Marty College, Yankton So. Dakota group from chemistry dept.toured our facilities. Sister Kilzer, eSB, wrote letter thanking us for excellentlectures and demonstrations which made their visit worthwhile.

5/7/70 Sigma Xi lecture WHE Auditorium - KUMC Campus.of Starling's Law of the Heart. 1

" .P re-examination

Kenneth L. Goetz, Ph. D. ,M. D. - Lecturer

Attended Am. Heart Assn. Annual meetings in Dallas in Nov. 1969.

Finished medical internship at St. Luke's Hospital, K. C., Missouri.

Named Director of Experimental Medicine, St. Luke's Hospital, Jan. 1, 1970.

Attended Cardiology Conference in La Jolla, Cci.lifornia, Dec. 4-6, 1969.

Presented paper at F ASEB meetings in April 1970.

Continued series of physiology lectures to staff, St. Luke's Hospital.

Presented series of lectures on cardiovascular physiology to coronary carenursing class of Regional Medical Programs.

Served as consultant to Midwest Research Institute.

Gave seminar to Dept. of Biomedical Engr., Case Western ReserveUniversity, July 1969.

Lectured to Medical Staff, Menorah Hospital, K. C., Missouri.

Donald C. Johnson, Ph. D. , Professor

Meetings Attended:

April 1-5, 1970 - Soc. for Gynecologic Investigation, New Orleans.

June 22-24, 1970 - Endocrine Society, St. Louis, Mo.

Aug. 31-Sept. 3, 1970 - APS Meetings in Bloomington, Indiana.

Seminar course in IIPhysiological Rhythms" - Fall 1969.

Lectures on Endocrinology at University of Missouri School of Dentistry, K. C. ,Mo.

Post-Doctorate fellows: Dr. Rehan Naqvi - Left Sept. 1969.Dr. Paul Hensleigh(M. D.) finished his Ph. D. July 1970.

Pre-doctorate fellows: Carol Hendley - finishes Ph. D. Sept. 1970.Pang, Chung-ning -

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G. N. Loofbourrow, Associate Professor

Writing Neurophysiology Textbook.

Consultant, V A Hospital, K. C., Mis souri.

Department Librarian 1969-70.

Clinic Auditorium - Lecture to Pathobiology 73 class on "Physiological

Aspects of Aging. II

Talk to Nursing Education class on ';Physio10gica1, Aspects of Aging. "Visited U. S. Air Force School of Aerospace Medicine, Brooks Air

Force Base, San Antonio, to study methods of instruction.

James Rhodes, M. D., Assistant Professor

Graduate Cour se inG. 1. Physiology - Spring 1970.

Midwest Gut Club - Denver - Feb. 1970.

F ASEB - j tlanticCity, N. J. - April 1970.

Am. Gastroenterology Soc. - Boston - May 1970

Speaker at K State Annual Student Research Day, Manhattan, Kansas

in May 1970.

Elected to Faculty Steering Committee.

SAMA Teaching Award - 1969.

Lawrence P. Sullivan, Ph. D., Professor

Dr. Sullivan on leave since September 1969 working with Dr. Gerhard

Giebisch at Yale University.

C. Merrill Tarr, Ph. D., Assistant Professor

Aug. 1969 - APS Meet. in Davis, C:'!..lif.,- presented a paper at this meeting

on "Effects of Manganese and tetrodotoxin on two inward currents in

cardiac muscle. II '

April 1970 - APS meetings in Atlantic City, New Jersey.

Served on Ad Hoc Committee for the Development of Faculty Promotion Policy

and Guidelines.

Advisor to Eugene Luckstead, M. D., Fellow in Cardiology who did research

in my laboratory from July 1969-June 1970.

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In charge of Department Seminar 1969-70.

Representative of Physiology Department for Biological Sciences Lecture

Series Program.

Elected to membership in American Physiological Society and

Biophysical Society.

