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UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012) (Uttarakhand Act No. 11 of 2013) Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23 UTTARANCHALUNIVERSITY ArcadiaGrant,P.O.Chandanwari,Premnagar,Dehradun, Uttarakhand- 248007,INDIA Detailed Course Structure & Syllabus of Bachelor of Science (Hons.)- Biotechnology Applicable for Batch: 2020-23 Under Choice Based Credit System (CBCS) UTTARANCHAL UNIVERSITY

Detailed Course Structure & Syllabus

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Page 1: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

UTTARANCHALUNIVERSITY ArcadiaGrant,P.O.Chandanwari,Premnagar,Dehradun, Uttarakhand-

248007,INDIA

Detailed Course Structure & Syllabus

of

Bachelor of Science (Hons.)- Biotechnology

Applicable for Batch: 2020-23

Under Choice Based Credit System (CBCS)

UTTARANCHAL UNIVERSITY

Page 2: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

B.Sc. (Hons.) Biotechnology-3 Years (Effective from Academic Year 2020-2023 odd semester)

Under Choice Based Credit System (CBCS)

Page 3: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

SEMESTER- I

S.No

Code

Paper Title L-T-P

Credit

Theory Practical

Total End

sem.

Int. End

sem.

Int.

1 TBBT 101 Biochemistry &

Metabolism 4-0-0 4 60 40 - - 100

2 TBBT 102 Cell Biology 4-0-0 4 60 40 - - 100

3 TBBT 103 Generic Elective-1

(GE-1) 4-0-0 4 60 40 - - 100

4 TBES 104 Environmental

Science (AECC-1) 2-0-0 2 60 40 - - 100

5 *RMB 105

*RMM105

Remedial Biology/

Remedial Math 2-0-0 0 - 50 - - -

6 ADP 106 Academic activity 0-0-2 1 - - - 50 50

7 PBBT 101 Biochemistry &

Metabolism 0-0-4 2 - - 25 25 50

8 PBBT 102 Cell Biology 0-0-4 2 - - 25 25 50

9 PBBT 103 Generic Elective-1

(GE-1) 0-0-2 1 - - 25 25 50

Total for 1 Sem. 16-0-12 20 600

*This is non credit course but minimum qualifying marks 40 % i.e. 20 out of 50 is required.

Page 4: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

SEMESTER- II

S.No

Code

Paper Title L-T-P

Credit

Theory Practical

Total End

sem.

Int. End

sem.

Int.

1 TBBT 201 Animal Physiology 4-0-0 4 60 40 - - 100

2 TBBT 202 Plant Physiology 4-0-0 4 60 40 - - 100

3 TBBT 203 Generic Elective-2

(GE-2) 4-0-0 4 60 40 - - 100

4 TBEC 204

English

Communication

(AECC-1)

2-0-0 2 60 40 - - 100

5 ADM 205

Biostatistics &

Computational

Biology

2-1-0 3 60 40 - - 100

6 ADP 206 Seminar 0-0-2 1 - - - 50 50

7 PBBT 201 Animal Physiology 0-0-4 2 - - 25 25 50

8 PBBT 202 Plant Physiology 0-0-4 2 - - 25 25 50

9 PBBT 203 Generic Elective-2

(GE-2) 0-0-2 1 - - 25 25 50

Total 16-1-12 23 700

Page 5: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

SEMESTER- III

S.No

Code

Paper Title L-T-P

Credit

Theory Practical

Total End

sem.

Int. End

sem.

Int.

1 TBBT 301 Genetics 4-0-0 4 60 40 - - 100

2 TBBT 302 General

Microbiology 4-0-0 4 60 40 - - 100

3 TBBT 303 Chemistry -1 4-0-0 4 60 40 - - 100

4 TBBT 304 Generic Elective-3

(GE-3) 3-0-0 3 60 40 - - 100

5 TBBT 305 Skill Enhancement

Course (SEC-1) 3-0-0 3 60 40 - - 100

6 ADP 306 Activity/ SWAYAM

/NPTEL 0-0-4 2 - - - 50 50

7 PBBT 301 Genetics 0-0-4 2 - - 25 25 50

8 PBBT 302 General

Microbiology 0-0-4 2 - - 25 25 50

9 PBBT 303 Chemistry -1 0-0-2 1 - - 25 25 50

10 PBBT 304 Generic Elective-3

(GE-3)

0-0-2

1 - - - 25 25

11 PBBT 305 Skill Enhancement

Course (SEC-1) 0-0-2 1 - - - 25 25

Total 18-0-18 27 750

Page 6: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

SEMESTER- IV

S.No

Code

Paper Title L-T-P

Credit

Theory Practical

Total End

sem.

Int. End

sem.

Int.

1 TBBT 401 Molecular biology 4-0-0 4 60 40 - - 100

2 TBBT 402 Immunology 4-0-0 4 60 40 - - 100

3 TBBT 403 Chemistry-II 4-0-0 4 60 40 - - 100

4 TBBT 404 Generic Elective-4

(GE-4)

3-0-0 3 60 40 - - 100

5 TBBT 405 Skill Enhancement

Course – 2 ( SEC-2)

3-0-0 3 60 40 - - 100

6 ADP 406 Seminar Presentation 0-0-2 1 - - 50 50 100

7 ADP 407

*Personality

Development

Program

0-0-4 0 - 50 - - -

8 PBBT 401 Molecular biology 0-0-4 2 - - 25 25 50

9 PBBT 402 Immunology 0-0-2 2 - - 25 25 50

10 PBBT 403 Chemistry-II 0-0-2 1 - - 25 25 50

11 PBBT 404 Generic Elective-

4(GE-4) 0-0-2 1 - - - 25 25

12 PBBT 405 Skill Enhancement

Course – 2 ( SEC-2) 0-0-2 1 - - - 25 25

Total 20-0-16 26 800

*This is non credit course but minimum qualifying marks 40 % i.e. 20 out of 50 is required.

Page 7: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

SEMESTER- V

S.No

Code

Paper Title L-T-P

Credit

Theory Practical

Total End

sem.

Int. End

sem.

Int.

1 TBBT 501 Bio-process

Technology 4-0-0 4 60 40 - - 100

2 TBBT 502 Recombinant DNA

technology 4-0-0 4 60 40 - - 100

3 TBBT 503 Discipline Specific

Elective – 1( DSE-1) 4-0-0 4 60 40 - - 100

4 TBBT 504 Discipline Specific

Elective– 2 ( DSE-2) 4-0-0 4 60 40 - - 100

5 ADP 505 Presentation

(Industrial training) 0-0-2 1 - - - 100 100

6 ADP 506 Reasoning Skills 1-0-0 1 - 50 - - 50

7 PBBT 501 Bio-process

Technology 0-0-4 2 - - 25 25 50

8 PBBT 502 Recombinant DNA

technology 0-0-4 2 - - 25 25 50

9 PBBT 503 Discipline Specific

Elective – 1( DSE-1) 0-0-2 1 - - 25 25 50

10 PBBT 504

Discipline Specific

Elective – 2 ( DSE-

2)

0-0-2 1 - - 25 25

Total 17-0-14 24 750

Page 8: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

SEMESTER- VI

S.No

Code

Paper Title L-T-P

Credit

Theory Practical

Total End

sem.

Int. End

sem.

Int.

1 TBBT 601 Bio Analytical Tools

& Techniques 4-0-0 4 60 40 - - 100

2 TBBT 602 Genomics and

Proteomics 4-0-0 4 60 40 - - 100

3 TBBT 603 Discipline Specific

Elective – 3 4-0-0 4 60 40 - - 100

4 TBBT 604 Discipline Specific

Elective – 4 4-0-0 4 60 40 - - 100

5 ADP 605 Dissertation(Major)

& Presentation 0-0-8 4 - - 150 50 200

6 PBBT 601 Bio Analytical Tools

& Techniques 0-0-4 2 - - 25 25 50

7 PBBT 602 Genomics and

Proteomics 0-0-4 2 - - 25 25 50

8 PBBT 603 Discipline Specific

Elective – 3 0-0-2 1 - - 25 25 50

9 PBBT 604 Discipline Specific

Elective – 4 0-0-2 1 - - 25 25 50

Total 16-0-20 26 - - - - 800

Grand Total

(I to VI Sem) 146 4400

Page 9: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

PROGRAM EDUCATIONAL OBJECTIVES (PEOs),

PROGRAM OUTCOMES (POs)

of

BACHELOR OF SCIENCE- BIOTECHNOLOGY- 3 Years

Page 10: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

BACHELOR OF SCIENCE (BIOTECHNOLOGY)

Program Educational Outcomes (PEOs)

PEO 1. Acquire profound knowledge and practical expertise pertaining to domains of

biotechnology and identify synergetic interaction with lifescience disciplines.

PEO 2. Exhibit competency and skills to pursue higher studies, career in industries,

laboratories, with sound ethical values.

PEO 3. Apply biotechnological approach considering ethical issues to address challenges

related to environment, bio-energy, health, medicine and industries.

PEO 4. Recognize inherent entrepreneurship potential of biotechnology domains and

implement techniques with critical thinking ability to design /develop process or

product.

PEO 5. Inculcate life-long learning ability along with competent communication and

professional skills.

Page 11: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

PROGRAMME SPECIFIC OUTCOMES (PSOs)

PSO 1. Exhibit sound understanding of fundamentals of basic and applied field of

biotechnology and allied sciences and demonstrate technical skills pertaining to

widespread bio-analytical tools and techniques.

PSO 2. Demonstrate ability to conduct laboratory based experiments, analyze and

interpret data with application of computer and biostatistics tools and

simultaneously enhance their presentation, communication and writing skills

PSO 3. Identify the potential and applicability of biotechnological process, technique to

design/derive a solution to problems pertaining to environmental & biodiversity

conservation, health & medicine, animal and plant science, agriculture, society

and industry.

PSO 4. Exhibit competency to work individually and in team with life long learning

ability and simultaneously identify wide spread career domains in

pharmaceuticals, biotechnology related industries, entrepreneurship and startups.

Page 12: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

PROGRAM OUTCOMES (POs)

PO 1. Acquire fundamental knowledge pertaining to concepts, principle and applications of

basic and applied field of biotechnology.

PO 2. Identify interdisciplinary approach of science by comprehensive understanding and

analyzing concepts of microbiology, computation biology, biostatistics and chemical

science.

PO 3. Exhibit ability to conduct experiments, analyze and interpret data and implement critical

thinking ability for investigating problems in biotechnology and allied fields

PO 4. Identify potential of biotechnological techniques and innovations and their

implementation for sustainable development, entrepreneurship development alongwith

associated ethical issues.

PO 5. Demonstrate adequate technical skills pertaining to biotechnological tools and

innovations bio-analytical techniques, computational programmes, analytical methods

utilized in biotechnology.

PO 6. Strengthen writing skills, communication skills and personality development through

seminars, academic activities, report writing, etc.

PO 7. Inculcate lifelong learning ability and eligibility to pursue higher studies, simultaneously

identify career prospects and opportunities.

Page 13: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

COMPREHENSIVE TABLE

of

Bachelor of Science- Hons. Biotechnology

Page 14: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

S.

No.

Course

code

Name PO1 PO2 PO3 PO4 PO5 PO6 PO7 PSO1 PSO2 PSO4 PSO5

TBBT

101

Biochemistry &

Metabolism 2 1 1 1 1.5 - 2 2 1 1 1 TBBT

102 Cell Biology

1.5 1.00 2.3 2 2.3 - 2.5 2.6 2.5 2 1.5 TBBT

103

Generic Elective-

1 (GE-1) 2.0 2.0 2.3 3.0 2.3 2.0 2.5 1.6 2.0 1.5 2.0

TBES

104

Environmental

Science (AECC-1) 1 - - 2 - 1 2 1 - 3 1 *RM

B 105

*RM

M105

Remedial Biology/

Remedial Math

1.2 1.5 - - - - - 2 1 1 1

3 2 2 1 1 - 1.2 2 1 - 1.2

ADP

106

Academic

activity 1 1 1 - 2 1 1 1 1.5 1 1

PBBT

101

Biochemistry &

Metabolism 1 1 1.3 1 1 - 1 1.5 1 1 1 PBBT

102 Cell Biology

2.2 - 2 2 2 - 1.5 1.7 1.7 1.7 1.3 PBBT

103

Generic Elective

-1 (GE-1) 2.2 1.5 2.0 2.0 1.5 1.3 1.5 2.0 2.5 1.6 2.0 TBBT

201

Animal

Physiology 2.0 2.0 2.0 1.0 2.0 1.0 2.5 2.0 2.0 2.0 2.0 TBBT

202 Plant Physiology

2.2 2.0 2 1.7 2 - 2 2 1.5 1.7 2

TBBT

203

Generic Elective

-2 (GE-2) 1.5 2 1 1 2 - 2 3 1 2 1

TBEC

204

English

Communication

(AECC-1) - - - - - 3 - - - - -

ADM

205

Biostatistics &

Computational

Biology 3 2.7 2.5

1 2

- 2 2.2 2.2 2.3 1.7

ADP

206 Seminar

3 2.7 2.5

1 2

- 2 2.2 2.2 2.3 1.7 PBBT

201

Animal

Physiology 1.6 2.0 2.0 1.0 2.3 1.3 2.5 2.0 2.5 1.5 2.0 PBBT

202 Plant Physiology

2.5 - 2.2 1.2 1.5 - - 2 1.7 1.5 2

Page 15: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

PBBT

203

Generic Elective

-2 (GE-2) 2 1 2 1 2.5 - 2 2 2 1 1 TBBT

301 Genetics 2 2 1 1 2 - 2 3 1 2 1

TBBT

302

General

Microbiology 2 1 1 1 1.5 - 1 2 1 1 1

TBBT

303 Chemistry -1

- 1.7 2 - - - 1.5 2 1 - 1

TBBT

304

Generic

Elective-3 (GE-

3)

2.7 2 1 1.6 - - 1 1.7 1.5 1.2 1

TBBT

305

Skill

Enhancement

Course (SEC-1)

2 1.5 2.3 - - 1.5 - 2 1 2.3 -

Drug Designing 2.0 2.3 2.0 1.6 1.5 1.0 1.5 2.0 2.0 2.0 1.7

ADP

306

Activity/

SWAYAM

/NPTEL 1 1 - - 1 3 - 1 1 2 -

PBBT

301 Genetics

2 1 2.3 1 2.5 - 2 2 2 3 1

PBBT

302

General

Microbiology 1 - 1 1 1 - 1 1.5 1 1 1

PBBT

303 Chemistry -1

- 1.5 1 - - - 1 1.5 1.3 - 1

PBBT

304

Generic Elective

-3 (GE-3) 2 1 1 1 - - 1 1 1.5 1.2 1

PBBT

305

Skill

Enhancement

Course (SEC-1)

1 2 - - - - - 2.5 - 1.7 2

Drug Designing

Lab 1.6 2.0 1.5 1.5 2.0 1.5 1.0 2.3 2.0 1.6 1.5

TBBT

401

Molecular

biology 2.2 - 1.6 1.5 1.5 - 2 2.2 2 2.5 -

TBBT

402

Immunology

2 2 1.6 2 2 - - 1.6 2 1.5 1

TBBT

403

Chemistry-II

1.5 1.5 2 1 2 - 1.5 2 2 2.6 2

Page 16: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

TBBT

404

Generic Elective

-4 (GE-4) 1.7 1 2.7 2 1 1 1 2

TBBT

405

Skill

Enhancement

Course–2 (SEC2) 2.0 2.0 3.0 1.5 1.5 1.5 2.0 2.0 1.2 2.0 2.0

Basic of Forensic

Science 2.0 2.0 2.0 1.2 1.3 1.0 1.3 1.50 1.75 2.00 1.75 ADP

406

Seminar

Presentation - - - - 1 3 - - - - -

ADP

407

*Personality

Development

Program

- - - - - 2 - - 2.5 - -

PBBT

401

Molecular

biology 2.2 - 2 1.5 1.5 - - 2.5 2.2 2.2 - PBBT

402 Immunology

1.5 1.5 2 1 2 - 1.5 2 2 2.6 2 PBBT

403 Chemistry-II

- 1.2 1 - - - 1 1.25 1.33 - 1 PBBT

404

Generic Elective

-4(GE-4) 1.7 - 1.2 2.7 - - 1.2 1 1 1 1.3

PBBT

405

Skill Enhancement

Course–2 (SEC-2) 1.7 2.0 3.0 1.5 1.5 2.0 2.0 2.0 2.2 1.6 2.0

Basic of forensic

science lab 1.6 2.0 1.6 2.0 1.3 1.3 1.5 2.33 2.33 2.00 1.75 TBBT

501

Bio-process

Technology 3 1.6 1.5 1 1.2 - - 2.75 2.5 2 1

TBBT

502

Recombinant

DNA technology 2.3 2.0 1.0 3.0 2.0 1.0 2.0 1.6 1.6 1.5 2.0

TBBT

503

Discipline

Specific Elective

– 1( DSE-1) 3 1.2 2

- 3

- 2 3 2 - 1.5

IPR,

Entrepreneurship,

Bioethics &

Biosafety

2.8 2 1 - - - 1 1.8 1.5 1.3 1

Microbial

Physiology 3 1 1 - - 1 2 2 1 1 1

Biostatistics 3 2.7 1.5

1.5

1.6 1.7 2.2 1.3 1.6 TBBT

504

Discipline

Specific Elective 1 - - 2 - 1 2 1 - 3 1

Page 17: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

– 2 ( DSE-2)

Environmental

Biotechnology 3 1 2 2 2.5 1.5 2 2 1.67 1

Medical

Microbiology 1 1 - 1 1 1 3 1 1 1

Plant Anatomy

& Physiology 2 1 - 1 1 1 3 1 1 1

ADP

505

Presentation

(Industrial

training) 1 1 1.5 - 1.6 - - 1 1 2 - ADP

506 Reasoning Skills

- - - - - - 2 - - - - PBBT

501

Bio-process

Technology 2.2 1.5 3 1 1.2 - - 2.2 2 2 1

PBBT

502

Recombinant

DNA technology 2.2 1.0 1.5 1.5 1.6 2.0 1.5 2.0 2.5 1.5 2.0

PBBT

503

Discipline

Specific Elective

– 1( DSE-1) 2 - 2.5

- 2.5

- 1.3 2 2.75 - 1.5

IPR,

Entrepreneurship,

Bioethics &

Biosafety

2 1 1 - - - 1 1 1.5 1.2 1

Microbial

Physiology Lab 2 1.5 - 1.5 - 2 2 2 2 1 1

Biostatistics Lab 1 2.6 1.3 - - - 1.3 - 2.3 1.3 -

PBBT

504

Discipline

Specific Elective

– 2 ( DSE-2)

1.3 - 2.2 1.5 2 - - 2 2.2 2.7 -

Environmental

Biotechnology

Lab 2 2 1.5 2 2 1 2 2 1 1.33 2

Medical

Microbiology

Lab 1 1 - 1 1 - 1 3 1 1 1

Plant Anatomy

& Physiology 2 1 1 - 1 - 2 3 1 1 1

TBBT

601

Bio Analytical

Tools &

Techniques

3 1.6 1.5 1.2 1.5 - - 2.75 2.5 1.75 1

Page 18: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

TBBT

602

Genomics and

Proteomics 3 2 1 2 2.5 2 2 2 1.5 1

TBBT

603

Discipline

Specific Elective

– 3 2 1 3 1.8 - - 1 1.5 - 2.3 -

Animal

Diversity - I 2.0 2.0 2.0 1.0 2.0 1.0 2.5 2.00 2.00 2.00 2.00

Plant Diversity-I 2.2 2.0 2 1.7 2 - 2 2 1.5 1.75 2

TBBT

604

Discipline

Specific Elective

– 4

3 1.6 1.5 1 1.2 - - 2.7 2.5 2 1

Animal

Biotechnology 2.5 2.0 2.3 3.0 2.3 2.5 2.5 1.6 2.0 1.5 2.0

Animal

Diversity- II 2.00 2.00

2.0

0 1.00

2.0

0 1.00

2.5

0 2.00 2.00 2.00 2.00

Plant Diversity-II 2 1 3 1.8 - - 1 1.5 - 2.3 -

ADP

605

Dissertation

(Major) &

Presentation 1.5 2.5 3 - 3

1.5

2 2 2.5 3 2

PBBT

601

Bio Analytical

Tools &

Techniques

2.7 1.6 2.5 1 1.2 - - 1.75 2.5 2 1

PBBT

602

Genomics and

Proteomics 2 1 1.5 2 2 1 2 2 1 2 2

PBBT

603

Discipline

Specific Elective

– 3 2 1 2.3 1.4 2 - 1 1 2 1.7 1.7

Animal

Diversity - I Lab 1.6 2.0 2.0 1.0 2.3 1.3 2.0 2.0 2.5 1.5 2.0

Plant Diversity-I 2.5 - 2.2 1.2 1.5 - - 2 1.75 1.5 2

PBBT

604

Discipline

Specific Elective

– 4

1 1.5 3 1 1.5 - - 2.3 1.3 2 1

Animal

Biotechnology 2.0 1.5 1.0 3.0 2.0 1.0 2.0 1.6 2.0 1.5 2.0

Animal

Diversity- II 1.6 2.0 2.0 1.0 2.3 1.3 2.5 2.0 2.5 1.5 2.0

Plant Diversity-II 2 1 2.3 1.4 2 - 1 1 2 1.7 1.7

Page 19: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

SYLLABUS

of

B.Sc Hons. Biotechnology

Page 20: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

SEMESTER –I

Page 21: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc (H) Biotechnology Programme Code 05

Course Code TBBT-101 Credit 4

Year/Sem 1/1 L-T-P 4-0-0

Course Name Biochemistry and Metabolism

Objectives of the Course:

1. To provide exposure to the students on biochemical reactions inside the living system

2. To provide the basic knowledge on biomolecules.

3. To provide students with detail knowledge about bioenergetics of cell.

UNIT I (Total Topics- 10 and Hrs-10)

Amino acids & Proteins:Structure & Function. Structure and properties of Amino acids, Types of

proteins and their classification, Forces stabilizing protein structure and shape. Different Level of

structural organization of proteins, Protein Purification. Denaturation and renaturation of proteins.

Degradation of proteins, Oxidative, Non-Oxidative deamination and decarboxylation of amino

acids; Urea Cycle.

UNIT II (Total Topics -11 and Hrs-10)

Carbohydrates: Structure, Function and properties of Monosaccharides, Disaccharides and

Polysaccharides. Homo & Hetero Polysaccharides, Mucopolysaccharides. Glycolysis: Fate of

pyruvate under aerobic and anaerobic conditions. Pentose phosphate pathway, Gluconeogenesis,

Glycogenolysis and glycogen synthesis. TCA cycle, Electron Transport Chain, Oxidative

phosphorylation.

UNIT- III (Total Topics -9 and Hrs-10)

Lipids: Structure and functions –Classification, nomenclature and properties of fatty acids, essential

fatty acids with suitable example. ß-oxidation of fatty acids. Biosynthesis of fatty acids & its

regulation; Ketogenesis & its significance

UNIT-IV (Total Topics -8 and Hrs-10)

Nucleic acids: Structure and functions: Physical & chemical properties of Nucleic acids,

Nucleosides & Nucleotides, purines & pyrimidines. Different types of DNA. Biosynthesis (De

novo & Salvage pathways) & degradation of purine & pyrimidine nucleotides.

UNIT-V (Total Topics -9 and Hrs-8)

Enzymes: Nomenclature and classification of Enzymes, Holoenzyme, apoenzyme, Cofactors,

coenzyme, prosthetic groups. Concept of free energy, Entropy, Enthalpy, Redox Potential &G0’.

High energy phosphate compounds, Free energy of hydrolysis of ATP. Vitamins.

Course Outcomes (COs)

TBBT-101 CO 1Apply knowledge of biomolecules synthesis, metabolic pathways along with their

fundamental principles in the field of biochemistry and life science.

TBBT-101 CO 2 Acquire knowledge of cellular level metabolism, metabolic disorders and their

impact on health.

TBBT-101 CO 3 Understand the designing of recent biochemical techniques and their respective

applications.

TBBT-101 CO 4 Implement and analyze the experimental results by using statistical methods.

Page 22: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

References:

Books

1. Satyanarayan, U., Biochemistry, Elsevier Publication, 2017,5th Edition.

2. Verma S.K., A Textbook of Plant Physiology,Biochemistry and Biotechnology, S Chand

publication.

Mode of Evaluation

Internal (MM-40) External (MM-60)

Component Sessional -1 Sessional -2 Assignments End Semester Examination

Weightage 10 10 20 60

CO-PO Matrix Biochemistry & MetabolismTBBT-101

Course

Outcome PO1 PO2 PO3 PO4 PO5 PO6 PO7 PSO1 PSO2 PSO3 PSO4

TBBT-101

CO 1 2 1 - - - - 2 2 - - 1

TBBT-101

CO 2 - 1` 1 - - - - - - 1 -

TBBT-101

CO 3 - - 1 1 2 - - - 1 - -

TBBT-101

CO 4 - - 1 - 1 - - - - -

Average

CO

(TBBT-

101) 2 1 1 1 1.5 - 2 2 1 1 1

Page 23: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc (H) Biotechnology Programme Code 05

Course Code PBBT-101 Credit 2

Year/Sem 1/1 L-T-P 0-0-4

Course Name Biochemistry and Metabolism Lab

Objectives of the Course:

1. To study about the principle of biochemicalor metabolic reactions, enzymatic activities,

buffers etc.

2. To learn about the techniques for the biochemical estimation and qualitative test of

biomolecules.

3. To expertise in analysis of cellular activity and the effect of physiological factors on it.

4. To develop the practical skill sample handling, preparation of buffers and assays

PRACTICALS

1. To study activity of any enzyme under optimum conditions.

2. To study the effect of pH, temperature on the activity of salivary amylase enzyme.

3. Determination of - pH optima, temperature optima, K m value, V max value, Effect of

inhibitor (Inorganic phosphate) on the enzyme activity

4. Estimation of blood glucose by glucose oxidase method.

5. Principles of Colorimetry: (i) Verification of Beer's law, estimation of protein. (ii) To

study relation between absorbance and % transmission.

6. Preparation of buffers.

7. Separation of Amino acids by paper chromatography.

8. Qualitative tests for Carbohydrates, lipids and proteins

Course Outcomes (COs)

PBBT-101 CO1. Acquire technical skill related to various techniques and process utilized in

biochemical studies

PBBT-101 CO 2. Identify underlying principle and process of quantitative and qualitative

estimation data interpretation and its application in biological studies.’

PBBT-101 CO 3. Ability to carry out enzymatic studies to investigate factors affecting enzymatic

activity and design graphical representation.

PBBT-101 CO 4. Data analysis and perception of testing samples

References:

Books

Sawhney S. K & Singh Randhir,Introductory Practical Biochemistry, Narosa Publications

Mode of Evaluation

Internal (MM-25) External (MM-25)

Component Internal End Semester Examination

Weightage 25 25

Page 24: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

CO-PO Matrix Biochemistry and Metabolism Lab PBBT-101

Course Outcome PO1 PO2 PO3 PO4 PO5 PO6 PO7 PSO1 PSO2 PSO3 PSO4

PBBT-101 CO1. 1 - - 1 1 - 1 2 1 - -

PBBT-101 CO 2. 1 1 1 - - - - 1 - 1 -

PBBT-101 CO 3. - - 2 - 1 - - - - 1 -

PBBT-101 CO 4. - - 1 - - - - - - 1 1

Average CO

(PBBT-101) 1 1 1.3 1 1 - 1 1.5 1 1 1

Page 25: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc (H) Biotechnology Programme Code 05

Course Code TBBT-102 Credit 4

Year/Sem 1/1 L-T-P 4-0-0

Course Name Cell Biology

Objectives of the Course:

1. To provide students intimate knowledge about the function as well as structure of molecular

components within the cell or living world.

2. To develop a deeper understanding of how DNA is organized in the nucleus.

3. To understand the signal transduction and its pathway in the cell.

4. To provide students basic understanding about molecular basis of cancer.

UNITS I (Total Topics- 8 and Hrs-10)

Cell: Introduction and classification of organisms by cell structure, cytosol, compartmentalization

of eukaryotic cells, cell fractionation. Cell Membrane and Permeability: Chemical components of

biological membranes, organization and Fluid Mosaic Model, membrane as a dynamic entity, cell

recognition and membrane transport.

UNIT II (Total Topics -8 and Hrs-10)

Membrane Vacuolar system, cytoskeleton and cell motility: Structure and function of microtubules,

Microfilaments, Intermediate filaments. Endoplasmic reticulum: Structure, Function including role

in protein segregation. Golgi complex: Structure, biogenesis and functions including role in protein

secretion.

UNIT- III (Total Topics -9 and Hrs-10)

Lysosomes: Vacuoles and micro bodies: Structure and functions Ribosomes: Structures and

function including role in protein synthesis. Mitochondria: Structure and function, Genomes,

biogenesis. Chloroplasts: Structure and function, genomes, biogenesis Nucleus: Structure and

function, chromosomes and their structure.

UNIT-IV (Total Topics -8 and Hrs-10)

Extracellular Matrix: Composition, molecules that mediate cell adhesion, membrane receptors for

extra cellular matrix, macromolecules, regulation of receptor expression and function. Signal

transduction. Cancer: Carcinogenesis, agents promoting carcinogenesis, characteristics and

molecular basis of cancer.

Course Outcomes (COs)

TBBT-102 CO 1. Understand and apply the principles, tools and techniques of cell Biology which

prepares students for further higher education, basic research and employment.

TBBT-102 CO 2. Identify and understand the technical skills in the field of cell biology which will

enhance their knowledge for analysis and research.

TBBT-102 CO 3. Provide knowledge in the field of cell biology which covers various health and

environmental issues.

TBBT-102 CO 4. Identify ethical values related to cancer biology.

References:

Books

1. Carp G., Cell Biology, Willey-India.

2. Verma R.S., Cell Biology, Genetics, Molecular Biology, Evolution and Ecology, S Chand

Page 26: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

publication.

3. Powar C.B., Cell biology, Himalaya publication.

Mode of Evaluation

Internal (MM-40) External (MM-60)

Component Sessional -1 Sessional -2 Assignments End Semester Examination

Weightage 10 10 20 60

CO-PO Matrix Cell Biology TBBT-102

Course Outcome PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PSO

1

PSO

2

PSO

3

PSO

4

TBBT-102 CO1. 3 - 2 2 2 - 3 3 2 2 2

TBBT-102 CO2. 1 - 3 2 3 - 2 2 2 2 1

TBBT-102 CO3. 2 - 2 2 2 - - 3 1 2 -

TBBT-102 CO4. 1 1 - - - - - - - - -

Average CO (TBBT-

102) 1.5

1.0

0 2.3 2 2.3 - 2.5 2.6 2.5 2 1.5

Page 27: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc (H) Biotechnology Programme Code 05

Course Code PBBT-102 Credit 2

Year/Sem 1/1 L-T-P 0-0-4

Course Name Cell Biology Lab

Objectives of the Course:

1. To provide students deeper knowledge about the structure and function of different

Components within the prokaryotic and eukaryotic cell.

2. To develop a deeper understanding and demonstration of dialysis, plasmolysis and de-

Plasmolysis.

3. To understand the basic principle and working of microtome preparation for animal tissue.

4. To provide students basic understanding of cell division in onion root tip and insect gonads.

PRACTICALS

1. Study the effect of temperature and organic solvents on semi permeable membrane.

Demonstration of dialysis.

2. Study of plasmolysis and de-plasmolysis.

3. Cell fractionation and determination of enzyme activity in organelles using sprouted

Seed or any other suitable source.

4. Study of structure of any Prokaryotic and Eukaryotic cell.

5. Microtomy: Fixation, block making, section cutting, double staining of animal tissues

like liver, esophagus, stomach, pancreas, intestine, kidney, ovary, testes.

6. Cell division in onion root tip/ insect gonads.

7.Preparation of Nuclear, Mitochondrial & cytoplasmic fractions.

Course Outcomes (COs)

1. Understand the basic principle and techniques related to cell biology which will prepare

students for higher education and employment in this field.

2. Students will learn the laboratory skills in cell biology which will enhance their analytical skills

and research knowledge.

3. Utilize laboratory skills to enhance basic understanding of eukaryotic and prokaryotic cell

structure and function.

4. Acquaint the knowledge of cell biology for understanding various physiological processes

which occurs in a living cell.

References:

Books

1. Majumdar, R. and Sisodia R., Laboratory Manual of Cell Biology, PP publication, 2018.

Mode of Evaluation

Internal (MM-25) External (MM-25)

Component Internal End Semester Examination

Weightage 25 25

Page 28: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

CO-PO Matrix Cell Biology Lab PBBT-102

Course Outcome PO1 PO2 PO3 PO4 PO5 PO6 PO7 PSO1 PSO2 PSO3 PSO4

PBBT-102 CO1. 2 - 2 3 2 - 2 2 2 1 1

PBBT-102 CO2. 2 - 2 2 2 - - 1 2 2 2

PBBT-102 CO3. 2 - 3 2 2 - 1 2 2 2 1

PBBT-102 CO4. 3 - 1 1 2 - - 2 1 2 -

Average CO

(PBBT-102) 2.2 - 2 2 2 - 1.5 1.7 1.7 1.7 1.3

Page 29: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc (H) Biotechnology Programme Code 05

Course Code TBBT-103 Credit 4

Year/Sem 1/1 L-T-P 4-0-0

Course Name Biotechnology and human welfare

Objectives of the Course:

1. To provide fundamental aspects of Biotechnology with detailed applications in Industry,

agriculture and environment.

2. To impart Potential application of biotechnology and genetic engineering at commercial level to

enhance agricultural production, reduce environmental hazards, development of diagnostic

techniques, genomics, forensics and health.

3. To impart training on fundamentals of science using different biotechnological tools and

techniques.

4. To promote research and Innovation in biotechnological research for benefit for human being.

UNIT I (Total Topics- 7 and Hrs-8)

Industry: protein engineering; enzyme and polysaccharide synthesis, activity and secretion, alcohol

and antibiotic formation.

UNIT II (Total Topics -7 and Hrs-8)

Agriculture: N2 fixation: transfer of pest resistance genes to plants; interaction between plants and

microbes; qualitative improvement of livestock.

UNIT- III (Total Topics -8 and Hrs-8)

Environments: e.g. chlorinated and non-chlorinated organ pollutant degradation; degradation

of hydrocarbons and agricultural wastes, stress management, development of biodegradable

polymers such as PHB.

UNIT-IV (Total Topics -8 and Hrs-8)

Forensic science: e.g. solving violent crimes such as murder and rape; solving claims of paternity

and theft etc. using various methods of DNA finger printing.

UNIT-V (Total Topics -8 and Hrs-8)

Health: e.g. development of non-toxic therapeutic agents, recombinant live vaccines, gene therapy

diagnostics, monoclonal in E.coli, human genome project.

Course Outcomes (COs)

TBBT-103 CO1.Apply the basic and advanced tools and techniques of biotechnology for higher

studies, employment and advanced research in industrial and academic scale.

TBBT-103 CO2.Develop the laboratory skills in the field of biotechnology which will enhance

their knowledge for research analysis.

TBBT-103 CO3.Provide knowledge about wide applications of biotechnology with human welfare

which cover various health, agriculture and environmental issues.

TBBT-103 CO4.Identify ethical values related to gene therapy and gene cloning with an impact on

society.

