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伯森生物科技股份㈲限公司Blossom Biotechnologies Inc.
伯森網站:www.blossombio.com / 客服專線:台北 0800-059668 台南 0800-259988 花東 0800-056668 / 電子信箱:[email protected]
台北 (02) 2788-2121 新竹 (037) 581-106 花東 0800-056668
台中 (04) 2323-3939 台南 (06) 235-6628 高雄 (07) 555-0764
HDAC & Sirtuin Enzyme Activity Assay Kits
Enzo�Life�Sciences�是全球第⼀個研發出⾮放射性� HDAC� (Histone�Deacetylase)� 酵素活性檢測試劑的公司,其所推出的各類組蛋⽩後轉譯修飾偵測試劑,皆採⽤⾼品質的專利受質及顯⾊劑� (sub-strate/developer),同時搭配⾼活性、純度的酵素,讓研究者可以⾼效準確地篩選出潛⼒藥物。
產品優勢
l 偵測⽅式多元:包括可⾒光、化學冷光、及螢光三種⽅式可供選擇。
l 實驗流程簡單:全程不需清洗,適合⾼通量需求者。
l 避免⼲擾現象:專利研發的�®Chemilum� de� Lys � 化學冷
光受質,可有效降低各類�Inhibitors ,� Lys is� deter-gents� 所造成的⼲擾現象,避免偽陽性與偽陰性結果。
HDAC/Sirtuin Assay Kits
Eliminate Interference Seen in Luciferase-based Assays
Eliminate False HDAC/SIRT Screening Hits with Chemilum de Lys®
Competitor HDAC Assays
Luciferase-basedChemiluminescent Assay
Chemilumde Lys®
ColorimetricAssay
FluorometricAssay
No Artifactual activationby resveratrol
Resistant to Protease Inhibitors
Resistant toKinase Inhibitors
Colorimetric
Chemiluminescent
Fluorometric - General HDAC/SIRTUIN Assays
Fluorometric - Enzyme Specic HDAC/SIRTUIN Assays
Color de Lys® HDAC colorimetric activity assay kit
Chemilum de Lys® HDAC/SIRT Chemiluminescent drug discovery kit
Fluor de Lys® HDAC Cellular Activity Assay Kit
Fluor de Lys® HDAC Activity Assay Kit
Fluor de Lys® Green HDAC Activity Assay Kit
Fluor de Lys® HDAC1 Drug Discovery Kit
Fluor de Lys® Green HDAC2 Drug Discovery Kit
Fluor de Lys® HDAC3/NCOR1 Drug Discovery Kit
Fluor de Lys® HDAC6 Drug Discovery Kit
Fuor de Lys® HDAC8 Drug Discovery Kit
Fluor de Lys® SIRT1 Drug Discovery Kit
Fluor de Lys® SIRT2 Drug Discovery Kit
Fluor de Lys® SIRT3 Drug Discovery Kit
Fluor de Lys® SIRT5 Drug Discovery Kit
Fluor de Lys® Green SIRT5 Drug Discovery Kit
Product Type Product Name
BML-AK501
BML-AK532
BML-AK503
BML-AK500
BML-AK530
BML-AK511
BML-AK512
BML-AK531
BML-AK516
BML-AK518
BML-AK555
BML-AK556
BML-AK557
BML-AK513
BML-AK514
Cat. No.
⽬前已知異常的組蛋⽩修飾�(Histone�Modification)�與多種癌症及疾病具有⾼度相關性,�因此可藉由找尋調控此機制的⽅式,�開發具有潛⼒的治療藥物。本刊主要針對組蛋⽩修飾相關分析⼯具進⾏介紹,�所有產品皆來⾃國際知名⼤廠��Enzo�Life�Sciences,�Eurofins�Scientific,�及�PerkinElmer。歡迎踴躍洽詢伯森業務專員索取更詳盡的表觀遺傳學研究產品資訊,�以及最新優惠訊息。
EPIGENETICS RESEARCH TOOLSEPIGENETICS RESEARCH TOOLSEPIGENETICS RESEARCH TOOLSEPIGENETICS RESEARCH TOOLS
The compound, PTC124, is converted
by luciferase in the presence of ATP to
a high afnity multi-substrate adduct
inhibitor, PTC124-AMP. PTC124 at
concentrations greater than 1 µM
inhibits the luciferase based assay, but
has no effect on the Chemilum de
Lys® assay.
