ESA Reno Nevada 2008

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    Efficacy of Flameless Catalytic InfraredRadiation Energy Against Different Life

    Stages of Insects

    Khamis Moses, Bhadriraju Subramanyam, Dogan Hulya and

    Gwirtz JeffDepartment of Grain Science and Industry

    Kansas State University

    Manhattan, KS 66506

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    Introduction

    Hypothesis: How do different ages of stored productinsects respond to flameless catalytic infraredradiation?

    Objective 1: Age Grading of internal insects usingFaxitron and X-ray microtomography

    Materials and method

    Results

    Objective 2: Infrared treatment

    Materials and method

    Results

    Conclusion

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    Infrared Energy Electromagnetic spectrum; radiation energy with

    wavelength longer than visible light but shorter thanmicrowave Water molecules absorb highest mid-infrared (2.8 m

    and 7 m) energy

    Differential heating: insects vs grain

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    Current Pest Management Problems Ban on organophosphates pesticides (phosphine)

    Development of insect resistance to some of theavailable pesticides

    Pesticides residues in foods

    Lack of international consensus on some pesticidesuse

    Ineffectiveness of some pesticides against certainlife stages of insects

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    Previous research

    Old infrared heaters used natural gas or propane gas

    combusted over ceramic panels with temperaturesclose to 926C

    Such high temperatures are unsafe for grain handlingfacilities

    No sufficient research to determine how thedifferent insects ages were affected by infraredradiation

    Banjo thermometer was used to read graintemperature.

    Wheat, wheat product qualities were not evaluated

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    Infrared for stored grain insect control

    Flameless infrared energy is a

    green

    technology,there are no regulated emissions, only products arewater, heat and carbon dioxide

    Kills external and internal stored-grain insects

    Kills microorganisms

    It is a rapid method (insects are killed in less than60s)

    Flameless catalytic infrared heater is cheaper

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    Research objectives Determine factors affecting efficacy of infrared

    radiation against eggs, larvae, pupae, and adults ofthree stored-grain insect species

    Evaluate effects of infrared radiation on wheatgermination

    Evaluate effects of infrared on mold counts

    Evaluate effect of infrared radiation on quality of

    wheat and wheat flour and products made frominfrared-exposed grain

    Conduct an economic analysis of treatmenteffectiveness

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    Age grading

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    Pupae of Lesser grain borer

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    Tunnel width (mm)Lesser grain borer Rice weevil

    __________________________________

    Age (Days)

    7 0.24 0.03* 0.26 0.0114 0.34 0.01 0.57 0.0321 0.40 0.02 0.72 0.0124 0.53 0.0 1.39 0.0128 0.61 0.02 Emerged

    * Observed on the eighth and ninth day

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    X-ray microtomography Skyscan

    Source: www.microphotonic.com

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    Pupae of LGB with XMT

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    Pupae of RW with XMT

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    Development time for LGB and RW

    Number of Days

    20 22 24 26 28 30 32 34 36

    N

    umberofAdults

    Emerged

    0

    10

    20

    30

    40

    50

    60

    70

    Rice weevil

    Lesser grain borer

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    Factors evaluated where;-

    Different insects ages (Eggs to adults)

    Distance of grain from surface heater, (8.0 and 12.7cm)

    Quantities of grains (113.5 and 227.0 g)

    Exposure time (45 and 60 seconds)

    Infrared treatment and insects mortality

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    Bench top infrared heater

    Materials and methods

    A Company based in Independence KS;www.catalticdrying.comdesigns commercial scaleflameless catalytic heaters for specific uses

    http://www.catalticdrying.com/http://www.catalticdrying.com/
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    Typical Temperature Profile

    Temperature vs Time (45s)

    Time (s)

    0 10 20 30 40 50

    Temperature(0C)

    40

    60

    80

    100

    120

    140

    T1D1W1

    T1D1W2

    T1D2W1

    T1D2W2

    Temperature vs Time (60s)

    Time (s)

    0 10 20 30 40 50 60

    Temperature(0C)

    40

    60

    80

    100

    120

    140T2D1W1

    T2D1W2

    T2D2W1

    T2D2W2

    d l h d f

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    Mean no. adults that emerged fromcontrol

    Age (days) Grain qty (g) R. dominica T. castaneum S. oryzae

    Mean SE Mean SE Mean SE

    0 113.5 443 32.6 43 5.9 271 18.0

    0 227 582 13.5 49 3.4 340 28.2

    7 113.5 241 22.5 100 1.0 221 15.8

    7 227 541 24.1 101 1.0 347 21.714 113.5 302 22.4 100 2.0 254 13.8

    14 227 502 17.2 98 4.0 336 23.4

    21 113.5 145 52.2 100 0.3 225 22.6

    21 113.5 240 33.2 103 1.0 381 17.4

    24 113.5 231 62.1 99 1.0 249 46.7

    24 227 451 40.0 102 2.0 374 11.9

    28 113.5 256 51.2 360 31.0

    28 227 490 11.9 412 15.6

    ** n =3

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    Mortality for all ages

    0.0

    0.2

    0.4

    0.6

    0.8

    1.0

    75

    8085

    9095

    100105

    110115

    0

    10

    20

    30

    40

    ProbabilityofDeath

    Temp

    eratur

    e(0C)

    Age(Days)

    Lesser Grain Borer

    0.2

    0.4

    0.6

    0.8

    1.0

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    Factors and their interactions

    Effect DF ChiSq Pr>ChiSq_______________________________________________

    Age 6 642.6

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    Odds of deathLesser grain borer

    Age (Days)

    0 10 20 30 40

    OddsRatios

    0

    1

    2

    3

    4

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    0.0

    0.2

    0.4

    0.6

    0.8

    1.0

    75

    8085

    9095

    100105

    110115

    0

    510

    1520

    25

    ProbabilityofDea

    th

    Temp

    eratur

    e(0C)

    Age(Days)

    Red Flour Beetle

    0.2

    0.4

    0.6

    0.8

    1.0

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    Red flour beetle

    Effect DF ChiSq Pr>ChiSq____________________________________________

    Age 5 26.7

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    Red flour beetle

    Age (Days)

    0 5 10 15 20 25 30

    OddsRatio

    0.0

    0.5

    1.0

    1.5

    2.0

    2.5

    3.0

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    0.0

    0.2

    0.4

    0.6

    0.8

    1.0

    7580

    8590

    95100

    105110

    115

    0

    10

    20

    30

    40

    ProbabilityofD

    eath

    Temp

    eratur

    e(

    0C)

    Age(Days)

    Rice Weevil

    0.2

    0.4

    0.6

    0.8

    1.0

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    Rice weevil

    Effect DF ChiSq Pr>ChiSq_______________________________________________

    Age 6 1404.5

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    Rice weevil

    Age (Days)

    0 10 20 30 40 50

    OddsRa

    tio

    0.0

    0.2

    0.4

    0.6

    0.8

    1.0

    1.2

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    Insects mortality

    It is a function of temperature

    Longer treatment time, shorter distance and smallquantity of grain all influenced the attained temperatureand mortality

    Overall (by species), lesser grain borerwas the mosttolerant to infrared treatment, followed by, red flour

    beetle then rice weevil Eggs of rice weevil were the most tolerant to infraredradiation

    Old larvae of all species more tolerant to infrared energythan young ones

    Pharate adults of lesser grain borer were more resistantthan the adults

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    Acknowledgement

    Research was funded by USDA/CSREES-NC-IPMgrant

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    Thank You