Direct imaging reveals stable, micrometer-scale lipid domains that

segregate proteins in live cells

Alexandre Toulmay and William A. Prinz

Journal of Cell Biology July 8 2013, vol 202 p35-44

LIPID RAFTS.

1:1 mixture of phosphatidyl choline and sphinglomyelin

1:1:1 mixture of phosphatidyl choline, sphingomyelin, and cholesterol

Saccharomyces cerevisiae: vacuole.

Toulmay and Prinz (2013) Fig. 1a

Toulmay and Prinz (2013) Fig. 1b

Toulmay and Prinz (2013) Fig. Suppl. 1a

Toulmay and Prinz (2013) Fig. Suppl. 1b

Toulmay and Prinz (2013) Fig. Suppl. 1b

Ncr1-GFP Ivy1-GFP

Toulmay and Prinz (2013) Fig. 1c

Toulmay and Prinz (2013) Fig. Suppl. 2a

Toulmay and Prinz (2013) Fig. Suppl. 2b

Toulmay and Prinz (2013) Fig. Suppl. 2b

Toulmay and Prinz (2013) Fig. 2a

Toulmay and Prinz (2013) Fig. 2b

Toulmay and Prinz (2013) Fig. 2c

Methyl beta cyclodextrin (MbCD).

Toulmay and Prinz (2013) Fig. 2d

Toulmay and Prinz (2013) Fig. 2E

Toulmay and Prinz (2013) Fig. 3a

Toulmay and Prinz (2013) Fig. 3b and c

Location of Laurdan (and Prodan) in phospholipid bilayer.

Parasassi T et al. (1998). Journal of Fluorescence vol 8 (4) p365-373.

Emission spectra of Laurdan with different phase states (gel vs fluid), i.e., temperature of phospholipid bilayer.

Parasassi T et al. (1998). Journal of Fluorescence vol 8 (4) p365-373.

Increased amount of water (black dots) around Laurdan in liquid crystalline state causes the red shift in the emission peak of fluorescence.

Sanchez SA et al. (2007). Modern Research and Educational Topics in Microscopy.

Toulmay and Prinz (2013) Fig. 3d

Toulmay and Prinz (2013) Fig. 4a

Toulmay and Prinz (2013) Suppl. Fig. 3a

Vacuoles of log Vph1-GFP cells grown in FM4-4.

Toulmay and Prinz (2013) Suppl. Fig. 3b

Vacuoles of stat Vph1-GFP cells grown in FM4-4.

Toulmay and Prinz (2013) Fig. 4b, c, and d

Toulmay and Prinz (2013) Fig. 4e

Toulmay and Prinz (2013) Fig. 5a

Toulmay and Prinz (2013) Fig. 5b

Toulmay and Prinz (2013) Fig. 5c

Toulmay and Prinz (2013) Fig. 5d

Toulmay and Prinz (2013) Fig. 5e

Toulmay and Prinz (2013) Fig. 5e