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Enantiomers of Lipoic Acid Differentially Affect Nrf2 Activation. Kelly Crotty Dr. Tory Hagen. Our bodies are under attack from both external and internal toxins. Nrf2: A Transcription Factor. B zip. Transcription factor Binds to the A ntioxidant R esponse E lement (ARE). - PowerPoint PPT Presentation
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•Transcription factor
• Binds to the Antioxidant Response Element (ARE)
•Influences over 200 genes with the ARE
•These genes produce detoxification enzymes and antioxidant proteins
Nrf2: A Transcription Factor
B zip
• Always being produced
• Almost immediately bound to the protein Keap1 and degraded
Nucleus
Cell
Nrf2 Production and Degradation
Nucleus
Cell
Nrf2 Activation
• Under stress, Nrf2 returns to the nucleus in greater
amounts to stimulate gene expression.
How potent is S-LA compared to the commercially available racemic mixture or natural R-LA?
Question:
Methods:
Measure relative Nrf2 protein levels in the nucleus through western blot analysis
Isolate the nuclei of cells that have been treated with lipoic acid
R-LA > R,S-LA > S-LA
in activating Nrf2
Implications:
Lipoic
Acid •Though R-LA and S-LA have similar
structure, they activate Nrf2 at
completely different concentrations
Future Projects:
• Continue the concentration curve until R,S-LA and S-LA
have also reached saturation
• Add more time points for the time course
• Repeat the time course with R-LA