N A M A S K A RN A M A S K A R

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STATUS OF BIOTECHNOLOGY IN

LIVESTOCK SECTOR, NEPAL

Dr. Suderson Prasad Gautam

Chief, Central Biological Production Laboratory Nepal

In Livestock Sector BIOTECHNOLOGY In Livestock Sector BIOTECHNOLOGY and its TOOLS are in use for long time and its TOOLS are in use for long time

in Nepalin Nepal

Animal Health

�Disease diagnosis

Animal Production

�Genetic Improvement �Disease diagnosis

�Disease control

�Disease monitoring

�Preservation of organisms/cells /tissue cultures/

�Genetic Improvement

�Feed Source

�Sterile Parasitic Cyst feeding

Animal Health: Animal Health: Disease DiagnosisUse of biotechnological tools in diagnosis of Bacterial, Viral and Parasitic diseases

•Animal Tissue Culture

•ELISA/ PCR /Animal/Embryo inoculation /HA/HI

•Diagnostic kit production (Imported)

•Polyclonal Sera production and purification (Private+Govt.)•Polyclonal Sera production and purification (Private+Govt.)

Animal Health: Animal Health: Disease Control

Vaccine Production

• Viral

• Bacterial

• Parasitic (Imported)• Parasitic (Imported)

Vaccine Production and Titration test

BacterialH.S., B.Q., Anthrax, (Fermentation Technology, H.A./H.I.)

Fermentation Tank For Vaccine ProductionAt Central Biological Production Laboratory,Tripureswor, A Government Institution

Viral Vaccine

PEST DES PETITS RUMINANATS (PPR)Nepal was the First country to produced PPR in south east Asia

Tissue Culture vaccine in CBPL Tissue Culture vaccine in CBPL and Rabies Vaccine Lab. (Govt.)

Field Trial : Local and cross breeds Cattles in Government (NARC) farm in Khumaltar, of

Kathmandu valley.

Field Trial :Buffalo calves in the Lampatan Government farm, Pokhara

Field Trial Conti….Field Trial Conti….

Field trial of ND I2 VaccineField trial of ND I2 Vaccine

Disease/vaccine monitoring and sera production

Animal InoculationAnimal Inoculation

• Hyper Immune serum production in rabbits by repeated immunization using HS Aerosol B 3,4 antigens

• Preliminary Indirect Haemagglutination Haemagglutination (IHA) test of the serum samples collected

Preservation of organisms and cellsPreservation of organisms and cells

Lyophilization

Facilities In CBPL

Animal ProductionAnimal Production

• Genetic Material Transfer1. AI/Embryo Transfer (Govt.)2. Productive Breeds of Broilers (Imported)3. Animal feed improvement (Soya-GMO)3. Animal feed improvement (Soya-GMO)

Transfer of genetic materials in animal

AI Farm , Pokhara, A Govt. Institution

BULL : PJE-3 SHYAM BREED : JERSEY 100%

Embryo Transferred BORN BULL

Viral Culture in Embryonated Eggs

Strength of these institutions using Strength of these institutions using biotechnology and its tool in diagnosis/ biotechnology and its tool in diagnosis/

production/ researchproduction/ research• Capable in Controlling the contagious and

infectious disease of animals and man• Safeguarding the health and the economy • Helping to establish big industries and

employment opportunitiesemployment opportunities• Helping in early detection of the diseases that

can be controlled in initial stage• Easy improvement in genetic material and

production capabilities as well as other research • And Much more…

WeaknessWeakness of these institutions using of these institutions using biotechnology and its tool in diagnosis/ biotechnology and its tool in diagnosis/

production/ researchproduction/ research• Main involvement of govt. sector but needs participation

of private sectors and teaching institutions• Budgetary constraints: Govt. always in financial crisis

and cuts the Budget in spite of inflation • Technology improvement is a continuous process and

there is always opportunity to improve the • Technology improvement is a continuous process and

there is always opportunity to improve the skill/knowledge transferred before but in the govt. no such opportunity are available

• Technical manpower involved in this sector is very less • The machine/equipment required for these works are

highly technical and specific but there is no/ less personnel for maintenance and repair

• Great opportunities for the private sector to involve in the production and research sector as the livestock and its products are in high demand

• Much more sector are there for research and

Opportunities of these institutions using Opportunities of these institutions using biotechnology and its tool in diagnosis/ biotechnology and its tool in diagnosis/

production/ researchproduction/ research

• Much more sector are there for research and production in biotechnological / development-repair and maintenance manpower consumption

• Institution should be opened for manpower development for national and international consumption as it is in high demand

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Thank you