FACULTAD DE VETERINARIA 2020/21 Year

2020/21 YearFACULTAD DE VETERINARIA

COURSE DESCRIPTION

COURSE DETAILS

APLICACIÓN DE METODOLOGÍAS BIOQUÍMICAS EN VETERINARIATitle (of the course):Code: 101498Degree/Master: Year: 1GRADO DE VETERINARIAName of the module to which it belongs: OPTATIVIDADField: OPTATIVACharacter: OPTATIVA Duration: SECOND TERMECTS Credits: 3.0 Classroom hours: 30Face-to-face classroom percentage: 40.0% Study hours: 45Online platform: moodle

LECTURER INFORMATION

Name: PADILLA PEÑA, CARMEN ALICIA (Coordinador)Department: BIOQUÍMICA Y BIOLOGÍA MOLECULARArea: BIOQUÍMICA Y BIOLOGÍA MOLECULAROffice location: Edificio Severo Ochoa (C6). Primera planta, este.E-Mail: [email protected] Phone: 957218590

Name: REQUEJO AGUILAR, RAQUELDepartment: BIOQUÍMICA Y BIOLOGÍA MOLECULARArea: BIOQUÍMICA Y BIOLOGÍA MOLECULAROffice location: Edificio Severo Ochoa (C6). Primera planta, este.E-Mail: [email protected] Phone: 957218590

PREREQUISITES AND RECOMMENDATIONS

Prerequisites established in the study plan Have the B1 English title.

It is convenient that the student has previously studied Molecular, Animal and Plant Biology, and Physics andChemistry.

Recommendations

INTENDED LEARNING OUTCOMES

To know the physical and chemical bases of biological processes and their applications to veterinarysciences.

CE2

Molecular bases of biological processes.CE3

OBJECTIVES

Understand the fundamentals of basic Biochemistry methodologies, relevant for their multiple applications inVeterinary Medicine. The achievement of these objectives requires a design based on the practical realization inthe laboratory by each student of the experimental blocks specified in the contents.

www.uco.esfacebook.com/universidadcordoba@univcordoba

INFORMACIÓN SOBRE TITULACIONESDE LA UNIVERSIDAD DE CORDOBA

uco.es/grados

APLICACIÓN DE METODOLOGÍAS BIOQUÍMICAS EN PAGE 1 8/ 2020/21 Year


COURSE DESCRIPTION

CONTENT

1. Theory contentsThis subject will provide students with an understanding of the fundamentals and the management of basicbiochemical methodologies relevant to their multiple applications in Veterinary Medicine. The achievement ofthese objectives requires a design based on the practical realization by each student of the experimental blocksthat are specified below:BLOCK I: Isolation and quantification of nucleic acids- Genomic DNA isolation.- Quantification of nucleic acids.BLOCK II: Amplification using the PCR technique, separation and visualization of nucleic acids.- Amplification using the PCR technique.- Separation of nucleic acid by electrophoresis in agarose gel.- Visualization of nucleic acids with gel-red and UV light.BLOCK III: Coupled enzymatic assays for the determination of activities and metabolitesBLOCK IV: Separation, visualization and quantitative analysis of proteins by electrophoresis.BLOCK V: Identification of proteins / antigens by immunochemical assays.- Western blotting.- ELISABLOCK VI: Immobilization of enzymes in alginate.

The subject "Application of Biochemical Methodologies in Veterinary Medicine" will provide students with anunderstanding of the fundamentals and the management of basic biochemical methodologies relevant to theirmultiple applications in Veterinary Medicine. The achievement of these objectives requires a design based on thepractical realization by each student of the experimental blocks that are specified below:BLOCK I: Isolation, quantification, separation and visualization of nucleic acids:- Isolation of genomic DNA from different meat products (hamburger, sausage, kebab ...).- Quantification of nucleic acids.BLOCK II: Amplification of nucleic acids using the PCR technique to identify the animal species from which themeat products used in the previous block come from.- Separation by agarose gel electrophoresis of nucleic acids.- Visualization of nucleic acids with gel-red and UV light.BLOCK III: Enzymatic assays coupled for the determination of activities and metabolites:-Determination of urea in serum and urine of different animals (horse, dog, cow ...).-Determination of glucose in serum from different animals (horse, dog, cow ...).-Determination of alkaline phostatase in animal serum (horse, dog, cow ...).-Determination of creatinine in serum and urine of different animals (horse, dog, cow ...).-Determination of blood hemoglobin (horse, dog, cow ...).BLOCK IV: Separation, visualization and quantitative analysis of proteins by electrophoresis.BLOCK V: Identification of proteins from different species by immunochemical assays:- Enzootic bovine leukosis and / or blue tongue ELISA.BLOCK VI: Construction of a bioreactor: immobilization of invertase in alginate (spherification technique)

2. Practical contents



uco.es/grados



COURSE DESCRIPTION

METHODOLOGY

General clarifications on the methodology (optional)A part of the practical classes will be taught by Daniel Lagal Ruiz.