References:

Books

1. Singh, B. D., Expanding horizons biotechnology, Kalyani publication.

Page 30: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Mode of Evaluation

Internal (MM-40) External (MM-60)

Component Sessional -1 Sessional -2 Assignments End Semester Examination

Weightage 10 10 20 60

CO-PO Matrix Biotechnology and human welfare TBBT-103

Course Outcome PO1 PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PSO

1

PSO

2

PSO

3

PSO

4

TBBT-103 CO1. 3 2 2 3 2 1 3 2 1 2 2

TBBT-103 CO2. 1 - 3 - 3 3 - 1 3 - -

TBBT-103 CO 3. 2 2 2 - 2 2 2 2 2 1 2

TBBT-103 CO4. 2 - - 3 - - - - - - -

Average CO

(TBBT-103) 2.0 2.0 2.3 3.0 2.3 2.0 2.5 1.6 2.0 1.5 2.0

Page 31: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc (H) Biotechnology Programme Code 05

Course Code PBBT-103 Credit 1

Year/Sem 1/1 L-T-P 0-0-2

Course Name Biotechnology and human welfare Lab

Objectives of the Course:

1. To impart fundamental experimental aspects of biotechnology with detailed applications

invarious industries.

2. To design and develop the research and innovation projects in biotechnological research.

3. Provide practical knowledge about wide applications of biotechnology with human welfare

which cover various health, agriculture and environmental issues

4. Develop the knowledge of different biosafety levels work cultures of biotechnological tools and

techniques.

PRACTICALS

1. List of Indian and International Biotechnology research Institutes

2. List of Indian and International Biotechnology Industries

3. List of Biotechnology based funding agency in India.

4. To perform ethanolicfermentation using Baker’s yeast.

5. Study of a plant part infected with a microbe.

6. To perform quantitative estimation of residual chlorine in water samples.

7. Isolation and analysis of DNA from minimal available biological samples.

8.Case studies on Bioethics (any two).

Course Outcomes (COs)

PBBT-103 CO1. Identify the prospects of basic and advanced experimental tools and techniques of

biotechnology for employment and advanced research in industrial and academic scale.

PBBT-103 CO2. Demonstrate the experimental techniques related to biotechnology which will

enhance theirknowledge for research analysis.

PBBT-103 CO3. Provide knowledge about wide applications of biotechnology with human

welfare whichcover various health, agriculture and environmental issues

PBBT-103 CO4. Identify ethical values related to gene therapy and gene cloning with an impact

on society.

References:

Books

1. R.IanFreshney. Culture of Animal Cells: A Manual of Basic Technique and Specialized

Applications, Sixth Edition. John Wiley & Sons publications

2. Lisa A. Seidman, Kraus Ellen MaryBrandnerDiana, Mowery Jeanette., Laboratory Manual for

Biotechnology and Laboratory Science The Basics., Pearson publications

Mode of Evaluation

Internal (MM-25) External (MM-25)

Component Internal End Semester Examination

Weightage 25 25

Page 32: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

CO-PO Matrix Biotechnology and human welfare Lab PBBT-103

Course Outcome PO1 PO2 PO3 PO4 PO5 PO6 PO7 PSO1 PSO2 PSO3 PSO4

PBBT-103 CO1. 3 2 2 2 2 2 2 3 2 2 2

PBBT-103 CO2. 2 1 2 - 2 1 1 2 3 - -

PBBT-103 CO3. 2 2 - 1 1 1 - 2 - 2 2

PBBT-103 CO4. 2 1 - 3 1 - - 1 - 1 -

Average CO

(PBBT-103) 2.2 1.5 2.0 2.0 1.5 1.3 1.5 2.0 2.5 1.6 2.0

Page 33: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc (H) Biotechnology Programme Code 05

Course Code TBES104 Credit 2

Year/Sem 1/1 L-T-P 2-0-0

Course Name Environmental Science(AECC-1)

Objectives of the Course:

1. To acquire master foundational knowledge enabling them to have life–long learning related to

one’s surroundings.

2. To develop critical thinking skills in relation to environmental affairs and articulate

multidisciplinary context of the subject.

3. To acquire knowledge about natural resources and assess aesthetic and ethical importance of all

the living flora and fauna.

4. To interpret and propose solutions for effective management of different types of environmental

pollution

UNIT I (Total Topics- 10 and Hrs-6)

Introduction to Environmental Studies&Natural Resources: Multidisciplinary nature of

environmental studies; Land resources: Land degradation, Soil erosion and Desertification. Forest

Resources, Deforestation, Dams- benefits and problems; Mineral Resources- harmful effects of

mining.Water Resources- conflicts over water- Middle East Crisis & Indus Water Treaty.

UNIT II (Total Topics -9 and Hrs-6)

Ecosystem &Biodiversity: Ecosystem- definition, Energy flow in an Ecosystem: Food chains,

Food webs Ecological Pyramids. Biodiversity: Values of Biodiversity. Biogeographic zones of

India; Hot spots of India, Threats to biodiversity: Habitat loss, poaching of wildlife, man‐wildlife

conflicts.

UNIT- III (Total Topics -8 and Hrs-6)

Environmental Pollution: Air Pollution: causes, effects and control measures, case studies-

Donora Air Pollution Episode, Bhopal Gas Tragedy; Water Pollution: Sources, effects and control

measures.Noise Pollution: causes effects, control measures and Limits of noise as prescribed by

CPCB.

UNIT-IV (Total Topics -9 and Hrs-6)

Environmental Issues: Climate change, Global warming, Ozone layer depletion, Acid rain; Solid

waste management: Types of waste and management of waste. Environment and Public health.

UNIT-IV (Total Topics -6 and Hrs-6)

Environmental Ethics, Policies & Practices: Environmental Ethics, Environmental movements:

Chipko Movement and NarmadaBachaoAndolan; Environment Laws: Environment Protection Act;

Wildlife Protection Act; Forest Conservation Act. International agreements: Montreal and Kyoto

protocols.

Course Outcomes (COs)

TBES-104 CO1. Master foundational knowledge enabling them to have life–long learning related

to one’s surroundings.

TBES-104 CO2. Develop critical thinking skills in relation to environmental affairs and articulate

multidisciplinary context of the subject.

TBES-104 CO3. Acquire knowledge about natural resources and assess aesthetic and ethical

Page 34: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

importance of all the living flora and fauna.

TBES-104 CO4. Interpret and propose solutions for effective management of different types of

environmental pollution

TBES-104 CO5. Keep updated and communicate knowledge regarding social issues and laws

related to environment.

References:

Books

1. KaushikAnubha.,Environmental Studies

2. BaruchaErach.,Environmental Studies

3. Deswal&Deswal ,Environmental Studies

Mode of Evaluation

Internal (MM-40) External (MM-60)

Component Sessional -1 Sessional -2 Assignments End Semester Examination

Weightage 10 10 20 60

CO-PO Matrix Environmental Science(AECC-1)TBES-104

Course Outcome PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7 PSO1 PSO2 PSO3 PSO4

TBES-104 CO1. 1 - - 2 - 1 2 1 -- 3 1

TBES-104 CO2. 1 - - 2 - 1 2 1 - 3 1

TBES-104 CO3. 1 - - 2 - 1 2 1 - 3 1

TBES-104 CO4. 1 - - 2 - 1 2 1 - 3 1

TBES-104 CO5. 1 - - 2 - 1 2 1 - 3 1

Average CO

(TBES-104) 1 - - 2 - 1 2 1 - 3 1

Page 35: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc (H) Biotechnology Programme Code 05

Course Code RMB-105 (I) Credit 0

Year/Sem 1/1 L-T-P 2-0-0

Course Name Remedial Biology

Objectives of the Course:

1. To appraise the structure and function of cell and its division.

2. To acknowledge the different micro-organisms and their characteristics.

3. To inculcate the concept of genetics and heredity.

4. To understand the basics of Plant Biotechnology and characteristics of characteristics of

archegoniate, Bryophytes, Pteridophytes and Gymnosperms.

5. To interpret the immunological response and basic knowledge of immunity.

UNIT I (Total Topics- 7and Hrs-8)

CELL: Introduction To cell, types of cell (Eukaryotes, Prokaryotes) their general characteristics with

examples. Ultra microscopic structures of animal & plant cell. Cell organelles, their structure and

functions. Types of cell division. Mitosis and Meiosis (brief introduction)

UNIT II (Total Topics -6 and Hrs-8)

Micro-organism :-Introduction to Micro-organism, General Characteristics, Examples of each group for the following micro-organisms 1. Bacteria 2. Algae 3. Fungi 4. Virus

UNIT- III (Total Topics -6 and Hrs-8)

Genetics and Heredity :-Mendel’s laws- Monohybrid and Dihybrid cross, Structure of DNA and

types of RNA, mutation. The flow of genetic information from DNA to RNA to protein.

UNIT-IV (Total Topics -11 and Hrs-9)

Introduction to Plant and Biotechnology-Plant Diversity, brief study of Algae, fungi, bryophytes, pteridophytes, gymnosperms and angiosperms. Brief about root, stem, leaf, flower and seed. Introduction to Biotechnology-Plant biotech, food biotech, animal biotech, environmental biotech and

Genetic Engineering. Gene Cloning-Introduction and methodology in brief. Applications of Genetic

Engineering in plants- Insects and virus resistant plants

UNIT-IV (Total Topics -3 and Hrs-6)

Immunity-Introduction to Immune system and types of immunity. Brief about antigen and antibody

Course Outcomes (COs)

RMB-105 (I) CO 1.Understand the basic structure and function of cells.

RMB-105(I) CO 2.Appraise about different types of microorganisms, their shape, size, structure,

reproduction and importance

RMB-105(I) CO 3. Understand different modes of sexual reproduction in microorganisms.

RMB-105(I) CO 4.Interpret generation of immune response in human body

RMB-105(I) CO 5.Identify characteristics of archegoniate, Bryophytes, Pteridophytes and

Gymnosperms. References:

Books

1. Text books of NCERT for Biology

Page 36: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Mode of Evaluation

Internal (MM-50) External (MM)

Component Sessional -1 Sessional -2 Assignments End Semester Examination

Weightage 10 10 30 -

CO-PO Matrix Remedial Biology RMB-105 (I)

Course Outcome PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PSO

1

PSO

2

PSO

3 PSO4

RMB-105(I) CO 1. 2 - - - - - - 2 - - -

RMB-105(I) CO 2. - 1 - - - - - - 1 1 -

RMB-105 (I) CO 3. 1 - - - - - - 2 - - 1

RMB-105 (I) CO 4. 1 2 - - - - - 2 - 1 -

RMB-105 (I) CO 5. 1 - - - - - - - - - -

Average CO

(RMB-105 (I) 1.2 1.5 - - - - - 2 1 1 1

Page 37: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc (H) Biotechnology Programme Code 05

Course Code RMM-105 (II) Credit 0

Year/Sem 1/1 L-T-P 2-0-0

Course Name Remedial Mathematics

Objectives of the Course:

1. To develop the concepts of basics of matrix theory in multidisciplinary environment.

2. To identify and obtain the solution of the problems associated with binomial theorem.

3. To extend the concept of numbers from real number to complex number and formulate the

properties of complex numbers by using appropriate techniques.

4. To drive the various forms of straight line, circle, parabola and ellipse and its application in

multidisciplinary environment.

UNIT I (Total Topics- 10 and Hrs-6)

Elementary idea of complex number. Arithmetic and geometric progressions. Elementary idea of

permutation and combinations. Binomial theorem for positive integral index, any index and their

applications

UNIT II (Total Topics -9 and Hrs-6)

Co-ordinates: distance between two points, co-ordinates of a point of division of straight line joining

two points, equation of straight line: slope-intercept form, point slope form, two-points form, intercept

form, normal form, area of triangle and quadrilateral, equation of a circle, area of circle

UNIT- III (Total Topics -8 and Hrs-6)

Review of ratios of some standard angles, T-ratios of allied angles, Sum and difference formula and

their application. Inverse trigonometric functions. Introduction to matrices and determinants, addition,

subtraction and multiplication of matrices.

UNIT-IV (Total Topics -9 and Hrs-6)

Functions: Limit, continuity, differentiation, equations of tangent and normal, maxima and minima.

Elementary idea of probability theory.

UNIT-IV (Total Topics -6 and Hrs-6)

Methods of integration including integration by parts. Define integral, application of definite integrals

in finding areas under curves.

Course Outcomes (CO)

RMM-105(II) CO 1.Enhance the basics of matrix theory in different problems of mathematics and

science which is related to real world situation.

RMM-105(II) CO 2.Learn to fit series likes arithmetic and geometric progression to enhance critical

thinking ability.

RMM-105(II) CO 3. Developed the idea of expand the power of sum of two variable using binomial

theorem.

RMM-105(II) CO 4.Application of concept of numbers from real number to complex numbers by

using appropriate techniques.

References:

Books

1. Text books of NCERT for Mathematics

Page 38: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Mode of Evaluation

Internal (MM-50) External (MM-)

Component Sessional -1 Sessional -2 Assignments End Semester Examination

Weightage 10 10 30 -

CO-PO Matrix Remedial MathematicsRMM-105 (II)

Course Outcome PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7 PSO1 PSO2 PSO3 PSO4

RMM-105 (II) CO 1. 3 2 2 1 1 - 2 2 1 - 2

RMM-105 (II) CO 2. 3 - 2 1 1 - 1 2 1 - 1

RMM-105 (II) CO 3. 3 - 2 - 1 - 1 2 - - 1

RMM-105(II) CO 4. 3 2 2 1 1 - 1 2 - - 1

Average CO

(RMM-105 (II) ) 3 2 2 1 1 - 1.2 2 1 - 1.2

Page 39: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc. (H) Biotechnology Programme Code 500

Course Code ADP-106 Credit 1

Year/Sem 1/1 L-T-P 0-0-2

Course Name Academic activity

Objectives of the Course:

1. To demonstrate technical skills for effective preparation of presentations, write-ups through

participant in academic and extracurricular activities.

2. To exhibit good communication and presentation skills.

3. To acquire critical thinking ability to analyze and interpret observations, recent scientific

developments, etc

Each student has to participate in any one of the following mentioned academic activity. A

power point presentation will be presented by each student pertaining to the activity in which the

student has participated. A hard copy of the presentation will be submitted in the department.

Evaluation will be done based upon the presentation and report submitted.

Activities:

• Participation in seminar / conference / workshop

• Poster presentation / oral presentation in any other academic event (beside

seminar / conference) organized by departmental clubs / College / University /

research institute.

• Report of educational visit to industries, research institutes, etc

Course Outcomes (COs):

ADP-106 CO 1. Demonstrate technical skills for effective preparation of presentations, write-

ups through participant in academic and extracurricular activities.

ADP-106 CO 2 Exhibit good communication and presentation skills.

ADP-106 CO 3 Acquire critical thinking ability to analyze and interpret observations, recent

scientific developments, etc

Mode of Evaluation

Internal (MM-50) External

Component Internal Assessment End Semester Examination

Weightage 50 -

CO PO Matrix Academic activity ADP-106

Course

Outcome

PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7 PSO1 PSO2 PSO3 PSO4

ADP-106 CO 1. 1 - - - - - 1 1 2 - -

ADP-106 CO 2. - - - - 2 - - - 1 - -

ADP-106 CO 3. - 1 1 - - 1 - - - 1 1

Average CO

(ADP-106) 1 1 1 - 2 1 1 1 1.5 1 1

Page 40: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

SEMESTER-II

Page 41: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc. (H) Biotechnology Programme Code 500

Course Code TBBT-201 Credit 4

Year/Sem 1/2 L-T-P 4-0-0

Course Name Animal Physiology

Objectives of the Course:

1. To learn the applied knowledge of animal physiology metabolic pathways along with their

fundamental principles in mammalian body at cellular level.

2. To make students learn the application of physiology in the field of modern biomedical science.

3. To familiarize the students through scientific enquiry into the nature of mechanical, physical, and

biochemical functions of mammalian system.

4. To provides theoretical and practical designing of recent physiological techniques and their

conducting experiments in laboratories.

UNIT I (Total Topics- 10 and Hrs-10)

Digestion and Respiration: Digestion: Mechanism of digestion & absorption of carbohydrates,

Proteins, Lipids and nucleic acids. Composition of bile, Saliva, Pancreatic, gastric and intestinal juice.

Respiration: Exchange of gases, Transport of O2 and CO2, Oxygen dissociation curve, Chloride shift.

UNIT II (Total Topics -8 and Hrs-10)

Circulation: Composition of blood, Plasma proteins & their role, blood cells, Haemopoisis,

Mechanism of coagulation of blood. Mechanism of working of heart: Cardiac output, cardiac cycle,

Origin & conduction of heart beat.

UNIT- III (Total Topics -9 and Hrs-10)

Muscle physiology and osmoregulation: Structure of cardiac, smooth & skeletal muscle, threshold

stimulus, All or None rule, single muscle twitch, muscle tone, isotonic and isometric contraction,

Physical, chemical & electrical events of mechanism of muscle contraction.

UNIT-IV (Total Topics -12 and Hrs-10)

Nervous and endocrine coordination: Mechanism of generation & propagation of nerve impulse,

structure of synapse, synaptic conduction, saltatory conduction, Neurotransmitters. Mechanism of

action of hormones (insulin and steroids). Different endocrine glands– Hypothalamus, pituitary,

pineal, thymus, thyroid, parathyroid and adrenals, hypo & hyper-secretions.

Course Outcomes (COs)

TBBT-201CO 1.Applied knowledge of biomolecules, metabolic pathways along with their

fundamental principles in the mammalian body at the cellular level and system level.

TBBT-201CO 2.Understand the designing of recent physiological techniques with related to human

health system.

TBBT-201CO 3.Enhanced knowledge and appreciation of mammalian physiology and understand

the functions of important physiological systems including the cardio, respiratory, renal, digestive and

endocrine systems.

TBBT-201CO 4.Practicing group learning through scientific inquiry into the nature of mechanical,

physical, and biochemical functions of different systems of animal.

References:

Books

1. Subrahmaniyam S. and MadhavankuttyK., Text book of human physiology, S Chand publication.

Page 42: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

2. Bhaskar, H.V., Animal physiology, Campus Books International.

Mode of Evaluation

Internal (MM-40) External (MM-60)

Component Sessional -1 Sessional -2 Assignments End Semester Examination

Weightage 10 10 20 60

CO-PO Matrix Animal Physiology TBBT-201

Course Outcome PO1 PO2 PO3 PO4 PO5 PO6 PO7 PSO1 PSO2 PSO3 PSO4

TBBT-201CO 1. 2 2 - 1 - 1 3 2 - 2 2

TBBT-201CO 2. - - 2 - 2 1 - - 2 - -

TBBT-201CO 3. 2 2 - - - - 2 2 - 2 -

TBBT-201CO 4. - 2 2 - 2 1 - - 2 - 2

Average CO

(TBBT-201) 2.0 2.0 2.0 1.0 2.0 1.0 2.5 2.0 2.0 2.0 2.0

Page 43: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc (H) Biotechnology Programme Code 05

Course Code PBBT-201 Credit 2

Year/Sem 1/2 L-T-P 0-0-4

Course Name Animal Physiology Lab

Objectives of the Course:

1. To enable the students to acquire physiology based experiments of modern biomedical science.

2. To familiarize the students through scientific enquiry into the nature of mechanical, physical,and

biochemical functions of mammalian system.

3. To impart practical knowledge of fundamental principles in mammalian physiology.

4. To develop the theoretical based practical designing of recent physiological techniques and their

conducting experiments in laboratories.

PRACTICALS

1. Finding the coagulation time of blood

2. Determination of blood groups

3. Counting of mammalian RBCs

4. Determination of TLC

5. Determination of DLC

6. Determination of Haemoglobin

7. Determination of ESR.

Course Outcomes (COs)

PBBT-201CO 1.Demonstrate the experimental techniques related to mammalian body at the cellular

level.

PBBT-201CO 2. Understand the designing of recent physiological techniques and their conducting

experiments in laboratories.

PBBT-201CO 3.Generate and interpret the test hypotheses, analyze the data by using modern

methods.

PBBT-201CO 4.Practicing group learning through scientific inquiry into the nature of mechanical,

physical, and biochemical functions of different systems of animal.

References:

Books

1. Barbara A., Shirley Laboratory Manual of Mammalian Physiology 2nd Edition, Macmillan Pub

Co

Mode of Evaluation

Internal (MM-25) External (MM-25)

Component Internal End Semester Examination

Weightage 25 25

Page 44: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

CO-PO Matrix Animal Physiology LabPBBT-201

Course Outcome PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PSO

1

PSO

2

PSO

3

PSO

4

PBBT-201CO 1. 2 2 - 1 3 1 3 2 3 2 2

PBBT-201CO 2. 1 - 2 - 2 1 - 2 2 - -

PBBT-201CO 3. 2 2 - - - - 2 2 - 1 -

PBBT-201CO 4. - - 2 - 2 2 - - - - 2

Average CO (PBBT-

201) 1.6 2.0 2.0 1.0 2.3 1.3 2.5 2.0 2.5 1.5 2.0

Page 45: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc (H) Biotechnology Programme Code 05

Course Code TBBT-202 Credit 4

Year/Sem 1/2 L-T-P 4-0-0

Course Name Plant Physiology

Objectives of the Course:

1. To provide fundamental aspects of plant physiology with especial focus on plant water

relationship and mineral nutrition.

2. To learn basic concept of aerobic, anaerobic respiration and significance of photosynthesis.

3. To understand the basics of nitrogen metabolism studies and assesses dormancy and seed

germination in plants.

4. To learn about the growth, development and movements in plants.

UNIT I (Total Topics- 10 and Hrs-10)

Plant and water relationship, colligative properties of water. Water uptake, conduction,

transpiration, mechanism and its regulation by environmental variables. Mineral nutrition: Macro,

and micronutrients, their role, deficiency and toxicity symptoms, plant culture practices,

mechanism of ion uptake and translocation.

UNIT II (Total Topics -10 and Hrs-10)

Photosynthesis- Photosynthesis pigments with their structures, anoxygenic and oxygenic

photosynthesis, concept of two photo systems, photosynthetic pigments photophosphorylation,

physiology of bacterial photosynthesis: concept of light reactions, cyclic and non-cyclic

photophosphorylation. Carbon dioxide fixation, calvin cycle, C3, C4, CAM plants,

photorespiration, compensation point.

UNIT- III (Total Topics -7 and Hrs-10)

Nitrogen metabolism- inorganic & molecular nitrogen fixation, nitrate reduction and ammonium

assimilation in plants. Biofertilizers and biopesticides.

UNIT-IV (Total Topics -8 and Hrs-10)

Growth and development: Definitions, phases of growth, growth curve, growth hormones (auxins,

gibberlins, cytokinins, abscisic acid, and ethylene).Physiological role and mode of action, seed

dormancy and seed germination, concept of photo-periodism and vernalization. Physiology of

Senescence and Ageing.

Course Outcomes (COs)

TBBT-202 CO 1.Impart an insight into the various plant water relations and mineral nutrition in

plants.

TBBT-202 CO 2.Depict ability to identify and estimate various photosynthetic pigments found in

leaf and its application in botanical studies.

TBBT-202 CO 3 .Understand the mechanism of various metabolic processes in plants and access

role of seed germination and dormancy.

TBBT-202 CO 4.Equip students with skills and techniques related to plant physiology so that they

can design their own experiments, gain knowledge and make good career in this field.

References:

Books

1. Jain V.K., Fundamental of plant physiology, S Chand Publication.

Page 46: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

2. Verma S.K., A Textbook of Plant Physiology, Biochemistry and Biotechnology, S Chand

Publication.

3. Choyal R., Plant Anatomy and Physiology, Sonali Publication, 2012.

Mode of Evaluation

Internal (MM-40) External (MM-60)

Component Sessional -1 Sessional -2 Assignments End Semester Examination

Weightage 10 10 20 60

CO-PO Matrix Plant Physiology TBBT-202

Course Outcome PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7 PSO1 PSO2 PSO3 PSO4

TBBT-202 CO 1. 2 - 2 1 2 - - 2 1 1 -

TBBT-202 CO 2. 3 2 2 2 2 - - 2 2 2 -

TBBT-202 CO 3. 2 - 1 2 2 - - 2 1 2 -

TBBT-202 CO 4. 2 - 3 2 2 - 2 2 2 2 2

Average CO

(TBBT-202)

2.2 2.0 2 1.7 2 - 2 2 1.5 1.7 2

Page 47: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc (H) Biotechnology Programme Code 05

Course Code PBBT-202 Credit 2

Year/Sem 1/2 L-T-P 0-0-4

Course Name Plant Physiology Lab

Objectives of the Course:

1. To provide students basic understanding of various physiological processes in plants.

2. To provide basic knowledge of various organs in plants through representation slides relatedto

monocot, dicot, root, stem and male and female gametophyte.

3. To develop a deeper understanding of various photosynthetic pigments present in plant leafand

its quantitative estimation.

4. To learn about the growth and development in plants and to have basic knowledge of plant

hormones, their role and function in relation to plants.

PRACTICALS

1. Separation of photosynthetic pigments by paper chromatography.

2. Quantitative estimation of cholorophyll a in the plant leaf.

3. Quantitative estimation of cholorophyll b in the plant leaf.

4. To study about plant growth hormones

5. Representation of slides related to monocot, dicot root and stem; male and female

gametophyte.

Course Outcomes (COs)

PBBT-202 CO 1Acquire skills about techniques related to plant physiology to prepare for higher

studies and employment.

PBBT-202 CO 2 Depict ability to identify and estimate various photosynthetic pigments found in

leaf and its application in botanical studies.

PBBT-202 CO 3 Identify and appraise concept and mechanism of photosynthesis in plants.

PBBT-202 CO 4 Comprehend different process of scarification, role of different plant hormone in

growth and development of the plants and application of same in agriculture

References:

Books

1. Bajracharya, D., Experiments in Plant Physiology: A Laboratory Manual, Narosa Publishing

House, 1999.

Mode of Evaluation

Internal (MM-25) External (MM-25)

Component Internal End Semester Examination

Weightage 25 25

Page 48: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

CO-PO Matrix Plant Physiology LabPBBT-202

Course Outcome PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PSO

1

PSO

2

PSO

3

PSO

4

PBBT-202 CO 1. 3 - 2 2 2 - - 2 2 2 2

PBBT-202 CO 2. 2 - 3 1 1 - - 2 2 1 -

PBBT-202 CO 3. 2 - 2 1 1 - - 2 1 1 -

PBBT-202 CO 4. 3 - 2 1 2 - - 2 2 2 -

Average CO (PBBT-

202) 2.5 - 2.2 1.2 1.5 - - 2 1.7 1.5 2

Page 49: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc (H) Biotechnology Programme Code 05

Course Code TBBT-203 Credit 4

Year/Sem 1/2 L-T-P 4-0-0

Course Name Developmental Biology

Objectives of the Course:

1.To provide fundamental aspects of embryology with detailed basics of organization and

differentiation.

2.To understand the morphological and structural details of blastulation, gastrulation stages and

types in embryonic development, organogenesis- neural development of embryo, fate of germ

layers.

3.To understand the basic concepts of cellular, molecular and genetic mechanism that affect

embryonic development.

4.To study the embryonic development mapping or Fate maps for embryo development and

organization of extra embryonic membranes and placenta.

UNIT I (Total Topics-8 and Hrs-10)

Gametogenesis and Fertilization:Definition, scope & historical perspective of development

Biology, Gametogenesis–Spermatogenesis, Oogenesis. Fertilization - Definition, mechanism, types

of fertilization. Different types of eggs on the basis of yolk.

UNIT II (Total Topics -10 and Hrs-10)

Early embryonic development:Cleavage: Definition, types, patterns & mechanism Blastulation:

Process, types & mechanism Gastrulation: Morphogenetic movements– epiboly, emboly,

extension, invagination, convergence, de-lamination. Formation & differentiation of primary germ

layers, Fate Maps in early embryos.

UNIT- III (Total Topics -10 and Hrs-10)

Embryonic Differentiation: Differentiation, Cell commitment and determination- the epigenetic

landscape: a model of determination and differentiation, control of differentiation at the level of

genome, transcription and post-translation level Concept of embryonic induction: Primary,

secondary & tertiary embryonic induction, Neural induction and induction of vertebrate lens.

UNIT-IV (Total Topics -8 and Hrs-10)

Organogenesis: Neurulation, notogenesis, development of vertebrate eye. Fate of different primary

germlayers Development of behaviour: constancy & plasticity, Extra embryonic membranes,

placenta in Mammals.

Course Outcomes (COs)

TBBT-203 CO 1.Comprehensive, detailed understanding of the animal embryonic development

and concepts of developmental biology, genetic models to provide insight into cellular and

molecular mechanisms for the development.

TBBT-203 CO 2.Perceptive of the animal development biology, evolutionary mechanisms and

relevant diseases and genetic disorder concepts.

TBBT-203 CO 3. Study the epigenetic and environmental impact on growth and development, and

the application of these technologies in different R&D sectors and industries.

TBBT-203 CO 4.Rationalization of the genetic studies, gene expression and cell-to-cell

interactions of animal models in embryonic development.

References:

Page 50: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Books

1. Gilbert S. F., Developmental Biology, Sinauer Associates, Inc. Publishers, 2006, VIII Edition.

2. BalinskyB.I., An introduction to Embryology, International Thomson Computer Press, 2008.

3. KalthoffA., Analysis of Biological Development, McGraw-Hill Professional, 2000, II Edition.

Mode of Evaluation

Internal (MM-40) External (MM-60)

Component Sessional -1 Sessional -2 Assignments End Semester Examination

Weightage 10 10 20 60

CO-PO Matrix Developmental Biology TBBT-203

Course Outcome PO1 PO2 PO3 PO4 PO5 PO6 PO7 PSO1 PSO2 PSO3 PSO4

TBBT-203 CO 1. 2 - - - - - 2 3 - - 1

TBBT-203 CO 2. 1 2 - - - - - - - - -

TBBT-203 CO 3. - - 1 - 2 - - - 1 2 -

TBBT-203 CO 4. - - - 1 - - - - - - 1

Average CO

(TBBT-203) 1.5 2 1 1 2 - 2 3 1 2 1

Page 51: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc (H) Biotechnology Programme Code 05

Course Code PBBT-203 Credit 1

Year/Sem 1/2 L-T-P 0-0-2

Course Name Developmental Biology Lab

Objectives of the Course:

1. To study the cell division, karyotyping, and embryonic development of insects andmammals.

2. To learn about the gonads and gametogenesis in insects and mammals.

3. To develop the practical skill sample handling, preparation of permanent cell/tissue mounts.

4. To expertise in analysis of cellular and embryonic development.

PRACTICALS

1. Identification of developmental stages of chick/ frog embryo using permanent mounts

2. Study of permanent mount of section of Insect Gonads.

3. Study of permanent mount of section of Ovary & Testis

4. Study of Oogenesis in Chick/Frog

5. Study of Spermatogenesis in Chick/Frog

6. Study of the developmental stages of Drosophila from permanent mounts.

7. Study of permanent mount of different types of placenta.

8. Study of permanent mount of Human Karyotype

9. To observe development of embryo in egg.

10. To enlist the genes related to embryogenesis and development.

Course Outcomes (COs)

PBBT-203 CO 1.Knowledge of cell growth, division, differentiation and embryonic development

and significance of same in biological research.

PBBT-203 CO 2. Practical understanding of cell division,formation of gamete and

embryonicdevelopment.

PBBT-203 CO 3. Perfection in handling and preparation of biological samples such as cells

culture,developing embryos.

PBBT-203 CO 4. Data analysis and perception of biological samples.

References:

Books

1. Key Experiments in Practical Developmental Biology, Manuel Marí-Beffa, Jennifer Knight

Cambridge University Press.

2. Developmental Biology Protocols, Rocky S. Tuan, Cecilia W. Lo, Springer Science &

BusinessMedia, Volume 1 (ISBN: 089603-574-3)

Mode of Evaluation

Internal (MM-25) External (MM-25)

Component Internal End Semester Examination

Weightage 25 25

Page 52: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

CO-PO Matrix Developmental Biology LabPBBT-203

Course Outcome PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7 PSO1 PSO2 PSO3 PSO4

PBBT-203 CO 1. 2 - - - - - 2 2 - - -

PBBT-203 CO 2. - 1 3 - 3 - - - 2 - -

PBBT-203 CO 3. - - 1 - 2 - - - 2 1 -

PBBT-203 CO 4. - - 2 1 - - - - 2 - 1

Average CO

(PBBT-203) 2 1 2 1 2.5 - 2 2 2 1 1

Page 53: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc (H) Biotechnology Programme Code 05

Course Code TBEC-204 Credit 2

Year/Sem 1/2 L-T-P 2-0-0

Course Name English Communication

Objectives of the Course:

1. To promote study, research and application of humanistic and scientific principle of

communication.

2. To enable the students to acquire proficiency, both in spoken (oral) and written language.

3. To develop comprehension skills, improve vocabulary, use proper grammar.

4. To acquire writing skills, correspond with others and enhance skills in spoken English.

UNIT I (Total Topics-10 and Hrs-5)

Communication Skills:Communication – Definition, Process of communication, Types of

communication—Verbal and Non-Verbal communication, Formal and InformalCommunication

(grapevine) and their significance, Barriers to spoken and listening Communication—Semantic

barriers, Physical barriers, Psychological barriers, Interpersonal barriers and Organizational

barriers.

UNIT II (Total Topics -7 and Hrs-5)

Grammar& Vocabulary:Antonyms, Synonyms and Homophones, One word substitution, Technical

Terms, Parts of Speech, Direct and Indirect Speech, Active and Passive Voice, Common

Grammatical Errors

UNIT- III (Total Topics -8 and Hrs-10)

Reading Skills & Listening skill: Reading Skills ,Meaning, Process of Reading and Importance of

Reading, Methods of improving Reading Skills, Development of thematic and stylistic skills of

comprehension in literature based readings:Poem: “Stopping by the woods on a snowy evening”

by Robert Frost, Story: “The Eyes are not here” by Ruskin Bond, Play: “Silence, The Court is

in Session” by Vijay Tendulkar Listening Skills: Listening : meaning; process; importance, Active

and Passive Listening, Types of Listening: Academic Listening, Appreciative Listening, Attentive

Listening, Methods for improving Listening Skills, Listening for note taking and drawing

inferences

UNIT-IV (Total Topics -6 and Hrs-10)

Writing skills: Précis Writing, Meaning, Process of Preparing a Technical Document, Business

Letters, Job Application Letter and Resume Writing, Report Writing: Structure, Types, Uses,

Proposal Writing: Structure, Types, Uses.

UNIT-V (Total Topics -7 and Hrs-5)

Speaking Skills: Importance of Speaking Skills, Methods and Objectives for Improving Speaking

Skills, Organs of speech, Mechanism of Speech, Phonetics: Classification of English Sounds,

Vowel, Consonants, Dip thongs, Phonemes, Allophones, Phonetic transcription, Stress, rhythm,

intonation: Rising Tone, Falling Tone and Rising-Falling tone, Public Speaking Skills, Features of

Effective Speech.

Course Outcomes (COs)

TBEC-204CO 1.Understand the meaning and the process of communication along with its types

and barriers.

TBEC-204CO 2.Develop proficiency in English Language through vocabulary building and

Page 54: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

correct use of grammar.

TBEC-204CO 3.Acquire competency in reading and listening by understanding the skills involved

and assessing & analyzing literary texts critically.

TBEC-204CO 4.Form a clear concept of writing style in technical communication and develop

technical writing skills.

