Direct Sugar to HydroCarbon Route Synthesized Iso-Paraffins from Hydroprocessed Fermented Sugars April 23, 2014 – ABLC Conference Moving Forward with Sustainable

Direct Sugar to HydroCarbon RouteSynthesized Iso-Paraffins from Hydroprocessed Fermented Sugars

April 23, 2014 – ABLC ConferenceMoving Forward with Sustainable Alternative Jet Fuels Development and Qualification Panel Discussion

Fernando GarciaAmyris, Inc.Senior Director, Scientific and Regulatory Affairs [email protected]

mailto:[email protected]

Status of SIP Fuel Qualification DSHC route produces an Synthesized Iso-Paraffins (SIP) farnesane, for use in

conventional Jet A-1 fuel • An industrial process combining fermentation and hydroprocessing steps• A high purity iso-paraffin grade (farnesane) as a blending component for conventional jet fuel• The neat molecule meets compositional specifications of SPK alternative jet fuel

Blends of farnesane in conventional jet fuel up to 50% meet the requirements of Aviation Turbine Fuels Containing Synthesized Hydrocarbons from ASTM D7566

• Farnesane improves specific performance of the blends such as the freezing point, the thermal stability and the net heat of combustion

Blends of farnesane in conventional jet fuel up to 20% meet the Fit-For-Purpose requirements described in ASTM D4054

Engines and aircraft were operated on blends at 10 and 20%• Data from engine and flight tests show no deviation between blend and baseline Jet A-1• Data from Honeywell APU and P&W combustor tests support proposed specifications

Balloting ASTM approval for the use of farnesane at 10% blend in conventional jet fuel• Research Report has been successfully balloted• Presently balloting new Annex to ASTM D7566

2 Moving Forward with Sustainable Alternative Jet Fuels Development and Qualification Panel Discussion, April 23, 2014

Amyris - Total partnership since June 2010A specific partnership model to develop the value chain integrated from biomass to finished products

Bio-engineering of micro-organisms

R&D network

Synthetic biology

1st generation in Brazil on sugar cane

Leading to 2nd generation

Feedstock sourcing

Track record for strain improvement

Scale-up and industrial chemistry

Fermentation and chemical processes

Fuels Special fluids Lubricants Chemicals

Competitive molecules

Access to markets

Trading & marketing

BiomassFermentable raw materials

MoleculesCiHj

Products

Downstream ProcessingFermentationPre-treatment

Differentiating technology: broad R&D collaboration (~$100M) Lab-to-market development: joint industrialization & commercialization

Synergy: assemble complementary technology, industrial and business capabilities

Moving Forward with Sustainable Alternative Jet Fuels Development and Qualification Panel Discussion, April 23, 20143

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Manufacturing process based on common industrial steps

Microbe-catalyzed conversion of sugar: key is the development of a farnesene producing yeast

Production to date: >4 million liters of farnesene precursor Farnesene plant with a capacity of up to 50 million liters per year at

target efficiency in Brotas, Brazil

FarnesaneFarnesene

Hydroprocessing

SugarFermentation

Downstream processing

Purification

Fermentation


• Combination of hydroprocessing and separation operations

• Production to date: ~2 million liters of farnesane for diesel application

• Using diesel process slightly modified, farnesane aviation grade production to date for ASTM test purposes: ~ 30,000 liters

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Testing Collaboration with Industry

SNECMA SaM146 engine test

Performances, cold and hot starts and sub-idle & off-idle tests data did not present significant deviation between 10% blend and Jet A-1

Lufthansa CFM56 engine test

The study concluded that in emission terms the farnesane blends perform similar to or better than the reference kerosene. In particle mass there is a clear reduction at all test points by increasing farnesane content. In performance terms, the farnesane blends perform similar to the reference kerosene.

Airbus experimental flights (A321 with CFM56-5B)

The engine, supplied with the 10% blend, performed exactly like the x-wing sister engine fed with pure Jet A-1

APU (Honeywell ) and Combustor tests (P&W) funding by DOT/FAA CLEEN)

Analysis of fuel droplet size, ignition characteristics with combustor rigs, and APU cold and altitude starting in test chamber.


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Commitment to Quality

Air Total • High-quality aviation fuels, under the most stringent Safety and Environmental standards• Complete range of services dedicated to aviation (technical inspections, refueling cards, etc.)

SIP Fuel Assays• 19 assays with 30 characteristics

• Only nine assays are common with commercial Jet A-1

• Qualifying labs that run GC farnesane purity

Process Quality Control Plan (QCP)

An end-to-end (Farnesene-to-Jet Grade Fane Delivery) QCP has been developed, reviewed with the Total Quality team and is currently being implemented at Brazil operations based on ‘guard banding’ the Product specs by 3*Sigma of assay variation



Safety Assessment

ISO 17025 Section 4: Management Requirements

Management & document control systems, NCR processes (including cause analysis, CAPA implementation and monitoring), training processes and records, internal/external audits

ISO 17025 Section 5: Technical Requirements

SQC documentation, analyst expertise, instrumentation, maintenance programs, calibration processes, data records, test report formats and review process, data control, OOC processes

Test Method Performance

Capability per ASTM Requirements: - Repeatability: based on replicate analysis- Reproducibility: based on inter-lab round robin assessment & SQC charts

Commitment to Quality: 3rd Party Lab Validation

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AMYRIS: [email protected] TOTAL: [email protected] and Total contacts

Moving Forward with Sustainable Alternative Jet Fuels Development and Qualification Panel Discussion, April23, 2014

Documents

Direct Sugar to HydroCarbon Route Synthesized Iso-Paraffins from Hydroprocessed Fermented Sugars April 23, 2014 – ABLC Conference Moving Forward with Sustainable