Alan M. Thompson - Professor

Jan. 14-16, in Washington, D. C. as a member of the panel evaluating

postdoctoral fellowship applications for the National Science Foundation.

Feb. 11-13, Participating in Stroke Workshop sponsored by the National Institute

of Neurological Diseases and Stroke in Hilton Isla.nd, South Carolina.

Mar. 16, gave seminar at Washburn University, Biology Department.

Working in my laboratory this summer of 1969 were Steve Bruner, 2nd year

medical student and Martin Kramer from Drake as a Kaw Valley Fellow.

Was Referee for papers in SCIENCE.

Became Assoc. Dean of Graduate School June I, 1970.

Graduate Advisor for Conrad Johanson and Lester Wade.

John W. Trank, A ssistant Professor

Consultant in Biomedical Engineering to Midwest Research Institute.

Served on Council for Human Response to Vibration.

Prtsented a series of lectures on medical electronics instrumentation to

E. E. 128 course at Lawrence, Kansa.s.

Supervised departmental shop maintenance and design function.

Graduate advisor to Gary Bond who completed requirements for Ph. D.

July 1970.

Attended 7th Annual Rocky Mountain Bioengineering Symposium May 4-6in Denver, Colorado.

Presented lectures in cardiovascular physiology and hemodynamics to UMKC

Dental School.

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RESEARCH GRANTS ACTIVE DURING THE YEAR

PHS HlROI HE 12231. Intracellular Compensation for Chronic PCC2 Changes.-02 year 10/1/69-9/30/70 - Dr. E. B. Brown, Jr.

Kaw Valley Heart Grant #9725, 1/1/69-12/31/69. Extended in order to expendfunds remaining as of 1/1/70. "Respiratory Control" Dr. E. B. Brown, Jr.

PHS Training Grant H5TIGM1l37 -05 year. 7/1/68 to 6/30/70. ProgramDirector. - Dr. E. B. Brown, Jr.

NSF HGB-4314-Dr. L. P. Sullivan. 2/1/67 to 5/31/68 (Extended on 8/31/69)"Factors controlling renal excretion of ammonium, hydrogen, andPotassium. "

#5-K3-GM, 11.898-04 from 7/1/69 to 6/30/70. Dr. L. P. Sullivan. CareerDevelopment Award. "Renal Tubular Potassium Transport. "

HIR01 AM 10227 USPHS - Dr. Sullivan. "Diuretic agents and ion transport in thetoad bladder." 1/1/70-12/31/70.

H64R1321 Kansas Heart Assn. (extended). "Coronary blood flow as 'related toright heart pressure." Dr. K. L. Goetz.

USPHS HHE 11560-02 6/1/69-5/31/70 (H1723) "A study of atrial receptors. "Dr. K. L. Goetz.

USPHE 13623 - 01. "A study of atrial receptors." 6/1/70-5/31/71. Dr. K. L. Go"''''~.,

USPHS NB 07059-02. 1/11/69- 03 yr. "Nonelectrolyte transport a1teratio!l bperfused brain." 1/1/70 to 12/31/70 (#1920) - Dr. A. M. Thomp~:-n.

#9722 Kaw Vd.11eyHeart - 7/1/69 to 6/30/70 "Cardiovascular Control inReptiles. II Dr. J. W. Trank

H9215 Kansas Heart Grant 7/1169 to 12/31/69. "Stimulus pattern and cardiovascularcontrol function. II Dr. J. W. Trank.

Kansas Heart Association - 7/1/69 to 6/30/70.cardiovascular response. - Dr. Trank

Pulsatile stimulation and

NASA Institutional Grant '7/1/69 to June 30, 1970. Human response to vibration.Dr. Trank.

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#1548 KUMC Research Committee - 10/1/69 to 2/28/70.

Dr. A. M. Thompson.

"Brain Perfusion. "

NIH #40l-HD-03097-0l, 3/1/68-2/28/69 now in second year. "Folliclestimulating hormone control in the male. II Dr. Donald C. Johnson.