TBEC-204CO 5. Develop speaking skills by understanding the basic concepts and enhance

proficiency in verbal and non- verbal communication

References:

Books

1. Sasikumar, V and Dhamija, P.V., Spoken English ,Tata McGraw Hill,2007 , 2nd Edition

2. Aslam, M.,Introduction of English Phonetics and Phonology, Cambridge, 2007.1st Edition

3. Revathi S ,Communicating Effectively,Abhishek Publications

4. Pal and Rorualling ,Essentials of Business Communication ,Sultan Chand and Sons.

5. Kohli AL ,New Design English Grammar, Reading and Writing Skills ,Kohli Publishers

6. Raina MK ,Developing English Communication ,Orient Blackswan.

7. Mohan K and Banerji M ,Developing Communication Skills , MacMillan India

8. Sharma SD ,Communication Skills ,Natraj Publishing House, Karnal

9. Wren PC and Martin H ,High School Grammar and Composition ,S. Chand publication

Mode of Evaluation

Internal (MM-40) External (MM-60)

Component Sessional -1 Sessional -2 Assignments End Semester Examination

Weightage 10 10 20 60

CO-PO Matrix English CommunicationTBEC-204

Course Outcome PO1 PO2 PO3 PO4 PO5 PO6 PO7 PSO1 PSO2 PSO3 PSO4

TBEC-204CO 1. - - - - - 3 - - - - -

TBEC-204CO 2. - - - - - 3 - - - - -

TBEC-204CO 3. - - - - - 3 - - - - -

TBEC-204CO 4. - - - - - 3 - - - - -

TBEC-204CO 5. - - - - - 3 - - - - -

Average CO

(TBEC-204) - - - - - 3 - - - - -

Page 55: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc (H) Biotechnology Programme Code 05

Course Code ADM-205 Credit 3

Year/Sem 1/2 L-T-P 2-1-0

Course Name Biostatistics &Computational biology

Objectives of the Course:

1. To expose the student to fundamental properties and concepts of modern statistical theory.

2. To understand the statistical techniques of data collection, analysis and interpretation.

3. To introduce computational biology and computational analysis of biological data.

4. To develop analytic thinking to solve biological problems through bio-statistical techniques.

UNIT I (Total Topics-10 and Hrs-5)

Types of Data, Collection of data; Primary & Secondary data, Classification and Graphical

representation of Statistical data. Measures of central tendency and Dispersion. Measures of

Skewness and Kurtosis.

UNIT II (Total Topics -9 and Hrs-6)

Probability classical & axiomatic definition of probability, Theorems on total and compound

probability), Elementary ideas of Binomial, Poisson and Normal distributions. Correlation and

Regression. Emphasis on examples from Biological Sciences.

UNIT- III (Total Topics – 8 and Hrs-6)

Methods of sampling, confidence level, critical region, testing of hypothesis and standard error,

large sample test and small sample test. Problems on test of significance, t-test, chi-square test for

goodness of fit and analysis of variance (ANOVA)

UNIT-IV (Total Topics -8 and Hrs-5)

Computational biology: Basics of computer, input output devices, MS office, application of

computer programmes and software in the field of life science.

UNIT-V (Total Topics -7 and Hrs-5)

Microsoft word:- concept of toolbar ,character ,paragraph & document formatting. Microsoft

excel:-concept of spread sheets, creating worksheet, well formatted documents, concept of row ,

column ,cell & formula bar. Microsoft power point:- slide presentation, slide layout & design.

Course Outcomes (COs)

ADM-205CO 1.Demonstrate an understanding of the central concepts of modern statistical theory

and their probabilistic foundation.

ADM-205CO 2.Collect data relating to variable/variables which will be examined and calculate

descriptive statistics from these data. Further identify convenient sample by using sampling theory.

ADM-205CO 3. Perform computational analyses of biological datasets and relate the results to

core principles in biology. And apply biostatistical knowledge to real-life problems in biological

research.

ADM-205CO 4.Apply computational methods to help execute a biological research plan and

Analyze biological problems from global and ethical impact perspectives (impact of computational

biology methods).

References:

Books

1. Banerjee PK ,Introduction to biostatistics , S Chand Publication.

Page 56: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

2. Antonisamy B, Kumar P and Christopher S ,Principle and practice of biostatistics, Elsevier.

3. Haubold B.,Introduction to Computational Biology: An Evolutionary Approach ,Birkhduser

publication.

4. Sinha PK and Sinha P ,Computer fundamentals ,BPB publication.

Mode of Evaluation

Internal (MM-40) External (MM-60)

Component Sessional -1 Sessional -2 Assignments End Semester Examination

Weightage 10 10 20 60

CO-PO Matrix Biostatistics & Computational Biology ADM-205

Course Outcome PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7 PSO1 PSO2 PSO3 PSO4

ADM-205CO 1. 3 3 1 - - - 2 3 1 - 1

ADM-205CO 2. 3 3 3 - 2 - 2 2 3 2 2

ADM-205CO 3. 3 3 3 - 2 - 2 2 3 3 2

ADM-205CO 4. 3 2 3 1 2 - 2 2 2 2 2

Average CO

(ADM-205) 3 2.7 2.5

1 2

- 2 2.2 2.2 2.3 1.7

Page 57: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc (H) Biotechnology Programme Code 05

Course Code ADP-206 Credit 1

Year/Sem 1/2 L-T-P 0-0-2

Course Name Seminar

Objectives of the Course:

1. To acquire skills for current trends in biotechnology and life sciences and their future

prospects.

2. To enhance communicational, presentation and scientific writing skills, ability to work in

team by substantive participation in seminar, workshop, oral and poster presentation.

3. To acquire ability to utilize and implement modern tools and techniques for data

compilation, presentation and analysis.

Guidelines

1. A topic of seminar related to biotechnology filed will be assigned to all students by the

faculty member.

2. Students have to give a presentation in the form of PPT in seminar hall submit a hard copy.

3. Evaluation will be done on the basis of presentation and submission of report on assigned

topic to the faculty.

Course Outcomes (COs)

ADP-206 CO 1.Acquaintance about the current trends in biotechnology and life sciences and

their future prospects.

ADP-206 CO 2.Enhance communicational, presentation and scientific writing skills, ability to

work in team by substantive participation in seminar, workshop, oral and

poster presentation.

ADP-206 CO 3. Acquire ability to utilize and implement modern tools and techniques for data

compilation , presentation and analysis.

Mode of Evaluation

Internal (MM-50)

Component Internal

Weightage 50

CO-PO Matrix SeminarADP-206

Course Outcome PO1 PO2 PO3 PO4 PO5 PO6 PO7 PSO1 PSO2 PSO3 PSO4

ADP-206 CO 1. - - - - - 3 - - - - -

ADP-206 CO 2. - - - - - 3 - - - - -

ADP-206 CO 3. - - - - 1 3 - - - 1 -

Average CO

(ADP-206) 3 2.7 2.5

1 2

- 2 2.2 2.2 2.3 1.7

Page 58: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

SEMESTER- III

Page 59: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc (H) Biotechnology Programme Code 05

Course Code TBBT-301 Credit 4

Year/Sem 2/3 L-T-P 4-0-0

Course Name Genetics

Objectives of the Course:

1. To provide fundamental aspects of genetics with detailed basics of chromosomal organization

and sequential features.

2. To study about the morphological and structural details of chromosomes.

3. To study about the genes and their interaction, role in inheritance; role of mutations and sex

determination.

4. To understand and learn about extra chromosomal genetic material, viral genetics and their role

in testing biological samples, genome fingerprinting, mapping and pedigree analysis and

testing.

UNIT I (Total Topics-10 and Hrs-10)

Introduction: Genetics & heredity. Mendelian genetics: monohybrid, di-hybrid and tri hybrid

crosses, Law of segregation & Principle of independent assortment. Test and back crosses,

Chromosomal theory of inheritance, Allelic interactions: Concept of dominance, incomplete

dominance, co-dominance, pleiotropy, multiple allele, pseudo-allele, essential and lethal genes,

penetrance and expressivity. Non allelic interactions: Complementary genes, Epistasis (dominant

& recessive), duplicate genes and inhibitory genes.

UNIT II (Total Topics-9 and Hrs-10)

Genetic organization of prokaryotic and viral genome. Chromosome and genomic organization of

eukaryotes: unique & repetitive DNA, satellite DNA. Centromere and telomere DNA sequences,

VNTRs, transposons, noncoding DNA. Structure and characteristics of bacterial and eukaryotic

chromosome, chromosome morphology, packaging, karyotype, giant chromosomes.

UNIT- III (Total Topics-11 and Hrs-8)

Chromosome and gene mutations: Definition and types of mutations, causes of mutations,

chromosomal aberrations in human beings.

Sex determination and sex linkage: Mechanisms of sex determination, Environmental factors and

sex determination, sex differentiation, Barr bodies, dosage compensation, genetic balance theory,

Fragile-X-syndrome and chromosome, sex influenced dominance, sex limited gene expression, sex

linked inheritance.

UNIT-IV (Total Topics-7 and Hrs-8)

Genetic linkage, crossing over and chromosome mapping. Introduction to Pedigree analysis.

Extra chromosomal inheritance: maternal effects, maternal inheritance, cytoplasmic inheritance,

genomic imprinting. Basics of bacteriophage genetics: Lytic cycle & Lysogenic cycle.

Course Outcomes (COs)

TBBT-301CO 1.Comprehensive understanding of the heredity, heritable traits and genetic

methodology or laws.

TBBT-301CO 2.Provides insight into cellular and molecular mechanisms of the inheritance of

traits.

TBBT-301CO 3. Proficiency to design, implement and analyze the results at statistically of animal

Page 60: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

and plant model systems

TBBT-301CO 4.Perceptive of the genetic concepts on societal, environmental issues and genetic

technologies in different R&D sectors and industries.

References:

Books

1. Gardner, E.J., Simmons, M.J., and Snustad, D.P., Principles of Genetics, John Wiley & Sons,

2006, 8thedition.

2. Snustad, D.P. and Simmons, M.J., Principles of Genetics., John Wiley and Sons Inc. ,2009 ,5th

edition.

3. Brown, T.A., Introduction to Genetics: A Molecular Approach, Oxford University Press.

4. Russell, P. J., Genetics- A Molecular Approach, Benjamin Cummings.,2009, 3rdedition.

5. Griffiths, A.J.F., Wessler, S.R., Lewontin, R.C. and Carroll, S.B., Introduction to Genetic

Analysis,, W. H. Freeman & Co., 9thedition.

6. Verma, R.S., Cell Biology, Genetics, Molecular Biology Evolution and Ecology.

Mode of Evaluation

Internal (MM-40) External (MM-60)

Component Sessional -1 Sessional -2 Assignments End Semester Examination

Weightage 10 10 20 60

CO-PO Matrix Genetics TBBT-301

Course Outcome PO1 PO2 PO3 PO4 PO5 PO6 PO7 PSO1 PSO2 PSO3 PSO4

TBBT-301CO 1. 3 - - - - - 2 3 - - 1

TBBT-301CO 2. 1 2 - - - - - - - - -

TBBT-301CO 3. - - 1 - 2 - - - 1 -

TBBT-301CO 4. - - - 1 - - - - - 2 -

Average CO

(TBBT-301) 2 2 1 1 2 - 2 3 1 2 1

Page 61: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc (H) Biotechnology Programme Code 05

Course Code PBBT-301 Credit 2

Year/Sem 2/3 L-T-P 0-0-4

Course Name Genetics Lab

Objectives of the Course:

1. To study the cell division, karyotyping, chromosome and mendelian genetic cross.

2. To learn about the pattern of inheritance, pedigree analysis and genome mapping, the types

of mutation and their effect on genetic material.

3. To develop the practical skill and expertise in handling of biological samples, preparation of

permanent/temporary mounts and their analysis.

4. To expertise in pedigree chart data analysis and estimation of inheritance pattern.

PRACTICALS

1. Study of permanent and temporary mount of mitosis.

2. Study of permanent and temporary mount of meiosis.

3. Mendelian deviations in dihybrid crosses.

4. Karyotyping with the help of photographs.

5. Pedigree charts of blood group & color blindness

6. Demonstration / study of bacterial conjugation.

7. Demonstration / study of bacterial transformation.

8. Demonstration of Barr body.

9. Study of Mutation by radiations/ chemicals.

Course Outcomes (COs)

PBBT-301CO 1.Practical Knowledge of cell division and formation of gametic cells, mendelian

cross, genetic material and transfer of genes

PBBT-301CO 2. Practical knowledge of the methods for measurement and their quantitative as

well as qualitative estimation and their application in research.

PBBT-301CO 3. Expertise in instrument and sample handling for the preparation and analysis of

samples.

PBBT-301CO 4. Proficiency in data analysis and perception of biological samples such as cells,

genome to study the heredity.

References:

Books

1. Gardner, E.J., Simmons, M.J., and Snustad, D.P., Principles of Genetics, John Wiley & Sons,

2006, 8thedition.

2. Snustad, D.P. and Simmons, M.J., Principles of Genetics., John Wiley and Sons Inc. 2009 ,5th

edition.

3. Brown, T.A., Introduction to Genetics: A Molecular Approach, Oxford University Press.

4. Russell, P. J., Genetics- A Molecular Approach, Benjamin Cummings.,2009 ,3rdedition.

5. Griffiths, A.J.F., Wessler, S.R., Lewontin, R.C. and Carroll, S.B., Introduction to Genetic

Analysis,, W. H. Freeman & Co., 9thedition.

6. Verma, R.S., Cell Biology, Genetics, Molecular Biology Evolution and Ecology.

Page 62: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Mode of Evaluation

Internal (MM-25) External (MM-25)

Component Internal End Semester Examination

Weightage 25 25

CO-PO Matrix GeneticsLab PBBT-301

Course Outcome PO1 PO2 PO3 PO4 PO5 PO6 PO7 PSO1 PSO2 PSO3 PSO4

PBBT-301CO 1. 2 - - - - - 2 2 - - -

PBBT-301CO 2. - - 3 - 3 - - - 2 - -

PBBT-301CO 3. - - 2 - 2 - - - 1 3 -

PBBT-301CO 4. - 1 2 1 - - - - 3 - 1

Average CO

(PBBT-301) 2 1 2.3 1 2.5 - 2 2 2 3 1

Page 63: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc (H) Biotechnology Programme Code 05

Course Code TBBT-302 Credit 4

Year/Sem 2/3 L-T-P 4-0-0

Course Name General Microbiology

Objectives of the Course:

1. To acquire the theoretical knowledge about classification of the microorganisms and their

control.

2. To impart knowledge of the basic principles of bacterial reproduction, essential microbes in

food, nature of pathogenic microorganisms, laboratory diagnosis, transmission, prevention

and control of diseases.

3. To appraise and comprehend the numerous modern tools and techniques and learn about

Bioenergetics, Nucleic Acid metabolisms, food microbiology etc to get well versed with

microbes better and understand our world, our place within it.

4. To inculcate the advanced approaches used in various fields of allied sciences and encourage

students to choose a definite career with the area of their interest.

5. To acknowledge the diversity and activity of microorganisms in their natural environment,

their mutual interactions and their survival and adaptation strategies.

UNIT I (Total Topics-9 and Hrs-10)

Classification of microorganism: Classification of different type of microorganisms. Nutritional

classification of Bacteria. Unique features of Prokaryotes and eubacteria. Temperature-

(psychrophiles,mesophiles,thermophiles,

extremophiles,thermodurics,psychrotrophs),pH(acidophiles,alkaliphiles),soluteandwateracti

vity

(halophiles,xerophiles,osmophilic),Oxygen(aerobic,anaerobic,microaerophilic,facultativeaer

obe, facultative anaerobe), barophilic.

UNIT II(Total Topics-8 and Hrs-10)

Microbial growth: Bacterial culture: Synchronous, Batch & continuous culture, measurement of

growth and factors affecting growth of bacteria.

Microbialgrowthinresponsetonutritionandenergy–Autotroph/Phototroph,heterotrophy,

Chemolithoautotroph, Chemolithoheterotroph, Chemoheterotroph, Chemolithotroph,

photolithoautotroph,Photoorganoheterotroph.

UNIT- III (Topics-9and Hrs-8)

Bacterial Reproduction: Transformation - Discovery, mechanism of natural competence

Conjugation - Discovery, mechanism, Hfr and F’ strains Transduction - Generalized

transduction, specialized transduction

UNIT-IV (Total Topics-6 and Hrs-8)

Control of Microorganisms: Control of microorganisms by physical, chemical and

chemotherapeutic Agents. Water Microbiology: Major bacterial pollutants of water and its

disposal.

UNIT-V (Total Topics-9 and Hrs-10)

Food Microbiology: Important microorganism in food Microbiology: Moulds, Yeasts, bacteria.

Major food born infections and intoxications, Fermented Foods.Miocatalysts from extreme

Page 64: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

thermophilic and hyperthermophilic archaea and bacteria

Course Outcomes (COs)

TBBT-302 CO1. Acquire knowledge about different types of microorganism, characteristic

features reproduction and environmental factors influencing their growth.

TBBT-302 CO2. Comprehend different methods of culturing microbes measurement and

analysis of bacterial growth.

TBBT-302 CO3. Understand various techniques utilized for control of microorganisms

alongwith identification of bacterial water pollutant.

TBBT-302 CO4 Identify microbes utilized in food microbiology processes ad their respective

impact on health and environment.

References:

Books

1. Chatterjea& Shinde, Jaypee publications, Textbook of Medical Biochemistry

2. Madigan, M.T., Martinko, J.M. and Parker J.B., Biology of Microorganisms, Pearson,

Benjamin Cummings,2009 12th edition.

3. Pelczar, M.J., Chan, E.C.S. and Krieg, N.R., Microbiology, McGraw Hill Book Company,

1993 5th edition.

4. Stanier ,R.Y., Ingraham, J.L., Wheelis, M.L. and Painter, P.R., General Microbiology,

McMillan,2005 5th edition

5. Willey, J.M., Sherwood, L.M, and Woolverton CJ. Prescott, Harley and Klein’s,

Microbiology., McGraw Hill Higher Education, 2008, 7th edition.

Mode of Evaluation

Internal (MM-40) External (MM-60)

Component Sessional -1 Sessional -2 Assignments End Semester Examination

Weightage 10 10 20 60

CO-PO Matrix General Microbiology TBBT-302

Course Outcome PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7 PSO1 PSO2 PSO3 PSO4

TBBT-302CO 1. 2 1 - - - - 1 2 - - 1

TBBT-302CO 2. - - 1 1 - - - - - 1 -

TBBT-302CO 3. - - - 1 2 - - - 1 1 -

TBBT-302CO 4. - - 1 1 1 - - - - - 1

Average CO

(TBBT-302) 2 1 1 1 1.5 - 1 2 1 1 1

Page 65: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc (H) Biotechnology Programme Code 05

Course Code PBBT-302 Credit 2

Year/Sem 2/3 L-T-P 0-0-4

Course Name General Microbiology Lab

Objectives of the Course:

1. To exhibit technical skill for isolation bacterial from various samples, interpret data to derive

a conclusion pertaining to microbial load of sample analyzed.

2. To identify principle and procedure of staining methods in the laboratory.

3. To utilize various techniques for study, enumerate microorganisms and interpret results.

4. To conduct experiments related to biochemical activities of bacteria, record and analyze

observations

PRACTICALS

1. Isolation of bacteria from water, air, skin, teeth samples etc& their biochemical

characterization.

2. Different methods of Isolation of pure culture.

3. Staining methods: Simple staining of bacterial pure culture.

4. Staining methods: Gram staining of bacterial pure culture.

5. Staining methods: Bacterial capsule/spore staining.

6. Staining methods: Negative staining.

7. Staining methods: Hanging drop method of staining.

8. Demonstration of starch hydrolysis by bacterial cultures.

9. Determination of bacterial cell size by micrometry.

10. Enumeration of microorganism

Course Outcomes (COs)

PBBT-302 CO 1.Exhibit technical skill to isolate bacterial from various samples, interpret data

to derive a conclusion pertaining to microbial load of sample analyzed.

PBBT-302 CO 2. Identify principle and procedure of staining methods and respective

application in biological research.

PBBT-302 CO 3. Utilization of various techniques to study, enumerate microorganisms and

interpret results.

PBBT-302 CO 4. Conduct experiments related to biochemical activities of bacteria, record and

analyze observations

References:

Books

1. Chatterjea& Shinde, Jaypee publications, Textbook of Medical Biochemistry

2. Madigan, M.T., Martinko, J.M. and Parker J.B., Biology of Microorganisms, Pearson,

Benjamin Cummings,2009,12th edition.

3. Pelczar, M.J., Chan, E.C.S. and Krieg, N.R., Microbiology, McGraw Hill Book Company,

1993 ,5th edition.

4. Stanier ,R.Y., Ingraham, J.L., Wheelis, M.L. and Painter, P.R., General Microbiology,

McMillan,2005, 5th edition

5. Willey, J.M., Sherwood, L.M, and Woolverton CJ. Prescott, Harley and Klein’s,

Microbiology., McGraw Hill Higher Education, 2008, 7th edition.

Page 66: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Mode of Evaluation

Internal (MM-25) External (MM-25)

Component Internal End Semester Examination

Weightage 25 25

CO-PO Matrix General Microbiology Lab PBBT-302

Course Outcome PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7 PSO1 PSO2 PSO3 PSO4

PBBT-302 CO 1. 1 - - 1 1 - 1 2 - - 1

PBBT-302 CO 2. 1 - 1 - - - - 1 - 1 -

PBBT-302 CO 3. - - - - 1 - - - 1 - -

PBBT-302 CO 4. - - 1 - - - - - - 1 -

Average CO

(PBBT-302) 1 - 1 1 1 - 1 1.5 1 1 1

Page 67: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc (H) Biotechnology Programme Code 05

Course Code TBBT-303 Credit 4

Year/Sem 2/3 L-T-P 4-0-0

Course Name Chemistry-I

Objectives of the Course:

1. To instill graduate about the concept of shape of different orbitals, filling of electrons and

electronic configuration these are the basis of inorganic concepts, which are useful topics for

higher level entrance exams.

2. To understand the students about fundamental concepts of general organic chemistry,

reactive intermediates as well as isomerism and aromaticity.

3. To make them understand the reaction mechanism by the attack of electrophile and

nucleophiles.

4. To appraise various concentration terms and to develop skills in handling of apparatus and

chemicals it will be helpful to students if are interested in industries

UNIT I (Total Topics-12 and Hrs-10)

General Inorganic and Chemical Bonding: Concpet of atomic structure, de-Broglie’s relation,

Heisenberg Uncertainty principle. Quantum numbers, Significance of quantum numbers, Shapes

of s, p and d atomic orbitals, nodal planes. Rules for filling electrons in various orbitals,

Electronic configurations of the atoms. Stability of half-filled and completely filled orbitals.

Periodic table-Idea about periods and groups. Definition and trend of Ionisation Energy,

Electron affinity, Electronegativity & atomic size in periodic table.Polarizing power and

polarizability. Fajan’s rules, ionic character in covalent compounds, bond moment, dipole

moment andpercentage ionic character.

UNIT II (Total Topics-10 and Hrs-10)

Fundamentals of Organic Chemistry:Physical Effects, Electronic Displacements: Inductive

Effect, Electromeric Effect, Resonance and Hyperconjugation. Cleavage of Bonds: Homolysis

and Heterolysis.Structure, shape and reactivity of organic molecules: Nucleophiles and

electrophiles.

Reactive Intermediates: Carbocations, Carbanions and free radicals. Strength of organic acids

and bases: Comparative study with emphasis on factors affecting pK values. Aromaticity:

Benzenoids and Hückel’s rule.

UNIT- III (Total Topics-10 and Hrs-10)

Isomerism:Definition of isomerism, structural isomerism and its types( Chain, position,

functional, meta merism and tautaomerism) with example. Conformations with respect to ethane,

butane and cyclohexane. Interconversion of Wedge Formula, Newmann, Sawhorse and Fischer

representations. Stereo isomerism- Concept of chirality (upto two carbon atoms). Configuration:

geometrical and Optical isomerism; Enantiomerism, Diastereomerism and Meso compounds).

Threo and erythro; D and L; cis – trans nomenclature; CIP Rules: R/ S (for upto 2 chiral carbon

atoms) and E / Z Nomenclature (for upto two C=C systems).

UNIT-IV (Total Topics-8 and Hrs-5)

Qualitative and quantitative analysis, volumetric and gravimetric analysis. principle of

volumetric analysis. Concept of pH, buffer, Henderson equation. Concept of strength and

concentration of solution, Normality, Molarity, Molality and interconversion of strength.

Page 68: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

UNIT-V (Total Topics-7 and Hrs-5)

Volumetric Analysis:Types of volumetric analysis. Acid-base, complexometric, redox and

precipitation titration. Oxidation number and calculation of oxidation number in the compounds.

Concept of oxidation and reduction reactions.

Course Outcomes (COs)

TBBT-303 CO1. Apply the scientific knowledge of electronic configuration, periodic properties

of elements in each group in the periodic tables, analyse the problems with the help of analytical

knowledge related to industry or Society or health and communicate effectively

TBBT-303 CO2. Impart essential theoretical knowledge on atomic structure, periodic properties,

Chemical bonding, isomerism, stereochemistry for scientific problems and demonstrate the

knowledge to applied sciences for lifelong learning.

TBBT-303 CO3. Develop skills for quantitative estimation using the different branches of

Volumetric analysis, statistical knowledge with life long learning skills in society and industry

and also inculcate habit of working in a team

TBBT-303 CO4. Know, recall and explain the fundamental principles of chemistry that include

General Inorganic chemistry, Fundamental organic Chemistry and learning of analytical

laboratory learning skill for the analysis and interpretation of data for the professional ethics.

References:

Books

1. Vogel, A.I., Vogel’s Qualitative Inorganic Analysis, Prentice Hall, 7th Edition.

2. Vogel, A.I., Vogel’s Quantitative Chemical Analysis, Prentice Hall, 6th Edition.

3. Vogel, A.I. Textbook of Practical Organic Chemistry, Prentice Hall, 5th Edition.

4. Mann, F.G. and Saunders, B. C., Practical Organic Chemistry, Orient Longman, 1960.

5. Morrison, R. N. and Boyd R.N, Organic Chemistry, Dorling Kindersley (India) Pvt. Ltd.

(Pearson Education).

Mode of Evaluation

Internal (MM-40) External (MM-60)

Component Sessional -1 Sessional -2 Assignments End Semester Examination

Weightage 10 10 20 60

CO-PO Matrix Chemistry-I TBBT-303

Course Outcome PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PSO

1

PSO

2

PSO

3

PSO

4

TBBT-303 CO 1. - 2 2 - - - 1 2 - - 1

TBBT-303 CO 2. - 2 2 - - - 2 2 - - 1

TBBT-303 CO 3. - 1 2 - - - 2 2 1 - 1

TBBT-303 CO 4. - 2 2 - - - 1 2 1 - 1

Average CO (TBBT-

303) - 1.7 2 - - - 1.5 2 1 - 1

Page 69: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc (H) Biotechnology Programme Code 05

Course Code PBBT-303 Credit 1

Year/Sem 2/3 L-T-P 0-0-2

Course Name Chemistry-I Lab

Objectives of the Course:

1. To impart the students the knowledge on phase rule and its applications.The aim of the

Course is to make students familiar with various physical phenomena.

2. The course provides training in advanced physical chemistry laboratory techniques.

3. The course will give the introduction of titration, conductivity, dissociation and

buffersolution also their handling.

4. The overall purpose of course is to make students aware of how chemical process can be

designed, developed and run insustainable ways.

PRACTICALS

Volumetric Analysis

1. Estimation of sodium carbonate and sodium hydrogen carbonate present in a mixture.

2. Estimation of oxalic acid by titrating it with KMnO 4 .

3. Estimation of water of crystallization in Mohr’s salt by titrating with KMnO 4 .

4. Estimation of Fe (II) ions by titrating it with K 2 Cr 2 O 7 using internal indicator.

5. Estimation of Cu (II) ions iodometrically using Na 2 S 2 O 3 .

Organic Chemistry

1. Test of aromaticity

2. Detection of extra elements (N, S, Cl, Br, I) in organic compounds (containing upto two

extra elements).

Course Outcomes (COs)

PBBT-303 CO1. Understand and explain scientifically the various chemistry related problems in

industry.

PBBT-303 CO2. Develop an understanding of the role of the chemist in tasks employing

physicalchemistry.

PBBT-303 CO3. Ability to demonstrate the significance of Buffer solutions, knowledge of

electrochemistryas well as conductivity.

PBBT-303 CO4. Ability to analyze and generate experimental skills, this will help students in

employment

in industries.

References: Books

1.Pandey O.P, Bajpai D.N Practical Chemistry. S Chand Publication 2002

Page 70: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Mode of Evaluation

Internal (MM-25) External (MM-25)

Component Internal End Semester Examination

Weightage 25 25

CO-PO Matrix Chemistry-I Lab PBBT-303

Course Outcome PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PSO

1

PSO

2

PSO

3

PSO

4

PBBT-303CO 1. - 2 1 - - - 1 2 1 - 1

PBBT-303CO 2. - 1 1 - - - 1 1 1 - -

PBBT-303CO 3. - 1 1 - - - 1 2 2 - -

PBBT-303CO 4. - 2 1 - - - 1 1 - - 1

Average CO (PBBT-

303) - 1.5 1 - - - 1 1.5 1.3 - 1

Page 71: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc (H) Biotechnology Programme Code 05

Course Code TBBT-304 Credit 3

Year/Sem 2/3 L-T-P 3-0-0

Course Name Bioethics and Biosafety

Objectives of the Course:

1. To provide basic knowledge for development of moral and professional ethics in the field of

healthcare.

2. To develop ethical skills through world-wide case studies in healthcare and environment.

3. To provide scientific knowledge regarding safety principles to laboratory practices in which

potentially hazardous materials are manipulated or handled.

4. To give scientific knowledge regarding protection of inventions and technologies

UNIT I (Total Topics-10 and Hrs-10)

Introduction to Indian Patent Law. Intellectual property rights. Patents, Copyrights, Trademarks,

Trade secrets, Patenting of GMOs. Process of filing a patent: Process and product patent, patent

application and guidelines, Prerequisites of filing a patent, Novelty, Utility.

UNIT II (Total Topics-10 and Hrs-10)

Entrepreneurship: Selection of a product, line, design and development processes, economics on

material and energy requirement, stock the product and release the same for making etc. The basic

regulations of excise: Demand for a given product, feasibility of its production under given

constraints of raw material, energy input, financial situations export potential etc.

UNIT- III (Total Topics-5 and Hrs-7)

Bioethics – Guidelines, different paradigms of Bioethics – National & International.

Ethical issues against the molecular technologies. Release of Genetically modified Organisms and

Recombinant drugs, Regulating Agencies.

UNIT-IV (Total Topics-5 and Hrs-7)

Biosafety– Introduction to biosafety and health hazards concerning biotechnology. Introduction to

the concept of containment level and Good Laboratory Practices (GLP) and Good Manufacturing

Practices (GMP).

Course Outcomes (COs)

TBBT-304 CO1. Impart knowledge and skills to develop moral and professional ethics related to

healthcare as well as to become entrepreneurs.

TBBT-304 CO2. Development of skills about broader global ethical issues through case studies in

healthcare.

TBBT-304 CO3. Development of scientific knowledge regarding laboratory and manufacturing

practices for safety regulation in disposal of hazardous chemicals in the environment.

TBBT-304 CO4. Development of knowledge regarding protection of their inventions and

technologies

References:

Books

1. Sateesh, M.K., Bioethics and Biosafety, I. K. International Pvt Ltd, 2010.

2. Krishna, V .S., Bioethics and Biosafety in Biotechnology, New age international publisher, 2010.

Page 72: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Mode of Evaluation

Internal (MM-40) External (MM-60)

Component Sessional -1 Sessional -2 Assignments End Semester Examination

Weightage 10 10 20 60

CO-PO Matrix Bioethics and Biosafety TBBT-304

Course Outcome PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PSO

1

PSO

2

PSO

3

PSO

4

TBBT-304 CO 1. 2 - - 2 - - 1 2 - 1 -

TBBT-304 CO 2. 3 - - 2 - - - 1 2 2 1

TBBT-304 CO 3. 3 2 1 - - - - 2 - 1 -

TBBT-304 CO 4. 3 - - 1 - - - 2 1 1 1

Average CO (TBBT-

304) 2.7 2 1 1.6 - - 1 1.7 1.5 1.2 1

Page 73: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc (H) Biotechnology Programme Code 05

Course Code PBBT-304 Credit 1

Year/Sem 2/3 L-T-P 0-0-2

Course Name Bioethics and Biosafety Lab

Objectives of the Course:

1. To provide knowledge for filing patent in India.

2. To develop ethical skills through world-wide case studies in healthcare and environment.

3. To provide scientific knowledge regarding safety principles to laboratory practices in which

potentially hazardous materials are manipulated or handled through case study.

PRACTICALS

1. Proxy filing of Indian Product patent.

2. Proxy filing of Indian Process patent.

3.Case study on women health ethics.

4. Case study on medical errors and negligence.

5. Case study on handling and disposal of radioactive waste.

Course Outcomes (COs)

PBBT-304 CO 1.Impart knowledge and skills to develop moral and professional ethics related to

healthcare through various case studies.

PBBT-304 CO 2. evelopment of scientific knowledge regarding laboratory and manufacturing

practices for safety regulation in disposal of hazardous chemicals in the environment through

case Studies.

PBBT-304 CO 3. Demonstrate the process under IP India for filing of process and product

patent for the protection of their inventions and technologies.

PBBT-304 CO 4. Acquire knowledge regarding protection of inventions, publications and

technologies through case studies.

References:

Books

1. Sateesh, M.K., Bioethics and Biosafety, I. K. International Pvt Ltd, 2010.

2. Krishna, V .S., Bioethics and Biosafety in Biotechnology, New age international publisher,

2010.

Mode of Evaluation

External (MM-25)

Component End Semester Examination

Weightage 25

Page 74: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

CO-PO Matrix Bioethics and Biosafety Lab PBBT-304

Course Outcome PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PSO

1

PSO

2

PSO

3

PSO

4

PBBT-304 CO 1. 2 - - 1 - - 1 1 - 1 -

PBBT-304 CO 2. 2 - - 1 - - - 1 2 2 1

PBBT-304 CO 3. 2 1 1 - - - - 1 - 1 -

PBBT-304 CO 4. 2 - - 1 - - - 1 1 1 1

Average CO (PBBT-

304) 2 1 1 1 - - 1 1 1.5 1.2 1

Page 75: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc (H) Biotechnology Programme Code 05

Course Code TBBT-305 (I) Credit 3

Year/Sem 2/3 L-T-P 3-0-0

Course Name Enzymology

Objectives of the Course:

1. To provide the basic knowledge about enzymatic reactions and factors effecting it.

2. To develop and design applicable enzyme concept, enzyme isolation, crystallization,

immobilize enzymes and its applications.

3. To provide exposure to students about mechanism of enzyme actions and industrial use of

enzyme.

4. To promote research and Innovation in Enzymology research for benefit for human being.

UNIT I (Total Topics-8 and Hrs-10)

Isolation, crystallization and purification of enzymes. Zymogens and their activation (Proteases

and Prothrombin). Kinetics of enzyme activity: Different plots for the determination of Km and

Vmax and their physiological significance, factors affecting rate of enzyme catalyzed reactions.

UNIT II (Total Topics-10 and Hrs-10)

Enzyme inhibition: types of inhibition, determination of Ki, suicide inhibitor. Mechanism of

enzyme action: General mechanistic principle, Acid-Base, Nucleophilic and Covalent Catalysis.

Enzyme regulation: Product inhibition, feedback control, covalent modification.

Metalloenzymes, monomeric & oligomeric enzymes, activation energy and transition state,

enzyme activity, specific activity, common features of active sites, enzyme specificity: types &

theories.

UNIT- III (Total Topics-8 and Hrs-10)

Role of NAD+, NADP+, FMN/FAD, coenzymes A, Thiamine pyrophosphate, Pyridoxal

phosphate, lipoic-acid, Biotin vitamin B12, Tetrahydrofolate and metallic ions. Allosteric

enzymes & kinetics of allosteric enzymes. Isoenzymes– multiple forms of enzymes with special

reference to lactate dehydrogenase. Multienzyme complexes. Ribozymes. Multifunctional

enzyme-eg Fatty Acid synthase.

UNIT-IV (Total Topics-7 and Hrs-10)

Immobilized enzyme: Methods for immobilization of enzymes. Application of Immobilized and

soluble enzyme in health and industry. Enzyme engineering: Thermal stability and catalytic

efficiency of enzyme, site directed mutagenesis. Structure–function relationship of enzymes.