PTC124
0 µM 0.01 µM 0.1 µM 1 µM 10 µM 100 µM
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
1.0
1.1
0.0
RL
U
Competitor P®
Chemilum de Lys
伯森網站:www.blossombio.com / 客服專線:台北 0800-059668 台南 0800-259988 花東 0800-056668 / 電子信箱:[email protected]
® ®AlphaLISA and LANCE Ultra Assays
Biochemical Epigenetic Assays
® ®由美商�PerkinElmer�獨家研發的�AlphaLISA �及�LANCE �Ultra�系列
產品,可依據您的需求彈性設計實驗,分析過程無需清洗或分離步
驟,能夠⼤幅簡化並縮短實驗時間;同時具有提⾼偵測靈敏度、節
省珍貴樣品⽤量的優點,特別適合有⾼通量藥物篩選需求的研究者
使⽤。
產品特點
®l AlphaLISA :⾼靈敏度、應⽤彈性,可應⽤於�Biochemical�與�Cell-based�assay。�(Peptide�與�Full-length�protein�型式的酵素受質皆可適⽤偵測。)
®l LANCE �Ultra:極⾼靈敏度,適⽤於�Biochemical�assay。�(僅適⽤於�Peptide�型式的酵素受質,不適⽤於全⾧蛋⽩。)
® ®利⽤�AlphaLISA �及�LANCE �Ultra�技術,搭配專⼀性的�Peptide�作為酵素受質�(enzyme�substrate),以偵測甲基化�
(methylation)、或⼄醯化�(acetylation)�等修飾酵素活性。可作為化合物或藥物篩選平台。
High-throughput Epigenetic Assays
Detection of reaction products occurs via mark-specic antibodies coupled to Acceptor beads (AlphaLISA®) or labeled with europium chelate
(LANCE® Ultra). The biotin substrates is captured by streptavidin coupled to a Donor bead (AlphaLISA®) or labeled with the ULight™ acceptor dye
(LANCE® Ultra). In both assay technologies, irradiation of the captured reaction products triggers an energy transfer leading to light emission
proportional to the enzyme activity.
AlphaLISA® LANCE® Ultra
伯森網站:www.blossombio.com / 客服專線:台北 0800-059668 台南 0800-259988 花東 0800-056668 / 電子信箱:[email protected]
Cell-based Epigenetic Assays
®針對細胞萃取物�(cell�lysates)�樣本的偵測實驗,具有多款便利的�AlphaLISA �偵測試劑套組可供選擇,可直接適⽤於
偵測�endogenous�histone�protein�修飾情形。
Schematic representation of the AlphaLISA®
cellular assay for the detection of modied histone
proteins. Histone H3 is extracted from cell lysates
and detected with a biotinylated Anti-H3 antibody
which binds streptavidin Donor beads and anti-mark
antibody-coated AlphaLISA® Acceptor beads
specic to the particular modication (acetylated or
methylated residue).
Order Information
H3K4 unmodied
H3K9/K27 unmodied
H3K4me1-2
H3K9ac
H3K9me2
H3K27ac
H3K27me2-1
H3K27me3
H3K36me2
H3K79me2
H3R2me
H3/H4Rme pan
H4Kac pan
H4R3me
H4K20me1
O-linked GlcNAc
P53 K382ac
LSD1, Sirtuin 1, HDAC2
HDAC1, JMJD1A
SET7/9
p300
G9a, JMJD2A, JMJD2C
HDAC1
EZH2, JMJD3
EZH2
JMJD2A
DOT1L
PRMT6
PRMT4
KAT5
PRMT1
SET8
OGA
Sirtuin 1
AL119
AL138
AL116
AL114
AL117
AL120
AL121
AL122
AL123
AL148
AL139
AL151
AL143
AL150
AL145
AL144
AL124
AL719
AL716
AL714
AL717
AL720
AL721
AL722
AL723
AL748
TRF0404
TRF0411
TRF0402
TRF0400
TRF0403
TRF0405
TRF0406
TRF0407
TRF0408
TRF0415
TRF0412
TRF0414
TRF0413
TRF0409
Mark Detected Validated EnzymeAlphaLISA
1(Biochemical)AlphaLISA
(Cell-based)LANCE Ultra
2(Biochemical)
(1). Additional Items Required for AlphaLISA histone modication biochemical assays: AlphaScreen Streptavidin Donor beads (Cat# 6760002),
AlphaLISA biotin-anti-histone H3 (C-ter) antibody (required for full-length Histone H3 substrates) (Cat# AL118), AlphaLISA biotin-anti-histone
H4 antibody (required for full-length Histone H4 substrates) (Cat# AL146), and AlphaLISA 5X Epigenetics Buffer 1 kit (Cat# AL008).