Methodological adaptations for part-time students and students with disabilities and specialeducational needsThe particular considerations of students who study part-time will be taken into account.

Face-to-face activities

Activity Large group Small group Total

Assessment activities 2 - 2

Case study 1 - 1

Group work (cooperative ) - 1 1

Lab practice - 20 20

Text analysis 3 - 3

Tutorials 2 1 3

Total hours: 8 22 30

Off-site activities

Activity Total

Analysis 20

Information search 5

Reference search 5

Self-study 15

Total hours 45

WORK MATERIALS FOR STUDENTS

Case studiesCoursebookDossierOral presentationsPlacement booklet



uco.es/grados



COURSE DESCRIPTION

EVALUATION

Intendedlearnig C

ase

Stu

dies

Exa

ms

Lab

orat

ory

Pra

ctic

e

Pla

cem

ent

repo

rts

CE2 X X X X

CE3 X X X X

Total (100%)

Minimum grade

10%

4

30%

4

30%

10

30%

4(*)Minimum grade necessary to pass the course

Method of assessment of attendance:

Al ser una asignatura practica, la asistencia es obligatoria.

General clarifications on instruments for evaluation:In each of the instruments selected for evaluation, you must obtain the minimum qualification indicated in thetable.

Clarifications on the methodology for part-time students and students with disabilities andspecial educational needs:During the realization of practical activities will work on the skill: basic management of laboratory instrumentsand preparation of solutions.The particular considerations of the students who study part-time will be taken into account.

Qualifying criteria for obtaining honors:

Según los criterios del articulo 30.3 del Reglamento de Regimen Academico.

BIBLIOGRAPHY

- Devlin, T.M. (2004). Bioquímica, Libro de Texto con Aplicaciones Clínicas. (4ª Ed), Editorial Reverté,Barcelona.- Nelson DL, Cox MM (2018) Lehninger, Principios de Bioqui´mica (7a edicio´n). Ediciones Omega.- Berg JM, Tymoczko JL, Gatto GJ, Stryer L (2019) Biochemistry (9th Ed). MacMillan.- Voet D, Voet JG, Pratt (2016) Fundamentos de Bioqui´mica: La vida a nivel molecular (4a edicio´n). EdictorialMe´dica Panamericana.

1. Basic Bibliography

2. Further reading- Lodish, H, A Berk, ChA Kaiser, M Krieger, H Ploegh, A Amon and MP Scott,. (2013). Molecular Cell Biology,(7th Ed), WH Freeman, New York.- Watson, J.D., TA Baker, SP Bell, A Gann, M Levine y R Losick. (2005). Biología Molecular del Gen. (5ª Ed),Editorial Médica Panamericana.



uco.es/grados



COURSE DESCRIPTION

COORDINATION CRITERIA

Common evaluation criteriaTasks deadlinesTasks performance

SCHEDULE

Period

Ass

essm

ent

acti

viti

es

Cas

e st

udy

Gro

up

wor

k(c

oop

erat

ive

)

Lab

pra

ctic

e

Tex

t an

alys

is

Tu

tori

als

1# Week 0,0 0,0 0,0 3,0 1,0 0,0

2# Week 0,0 0,0 0,0 3,0 1,0 0,0

3# Week 0,0 1,0 1,0 3,0 0,0 0,0

4# Week 0,0 0,0 0,0 4,0 0,0 0,0

5# Week 0,0 0,0 0,0 4,0 0,0 0,0

6# Week 0,0 0,0 0,0 3,0 1,0 0,0

8# Week 2,0 0,0 0,0 0,0 0,0 3,0

Total hours: 2,0 1,0 1,0 20,0 3,0 3,0

The methodological strategies and the evaluation system contemplated in this Course Description will be adapted according tothe needs presented by students with disabilities and special educational needs in the cases that are required.

CONTINGENCY PLAN: CASE SCENARIO A

Case scenario A will correspond to a diminished on-site academic activity due to social distancingmeasures affecting the permitted capacity of classrooms.