Course Outcomes (COs)

TBBT-305 (I) CO1. Explain the methods of enzyme isolation, crystallization, purification and

reactions of enzymes, zymogens and course of action of enzyme and kinetics.

TBBT-305 (I) CO2. Acquire knowledge related classification and enzyme inhibition, types of

inhibition, role of Ki in Acid Base catalysis.

TBBT-305 (I) CO3. Apply the contextual knowledge of isoenzymes to recognize their role in

clinical field with its types and reference to LDH.

TBBT-305 (I) CO4. Compare the different methods of enzyme immobilization and explore

theoretically these methods with industries.

References:

Page 76: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Books

1. Palmer & Bonner, Enzymes: Biochemistry, Biotechnology & Clinical Chemistry, Woodhead

Publishing.

2. Cornish-Bowde, A., Fundamentals of Enzyme Kinetics, Portland Press,2005.

Mode of Evaluation

Internal (MM-40) External (MM-60)

Component Sessional -1 Sessional -2 Assignments End Semester Examination

Weightage 10 10 20 60

CO-PO Matrix Enzymology TBBT-305 (I)

Course Outcome PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PSO1 PSO2 PSO3 PSO4

TBBT-305 (I) CO 1. 2 - 2 - - 2 - 2 - - -

TBBT-305 (I) CO 2. - 1 2 - - - - 2 - 2 -

TBBT-305 (I) CO 3. 2 - - - - 1 - 2 1 2 -

TBBT-305 (I) CO 4. - 2 3 - - - - 2 - 3 -

Average CO (TBBT-

305 (I))

2 1.5 2.3 - - 1.5 - 2 1 2.3 -

Page 77: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc (H) Biotechnology Programme Code 05

Course Code PBBT-305 (I) Credit 1

Year/Sem 2/3 L-T-P 0-0-2

Course Name Enzymology Lab

Objectives of the Course:

1. To understand the concept about various enzyme assays detection techniques, enzyme

applications in various medical, food and pharmaceutical industries.

2. To demonstrate the methods of enzyme isolation, crystallization, purification and reactions of

enzymes, zymogens and course of action of enzyme and kinetics.

3. To promote research and Innovation in enzymes research for benefit for human being.

4. To Practicing group learning through scientific inquiry.

PRACTICALS

1. Purification of an enzyme from any natural resource.

2. Quantitative estimation of proteins by Bradford method.

3. Effect of temperature on enzyme activity.

4. Effect of pH on enzyme activity.

5. Effect of substrate concentration on enzyme activity.

6. Calculation of kinetic parameters such as Km & Vmax.

Course Outcomes (COs)

PBBT-305 (I) CO 1.Generate and interpret the enzymes test hypotheses, analyze the data by

using modern methods

PBBT-305 (I) CO 2.Understand the designing of recent enzymological techniques and their

conducting experiments in laboratories

PBBT-305 (I) CO 3. Impart training on fundamentals of enzyme assay using different modern

tools and techniques.

PBBT-305 (I) CO 4.Demonstrate the enzyme isolation, purification, activity, kinetic parameters

such as Km and Vmax. References:

Books

1. Palmer & Bonner, Enzymes: Biochemistry, Biotechnology & Clinical Chemistry, Woodhead

Publishing.

Mode of Evaluation

External (MM-25)

Component End Semester Examination

Weightage 25

Page 78: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

CO-PO Matrix Enzymology Lab PBBT-305 (I)

Course Outcome PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PSO1 PSO2 PSO3 PSO4

PBBT-305 (I) CO 1. 1 2 - - - - - 2 - 1 -

PBBT-305 (I) CO 2. 1 2 - - - - - 2 - 1 -

PBBT-305 (I) CO 3. 1 2 - - - - - 3 - 2 2

PBBT-305 (I) CO 4. 1 2 - - - - - 3 - 3 2

Average CO (PBBT-

305 (I))

1 2 - - - - - 2.5 - 1.7 2

Page 79: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc (H.) Biotechnology Programme Code 05

Course Code TBBT-305 (II) Credit 3

Year/Sem 2/3 L-T-P 3-0-0

Course Name Drug Designing

Objectives of the Course:

1. Apply the basic knowledge about drug metabolic pathways, adverse effect and therapeutic

value of drugs

2. To understand how particular drugs were design or developed by using bioinformatics tools.

3. To develop the structural activity relationship of different class of drugs.

4. To explore the process of drug development, from target identification to final drug

registration.

UNIT I(Total Topics -10 and Hrs-7)

Introduction to Molecular Modelling: Introduction. Useful Concepts in Molecular Modelling:

Coordinate Systems. PotentialEnergy Surfaces. Molecular Graphics. Surfaces. Computer

Hardware and Software. The Molecular Modelling Literature.

UNIT II (Total Topics -11 and Hrs-7)

Force Fields:Fields. Bond Stretching. Angle Bending. Introduction to nonbonded interactions.

Electrostatic interactions. van der Waals Interactions. Hydrogen bonding in Molecular

Mechanics. Force Field Models for the Simulation of Liquid Water.

UNIT- III (Total Topics – 12 and Hrs-7)

Energy Minimization and Computer Simulation:Minimization and related methods for exploring

the energy surface. Non-derivative method,First and second order minimization methods.

Computer simulation methods. Simple thermodynamic properties and Phase Space. Boundaries.

Analyzing the results of a simulation and estimating Errors.

UNIT-IV (Total Topics –12 and Hrs-5)

Molecular Dynamics & Monte Carlo Simulation:Molecular Dynamics Simulation Methods.

Molecular Dynamics using simple models.Molecular Dynamics with continuous potentials.

Molecular Dynamics at constanttemperature and pressure. Metropolis method. Monte Carlo

simulation of molecules. Models used in Monte Carlo simulations of polymers.

UNIT-V (Total Topics -10and Hrs-10)

Structure Prediction and Drug Design:Structure prediction - Introduction to comparative

Modeling. Sequence alignment.Constructing and evaluating a comparative model. Predicting

protein structures by'Threading’, Molecular docking. Structure based de novo ligand design,

Drug Discovery – Chemoinformatics – QSAR

Course Outcomes (COs)

1. TBBT305(II) CO 1 To understand how current drugs were developed by using docking

technique and pharmacophores modeling.

2. TBBT305(II) CO 2 To utilize various molecular modeling softwares in the design of novel

drug-like molecules.

3. TBBT305(II) CO 3 To develop the structural activity relationship of different class of drugs.

4. TBBT305(II) CO 4 To explore the process of drug development, from target identification

to final drug registration.

Page 80: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

References:

Books

1. R. Leach, Molecular Modelling Principles and Application, Longman, 2001.

2. J.M. Haile, Molecular Dynamics Simulation Elementary Methods, John Wiley andSons,1997.

3. Satya Prakash Gupta, QSAR and Molecular Modeling, Springer – AnamayaPublishers, 2008.

Mode of Evaluation

Internal (MM-40) External (MM-60)

Component Sessional -1 Sessional -2 Assignments End Semester Examination

Weightage 10 10 20 60

CO-PO Matrix Drug Designing TBBT-305 (II)

Course Outcome PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PSO

1

PSO

2

PSO

3

PSO

4

TBBT 305 (II) CO1 - 3 2 1 1 1 2 2 2 2 2

TBBT 305 (II) CO2 2 3 2 2 - - 1 2 2 2 2

TBBT 305 (II) CO4 - - 2 - - - - - - - 1

TBBT 305 (II) CO4 2 1 2 2 2 1 - 2 2 2 2

Average CO TBBT-

305 (II) 2.0 2.3 2.0 1.6 1.5 1.0 1.5 2.0 2.0 2.0 1.7

Programme Name B.Sc (H.) Biotechnology Programme Code 05

Page 81: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Course Code PBBT 305 (II) Credit 1

Year/Sem 3/4 L-T-P 0-0-2

Course Name Drug Designing Lab

Objectives of the Course:

1. To demonstrate basic scientific knowledge about role of bioinformatics in drug discovery.

2. To perform different recent advanced tools and techniques to resolve drug designing

problems.

3. To learn about molecular recognition, computer aided drug design, and toxicology as applied

to the development of new medicines.

4. To develop the laboratory skills Safety evaluation, bioavailability, clinical trials, and the

essentials of patent laws.

UNIT I (Total Topics- 5 and Hrs-10)

1. 3D structure of protein

2. Visualization of protein structure using bioinformatics tools.

3. Demonstration of different softwares used in drug designing & modeling

Course Outcomes (COs)

PBBT-305(II) CO 1 To demonstrate studied on the various targets for drug discovery

PBBT-305(II) CO 2 Learnt the importance of the role of computer aided drug design in drug

discovery.

PBBT-305 (II) CO3 State the physicochemical properties of drug molecules.

PBBT-305(II) CO4To understand practical aspect of physicochemical properties of drugs

including solubility, distribution, adsorption, and stability

References:

Books

1. R. Leach, Molecular Modelling Principles and Application, Longman, 2001.

2. J.M. Haile, Molecular Dynamics Simulation Elementary Methods, John Wiley and Sons,

1997

Mode of Evaluation

External (MM-25)

Component End Semester Examination

Weightage 25

Page 82: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

CO-PO Matrix Drug designing Lab PBBT-305 (II)

Course Outcome PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7 PSO1 PSO2 PSO3 PSO4

PBBT-305 (II) CO1 2 2 2 2 3 1 1 3 2 2 -

PBBT-305 (II) CO2 2 - 2 1 - 2 1 2 - 2 -

PBBT-305 (II) CO3 1 - 1 1 1 - - - 2 - 1

PBBT-305 (II) CO4 - - 1 2 2 - - 2 2 1 2

Average CO (PBBT-

305 (II) 1.6 2.0 1.5 1.5 2.0 1.5 1.0 2.3 2.0 1.6 1.5

Page 83: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc (H)Biotechnology Programme Code 05

Course Code ADP-306 Credit 2

Year/Sem 2/3 L-T-P 0-0-4

Course Name Activity/ SWAYAM /NPTEL

Objectives of the Course:

1. To develop knowledge related to recent scientific research of applied science either through

assigned topic or online resources.

2. To enhance writing and presentation skills.

3. To enhance presentation and communication skill through participation in various activities. Academic activity

1. Each student will be allotted a relevant topic pertaining to recent developments in field of

biotechnology / life sciences or allied sciences.

2. Student will prepare a report on recent research conducted pertaining to the topic allotted.

3. Each student will present his / her report in form of power point presentation and a hardcopy

of same will be submitted to the department.

4. Evaluation will be done based upon presentation and submitted report.

SWAYAM/NPTEL

1. Students have to register online in any one course offered by NPTEL / SWAYAM /MOOC

courses and have to complete online course within prescribe time limit.

2. Evaluation of marks will be done on the basis of marks obtained by the student in that

particular course.

Course Outcomes (COs)

ADP-306 CO1. Develop knowledge related to recent scientific research of applied science either

through assigned topic or online resources.

ADP-306 CO2. Enhance writing and presentation skills.

ADP-306 CO3. Acquire critical thinking ability to analyze and interpret observations, recent

scientific developments, etc

Mode of Evaluation

External MM -50

Component Sessional

Weightage 50

Page 84: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

CO-PO Matrix Activity/ SWAYAM /NPTELADP-306

Course Outcome PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PSO

1

PSO

2

PSO

3

PSO

4

ADP-306CO 1. - - - - 1 - - 1 1 2 -

ADP-306CO 2. - - - - 3 - - - - -

ADP-306CO 3. - - - - 1 - - 1 - -

Average CO (ADP-

306) 1 1 - - 1 3 - 1 1 2 -

Page 85: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

SEMESTER- IV

Page 86: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc (H) Biotechnology Programme Code 05

Course Code TBBT-401 Credit 4

Year/Sem 2/4 L-T-P 4-0-0

Course Name Molecular Biology

Objectives of the Course:

1. To provide fundamental aspects of molecular biology with detailed basics of cellular

organization and biochemical molecules present in cell.

2. To study various important cellular mechanism like replication of DNA, transcription and

translation in prokaryotes and eukaryotes.

3. To provide basic understanding of DNA damage mechanism and its repair.

4. To learn genetic coding information and gene expression in prokaryotes and eukaryotes

UNIT I (Total Topics-10 and Hrs-10)

DNA replication: Replication of DNA in prokaryotes and eukaryotes: Semiconservative nature

of DNA replication, Bi-directional replication, DNA polymerases, Replication complex: Pre-

primming proteins, primosome, replisome; Rolling circle replication, Unique aspects of

eukaryotic chromosome replication, Fidelity of replication.

UNIT II (Total Topics-6 and Hrs-10)

DNA Damage, Repair and Homologous Recombination:DNA damage and repair: causes and

types of DNA damage, mechanism of DNA repair: Photoreactivation, base excision repair,

nucleotide excision repair, mismatch repair, recombinational repair. Homologous recombination:

models and mechanism

UNIT- III (Total Topics-10 and Hrs-10)

Transcription and RNA Processing: Transcription in prokaryotes: Prokaryotic RNA polymerase,

role of sigma factor, promoter, Initiation, elongation and termination of RNA chains;

Transcription in eukaryotes: Eukaryotic RNA polymerases, transcription factors, promoters,

enhancers, mechanism of transcription initiation, promoter clearance and elongation; RNA

splicing and processing: processing of pre-mRNA: 5’ cap formation, polyadenylation, splicing,

rRNA and tRNA splicing

UNIT-IV (Total Topics-10 and Hrs-10)

Translation & Regulation of Gene Expression : Genetic code and its characteristics; Prokaryotic

and eukaryotic translation: ribosome structure and assembly, Charging of tRNA, aminoacyl

tRNA synthetases, Mechanism of initiation, elongation and termination of polypeptides, Fidelity

of translation, Inhibitors of translation; Posttranslational modifications of proteins.

Regulation of gene expression in prokaryotes: Operon concept (inducible and repressible

system), Regulation of gene expression in eukaryotes

Course Outcomes (COs)

TBBT-401 CO1. Understand basic and advanced molecular biology concepts and techniques.

TBBT-401 CO2. Appraise domain-specific knowledge and develop globally-relevant skills for

academic and professional enhancement

TBBT-401 CO3. Identify underlying principle of working of various instrument and technique

used in molecular biology and application of same in scientific research.

TBBT-401 CO4. Demonstrate an understanding of molecular pathways that are altered in DNA

and various DNA repair and its mechanism

Page 87: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

References:

Books

1. Karp G., Cell and Molecular Biology, Concepts and Experiments, John Wiley & Sons,

2010,VI Edition.

2. Watson J. D., Baker T.A., Bell, S. P., Gann, A., Levine, M. and Losick, R., Molecular Biology

of the Gene ,Cold Spring Harbour Lab. Press, Pearson Pub, 2008 ,VI edition.

3. Brown T.A., Essential Molecular Biology, Oxford University Press.

4. Brown T.A., Essential Molecular Biology: A Practical Approach (vols I & II),Oxford

University Press.

Mode of Evaluation

Internal (MM-40) External (MM-60)

Component Sessional -1 Sessional -2 Assignments End Semester Examination

Weightage 10 10 20 60

CO-PO Matrix Molecular Biology TBBT-401

Course Outcome PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7 PSO1 PSO2 PSO3 PSO4

TBBT-401CO 1. 3 - 2 2 2 - - 3 3 3 -

TBBT-401CO 2. 2 - 1 2 2 - 2 2 2 2 -

TBBT-401CO 3. 2 - 2 1 1 - - 2 2 3 -

TBBT-401CO 4. 2 - - 1 1 - - 2 1 2 -

Average CO

(TBBT-401) 2.2 - 1.6 1.5 1.5 - 2 2.2 2 2.5 -

Page 88: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc (H) Biotechnology Programme Code 05

Course Code PBBT-401 Credit 2

Year/Sem 2/4 L-T-P 0-0-4

Course Name Molecular Biology Lab

Objectives of the Course:

1. To provide students basic knowledge of DNA isolation from various tissues using

molecularbiology techniques.

2. To understand, learn and apply the principle, tools and techniques of molecular biology,

whichprepares students for further study and/or higher education and employment.

3. To learn basic knowledge of protein profiling by SDS PAGE.

4. To learn basics of working and principle of DNA profiling using agarose gel electrophoresis.

PRACTICALS

1. Isolation of genomic DNA from blood sample.

2. Isolation of chromosomal DNA from bacterial cells.

3. Isolation of Plasmid DNA by alkaline lysis method.

4. Agarose gel electrophoresis of genomic DNA & plasmid DNA.

5. Isolation of protein from different sources.

6. Separation of protein using PAGE.

Course Outcomes (COs)

PBBT-401 CO1. Identify the basic molecular biology techniques which will enhance practical

knowledge, application of same in drug designing and in biological research.

PBBT-401 CO2. Identify the enzymes, organelles and molecules involved in replication,

transcription and translation and learn the role of each.

PBBT-401 CO3. Develops understanding of various molecular biology techniques, working and

principle of various instruments used in molecular biology and their respective application.

PBBT-401 CO4. Develop knowledge of interpretation of experimental data and evaluation of

experimental result to derive a solution to a problem. References:

Books

1. Wilson, K. and Walkers, J., Principles and Techniques of Biochemistry and Molecular

Biology, Cambridge University press.

Mode of Evaluation

Internal (MM-25) External (MM-25)

Component Internal End Semester Examination

Weightage 25 25

Page 89: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

CO-PO Matrix Molecular Biology Lab PBBT-401

Course Outcome PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PSO

1

PSO

2

PSO

3

PSO

4

PBBT-401CO 1. 2 - 2 2 2 - - 3 3 3 -

PBBT-401CO 2. 3 - 1 1 1 - - 2 1 2 -

PBBT-401CO 3. 2 - 3 2 2 - - 3 3 3 -

PBBT-401CO 4. 2 - 2 1 1 - - 2 2 1 -

Average CO (PBBT-

401) 2.2 - 2 1.5 1.5 - - 2.5 2.2 2.2 -

Page 90: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc (H) Biotechnology Programme Code 05

Course Code TBBT-402 Credit 4

Year/Sem 2/4 L-T-P 4-0-0

Course Name Immunology

Objectives of the Course:

1. To provide life-long learning to develop, implement & deal with its applications by studying

from basics to broad level of the vast immune systems organization in the body and its

various elements.

2. To avail students to learn the significance of the immune system to the genetic level & create

a broad area of understanding the genuine facts & make students more enthusiast to research

& build good career.

3. To provide the content knowledge about vaccination, immunodeficiency diseases etc and

how to implement modifications in a wise manner.

4. To avail critical thinking and help to applicate the knowledge to the various fields of allied

sciences to prevent certain diseases and find more precautionary majors as various

diagnostic techniques up rise as a pathfinder for the discovery and modifications in the

diagnostic fields.

UNIT I (Total Topics-12 and Hrs-10)

Immune Response - An overview, components of mammalian immune system – cells & organs,

molecular structure of Immunoglobulins or Antibodies & their different classes, Humoral &

Cellular immune responses, T-lymphocytes & immune response (cytotoxic T-cell, helper T-cell,

suppressor T-cells), T-cell receptors. Antigens & factors affecting antigenicity. Monoclonal

antibody: production, types & applications.

UNIT II (Total Topics-12 and Hrs-10)

Generation of Immunoglobulin Diversity – clonal selection theory, isotypes, allotypes &

idiotypes, allelic exclusion, immunologic memory, heavy chain gene transcription, genetic basis

of antibody diversity: antibody affinity maturation, somatic recombination, somatic mutation &

class switching. Complement system & its biological significance.

UNIT- III (Total Topics-6 and Hrs-10)

Major Histocompatibility complexes – class I & class II MHC antigens; antigen processing &

presentation. Immunity to infection – immunity to different organisms. Autoimmune diseases,

Immunodeficiency diseases.

UNIT-IV (Total Topics-10 and Hrs-10)

Vaccines & Vaccination – properties of ideal vaccines, adjuvants, cytokines, different

generations of vaccines. Immunization & its types. Introduction to immunodiagnostics –

Antigen-Antibody interaction: Agglutination, Precipitation, RIA, ELISA.

Course Outcomes (COs)

TBBT-402 CO1. Acquire knowledge about structural and functional organization of immune

system.

TBBT-402 CO2. Outline, compare and contrast the key mechanisms and cellular players of

innate and adaptive immunity and how they relate, principles of immunological methods and

data interpretation.

TBBT-402 CO3. Comprehend and appraise mechanisms of inflammation, vaccination, immune-

Page 91: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

deficiencies, complement system and allergic reactions and their application in health and

medicine.

TBBT-402 CO4. Explore and analyze applicability of immunological studies, tools and

techniques in disease diagnosis and addressing health related issues References:

Books

1. Abbas, A.K., Lichtman, A.H., and Pillai, S., Cellular and Molecular Immunology., Saunders

Publication, Philadelphia, 2007, 6th edition

2. Delves, P., Martin, S., Burton, D. and Roitt, I.M., Roitt’s Essential Immunology., Wiley-

Blackwell Scientific Publication, Oxford, 2006, 11th edition

3. Goldsby R.A., Kindt, T.J. and Osborne, B.A. Kuby’s, Immunology,W.H. Freeman and

Company New York.,2007, 6th edition.

4. Khan, F.H., Elements of Immunology.

5. Lydyard P., BIOS, Instant Notes Immunology.

CO-PO Matrix Immunology TBBT-402

Course Outcome PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PSO

1

PSO

2

PSO

3

PSO

4

TBBT-402 CO 1. 2 - - - - - - 2 - - 1

TBBT-402 CO 2. - 2 2 2 - - - 1 2 1 -

TBBT-402CO 3. - - 1 - - - - - - 2 -

TBBT-402CO 4. - 2 2 - 2 - - 2 2 - 1

Average CO

(TBBT-402) 2 2 1.6 2 2 - - 1.6 2 1.5 1

Mode of Evaluation

Internal (MM-40) External (MM-60)

Component Sessional -1 Sessional -2 Assignments End Semester Examination

Weightage 10 10 20 60

Page 92: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc (H) Biotechnology Programme Code 05

Course Code PBBT-402 Credit 2

Year/Sem 2/4 L-T-P 0-0-4

Course Name Immunology Lab

Objectives of the Course:

1. To implement&deal with the practical aspects in the laboratory by performing various

immunological tests for medical interventions.

2. To State the method and use of specimen collection and handling requirements for each

immunological tests and safety procedures.

3. To Discuss the theory and principles of operation of the assays such as haemagglutination

,ELISAetc with a detailed understanding of antigen antibody reactions.

4. To applicate the laboratory skills to the various fields of allied sciences and researches.

PRACTICALS

1.Single radial immunodiffusion

2. Double immunodiffusion

3. Haemagglutination assay; Haemagglutination inhibition assay

4. Demonstration / study of Immunoelectrophoresis

5. Sandwich ELISA

6. Competitive ELISA

7. Dot-ELISA

Course Outcomes (COs)

PBBT-402 CO1. Exhibit analytical skills to conduct laboratory diagnosis of infectious diseases

( ELISA, RIA ) and identify their application in medicine.

PBBT-402 CO2. Understand the link of Immunology to other disciplines of health and

experimental biosciences with the acknowledgment to the practical part of theoretical studies.

PBBT-402 CO3. Acquiring the ability to integrate experimental design, data analysis,

appreciation of the scientific method, refining the career &educational opportunities in various

disciplines of science.

PBBT -402 CO4. Demonstrate ability to conduct qualitative & quantitative immunological

estimations and analysis along with data analysis to derive a conclusion.

References:

Books

1.Hay Frank C Olwyn M. R. Westwood, Practical Immunology, Wiley-Blackwell ,15 February

2000,4th edition

Mode of Evaluation

Internal (MM-25) External (MM-25)

Component Internal End Semester Examination

Weightage 25 25

Page 93: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

CO-PO Matrix Immunology Lab PBBT -402

Course Outcome PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PSO

1

PSO

2

PSO

3

PSO

4

PBBT -402 CO 1. 2 - - - - - - 2 - 3 1

PBBT -402 CO 2. - 2 - 1 - - 1 - - 2 1

PBBT -402CO 3. 1 1 2 - - - 2 - - 3 -

PBBT -402CO 4. - - 2 - 2 - - - 2 - -

Average CO

(PBBT -402) 1.5 1.5 2 1 2 - 1.5 2 2 2.6 2

Page 94: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc (H) Biotechnology Programme Code 05

Course Code TBBT-403 Credit 4

Year/Sem 2/4 L-T-P 4-0-0

Course Name Chemistry II

Objectives of the Course:

1. To provide the basic knowledge of all the physical processes which are very useful in our

daily practice.

2. To acquire the knowledge of phase rule, electrochemical cell as well as properties of

solution.

3. To make students able to find out the effect of temperature, pressure and concentration on

any chemical reaction by applying Le- Chaterliers principles.

4. To identify the type of reaction either exothermic or endothermic, also able to explain the

feasibility of reaction.

5. To help students in preparation of higher competition exams.

UNIT I (Total Topics-10 and Hrs-10)

Chemical Energetics: Laws of Thermodynamics. I and II law of Thermodynamics, Hess law.

Concept of standard state and standard enthalpies of formations, integral and differential

enthalpies of solution. Calculation of bond energy, bond dissociation energy and resonance

energy from thermochemical data. Variation of enthalpy of a reaction with temperature –

Kirchhoff’s equation. Statement of Third Law of thermodynamics and calculation of absolute

entropies of substances.

UNIT II(Total Topics-11 and Hrs-10)

Chemical & Ionic Equilibrium: Chemical Equilibrium. Le Chatelier’s principle. Relationships

between Kp& Kc for reactions involving ideal gases.Strong, moderate and weak electrolytes,

degree of ionization, factors affecting degree of ionization, ionization constant and ionic product

of water. Ionization of weak acids and bases, pH scale, common ion effect. Salt hydrolysis-

calculation of hydrolysis constant, degree of hydrolysis and pH for different salts.. Solubility and

solubility product of salts and applications.

UNIT- III(Total Topics-10 and Hrs-10)

Solution: Introduction & non ideal solution, ideal solutions: Ideal solutions and Raoult’s law,

non-ideal solutions. Vapour pressure-composition and temperature composition curves of ideal

and non-ideal solutions. Distillation of solutions. Lever rule. Azeotropes. Partial miscibility of

liquids: Critical solution temperature; effect of impurity on partial miscibility of liquids.

Immiscibility of liquids- Principle of steam distillation. Nernst distribution law and its

applications, solvent extraction.

UNIT-IV (Total Topics-8 and Hrs-10)

Phase Equilibrium:Phases, components and degrees of freedom of a system, criteria of phase

equilibrium. Gibbs Phase Rule. Clausius – Clapeyron equation and its importance in phase

equilibria. Phase diagrams of one-component systems (water and sulphur) and two component

systems involving eutectics, congruent and incongruent melting points (lead-silver & FeCl3-

H2O only).

UNIT V(Total Topics-10 and Hrs-10)

Page 95: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Electrochemistry: Conductance, Conductivity, equivalent and molar conductivity and their

variation with dilution for weak and strong electrolytes. Kohlrausch law .Reversible and

irreversible cells. Concept of EMF of a cell. Measurement of EMF of a cell. Nernst equation and

its importance. Types of electrodes. Standard electrode potential. Electrochemical series and

application. Thermodynamics of a reversible cell, calculation of thermodynamic properties: ΔG,

ΔH and ΔS from EMF data. Calculation of equilibrium constant from EMF data.

Course Outcomes (COs)

TBBT-403 CO 1.Understand the thermodynamics terminology, types of reaction process,

feasibility of reaction and concepts of heat, work or mass in thermodynamics.

TBBT-403 CO 2. Understanding of electrochemical equipments, application of electrochemical

series and reactivity of metals.Also acquire knowledge about electrolytes, pH of solutions,

concept of solubility and buffers which is highly applicable to identify the chemical nature of

solution.

TBBT-403 CO 3. Understand basics of ideal and non-ideal solution, Raoults law application and

idea of vapour pressure of solution.

TBBT-403 CO 4. Design a system component or aqueous chemical process involving ideal and

non ideal system. References:

Books

1. Atkeins, P.W. and Paula, J.de. Atkins., physical chemistry, Ed. Oxford University, 2006

2. Bahl and Tuli, physical chemistry, S. chand publication,2009

Mode of Evaluation

Internal (MM-40) External (MM-60)

Component Sessional -1 Sessional -2 Assignments End Semester Examination

Weightage 10 10 20 60

CO-PO Matrix Chemistry II TBBT-403

Course Outcome PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PSO

1

PSO

2

PSO

3

PSO

4

TBBT-403 CO 1. - 2 1 - - - 1 2 - - 1

TBBT-403 CO 2. - 2 1 - - - 1 2 - - 1

TBBT-403 CO 3. - 2 1 - - - 1 2 - - 1

TBBT-403 CO 4. - 2 1 - - - 1 2 - - 1

Average CO(TBBT -

403) 1.5 1.5 2 1 2 - 1.5 2 2 2.6 2

Page 96: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc (H) Biotechnology Programme Code 05

Course Code PBBT-403 Credit 1

Year/Sem 2/4 L-T-P 0-0-2

Course Name Chemistry II Lab

Objectives of the Course:

1.To practically apply the concept of estimation of metal as well as compounds fromgiven

solution also analyse the role of indicators.

2. To develop experimental skills of detection of elements from given mixture.

3. Recognize various functional groups and aromaticity in compounds.

4. Set up glassware and apparatus to conduct experiments in chemistry laboratory.

PRACTICALS

1. Construction of the phase diagram of a simple system

2. Study of phase diagram one component and two companant system (simple eutectic)

using cooling curves.

3. Determination of the critical solution temperature and composition of the phenol

a. Water system and study of the effect of impurities on it.

4. Study of the variation of mutual solubility temperature with concentration for the

a. Phenol water system and determination of the critical solubility temperature.

5.Determination of equivalent conductance, degree of dissociation and dissociation constant of

a weak acid.

6. Study of conductmetric titrations: a. Strong acid vs. strong base b. Weak acid vs. strong base

7. Preparation of different buffer solutions

Determination of equivalent conductance, degree of dissociation and dissociation constant of a weak acid. 6. Study of conductometric titrations: a. Strong acid vs. strong base b. Weak acid vs. strong base 7. Preparation of different buffer solutions

b. Determination of cell constant

5. Determination of equivalent conductance, degree of dissociation and dissociation

constant of a weak acid.

6. Study of conductometric titrations:

a. Strong acid vs. strong base

b. Weak acid vs. strong base

7. Preparation of different buffer solutions.

Course Outcomes (COs)

PBBT-403 CO1. Facilitate the learner to make solutions of various concentrations.

PBBT-403 CO2. Apply knowledge to identify the nature of organic compounds and learn

chemical formula of inorganic salts.

PBBT-403 CO3. Ability to perform and estimate the strength of given ion or compounds by

titrations.

PBBT-403 CO4. After performing and understanding the laboratory experiments students can

apply for employments in field of analysis techniques. References:

Books

1. Vogel’s Qualitative Inorganic Analysis, A.I. Vogel, Prentice Hall, 7 th Edition.

2. Vogel’s Quantitative Chemical Analysis, A.I. Vogel, Prentice Hall, 6 th Edition.

3. Textbook of Practical Organic Chemistry, A.I. Vogel, Prentice Hall, 5 th Edition.

4. Practical Organic Chemistry, F. G. Mann. & B. C. Saunders, Orient Longman

Page 97: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Mode of Evaluation

Internal (MM-25) External (MM-25)

Component Internal End Semester Examination

Weightage 25

25

CO-PO Matrix Chemistry II Lab PBBT-403

Course Outcome PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PSO

1

PSO

2

PSO

3

PSO

4

PBBT-403 CO 1. - 2 1 - - - 1 1 2 - -

PBBT-403 CO 2. - 1 1 - - - 1 1 - - 1

PBBT-403 CO 3. - 1 1 - - - 1 2 1 - -

PBBT-403 CO 4. - 1 1 - - - 1 1 1 - 1

Average CO(PBBT-

403) - 1.2 1 - - - 1 1.25 1.33 - 1

Page 98: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc (H) Biotechnology Programme Code 05

Course Code TBBT-404 Credit 3

Year/Sem 2/4 L-T-P 3-0-0

Course Name Entrepreneurship Development

Objectives of the Course:

1. To understand the basic of Entrepreneurship for development of entrepreneurship goals

Business management to work in team.

2. To provide knowledge regarding application of business management and its impact in

society and environment

3. To explore knowledge regarding financing of enterprises, marketing management and

selection of products & markets in National and International business.

4. To provide management skills for establishment of small scale business.

UNIT I (Total Topics-5 and Hrs-5)

Introduction: Meaning, Needs and Importance of Entrepreneurship, Promotion of

entrepreneurship, Factors influencing entrepreneurship, Features of a successful

Entrepreneurship.

UNIT II(Total Topics-5 and Hrs-5)

Establishing an enterprise: Forms of Business Organization, Project Identification, Selection of

the product, Project formulation, Assessment of project feasibility.

UNIT- III (Total Topics-8 and Hrs-10)

Financing the Enterprise

Importance of finance / loans and repayments, Characteristics of Business finance, Fixed capital

management: Sources of fixed capital, working capital its sources and how to move for loans,

Inventory direct and indirect raw materials and its management.

UNIT-IV (Total Topics-9 and Hrs-10)

Marketing Management: Meaning and Importance, Marketing-mix, product management –

Product line, Product mix, stages of product like cycle, marketing Research and Importance of

survey, Physical Distribution and Stock Management.

UNIT V(Total Topics-5 and Hrs-5)

Entrepreneurship and International Business: Meaning of International business, Selection of a

product, Selection of a market for international business, Export financing, Institutional support

for exports

Course Outcomes (COs)

TBBT-404 CO1. Identify and understand the basic of Entrepreneurship and Business

management along with imparting ability to work in team

TBBT-404 CO2. Provide sound knowledge pertaining to application of business strategies and

impact of same to society and environment

TBBT-404 CO3. Explore knowledge regarding entrepreneurship developments for selection of

products and markets in National and International business.

TBBT-404 CO4. Appraise and develop management skills as life learning process for designing

and development of startups or scientific project.

References:

Books

Page 99: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

1. Holt, D.H., Entrepreneurship: New Venture Creation, PHI, 1998.

2. Kaplan, J.M., Patterns of Entrepreneurship, Wiley, 2016.

3. Gupta, C.B. and Khanka, S.S., Entrepreneurship and Small Business Management, Sultan

Chand & Sons.

Mode of Evaluation

Internal (MM-40) External (MM-60)

Component Sessional -1 Sessional -2 Assignments End Semester Examination

Weightage 10 10 20 60

CO-PO Matrix Entrepreneurship Development TBBT-404

Course Outcome PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PSO

1

PSO

2

PSO

3

PSO

4

TBBT-404 CO 1. 2 1 3 3 1 3

TBBT-404 CO 2. 2 1 2 2 1 1 1

TBBT-404 CO 3. 2 1 3 1 1 2

TBBT-404 CO 4. 1 3 2 2

Average CO(TBBT-

404) 1.7 1 2.7 2 1 1 1 2

Page 100: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc (H) Biotechnology Programme Code 05

Course Code PBBT-404 Credit 1

Year/Sem 2/4 L-T-P 0-0-2

Course Name Entrepreneurship Development Lab

Objectives of the Course:

1. To understand the procedure for development of entrepreneurship goals Business

management to workin team.

2. To provide knowledge capital management and its optimization in business development.

3. To explore knowledge and prerequisites for financing of enterprises, marketing management

andselection of productsmarkets in National and International business.

Course Outcomes (COs)

PBBT-404 CO1. Acquire knowledge to indent the need, importance, application and process of

Entrepreneurship development.

PBBT-404 CO2. Demonstration of skills related to safety guidelines, laboratory layout and chart

preparation for four levels of biosafety labs.

PBBT-404 CO3. Development of knowledge related to prerequisites for Enterprise

establishment, capital management and essential modulus of business report for the development

of Entrepreneurships.

