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Digital Microscopy : Improving Identification & Quantification in a Freshman Biology Classroom. Elizabeth Smith West Aurora High School RET fellow, 2010 University of Illinois-Chicago NSF Grant CBET-EEC-0743068. Outline. My RET experience My School, My Students Technology: - PowerPoint PPT Presentation
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DIGITAL MICROSCOPY:IMPROVING IDENTIFICATION & QUANTIFICATION IN A FRESHMAN BIOLOGY CLASSROOM
Elizabeth SmithWest Aurora High SchoolRET fellow, 2010University of Illinois-ChicagoNSF Grant CBET-EEC-0743068
OUTLINE My RET experience My School, My Students Technology:
Present & Future Objectives & Standards Lessons & Applications (6) Grant Request Acknowledgements References & Resources
BACKGROUND: MY RET AT UIC Dr. Michael Cho’s lab
Effects of Extracellular Topography on Human Mesenchymal Stem Cells
Our District (%)
State Averages
(%)
Low Income 45 45Mobility 18.6 13Limited-English Proficient 16.6 7.6Graduation Rate 71.3 87.8PSAE Meets & Exceeds: Reading Math Science Writing
41434141
54535255
BACKGROUND: MY SCHOOL, MY STUDENTS
BACKGROUND: MY SCHOOL, MY STUDENTS
3
18
62
Sections of Bi-ology
Honors BiologyBioTech ELL Bio
ALL students take some Biology
Biology: 50/50 Freshman &
Sophomores Most students:
Read below grade level ~30% of students have
parents that attend Open House or Conferences or attempt to contact the teacher
TECHNOLOGY
9 Biology classrooms Each has computer +
LCD projector 6 Compound Light
Microscope sets 13 scopes each
2 scope cams
Technology: Cameras are old,
only show B/W images
TVs are small, whole class can’t see
Students: Less than half have
used a microscope Students have little
concept of what they are seeing!
Current Technology Current Challenges
TECHNOLOGY
USB-based microscope ($700+)
OR USB-based microscope
camera ($350+)
1.3 Mega Pixel camera Calibration slide for
accurate measurementsSoftware: Motic Images
2.0 Capture stills & video Measurements (including
area) JPG, etc. to email/print Combine images Image processing & more!
A Solution MOTOCAM 1000
OBJECTIVES Use the MOTOCAM 1000 to:
Show the whole class proper microscope technique
Capture videos for later use & posting on school website Includes narration software
Capture images for statistical analysis Quantifying Biology! Compatible with Excel
ILLINOIS STANDARDSGoal 11
Inquiry and Design• A.3a Formulate
hypotheses that can be tested by collecting data.
• A.4b Conduct controlled experiments or simulations to test hypotheses.
• A.4c Collect, organize and analyze data accurately and precisely.
• A.4d Apply statistical methods to the data to reach and support conclusions
• A.3e Use data manipulation tools and quantitative and representational methods to analyze measurements.
• A.3g Report and display the process and results of a scientific investigation.
Goal 12Concepts and
Principles• A.3a Explain how cells
function as “building blocks” of organisms; describe the requirements for cells to live.
• A.5a Explain changes within cells and organisms in response to stimuli
• A.4b Describe the structures and organization of cells and tissues
• A.3c Compare and contrast how different forms and structures reflect different functions
Goal 13Science, Technology
and Society• A.4b Assess the
validity of scientific data by analyzing the results, sample set, sample size, similar previous experimentation, possible misrepresentation of data presented and potential sources of error.
• A.3c Explain what is similar and different about observational and experimental investigations
TEACHING: MICROSCOPY TECHNIQUES
Students learn how to use a microscope Wet mount slides
Letter “e” Crossed threads Printed paper
Misc. prepared Slides (bugs, etc)
Analyze Which way is the image
facing? What happens when you move
the slide left? Why can’t you focus on both
threads at once?
Show students what “in focus” looks like!
