Upload
others
View
2
Download
0
Embed Size (px)
Citation preview
E. motion = E. coli + Motion
We will further familiarize people with iGEM and synthetic biology. We are preparing two amazing plans for educational activities for KIT-Kyoto 2015.
8 Future Plan
1. We conducted a large-scale survey on genetic engineering (n = 1571).2. We constructed new devices that produce monoterpene in E. coli and yeast cells.3. More than 600 people experienced BioArt and genetic engineering through our activities.
7 Conclusion
6 Policy & Practices
Our activities were broadcast on evening news by a Japanese TV station.
Our team members gave lectures on genetic engineering at middle and high schools.
Heated discussion for hours to decide what we should do to raise the awareness of genetic engineering.
We tweeted to communicate with other iGEM teams.
We collaborated with other iGEM teams such as Imperial, Linkoping Sweden, ETH Zurich and Colombia.
We launched wiki specialized for iPhones and Androids.
Hundreds of students enjoyed creating their own artworks with E. motion.
We gave presentations about genetic engineering and iGEM to high school students and their guardians.
The large-scale survey was conducted in the downtown Kyoto and at our institute.
We designed E. coli and yeast cells which synthesize aromatic substances in orange and lemon such as limonene, pinene and terpinene.We successfully produced γ-terpinene in yeast cells.
Scent5
LIMS: d-limonene synthase, about 67kDaγTPNS: γ-terpinene synthase, about 65kDa BPINS: β-pinene synthase, about 65kDa GST: GST tag protein, about 27kDa
W e u s e d S P M E ( S O L I D P H A S E M I C R O E X T R A C T I O N ) t o c o l l e c t γ-terpinene at the headspace of the cul ture. However , any amounts of γ-terpinene were not detected at the headspace of the cu l ture (30-min exposure time). Next we collect γ-terpinene in the liquid phase of the culture using monotrap (1-h exposure t ime) and an amount o f γ-terpinene was detected in the liquid phase of the culture.
In 2010, team KIT-Kyoto produced E. coli expressing fluorescent proteins. Hundreds of students experienced BioArt in our institute.
3 Conventional BioArt
Spider Lily
Harvest Moon
Rabbits on the Moon
Larvae's motion is guided by monoterpene, yet it is art of chance unlike any other.
4 Motion
We adopted BioArt as a powerful tool to familiarize people with genetic engineering. We added MOTION and scent to the conventional BioArt and took it to the next level. E. motion is the BioArt with dynamics and it inspires not only your visual sense but also your olfactory perception. E. motion shakes your emotion!
2 E. motion
Conventional BioArt
MotionScent
NextGeneration
BioArt
Our survey disappointingly indicates that more than 70% of Japanese people think GM foods are enigmatic and have bad images on genetic engineering.
We conducted a large-scale survey on Japanese people's attitudes toward genetic engineering.(n = 1571).
Introduction1
What do you think about genetic engineering?
Positive
Somewhat positive
Somewhat negativeNegative
Not sure
What kind of image do you have on genetic engineering?
Good
Bad
I don't care
Do you feel familiar with genetic engineering?
Yes
No
When you go to a grocery store,which do you buy, GM foods or non-GM foods?
Prefer non-GM foods
Always non-GM foods
Always GM foods
No response
Prefer GM foods
If the price of non-GM foods is 10-20% more expensive than GM foods, what would you do?
Still buy non-GM foods
Buy GM foods
Other
When you hear genetic modification and biotechnology, what comes to your mind? Please select all that apply.
GM foodsCloned animal
iPS cells
Biofuel
Artificial lifeGene therapy
BioethicsBioweapon
ES cells
Other
No response
Which sentence describes your ideas about genetic modification?Please select all that apply.
Unproven safetyPotential effects on future generations
Mysterious
Potential effects on ecosystem
UnethicalNo negative effects on future generations
No response
Other
Do you think that the research of genetic modification should be continued in the future?
Yes
No
Neither of the above
200 larvae were placed at the center of an agar plate (2.0%, radius: 8 cm). A piece of filter paper was placed on each end of the plate (Fig.1). 2.5 µl of monoterpene was dropped on one side of the filter paper (Monoterpene) and cover the plate with a lid. Three minutes later, the number of larvae within a 3 cm radius from the filter paper was counted (A or B) . The concentrations of monoterpene are diluted by 20% and 10% with DMSO. The control is DMSO.
Western blot data (Fig.2) showed clear bands around 90kDa in the lanes of p427-TEF- monoterpenoid synthase but not in the lane of empty vector.
monoterpene
pGEX
Non-mevalonatepathway
IPPDMAPP
pTEF
IPP DMAPP
GPP
Fig.2
GST CuMTS E2/3/62
p427-TEF
KpnⅠ
SpeⅠ XhoⅠ
BamHⅠEcoRⅠSmaⅠSalⅠ
2μ
kanMX
CYC1tTEFp
Ampr
In Yeast
In E. coli
TEF promoter
TAC promoter
TEF
GEX
Cyc1
RBS
Mono-terpenesynthase
Mono-terpenesynthase
Eww...
What should we do?
Our solution is the education through BioArt.
1. We will hold the National BioArt competition in Japan to spread BioArt.
2. We will shoot movies with E. motion and show them to the general public.
Fig.1 Measuring apparatus
AMonoterpene
BControl
Mevalonatepathway
O P O P O-
OO
O-O-
Extra GPP should be added in the medium.
100% Monoterpene Assay
Monoterpene Control
Supported by・Mr. Teppei Hirayabu, advisor of team KIT-Kyoto iGEM 2010
・Dr. Takehiko Shimada, National Agriculture and Food Research Organization in Japan
・COSMO BIO
E. motionIGEM 2014
E.MOTION
- Dawn of the New Generation BioArt -
OW!
3. Laboratory environment will surely improve with the smell of citrus which is famous for aromatics.