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― 40 ―
Head
Experimental Animal Division
Goal
Programs
The Experimental Animal Division has two missions: (a) to
collect, preserve, conduct quality control of and distribute
high-quality mouse resources, and (b) to develop novel mouse
models and useful technologies for bioresource activities. Our
ultimate goal is to contribute to human welfare by facilitating
research in life sciences with our mouse resources. The mouse
is one of the most superior model organisms used in life
sciences to understand human health and overcome diseases.
Since 2002, RIKEN BRC has been designated as the central
core facility for mouse resources in Japan by the National
BioResource Project (NBRP) of MEXT. With support from
the scientific community, RIKEN BRC has collected over
3,200 mouse strains including genetically modified models,
ENU mutants, and inbred and wild-derived strains. RIKEN
BRC is a founding member of the Federation of International
Mouse Resources (FIMRe) together with The Jackson
Bioresource program: Collection, preservation, quality-
control and distribution of the mouse resources
MembersSenior Scientist, Head of Experimental Animal Division
Atsushi YOSHIKI, Ph.D. (2004.12~)
Senior Research Scientist
Fumio IKE, Ph.D. (2001.4~)
Senior Technical Scientist
Noriko HIRAIWA (2001.4~) Hatsumi NAKATA, Ph.D. (2007.4~)
Research Scientist
Kazuyuki MEKADA, Ph.D. (2003.8~) Yasuyuki KITAURA, Ph.D. (2006.3~)
Technical Staff Ⅱ
Ayumi MURAKAMI (2001.4~) Maiko IJUIN (2004.10~)
Masayo KADOTA (2006.4~) Mika OKAWA (2006.4~2008.1)
Assistant
Norie TSUDA (2001.12~2007.3)
Atsushi YOSHIKI, Ph.D.
Laboratory, European Mouse Mutant Archive (EMMA)
and other centers, and has participated in the International
Mouse Strain Resource (IMSR) to distribute mouse strains
worldwide. To meet research and social needs, our mice are
cleaned up to a specific pathogen-free state, strictly monitored
of their health, and accurately tested on their genetic
modifications and backgrounds. Phenotypic information of
the strains is frequently updated to enrich their value. With
these activities, RIKEN BRC plans to build mouse resources
that meet the highest global standards by 2010. The Material
Transfer Agreement (MTA) is used to protect the intellectual
property rights of the developer of the strains. Training
courses concerning advanced technologies are provided for
users to best use our resources. RIKEN BRC collaborates
with Asian and other overseas institutes to contribute to the
advancement of life sciences in the world.
I. Development program: Development of novel mouse
models and technologies necessary for program I.
II.
Experimental Animal DivisionRIKEN BRC Annual Report 2005 ~ 2007
― 41 ―
Student Trainee
Jan, MEI-LING (2007.4~)
Visiting Scientist
Chikako YOSHIDA-NORO, Ph.D. (2005.4~) Ikuko SAKAI (2005.8~)
Chino SASAOKA (2005.8~) Ryoji HYODO (2006.11~)
Nobuhiko ONDA (2005.8~2007.3) Kaori HIGUCHI (2006.11~2008.3)
Kayuri MURAKI (2006.11~2008.3)
Visiting Technician
Ayako KAJITA (2006.5~)
Agency Staff
Fujimi ARAI (2001.4~) Megumi KOBAYASHI (2001.4~)
Reiko KOJIMA (2001.4~) Akemi KOSHIYAMA (2001.4~)
Kyoko MEKADA (2001.4~) Yukiko SEKI (2001.4~)
Aya UEKI (2001.4~) Masaru ONUMA (2001.5~)
Atsushi CHOEI (2001.6~) Rika TAKASHIMA (2001.6~)
Teruo SAITO (2004.12~) Miki NAKAYAMA (2005.5~)
Chihiro YOKOYAMA (2005.10~) Kinuko ITO (2005.10~)
Yusuke OBA (2005.12~) Reiko KAWAI (2006.1~)
Hitomi TAKAHASHI (2006.4~) Masashi YOKOTA (2006.4~)
Chiharu OKUBO (2006.5~) Akemi YASUI (2006.5~)
Masashi OZAWA (2006.6~) Masako KITO (2006.9~)
Hiroyuki OKAMOTO (2006.9~) Tomomi HASHIMOTO (2007.4~)
