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e-portfolio for BMY3101 (Microbiology I), Dr Wan Zuhainis
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B I O G R A P H Y
Nurdiyana Binti Abdullah • 28 July 1992 • 15’s college • Kuala Pilah, Negeri Sembilan • love Microbiology & Biochemistry, love photography & design •
from diploma in Medical Laboratory Technology •
In this course, BMY3101, I learn about the basic in microbiology. The first topic is the introduction to microbiolgy, studying microorganism through microscopic technique, prokaryotic and eukaryotic cells, non-cell microorganism, sterilization and disinfection, antimicrobial chemotherapy, microbial nutrition and metabolism, microbial genetic and lastly epidemiology and public health. This course is taught by Dr wan zuhainis bt Saad, and Prof dr. raja Noor Zaliha.
In this topic, I learn about the major
group of organism studied in microbi-ology such as bacteria, algae, fungi, protozoa and virus and their taxonomy. Besides that, I now know who the father of Microbiology is. His name is Antonie van Leeuwenhoek. Without his discovery, we might not know the small creatures that live together with us. Not just him, so many scientists had discovered and study the theory involving lives. I’m so interested to know about the golden age of microbiology, I feel so proud on how much the microbiology field has con-tribute to this world societies for example, the production of vaccines has save many lives since that time.
INTRODUCTION TO MICROBIOLOGY
This topic brought me into a new di-
mension of microscopic technique where
I learn that so many microscopes have
their own unique function. For example,
Scanning Electron Microscopy, we can
view the surface of the cell in 3D that is
in my opinion, it is so amazing. I wish
one day I will use this kind of microscope
in my research. Not only that, in this
topic, I also learned about the slide
preparation which is going to be observe
under the microscope. Especially for light
microscope, the staining is very crucial.
From the staining we can classified the
bacteria into two group, gram positive
and gram negative bacteria. This kind
classification for me is really important
because we can identify the bacteria eas-
ily if we know its classification.
STUDYING MICROORGANISM THROUGH MICROSCOPY TECHNIQUE
This topic defines me what prokaryotic cell
is. Firstly, I am able to describe the structure of
the cells, the shape and the arrangements. From
my opinion, this topic is quite connected with
previous topic, because, after the slide prepara-
tion, the staining and observation under the
microscope leads to the discovery of how the
cell looks like. Whether its shapes (cocci and
bacilli) or its arrangement (streptococci, in
chain, or staphylococci, in clusters). This topic
also focusing on the cell structure. It is impor-
tant to have the knowledge to differentiate be-
tween the structure of prokaryotic and
eukaryotic cell. The extracellular structure
includes the cell wall and cell membranes that is
from my point of view, it is very important to the
protection of cell itself and for the intracellular,
it is important to drives the function of the cells
and to carry on their lives.
ORGANIZATION & STRUCTURE OF PROKARYOTIC CELLS
After the study of prokaryotic cells, it is important to study
about eukaryotic cell also. Not only human and animal, but in the
study of microorganism, some of its group of organism falls into
this specification. They are fungi and protist. This kind of cells
carried out complex function and not only that, they having more
organelles inside their cells to carry out those complex functions.
In this topic, I have the bird-eye-view on protozoa, fungi and vi-
rus. They are called as non-cell microorganism because they fall
into the category of eukaryotic cells which means they carry vari-
ous functions and to be easy, they are protista. Protozoa is animal
-like protists and belongs to group of Mastigophorans, Amebozoa,
Apicomplexan and Ciliates. Fungi, it resembles the plant, but it is
heteretroph and many of them are saprophyte, differ with plant,
fungi digest dead organic matter and organic waste. Fungi plays
important role in ecosystem, as we know, it is a decomposers.
Then, in the context of fungi, I also learn about lichens and algae.
Lichen is an association of a fungus and photosynthetic symbiont
resulting in a stable thallus. From what I learn, Lichen acts as a
primary succession in the ecological role. They make abiotic
world habitable to biotic world. The algae also resemble plant;
they have chlorophyll that means they are photoautotropic. The
simplest example that we can see is seaweed. Not only providing
the habitat to marine communities, it is also a human food. Did
you ever heard about seaweed crackers and sushi?
