Microfluidic Well/Channel Design● Well design for protein (from volumes & concentrations)

○ Volume: 250uL■ Selected based on recommended working volume of Corning for 96 well

plate (75-200uL), but also considering volumes large enough for device to handle

○ Diameter of well: 7mm■ Selected based on diameter of standard 96 well plate commonly used in

fluorescent plate readers○ Height of well: 6.5mm

■ Calculated using equation:● V= pi*r^2*h● h=V/pi*r^2

● Well design for reagent○ Volume: 1000uL

■ Selected as a volume greater than that required for a single protein well■ Volume would be ideal for multiple wells with protein, act as a main

reservoir to lead out to additional wells○ Diameter of well: 10mm

■ Selected to keep increase of height of well minimal while increasing the volume the well holds with respect to the protein well

○ Height of well: 12.7mm ■ Calculated with equation used in protein well design

● Integration of solenoid valve○ Design of channels to incorporate valve

■ Narrow channels of minimum height and width for valve■ Length of channel (both inlet and outlet)- 10mm■ Must consider device’s ability to print

○ Consider height and width of valve to fit appropriately into PDMS● Hand drawn design

○ Image 1

*Channel and valve measurements no longer valid, need to incorporate new valve○ Image 2

● PDMS design

○ Using Silhouette Studio - craft cutting device○ Print in individual layers, use plasma wand to polarize surfaces, stick together,

heat at approximately 80 degrees Celsius to strengthen bonds○ Layer thicknesses available: 100um, 300um, 600um

● Next Steps○ Re-design wells to fit most up to date valve design○ Make prototype of PDMS wells and channels