Makiko IIZUMI (2007.4~) Naoki OTAKA (2007.4~)
Chieko YAMADA (2007.4~) Tomoe SAKAI (2007.4~)
Maki YAMAMOTO (2007.6~) Miki NAKAYAMA (2007.11~)
Chie FUJIMOTO (2008.4~) Aya MIMORI (2008.4~)
Kyuichi TAGUMA (2008.4~) Tomoko TAKAHASHI (2008.4~)
Naoki HIRANO (2008.6~) Hiromi HISAMATSU (2007.6~)
Fumie MIYAZAKI (2001.4~2008.3) Masami ICHIKAWA (2007.3~2008.3)
Hitomi OKANO (2007.3~2008.3) Sanae MIYAMOTO (2006.11~2007.3)
Masayuki SAITO (2006.10~2007.11) Satoe NAKAMURA (2005.4~2008.3)
Mika KOMURO (2005.6~2007.2) Yoko ONUKI (2005.10~2007.10)
Chie HOSHI (2005.11~2007.11) Naomi ISO (2006.2~2007.3)
Junichi MASUDA (2006.3~2008.7) Emiko FUKUDA (2006.4~2008.3)
Yasuko NISHIKAWA (2006.4~2007.9) Ayako KAJITA (2006.5~2007.3)
Katsumi WATANABE (2007.11~2008.5) Yuko FUJIMOTO (2001.4~2007.3)
Contract Staff
Noriko KATAOKA (2001.4~) Tomohiro OKUBO (2002.2~)
Keiko TOMIYAMA (2002.3~) Noriko NOGUCHI (2004.1~)
Kunihiro SAKURAI (2005.2~) Mariko HASEGAWA (2006.3~)
Takeshi MATSUZAKI (2006.3~) Katsuhiko YAGISHITA (2006.7~)
Takashi FURUYA (2007.2~) Takayoshi KOSHIDO (2007.4~)
Takeshi NAGAO (2007.4~) Masaki YAMAGUCHI (2007.4~)
Kiyohiko ASANO (2007.8~) Masataka ASANO (2007.12~)
Experimental Animal DivisionRIKEN BRC Annual Report 2005 ~ 2007
― 42 ―
Programs
1. Bioresource Program
(1) Collection, preservation and distribution of mouse
strains
This program has been operated in cooperation with research
scientists, technical scientists, technical staffs, agency and
contract staffs. In regard to the annual plan for the operation
of the Experimental Animal Division, we have obtained
useful advice and suggestions from distinguished members
of the BRC Experimental Animal Steering Committee, BRC
Advisory Council, Promotion Advisor and RIKEN Advisory
Council. MTAs for the deposition and distribution of
mouse resources have been used to protect the intellectual
property right of the Developer, and to clarify the terms and
conditions of use for the Recipient of the biological resources,
respectively. We have collected over 3,200 strains mainly
developed in Japan, such as inbred, transgenic, knockout, Cre-
driver, ENU mutant and wild-derived strains, as well as gene-
trap ES clones. These strains are useful models for the study
of cancer, immunity and allergy, endocrine diseases, brain
and neurological disorders, development and differentiation
abnormalities, and sensory organ abnormalities (Fig.1).
Okamoto, Hirano, Koshido, Ike, Yoshiki, Asano(T), Matsuzaki, KitauraAsano(K), Sakurai, Yamaguchi, Saito, Okubo(T), Yokota, Masuda, Taguma, Furuya
Mitsunari, Hasegawa, Kojima, Arai, Oba, Nagao, Onuma, Otaka, Choei, Takahashi(H)Yamamoto, Hashimoto, Iizumi, Kawai, Nakayama, Hisamatsu, Kajita, Okubo(C), Nakayama, Fujisawa
Kobayashi, Sato, Mimori, Sakai, Takahashi(T), Ito, Koshiyama, Ozawa, TakanoOkada, Murakami, Shima, Fujibayashi, Yokoyama, Hiraiwa, Kataoka, Kito, Tsukahara
Fujimoto, Tomiyama, Takashima, Yamada, Yasui, Ijuin, Kadota, Ueki
Takehiko FUJISAWA (2008.4~) Haruo SUZUKI (2004.4~2007.3)
Sei CHOEI (2001.4~2007.2) Katsuya ONUKI (2001.4~2008.2)
Chieko YAMADA (2001.4~2008.3) Hiroyuki NUMAJIRI (2001.8~2007.5)
Naoki OTAKA (2001.9~2008.3) Masaki MIYAKE (2006.8~2007.3)
Asami SAGARA (2006.2~2007.12) Yasuhiro YOKOTA (2002.10~2007.1)
Minehisa SUZUKI (2007. 4~2007. 8)
Experimental Animal DivisionRIKEN BRC Annual Report 2005 ~ 2007
― 43 ―
Mouse strains with high-demand from the community are
maintained as live animals, and others with low-demand
are preserved as frozen embryos. With the release of new
mouse resources and changes in circumstances, we revised
the distribution fees in March, 2008. The number of
registered users is 2,459 (523 for overseas) as of July, 2008.
