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DNA Nanostructures and Patterning . Taylor Stevenson Nucleic Acid Engineering February 15 th , 2011. Agenda. Structures Origami Branched Annealing Nanotubes Alternative Materials Patterning and Application Combing Origami as Scaffold Micropatterning Nanotubes. Origami Structures. - PowerPoint PPT Presentation
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DNA Nanostructures and Patterning
Taylor StevensonNucleic Acid Engineering
February 15th, 2011
Agenda
• Structures – Origami – Branched Annealing– Nanotubes– Alternative Materials
• Patterning and Application– Combing– Origami as Scaffold– Micropatterning Nanotubes
Origami Structures
Rothemund “Folding DNA to create nano-scale shapes and patterns” Nature Articles (2006)
Origami Structures
Rothemund “Folding DNA to create nano-scale shapes and patterns” Nature Articles (2006)
Branched Annealing Structures
Modified from Luo et.al. Nature Letters 2006 5.
Nano-Tube Structures
Chen et. al. “Approaching The Limit: Can One DNA Oligonucleotide Assemble into Large Nanostructures?” (2006)
Other Forms of Nucleic Acid
Braasch & Corey, Chemistry and Biology (2001)
Other Forms of Nucleic Acid
Lin et. al. “Mirror Image DNA Nanostructures forChiral Supramolecular Assemblies” Nano Letters (2009)
Combing Linear DNA for Nanowires
Deng and Mao “DNA-Templated Fabrication of 1DParallel and 2D Crossed Metallic
Nanowire Arrays” Nano Letters (2003)
DNA Origami as a Scaffold
•RNA/DNA hybridization assays
Ke et. al. “Self-Assembled Water-Soluble Nucleic Acid Probe Tiles for Label-Free RNA Hybridization Assays”
Science (2008)
DNA Origami as a Scaffold
Ke et. al. “Self-Assembled Water-Soluble Nucleic Acid Probe Tiles for Label-Free RNA Hybridization Assays”
Science (2008)
DNA Origami as a Scaffold
Rinker et. al. “Self-assembled DNA nanostructuresfor distance-dependent multivalentligand–protein binding” Nature Letters (2008)
Micropatterning Nanotubes
Lin et. al. “Functional DNA Nanotube Arrays: Bottom-Up Meets Top-Down” Ange Chem (2007)
2D Patterning Branched Annealing Structures
2D Patterning Branched Annealing Structures
• Rigidity of “monomers” affects 2/3D structure.
• Varying the length of red region produces different flexibilities.
Stiffness
Flexibility
2D Patterning Branched Annealing Structures
Stiffness
Flexibility
2D Patterning Branched Annealing Structures
Micropatterning Nanotubes