PBBT-404 CO4. Appraise and develop management skills for optimizing capital management

and Regulatory Organizations for setting up a Scientific Enterprise unit.

References:

Books

1. Holt D.H., Entrepreneurship: New Venture Creation, PHI, 1998.

2. Kaplan, J.M., Patterns of Entrepreneurship, Wiley, 2016.

3. Gupta, C.B. and Khanka, S.S., Entrepreneurship and Small Business Management, Sultan

Chand & Sons.

Mode of Evaluation

External MM -25

Component Sessional

Weightage 25

CO-PO Matrix Entrepreneurship Development Lab PBBT-404

Course Outcome PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PSO

1

PSO

2

PSO

3

PSO

4

PBBT-404 CO 1. 2 - 1 3 - - 2 - 1 - 2

PBBT-404 CO 2. 2 - 1 2 - - 1 1 - 1 -

PBBT-404 CO 3. 2 - 2 3 - - 1 - - 1 1

PBBT-404 CO 4. 1 - 1 3 - - 1 - - - 1

Average CO(PBBT-

404) 1.7 - 1.2 2.7 - - 1.2 1 1 1 1.3

Page 101: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc. H Biotechnology Programme Code 05

Course Code TBBT-405 (I) Credit 3

Year/Sem 2/4 L-T-P 3-0-0

Course Name Molecular diagnostics

Objectives of the Course:

1. Students will understand the fundamental aspects of diagnostics assays for biomarkers,

enzyme assays and antibody based detection for advanced healthcare system.

2. To choose appropriate laboratory exercises are designed to provide a hands-on context for

the topics being presented in the course lectures and in the readings from the course textbook.

3. To apply the knowledge of molecular testing to the most commonly performed applications

in the clinical laboratory such as: nucleic acid extraction, resolution and detection, analysis

and characterization of nucleic acids.

4. To assess design and practical skills such as use of molecular techniques in the diagnostics

labs, academic, higher research and clinical industries.

UNIT I (Total Topics-10 and Hrs-10)

Introduction to Molecular Diagnostics: History of diagnostics; age of molecular diagnostics,

significance and scope. Rise of diagnostic industry in Indian and global scenario. Diagnostic

pathology (bacterial, viral, fungal and protozoan), immune pathology, and

immunohistopathology. Drug susceptibility testing, cellular and functional genomics in

diagnostics

UNIT II (Total Topics-9 and Hrs-10)

Enzyme Immunoassays: Comparison of enzymes available for enzyme immunoassays,

conjugation of enzymes. Solid phases used in enzyme immunoassays. Homogeneous and

heterogeneous enzyme immunoassays. Enzyme immunoassays after immuno blotting. Enzyme

immuno histochemical techniques. Use of polyclonal or monoclonal antibodies in enzymes

immuno assays.

UNIT- III (Total Topics-13 and Hrs-5)

Molecular diagnostic methods: PCR, RFLP, Nuclear hybridization methods, ELISA, Western

Blotting, Single nucleotide polymorphism detection, DNA foot printing, Diagnosis of Genetic

Disorders. Immunoelectrophoresis, Dot-Blot, Rocket Electrophoresis, Ouchterlony and WIDAL.

UNIT-IV (Total Topics-8 and Hrs-5)

Diagnostic techniques: GLC, HPLC, Electron microscopy, flowcytometry and cell sorting,

Imaging of cancer cells, Applications of Nanotechnology in Detection and diagnostics. Detection

of Transgene in Transgenic animals and plants.

Course Outcomes (COs)

TBBT-405 (I) CO1Inculcate ability to design and assess theoretical skills such as the use of

molecular techniques in the diagnostics labs, academic, higher research and clinical industries

TBBT-405 (I) CO2Fundamental aspects of Immunodiagnostics and assays for biomarkers,

enzyme assays, and antibody-based detection.

TBBT-405 (I) CO3Provide basic and advanced diagnostic techniques that explore research and

training in higher education and applicable to the modern medicinal treatment system.

TBBT-405 (I) CO4Apply the knowledge of molecular testing to the most commonly performed

applications in the clinical laboratory such as nucleic acid extraction, resolution and detection,

Page 102: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

analysis and characterization of nucleic acids and proteins, nucleic acid amplification and DNA

sequencing for contribution in modern science

References:

Books

1. Burns, D. E., Fundamentals of Molecular Diagnostics.

2. Singh, B. D., Expanding horizons biotechnology, Kalyani publication.

Mode of Evaluation

Internal (MM-40) External (MM-60)

Component Sessional -1 Sessional -2 Assignments End Semester Examination

Weightage 10 10 20 60

CO-PO Matrix Molecular diagnostics TBBT-405 (I)

Course Outcome PO1 PO2 PO3 PO4 PO5 PO6 PO7 PSO1 PSO2 PSO3 PSO4

TBBT-405 (I) CO 1 3 2 - 2 1 2 2 2 2 2 -

TBBT-405 (I) CO 2 2 - - - - - - - 1 - -

TBBT-405 (I) CO 3 1 - 3 - 2 1 - - 1 2 2

TBBT-405 (I) CO 4 2 - - 1 - - - - 1 - -

Average CO(TBBT-405 (I) 2.0 2.0 3.0 1.5 1.5 1.5 2.0 2.0 1.2 2.0 2.0

Page 103: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc. H Biotechnology Programme Code 05

Course Code PBBT-405 (I) Credit 1

Year/Sem 2/4 L-T-P 0-0-2

Course Name Molecular Diagnostics Lab

Objectives of the Course:

1. To understand concept, principle of molecular diagnostic techniques along with data

analysis.

2. Interpret the test hypotheses; analyze the data of molecular diagnostic by using modern

instrumentation methods

3. To learn the practical techniques of antibiotic sensitive test and comprehend application the

same.

4. To comprehend the principle of the diagnostic test and their utility in disease diagnosis.

PRACTICALS

1.Perform/demonstrate RFLP and its analysis

2. Study of antibiotic sensitivity of a bacterialculture

3.Kit-based detection of a microbial infection (Widal test)

4. Study of Electron micrographs (any four).

5. Perform any one immuno diagnostic test ( Typhoid, Malaria, Dengue)

Course Outcomes (COs)

PBBT-405 (I) CO1. Ability to utilize RFLP techniques as diagnostic tool alongwith data

analysis.

PBBT-405 (I) CO2. Exhibit technical skills to perform antibiotic sensitivity test and data

interpretation.

PBBT-405 (I) CO3. Identify underlying concept of diagnostic techniques and applicability in

various disease.

PBBT-405 (I) CO4. Demonstrate ability to analyze interpret electron microscopy to derive the

conclusion

References:

Books

1. Burns, D. E., Fundamentals of Molecular Diagnostics.

2. Singh, B. D., Expanding horizons biotechnology, Kalyani publication.

Mode of Evaluation

External MM -25

Component Sessional

Weightage 25

Page 104: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

CO-PO Matrix Molecular diagnosticsLab PBBT-405 (I)

Course Outcome PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PSO

1

PSO

2

PSO

3

PSO

4

PBBT-405 (I) CO 1. 2 2 - 2 1 2 2 2 2 2 -

PBBT-405 (I) CO 2. 2 - - - - - - - 2 - -

PBBT-405 (I) CO 3. 1 - 3 - 2 2 - - 2 2 2

PBBT-405 (I) CO 4. 2 - - 1 - - - - 3 1 -

Average CO(PBBT-405

(I)) 1.7 2.0 3.0 1.5 1.5 2.0 2.0 2.0 2.2 1.6 2.0

Page 105: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc (H.) Biotechnology Programme Code 05

Course Code TBBT 405 (II) Credit 3

Year/Sem 2/4 L-T-P 3-0-0

Course Name Basic of Forensic Science

Objectives of the Course:

1. Apply the basic and advanced tools and techniques of forensic biology for employment,

higher studies, and advanced research to solve legal issues.

2. To develop knowledge about specific criminal Law, Indian Penal Code: sections, Criminal

Procedure Code and police Administration.

3. To design and develop the research skills associate with principle, function and different

branches of forensic biology.

4. Develop an appreciation of scientific and social environment of the criminal justice system.

UNIT I(Total Topics -10 and Hrs-7)

Introduction and principles of forensic science, forensic science laboratory and its organization

and service, tools and techniques in forensic science, branches of forensic science, causes of

crime, role of modus operandi in criminal investigation. Classification of injuries and their

medico-legal aspects, method of assessing various types of deaths.

UNIT II (Total Topics -11 and Hrs-7)

Classification of fire arms and explosives, introduction to internal, external and terminal

ballistics. Chemical evidence for explosives. General and individual characteristics of

handwriting, examination and comparison of handwritings and analysis of ink various samples.

UNIT- III (Total Topics – 12 and Hrs-7)

Role of the toxicologist, significance of toxicological findings, Fundamental principles of

fingerprinting, classification of fingerprints, development of finger print as science for personal

identification.

UNIT-IV (Total Topics –12 and Hrs-5)

Principle of DNA fingerprinting, application of DNA profiling in forensic medicine,

Investigation Tools, eDiscovery, Evidence Preservation, Search and Seizure of Computers,

Introduction to Cyber security.

Course Outcomes (COs)

TBBT405 (II) CO 1 Demonstrate basic scientific knowledge and understanding of forensic

science.

TBBT 405 (II) CO 2 Develop an appreciation of scientific and social environment of the

criminal justice system.

TBBT 405 (II) CO 3 Perform different recent advanced forensic tools and techniques.

TBBT 405 (II) CO 4Summaries about use of scientific evidence in a legal context.

References:

Books

1. Nanda B.B. and Tiwari, R. K. Forensic Science in India: A Vision for the Twenty First

Century, Select Publishers, New Delhi 2001

2. Bhasin M.K. and Nath, S. Role of Forensic Science in the New Millennium, University of

Delhi, Delhi 2002

Page 106: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

3. James S. H. and NordJ. J. Forensic Science: An Introduction to Scientific and Investigative

Techniques, CRC Press, Boca Raton 20052ND Edition.

Mode of Evaluation

Internal (MM-40) External (MM-60)

Component Sessional -1 Sessional -2 Assignments End Semester Examination

Weightage 10 10 20 60

CO-PO Matrix Basic of forensic science TBBT 405 (II)

Course Outcome PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7 PSO1 PSO2 PSO3 PSO4

TBBT 405 (II) CO1 3 3 2 1 1 1 2 2 2 2 2

TBBT 405 (II) CO2 2 3 2 2 - - 1 2 2 2 2

TBBT 405 (II) CO3 1 1 2 - 1 1 1 - 1 2 1

TBBT 405 (II) CO4 2 1 2 2 2 1 - 2 2 2 2

Average CO TBBT

405 (II) 2.0 2.0 2.0 1.2 1.3 1.0 1.3 1.50 1.75 2.00 1.75

Page 107: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc (H.) Biotechnology Programme Code 05

Course Code PBBT 405 (II) Credit 1

Year/Sem 2/4 L-T-P 0-0-2

Course Name Basic of forensic science lab

Objectives of the Course:

1. Documentation of crime scene by photography, sketching and field notes.

2.Case studies to depict different types of injuries and death.

3.Separation of nitro compounds (explosives)/ ink samples by thin layer chromatography.

4.Investigate method for developing fingerprints by Iodine crystals.

5.PCR amplification on target DNA and DNA profiling,

UNIT I (Total Topics- 5 and Hrs-10)

1. Documentation of crime scene by photography, sketching and field notes.

2.Case studies to depict different types of injuries and death.

3.Separation of nitro compounds (explosives)/ ink samples by thin layer chromatography.

4.Investigate method for developing fingerprints by Iodine crystals.

5.PCR amplification on target DNA and DNA profiling,

Course Outcomes (COs)

PBBT 405 (II) CO1 Interpret the application of DNA fingerprinting in forensic science.

PBBT 405 (II) CO2 Demonstrate crime scene investigation by important case study.

PBBT 405 (II) CO3 Learn the police science role in criminal investigation and Prevention of

crime

PBBT 405(II) CO4Explain the tools for faithful preservation of data on disks for analysis

References:

Books

1. Nanda B. B. and Tiwari, R .K. Forensic Science in India: A Vision for the Twenty First

Century, Select Publishers, New Delhi 2001

2. Bhasin M. K. and Nath, S. Role of Forensic Science in the New Millennium, University of

Delhi, Delhi 2002

Mode of Evaluation

External MM -25

Component Sessional

Weightage 25

Page 108: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

CO-PO Matrix Basic of Forensic Science Lab PBBT-405 (II)

Course Outcome PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PSO

1

PSO

2

PSO

3

PSO

4

PBBT-405 (II) CO1 2 2 2 2 - - 2 2 2 2

PBBT-405 (II) CO2 1 2 1 2 1 2 1 2 2 2 2

PBBT-405 (II) CO3 2 2 - - 1 1 2 - 3 - 2

PBBT-405 (II) CO4 - - 2 2 - 1 - 3 - - 1

Average CO PBBT-

405 (II) 1.6 2.0 1.6 2.0 1.3 1.3 1.5 2.33 2.33 2.00 1.75

Page 109: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc. (H ) Biotechnology Programme Code 05

Course Code ADP-406 Credit 1

Year/Sem 2/4 L-T-P 0-0-2

Course Name Seminar Presentation

Objectives of the Course:

1. To demonstrate technical skills for effective preparation of presentations, write-ups through

participant in academic and extracurricular activities.

2. To exhibit good communication and presentation skills.

3. To acquire critical thinking ability to analyze and interpret observations, recent scientific

developments, etc.

Guidelines:

• A topic of seminar related to biotechnology filed will be assigned to all students by the

faculty member.

• Students have to give a presentation in the form of PPT in seminar hall submit a hard copy.

• Evaluation will be done on the basis of presentation and submission of report on assigned

topic to the faculty.

Course Outcomes (COs)

ADP-406 CO1. Acquaintance about recent development and current trends in Biotechnology

and allied sciences.

ADP-406 CO2. Expansion of communication and presentation skills by substantive participation

in research group meetings, oral and poster presentations.

ADP-406 CO3. Acquire skills to utilize various techniques such as power point template,

Microsoft excel, paint, for preparation and compilation of effective presentation.

Mode of Evaluation

Internal (MM-50) External (MM-50)

Component Internal End Semester Examination

Weightage 50 50

Course

Outcome

PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7 PSO1 PSO2 PSO3 PSO4

ADP-406CO 1. - - - - - 3 - - - - -

ADP-406CO 2. - - - - - 3 - - - - -

ADP-406CO 3. - - - - 1 3 - - - 1 -

Average

CO(ADP-406) - - - - 1 3 - - - - -

Page 110: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc. (H) Biotechnology Programme Code 05

Course Code ADP-407 Credit 0

Year/Sem 2/4 L-T-P 2-0-0

Course Name Personality Development Program

Objectives of the Course:

1. To check if candidates have the adequate problem solving and analytical skills needed in an

organization. The person should have enough problem solving skills to listen to the problem

and immediately identify the proper solution.

2. To gamut the skills which facilitate them to enhance their employability quotient and overall

personality development

3. To understand these skills and enable students to manage the placement challenges more

effectively.

UNIT I (Total Topics- 2 and Hrs- 5)

Series Completion (Basic Concepts and Problems Solving According to Difficulty

Level)Number Series, Alphabet Series, Alpha-Numeric Series; Coding Decoding (Basic

Concepts and Problems Solving According to Difficulty Level) Letter Coding, Direct Letter

Coding, Number/Symbol Coding, Matrix Coding, Substitution, Deciphering Message

Word Code\s, Deciphering Number and Symbol Codes for Messages

UNIT II (Total Topics -3 and Hrs-5)

Blood Relations (Basic Concepts and Problems Solving According To Difficulty Level)

Deciphering Jumbled Up Descriptions, Relation Puzzles, Coding Relations.Direction Sense Test

(Basic Concepts and Problems Solving According To Difficulty Level) Problems based on

Distance and Direction, Problems based on Angles,Alphabet Test (Basic Concepts and Problems

Solving According To Difficulty Level) Alphabetical Quibble, Letter Word Problems, Rule

Detection.

UNIT- III (Total Topics -5 and Hrs-9)

Alpha Numeric Sequence Puzzles (Basic Concepts and Problems Solving According To

Difficulty Level)Number, Ranking and Time Sequence Test (Basic Concepts and Problems

Solving According To Difficulty),Problems Based on Seating/Standing Positions, Problems

based on Numbers, Problems based on Days ,Venn Diagram(Basic Concepts and Problems

Solving According To Difficulty Level),Syllogism (Basic Concepts and Problems Solving

According To Difficulty Level),Inequalities (Basic Concepts and Problems Solving According

To Difficulty Level):Direct Inequalities, Coded Inequalities.

UNIT-IV (Total Topics -4 and Hrs-10)

Seating Arrangement (Basic Concepts and Problems Solving According To Difficulty

Level),Circular Arrangement, Linear Arrangement (Facing North and Facing South),

Rectangular Arrangement, Mix Seating Arrangement (Blood Relation with Seating

Arrangement) , Analytical Reasoning (Puzzle Test) (Basic Concepts and Problems Solving

According To Difficulty Level),Classification Type Questions, Comparison Type

Question,Sequential Order Of Things, Selection Based On Given Conditions, Family Based

Puzzles,Analogy and Classification (Basic Concepts and Problems Solving According To

Difficulty Level),Problems based on Numbers, Problems based on Alphabets,Data

Sufficiency(Basic Concepts and Problems Solving According To Difficulty Level),Data

Page 111: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Sufficiency related to Seating Arrangement, Direction Sense, Blood Relation, Coding Decoding

etc.

Course Outcomes (COs)

ADP-407 CO1. Understand the soft skills and effective professional communication

ADP-407 CO2. Interview Skills

ADP-407 CO3. Industrial Etiquette

ADP-407 CO4. Professional grooming

ADP-407 CO5. Written English Skills including Presentation and Interview skills.

ADP-407 CO6. Oral Skills, pronunciation, accent, telephonic conversation and listening skill.

Reference Books: 1. Aggarwal R.S ,Quantitative Aptitude ,S. Chand Publisher,2017 2. Aggarwal R.S ,A Modern Approach to Logical Reasoning ,S. Chand Publisher,2017 3. Aggarwal R.S ,A Modern Approach to Verbal & Non-Verbal Reasoning , S. Chand

Publishe,2017 4. Sharma Arun,Quantitative Aptitude , MHE Publisher,2018,8th Edition

Mode of Evaluation

Internal MM -50

Component Sessional

Weightage 50

CO-PO Matrix Personality Development Program ADP-407

Course Outcome PO1 PO2 PO3 PO4 PO5 PO6 PO7 PSO1 PSO2 PSO3 PSO4

ADP-407 CO 1. - - - - - 2 - - - - -

ADP-407 CO 2. - - - - - - - - - - -

ADP-407 CO 3. - - - - - - - - - - -

ADP-407 CO 4. - - - - - - - - - - -

ADP-407 CO 5. - - - - - 3 - - 2 - -

ADP-407 CO 6. - - - - - 1 - - 1 - -

Average CO(ADP-

407) - - - - - 2 - - 2.5 - -

Page 112: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

SEMESTER- V

Page 113: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc. (H) Biotechnology Programme Code 05

Course Code TBBT-501 Credit 4

Year/Sem 3/5 L-T-P 4-0-0

Course Name Bio-process Technology

Objectives of the Course:

1. To impart basic knowledge of experimental techniques associated with aseptic process,

media preparation and related upstream and downstream process.

2. To provide the knowledge related to needs of various parts of fermenter and their operation

in laboratory as well as industrial level.

3. To understand conditions that influence growth and fermentation in industry and research.

4. To gain the technical skills for scale-up in fermentation process from laboratory scale to

industrial scale.

UNIT I (Total Topics- 7 and Hrs- 10)

Introduction to bioprocess technology. Range of bioprocess technology and its chronological

development. Basic principle components of fermentation technology. Types of microbial

culture and its growth kinetics– Batch, Fedbatch and Continuous culture

UNIT II (Total Topics -12 and Hrs-10)

Design of bioprocess vessels- Significance of Impeller, Baffles, Sparger; Types of

culture/production vessels- Airlift; Cyclone Column; Packed Tower and their application in

production processes. Principles of upstream processing – Media preparation, Inocula

development and sterilization

UNIT- III (Total Topics -5 and Hrs-10)

Introduction to oxygen requirement in bioprocess; mass transfer coefficient; factors affecting

KLa. Bioprocess measurement and control system with special reference to computer aided

process control.

UNIT-IV (Total Topics -8 and Hrs-10)

Introduction to downstream processing, product recovery and purification. Effluent treatment-

primary and secondary. Microbial production of ethanol, amylase, lactic acid and Single Cell

Proteins.

Course Outcomes (COs)

TBBT-501 CO1. Inculcate technical skills related to designing of bioreactors/fermentors, media

preparation and related downstream techniques.

TBBT-501 CO2. Understand the design of bioprocess vessels and needs of various parts of

fermenter along with their operation in laboratory as well as industrial level.

TBBT-501 CO3. Designing and development medium for microbial cell cultivation for

conducting various applications of fermentation in industry and research.

TBBT-501 CO4. Development of computational skills to control bioprocess parameters and

inculcate knowledge related to fermented product recovery and purification in industrial scale. References:

Books

1. Casida, L.E., Industrial Microbiology,Wiley Eastern Limited,1991, 1st edition.

2. Crueger, W. and Crueger, A., Biotechnology: A textbook of Industrial Microbiology, Panima

Publishing Co. New Delhi, 2000, 2nd edition.

Page 114: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

3. Patel A.H. ,Industrial Microbiology.,Macmillan India Limited, 19961st edition

Mode of Evaluation

Internal (MM-40) External (MM-60)

Component Sessional -1 Sessional -2 Assignments End Semester Examination

Weightage 10 10 20 60

CO-PO Matrix Bio-process TechnologyTBBT-501

Course Outcome PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PSO

1

PSO

2

PSO

3

PSO

4

TBBT-501CO 1. 3 1 2 1 1 - - 3 3 2 1

TBBT-501CO 2. 3 2 2 - 1 - - 3 3 - 1

TBBT-501CO 3. 3 - 1 - 1 - - 2 2 2 -

TBBT-501CO 4. 3 2 1 1 2 - - 3 2 2 1

Average CO(TBBT-

501) 3 1.6 1.5 1 1.2 - - 2.75 2.5 2 1

Page 115: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc. (H) Biotechnology Programme Code 05

Course Code PBBT-501 Credit 2

Year/Sem 3/5 L-T-P 0-0-4

Course Name Bio-process Technology Lab

Objectives of the Course:

1. To impart basic knowledge of experimental techniques associated with aseptic process,

mediapreparation and related upstream and downstream process.

2. To provide the knowledge related to needs of various parts of fermenter and their operation

inlaboratory as well as industrial level.

3. To understand conditions that influence growth and fermentation in industry and research.

4. To gain the knowledge of scale-up in fermentation process from laboratory scale to industrial

scale.

PRACTICALS

1. Plotting of bacterial growth curve.

2. Calculation thermal death point (TDP) of a microbial sample.

3. Production and analysis of ethanol.

4. Production and analysis of amylase.

5. Production and analysis of lactic acid.

6. Isolation of industrially important microorganism from natural resource.

Course Outcomes (COs)

PBBT-501 CO1. Impart Knowledge regarding of microbial growth kinetics and fermentation

reaction in laboratory for production of value added products.

PBBT-501 CO2. Acquire operating skills for fermenter and the needs of various parts of

fermenter in laboratory as well as industrial level.

PBBT-501 CO3. Acquire knowledge regarding preparation of fermentative media for microbial

cell cultivation for conducting various applications of fermentation in industry and research.

PBBT-501 CO4. Development of knowledge regarding isolation of industrially important

microorganism from natural resource for fermentation process in laboratory scale. References:

Books

1. Casida, L.E., Industrial Microbiology,Wiley Eastern Limited,1991, 1st edition.

2. Crueger, W. and Crueger, A., Biotechnology: A textbook of Industrial Microbiology, Panima

Publishing Co. New Delhi, 2000, 2nd edition.

3. Patel A.H. Industrial Microbiology.,Macmillan India Limited, 1996, 1st edition

Mode of Evaluation

Internal (MM-25) External (MM-25)

Component Internal End Semester Examination

Weightage 25 25

CO-PO Matrix Bio-process TechnologyLab PBBT-501

Page 116: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Course Outcome PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PSO

1

PSO

2

PSO

3

PSO

4

PBBT-501CO 1. 2 1 3 1 2 - - 2 1 2 1

PBBT-501CO 2. 2 - 3 - 1 - - 2 3 - 1

PBBT-501CO 3. 2 - 3 - 1 - - 2 2 2

PBBT-501CO 4. 3 2 3 1 1 - - 3 2 2 1

Average CO(PBBT-

501) 2.2 1.5 3 1 1.2 - - 2.2 2 2 1

Page 117: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc. (H) Biotechnology Programme Code 05

Course Code TBBT-502 Credit 4

Year/Sem 3/5 L-T-P 4-0-0

Course Name Recombinant DNA Technology

Objectives of the Course:

1. This course offer students to learn the gene basis molecular tools and techniques used in

genetic engineering to improvement the future.

2. To make students learn the application of recombinant DNA technology in the field of

modern biomedical science, agriculture and environment.

3. To familiarize the students with all practical biotechniques required for creating a

recombinant DNA molecule and transforming the appropriate host cell to check the

expression of recombinant DNA.

4. To provides theoretical and practical bases to properties and applications of DNA cutting and

modifying enzymes, cloning vectors and strategies, host genotype specificities screening of

recombinant transformants.

UNIT I (Total Topics- 18 and Hrs- 10)

Molecular tools and applications -restriction enzymes, ligases, polymerases, alkaline

phosphatase, Episomes, Plasmids and other cloning vectors (Bacteriophage-derived vectors,

artificial chromosomes), Expression vectors, Gene Recombination and Gene transfer:

Transformation, Microinjection, Electroporation, Ultrasonication. Principle and applications of

Polymerase chain reaction (PCR), Primer designing.

UNIT II (Total Topics -11 and Hrs-10)

Restriction and modification system, restriction mapping. Southern and Northern hybridization.

Preparation and comparison of Genomic and cDNA library, screening of recombinants, reverse

transcription. Genome mapping.

UNIT- III (Total Topics -13 and Hrs-10)

Random and site-directed mutagenesis: Primer extension and PCR based methods of site directed

mutagenesis, Random mutagenesis, Gene shuffling, Chromosomal Walking, Chromosomal

Jumping. Applications of Genetic Engineering: Production and applications of transgenic mice,

role of ES cells in gene targeting in mice.

UNIT-IV (Total Topics -9 and Hrs-10)

Genetic engineering in plants: Use of Agrobacterium tumefaciens and Arhizogenes, Ti plasmids-

structural components, Strategies for gene transfer to plant cells, Direct DNA transfer to plants,

Gene targeting in plants, Use of plant viruses as episomal expression vectors.

Course Outcomes (COs)

TBBT-502 CO1. Develop good understanding of various modern tools, and techniques related

to recombinant DNA molecule technology applicable in scientific research advancement.

TBBT-502 CO2.Apply the basic and advanced recombinant DNA techniques such as gene

therapy which explore research in higher education and applicable in the modern medicinal

treatment system.

TBBT-502 CO3. Identify and analyze various tools and techniques utilized recombinant DNA

technology.

TBBT-502 CO4.Identify the ethical values related to transgenic and recombinant DNA

technology.

Page 118: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

References:

Books

1. Brown, T.A. , Gene Cloning and DNA Analysis, Blackwell Publishing, Oxford U.K.,2006 5th

edition.

2. Clark D.P. and Pazdernik, N.J., Biotechnology-Applying the Genetic Revolution, Elsevier

Academic Press, USA, 2009.

3. Glick B.R., and Pasternak, J.J., Molecular Biotechnology- Principles and Applications of

recombinant DNA, 2003 ASM Press, Washington.

4. Primrose S.B. and Twyman, R.M., Principles of Gene Manipulation and Genomics, Blackwell

Publishing, Oxford, U.K. 2006 7thedition.

5. Sambrook J., Fritsch, E. F. and Maniatis T., Molecular Cloning-A Laboratory Manual., Cold

Spring Harbor Laboratory Press. 2001 3rdedition.

Mode of Evaluation

Internal (MM-40) External (MM-60)

Component Sessional -1 Sessional -2 Assignments End Semester Examination

Weightage 10 10 20 60

CO-PO Matrix Recombinant DNA Technology TBBT-502

Course Outcome PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PSO

1

PSO

2

PSO

3

PSO

4

TBBT-502CO 1. 3 2 1 - - - 2 2 1 2 2

TBBT-502 CO 2. 2 - 1 - - - - 1 2 - -

TBBT-502 CO 3. 2 - - - 2 1 - 2 2 1 2

TBBT-502 CO 4. - - 1 3 - - - - - - -

Average CO(TBBT-

502) 2.3 2.0 1.0 3.0 2.0 1.0 2.0 1.6 1.6 1.5 2.0

Page 119: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc. (H) Biotechnology Programme Code 05

Course Code PBBT-502 Credit 2

Year/Sem 3/5 L-T-P 0-0-4

Course Name Recombinant DNA Technology Lab

Objectives of the Course:

1. This course offer students to learn molecular basis tools used in genetic engineeringto

improve the objects.

2. To identify the ethical values related to transgenic and recombinant DNA technology.

3. To develop team effort practices within students with experiments of recombinant

4. DNA technology in the field of modern biomedical science, agriculture.

5. Demonstrate the experimental techniques related to recombinant DNA molecule

andexpression of recombinant DNA.

PRACTICALS

1. Molecular weight determination of isolated DNA samples.

2. Molecular weight determination of isolated protein samples.

3. Restriction digestion of DNA

4. To study about preparation of competent cells

5. Transformation of competent cells.

6. Demonstration of PCR.

7. Demonstration of mutagenesis.

Course Outcomes (COs)

PBBT-502 CO1. Identify research prospects of recombinant DNA technology..

PBBT-502CO2. Interpret the test hypotheses, analyze the data of RDT by using modern

methods.

PBBT-502 CO3. Develop laboratory skills for academic and professional enhancement

PBBT-502 CO4. Apply the basic and advanced recombinant DNA techniques experiments

applicable in scientific research & different industries. References:

Books

1. T. A. Brown, Gene Cloning and DNA Analysis: An Introduction, Wiley; 6th edition

2. Sandy B. Primrose, Principles of Gene Manipulation Paperback, Wiley-Blackwell; 6th

Edition

Mode of Evaluation

Internal (MM-25) External (MM-25)

Component Internal End Semester Examination

Weightage 25 25

Page 120: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

CO-PO Matrix Recombinant DNA Technology Lab PBBT-502

Course Outcome PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PSO

1

PSO

2

PSO

3

PSO

4

PBBT-502CO 1. 3 1 - 2 1 2 1 2 3 2 2

PBBT-502 CO 2. 2 1 2 - 2 2 - - 2 1 -

PBBT-502 CO 3. 2 1 - - 2 - - 2 3 1 1

PBBT-502 CO 4. 2 1 1 1 - - 2 - 2 2 3

Average CO(PBBT-

502) 2.2 1.0 1.5 1.5 1.6 2.0 1.5 2.0 2.5 1.5 2.0

Page 121: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc. (H) Biotechnology Programme Code 05

Course Code TBBT-503 (I) Credit 4

Year/Sem 3/5 L-T-P 4-0-0

Course Name Bioinformatics

Objectives of the Course:

1. To identity and understand concept of bioinformatics, introduction to history of

bioinformatics along with access to widespread bioinformatics resources on web.

2. To classify and compare different biological databases along with utilization of

bioinformatics tools in bioanalytical techniques including chromatography, mass

spectrometry, Microarray, etc.

3. To comprehend designing and working of bioinformatics softwares and programmes such as

CLUSTAL W, mutation and substitution matrices utilized in sequence alignment,

phylogenetic analysis.

4. To understand how to search databases, homology modelling, gene annotation, utilization of

BLAST and FASTA and application of various gene identification tools.

UNIT I (Total Topics- 10 and Hrs- 10)

History of Bioinformatics. The notion of Homology. Primary and Secondary Databases,

Composite database, Sequence Information Sources, NCBI, EMBL, GENBANK, Understanding

the structure of each source and using it on the web.

UNIT II (Total Topics -12 and Hrs-10)

Protein Information Sources, PDB, SWISSPROT, TREMBL. Introduction of Data Generating

Techniques and Bioinformatics problem posed by them-Restriction Digestion, Chromatograms,

Blots, PCR, Microarrays, Mass Spectrometry.

UNIT- III (Total Topics -10 and Hrs-10)

Sequence and Phylogeny analysis, Detecting Open Reading Frames, Outline of sequence

Assembly, Mutation/Substitution Matrices, Local and Pairwise sequence Alignments, Multiple

Sequence Alignment- CLUSTALW, Phylogenetic Analysis.

UNIT-IV (Total Topics -10 and Hrs-10)

Searching Databases: SRS, Entrez, Unigene, Sequence Similarity Searches-BLAST, FASTA,

Homology Modeling, Data Submission. Genome Annotation: Pattern and repeat finding, Gene

identification tools.

Course Outcomes (COs)

TBBT-503 (I) CO1. Exhibit sound knowledge pertaining to concept and notion of

bioinformatics alongwith comprehending web based utilization of bioinformatics resources

TBBT-503 (I) CO2. Conceptual comprehension and technical application of various

bioinformatics based tools to assess their utilization in bioanalytical techniques including

chromatography, mass spectrometry, microarray, restriction digestion, PCR.

TBBT-503 (I) CO3. Identify and apply softwares utilized in bioinformatics for sequence

alignment phylogenetic analysis and assess applicability of same in biological research.

TBBT-503 (I) CO4. Access applicability of widespread bioinformatics techniques such as

homology modeling, gene identification tool, searching databases, genome annotation and

simultaneously identify research oriented potential of bioinformatics.

References:

Page 122: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Books

1. Ghosh, Z. and Bibekanand, M., Bioinformatics: Principles and Application, Oxford

University Press., 2008.

2. Pevsner, J., Bioinformatics and Functional Genomics.,Wiley-Blackwell,2009, II Edition.

3. Campbell ,A. M., and Heyer, L. J., Discovering Genomics, Proteomics and Bioinformatics,

Benjamin Cummings, 2006,IInd Edition.

Mode of Evaluation

Internal (MM-40) External (MM-60)

Component Sessional -1 Sessional -2 Assignments End Semester Examination

Weightage 10 10 20 60

CO-PO Matrix Bioinformatics TBBT-503 (I)

Course Outcome PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PSO

1

PSO

2

PSO

3

PSO

4

TBBT-503 (I) CO 1. 3 2 2 - 3 - - 3 - - -

TBBT-503 (I) CO 2. 3 1 3 - 3 - 2 3 2 - 2

TBBT-503 (I) CO 3. 3 1 2 - 3 - 2 3 2 - 1

TBBT-503 (I) CO 4. 3 1 1 - 3 - - 3 - - -

Average CO(TBBT-503

(I)) 3 1.2 2

- 3

- 2 3 2 - 1.5

Page 123: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc. (H) Biotechnology Programme Code 05

Course Code PBBT-503 (I) Credit 1

Year/Sem 3/5 L-T-P 0-0-2

Course Name Bioinformatics Lab

Objectives of the Course:

1. To understand utilization of various web resources and their significance and application.

2. To comprehend organization of biological databases, format of data stored and process of

data retrieval.

3. To develop technical skill for implementation of various softwares and tools for sequence

alignment.

4. To learn the technique / process for studying, determining 3D structure of proteins. PRACTICALS

1. Understanding and use of various web resources: EMBL, Genbank, Entrez, Unigene, PIR

2. Retrieval of information from nucleotide databases, structural databases.

3. Understanding and using: PDB, Swissprot, TREMBL

4. Using various BLAST and interpretation of results..

5. Multiple sequence alignment using CLUSTAL W.

6. Protein 3D structure determination and Visualization.

7. Identify the concept of scoring matrix.

Course Outcomes (COs)

PBBT-503 (I) CO 1.Acquire knowledge and depict skill to explore widespread resources

accessible through internet and utilize same for data retrieval and interpretation.