We have distributed 8,453 (1,475 for overseas) mice to 436
(222 overseas) organizations since 2002. Recipients of our
mice included both academic (88%) and for-profit (12%)
organizations.
(2) Cleanup of mouse strains
The cleanup of mouse strains deposited to RIKEN BRC has
contributed to the high quality standard of animal experiments
in Japan. All the strains are serologically tested for infection
by eight dangerous pathogens. Based on the result of
serological tests, mice are transferred to the Bio-bubble
housing facilities either in negative or positive pressure. Mice
are bred to establish colonies for rederivation by in vitro
fertilization and embryo transfer or Cesarean section. Jcl:ICR
and BALB/cA-nu/+ females are used as recipients of embryo
transfer and as foster nursing mothers, respectively. BALB/
cA-nu/+ mothers have excellent nursing capability to accept
various strains in BRC.
(3) Cryopreservation of mouse embryos and sperm
The cryopreservation of embryos and sperm is a key
technology for the successful operation of the mouse
resource center. Two-cell stage embryos are stored frozen
in a vitrification solution containing ethylene glycol, Ficoll
and sucrose (EFS). The sperm from mutant and genetically
modified strains is also cryopreserved using raffinose and
skim milk as cryoprotective agents. The recovery rate of
each frozen strain is carefully assessed prior to closing live
colonies. The distribution of frozen strains is conducted after
assessing their recovery. By July 2008, 2,123 strains have
been preserved as frozen embryos or sperm.
(4) Quality control
(i) Microbiological monitoring
After cleanup treatments, the mice are monitored by a 2nd
round of microbiological tests for 19 pathogens. If the mice
are free of specific pathogens, they are transferred to the
BRC breeding rooms in the barrier. Periodic microbiological
monitoring of 19 or 22 major pathogens has been done in
every rack of the facility using sentinel mice (70,770 tests
for 3,681 mice from October 2005 to July 2008). The
environment of the facility is quarterly monitored for bacteria
and fungi at 512 points.
(ii) Genetic monitoring
Genetically modified strains such as transgenic and knockout
mice are genotyped using PCR protocols with allele-
specific primers. The genotyping PCR protocols for over
400 strains with high-demand is available on our website as
downloadable pdf files. Genetic backgrounds of congenic
strains are monitored with 7 sets of simple sequence length
polymorphism (SSLP) markers at 75-88 loci in each set.
Inbred and wild-derived strains are also monitored with 15
standard biochemical genetic markers.
(iii) Immunological profiling
The immunological profiling of 43 Inbred and wild-derived
strains is conducted using flow cytometry by the fluorescent
labeling of spleen cell differentiation antigens.
(5) Collection of relevant information and advertisement
In collaboration with the BRC Information Division, our
website is regularly updated and enriched of its content.
Publications by users and relevant literature about the
strains are frequently surveyed by direct email letters and
public databases. A revised list of our strains is submitted
monthly to the IMSR and distributed to the international
scientific community. Periodic e-mail newsletters have been
distributed to over 2,000 users. The e-mail newsletters contain
an article entitled “Mouse of the Month” (Fig.2), topic news
and a report of our recent activities. The mouse resources
and activities of the division are advertised in symposia and
academic meetings.
(6) Training course
The Experimental Animal Division provides training
courses for animal facility managers and laboratory animal
Figure 1. Framework of collection and distribution of mouse resources for advancement of life sciences.
Experimental Animal DivisionRIKEN BRC Annual Report 2005 ~ 2007
― 44 ―
technicians. Each course includes theory and practice of the
mouse facility management and quality control programs such
as microbiological and genetic monitoring tests. We have thus
far accepted 12 trainees from local pharmaceutical and other
companies, the National Laboratory Animal Center of Taiwan,
and the Lanzoh Institute of Biological Products of China.
(7) International collaboration
RIKEN BRC is a founding member of the Federation
of International Mouse Resources (FIMRe). FIMRe is
a collaborating group of mouse repository and resource
centers worldwide, including The Jackson Laboratory and
the European Mouse Mutant Archive (EMMA). We have
participated in IMSR, a one-stop shop of mouse strains
available worldwide. RIKEN BRC promotes collaboration
with Asian countries. We are a founding member of the Asian
Mouse Mutagenesis and Resource Association (AMMRA).
On November 26, 2007, the Bio-Evaluation Center of Korea
Research Institute of Bioscience and Biotechnology and
RlKEN BRC entered into a Memorandum of Understanding to
promote cooperation in areas of mutual interest in laboratory
animal sciences. We started mutual visits with the Laboratory
Animal Center, Institute of Cytology and Genetics, Siberia
Branch of Russian Academy of Sciences in 2008.