General clarifications on the methodology on case scenario AA multimodal (hybrid) teaching system will be adopted, combining both on-site and remote classes viavideoconference (synchronous) that will be held in the timetable approved by the corresponding Faculty or School.The time distribution of teaching activities (both on-site and remote) will be decided by the aforementionedFaculties and Schools bearing in mind the permitted capacity of classrooms and social distancing measures asestablished at that time.Se incorporaran actividades asíncronas de refuerzo basadas en vídeos tutoriales y material docentecomplementario.A part of the practical classes will be taught by Daniel Lagal Ruiz.

METHODOLOGY



uco.es/grados



COURSE DESCRIPTION

EVALUATION

Intendedlearnig C

ase

Stu

dies

Exa

ms

Lab

orat

ory

Pra

ctic

e

Pla

cem

ent

repo

rts

CE2 X X X X

CE3 X X X X

Total (100%)

Minimum grade

10%

4

30%

4

30%

4

30%


Al ser una asignatura practica, la asistencia, bien sea presencial o virtual, es obligatoria.

Method of assessment of attendance (Scenario A):

In each of the instruments selected for evaluation, you must obtain the minimum qualification indicated in thetable.The evaluation of the case studies, placements reports and the laboratory practices are part of the continuousevaluation. There will also be an exam, as indicated in the table.In the extraordinary call for the course 20-21, all the continuous assessment instruments (case studies,placements reports and the laboratory practices) that were passed in the previous course will be added, and thosethat are pending will be included in the extraordinary call together with the final exam. In this case, they wouldhave to prepare a case studie, a placement report and answer some questions about laboratory practices, if theyhad not passed it in the previous course.

General clarifications on instruments for evaluation (Scenario A):

Clarifications on the methodology for part-time students and students with disabilities andspecial educational needs (Scenario A):During the realization of practical activities will work on the skill: basic management of laboratory instrumentsand preparation of solutions.The particular considerations of the students who study part-time will be taken into account.

Qualifying criteria for obtaining honors (Scenario A):

SegÃºn los criterios del articulo 30.3 del Reglamento de Regimen Academico.

CONTINGENCY PLAN: CASE SCENARIO B

Case scenario B will bring about a suspension of all on-site academic activities as a consequence ofheath measures.



uco.es/grados



COURSE DESCRIPTION

General clarifications on the methodology on case scenario BOn-site teaching activities will be held via videoconference (synchronous) in the timetable approved by thecorresponding Faculty or School. Alternative activities will be proposed for reduced groups in order to guaranteethe acquisition of course competences.Se incorporaran actividades asíncronas de refuerzo basadas en vídeos tutoriales y material docentecomplementario.A part of the practical classes will be taught by Daniel Lagal Ruiz.

METHODOLOGY

EVALUATION

Intendedlearnig C

ase

Stu

dies

Exa

ms

Lab

orat

ory

Pra

ctic

e

Pla

cem

ent

repo

rts

CE2 X X X X

CE3 X X X X

Total (100%)

Minimum grade

10%

4

30%

4

30%

4

30%


Al ser una asignatura practica, la asistencia, bien sea presencial o virtual, es obligatoria.

Method of assessment of attendance (Scenario B):

General clarifications on instruments for evaluation (Scenario B):In each of the instruments selected for evaluation, you must obtain the minimum qualification indicated in thetable.

Moodle Tools

Est

ud

io d

e ca

sos

Exá

men

es

Info

rmes

/mem

oria

sde

prá

ctic

as

Prá

ctic

as d

ela

bora

tori

o

Asistencia X X X

Cuestionario X X X

Pruebas simultáneas porvideoconferencia

X

Tarea X X

Videoconferencia X



uco.es/grados



COURSE DESCRIPTIONThe evaluation of the case studies, placements reports and the laboratory practices are part of the continuousevaluation. There will also be an exam, as indicated in the table.In the extraordinary call for the course 20-21, all the continuous assessment instruments (case studies,placements reports and the laboratory practices) that were passed in the previous course will be added, and thosethat are pending will be included in the extraordinary call together with the final exam. In this case, they wouldhave to prepare a case studie, a placement report and answer some questions about laboratory practices, if theyhad not passed it in the previous course.

During the realization of practical activities will work on the skill: basic management of laboratory instrumentsand preparation of solutions.The particular considerations of the students who study part-time will be taken into account.

Clarifications on the methodology for part-time students and students with disabilities andspecial educational needs (Scenario B):

Qualifying criteria for obtaining honors (Scenario B):

SegÃºn los criterios del articulo 30.3 del Reglamento de Regimen Academico.



uco.es/grados


Documents

FACULTAD DE VETERINARIA 2020/21 Year