PBBT-503 (I) CO 2. Demonstrate an understanding of organization and utilization of biological

databases, search engines and identify their applicability in biological research.

PBBT-503 (I) CO 3.Exhibit technical skill to utilize various IT derived programmes, softwares

utilized in biological studies alongwith interpretation of data.

PBBT-503 (I) CO 4. Depict ability to study, analyze and determine 3D structure of proteins

(biomolecules) and the significance of same. References:

Books

1. Ghosh, Z. and Bibekanand, M., Bioinformatics: Principles and Application, Oxford University

Press., 2008.

2. Pevsner, J., Bioinformatics and Functional Genomics.,Wiley-Blackwell,2009, 2ndEdition.

3. Campbell A. M., and Heyer, L. J., Discovering Genomics, Proteomics and Bioinformatics,

Benjamin Cummings, 2006,2ndEdition.

Mode of Evaluation

Internal (MM-25) External (MM-25)

Component Internal End Semester Examination

Weightage 25 25

Page 124: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

CO-PO Matrix Bioinformatics Lab PBBT-503 (I)

Course Outcome PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PSO

1

PSO

2

PSO

3

PSO

4

PBBT-503 (I) CO 1. 2 - 2 - 2 - 1 2 3 - -

PBBT-503 (I) CO 2. 2 - 3 - 3 - 2 2 3 - 2

PBBT-503 (I) CO 3. 3 - 3 - 3 - 1 3 3 - 1

PBBT-503 (I) CO 4. 1 - 2 - 2 - - 1 2 - -

Average CO(PBBT-503

(I)) 2 - 2.5

- 2.5

- 1.3 2 2.75 - 1.5

Page 125: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc (H) Biotechnology Programme Code 05

Course Code TBBT-503 (II) Credit 4

Year/Sem 3/5 L-T-P 4-0-0

Course Name IPR, Entrepreneurship, Bioethics & Biosafety

Objectives of the Course:

1. To provide basic scientific knowledge pertaining to safety principles of laboratory practices

in which potentially hazardous materials are manipulated or handled.

2. To develop ethical skills through world-wide case studies in healthcare and environment.

3. To provide basic knowledge for development of moral and professional ethics in the field of

healthcare.

4. To give scientific knowledge regarding protection of inventions and technologies with

context of entrepreneurship.

UNIT I (Total Topics-10 and Hrs-10)

Introduction to Indian Patent Law. Intellectual property rights. Patents, Copyrights, Trademarks,

Trade secrets, Patenting of GMOs. Process of filing a patent: Process and product patent, patent

application and guidelines, Prerequisites of filing a patent, Novelty, Utility.

UNIT II (Total Topics-10 and Hrs-10)

Entrepreneurship: Selection of a product, line, design and development processes, economics on

material and energy requirement, stock the product and release the same for making etc. The

basic regulations of excise: Demand for a given product, feasibility of its production under given

constraints of raw material, energy input, financial situations export potential etc.

UNIT- III (Total Topics-5 and Hrs-7)

Bioethics – Necessity of Bioethics, different paradigms of Bioethics – National & International.

Ethical issues against the molecular technologies.

UNIT-IV (Total Topics-5 and Hrs-7)

Biosafety– Introduction to biosafety and health hazards concerning biotechnology. Introduction

to the concept of containment level and Good Laboratory Practices (GLP) and Good

Manufacturing Practices (GMP).

Course Outcomes (COs)

TBBT-503 (II) CO1. Development of skills about broader global ethical issues through case

studies in healthcare.

TBBT-503 (II) CO2. Impart knowledge and skills to develop moral and professional ethics

related to healthcare as well as to become entrepreneurs.

TBBT-503 (II) CO3. Development of knowledge regarding protection of their inventions and

technologies

TBBT-503 (II) CO4. Development of scientific knowledge regarding laboratory and

manufacturing practices for safety regulation in disposal of hazardous chemicals in the

environment.

References:

Books

1. Entrepreneurship: New Venture Creation : David H. Holt

2. Patterns of Entrepreneurship : Jack M. Kaplan

Page 126: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

3. Entrepreneurship and Small Business Management: C.B. Gupta, S.S. Khanka, Sultan Chand

Sons.

4. Sateesh M.KBioethics and Biosafety, I. K. International Pvt Ltd. 2010

5. Sree Krishna V Bioethics and Biosafety in Biotechnology, New age international Publisher

Mode of Evaluation

Internal (MM-40) External (MM-60)

Component Sessional -1 Sessional -2 Assignments End Semester Examination

Weightage 10 10 20 60

CO-PO Matrix IPR, Entrepreneurship, Bioethics & Biosafety TBBT-503 (II)

Course Outcome PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PSO

1

PSO

2

PSO

3

PSO

4

TBBT-503 (II) CO 1. 2 - - 2 - - 1 2 - 1 -

TBBT-503 (II) CO 2. 3 - - 2 - - - 1 2 2 1

TBBT-503 (II) CO 3. 3 2 1 - - - - 2 - 1 -

TBBT-503 (II) CO 4. 3 - - 1 - - - 2 1 1 1

Average CO (TBBT-503

(II)) 2.8 2 1 - - - 1 1.8 1.5 1.3 1

Page 127: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc (H) Biotechnology Programme Code 05

Course Code PBBT-503 (II) Credit 1

Year/Sem 3/5 L-T-P 0-0-2

Course Name IPR, Entrepreneurship, Bioethics & Biosafety Lab

Objectives of the Course:

1. Development of scientific knowledge regarding laboratory and manufacturing practices for

entrepreneurship improvement.

2. To develop ethical skills and laboratory skills for good laboratory practices.

3. To provide scientific knowledge regarding safety principles to laboratory practices in which

potentially hazardous materials are manipulated or handled through case study.

4. To provide basic scientific knowledge for filing patent in India.

PRACTICALS

1. Proxy filing of Indian Product patent

2.Proxy filing of Indian Process patent

3.Planning of establishing a hypothetical biotechnology industry in India

4.A case study on clinical trials of drugs in India with emphasis on ethical issues.

5.Case study on women health ethics.

6.Case study on medical errors and negligence.

7.Case study on handling and disposal of radioactive waste

Course Outcomes (COs)

PBBT-503 (II) CO 1.Impart knowledge and skills to develop moral and professional ethics

related to healthcare through various case studies.

PBBT-503 (II) CO 2. Development of scientific knowledge regarding laboratory and

manufacturing practices for safety regulation in disposal of hazardous chemicals in the

environment through case Studies.

PBBT-503 (II) CO 3. Demonstrate the process under IP India for filing of process and product

patent for the protection of their inventions and technologies.

PBBT-503 (II) CO 4. Acquire knowledge regarding protection of inventions, publications and

technologies through case studies.

References:

Books

1. Sateesh, M.K., Bioethics and Biosafety, I. K. International Pvt Ltd, 2010.

2. Krishna, V .S., Bioethics and Biosafety in Biotechnology, New age international publisher,

2010.

3. David H. Holt: Entrepreneurship: New Venture Creation

Page 128: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Mode of Evaluation

Internal (MM-25) External (MM-25)

Component Internal End Semester Examination

Weightage 25 25

CO-PO Matrix IPR, Entrepreneurship, Bioethics & Biosafety Lab PBBT 503 (II)

Course Outcome PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PSO

1

PSO

2

PSO

3

PSO

4

PBBT-503 (II) CO 1. 2 - - 1 - - 1 1 - 1 -

PBBT-503 (II) CO 2. 2 - - 1 - - - 1 2 2 1

PBBT-503 (II) CO 3. 2 1 1 - - - - 1 - 1 -

PBBT-503 (II) CO 4. 2 - - 1 - - - 1 1 1 1

Average CO (PBBT-

503 (II)) 2 1 1 - - - 1 1 1.5 1.2 1

Page 129: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name

B.Sc. (H) Biotechnology Programme Code 05

Course Code TBBT-503 (III) Credit 4

Year/Sem 3/5 L-T-P 4-0-0

Course Name Microbial Physiology

Objectives of the Course:

1. To understand the basic microbial structure, function and to study the microbial growth

response to variable factor like temperature, heat, pH and oxygen etc.

2. To understand the nutrient assimilation and transport in bacteria.

3. To learn basic knowledge of aerobic and anaerobic respiration

4. To prepare students to learn about different aspects of photosynthesis and carbon dioxide

fixation.

UNIT I (Total Topics- 10 and Hrs- 10)

Nutritional classification of microorganisms based on carbon, energy and electron sources,

Metabolite Transport, Diffusion: Passive and facilitated, Primary active and secondary active

transport, Group translocation (phosphotransferase system), symport, antiport and uniport,

electrogenic and electro neutral transport, transport of Iron.

UNIT II (Total Topics -12 and Hrs-10)

Microbial Growth. Definition of growth, balanced and unbalanced growth, growth curve, the

mathematics of growth-generation time, specific growth rate, batch and continuous culture,

synchronous growth, diauxie growth curve. Measurement of microbial growth. Measurement of

cell numbers, cell mass and metabolic activity.

UNIT- III (Total Topics -10 and Hrs-10)

Effect of the environment on microbial growth

Temperature- temperature ranges for microbial growth, classification based on temperature

ranges and adaptations, pH-classification based on pH ranges and adaptations, solutes and water

activity, oxygen concentration, radiation and pressure. Chemolithotrophic metabolism,

Physiological groups of aerobic and anaerobic chemolithotrophs. Hydrogenoxidizing bacteria

and methanogens.

UNIT-IV (Total Topics -10 and Hrs-10)

Phototrophic metabolism. Historical account of photosynthesis, diversity of phototrophic

bacteria, anoxygenic and oxygenic photosynthesis, photosynthetic pigments: action and

absorption spectrum, type, structure and location, physiology of bacterial photosynthesis: light

reactions, cyclic and non-cyclic photophosphorylation. Carbon dioxide fixation, Calvin cycle and

reductive TCA cycle.

Course Outcomes (COs)

TBBT-503 (III) CO1Understand the growth characteristics of the microorganisms capable of

growing under different environmental condition of temperature, pH, oxygen, solute and water.

TBBT-503 (III) CO2.Acquire the knowledge of the growth characteristics of the

microorganisms which require different nutrient for growth and transport.

TBBT-503 (III)CO3.Learn the concepts of photosynthesis, aerobic and anaerobic respiration

and how these are manifested in the form of different metabolic pathways in microorganisms.

Page 130: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

TBBT-503 (III) CO4. Explore about basics of chemolithotrophic, phototrophic microbes and

their metabolism.

References:

Books

1. Gottschalk G. Bacterial Metabolism. Springer Verlag 1986 2nd Edition

2. Madigan M.., Martinko JM and Parker J. Brock Biology of Microorganisms.Pearson/

Benjamin Cummings.200310th Edition

3. Moat A.G. and Foster J.W. Microbial Physiology. John Wiley & Sons. 2002 4TH Edition.

4. Reddy S.R. and Reddy S.M. Microbial Physiology. Scientific Publishers India 2005

Mode of Evaluation

Internal (MM-40) External (MM-60)

Component Sessional -1 Sessional -2 Assignments End Semester Examination

Weightage 10 10 20 60

CO-PO Matrix Microbial Physiology TBBT-503 (III)

Course Outcome PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7 PSO1 PSO2 PSO3 PSO4

TBBT-503 (III) CO 1. 3 - - - - - 2 2 - 1 -

TBBT-503 (III) CO 2. - 1 - - - - - - 1 - -

TBBT-503 (III) CO 3. - - 1 - - 1 - - - 1 -

TBBT-503 (III) CO 4. - - - - - 1 - - - - 1

Average CO (TBBT-

503 (III)) 3 1 1 - - 1 2 2 1 1 1

Page 131: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc (H) Biotechnology Programme Code 05

Course Code PBBT-503 (III) Credit 1

Year/Sem 3/5 L-T-P 0-0-2

Course Name Microbial Physiology Lab

Objectives of the Course:

1.To learn the growth curve of E coli, and calculation of generation time and specific growth

rate.

2.To understand the effect of temperature, pH, salt and carbon and nitrogen sources on growth

of E.coli.

3.To learn the basic technique of alcohol fermentation and its application.

4.To have basic understanding of the thermal death time and decimal reduction time of E. Coli

PRACTICALS

1.To study and plot the growth curve of a bacteria using turbidometric method and to calculate

specific growth rate and generation time.

2.To study and plot the growth curve of a bacteria by radial growth measurements.

3.To study the effect of pH on the growth of a bacteria

4.To study the effect of temperature of a bacteria by dry weight method.

5.Demonstration of the thermal death time and decimal reduction time of a bacteria.

Course Outcomes (COs)

PBBT-503 (III) CO1. Avail scientific knowledge pertaining to growth and growth rate in

bacteria

PBBT-503 (III) CO2. Impart techniques which will help students to learn the effect of variable

external factors in its growth and development.

PBBT-503 (III) CO3.Learn and developed the techniques of alcohol fermentation.

PBBT-503 (III) CO4. Inculcate the scientific knowledge for determination of thermal death

time and decimal reduction time in E. Coli.

References:

Books

1.Holt D.H., Entrepreneurship: New Venture Creation, PHI, 1998.

2.Kaplan, J.M., Patterns of Entrepreneurship, Wiley, 2016.

3.Gupta, C.B. and Khanka, S.S., Entrepreneurship and Small Business Management, Sultan

Chand & Sons.

Mode of Evaluation

Internal (MM-25) External (MM-25)

Component Internal End Semester Examination

Weightage 25 25

Page 132: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

CO-PO Matrix Microbial physiology PBBT-503 (III)

Course Outcome PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PSO

1

PSO

2

PSO

3

PSO

4

PBBT-503(III) CO 1. 2 - - - - - 2 2 - - -

PBMC-503(III) CO 2. - 1 - - - - - - 2 - -

PBMC-503(III) CO 3. - 2 - 2 - - - - - 1 -

PBMC-503(III) CO 4. - - - 1 - 2 - - - - 1

Average CO (PBBT-

503 (III)) 2 1.5 - 1.5 - 2 2 2 2 1 1

Page 133: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc (H) Biotechnology Programme Code 05

Course Code TBBT-503 (IV) Credit 4

Year/Sem 3/5 L-T-P 4-0-0

Course Name Biostatistics

Objectives of the Course:

1. To expose the student to fundamental properties and concepts of modern statistical theory.

2. To understand the statistical techniques of data collection, analysis and interpretation.

3. To have basic understanding of correlation and regression.

4. To develop analytic thinking to solve biological problems through bio-statistical techniques.

UNIT I (Total Topics-10 and Hrs-10)

Types of Data, Collection of data; Primary & Secondary data, Classification and Graphical

representation of Statistical data. Measures of central tendency. Measures of Skewness and

Kurtosis.

UNIT II (Total Topics-10 and Hrs-10)

Probability classical & axiomatic definition of probability, Theorems on total and compound

probability), Elementary ideas of Binomial, Poisson and Normal distributions.

UNIT- III (Total Topics-5 and Hrs-7)

Methods of sampling, confidence level, critical region, testing of hypothesis and standard error,

large sample test and small sample test. Problems on test of significance, t-test, chi-square test

for goodness of fit and analysis of variance (ANOVA)

UNIT-IV (Total Topics-5 and Hrs-7)

Correlation and Regression. Emphasis on examples from Biological Sciences.

Course Outcomes (COs)

TBBT-503 (IV) CO1. Demonstrate an understanding of the central concepts of modern

statistical theory and their probabilistic foundation.

TBBT-503 (IV) CO2. Collect data relating to variable/variables which will be examined and

calculate descriptive statistics from these data. Further identify convenient sample by using

sampling theory.

TBBT-503 (IV) CO3. Perform statically analyses of biological datasets and relate the results to

core principles in biology.

TBBT-503 (IV) CO4. Demonstrate familiarity with intermediate statistical theory and methods

of Correlation and Linear Regression.

References:

Books

1. Le, C.T. Introductory biostatistics. 1st edition, John Wiley, USA 2003

2. Glaser, A.N High Yield T.M. Biostatistics. Lippincott Williams and Wilkins, USA 2001

3. Edmondson, A. and Druce, D. Advanced Biology Statistics, Oxford University Press 1996

4. Danial, W. Biostatistics: A foundation for Analysis in Health Sciences, John Wiley and Sons

Inc. 2004

Page 134: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Mode of Evaluation

Internal (MM-40) External (MM-60)

Component Sessional -1 Sessional -2 Assignments End Semester Examination

Weightage 10 10 20 60

CO-PO Matrix Biostatistics TBBT-503 (IV)

Course Outcome PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PSO

1

PSO

2

PSO

3

PSO

4

TBBT-503 (IV) CO 1. 3 3 1 - - - 2 3 1 - 1

TBBT-503 (IV) CO 2. 3 3 3 - 2 - 2 2 3 2 2

TBBT-503 (IV) CO 3. 3 3 2 - 1 - 1 2 3 1 2

TBBT-503 (IV) CO 4. - 2 - - - - - 2 2 1 -

Average CO (TBBT-503

(IV)) 3 2.7 1.5

1.5

1.6 1.7 2.2 1.3 1.6

Page 135: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc (H) Biotechnology Programme Code 05

Course Code PBBT-503 (IV) Credit 1

Year/Sem 3/5 L-T-P 0-0-2

Course Name Biostatistics Lab

Objectives of the Course:

1. To demonstrate deep knowledge in graphical representation.

2. To acquire basic knowledge on distribution binomial Poisson normal

3. To inculcate students knowledge of different chi square test

PRACTICALS

1. Based on graphical Representation

2. Based on measures of Central Tendency & Dispersion

3. Based on Distributions Binomial Poisson Normal

4. Based on t, f, z and Chi-square

Course Outcomes (COs)

PBBT-503 (IV) CO 1. Demonstration of measure of central tendency & dispersion

PBBT-503 (IV) CO 2. Acquire knowledge related to solving biological data through graphical

representation

PBBT-503 (IV) CO 3. Acquire basic knowledge on distribution binomial Poisson normal

References:

Books

1. Glaser, A.N High Yield T.M. Biostatistics. Lippincott Williams and Wilkins, USA 2001

Mode of Evaluation

Internal (MM-25) External (MM-25)

Component Internal End Semester Examination

Weightage 25 25

CO-PO Matrix Biostatistics Lab PBBT 503 (IV)

Course Outcome PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PSO

1

PSO

2

PSO

3

PSO

4

PBBT-503 (IV) CO 1. 1 3 2 - - - 2 - 3 1 -

PBBT-503 (IV) CO 2. 1 3 1 - - - 1 - 3 2 -

PBBT-503 (IV) CO 3. 1 2 1 - - - 1 - 1 1 -

Average CO (PBBT-503

(IV)) 1 2.6 1.3 - - - 1.3 - 2.3 1.3 -

Page 136: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc (H) Biotechnology Programme Code 05

Course Code TBBT-504 (I) Credit 4

Year/Sem 3/5 L-T-P 4-0-0

Course Name Ecology and Environment Management

Objectives of the Course:

1. To acquire master foundational knowledge enabling them to have life–long learning related

to one’s surroundings.

2. To develop critical thinking skills in relation to environmental affairs and articulate

multidisciplinary context of the subject.

3. To acquire knowledge about the role of Biotechnology in protection and preservation of

environment

4. To interpret and propose solutions for effective management of different types of

environmental pollution

UNIT I (Total Topics-10 and Hrs-10)

Our Environment: Geological consideration of Atmosphere, Hydrosphere, Lithosphere Scope of

Ecology. Development & Evolution of Ecosystem. Principles & Concepts of Ecosystem.

Structure of ecosystem. Strata of an ecosystem. Types of ecosystem including habitats.

Cybernetics & Homeostasis. Biological control of chemical environment. UNIT II (Total Topics-15 and Hrs-10)

Energy transfer in an Ecosystem. Food chain, food web, Energy budget, Production &

decomposition in a system. Ecological efficiencies, Trophic structure & energy pyramids,

Ecological energetic, principles pertaining to limiting factors, Bio-geochemical cycles (N,C,P

cycles).

UNIT- III (Total Topics-14 and Hrs-7)

Pollution & environmental Health related to Soil, Water, Air, Food, Pesticides, Metals, Solvents,

Radiations, Carcinogen, Poisons. Detection of Environmental pollutant. Indicators & detection

systems. Bio-transformation, Plastic, Aromatics, Hazardous wastes Environmental cleanup :

Case studies

UNIT-IV (Total Topics-6 and Hrs-7)

Environmental biotechnologies, Biotechnologies in protection and preservation of environment.

Bioremediation, Waste disposal.

Course Outcomes (COs)

TBBT-504 (I) CO1. Master foundational knowledge enabling them to have life–long learning

related to one’s surroundings.

TBBT-504 (I) CO2. Develop critical thinking skills in relation to environmental affairs and

articulate multidisciplinary context of the subject.

TBBT-504 (I) CO3. Acquire knowledge about role of biotechnology in protecting the

environment

TBBT-504 (I) CO4. Interpret and propose solutions for effective management of different types

of environmental pollution.

References:

Books

1.Chapman, J.L., Reiss, M.J. Ecology: Principles and applications Cambridge University Press

Page 137: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

2nd Edition 1999

2.Joseph, B., Environmental studies, Tata Mc Graw Hill.

3.Michael A. Basics of environmental science, Routledge Press.

4. Mohapatra. Textbook of environmental biotechnology IK publication.

5. Thakur, I. S. Environmental Biotechnology, I K Publication.

Mode of Evaluation

Internal (MM-40) External (MM-60)

Component Sessional -1 Sessional -2 Assignments End Semester Examination

Weightage 10 10 20 60

CO-PO Matrix Ecology and Environment Management TBBT-504 (I)

Course Outcome PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PSO

1

PSO

2

PSO

3

PSO

4

TBBT-504 (I) CO 1. 1 - - 2 - 1 2 1 - 3 1

TBBT-504 (I) CO 2. 1 - - 2 - 1 2 1 - 3 1

TBBT-504 (I) CO 3. 1 - - 2 - 1 2 1 - 3 1

TBBT-504 (I) CO 4. 1 - - 2 - 1 2 1 - 3 1

Average CO (TBBT-

504 (I)) 1 - - 2 - 1 2 1 - 3 1

Page 138: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc (H) Biotechnology Programme Code 05

Course Code PBBT-504 (I) Credit 1

Year/Sem 3/5 L-T-P 0-0-2

Course Name Ecology and Environment ManagementLab

Objectives of the Course:

1. To study about biotic and abiotic component of natural environment.

2. To understand the basic principle of Global positioning system.

3. To learn about endangered and threatened species present in wild.

4. To have basic knowledge of soil and its composition.

PRACTICALS

1. Study of all the biotic and abiotic components of any simple ecosystem- natural pond or

terrestrial ecosystem or human modified ecosystem.

2. Principle of GPS (Global Positioning System).

3. Study of the life table and fecundity table, plotting of the three types of survivorship curves

from the hypothetical data.

4. Study of the types of soil, their texture by sieve method and rapid tests for –pH, chlorides,

nitrates, carbonates and organic carbon

5. Study any five endangered/ threatened species- one from each class

Course Outcomes (COs)

PBBT-504 (I) CO 1. Develop a deep understanding of biotic and abiotic component of

ecosystem.

PBBT-504 (I) CO 2. Acquire knowledge of working and basic principle of Global positioning

system

PBBT-504 (I) CO 3. Develop laboratory skills for soil texture estimation by different

biochemical tests.

PBBT-504 (I) CO 4To inculcate knowledge about endangered and threatened species

References:

Books

1. Michael A. Basics of environmental science, Routledge Press.

Mode of Evaluation

Internal (MM-25) External (MM-25)

Component Internal End Semester Examination

Weightage 25 25

Page 139: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

CO-PO Matrix Ecology and Environment Management PBBT 504 (I)

Course Outcome PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PSO

1

PSO

2

PSO

3

PSO

4

PBBT-504 (I) CO 1. 2 - 2 1 2 - - 2 2 3 -

PBBT-504 (I) CO 2. 1 - 3 2 2 - - 2 2 2 -

PBBT-504 (I) CO 3. 1 - 2 1 2 - - 2 3 3 -

PBBT-504 (I) CO 4. - - 2 2 2 - - 2 2 3 -

Average CO (PBBT-

504 (I)) 1.3 - 2.2 1.5 2 - - 2 2.2 2.7 -

Page 140: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc. (H) Biotechnology Programme Code 05

Course Code TBBT-504 (II) Credit 4

Year/Sem 3/5 L-T-P 4-0-0

Course Name Environmental Biotechnology

Objectives of the Course:

1. To study current applications of biotechnology to environmental quality evaluation,

monitoring, and remediation of contaminated environments

2. To know the basic physiology of a microorganism and how their structure dictates their

function in the environment and understand the bases for microbial metabolism of

environmental contaminants

3. To know various techniques to modify and augment microorganisms in the laboratory and

environment

4. To understand the principles of bioremediation and phyto-remediation; basic design and

application of microbial fuel cells or new models to control pollution.

UNIT I (Total Topics- 15 and Hrs- 10)

Conventional fuels and their environmental impact – Firewood, Plant, Animal, Water, Coal and

Gas. Modern fuels and their environmental impact – Methanogenic bacteria, Biogas, Microbial

hydrogen Production, Conversion of sugar to alcohol Gasohol.

UNIT II (Total Topics -12 and Hrs-10)

Bioremediation of soil & water contaminated with oil spills, heavy metals and detergents.

Degradation of lignin and cellulose using microbes. Phyto-remediation. Degradation of

pesticides and other toxic chemicals by micro-organisms- degradation aromatic and chlorinates

hydrocarbons and petroleum products.

UNIT- III (Total Topics -8 and Hrs-10)

Treatment of municipal waste and Industrial effluents. Bio-fertilizers, Role of symbiotic and

asymbiotic nitrogen fixing bacteria in the enrichment of soil. Algal and fungal biofertilizers

(VAM).

UNIT-IV (Total Topics -8 and Hrs-10)

Bioleaching, Enrichment of ores by microorganisms (Gold, Copper and Uranium).

Environmental significance of genetically modified microbes, plants and animals.

Course Outcomes (COs)

TBBT-504 (II) CO 1.Prior acquaintance and introduction of environmental science and apply

these concepts within a coherent engineering-based framework to the broad areas of

environmental biotechnology.

TBBT-504 (II) CO 2.Edification of the environmental guidelines, agencies and acts, and

awareness about the environmental impact assessment by natural causes, GMO’s, man-made

activities.

TBBT-504 (II) CO 3. Proficiency to design, implement and analyze data and the technology to

overcome them- energy production and reduction of pollution.

TBBT-504 (II) CO 4.The implication of scientific principles to design new models concerning

Page 141: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

modern biotechnology trends, benefit-cost analysis, better management of biotechnologies and

environmental control of the biotechnology industry.

References:

Books

1. Jordening, Hans-Joachim and Winter, Jesef , Environmental Biotechnology – Concepts and

Applications, Wiley-Blackwell, 2005.

2. Alicia, L. Spencer, R.D. and John, F.T., Spencer, Environmental Microbiology: Methods and

Protocols, Humana Press, 2004 4th Edition.

Mode of Evaluation

Internal (MM-40) External (MM-60)

Component Sessional -1 Sessional -2 Assignments End Semester Examination

Weightage 10 10 20 60

CO-PO Matrix Environmental Biotechnology TBBT-504 (II)

Course Outcome PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7 PSO1 PSO2 PSO3

PSO

4

TBBT-504 (II) CO 1. 3 - - - - - 2 2 - 1 1

TBBT-504 (II) CO 2. - - - 2 2 - - - 2 2 -

TBBT-504 (II) CO 3. - - 2 - 3 - - - - 2 -

TBBT-504 (II) CO 4. - 1 - 2 - - 1 - - - 1

Average CO(TBBT-

504 (II) ) 3 1 2 2 2.5 1.5 2 2 1.67 1

Page 142: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc. (H) Biotechnology Programme Code 05

Course Code PBBT-504 (II) Credit 1

Year/Sem 3/5 L-T-P 0-0-2

Course Name Environmental Biotechnology Lab

Objectives of the Course:

1. To develop the practical knowledge and expertise in sample collection, testing and analysis.

2. To study about the environmental pollutant and heavy metals.

3. To study about the methods for the calculation of TDS, BOD, COD, conductivity, pH of

water samples.

4. To study about the methods for bacterial examination of Water by MPN Method and analysis

of data.

PRACTICALS

1. Calculation of Total Dissolved Solids (TDS) of water sample.

2. Calculation of BOD of water sample.

3. Calculation of COD of water sample.

4. Bacterial Examination of Water.

Course Outcomes (COs)

PBBT-504 (II) CO 1.Understanding of environmental pollutants,methods for their estimation

and control.

PBBT-504 (II) CO 2.Practical knowledge of the methods for measurement and their quantitative

as well as qualitative estimation.

PBBT-504 (II) CO 3. Expertise in instrument and sample handling for the preparation and

analysis of samples.

PBBT-504 (II) CO 4.Proficiency in data analysis and perception of samples.

References:

Books

1. PatraJ.K, Das G,Thatoi H.,A Practical Guide to Environmental Biotechnology,Springer

Mode of Evaluation

Internal (MM-25) External (MM-25)

Component Internal End Semester Examination

Weightage 25 25

CO-PO Matrix Environmental Biotechnology Lab PBBT -504 (II)

Course Outcome PO1 PO2 PO3 PO4 PO5 PO6 PO7 PSO1 PSO2 PSO3 PSO4

PBBT-504 (II) CO 1. 2 - - - - - 2 2 1 - -

PBBT-504 (II) CO 2. - 2 1 - 2 - - - - 2 -

PBBT-504 (II)CO 3. - - 2 - - - - - - 1 -

PBBT-504 (II) CO 4. - - - 2 - 1 - - - 1 2

Average CO(PBBT-

504 (II)) 2 2 1.5 2 2 1 2 2 1 1.33 2

Page 143: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc (H) Biotechnology Programme Code 05

Course Code TBBT-504 (III) Credit 4

Year/Sem 3/5 L-T-P 4-0-0

Course Name Medical Microbiology

Objectives of the Course:

1. To provide students intimate knowledge about the microflora, their function and structure.

2. To learn about the pathogen, pathogenicity and microbial disease.

3. To understand the diagnosis, preventions and treatments to cure diseases and infection.

UNIT I (Total Topics- 15 and Hrs- 10))

Introduction: Normal microflora of human body, nosocomial infections, carriers, septic shock,

septicemia, pathogenicity, virulence factors, toxins, biosafety levels.

Morphology, pathogenesis, symptoms, laboratory diagnosis, preventive measures and chemotherapy

of gram positive bacteria: S.aureus, S.pyogenes, B.anthracis, C.perferinges,C.tetani, C.botulinum,

C.diphtheriaeM.tuberculosis, M. leprae.

UNIT II(Total Topics- 15 and Hrs- 10) Morphology, pathogeneis, symptoms, laboratory diagnosis, preventive measures and chemotherapy

caused by gram negative bacteria: E.coli, N. gonorrhoea, N. meningitidis, P.aeruginosa, S. typhi, S.

dysenteriae, Y. pestis, B. abortus, H. influenzae, V. cholerae, M. pneumoniae, T. pallidum M.

pneumoniae, Rickettsiaceae, Chlamydiae.

UNIT III(Total Topics- 12 and Hrs- 10) Diseases caused by viruses- Picornavirus, Orthomyxoviruses, Paramyxoviruses, Rhabdoviruses,

Reoviruses, Pox virus, Herpes virus, Papova virus, Retro viruses (including HIV/AIDS) and

Hepatitis viruses.

UNIT IV(Total Topics- 15 and Hrs- 10) Fungal and Protozoan infections. Dermatophytoses (Trichophyton,

MicrosporunandEpidermophyton) Subcutaneous infection (Sporothrix, Cryptococcus), systemic

infection(Histoplasma, Coccidoides) and opportunistic fungal infections (Candidiasis,

Aspergillosis), Gastrointestinal infections (Amoebiasis, Giardiasis), Blood-borne infections

(Leishmaniasis, Malaria)

Course Outcomes (COs)

TBBT-504 (III) CO 1.Understand the principles, tools and techniques of pathology for diagnostic,

basic research and employment.

TBBT-504 (III) CO 2.Identify the pathological samples to control and prevent the infection, and

diseases.

TBBT-504 (III) CO 3.Provide knowledge in the field of medical microbiology which covers various

healths, social and ecological issues.

References:

Books

1. Brooks G.F. Carroll K.C. Butel J.S. and Morse S. A. Jawetz, Melnick and Adelberg’s Medical

Microbiology McGraw Hill Publication.2007 24th edition

2. Goering R, Dockrell H, Zuckerman M and Wakelin D. Mims’ Medical Microbiology. Elsevier

Page 144: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

2007 4th edition

Mode of Evaluation

Internal (MM-40) External (MM-60)

Component Sessional -1 Sessional -2 Assignments End Semester Examination

Weightage 10 10 20 60

CO-PO Matrix Medical Microbiology TBBT-504 (III)

Course Outcome PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PSO

1

PSO

2

PSO

3

PSO

4

TBBT-504 (III)CO 1. 1 - - 1 - - 1 3 - - 1

TBBT-504 (III)CO 2. - 1 - - 1 - - - - 1 -

TBBT-504 (III) CO 3. - - 1 - - - - - 1 - -

Average CO(TBBT-

504 (III)) 1 1 - 1 1 1 3 1 1 1

Page 145: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc (H) Biotechnology Programme Code 05

Course Code PBBT-504 (III) Credit 1

Year/Sem 3/5 L-T-P 0-0-2

Course Name Medical Microbiology Lab

Objectives of the Course:

1. To learn about the biochemical characteristics of pathogens and their activities.

2. To be expertise in sample handling and testing of pathological samples

3. To be analyzing and interpret the data.

PRACTICALS

1. Identification of pathogenic bacteria (any two) based on cultural, morphological and

biochemical characteristics.

2. Growth curve of a bacterium.

3. To perform antibacterial testing by Kirby-Bauer method.

4. To prepare temporary mounts of Aspergillus and Candida by appropriate staining.

5. Staining methods: Gram’s staining permanent slides showing Acid fast staining, Capsule

staining and spore staining.

Course Outcomes (COs)

PBBT -504 (III) CO1. Acquire technical skill related to various techniques and process utilized in

microbial and pathological studies

PBBT -504 (III) CO2. Identify the pathological samples to control and prevent the infection, and

diseases.

PBBT -504 (III) CO3. Data analysis and perception of testing samples.

References:

Books

1.Brooks GF, Carroll KC,Butel JS and Morse SA. Jawetz, Melnick and Adelberg’s Medical

Microbiology. McGraw Hill Publication. 2007 24th edition

2.Goering R, Dockrell H, Zuckerman M and Wakelin D. Mims’ Medical Microbiology.

Elsevier 2007 4th edition

Mode of Evaluation

Internal (MM-25) External (MM-25)

Component End Semester Examination End Semester Examination

Weightage 25 25

Page 146: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

CO-PO Matrix Medical Microbiology PBBT -504 (III)

Course Outcome PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PSO

1

PSO

2

PSO

3

PSO

4

PBBT -504 (III) CO1. 1 - - 1 - - 1 3 - - 1

PBBT -504 (III)CO2 - 1 - - 1 - - - - 1 -

PBBT -504 (III) CO3 - - 1 - - - - - 1 - -

Average CO (PBBT

504( III)) 1 1 - 1 1 1 3 1 1 1

Page 147: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc (H) Biotechnology Programme Code 05

Course Code TBBT-504 (IV) Credit 4

Year/Sem 3/5 L-T-P 4-0-0

Course Name Plant Anatomy & Physiology

Objectives of the Course:

1. To provide knowledge about the plant histology, physiology, and biochemistry.

2. To learn about the plant metabolism and hormonal activity.

3. To understand the growth parameters, stress and effect of abiotic factors

UNIT I(Total Topics- 10 and Hrs- 10)

Anatomy: The shoot and root apical meristem and its histological organization, simple & complex

permanent tissues, primary structure of shoot & root, secondarygrowth, growth rings, leaf anatomy

(dorsi-ventral and isobilateral leaf).