EUKARYOTIC CELLS
When it’s come to the word
non-cell, I would expect the
non-living thing. What is the
amazing thing about the non-living
in the context of microorganism; it
is exactly a virus, viriod and prions.
They are non-living, but they have
either DNA or RNA which means
they are able to replicate. Prions on
the other hand, really shock me
because, they protein but don’t have
nucleic acid. To study the virus,
another technique of isolation must
be learning. It is because they are
obligate intracellular parasites,
which means they need live cell in
order to live.
NON-CELL MICROORGANISM
For a microbiologist, the ster-
ilization and disinfection are really
important to them. Whether it
means to kill the organism or to
grow them. When we are going to
grow a certain bacteria on the
media for example, it is important
to disinfect and to sterile the wire
loop or any equipment needed to
prevent the contamination. Same
goes to killing the microbes; we
sure don’t want any of them to fol-
low us to home and having fun
with our family.
STERILIZATION & DISINFECTION
For me, antibiotics are another
way of killing microbes especially the
pathogenic one. But recently, many of
the bacteria have developed resistance
to it. I think it is important for me, as a
future microbiologist to find a new
antibiotic. The most shocking news is
there is a microbe that already resis-
tance to the antibiotic of last resort
which is vancomycin. From now on, I
would advise any of my family
members and friends to take
antibiotics following the medical
prescription. Because for me, I think
bacteria develop this kind of resistance
is due to misuse of antibiotic itself.
ANTIBIOTICS
In order to identify a bacterium, it
is important for us to grow and
isolates the desired bacteria first. In
this topic, I learned how to culture
the bacteria, using a few culturing
technique such as plate culture or
broth culture. I also learned the
types of growth media that is needed
for a certain bacteria to grow. After
obtaining the single colony, then we
can do further testing for the
identification of the bacterial species.
CULTURING TECHNIQUES, ISOLATION OF BACTERIA AND TYPES OF GROWTH MEDIA
In this topic, I learn about the
metabolism of bacteria. I’m so inter-
ested when in the notes; Dr. said
there is there is some cycling of
elements in ecosystem that can only
be done by microbes such as organic
nitrogen cycle. The metabolism also
affected by enzymatic reaction factor.
Enzyme has really important
function in our body and carried out
crucial role in metabolism. I also
learn the metabolism pathway, ATP
generating mechanism and even
defining the metabolism meaning.
MICROBIAL METABOLISM
Nutrition to a microorganism
involves two goals that are source of
carbon and source of energy. In these
topics I learn about the nutritional
requirement for the microorganism.
Some of them are photoautotropic,
photoheterotroph, chemoautotroph
and chemoheterotroph. It is important
to know the definition of each of the
nutritional requirement because it
defines what exactly the microorgan-
ism need is.
MICROBIAL NUTRITION
This second last topic is taught by
Prof Dr. Raja. This topic is quite tough
for me because it require a lot of
understanding rather than memorizing.
The genetic topic is really important in
microbiology course because there are a
lot of thing that we going to research is
definitely involving the genetics.
Without genetic it is impossible to truly
identify a new species of microbe and
the resistance pattern of the microbes
toward antibiotic.
INTRODUCTION TO MICROBIAL GENETICS
Public health is the main
concern to any of the
microbiologist. In this topic I
learned about the spread of the
disease to human, whether it is
connected to pathogenic bacteria,
virus or fungus. It is so crucial to
study the epidemiology of
microbes in order to prevent its
spread, to control the effect of the
microbes to the community and to
give the awareness to the public in
preventing the spreading of germs.
MICROBIAL EPIDEMIOLOGY & PUBLIC
My thoughts….
“ after taking this course, I know the basic of
microbiology and I thinks Dr Wan is very
passionate people. I believe that she had
successfully made everyone love
microbiology so much, she also brings
learning process into a new level. I taught on
how to do assignment, quizzes, exploring
microbiology via internet”