7/24/2019 Differences in white and red muscle fibers distribution in trunk muscles of slow and fast-growing Russian sturgeon

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Differences in white and red muscle fibers distribution in

trunk muscles of slow and fast-growing Russian sturgeon

(Acipenser gueldenstaedti i) fingerlingsK. Marzecki, D. Adamek

Division of Ichthyobiology and Fisheries

Warsaw University of Life Science

ntroduction: Russian sturgeon (Acipenser gueldenstaedtii), according to the IUCN Red List, is a critically endangered fish from theAcipenseridaefamily. Oestocking is vital for the restoration of the natural state of the wild population. It is well known that growth rate may differ among and within sturgeon populations,

n slow and fast-growing phenotypes with different overall length and weight. Releasing only specimens with certain traits implicating they have higher than

robability of survival and successful reproduction, increases the effectiveness and decreases costs of restocking. The aim of this study was to determine the diffe

istribution of red and white muscle fibers in both groups.

Material and methods:Russian sturgeon fingerlings of the same cohort were reared in indoor recirculatory tanks in the Division of Ichthyobiology and Fisheries of Warsaw University of L

rom 6thdays post hatching (dph) until 23rddph, the fish were fed brine shrimpArtemia salina nauplii mixed with grinded starter Perla larva 6.0 (Skretting, Norway

3rddph sturgeons were fed commercial diets (Skretting, Norway) with the daily feeding rate set according to Kolman (2006). Specimens were devided into slow gr

ast growing (FG) and control (CG) groups, depending on body size. At the age of 100 and 200 dph, the trunk muscles for the histological analysis were collec

amples were fixed in Bouinssolution and stored at 4C. Samples were embadded in Paraplast, cut transversly into 5 msections and stained using hematoxylin-eo

Observations were made using NIS-Elements AR 2.10 software connected to Nikon Eclipse 90i microscope. Statistic analysis was performed using STATISTICA 10.

Results :

The distribution of muscle fibers was determined to be different in all

experimental groups. The highest percentage of small, hyperplastic (300m2) muscle fibers were more abun

observed in FG and CG groups (Fig. 1B;D).

Fig. 1. White (A-B) and red (C-D) muscle fiber distribution in three groups at 10

A

C

. 2.Muscle tissue histological slides stained hematoxylin-eosin; Scale bars= 10m.

G 100dph

CG 200dph

SG 200dph

G 100dph

G 100dph FG 200dph

Final remarks:

slow-growing sturgeons, though smaller, have far greater share of

hyperplastic muscle fibers

Slow-growing sturgeons are more likely to achive larger body len

weights.

Slow-growing sturgeons, having more white muscle fibers, are m

prolonged activity

More active fish search for food more eagerly, are harder to catch

and probably have better adaption to the natural environment.

More studies are needed on this phenomenon to improve current r

practices, reduce costs of rearing fish needed for restockings and

survivability of released specimens.

Acknowlegdgments: This work was financially supported by The National Centre for Researc

grant NR12-0129-10.

References: Kolman, R. (2006). Jesiotry Chwi hodowla [Sturgeons Breading and culture]. IRS, Olsztyn [in. 3. Size comparison between SG (upper) and FG (lower) sturgeon.