8/2/2019 Effects of Exercise on the Iso Electric Patterns

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AuthorLamon, Severine; Martin, Laurent; Robinson, Neil; Saugy, Martial,Ceaurriz, Jacques de and Lasne, Francoise

Title Effects of exercise on the isoelectric patterns of erythropoietin.Source Clinical Journal of Sport Medicine. Vol. 19(4): 311-315 July 2009

Abstract

Objectives: Recombinant erythropoietin has a strong impact onaerobic power and is therefore one of the most potent doping agentsin endurance sports. The anti-doping control of this synthetic hormonerelies on the detection, in the urine, of its isoelectric pattern, whichdiffers from that of the corresponding natural hormone, the latter beingtypically more acidic than the former. However, a small number ofnatural urinary patterns, referred to as "atypical patterns," are lessacidic than the dominant form. Based on anecdotal evidence, the

occurrence of such patterns seems to be related to particularstrenuous exercises. This study aimed to demonstrate this relationusing a strenuous exercise protocol. Design: Seven athletes took partin a training protocol including a series of supramaximal short-durationexercises. Urine and blood samples were collected throughout theprotocols. Settings: World Cycling Center, Aigle, Switzerland, andresearch laboratories. Participants: Seven top-level athletes (cyclists)were involved in this study. Main Outcome Measures: Erythropoietin(EPO) isoelectric patterns were obtained by submitting blood andurine samples to isoelectric focusing. Additional protein dosages wereperformed. Results: Supramaximal short-duration exercises induced

the transformation of typical urinary natural EPO patterns into atypicalones. None of the obtained atypical patterns fulfilled the 3 criteriamandatory for reporting an adverse analytical finding. Serum EPOpatterns were not affected by the exercises that caused thetransformation of urinary patterns. Conclusion: An exercise-inducedtransient renal dysfunction is proposed as a hypothetic explanation forthese observations that rely on parallel investigations of proteinuria inthe same samples

Author Mamoun, L.; Simar, D.; Caillaud, C.; Coste, O.; Barbotte, E.;Peruchon, E.; Rossi, M. and Mariano-Goulart, D.

TitleResponse of calciotropic hormones and bone turnover to briskwalking according to age and fitness level

SourceJournal of Science and Medicine in Sport. Vol. 12(4):463-467, Jul.2009

Abstract

Ageing is associated with a gradual bone loss and physical activityhas been suggested as practical strategy for a non-pharmacologicalprevention of osteoporosis. However, until now, the specificmechanism by which physical activity affects bone tissue is not

thoroughly understood. The aim of this study was to evaluate theeffect of strenuous exercise on bone metabolism as a function of age