UNIT II(Total Topics- 15 and Hrs- 10)

Plant water relations and micro & macro nutrients: Plant water relations: Importance of water to

plant life, diffusion, osmosis, plasmolysis, imbibition, guttation, transpiration, stomata & their

mechanism of opening & closing. Micro & macro nutrients: criteria for identification of essentia

lity of nutrients, roles and deficiency systems of nutrients, mechanism of uptake of nutrients,

mechanism of food transport.

UNIT III(Total Topics- 12 and Hrs- 10)

Carbon and nitrogen metabolism : Photosynthesis- Photosynthesis pigments, concept of two photo

systems, photphosphorylation, calvin cycle, CAM plants, photorespiration, compensation point

Nitrogen metabolism- inorganic & molecular nitrogen fixation, nitrate reduction and ammonium

assimilation in plants.

UNIT IV(Total Topics- 12 and Hrs- 10)

Growth & Development: Growth and development: Definitions, phases ofgrowth, growth curve,

growth hormones (auxins, gibberlins, cytokinins, abscisic acid, ethylene).Physiological role and

mode of action, seed dormancy and seed germination, concept of photo-periodism and

vernalization.

Course Outcomes (COs)

TBBT-504 (IV)CO 1. Understand the plant anatomy, physiology and histology.

TBBT-504 (IV)CO 2. Identify the role of abiotic and biotic factors in plant growth and control of

plant health.

TBBT-504 (IV)CO 3. Provide knowledge about the environmental effects on plant growth and

seed health.

References:

Books

1. Dickinson, W.C. Integrative Plant Anatomy. Harcourt Academic Press, USA 2000

2. Fahn, A. Plant Anatomy. Pergmon Press, USA and UK 1974.

3. Salisbury, F.B. and Ross, C.W. Plant Physiology, Wadsworth Publishing Co. Ltd. 1991

4. Taiz, L. and Zeiger, E. Plant Physiology, Sinauer Associates Inc .MA, USA 20064th edition

5. Mauseth, J.D. Plant Anatomy. The Benjammin/Cummings Publisher, USA 1988

Page 148: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Mode of Evaluation

Internal (MM-40) External (MM-60)

Component Sessional -1 Sessional -2 Assignments End Semester Examination

Weightage 10 10 20 60

CO-PO Matrix Plant Anatomy and Physiology TBBT -504 (IV)

Course Outcome PO1 PO2 PO3 PO4 PO5 PO6 PO7 PSO1 PSO2 PSO3 PSO4

TBBT-504 (IV)CO 1. 3 - - - - - 1 3 - - 1

TBBT-504 (IV)CO 2. - 1 - - 1 - - - - 1 -

TBBT-504 (IV)CO 3. - - - 1 - - - - 1 - -

Average CO (TBBT-

504 (IV) 2 1 - 1 1 1 3 1 1 1

Page 149: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc (H) Biotechnology Programme Code 05

Course Code PBBT-504 (IV) Credit 1

Year/Sem 3/5 L-T-P 0-0-2

Course Name Plant Anatomy & Physiology Lab

Objectives of the Course:

1. To learn about the plant anatomy, ultrastructure, biochemical pathways.

2. To be expertise in plant sample handling, testing and analyzing.

PRACTICALS

1. Preparation of stained mounts of anatomy ofmonocot and dicot’s root, stem & leaf.

2. Demonstration of plasmolysis by Tradescantia leaf peel.

3. Study of opening & closing of stomata

4. Demonstration of guttation on leaf tipsof grass and garden nasturtium.

5. Separation of photosynthetic pigments by paper chromatography.

6. Demonstration of aerobic respiration.

7. Preparation of root nodules from a leguminous plant.

Course Outcomes (COs)

PBBT 504 (IV) CO1. Acquire technical skill related to various techniques and process utilized in

plant physiology study.

PBBT 504 (IV) CO2. Expertise to identify the metabolic pathways and products of plants.

PBBT 504 (IV) CO3. Data analysis and perception of testing samples.

References:

Books

1. Taiz, L. and Zeiger, E. Plant Physiology, Sinauer Associates Inc .MA, USA 2006 4th edition

2. Mauseth, J.D. Plant Anatomy. The Benjammin/Cummings Publisher, USA. 1988

Mode of Evaluation

Internal (MM-25) External (MM-25)

Component Internal End Semester Examination

Weightage 25 25

CO-PO Matrix Plant Anatomy and Physiology PBBT -504 (IV)

Course Outcome PO1 PO2 PO3 PO4 PO5 PO6 PO7 PSO1 PSO2 PSO3 PSO4

PBBT 504 (IV) CO1. 3 - - - - - 2 3 - - 1

PBBT 504 (IV) CO2. 1 1 - - 1 - - - - 1 -

PBBT 504 (IV) CO3. - - 1 - - - - - 1 - -

Average CO (PBBT

-504 (IV) 2 1 1 - 1 2 3 1 1 1

Page 150: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc. (H) Biotechnology Programme Code 05

Course Code ADP-505 Credit 1

Year/Sem 3/5 L-T-P 0-0-2

Course Name Presentation(Industrial training)

Objectives of the Course:

1.To acquire exposure of students pertaining to industrial procedures and protocols.

2.To develop skills, safety measures through Goods Laboratory Practices and guidelines in

industry.

3.To acquire good presentation and communication skills.

Guidelines: • Students will have to submit a report and give a presentation on industrial training

done in the industry or in research organization / laboratory, for about 3-4 weeks between 4th and 5th semester.

• Evaluation will be done on the basis of presentation and submission of report.

Course Outcomes (COs)

ADP-505 CO1. Acquire exposure pertaining to working and application of industrial processes,

analytical techniques, instrumentation and recent technological advancement

ADP-505 CO2. Identify goods laboratory practices, safety guidelines and methodology of data

compilation.

ADP-505 CO3. Enhance presentation, communication and report writing skills

Mode of Evaluation

External (MM-100)

Component Sessional

Weightage 100

CO-PO Matrix Presentation(Industrial training)ADP-505

Course Outcome PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PSO

1

PSO

2

PSO

3

PSO

4

ADP-505 CO 1. 1 1 2 - 1 - - 1 1 2 -

ADP-505 CO 2. 1 - 1 - 1 - - - 1 2 -

ADP-505CO 3. - - - - 3 - - - - - -

Average CO(ADP-

505) 1 1 1.5 - 1.6 - - 1 1 2 -

Page 151: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc. (H) Biotechnology Programme Code 05

Course Code ADP-506 Credit 1

Year/Sem 3/5 L-T-P 2-0-0

Course Name Reasoning Skills

Objectives of the Course:

1. To check if candidates have the adequate problem solving and analytical skills needed in an

organization. The person should have enough problem solving skills to listen to the problem

and immediately identify the proper solution.

2. To gamut the skills which facilitate them to enhance their employability quotient and overall

personality development

3. To understand these skills and enable students to manage the placement challenges more

effectively.

UNIT I (Total Topics- 2 and Hrs- 5 )

Series Completion (Basic Concepts and Problems Solving According to Difficulty

Level)Number Series, Alphabet Series, Alpha-Numeric Series; Coding Decoding (Basic

Concepts and Problems Solving According to Difficulty Level) Letter Coding, Direct Letter

Coding, Number/Symbol Coding, Matrix Coding, Substitution, Deciphering Message

Word Code\s, Deciphering Number and Symbol Codes for Messages

UNIT II (Total Topics -3 and Hrs-5)

Blood Relations (Basic Concepts and Problems Solving According To Difficulty Level)

Deciphering Jumbled Up Descriptions, Relation Puzzles, Coding Relations.Direction Sense Test

(Basic Concepts and Problems Solving According To Difficulty Level) Problems based on

Distance and Direction, Problems based on Angles,Alphabet Test (Basic Concepts and Problems

Solving According To Difficulty Level) Alphabetical Quibble, Letter Word Problems, Rule

Detection.

UNIT- III (Total Topics -5 and Hrs-9)

Alpha Numeric Sequence Puzzles (Basic Concepts and Problems Solving According To

Difficulty Level)Number, Ranking and Time Sequence Test (Basic Concepts and Problems

Solving According To Difficulty),Problems Based on Seating/Standing Positions, Problems

based on Numbers, Problems based on Days ,Venn Diagram(Basic Concepts and Problems

Solving According To Difficulty Level),Syllogism (Basic Concepts and Problems Solving

According To Difficulty Level),Inequalities (Basic Concepts and Problems Solving According

To Difficulty Level):Direct Inequalities, Coded Inequalities.

UNIT-IV (Total Topics -4 and Hrs-10)

Seating Arrangement (Basic Concepts and Problems Solving According To Difficulty

Level),Circular Arrangement, Linear Arrangement (Facing North and Facing South),

Rectangular Arrangement, Mix Seating Arrangement (Blood Relation with Seating

Arrangement) , Analytical Reasoning (Puzzle Test) (Basic Concepts and Problems Solving

According To Difficulty Level),Classification Type Questions, Comparison Type

Question,Sequential Order Of Things, Selection Based On Given Conditions, Family Based

Puzzles,Analogy and Classification (Basic Concepts and Problems Solving According To

Difficulty Level),Problems based on Numbers, Problems based on Alphabets,Data

Sufficiency(Basic Concepts and Problems Solving According To Difficulty Level),Data

Page 152: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Sufficiency related to Seating Arrangement, Direction Sense, Blood Relation, Coding Decoding

etc.

Course Outcomes (COs)

ADP-506 CO1. Solve campus placements and other competition aptitude papers covering

Logical Reasoning

ADP-506 CO2. Formulate the problem quantitatively and use appropriate arithmetical, and/or

statistical methods to solve the problem

ADP-506 CO3. Interpret quantitative information (i.e., formulas, graphs, tables, models, and

schematics) and draw implications from them.

ADP-506 CO4. Thinking critically and applying basic numerical reasoning skills to interpret

data, draw conclusions, and solve problems.

Reference Books: 1. Aggarwal R.S ,Quantitative Aptitude ,S. Chand Publisher,2017 2. Aggarwal R.S ,A Modern Approach to Logical Reasoning ,S. Chand Publisher,2017 3. Aggarwal R.S ,A Modern Approach to Verbal & Non-Verbal Reasoning , S. Chand

Publisher 2017 4. Sharma Arun,Quantitative Aptitude , MHE Publisher, 2018 8th edition

Mode of Evaluation

Internal MM -50

Component Sessional

Weightage 50

CO-PO Matrix Reasoning Skills ADP-506

Course Outcome PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PSO

1

PSO

2

PSO

3

PSO

4

ADP-506 CO 1. - - - - - - 2 - - - -

ADP-506 CO 2. - - - - - - - - - - -

ADP-506 CO 3. - - - - - - - - - - -

ADP-506 CO 4. - - - - - - - - - - -

Average CO(ADP-

506) - - - - - - 2 - - - -

Page 153: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

SEMESTER-VI

Page 154: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc. (H) Biotechnology Programme Code 05

Course Code TBBT-601 Credit 4

Year/Sem 3/6 L-T-P 4-0-0

Course Name Bio Analytical Tools & Techniques

Objectives of the Course:

1. To provide scientific knowledge related to various techniques associated with biotechnology.

2. To impart laboratory skills for handling analytical tools in industry and research institution.

3. To give the scientific knowledge regarding safety regulations for handling of instruments in the

laboratory and industry.

4. To demonstrate the operating procedures associated with upstream and downstream process

like chromatography, electrophoresis, centrifugation, etc.

UNIT I (Total Topics- 7 and Hrs- 10)

Light microscopy, Bright & Dark Field microscopy, Phase Contrast microscopy, Fluorescence and

Electron microscopy (TEM and SEM)

UNIT II (Total Topics -14 and Hrs-10)

Spectroscopy: Principle and laws of absorption, fluorimetry, colorimetry, spectrophotometry

(visible, UV, infra-red). Basics of X-Ray Diffraction.

Centrifugation: Basic Principle & Instrumentation, Factors affecting sedimentation velocity,

standard sedimentation coefficient; Basics of Rate-Zonal centrifugation & Ultracentrifugation.

UNIT- III (Total Topics -10 and Hrs-10)

Chromatography: Principle of chromatography. Paper chromatography, thin layer chromatography,

column chromatography: silica and gel filtration, affinity and ion exchange chromatography, gas

chromatography, HPLC.

UNIT-IV (Total Topics -9 and Hrs-10)

Electrophoresis: Introduction to electrophoresis. Paper electrophoresis, Gel electrophoresis,

immune-electrophoresis, isoelectric focusing,

Introduction to Biosensors and Nanotechnology with their applications.

Course Outcomes (COs)

TBBT-601 CO1. Development of scientific and analytical knowledge regarding various tools and

techniques in the field of applied science.

TBBT-601 CO2. Apply modern techniques and their statistical knowledge for solving various

scientific problems in industry and research institution

TBBT-601CO3. Demonstrating the scientific knowledge regarding safety regulations for handling

of scientific instruments in the laboratory

TBBT-601 CO4. Acquire knowledge related to the techniques associated with upstream and

downstream process like chromatography, electrophoresis, centrifugation, etc

References:

Books

1.Wilson & Walker, Principles & Techniques of Biochemistry & Molecular Biology, Cambridge

University Press.

2.Upadhyay & Upadhyay, Biophysical Chemistry: Principles & Techniques, Himalaya Publishing

House.

Mode of Evaluation

Page 155: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Internal (MM-40) External (MM-60)

Component Sessional -1 Sessional -2 Assignments End Semester Examination

Weightage 10 10 20 60

CO-PO Matrix Bio Analytical Tools & TechniquesTBBT-601

Course Outcome PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7 PSO1 PSO2 PSO3 PSO4

TBBT-601CO 1. 3 1 2 2 2 - - 3 3 2 1

TBBT-601CO 2. 3 2 2 1 1 - - 3 3 1 -

TBBT-601CO 3. 3 - 1 1 1 - - 2 2 2 1

TBBT-601CO 4. 3 2 1 1 2 - - 3 2 2 1

Average CO(TBBT-

601) 3 1.6 1.5 1.2 1.5 - - 2.75 2.5 1.75 1

Page 156: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc. (H) Biotechnology Programme Code 05

Course Code PBBT-601 Credit 2

Year/Sem 3/6 L-T-P 0-0-4

Course Name Bio Analytical Tools & Techniques Lab

Objectives of the Course:

1. Development of scientific and analytical knowledge regarding various tools andtechniques in

the field of applied science.

2. Apply modern techniques and their statistical knowledge for solving variousscientific

problems in industry and research institution

3. Development of the scientific knowledge regarding safety regulations for handling

ofscientific instruments in Industry.

4. Demonstrating the principles and working of bio-analytical techniques associatedwith

upstream and downstream process like chromatography, electrophoresis,centrifugation, etc

PRACTICALS

1. Native gel electrophoresis of proteins

2. SDS-polyacrylamide slab gel electrophoresis of proteins.

3. Separation of amino acids by paper chromatography.

4. To identify biomolecules in a given sample by TLC.

5. To verify the validity of Beer’s law.

6. Determination of λmax of the given sample.

7. Demonstration of Western blotting.

8. Demonstration of HPLC.

9. Demonstration of Density-Gradient centrifugation.

Course Outcomes (COs)

PBBT-601 CO 1.Exhibit working and operating knowledge regarding various tools and

techniques in the field of applied science.

PBBT-601 CO 2. Apply modern techniques and their statistical knowledge for solving various

scientific problems in industry and research institution

PBBT-601 CO 3. Development of scientific knowledge regarding safety regulations for

handling of scientific instruments in Industry.

PBBT-601 CO 4. Demonstrating the working of bio-analytical techniques associated like HPLC,

SDS PAGE, centrifugation, etc

References:

Books

1.Wilson & Walker, Principles & Techniques of Biochemistry & Molecular Biology, Cambridge

University Press.

2.Upadhyay & Upadhyay, Biophysical Chemistry: Principles & Techniques, Himalaya

Publishing House.

Page 157: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Mode of Evaluation

Internal (MM-25) External (MM-25)

Component

Internal

End Semester Examination

Weightage 25 25

CO-PO Matrix Bio Analytical Tools & Techniques Lab PBBT-601

Course Outcome PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PSO

1

PSO

2

PSO

3

PSO

4

PBBT-601 CO 1. 3 1 3 1 1 - - 1 3 2 1

PBBT-601 CO 2. 3 2 2 - 1 - - 1 3 - 1

PBBT-601 CO 3. 2 - 2 - 1 - - 2 2 2 -

PBBT-601 CO 4. 3 2 3 1 2 - - 3 2 2 1

Average CO(PBBT-

601) 2.7 1.6 2.5 1 1.2 - - 1.75 2.5 2 1

Page 158: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc. (H) Biotechnology Programme Code 05

Course Code TBBT-602 Credit 4

Year/Sem 3/6 L-T-P 4-0-0

Course Name Genomics and Proteomics

Objectives of the Course:

1. To provide sound knowledge pertaining to different methods, tools and techniques utilized to

study structure and function of genomics, proteomics and transcriptomics.

2. To study about basic concepts and methods of genome analysis/ software.

3. To study about gene modification, expression analysis and their storage of data by using bio-

informatics tools.

4. To study about the proteomics and various techniques used for protein isolation, separation,

protein sequencing, and software used for the conservation and engineering of genome.

UNIT I (Total Topics- 12 and Hrs- 10)

Introduction to Genomics, Structural and Functional genomics, Genome Mapping, DNA

sequencing methods –Maxam & Gilbert and Sanger’s method. Pyrosequencing, Genome

Sequencing: Shotgun & Hierarchical (clone contig) methods, Computer tools for sequencing

projects: Genome sequence assembly software.

UNIT II (Total Topics -10 and Hrs-10)

Transcriptomics: Gene expression analysis, Identification of Biomarkers, techniques for studying

the transcriptome: Microarray, Substractive Hybridization, Northern Blotting, SAGE, cDNA

sequencing.

UNIT- III (Total Topics -8 and Hrs-10)

Introduction to Proteomics: Isolation of Proteins, Seperation of Proteins: Isoelectric Focussing,

2D-Gel electrophoresis, Protein Protein Interactions, Protein DNA Interaction, Native PAGE

UNIT-IV (Total Topics -8 and Hrs-10)

Mass spectrometry based methods for protein Sequencing and identification. Denovo sequencing

using mass spectrometric data. Protein Sequencing – Edman degradation. Introduction to

Comparative Genomics, Metagenomics.

Course Outcomes (COs)

TBBT-602 CO 1.Punitive knowledge of the biological systems information and the explanation

of the key concepts Omics technologies-genomics, transcriptomics and proteomics.

TBBT-602 CO 2. Comprehend the range of molecular biology techniques for DNA or genome

profiling, sequencing, assembly, annotation, summaries and interpretation of the outcome.

TBBT-602 CO 3. Knowledge of bioinformatics tools for the genomic data storage and analysis

of the outgoing research in the area of genomic and proteomic studies.

TBBT-602 CO 4. Erudition skills on software for data analysis relevant to forensic,

conservation, quantitative and evolutionary genetics. References:

Books

1.Sambrook and Russell, Molecular Cloning: A Laboratory Manual, Cold Spring Harbor

Laboratory Press, Vol. I to III, 1989 3rd Edition.

2.Primrose, S.B. and Twyman, R.M., Principles of Gene Manipulation, Old. Blackwell Science

2001 6th edition.

Page 159: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

3.Snustad, D. P. and Simmons, M..J., Principles of Genetics, John Wiley and Sons Inc., 20095th

Edition

4.Pevsner, J., Bioinformatics and Functional Genomics, John Wiley & Sons, 2009 2nd edition

Mode of Evaluation

Internal (MM-40) External (MM-60)

Component Sessional -1 Sessional -2 Assignments End Semester Examination

Weightage 10 10 20 60

CO-PO Matrix Genomics and ProteomicsTBBT-602

Course Outcome PO1 PO2 PO3 PO4 PO5 PO6 PO7 PSO1 PSO2 PSO3 PSO4

TBBT-602 CO 1. 3 - - - - - 2 2 - 1 -

TBBT-602 CO 2. - 2 - - 2 - - - 2 - -

TBBT-602 CO 3. - 2 1 - 3 - - - - 2 -

TBBT-602 CO 4. - - - 2 - - - - - - 1

Average

CO(TBBT-602) 3 2 1 2 2.5 2 2 2 1.5 1

Page 160: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc. (H) Biotechnology Programme Code 05

Course Code PBBT-602 Credit 2

Year/Sem 3/6 L-T-P 0-0-4

Course Name Genomics And Proteomics Lab

Objectives of the Course:

1. To study about the bio-informatics tools and databases.

2. To learn the about the methods of protein and genome estimation and separation.

3. To be expertise in running of programs, bio-informatics tools, software for the genomic data

analysis.

4. To develop practical skill in handling of biological samples, pedigree chart, analysis of

inheritance pattern.

PRACTICALS

1. Use of SNP databases at NCBI and other sites

2. Use of OMIM database

3. Detection of Open Reading Frames using ORF Finder

4. Proteomics 2D PAGE database

5. Softwares for Protein localization.

6. Hydropathy plots

7. PAGE

Course Outcomes (COs)

PBBT-602 CO1. Practical knowledge of molecular techniques for genome collection and data

analysis.

PBBT-602 CO2. Expertise in instrument handling and bio-informatics software for the analysis

of biological samples.

PBBT-602 CO3. Expand proficiency in data analysis and perception of samples to solve

problems (multidisciplinary) contexts related to the field of study.

PBBT-602 CO4. Design of experiments and manage bibliography and IT resources related to

biochemistry, molecular biology or biomedicine about the scientific or business context. References:

Books

1.Keith Geoffrey ,Rickards and Jones R. N.,Practical Genetics, Wiley

2.BaharTaneri,EsraAsilmazV,PembeSavas, SeniyeTargen,YagmurEsemen,Human Genetics and

Genomics: A Practical Guide,

Mode of Evaluation

Internal (MM-25) External (MM-25)

Component Internal End Semester Examination

Weightage 25 25

Page 161: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

CO-PO Matrix Genomics And Proteomics LabPBBT-602

Course Outcome PO1 PO2 PO3 PO4 PO5 PO6 PO7 PSO1 PSO2 PSO3 PSO4

PBBT-602 CO 1. 2 - - - - - 2 2 1 - -

PBBT-602 CO 2. - 1 1 - 2 - - - - 2 -

PBBT-602 CO 3. - - 2 - - - - - - - -

PBBT-602 CO 4. - - - 2 - 1 - - - - 2

Average

CO(PBBT-602) 2 1 1.5 2 2 1 2 2 1 2 2

Page 162: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc. (H) Biotechnology Programme Code 05

Course Code TBBT-603 (I) Credit 4

Year/Sem 3/6 L-T-P 4-0-0

Course Name Plant Biotechnology

Objectives of the Course:

1. To provide a vast knowledge regarding plant growth technologies &modifications for a better

future in agriculture with help of analytical tools and technologies.

2. To acknowledge the various methods of Tissue culture, its importance & application, study

of usage of the certain micro flora aiding in the plants growth with help of techniques, data

interpretation, and implementing the acquired knowledge in biotechnological, medical and

agricultural fields with a lifelong understanding.

3. To widen the knowledge acquired by handling of classical and modern plant

biotechnology processes, including breeding of healthy plants with improved characteristics

and plants for biomolecules production to solve productivity problems.

4. Toimpart the understanding of the basic principles of the plant sciences on the molecular

level as well as the integration to provide healthy plants in for food, feed and health

applications worldwide as well encourage students to choose a desired career

UNIT I (Total Topics- 16 and Hrs- 10)

Introduction, Media and Growth supplements, Cryo and organogenic differentiation, Types of

culture: Seed, Embryo, Callus, Organs, Cell and Protoplast culture. Micropopagation Axillary

bud proliferation, Meristem and shoot tip culture, bud culture, organogenesis, embryogenesis,

advantages and disadvantages of micropropagation.

UNIT II (Total Topics -11 and Hrs-10)

In vitro haploid production Androgenic methods: Anther culture, Microspore culture

andogenesis Significance and use of haploids, Ploidy level and chromosome doubling,

diplodization, Gynogenic haploids, factors effecting gynogenesis, chromosome elimination

techniques for production of haploids in cereals.

UNIT- III (Total Topics -10 and Hrs-10)

Protoplast Isolation and fusion Methods of protoplast isolation, Protoplast development, Somatic

hybridization, identifiation and selection of hybrid cells, Cybrids, Potential of somatic

hybridization limitations. Somaclonal variation: methods, applications and disadvantages.

UNIT-IV (Total Topics -8 and Hrs-10)

Plant Growth Promoting bacteria. Nitrogen fixation, Nitrogenase, Hydrogenase, Nodulation,

Biocontrol of pathogens, Growth promotion by free-living bacteria

Course Outcomes (COs)

TBBT-603 (I) CO 1.Understand the concept and principle of micropropagation technique and

identify designing of culture medium types of culture and application in agriculture and

biological research with emphasis on conservation studies.

TBBT-603 (I) CO 2.Identify the potential of micropropagation to produce haploids and

significance of involved techniques such as chromosome elimination with utility of such plants

in agriculture.

TBBT-603 (I) CO 3. Demonstrate understanding of principle involved and technique adopted

Page 163: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

for protoplast isolation and fusion and applicability to produce hybrid plants.

TBBT-603 (I) CO 4.Exhibit knowledge about plant growth promoting bacteria free living

bacteria their impact on plants and environment and interpret applicability of hydroponics in

tissue culture studies. References:

Books

1. Bhojwani, S.S. and Razdan, Plant Tissue Culture and Practice, Elsevier, 2004.

2. Raven, P.H., Johnson, G.B., Losos, J.B. and Singer, S.R., Biology, Tata MC Graw Hill, 2005.

3. Reinert, J. and Bajaj, Y.P.S., Applied and Fundamental Aspects of Plant Cell, Tissue and

Organ Culture., Narosa Publishing House,1997

4. Chawla, H. S., Plant Biotechnology, S. Chand Publications.

5. Slater, A., Scott, N.W. and Fowler, M. R., Plant Biotechnology: The Genetic Manipulation of

Plants, Oxford University Press, 2008.

CO-PO Matrix Plant Biotechnology TBBT-603 (I)

Course Outcome PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PSO

1

PSO

2

PSO

3

PSO

4

TBBT-603 (I) CO 1. 2 - - - - - - 2 - 3 1

TBBT-603 (I) CO 2. - 2 - 1 - - 1 1 - 2 1

TBBT-603 (I) CO 3. - - - - - - - 2 - 2 -

TBBT-603 (I) CO 4. - 1 3 - - - - 1 - - -

Average CO(TBBT-603

(I) ) 2 1 3 1.8 - - 1 1.5 - 2.3 -

Mode of Evaluation

Internal (MM-40) External (MM-60)

Component Sessional -1 Sessional -2 Assignments End Semester Examination

Weightage 10 10 20 60

Page 164: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc. (H) Biotechnology Programme Code 05

Course Code PBBT-603 (I) Credit 1

Year/Sem 3/6 L-T-P 0-0-2

Course Name Plant Biotechnology Lab

Objectives of the Course:

1. To encompass tools&methods in tissue culture which are used in crop improvement.

2. To explain the concepts, principles and usage of the acquired knowledge of tissue culture

techniques in laboratory.

3. To acknowledge the use of media,growth hormones for growth of plants in vitro.

4. To handle analytical tools,learn safety measures,specimen handling and inculcating the

requirements for setup of a plant tissue culture laboratory PRACTICALS

1. Preparation of simple growth nutrient (knop’s medium), full strength, half strength, solid and

liquid.

2. Preparation of complex nutrient medium (Murashige& Skoog’s medium / White / Gamborg)

3. Sterilization of explant for culture.

4. Significance of growth hormones in culture medium.

5. To demonstrate various steps of micropropagation

Course Outcomes (COs)

PBBT-603 (I) CO 1.Conduct tissue culture experiments for conservation and mass propagation of

plant species and recognize significance of micropropagation in crop improvement

PBBT-603 (I) CO 2.Acknowledgement of a ideal plant tissue culture laboratory setup, its

components as well learning the handling of plant specimens and studying their core characteristics

for health, medicine, Agriculture etc.

PBBT-603 (I) CO 3. Identify concepts and principles of various tools, techniques, utilized in plant

biotechnology laboratory alongwith data analysis and interpretation.

PBBT-603 (I) CO 4.Comprehend various culture techniques, their application, recording

observations and analysis References: Books

1.OlufGamborg,Gregory C. Phillips , Plant Cell, Tissue and Organ Culture: Fundamental

Methods (Springer Lab Manuals), Springer, 2013 1995 edition

Mode of Evaluation

Internal (MM-25) External (MM-25)

Component Internal End Semester Examination

Weightage 25 25

Page 165: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

CO-PO Matrix Plant Biotechnology Lab PBBT-603 (I)

Course Outcome PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PSO

1

PSO

2

PSO

3

PSO

4

PBBT-603 (I) CO 1. 2 - - 1 1 - - - 2 3 -

PBBT-603 (I) CO 2. - - 2 - 1 - 1 1 - 2 -

PBBT-603 (I) CO 3. - - 3 1 - - 1 - 2 - 1

PBBT-603 (I) CO 4. - 1 2 - 2 - - - 2 - -

Average CO(PBBT-603

(I) ) 2 1 2.3 1.4 2 - 1 1 2 1.7 1.7

Page 166: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc (H) Biotechnology Programme Code 05

Course Code TBBT-603 (II) Credit 4

Year/Sem 3/6 L-T-P 4-0-0

Course Name Animal Diversity - I

Objectives of the Course:

1.To learn the applied knowledge of animal diversity,classification along with their unique

fundamental morphologicalfeature in animal kingdom.

2.To make students learn the application of phylogeny in the field of modern biological science.

3.To familiarize the students through scientific enquiry into the nature of mechanical, physical, and

biochemical functions of animal system.

4.To provides theoretical and practical designing of recent kingdom classification techniques and

their conducting experiments in laboratories.

UNIT I (Total Topics- 10 and Hrs-10)

Outline of classification of Non- Chordates upto subclasses. Coelomata, Acoelomata, Symmetries,

Deutrostomes, Protostomes. Protozoa: Locomotion, Reproduction, evolution of Sex, General features

of Paramoeciumand Plasmodium. Pathogenic protozoans. Porifera: General characters, outline of

Classification; skeleton, Canal System.

UNIT II (Total Topics -10 and Hrs-10)

Coelenterata: General Characters, Outline of classifications Polymorphism, Various types of stinging

cells; Metagenesis, coral reefs and their formation. Platyhelminthes- General Characters; Outline of

classification; Pathogenic flatworms: Parasitic adaptations. Aschelminthes: General features, Outline

of classification, Pathogenic roundworms and their vectors in relation to man: Parasite adaptation.

UNIT- III (Total Topics -10 and Hrs-10)

Annelida: - General features, Outline of classification, Coelom: Metameric segmentation, General

features of Earthworm, Vermicomposting. Arthropoda: General Features, Outline of Classification;

Larval forms of crustacean, Respiration in Arthropoda; Metamorphosis in insects; Social insects;

Insect vectors of diseases; Apiculture, Sericulture.

UNIT-IV (Total Topics -10 and Hrs-10)

Mollusca: general features, Outline of classification, Shell Diversity; Torsion in gastropoda,

Echinodermata: General features, Outline of Classification Larval forms. Hemichordata: Phylogeny:

Affinities of Balanoglossus.

Course Outcomes (COs)

TBBT-603 (II) CO1.Applied knowledge of morphological, anatomiccharacterization along with their

fundamental values in the mammalian body at the cellular level and system level.

TBBT-603 (II) CO2.Understand the designing of recent physiological techniques with related to

animalclassification system.

TBBT-603 (II) CO3.Knowledge and appreciation of mammalian physiology and understand the

functionalof cardio, respiratory, renal, digestive and endocrine systems.

TBBT-603 (II) CO4.Practicing group learning through scientific inquiry into the nature of

mechanical, physical, and biochemical functions of different systems of animal.

References:

Books

Page 167: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

1.Barnes, R.S.K., Calow, P., Olive, P.J.W., Golding, D.W. and J.I., Spicer The Invertebrates: A New

Synthesis Blackwell Science. 2002 3rd edition

2.Barrington, E.J.W. Invertebrate Structure and Functions. E.L.B.S. and Nelson 1979 2nd edition

3.Boradale, L.A. and Potts, E.A. Invertebrates: A Manual for the use of Students. Asia Publishing

Mode of Evaluation

Internal (MM-40) External (MM-60)

Component Sessional -1 Sessional -2 Assignments End Semester Examination

Weightage 10 10 20 60

CO-PO Matrix Animal Diversity - I TBBT-603 (II)

Course Outcome PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PSO

1

PSO

2

PSO

3

PSO

4

TBBT-603 (II) CO 1. 2 2 - 1 - 1 3 2 - 2 2

TBBT-603 (II) CO 2. - - 2 - 2 1 2 - -

TBBT-603 (II) CO 3. 2 2 - - 2 2 2 -

TBBT-603 (II) CO 4. - 2 2 - 2 1 - - 2 2

Average CO(TBBT-

603 (II)) 2.0 2.0 2.0 1.0 2.0 1.0 2.5 2.00 2.00 2.00 2.00

Page 168: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc (H) Biotechnology Programme Code 05

Course Code PBBT-603 (II) Credit 1

Year/Sem 3/6 L-T-P 0-0-2

Course Name Animal Diversity - I Lab

Objectives of the Course:

1. To enable the students to acquire physiology based experiments of modern biomedical science.

2. To familiarize the students through scientific enquiry into the nature of mechanical, physical,and

biochemical functions of diversity of animal system.

3. To impart practical knowledge of fundamental principles in mammalian physiology.

4. To develop the theoretical based practical designing of recent physiological techniques and their

conducting experiments in laboratories.

PRACTICALS

Identification and Classification of Any these of the following –

1. Porifera: Scypha, , Leucosolenia, Euspongia, Hylonema, EuplectellaCnidaria: Medrepora,

Millepora, Physalia, Porpita, Valella, Aurelia, Metridium

2. Platyhelminthes: Taenia, Fasciola, Aschelminthes: Ascaris, EnterobiusAnnelida: Pheretima,

Hirudinaria, Chaetopterus, Nereis, Aphrodite Arthropoda: Julus, Scolopendra, Peripatus, ,

Limulus, Lepisma, Dragonfly, Musca, AchetaMollusca: Pila, Unio, Mytilus, Loligo, Sepia,

Octopus, SolenEchinodermata: Asterias, Echinus, Holothuria, AstrophytonHemichordata:

Balanoglossus

3. Identification of slides with two points of identification. Amoeba, Paramoecium, Ceratium,

Plasmodium, Opalina, L.S. Sponge, Spicule of sponges, L.S. Hydra, Obelia, Bougainvillia,

Larvae ofFasciola, Seta of Earthworm, Radula

4. Ecological Note – On any of the specimens in Exercise No 1

5. Models of dissection of Earthworm, Cockroach

6. Earthworm: Digestive, Nervous System,

7. Cockroach: Digestive Reproductive, Nervous System

Course Outcomes (COs)

PBBT-603 (II) CO1.Demonstrate the experimental techniques related to animal diversity at the

cellular level.

PBBT-603 (II) CO2.Understand the designing of recent physiological and morphological techniques

and their conducting experiments in laboratories.

PBBT-603 (II) CO3.Generate and interpret the test hypotheses, analyze the data by using modern

methods.

PBBT-603(II) CO4.Practicing group learning through scientific inquiry into the nature of

mechanical, physical,and biochemical functions of different locomotion, reproductive system of

animals.