(2). Additional Items Required for LANCE Ultra histone modication assays: LANCE Ultra ULight-Streptavidin (Cat# TRF0102), and LANCE
Detection Buffer, 10X (Cat# CR97-100).
更多 Epigenetics 研究工具歡迎洽詢伯森業務專員 O 0800-059668
伯森網站:www.blossombio.com / 客服專線:台北 0800-059668 台南 0800-259988 花東 0800-056668 / 電子信箱:[email protected]
Epigenetic Proteins & Enzymes
全球知名⽣藥檢測服務⿓頭�Eurofins�Scientific�公司,提供⼀系列品質穩定的
表觀遺傳學重組蛋⽩及酵素。每批產品於出廠前皆經過嚴格控管以確保⾼純度® ®與⾼活性。可搭配�PerkinElmer�AlphaLISA �及�LANCE �Ultra�兩⼤技術平台,
進⾏藥物篩選實驗。
Epigenetic Proteins & Enzymes
l JMJD2A
l JMJD2B
l JMJD2C
l JMJD2D
l JMJD2E
l JMJD3
l hPHF8
l Sirtuin 1
l Sirtuin 2
l DNMT1
l DOT1L
l EHMT1
l EZH2
l G9a
l HAT1
l NSD1
l p300
l PRMT1
l PRMT3
l PRMT4
l SET7
l SET9
l SETD2
l SETD7
l SETD8
l SUV4-20H2
l NSD3 / WHSC1L1 (h)
l ASH1L
l ATAD2A
l ATAD2B
l BAZ2B
l BRD2(1)
l BRD2(2)
l BRD3(1)
l BRD3(2)
l BRD4(1)
l BRD4(2)
l BRDT(1)
l CECR2
l CREBBP
l FALZ
l KAT2A (GCN5L2)
l PB1(2)
l PB1(4)
l PB1A(3)
l PCAF
l PHIP(2)
l SMARCA2
l SMARCA4
l SP140
l TAF1(1)
l TAF1(2)
Readers (Bromodomain) Erasers
《 Epigenetics Proteins & Enzymes 產品項目列表 》
LIT. NO.: DM201521IMAGE: Enzo Life Sciences,
Eurofins Scintific, PerkinElmer
伯森生物科技股份㈲限公司Blossom Biotechnologies Inc.
台北 (02) 2788-2121 新竹 (037) 581-106 花東 0800-056668
台中 (04) 2323-3939 台南 (06) 235-6628 高雄 (07) 555-0764
Writers
Other Highly Active HDAC and Sirtuin Proteins
HDAC (rat liver)
HDAC1 (human), (recombinant) (His-tag)
HDAC2 (full-length) (human), (recombinant) (His-tag)
HDAC2 (human) (1-488), (recombinant) (His-tag)
HDAC3 (human), (recombinant) (His-tag)
HDAC3 / NCOR1 complex (human), (recombinant)
HDAC6 (human), (recombinant) (His-tag)
ALX-202-052
BML-SE456
BML-SE533
BML-SE500
BML-SE507
BML-SE515
BML-SE508
Product Name Cat. No.
HDAC8 (human), (recombinant)
HDAC10 (human) (recombinant) (Histag)
HDAC11 (human) (recombinant) (Histag)
SIRT1 (human), (recombinant) (His-tag)
SIRT2 (human), (recombinant) (His-tag)
SIRT3 (human), (recombinant) (His-tag)
SIRT5 (human), (recombinant) (His-tag)
Product Name
BML-SE145
BML-SE559
BML-SE560
BML-SE239
BML-SE251
BML-SE270
BML-SE555
Cat. No.