(8) Others
Our Division has been designated as a NBRP-rat sub-center
in collaboration with Prof. Serikawa, Kyoto University. Our
mission is to establish a backup storage of NBRP rat strains as
frozen embryos and sperm.
2. Development ProgramOur division develops novel mouse resources and relevant
technologies necessary for the collection, preservation,
quality-control and distribution of the mouse resources. The
following development programs were carried out in the fiscal
years 2006-2008.
(1) Development of novel mouse resources
(i) RIKEN BRC grant for R&D of genetically modified
mouse strains
RIKEN BRC publicly called for applicants in the fiscal years
2007 and 2008 for research and development programs
to generate novel genetically modified mouse strains
of immediate demand by the research community. The
applicants were asked to propose a design of gene constructs
for genetic modifications, and to produce and submit the
recombinant gene constructs to BRC. BRC separately orders
the third parties to make mouse strains after an open bid. The
specialist committee members including those of the BRC
Experimental Animal Steering Committee have selected 12
academic organizations to generate 103 gene constructs for
genetic modifications in 2007, and 11 organizations for 291
genes in 2008.
(ii) Development of genetically uniform mouse strains
Transgenic and knockout mouse strains with high-demand
such as GFP and human disease models were backcrossed
with standard inbred strains to produce genetically uniform
congenic strains.
(iii) Development of Cre-Zoo
Conditional knockout mouse resources have been generated
worldwide for the functional analysis of genes. Tissue-
specific Cre-transgenic mouse strains are essential tools
for dissecting spatiotemporal gene functions together with
conditional knockout mice. In cooperation with the Gene
Engineering Division, we developed several lines of Cre-
transgenic mouse strains driven by tissue-specific promoters.
Figure 2. An excellent mouse model is introduced as “Mouse of the Month” in our e-mail mews.
Experimental Animal DivisionRIKEN BRC Annual Report 2005 ~ 2007
― 45 ―
(2) Development of technologies for quality control and
strain characteristic database
(i) Development of a fine detection method for pathogens
A highly sensitive multiplexed microfluidic immunoassay
system for rapidly detecting pathogenic microbes with a
microvolume of blood sample was developed. Pathogenic
antigens were extracted and purified for the detection system
and positive controls. Genomic materials of 21 pathogenic
microbes were also been prepared as positive controls for
microbiological tests.
(ii) Microsatellite and SNP data
We prepared 7 sets of SSLP markers at 75-88 loci in each set
to examine genetic backgrounds. SNP analysis was carried
out in 153 strains including inbred, congenic, recombinant
inbred and wild-derived strains to clarify their genetic quality
using 15K SNPs of B6-MSM and the Illumina Golden Gate
Mouse SNP panel. These SNP data clearly demonstrated
the genetic relationship of the strains and the subtle genetic
differences among C57BL/6 substrains.
(iii) Development of genotyping methods for genetically
modified strains
We d e v e l o p e d a “ K O s u r v e y ” P C R p r o t o c o l f o r
simultaneously detecting different regions of complex
transgenes, and used it to screen mice in the quarantine and
barrier facilities. Transgenic strains were analyzed with their
genomic sequences flanking transgenes. The information
obtained was used to determine the integration site of
transgenes and to set up genotyping PCR protocols for hemi-
or homo-zygous distinction. Allele-specific PCR protocols
for accurately detecting point mutations and SNPs of the
strains were developed. These technologies and information
have contributed to obtaining accurate information on genetic
modifications and genetic backgrounds.
(iv) RIKEN BRC Mouse Phenome Database (RMPD)
RMPD was developed in collaboration with the BRC
Information Division and made available in our website in
March, 2008. The database includes external measures, blood
pressures, blood cell counts, and blood biochemistry measures
of 140 mouse strains. The data could be displayed as bar
graphs, two-dimensional plots and lists. We also collected
images of the coat colors, histopathological sections and
X-ray images of the skeleton of 104 various inbred and mutant
mouse strains. Behavioral phenotypes were also recorded
using a digital video camera.
(v) Development of novel phenotype data and disease
models
We started collaborations with the Technology and
Development Team for Mouse Phenotype Analysis, Japan
Mouse Clinic and other groups to enrich the phenotype data of
our mice and develop novel human disease models.
(3) Other projects
(i) NBRP genome information upgrading program
The end sequencing of 800,000 cDNA clones from wild-
derived strains, MSM, HMI, PGN2 and KJR was carried
out by collaborating with Prof. Shiroishi of the National
Institute of Genetics and Dr. Abe of the BRC Technology and
Development Team for Mammalian Cellular Dynamics.
(ii) NBRP fundamental technology upgrading program
(2007-2008)
A collaborative program with Prof. Serikawa (Kyoto
University), Prof. Nakagata (Kumamoto University) and
Dr. Ogura (Bioresource Engineering Division) entitled
“Development of transportation system for the mouse and
rat resources” was carried out to develop high-performance
shipping containers for live rodents and their embryos and
sperm.