References:

Books 1. Boradale, L.A. and Potts, E.A Invertebrates: A Manual for the use of Students. Asia Publishing

Home.1961

Mode of Evaluation

Page 169: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Internal (MM-25) External (MM-25)

Component Internal End Semester Examination

Weightage 25 25

CO-PO Matrix Animal Diversity – ILab PBBT-603 (II)

Course Outcome PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PSO

1

PSO

2

PSO

3

PSO

4

PBBT-603 (II) CO 1. 2 2 - 1 3 1 3 2 3 2 2

PBBT-603 (II) CO 2. 1 - 2 - 2 1 - 2 2 - -

PBBT-603 (II) CO 3. 2 2 - - - - 2 2 - 1 -

PBBT-603 (II) CO 4. - - 2 - 2 2 - - - - 2

Average CO (PBBT-

603 (II) 1.6 2.0 2.0 1.0 2.3 1.3 2.0 2.0 2.5 1.5 2.0

Page 170: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc (H) Biotechnology Programme Code 05

Course Code TBBT-603 (III) Credit 4

Year/Sem 3/6 L-T-P 4-0-0

Course Name Plant Diversity-I

Objectives of the Course:

1. To provide fundamental aspects of plant physiology with especial focus on plant mineral

translocation related to nutrition.

2. To learn basic concept of life cyclebelonging to various classes.

3. To understand the basics metabolic studies and assesses dormancy and seed germination in

plants.

4. To learn about the growth, development, disease and economic importance in plants.

UNIT I (Total Topics- 10 and Hrs-10)

Algae: General character, classification and economic importance. Life histories of algae belonging

to various classes: Chlorophyceae – Volvox, OedogoniumXanthophyceae –VaucheriaPhaeophyceae

– EctocarpusRhodophyceae-Polysiphonia

UNIT II (Total Topics -10 and Hrs-10)

Fungi: General characters, classification & economic importance. Life histories of Fungi

:Mastigomycontina- PhytophthoraZygomycotina- MucorAscomycotina-

Saccharomyces.Basidomycotina-Agaricus.Deutromycotina-Colletotrichum

UNIT- III (Total Topics -7 and Hrs-10)

Lichens: Classification, general structure, reproduction and economic importance. Plant diseases:+-

Casual organism, symptoms and control of plant diseases.Rust & Smut of Wheat.White rust of

Crucifers.Late blight of Potato.Red rot of Sugarcane.Citrus Canker

UNIT-IV (Total Topics -8 and Hrs-10)

Bryophytes: General characters, classification & economic impotence.Life histories of

Marchantia.Funaria.

Course Outcomes (COs)

TBBT-603 (III)CO 1.Impart an insight into the various plant water relations and mineral nutrition

in plants.

TBBT-603 (III) CO 2.Depict ability to identify and estimate various photosynthetic pigments

found in leaf and its application in botanical studies.

TBBT-603 (III)CO 3 .Understand the mechanism of various metabolic processes in plants and

access economic importance of different lower plant families.

TBBT-603 (III) CO 4.Equip students with skills and techniques related to plant pathology so that

they can design their own experiments, gain knowledge and make good career in this field.

References:

Books

1. Agrios, G.N. Plant Pathology, , Academic Press, U.K. 1997 4th edition.

2. Alexopoulos, C.J., Mims, C.W. and Blackwell, M. Introductory Mycology, John Wiley and Sons

(Asia) Singapore 1996 4th edition.

3. Bold, H.C. & Wayne, M.J. Introduction to Algae.1996 2nd edition

4. Kumar, H.D. Introductory Phycology. Aff. East-West Press Pvt Ltd., Delhi. 1999

5. Lee, R.E. Phycology, Fourth Edition, Cambridge University Press, USA. 2008

Page 171: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

6. SambamurtyA Textbook of Bryophytes, Pteridophytes, Gymnosperms and Paleobotany, IK

International Publishers. 2008

Mode of Evaluation

Internal (MM-40) External (MM-60)

Component Sessional -1 Sessional -2 Assignments End Semester Examination

Weightage 10 10 20 60

CO-PO Matrix Plant Diversity-I TBBT-603 (III)

Course Outcome PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PSO

1

PSO

2

PSO

3

PSO

4

TBBT-603 (III) CO 1. 2 - 2 1 2 - - 2 1 1 -

TBBT-603 (III) CO 2. 3 2 2 2 2 - - 2 2 2 -

TBBT-603 (III) CO 3. 2 - 1 2 2 - - 2 1 2 -

TBBT-603 (III) CO 4. 2 - 3 2 2 - 2 2 2 2 2

Average CO (TBBT-603

(III))

2.2 2.0 2 1.7 2 - 2 2 1.5 1.75 2

Page 172: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc (H) Biotechnology Programme Code 05

Course Code PBBT-603 (III) Credit 1

Year/Sem 3/6 L-T-P 0-0-2

Course Name Plant Diversity (I)Lab

Objectives of the Course:

1.To provide students basic understanding of various physiological processes in plants.

2.To provide basic knowledge of various organs in plants through representation slides relatedto

monocot, dicot, root, stem and male and female gametophyte.

3.To develop a deeper understanding of various photosynthetic pigments present in plant leafand

its quantitative estimation.

4.To learn about the growth and development in plants and to have basic knowledge of

planthormones, their role and function in relation to plants.

PRACTICALS

1. Comparative study of thallus and reproductive organs of various algae.

2. Comparative study of vegetative and reproductive parts of various fungi.

3. Study and section cutting and lectophenol mount of plant disease materials.

4. Study of various types of lichens.

5. Study of external features & anatomy of vegetative and reproductive parts of Marchantia and

Funaria

6. Collection of algae, fungi, plant diseases materials and bryophytes available locally.

Course Outcomes (COs)

PBBT-603 (III)CO1.Acquire skills about techniques related to plant physiology to prepare for

higher studies and employment.

PBBT-603 (III)CO2.Depict ability to identify and estimate various photosynthetic pigments found

in leaf and its application in botanical studies.

PBBT-603 (III)CO3.Identify ad appraise concept and mechanism of photosynthesis in plants.

PBBT-603 (III) CO4.Comprehend different process of scarification, role of different plant root

and steam growth and development of the plants and application of same in agriculture

References:

Books

1. Bajracharya, D., Experiments in Plant Physiology: A Laboratory Manual, Narosa Publishing

House, 1999.

Mode of Evaluation

Internal (MM-25) External (MM-25)

Component Internal End Semester Examination

Weightage 25 25

Page 173: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

CO-PO Matrix Plant Diversity-I Lab PBBT-603 (III)

Course Outcome PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7 PSO1 PSO2 PSO3 PSO4

PBBT-603 (III) CO 1. 3 - 2 2 2 - - 2 2 2 2

PBBT-603 (III) CO 2. 2 - 3 1 1 - - 2 2 1 -

PBBT-603 (III) CO 3. 2 - 2 1 1 - - 2 1 1 -

PBBT-603 (III) CO 4. 3 - 2 1 2 - - 2 2 2 -

Average CO (PBBT-

603 (III) 2.5 - 2.2 1.2 1.5 - - 2 1.75 1.5 2

Page 174: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc. (H) Biotechnology Programme Code 05

Course Code TBBT-604 (I) Credit 4

Year/Sem 3/6 L-T-P 4-0-0

Course Name Industrial Fermentation

Objectives of the Course:

1. To impart basic knowledge of experimental techniques associated with aseptic process,

media preparation and related upstream and downstream process.

2. To provide the knowledge related to needs of various parts of fermenter and their operation

in laboratory as well as industrial level.

3. To understand conditions that influence growth and fermentation in industry and research.

4. To gain the technical skills for scale-up in fermentation process from laboratory scale to

industrial scale.

UNIT I (Total Topics- 7 and Hrs- 10)

Introduction to Fermentation: Batch and Continuous, Types of Bioreactors, Industrial Production

of Propionic acid, butyric acid, acetic acid, citric acid, Biofuels: Biogas, Ethanol, butanol,

biodiesel; Microbial polysaccharides; Microbial insecticides, antibiotics, anti cancer agents,

amino acids.

UNIT II (Total Topics -12 and Hrs-10)

Microbial products of pharmacological interest, steriod fermentations and transformations.

Secondary metabolites – its significance and products. Metabolic engineering of secondary

metabolism for highest productivity.Enzyme and cell immobilization techniques in industrial

processing.

UNIT- III (Total Topics -8 and Hrs-10)

Downstream processing: Destruction of microbial cells, centrifugation, filtration of fermentation

broth, ultra centrifugation, liquid extraction, ion-exchange chromatography and drying for

recovery of biological products.

UNIT-IV (Total Topics -8 and Hrs-10)

Rate equations for enzyme kinetics, simple and complex reactions. Mathematical derivation of

growth kinetics and batch and continuous operations; mass transfer in aerobic fermentation;

overall mass transfer co-efficient (Ka) determination, Scale up.

Course Outcomes (COs)

TBBT-604 (I) CO1. Impart Knowledge regarding of microbial fermentation process for

production of value added products.

TBBT-604 (I) CO2. Understand the needs of various parts of fermenter and their operation in

laboratory as well as industrial level.

TBBT-604 (I) CO3. Designing and development of knowledge regarding fermentative media

for microbial cell cultivation for conducting various applications of fermentation in industry and

research.

TBBT-604 (I) CO4. Development of knowledge of scale-up in fermentation process from

laboratory scale to industrial scale. References:

Books

Page 175: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

1. Casida L.E. Industrial Microbiology. 1st edition. Wiley Eastern Limited.1991

2. Crueger W. and Crueger A. Biotechnology: A textbook of Industrial Microbiology. Panima

Publishing Co. New Delhi2002 2nd edition

3. Patel A.H. Industrial Microbiology Macmillan India Limited.1996 1st edition

4. Stanbury P.F, Whitaker A. and Hall S.J. Principles of Fermentation Technology, Elsevier

Science Ltd. 2006 2nd edition.

5. Salisbury, Whitaker and Hall. Principles of fermentation Technology

Mode of Evaluation

Internal (MM-40) External (MM-60)

Component Sessional -1 Sessional -2 Assignments End Semester Examination

Weightage 10 10 20 60

CO-PO Matrix Industrial FermentationTBBT-604 (I)

Course Outcome PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PSO

1

PSO

2

PSO

3

PSO

4

TBBT-604 (I) CO 1. 3 1 2 1 1 - - 3 3 2 1

TBBT-604 (I) CO 2. 3 2 2 - 1 - - 3 3 - 1

TBBT-604(I) CO 3. 3 - 1 - 1 - - 2 2 2 -

TBBT-604 (I)CO 4. 3 2 1 1 2 - - 3 2 2 1

Average CO(TBBT-604

(I)) 3 1.6 1.5 1 1.2 - - 2.7 2.5 2 1

Page 176: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc. (H) Biotechnology Programme Code 05

Course Code PBBT-604 (I) Credit 1

Year/Sem 3/6 L-T-P 0-0-2

Course Name Industrial Fermentation Lab

Objectives of the Course:

1. To impart basic knowledge of experimental techniques associated with aseptic process,

media preparation and related upstream and downstream process.

2. To provide the knowledge related to needs of various parts of fermenter and their operation

in laboratory as well as industrial level.

3. To understand conditions that influence growth and fermentation in industry and research.

4. To gain the knowledge of scale-up in fermentation process from laboratory scale to industrial

scale.

PRACTICALS

1. Comparative analysis of design of a batch and continuous fermenter.

2. Calculation of Mathematical derivation of growth kinetics.

3. Solvent extraction & analysis of a metabolite from a bacterial culture.

4.Perform an enzyme assay demonstrating its hydrolytic activity

(protease/peptidase/glucosidase etc.)

Course Outcomes (COs)

PBBT- 604 (I) CO 1.Demonstration of experimental techniques associated with aseptic process,

media preparation and related upstream and downstream process.

PBBT- 604 (I) CO 2.Acquire skills for need of various parts of fermenter and their operation in

laboratory level.

PBBT- 604 (I) CO 3.Designing and development of media for microbial cell cultivation for

conducting various applications of fermentation in industry and research.

PBBT- 604 (I) CO 4.Development of knowledge related to applications of fermentation in

bakery industry. References:

Books

1. Casida, L.E., Industrial Microbiology,Wiley Eastern Limited,1991, 1st edition.

2. Crueger, W. and Crueger, A., Biotechnology: A textbook of Industrial Microbiology, Panima

Publishing Co. New Delhi, 2000, 2nd edition.

Mode of Evaluation

Internal (MM-25) External (MM-25)

Component Internal End Semester Examination

Weightage 25 25

Page 177: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

CO-PO Matrix Industrial Fermentation lab PBBT-604 (I)

Course Outcome PO1 PO2 PO3 PO4 PO5 PO6 PO7 PSO1 PSO2 PSO3 PSO4

PBBT- 604 (I) CO 1 1 1 3 1 2 - - 2 1 2 1

PBBT- 604 (I) CO 2 - 3 - 1 - - 2 - 1

PBBT- 604 (I) CO 3 - 3 - - - 2 2 2

PBBT- 604 (I) CO 4 1 2 3 1 - - 1 1 2 1

Average CO PBBT-604 (I) 1 1.5 3 1 1.5 - - 2.3 1.3 2 1

Page 178: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc. (H) Biotechnology Programme Code 05

Course Code TBBT-604 (II) Credit 4

Year/Sem 3/6 L-T-P 4-0-0

Course Name Animal Biotechnology

Objectives of the Course:

1. To develop and design the fundamental aspects of animal biotechnology with detailed

applications in academic and industries.

2. To impart training on animal models using different biotechnological tools and techniques.

3. To impart potential application of animal biotechnology and genetic engineering at

commercial level to enhance development of diagnostic techniques, genomics, forensics and

health.

4. To promote research and Innovation in animal biotechnological research for benefit for

human health and improvement.

UNIT I (Total Topics- 9 and Hrs- 9)

Introduction to Animal Biotechnology, Laboratory organization of animal cell culture facility,

Animal cell lines, Cell culture media: natural and synthetic, serum based and serum free.

Continuous and discontinuous cell lines..

UNIT II (Total Topics -09 and Hrs-09)

Culture methods: Primary and secondary cultures. Gene transfer methods in Animals –

Microinjection, Embryonic Stem cell, gene transfer vectors. Introduction to transgenics – Mice,

Cow, Pig, Sheep, fish, Goat.

UNIT- III (Total Topics -08 and Hrs-08)

Animal propagation – Artificial insemination, Invitro fertilization, Animal Clones. Embryo

transfer techniques. Introduction to Stem Cell Technology and its applications

UNIT-IV (Total Topics -6 and Hrs-10)

Genetic modification in Medicine - gene therapy, types of gene therapy, vectors in gene therapy,

molecular engineering, human genetic engineering, problems & ethics.

Course Outcomes (COs)

TBBT-604 (II) CO 1.Understand the knowledge related to basic concept of animal

biotechnology for academics and higher research.

TBBT-604 (II) CO 2. Identify different types of animal cell culture, methods of gene transfer.

TBBT-604 (II) CO 3. Comprehend aspects related to basic principles and techniques in genetic

manipulation and genetic engineering in respects of animal model system.

TBBT-604 (II) CO 4. Acquire knowledge about animal genome applications, gene therapy,

human genetic engineering, stem cell technology, associated ethical issues and application for

human welfare. References:

Books

1. Thakur I. S., Environmental Biotechnology, I K Publication.

Page 179: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Mode of Evaluation

Internal (MM-40) External (MM-60)

Component Sessional -1 Sessional -2 Assignments End Semester Examination

Weightage 10 10 20 60

CO-PO Matrix Animal Biotechnology TBBT-604 (II)

Course Outcome PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PSO

1

PSO

2

PSO

3

PSO

4

TBBT-604 (II) CO 1. 3 2 2 3 2 - 3 2 1 2 2

TBBT-604 (II) CO 2. 3 - 3 - 3 3 - 1 3 - -

TBBT-604 (II) CO 3. 2 2 2 - 2 2 2 2 2 1 2

TBBT-604 (II) CO 4. 2 - - - - - - - - - -

Average CO(TBBT-604

(II)) 2.5 2.0 2.3 3.0 2.3 2.5 2.5 1.6 2.0 1.5 2.0

Page 180: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc. (H) Biotechnology Programme Code 05

Course Code PBBT-604 (II) Credit 1

Year/Sem 3/6 L-T-P 0-0-2

Course Name Animal Biotechnology Lab

Objectives of the Course:

1. To develop and demonstrate the experimental concept of animal biotechnology for

academics and higher research.

2. To design all aspects related to basic principles and techniques in genetic manipulation and

genetic engineering in respects of animal model system.

3. Identify the laboratory ethics related to work on gene transfer technologies for animals and

animal cell lines.

4. To develop laboratory experiments to enhancement of genetic disease and related to

diagnostic techniques.

PRACTICALS

1. Sterilization techniques: Theory and Practical: Glass ware sterilization, Media sterilization,

Laboratory sterilization

2. Analysis of sources of contamination and decontamination measures.

3. Preparation of Hanks Balanced salt solution

4. Preparation of Minimal Essential Growth medium

5. Study of lymphocytes for culturing

6. DNA isolation from animal tissue

7. Quantification of isolated DNA.

8. Resolving DNA on Agarose Gel Electrophoresis.

Course Outcomes (COs)

PBBT-604 (II) CO 1.Identify design and organization of animal biotechnology laboratory,

safety guidelines and applications.

PBBT-604 (II) CO 2. Appraise sterilization techniques, media preparation and laboratory

culture process.

PBBT-604 (II) CO 3. Demonstrate technical skill for isolation and quantitative estimation of

DNA and identify its application in biological research. .

PBBT-604 (II) CO 4. Comprehend principle, process and application of bioanalytical

techniques utilized in animal biotechnology. References:

Books

1.R. Ian Freshney. Culture of Animal Cells: A Manual of Basic Technique and Specialized

Applications, Sixth Edition. John Wiley & Sons publications

2.Srivastava, A. K. Singh, R. K. and Yadav M.P. Animal Biotechnology 2019

Mode of Evaluation

Internal (MM-25) External (MM-25)

Component Internal Assessment End Semester Examination

Weightage 25 25

Page 181: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

CO-PO Matrix Animal Biotechnology Lab PBBT-604 (II)

Course Outcome PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PSO

1

PSO

2

PSO

3

PSO

4

PBBT-604 (II) CO 1. 2 2 1 - - - 2 2 2 2 2

PBBT-604 (II) CO 2. 2 - 1 - - - - 1 2 - -

PBBT-604 (II) CO 3. 2 - - - 2 1 - 2 2 1 2

PBBT-604 (II) CO 4. 2 1 1 3 - - - - - - -

Average CO(PBBT-604

(II)) 2.0 1.5 1.0 3.0 2.0 1.0 2.0 1.6 2.0 1.5 2.0

Page 182: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc (H) Biotechnology Programme Code 05

Course Code TBBT-604 (III) Credit 4

Year/Sem 3/6 L-T-P 4-0-0

Course Name Animal Diversity - II

Objectives of the Course:

1. To learn the applied knowledge of animal diversity,classification along with their unique

fundamental morphologicalfeature in animal kingdom.

2. To make students learn the application of phylogeny in the field of modern biological science.

3. To familiarize the students through scientific enquiry into the nature of mechanical, physical, and

biochemical functions of animal system.

4. To provides theoretical and practical designing of recent kingdom classification techniques and

their conducting experiments in laboratories.

UNIT I (Total Topics- 10 and Hrs-10)

Proto-chordates: Outline of classification, General features and important characters of Herdmania,

Branchiostoma, Origin of Chordates, Pisces: Migration in Pisces, Outline of classification. Amphibia:

Classification, Origin, Parental care, Paedogenesis.

UNIT II (Total Topics -10 and Hrs-10)

Reptelia: Classification, Origin, Aves: Classification, Origin, flight- adaptations, migration

Mammalia: Classification, Origin, dentition.

UNIT- III (Total Topics -10 and Hrs-10)

Comparative anatomy of various systems of vertebrates: Integumentary, digestive, respiratory

systems.

UNIT-IV (Total Topics -10 and Hrs-10)

Comparative Anatomy of vertebrates – Heart, Aortic arches, Kidney &urinogenital system, Brain,

Eye, Ear. Autonomic Nervous system in Mammals.

Course Outcomes (COs)

TBBT-604 (III)CO1.Applied knowledge of biomolecules, metabolic pathways along with their

fundamental principles in the mammalian body at the cellular level and system level.

TBBT-604 (III)CO2.Understand the designing of recent physiological techniques with related to

human health system.

TBBT-604 (III)CO3.Knowledge and appreciation of mammalian physiology and understand the

functions of important physiological systems including the cardio, respiratory, renal, digestive and

endocrine systems.

TBBT-604 (III)CO4. Practicing group learning through scientific inquiry into the nature of

mechanical, physical, and biochemical functions of different systems of animal.

References:

Books

1.Kardong, K.V. Vertebrates Comparative Anatomy, Function and evolution McGraw-Hill Higher

Education.2005 4th edition

2.Kent, G.C. and Carr R.K. Comparative Anatomy of the Vertebrates. The McGraw-HillCompanies.

2000 9th edition.

3.Weichert, C.K. Anatomy of Chordate. McGraw Hill. 1970

Page 183: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

4.Young, J.Z. The life of vertebrates Oxford University press2004 3nd edition.

Mode of Evaluation

Internal (MM-40) External (MM-60)

Component Sessional -1 Sessional -2 Assignments End Semester Examination

Weightage 10 10 20 60

CO-PO Matrix Animal Diversity - II TBBT-604 (III)

Course Outcome PO1 PO2 PO3 PO4 PO5 PO6 PO7 PSO1 PSO2 PSO3 PSO4

TBBT-604 (III) CO 1. 2 2 - 1 - 1 3 2 - 2 2

TBBT-604 (III)CO 2. - - 2 - 2 1 - - 2 - -

TBBT-604 (III)CO 3. 2 2 - - - - 2 2 - 2 -

TBBT-604 (III)CO 4. - 2 2 - 2 1 - - 2 - 2

Average CO

(TBBT-604(III)) 2.00 2.00 2.00 1.00 2.00 1.00 2.50 2.00 2.00 2.00 2.00

.

Page 184: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc (H) Biotechnology Programme Code 05

Course Code PBBT-604 (III) Credit 1

Year/Sem 3/6 L-T-P 0-0-2

Course Name Animal Diversity – II Lab

Objectives of the Course:

1. To enable the students to acquire physiology based experiments of modern biomedical science.

2. To familiarize the students through scientific enquiry into the nature of mechanical, physical, and

biochemical functions of plant system.

3. To impart practical knowledge of fundamental principles in plant physiology.

4. To develop the theoretical based practical designing of recent physiological techniques and their

conducting experiments in laboratories.

PRACTICALS

1. Identification & Classification upto order of the following: Proto-chordata: Salpa, Doliolum,

Herdmania, BranchiostomaCyclostomata: Myxine, Petromyzon

2. Chondrichthyes: Scoliodon, Zygnea, Pristis, Trygon, Raja, Chimaera

3. Ostiechthyes: Labeo, Mystus, Catla, Hippocampus, Anabas, Echeneis, Lophius,

PolypeterusAmphibia: Rana, Hyla, Amblystoma, Necturus, Proteus.

4. Reptiles: Hemidactylus, Calotes, Draco, Phrynosoma, NajaVipera, BungarusAves: Columba,

Alcedo, Passer

5. Mammalia: Ornithorhynchus, Macropus, Didelphes, Dasypus

6. An Ecological Note on any one of the specimens in Experiment 1

7. Identification of the following slides. Mammalian Histology: Liver, Lung, Intestine, Kidney,

Ovary, Testes. Slides of Salpa, Doliolum, Spicules of Herdmania, Tadpole of Frog

8. Study of a permanent mount of Salpa, Placoid scales, spicules of Herdmania, Pharynax of

Amphioxus, Tadpole Larva of frog

9. Identification of endoskeletons

Course Outcomes (Cos)

PBBT-604 (III) CO1.Demonstrate the experimental techniques related to plant diversity at the

cellular level.

PBBT-604 (III)CO2. Understand the designing of recent physiological and morphological techniques

and their conducting experiments in laboratories.

PBBT-604 (III) CO3.Generate and interpret the test hypotheses, analyze the data by using modern

methods.

PBBT-604 (III)CO4.Practicing group learning through scientific inquiry into the nature of

mechanical, physical and biochemical functions of different flowering, reproductive system of plant.

References:

Books 1. Kent, G.C. and Carr R.K. Comparative Anatomy of the Vertebrates.The McGraw-HillCompanies.

2000 9th edition

Page 185: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Mode of Evaluation

Internal (MM-25) External (MM-25)

Component Internal End Semester Examination

Weightage 25 25

CO-PO Matrix Animal Diversity - II LabPBBT-604 (III)

Course Outcome PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PSO

1

PSO

2

PSO

3

PSO

4

PBBT-604 (III) CO 1. 2 2 - 1 3 1 3 2 3 2 2

PBBT-604 (III)CO 2. 1 - 2 - 2 1 - 2 2 - -

PBBT-604 (III) CO 3. 2 2 - - - - 2 2 - 1 -

PBBT-604 (III) CO 4. - - 2 - 2 2 - - - - 2

Average CO (PBBT-

604-(III) 1.6 2.0 2.0 1.0 2.3 1.3 2.5 2.0 2.5 1.5 2.0

Page 186: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc. (H) Biotechnology Programme Code 05

Course Code TBBT-604 (IV) Credit 4

Year/Sem 3/6 L-T-P 4-0-0

Course Name Plant Diversity-II

Objectives of the Course:

1. To provide a vast knowledge regarding plant growth technologies &modifications for a better

future in agriculture with help of analytical tools and technologies.

2. To acknowledge the various methods of cell culture, its importance & application, study of

usage of the certain micro flora aiding in the plants growth with help of techniques, data

interpretation, and implementing the acquired knowledge in biotechnological, medical and

agricultural fields with a lifelong understanding.

3. To widen the knowledge acquired by handling of classical and modern plant

biotechnology processes, including breeding of healthy plants with improved characteristics

and plants for biomolecules production to solve productivity problems.

4. Toimpart the understanding of the basic principles of the plant sciences on the molecular

level as well as the integration to encourage students to choose a desired career in plant

taxonomy.

UNIT I (Total Topics- 10 and Hrs- 10)

Pteridophytes: General characters of pteridophytes, affinities with bryophytes & gymnosperms,

classification, economic importance, study of life histories of fossil Pteridophytes – Rhynia

UNIT II (Total Topics -10 and Hrs-10)

Pteridophytes: Life histories of Selaginella- (Heterospory and seed habit), Equisetum, Pteris,

Lycopodium

UNIT- III (Total Topics -10 and Hrs-10)

Gymnosperms:General characters, classification, geological time scale, theories of fossil

formation, types of fossils, fossil gymnosperms- Williamsonia&Glossopteris, telome and steel

concept

UNIT-IV (Total Topics -8 and Hrs-10)

Gymnosperms:Life histories of Cycas&Pinus, economic importance of gymnosperms

Course Outcomes (COs)

TBBT-604 (IV) CO1.Understand the concept and principle of micropropagation technique and

identify designing of culture medium types of culture and application in agriculture and

biological research with emphasis on conservation studies.

TBBT-604 (IV) CO2.Identify the potential of micropropagation to produce haploids and

significance of involved techniques such as chromosome elimination with utility of such plants

in agriculture.

TBBT-604 (IV) CO 3. Demonstrate understanding of principle involved and technique adopted

for protoplast isolation and fusion and applicability to produce hybrid plants.

TBBT-604 (IV) CO 4.Exhibit knowledge about plant growth promoting bacteria free living

bacteria their impact on plants and environment and interpret applicability of hydroponics in

tissue culture studies. References:

Books

Page 187: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

1. Bhatnager, S.P. and Moitra, A. Gymnosperms. New Age International (P) Ltd. Publishers,

New Delhi. 1996

2. Parihar, N.S. The Biology and Morphology of Pteridophytes. Central BookDepot, Allahabad.

1996

3. Sambamurty A. Textbook of Bryophytes, Pteridophytes, Gymnosperms and Paleobotany. IK

International Publishers. 2008

CO-PO Matrix PlantDiversity-II TBBT-604 (IV)

Course Outcome PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PSO

1

PSO

2

PSO

3

PSO

4

TBBT-604 (IV) CO 1. 2 - - - - - - 2 - 3 1

TBBT-604 (IV) CO 2. - 2 - 1 - - 1 1 - 2 1

TBBT-604 (IV) CO 3. - - - - - - - 2 - 2 -

TBBT-604 (IV) CO 4. - 1 3 - - - - 1 - - -

Average CO(TBBT-604

(IV) 2 1 3 1.8 - - 1 1.5 - 2.3 -

Mode of Evaluation

Internal (MM-40) External (MM-60)

Component Sessional -1 Sessional -2 Assignments End Semester Examination

Weightage 10 10 20 60

Page 188: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc. (H) Biotechnology Programme Code 05

Course Code PBBT-604 (IV) Credit 1

Year/Sem 3/6 L-T-P 0-0-2

Course Name Plant Diversity-II Lab

Objectives of the Course:

1. To encompass tools&methods in cell culture which are used in plant identification.

2. To explain the concepts, principles and usage of the acquired knowledge of plant diversity

techniques in laboratory.

3. To acknowledge the use of herbarium file for invitro plantscollection.

4. To Handle analytical tools,learn safety measures, plant specimen handling and inculcating

the requirements for setup of a plant diversity laboratory PRACTICALS

1.Examination of morphology and anatomy of vegetative and reproductive parts of Selaginella,

Equisetum &Pteris.

2.Examination of morphology and anatomy of vegetative & reproductive parts of –

Cycas&Pinus

3.Plant collection (pteridophytes& gymnosperms)

Course Outcomes (COs)

PBBT-604 (IV) CO 1.Conduct cell culture experiments for conservation of plant species and

recognize significance of micropropagation in crop improvement

PBBT-604 (IV) CO 2.Acknowledgement of a ideal plant culture laboratory setup, its

components as well learning the handling of plant specimens and studying their core

characteristics

PBBT-604 (IV) CO3. Identify concepts and principles of various tools, techniques, utilized in

plant diversity laboratory alongwith data analysis and interpretation.

PBBT-604 (IV) CO 4.Comprehend various culture techniques, their application, recording

observations and analysis References: Books

1.Bhatnager, S.P. and Moitra, A. Gymnosperms. New Age International (P) Ltd. Publishers,

New Delhi. 1996

2.Parihar, N.S. The Biology and Morphology of Pteridophytes. Central Book Depot, Allahabad.

1996

3.SambamurtyA Textbook of Bryophytes, Pteridophytes, Gymnosperms and Paleobotany. IK

International Publishers. 2008

Mode of Evaluation

Internal (MM-25) External (MM-25)

Component Internal End Semester Examination

Weightage 25 25

Page 189: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

CO-PO Matrix Plant Diversity-II Lab PBBT-604 (IV)

Course Outcome PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PSO

1

PSO

2

PSO

3

PSO

4

PBBT-604 (IV) CO 1. 2 - - 1 1 - - - 2 3 -

PBBT-604 (IV) CO 2. - - 2 - 1 - 1 1 - 2 -

PBBT-603 (IV) CO 3. - - 3 1 - - 1 - 2 - 1

PBBT-603 (IV) CO 4. - 1 2 - 2 - - - 2 - -

Average CO(PBBT-604

(IV) 2 1 2.3 1.4 2 - 1 1 2 1.7 1.7

Page 190: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

Programme Name B.Sc. (H) Biotechnology Programme Code 05

Course Code ADP-605 Credit 4

Year/Sem 3/6 L-T-P 0-0-8

Course Name Dissertation(Major) & Presentation Lab

Objectives of the Course:

1. To acquire skills to operate various analytical techniques and instruments , identify their

applicability in research and utilize modern tools, e-resources for literature survey and data

compilation

2. To demonstrate technical skills to conduct laboratory based experiments and ability to record

observation and interpret data to derive a solution/conclusion to complex problem.

3. To exhibit competent writing (with critical analysis), communication and presentation skills

Guidelines:

• Students will have to do a project related to any assigned topic college or in industry/Research

Organization.

• Each student will submit 3 copies of hard bound in the department.

• Final evaluation will be done on the basis of quality of work, performance and presentation

Course Outcomes (COs)

ADP-605 CO1. Acquire skills to operate various analytical techniques and instruments ,

identify their applicability in research and utilize modern tools, e-resources for literature survey

and data compilation

ADP-605 CO2. Demonstrate technical skills to conduct laboratory based experiments and ability

to record observation and interpret data to derive a solution/conclusion to complex problem.

ADP-605 CO3. Exhibit competent writing (with critical analysis), communication and

presentation skills

Mode of Evaluation

Internal (MM-50) External (MM-150)

Component Internal Assessment End Semester Examination

Weightage 50 150

CO-PO MatrixDissertation(Major) & Presentation Lab ADP 605

Course Outcome PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PSO

1

PSO

2

PSO

3

PSO

4

ADP-605 CO1. 2 2 3 - - 1 2 2 2 3 2

ADP-605 CO2. 1 3 3 - - 2 2 2 3 3 2

ADP-605 CO3. - - - - 3 - - - - - -

Average CO(ADP-

605) 1.5 2.5 3

-

3

1.5

2 2 2.5 3 2

Page 191: Detailed Course Structure & Syllabus

UTTARANCHAL UNIVERSITY (Established vide Uttaranchal University Act, 2012)

(Uttarakhand Act No. 11 of 2013)

Arcadia Grant, P.O. Chandanwari, Premnagar, Dehradun, Uttarakhand

Uttaranchal University-Syllabus for Bachelor of Science- Hons. Biotechnology. w.e.f.-2020-23

VALUE ADDED COURSE

Course Code VAC-B6

Course Name RENEWABLE ENERGY TECHNOLOGY

Objectives of the Course:

1. To understand different forms of conventional resources of energy .

2. To analyse the environmental prospects, current scenario and needs of renewable energy

resources.

3. To understand the utilization of various sources of renewable energy for both domestics

and industrial application.

4. To attain knowledge about the principle and process involved in various renewable

resources of energy like Solar energy, Biomass Energy, wind Energy, etc. UNIT I (Total Topics- 6 and Hrs- 6)

Introduction: Introduction to renewable energy resources, Different types of Alternative Energy

resources. Fuel cells, Hydrogen gas, Geothermal.

UNIT II (Total Topics -12 and Hrs-8)

Bio-energy: Biomass energy sources. Biomass wastes, aerobic and anaerobic bioconversion

processes, Raw material and properties of bio-gas, Bio-gas plant technology and status, the

energetics and economics of biomass systems, Biomass gasification.

UNIT- III (Total Topics -8 and Hrs-8)

Biofuels: Biofuel Classification and generation, bioenergy crops:Woody biomass, Liquid

biofuels, bio-ethanol - issues & future prospects, Biodiesel – uses and production.

UNIT-IV (Total Topics -8 and Hrs-8)

Solar and Wind Energy: Fundamentals of Solar Photo Voltaic Conversion,Solar PV Power

Generation – Solar PV Applications. Wind energy conversion systems, Types of Wind Energy

Systems.

Course Outcomes (COs)

1. Understand the concept and operations of Biomass energy resources, Solar energy

resources and their utilization in domestics and industrial application.

2. Acquire knowledge about the need of renewable energy resources, historical and latest

developments.

3. Analyze the environmental aspects and their utilization of Biomass energy resources,

Solar energy resources in domestics and industrial application.

4. Understand the need of energy conversion and the various methods of energy conversion

and storage. References:

Books

1. Ashok Desai V, Non-Conventional Energy, Wiley Eastern Ltd, 1990.

2. Mittal K.M, Non-Conventional Energy Systems, Wheeler Publishing Co. Ltd, 1997.

3. Ramesh R, Kurnar K.U, Renewable Energy Technologies, Narosa Publishing House,

New Delhi, 1997.