Embed Size (px)
Citation preview
Opportunities for cooperation between ChinAquaNet and Ghent University
Ghent, August 31, 2007
UGENT AQUACULTURE R&D CENTRE
Em. Prof. Dr. Maurice PensaertEm. Prof. Dr. Maurice Pensaert
Faculty of Veterinary MedicineDepartment of Virology, Parasitology and Immunology
Faculty of Veterinary MedicineDepartment of Virology, Parasitology and Immunology
Presentation title – AuthorUGent Aquaculture R&D Centre
slide 2 of 14
Laboratory for VirologyWhite Spot Syndrome Virus group
Prof. Dr. HansNauwynck
MeezanurRahman
MathiasCorteel
JoãoLima
PhuocLe Hong
Em. Prof. Dr. Maurice Pensaert
Prof. Dr. Patrick Sorgeloos
Dr. CesarEscobedo
founders scientific support
Dr. VictoriaAlday-Sanz
PhD’s
Laboratory of Virology – Em. Prof. Dr. PensaertUGent Aquaculture R&D Consortium
Presentation title – AuthorUGent Aquaculture R&D Centre
slide 3 of 14
Research lines
A. Finalized Research
Laboratory of Virology – Em. Prof. Dr. PensaertUGent Aquaculture R&D Consortium
Presentation title – AuthorUGent Aquaculture R&D Centre
slide 4 of 14
intramuscular route oral route
- virus stock production
- in vivo titrations
- standardization of inoculation proceduresusing SPF
1. Development of reproducible White Spot Syndrome Virus inoculation procedures
Penaeus vannameishrimp
Laboratory of Virology – Em. Prof. Dr. PensaertUGent Aquaculture R&D Consortium
Presentation title – AuthorUGent Aquaculture R&D Centre
slide 5 of 14
standardized procedures for delivering known doses of virus= reproducible result of acute disease and mortality
1. Development of reproducible WSSV inoculation procedures
intramuscular
0
25
50
75
100
0 24 48 72Time (hours post inoculation)
96 120
Cum
ulat
ive
mor
talit
y (%
)
control
30 SID50
studies on control and pathogenesis
Laboratory of Virology – Em. Prof. Dr. PensaertUGent Aquaculture R&D Consortium
Presentation title – AuthorUGent Aquaculture R&D Centre
slide 6 of 14
2. Testing of anti-viral agents for the control of WSSV
Time
Cum
ulat
ive
mor
talit
y 27°C
33°C
Time
Cum
ulat
ive
mor
talit
y
Cidofovir
Control
Laboratory of Virology – Em. Prof. Dr. PensaertUGent Aquaculture R&D Consortium
Presentation title – AuthorUGent Aquaculture R&D Centre
slide 7 of 14
3. Comparison of virulence of WSSV strains
Time
Cum
ulat
ive
mor
talit
y
WSSV Thai
WSSV Viet Viet
Thaidifference in mortality rate
difference in numbers of infected cells
Laboratory of Virology – Em. Prof. Dr. PensaertUGent Aquaculture R&D Consortium
Presentation title – AuthorUGent Aquaculture R&D Centre
slide 8 of 14
Research lines
A. Ongoing Research
Laboratory of Virology – Em. Prof. Dr. PensaertUGent Aquaculture R&D Consortium
Presentation title – AuthorUGent Aquaculture R&D Centre
slide 9 of 14
1. Study of White Spot Syndrome Virus pathogenesis upon oral inoculation
standardized oral inoculation of 10000 SID50in SPF Litopenaeus vannamei shrimp time course study
of target organs
gillsstomach
systemic in hemolymph
via heart
hematopoietic tissue and other internal organs such
as cuticular epithelium etc.
12 hpi 24 hpiantennalgland
Laboratory of Virology – Em. Prof. Dr. PensaertUGent Aquaculture R&D Consortium
Presentation title – AuthorUGent Aquaculture R&D Centre
slide 10 of 14
12 hpi
stomach
12 hpi
12 hpi
gills
internal organs
24 hpi
usefull for diagnostic purposes
1. WSSV pathogenesis
Study of cellular sites of replication by immunohistochemistry
antennal gland
Laboratory of Virology – Em. Prof. Dr. PensaertUGent Aquaculture R&D Consortium
Presentation title – AuthorUGent Aquaculture R&D Centre
slide 11 of 14
Research lines
A. Recently started research topics
Laboratory of Virology – Em. Prof. Dr. PensaertUGent Aquaculture R&D Consortium
Presentation title – AuthorUGent Aquaculture R&D Centre
slide 12 of 14
1. Attempts to establish shrimp cell cultures and ex vivo explant systems
- in vitro titrations of WSSV- virus isolation
- study of the viral replication cycle- in vitro testing of anti-viral agents
2. Production of monoclonal antibodies directed against shrimp and viral antigens
- develop cellular markers - identify virus receptors- make diagnostic kits
Laboratory of Virology – Em. Prof. Dr. PensaertUGent Aquaculture R&D Consortium
Presentation title – AuthorUGent Aquaculture R&D Centre
slide 13 of 14
3. Dual infections of WSSV and Vibrio
(in cooperation with ARC)
- reproduce Vibrio disease in SPF shrimp- study the clinical effect of secondary bacterial infections
Laboratory of Virology – Em. Prof. Dr. PensaertUGent Aquaculture R&D Consortium
Presentation title – AuthorUGent Aquaculture R&D Centre
slide 14 of 14
site of the Lab of Virology:
http://www.vpi.ugent.be/
Thank youThank you
Laboratory of Virology – Em. Prof. Dr. PensaertUGent Aquaculture R&D Consortium