(iii) Development of Cre-driver strains for neural circuit
genetics
In collaboration with Prof. Tonegawa (MIT), Dr. Itohara
(RIKEN BSI), Dr. Abe (BRC) and Dr. Obata (BRC), we
started a project to generate Cre-driver transgenic mouse
strains for the functional analysis of the neural circuit.
Experimental Animal DivisionRIKEN BRC Annual Report 2005 ~ 2007
― 46 ―
Ugai H., Murata T., Nagamura Y., Ugawa Y., Suzuki
E., Nakata H., Kujime Y., Inamoto S., Hirose M.,
Inabe K., Terashima M., Yamasaki T., Liu B., Nakade
K., Pan J., Kimura M., Saito I., Hamada H., Obata Y.,
Yokoyama K.K.: “A database of recombinant viruses
and recombinant viral vectors available from the RIKEN
DNA bank.” J Gene Med 7, 1148-1157 (2005).*
Toyo-Oka K., Sasaki S., Yano Y., Mori D., Kobayashi
T., Toyoshima YY., Tokuoka SM., Ishii S., Shimizu
T., Muramatsu M., Hiraiwa N., Yoshiki A., Wynshaw-
Boris A., Hirotsune S.: “Recruitment of katanin p60 by
phosphorylated NDEL1, an LIS1 interacting protein,
is essential for mitotic cell division and neuronal
migration.” Hum Mol Genet 14, 3113-3128 (2005).*
Sasaki S., Mori D, Toyo-oka K., Chen A., Garrett-Beal
L., Muramatsu M., Miyagawa S., Hiraiwa N., Yoshiki
A., Wynshaw-Boris A., Hirotsune S.: “Complete loss of
Ndel1 results in neuronal migration defects and early
embryonic lethality.” Mol Cell Biol 25, 7812-7827
(2005).*
Nakamura K., Suzuki Y., Inoue N., Noro C., Suzuki A.:
“Structural characterization of neutral glycosphingolipids
by thin-layer chromatography coupled to matrix-assisted
laser desorption/ionization quadrupole ion trap time-of-
flight MS/MS.” Aral. Chem. 78, 5736-5743 (2006).*
Sato J.J., Tsuru Y., Hirai K., Yamaguchi Y., Mekada
K., ., Takahata N., Moriwaki K.: “Further evidence for
recombination between mouse hemoglobin beta b1 and
b2 genes based on the nucleotide sequences of intron,
UTR, and intergenic spacer regions.” Genes Genet Syst
81, 201-209 (2006).*
Yoshiki A., Moriwaki K.: “Mouse phenome research:
implications of genetic background.” Ilar J 47, 94-102
(2006).*
Yonezawa S., Yoshizaki N., Kageyama T., Takahashi
T., Sano M., Tokita Y., Masaki S., Inaguma Y., Hanai
Publications
1.
2.
3.
4.
5.
6.
7.
【Original Papers】 (*Peer reviewed journals)
A., Sakurai N., Yoshiki A., Kusakabe M., Moriyama A.,
Nakayama A.: “Fates of Cdh23/CDH23 with mutations
affecting the cytoplasmic region.” Hum Mutat 27, 88-97
(2006).*
Kaneko S., Aki I., Tsuda K., Mekada K., Moriwaki K.,
Takahata N., Satta Y.: “Origin and evolution of processed
pseudogenes that stabilize functional Makorin1 mRNAs
in mice, primates and other mammals.” Genetics 172,
2421-2429 (2006).*
Inoue K., Noda S., Ogonuki N., Miki H., Inoue S.,
Katayama K., Mekada K., Miyoshi H., Ogura A.:
“Differential developmental ability of embryos cloned
from tissue-specific stem cells.” Stem Cells 25, 1279-
1285 (2007).*
Ike F., Bourgade F., Ohsawa K., Sato H., Morikawa S.,
Saijo M., Kurane I., Takimoto K., Yamada Y K., Jaubert
J., Berard M., Nakata H., Hiraiwa N., Mekada K.,
Takakura A., Itoh T., Obata Y., Yoshiki A., Montagutelli
X.: “Lymphocytic choriomeningitis infection undetected
by dirty-bedding sentinel monitoring and revealed after
embryo transfer of an inbred strain derived from wild
mice.” Comparative Medicine 57, 272-281 (2007).*
Nakade K., Pan J., Yoshiki A., Ugai H., Kimura M.,
Liu B., Li H., Obata Y., Iwama M., Itohara S., Murata
T., Yokoyama KK.: “JDP2 suppresses adipocyte
differentiation by regulating histone acetylation.” Cell
Death Differ (2007).*
Shinmen A., Honda A., Ohkawa M., Hirose M.,
Ogonuki N., Yuzuriha M., Miki H., Mochida K., Inoue
K., Abe K., Ito M., Ogura A.: “Efficient production of
intersubspecific hybrid mice and embryonic stem cells
by intracytoplasmic sperm injection.” Mol Reprod Dev
(2007).*
Tanaka S., Miura I., Yoshiki A., Kato Y., Yokoyama H.,
Shinogi A., Masuya H., Wakana S., Tamura M., Shiroishi
T.: “Mutations in the helix termination motif of mouse
8.
