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Dr. Mariam Eshak 2015
Expression of Inflammatory and Cell Death Genes and DNA Damage Induced by Endotoxic Shock in Laying Hens
Mariam G. Eshak1, Ahmed Abbas2, M. I. El-Sabry2 and M. M. Mashaly2,3
1Department of Cell Biology, National Research centre, Giza, Egypt. 2Animal production Department, Cairo University, Giza, Egypt. 3Animal and Poultry Science Department, Pennsylvania State University, USA.
Title
Dr. Mariam Eshak 2015
Low egg production
High Mortality ?
How
ObjectiveThis investigation was conducted to determine the physiological response and evaluate the expression of inflammatory and cell death genes and DNA damage induced by endotoxic shock in laying hens.
Challenge
Dr. Mariam Eshak 2015
Experimental Design
Control E. Coli
120 birds 120 birds
(107 c
olon
ies/
shut
)
35 wk of age
40 40 40
H&N
Gene expression analysis Real Time PCR
DNA damage Comet assay
40 40 40
Dr. Mariam Eshak 2015
Results
Dr. Mariam Eshak 2015
Treatment Tissues Number of animals
No. of cells Class of comet
DNA damaged cells )%(
Analyzed Total comets 0 1 2 3
Control Brain 5 500 21 479
16 5 0 4.2
E.coli 5 500 43 45
7 11 14 18 8.6
Control Liver 5 500 23 477
19 4 0 4.6
E.coli 5 500 56 44
4 10 22 24 11.2
Stages of DNA Damage
0 1 2 3
Conclusion Introducing E. Coli to hens induced inflamm-atory physiological response and triggers cell death program by promoting P38, IL-1β, and TNF-α gene expression in the brain and liver.
Moreover, The DNA damage % was higher in liver more than brain tissue