NSF #GB 8067, 9/1/68-8/31/70.Dr. D. C. Johnson.

"Hypophyseal Gonadotropin Patterns. "

NSF Grant - 2 yrs. ) $19,500, "Gonadotropin patterns in male and female rats. "

PHS #1 401 HE 12426-01, 2/1/69-1/31/72.Dr. C. Merrill Tarr.

"Voltage clamp of cardiac muscle. "

#9214 Kansas Heart Assn. 7/1/69-6/30/70 (extended to 6/30/71) "Inotropicagents and Ca++ - current in cardiac muscle, II (no additional funds).

#5 ROI AM 12329-02 PHS 6/1/68 to 5/31/71 - 3 year period - "Intestinalpeptidases and peptides hydrolysis" (Arthritis and Metabolic Diseases).Dr. James B. Rhodes. (AM-12329-03 NIH)

PUBLICA TIONS:

Bristow, J. D., E. B. Brown, Jr., D. J. C. Cunningham, R. C. Goode, M. G.Howson, and P. Sleight. "The influence of ventilation, carbon dioxide andhypoxia on the baroreceptor reflex in man. J. Physiol. (In press)

ThaI, A., Brown, E. B., Jr., Hermreck, A., H. Bell.SHOCK. A PHYSIOLOGIC BASIS OF THERAPY. Yearbook, Chicago.(In press. )

Brown, E. B., Jr. "Whole Body Buffer Capacity!!, in Proceedings of the AlfredBenzon Symposium III on Acid-Base and Potassium Romeostasis of theBrain. Munksgaard. (In press. )

Brown, E. B., Jr. and B. 1'. Attebery. "In vivo and in vitro CO2 dissociationcurves. In Respiration and Circulation, F ASEB Handbook (In press).

McDougal, B. A. and L. P. Sullivan. Studies of the Effect of Bendroflumethiazideon Sodium Transport and Metabolism in the Toad Bladder. J. Pharmacol.and Exp. Therap. 172:203-210, 1970.

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Goetz, K. L., G. C. Bond, A. S. Herrr.reck, J. W. Trank, M. Stearns, andM. R. McGuire, Plasrr.a ADH levels following small reductions in rr.eanatrial transrr.ural pressure in conscious dogs. Fed. Proc. 26:798, 1970Abstract).

Goetz, K. L., A. S. Herrr.reck, G. L. Slick, and H. S. Starke. AtrialReceptors and Renal Function in Conscious Dogs. (In press - Arr.. J.Physiol. )

Goetz, K. L., G. C. Bond, A. S. Herrr.reck, J. W. Trank. Plasrr.a ADH levelsfollowing a decrease in rr.ean atrial transrr.ural pressure. (In press -Am. J. Physiol.)

Haas, H. G. and M. Tarr. Merr.brane currents in frog atria as affected bymetabolic inhibition. (Abstract) Pflugers Archiv. 307:R3l, 1969.

Tarr, M., H. G. Haas and J. Trank. Effects of Manganese and Tetrodotoxin ontwo inward currents in cardiac rr.usc1e. The Physiologist (Abstract)12:371, 1969.

Johnson, D. C. and R. H. Naqvi. A positive feedback action of androgen onpituitary follicle stirr.u1ating horrr.one: Induction of a cyclic phenorr.enon.Endocrinology 85:881-886, 1969.

Johnson, D. C. and R. H. Naqvi, A sirr.plified augrr.ented ovarian weightassay for follicie stimulating hormone. Proc. Soc. Exper. BioI. and Med.133:536-539, 1970.

Naqvi, R. H. and D. C. Johnson. The effect of progesterone on androgen or estro-gen induced increases in endogenous FSH in irr.mature ferr.ale rats.Endocrinology 87 :418-421, 1970.

Johnson, D. C. Temporal aspects of estrogen stimulated increases in plasrr.aand pituitary follicle stirr.ulating horrr.one. Acta Endocrinologica (in press).