9.
10.
11.
12.
13.
Experimental Animal DivisionRIKEN BRC Annual Report 2005 ~ 2007
― 47 ―
type I IRS keratin genes impair the assembly of keratin
intermediate filament.” Genomics 90:703-711 (2007).*
Endoh K., Mochida K., Ogonuki N., Ohkawa M. “The
developmental ability of vitrified oocytes from different
mouse strains assessed by parthenogenetic activation
and intracytoplasmic sperm injection.” Journal of
Reproduction and Development 53,1199-1206 (2007).*
Toyo-oka K., Mori D., Yano Y., Shiota M., Iwao H., Goto
H., Inagaki M., Hiraiwa N., Muramatsu M., Wynshaw-
Boris A., Yoshiki A., Hirotsune S.: “Protein phosphatase 4
catalytic subunit regulates Cdk1 activity and microtubule
organization via NDEL1 dephosphorylation.” J Cell Biol
180: 1133-1147 (2008).*
14.
15.
Hirano T., Ike F., Murata T., Obata Y., Uchiyama H.,
Yokoyama K.: “Genes encoded within 8q24 on the
amplicon of a large extrachromosomal element are
selectively repressed during the terminal differentiation
of HL-60 cells.” Mutation Research 640, 97-106 (2008).*
Takimoto K. , Taharaguchi M., Ike F. , Yamada
Y.: “Detect ion of the ant ibody to lymphocytic
choriomeningitis virus in sera of laboratory and newly
isolated strains by ELISA using purified recombinant
nucleoprotein.” Experimental Animals 57, No.4, 357-365
(2008).*
Motokawa M., Harada M., Mekada K., Shrestha KC.:
“Karyotypes of Soriculus nigrescens and Episoriculus
caudatus from Nepal (Soricomorpha, Soricidae).”
Integrative Zoology (in press).*
16.
17.
18.
Oral Presentations
Yoshiki A., Mekada K., Nakata H., Hiraiwa N., Ike F.,
Mochida K., Moriwaki K., Obata Y.: “Establishment of
mouse strain resources in RIKEN BioResource Center.”
International Symposium of the Korean Association for
Laboratory Animal Science, Seoul, Korea, Jun. (2005).
Satoko K., Tsuda K., Mekada K., Moriwaki K., Takahata
N., Satta Y.: “Rapid turnover of regulatory processed
pseudogenes of Makorin1 in rodents and primates.”
Molecular Biology & Evolution 2005, Auckland, New
Zealand, Jun. (2005).
Yoshiki A.: “The RIKEN BioResource Center in the
International Network of Mouse Strain Resources.”
Korean Society for molecular and cellular biology
meeting, Seoul, Korea, Sep. (2005).
Fuchikami T., Mise N., Sugimoto M., Kobayakawa S.,
Kondo M., Ike F., Abe K.: “Dynamics of global gene
expression changes during mouse primordial germ cell
development.” International Symposium on Germ Cells,
Epigenetics, Reprogramming and Embryonic Stem Cells,
Kyoto, Japan, Nov. (2005).
1.
2.
3.
4.
【International Conferences】
Mise N., Fuchikami T., Sugimoto M., Kobayakawa
S., Yuzuriha M., Ike F., Tada T., Ogawa T., Kanaya S.,
Noce T., Abe K.: “Classification of embryo-derived stem
cells and germ cells by genome-wide gene expression
profiling.” International Symposium on Germ Cells,
Epigenetics, Reprogramming and Embryonic Stem Cells,
Kyoto, Japan, Nov. (2005).
Sugimoto M., Mekada K., Karashima Y., Yuzuriha M.,
Ko S. H. M., Nagaraja, Tan S. S., Takagi N., Abe K.:
“Narrowing down the position of the t-complex recessive
lethal mutation tclw5 into 180kb by BAC rescue.” 19th
International Mouse Genome Conference, Strasbourg,
France, Nov. (2005).
Mekada K., Arai F., Murakami A., Oota S., Moriwaki
K., Obata Y., Yoshiki A.: “Analysis of a new recessive
mutant with abnormal walking.” 19th International
Mouse Genome Conference, Strasbourg, France, Nov.
