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DNA
The Genetic Material
The genetic material must meet 4 criteria 1. Information: 2. Transmission: 3. Replication: 4. Variation:
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IDENTIFICATION OF DNA AS THE GENETIC MATERIAL
Two strains of Streptococcus pneumoniae S Smooth
Secrete a polysaccharide capsule Produce smooth colonies on solid media virulent
R Rough Unable to secrete a capsule Produce colonies with a rough appearance avirulent
Two coat types II III
Four possible phenotypes IIS or IIIS or IIR or IIIR
1928 - Frederick Griffith’s Transformation Experiments
Griffith’s Experiments
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Figure 9.1
Rare mutations convert strains S into R strain & vice versa
But mutations do not change coat types II into III or vice versa
9-8Figure 9.2
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Something from the dead type IIIS transformed type IIR into type IIIS
Called this process transformation
The unknown substance was termed the transforming principle
Griffith’s Conclusions
Griffith’s transforming principle was the genetic material
Transformation assay to identify actual biomolecule
Major constituents - DNA, RNA, proteins, carbohydrates, & lipids
Made cell extracts from type IIIS cells containing each of these macromolecules
1944 - Avery, MacLeod & McCarty Identify the Genetic Material
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9-11Figure 9.3
Mixed each extract with type IIR cells to test for transformation Only extract containing purified DNA transformed type IIR to type IIIS Verify that DNA, not RNA or protein, is the genetic material
Avery’s Experiments
1952- Hershey & Chase Confirm DNA is Genetic Material Using Phage T2
Figure 9.5
Life cycle of phage T2
Phage Attached to E. coli Cell
The Hershey and Chase experiment:
Used radioisotopes to distinguish DNA from proteins 32P labels DNA specifically 35S labels protein specifically
Infect non-radioactive E. coli with radioactively-labeled phages
Remove phage coats from cells Is 32P or 35S inside bacteria?
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Hershey & Chase Experiment
S
Hershey & Chase Experiment
The Data
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9-20Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
In 1956, A. Gierer and G. Schramm isolated RNA from the tobacco mosaic virus (TMV)
Purified TMV RNA caused the same lesions as intact TMV viruses Therefore, the viral genome is composed of RNA
Since that time, many RNA viruses have been found
RNA is Genetic Material in Some Viruses
9-21Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display