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Developmental Biology
• The study of an organism from the time the egg is fertilized until birth
• During this time period the organism is called an embryo
• “The concept of an embryo is a staggering one, and forming an embryo is the hardest thing you will ever do. To become an embryo you had to build yourself from a single cell.”
Scott Gilbert
From single cell to adult - a complex task
• Developmental Biology has an extensive history, dating all the way back to Aristotle in 350B.C.!
• It is one of the fastest growing and most exciting fields in biology
• Developmental Bio is integrative - - – It incorporates molecular biology,
physiology, cell biology, genetics, anatomy, cancer research, neurobiology, immunology, evolutionary biology, and ecology
Developmental Biology: Historical Perspective
• Aristotle: 1st Embryologist – Observed incubated chicks egg daily for the three
weeks of development
• Two thousand years later (1600’s). . . William Harvey, Marcello Malpighi take a closer look (microscopes are available)– Malpighi supports the a theory called
preformation - all organs in are already present in the egg, but were miniature. This theory was believed true for many years.
1694 - Nicolas Hartsoeker depicts his theory that the entire human is already present in the head of the sperm
Developmental Biology: A Historical Perspective (cont.)
• The other school of thought felt that embryonic development occurred by “epigenesis.” This meant that all of the organs of the embryo were formed de novo (from scratch) at each generation
• Of course, modern biologists know that this is the case, but it was not until the 1700-1800’s that this view gained favor
• *Remember that the cell theory didn’t arise until the 1800’s*
Edward Alton, 1917
Embryology from classical to modern
• From the 1800’s until just a few decades ago, embryology was limited to observing the embryo as it developed and manipulating the embryo to see what would happen– Cutting out pieces, rearranging sections,
marking cells with dye to follow them along their path, culturing groups of cells away from the rest of the embryo
• Although these studies may seem limited today, early embryologists were able to elucidate a wealth of information
• These early studies resulted in a set of major questions that became essential to understanding development
The questions of Developmental Biology
• The question of Differentiation– How does an egg cell give rise to 100’s of
different cell types– Ex. Muscle cell, blood cell, neuron, fat
cells, gametes
Differentiation into many cell types
• The question of Morphogenesis– How do the cells in the embryo become
organized into specific structures, like organs?
– In other words, how do they take shape?– Organs also end up the the right places in
our bodies, relative to each other and the overall body plan - how?
• The question of Growth– During embryonic development, mitosis
occurs at astoundingly fast rates – How do our cells know when to stop
dividing?– How do cells on each side of our body
divide at similar rates so that we are symmetrical?
• The question of Reproduction– How do sperm and egg cells get set apart
and given the instructions to make the next generation of organism?
– What instructions are present in the nuclei and cytoplasm of the egg and sperm that give rise to the new organism?
• The question of Evolution– Evolution involves inherited changes in
developmental pathways– How do changes in development result in
the creation of new body forms? • Example: Organisms with wings vs. fins vs.
legs
• The question of Environmental integration– The environment of many organisms may
effect their development • Sometimes this is supposed to happen
– The sex of many reptiles is determined by the temperature at which their eggs incubate
• Sometimes exposure to environmental conditions is detrimental
– Exposure to chemicals during development can cause deformities
– In humans this is observed when fetuses are exposed to chemicals from smoking, alcohol, drugs, and other teratogens
The Questions of Developmental Biology
• Differentiation
• Morphogenesis
• Growth
• Reproduction
• Evolution
• Environmental Integration
“Classical” Embryologists designed specific experiments to address the
questions
• Observation• Fate mapping• Transplantation• Cell defect experiments• Isolation experiments• Recombination experiments
Isolation experiment
Defect Experiment
Transplant Experiment
• More recent advances in molecular biology, genetics and cell biology allow modern developmental biologists to revisit many of these “classical experiments”– Examine gene expression– Examine interactions of proteins– Examine how cells “talk” to each other– Explain why the results were obtained!
• We’ll examine these types of experiments during the semester
The Genetic Core of Development
• Before the discovery of DNA, and the modern understanding of genes, there was much debate about – heredity – how cells in the same organism could be different
from each other
• In fact it was not until the 1920’s that embryology and genetics emerged as separate– Genetics: the transmission of traits to next
generation– Embryology: the expression of those traits
The Genetic Core of Development
• The theory: genomic equivalence– Every cell in the embryo (and adult) has the same
nuclear information (passed on from the fertilized egg through mitosis)
• The problem (in the eyes of an embryologist)– If the somatic nuclei in each cell have the same
hereditary information, how can we end up with so many different cell types??
– WHAT DO YOU THINK?
The Proof
• We now know that the chromosomes in the nucleus contain all of our hereditary information, but how were embryologists convinced?
Cloning . . . Not as new as you might think!
• In 1952, Briggs and King replaced the nucleus of a fertilized egg with the nucleus of a cell from a frog blastula.
• The procedure was called Somatic Nuclear Transfer and is what we call “cloning”.
• The frog was identical to that of the donor embryo.
• INTERESTING POINT - transfers from later embryos - less successful
TOTIPOTENT
• Capable of directing the entire development of the organism!
The Genetic Core of Development
• Now that we know the nucleus contains all of the blueprints for development, we can design experiments that help identify genes and proteins involved in specific aspects of development.
Model Organisms - Why?• Can be studied easily in a laboratory• Have special properties that allow their
mechanisms of development to be easily observed – Clear embryos, large cells, etc
• Can be easily manipulated - physically or genetically
• Have relatively fast development period• Embryos can be readily obtained• Genomes have been mapped
C. Elegans - Nematode worm
Sea Urchin pluteus larva
Zebrafish embryo
Mouse embryo
Human embryo