(2005).
Mise N., Fuchikami T., Sugimoto M., Kobayakawa
5.
6.
7.
8.
Experimental Animal DivisionRIKEN BRC Annual Report 2005 ~ 2007
― 48 ―
9.
10.
11.
12.
13.
14.
15.
S., Yuzuriha M., Ike F., Tada T., Ogawa T., Kanaya S.,
Noce T., Abe K.: “Classification of embryo-derived stem
cells and germ cells by genome-wide gene expression
profiling.” 20th IUBMB International Congress of
Biochemistry and Molecular Biology and 11th FAOBMB
Congress, Kyoto, Japan, Jun. (2006).
Doi T., Mise S., Hiraiwa N., Ike F., Yoshiki A., Kasai
K., Obata Y.: “Transcription factor NF-kB is essential at
neonatal stage.” 20th IUBMB International Congress of
Biochemistry and Molecular Biology and 11th FAOBMB
Congress, Kyoto, Japan, Jun. (2006).
Ike F., Yoshiki A.: “Lymphocytic choriomeningitis virus
(LCMV), re-emerging pathogen.” 2nd AALAS Congress
in 2006, Jeju, South Korea, Aug. (2006).
Mise N., Yuzuriha M., Kondo M., Ike F., Araki K., Tada
T., Ogawa T., Kanaya S., Noce T., Abe K.: “Classification
and characterization of ES, EG and primordial germ
cells having different sex chromosome compositions by
microarray-based expression profiling.” 2nd International
Conference on X-Inactivation, Paris, France, Sep. (2006).
Moriwaki K., Ike F., Takakura A.: “Establishment
of detection system for LCMV infection in RIKEN
BioResource Center and CIEA.” US Japan meeting 2006,
Salt Lake City, USA, Oct. (2006).
Fuchikami T., Mise N., Sugimoto M., Kobayakawa
S., Kondo M., Ike F., Abe K.: “Dynamics of global
gene expression in primordial germ cells during mouse
development.” Cold Spring Harbor Laboratory 2006
Meeting on Germ Cells, Cold Spring Harbor, USA, Oct.
(2006).
Nakata H., Kitaura Y., Mekada K., Murakami A., Obata
Y., Yoshiki A.: “The RIKEN BioRessource Center to
disseminate the highest quality mouse resource for
biomedical research.” Super Computing 2006, Florida,
USA, Nov. (2006).
Inoue K, Ogonuki N., Miki H., Noda S., Inoue S.,
Katayama K., Mekada K., Miyoshi H., Ohura A.:
“Differential developmental ability of embryos cloned
from tissue-specific stem cells.” The 33rd Annual
Conference of International Embryos Transfer Society,
Kyoto, Japan, Jan. (2007).
Nakamura K., Suzuki Y., Goto-Inoue N., Yoshida-
Noro C., Suzuki A.: “TLC-MALDI-MS for Neutral
Glycosphingolipids”, Glycobiology and Sphingobiology
2007 (GS2007): Hakomori Commemorative Forum ,
Tokushima, Feb. (2007).
Ike F., Aoki H., Morikawa S., Yoshiki A., Yamagata Y.:
“Identification of mouse antibody reaction by multi-
channel microfluidic immunoassay chip using small
amounts of diluted serum.” FELASA-ICLAS Joint
Meeting 2007, Cernobbio, Italy, Jun. (2007).
Ike F., Kajita A., Aoki H., Kase H., Nagamune T.,
Morikawa S., Obata Y., Yamagata Y.: “Detection of
emerging zoonotic infection in mice by high sensitive
multiplexed microfluidic immunoassay system.” 11th Int.
Conf. on Miniaturized Systems for Chemistry and Life
Sciences (μTAS 2007), Paris, France, Oct. (2007).
Aoki H., Kajita A., Kaneko A., Ishihara M., Hara T.,
Nonaka H., Yamagata Y., Ike F., Kase H.: “Development
of new rapid multiplex microfluidic chip system for
research animal serology monitoring.” 58th AALAS
National Meeting, Charlotte, USA, Oct. (2007).
Ike F., Kajita A., Yoshiki A., Obata Y., Aoki H., Kase
H., Yamagata Y., Morikawa S.: “Serological profiling
of lymphocytic choriomeningitis infected wild-derived
mice.” 58th AALAS National Meeting, Charlotte , USA,
Oct. (2007).
Tanaka S., Miura I., Yoshiki A., Kato Y., Yokoyama H.,
Shinogi A., Masuya H., Wakana S., Tamura M., Shiroishi
T.: “Abnormal Assembly of Keratin Intermediate
Filaments in Mouse Mutations of Type I Inner Root
Sheath Keratin.” 21st International Mammalian Genome
Conference (IMGC2007), (IMGC), Kyoto, Oct.-Nov.
