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Effect of Thawing Temperature and Time onPost Thaw Sperm Motility of Cryopreserved

Jacks Semen

R.A. Legha, A. Arangasamy, Yash Pal, R. C.

Sharma and S. N. Tandon

Equine Production Campus

National Research Centre on Equines,Bikaner 334 001 (Rajasthan).

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Equines hold special position in livestockboth for civil and military purpose in view ofits multifaceted utility.

AI with frozen Jacks semen is animportant aspect in production of superiormules and up gradation of indigenous

donkeys. Various studies in other species indicatedthat thawing temperature and durationplays significant role in post-thaw motility.

Keeping in view, a trial was conducted ondifferent thawing temperature and timeduration to study its effect on post thawsperm motility.

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Five exotic jacks maintained at Equine

Production Campus, NRCE Bikaner wereutilized for semen collection and itspreservation.

Citrate- EDTA and Lactose glucose-EDTAEgg yolk were used as primary andsecondary extenders, respectively forsemen cryopreservation.

The semen was stored in 0.5 ml capacitystraws.

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Semen processing & freezing

Semen collection

Gel removal

Microscopic and physical examinations

Adding media and Centrifugation

Decanting supernatant and adding freezingmedia

Filling and sealing of straws with cooled

semen Freezing in planner

Plunging in LN2 for storage

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Post Thaw Sperm Motility at DifferentTemperatures and Duration

Thawing

Temperature

Duration Post-thaw sperm

Motility (%)

37C 30 sec 43.482.07

37C 1 min 51.401.3137C 2 min 47.801.13

50C 15 sec 32.600.94

50C 30 sec 23.201.05

50C 1 min 18.600.87

70C 5 sec 44.401.03

70C 15 sec 17.400.89

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It is concluded that the optimum temperaturefor thawing of semen straws was observed as37 C for one minute.

However, straws can also be thawedsatisfactorily at 37 C for two minutes and 70 Cfor 5 seconds only.

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