Avoid the “Bubble Eureka!” moment
Intro to Microscopes Digital Microscope Application
TEACHING: PLANT V. ANIMAL CELLS
Have students create & examine 3 cell samples: Onion skin cell Elodea cell Human Cheek cell
Analyze Compare & Contrast
the 2 plant cells Compare & Contrast
the plant v. animal cell
The first time that many students see cells! Most have no idea
what the are seeing: which things are cells?!
Capture stills Great for referring
back or for absent students
Capture video of moving chloroplasts
Comparing Cell Structures
Digital Microscope Application
TEACHING: OSMOSIS
Using Elodea samples, create a slide, observe
Flood the slide with saline solution, observe
Analyze What happens to
the cell? How could you
reverse the process?
Record video samples of the lab to share with absent
students & when teaching about
hyper-/hypo-tonic solutions
Watching Water Move Digital Microscope Application
TEACHING: MITOSIS & THE CELL CYCLE
Use Onion Root tip or other premade mitosis slides
Students find & sketch examples of interphase, mitosis (PMAT), and cytokinesis
Identify cell membrane, cell wall, chromosomes, cleavage furrow, cell plate
Analyze Compare & contrast mitosis
in plant & animal cells
Before lab, use digital projector to identify the parts of mitosis in real cells
In lab, have students take turns capturing & printing images of different parts of the root tip. Count the # in each phase Create a graph to show
number of cells in each phase
Analyze: Which phases are the longest? Shortest?
Seeing Mitosis Digital Microscope Application
TEACHING: POND WATER & PROTISTS
Depression slide + pond water,
Sketch, identify, and label the structures. Cell membrane, cytoplasm,
chloroplasts, cilia, flagella, nucleus
Analyze Beside each, describe:
Unicellular or multicellular? Autotroph or heterotroph? How does this protist move? How does this protist feed?
Have the class work as a group to capture an image of each new organism
Volvox & ameobae are rare most years; capture a video to share with other classes
Use pictures captured to label in later assessments
Protists in the Water! Digital Microscope Application
TEACHING: PLANT ADAPTATIONS
Have students exam cross sections of a dicot leaf; draw & label: Cuticle, epidermis, palliside
mesophyll, spongy mesophyll, vascular bundle (xylem + phloem) stomata, guard cells
Analyze Describe how each helps
with photosynthesis or protection
Have each group capture a picture of the epidermis from a tropical & a desert biome Count the number of
stoma in the same size area
What differences do you see? Why?
Anatomy of a Leaf Digital Microscope Application
TECHNOLOGY REQUESTED
Quote by Scope Shoppe (scopeshoppe.com)
$500 UIC/NSF FundingNSF Grant CBET-EEC-0743068
$218 West Aurora High School
(44% funding match)
$718Two (2) MOTICAM 1000 Digital Microscope cameras Software Included$359 each (Free Shipping)
Total students affected?
750Every Year
ACKNOWLEDGEMENTS NSF Grant CBET-EEC-0743068 Prof. A. Linninger, RET Program Director Dr. Michael Cho, Research Mentor Brandon Lutz and Hannah Wirtshafter, fellow
researchers University of Illinois- Chicago
REFERENCES & RESOURCES Illinois Interactive Report Card
http://iirc.niu.edu/ Swift Optical Resources
http://www.swiftoptical.com/EducationalResources.aspx Scope Shoppe
www.scopeshoppe.com Pictures
http://www.subbody.net/01subbody/Unfold/240/071013_volvox.jpg http://www.kuhnphoto.com/gallery/biology/microscopic/stomata.jpg http://faculty.irsc.edu/FACULTY/TFischer/bio%201%20files/test-yourself%20mitosis.j
pg https://
kleinsclasses.wikispaces.com/file/view/handbook-microscope_noshadow-T.jpg/56048204/handbook-microscope_noshadow-T.jpg
http://www.workshopplus.com/productcart/pc/catalog/moticam1000setup_809_detail.jpg
http://www.tedpella.com/cameras_html/camera2.htm http://www.ndt-educational.org/images/Artefatti26.jpg http://wikidoc.org/images/a/a6/Chloroplasten.jpg