(2007).
16.
17.
18.
19.
20.
21.
Experimental Animal DivisionRIKEN BRC Annual Report 2005 ~ 2007
― 49 ―
22.
23.
24.
25.
26.
27.
28.
Araki K., Takeda N., Yoshiki A., Yamada G., Nakagata N.,
Shiroishi T., Moriwaki K., Yamamura K.: “Establishment
of embryonic stem cell lines derived from Msm/ms
strain originated from mus musculus molossinus.”
21st International Mammalian Genome Conference
(IMGC2007), Kyoto, Oct.-Nov. (2007).
Oota S., Mekada K., Arai F., Obata Y., Fukami K.,
Yoshiki A.: “Four-Dimensional quantitative analysis
on the gait of mutant mice by using the motion capture
technology.” 21st International Mammalian Genome
Conference (IMGC2007), Kyoto, Oct.-Nov. (2007).
Sato J. J., Yamaguchi Y., Ueta J., Suzuki H., Chunyan W.,
Kryukov A. P., Mekada K., Takahata N., Moriwaki K.:
“Genetic characterization of the Wild-derived house mice
in east asia.” 21st International Mammalian Genome
Conference (IMGC2007), Kyoto , Oct.-Nov. (2007).
Miura I., Tanaka S., Yokoyama H., Shinogi A., Kobayashi
K., Kaneda H., Toyoda T., Yoshiki A., Yonekawa H.,
Kikkawa Y., Kunieda T., Tamura M., Masuya H., Wakana
S., Shiroishi T.: “Hair Morphological Mutants Generated
in the RIKEN Mutagenesis Project.” 21st International
Mammalian Genome Conference (IMGC2007), (IMGC),
Kyoto, Oct.-Nov. (2007).
Yokota H., Kakusho N., Mekada K., Murakami A.,
Nakamura S., Ito M., Kase K., Oota S., Fukami K.,
Masuya H., Wakana S., Moriwaki K., Shiroishi T.,
Himeno R., Obata Y., Yoshiki A.: “High-Resolution
full color digital mouse anatomy by three-dimensional
internal structure microscopy.” 21st International
Mammalian Genome Conference (IMGC2007), Kyoto,
Oct.-Nov. (2007).
Kitaura Y., Mekada K., Nakata H., Matsushima Y., Mei-
Ling J., Shiroishi T., Moriwaki K., Obata Y., Yoshiki
A.: “Immunological profiling of laboratory inbred
and wild-derived mouse strains developed in Japan.”
21st International Mammalian Genome Conference
(IMGC2007), Kyoto, Oct.-Nov. (2007).
Yoshiki A., Ike F., Hiraiwa N., Nakata H., Mekada K.,
Kitaura Y., Mochida K., Kadota M., Murakami A., Fujita
M., Ohkawa M., Ogura A., Abe K., Moriwaki K., Obata
Y.: “RIKEN BRC to establish mouse resources of the
highest global standards.” 21st International Mammalian
Genome Conference (IMGC2007), Kyoto, Oct.-Nov.
(2007).
Mekada K., Abe K., Murakami A., Nakamura S., Obata
Y., Yoshiki A.: “Which C57BL/6 substrain is used for
the background strain of your mouse?” 21st International
Mammalian Genome Conference (IMGC2007), Kyoto,
Oct.-Nov. (2007).
Yoshiki A., Ike F., Hiraiwa N., Nakata H., Mekada K.,
Kitaura Y., Mochida K., Kadota M., Murakami A., Fujita
M., Okawa M., Ogura A., Abe K., Moriwaki K., Obata
Y.: “The highest quality mouse resources for global
biomedical researchers from Riken BRC.” Satellite
Meeting. The 10th Annual Meeting of Chinese-Taipei
Society of Laboratory Animal Science. Taipei, Dec.
(2007).
Yoshiki A., Ike F., Hiraiwa N., Nakata H., Mekada K.,
Kitaura Y., Mochida K., Kadota M., Murakami A., Fujita
M., Ogura A., Abe K., Wakana S., Moriwaki K., Obata
Y.: “Distribution of the highest quality mouse resources
from Riken BioResource Center.” The 6th International
Conference on Bioinformatics of Genome Regulation
and Structure, Novosibirsk, Jun. (2008).
Mekada K., Murakami A., Abe K., Moriwaki K., Obata Y.,
Yoshiki A.: “Genetic variation of mouse inbred strain”,
The 6th International Conference on Bioinformatics of
Genome Regulation and Structure, Novosibirsk, Jun.
(2008).
29.
30.
31.
32.
【Domestic Conferences】 Total 68