Environmental and Bio-safety Review of Animal Health Laboratory Network in

Bangladesh

Introduction:

The Avian Influenza Preparedness and response Project (AIPRP, 2007-12) aims to minimize the

threat posed by HPAI infection and other zoonoses by assisting the Government of Bangladesh

prepare for, control and respond to avian influenza pandemics and related emergencies.

FDIL Feni FDIL Jessore

FDIL Barisal

FDIL Sylhet

FDIL Joypurhat

FDIL Gaibanda

FDIL Sirajganj

CDIL

BLRI FDIL Manikganj

FDIL Feni

FDIL Barisal

FDIL

Jessore

FDIL Manikganj

BLRI

CDIL

FDIL Sylhet

Gaibanda

FDIL Sirajganj

Joypurhat

FDILs-CDIL-BLRI location in Bangladesh

The Department of Livestock Services (DLS) within the Ministry of Fisheries & Livestock

(MOFL) has one Central Disease Investigation Laboratory (CDIL) and network of 7 Field

Disease Investigation Laboratories (FDILs) for livestock disease diagnosis. The 7 FDILs

(Manikganj, Feni, Sirajganj, Joypurhat, Gaibanda, Barisal and Sylhet), CDIL and Bangladesh

Livestock Research Institute (BLRI), the National Reference Laboratory (NRL) for Avian

Influenza (AI), all the animal health laboratories are supported by AIPRP through WB funding

specifically refurbished and equipments in order to strengthen Animal Disease Surveillance &

Diagnostic Capacity particularly by improving laboratory diagnosis capacity. Construction of a

new FDIL located at Jessore district is in process. Thus AIPRP laboratory up gradation and

activities require complying with the existing environmental regulations in Bangladesh such as

Environment Conservation Rules (ECR‘1997 amended in 2000) and especially with

environmental category (Red, Orange- A, Orange-B and Green) of the Department of

Environment (DoE) of the Government of Bangladesh (GOB). Also comply with Biosafety

Guideline of GOB (2005), since it involves moderate environmental impacts such as inadvertent

spread of the AI virus during culling, transport and disposal of carcasses, animal waste and litter

mainly from the life bird market, commercial farms and potential backyard poultry sector

including biological and chemical wastes from the animal health laboratories.

The AIPRP is assessed and assigned as World Bank category B (partially assessed).

Safeguard Policies Triggered by the project Yes No

Environmental Assessment (OP/BP 4.01) X

Natural Habitats (OP/BP 4.04) X

Pest Management (OP 4.09) X

Cultural Property (OPN 11.03, revised as OP 4.11) X

Involuntary Resettlement (OP/BP 4.12) X

Indigenous Peoples (OP/BP 4.10) X

Forests (OP/BP 4.36) X

Safety of Dams X

Projects in Disputed Areas (OP/BP 7.60) X

Projects on International Waterways (OP/BP 7.50) X

With the following Terms of Reference (below), a Laboratory Biosafety Assessment Expert is

recruited and deployed in February 2011 for three months under Food and Agricultural

Organization (FAO) of the United Nations –Technical Assistance Service (TAS) agreement.

Terms of Reference (ToR):

Review and assess environmental conformity in newly developed or refurnished

Government of Bangladesh (GOB) animal health laboratories, identified as Field Disease

Investigation Laboratories (FDIL), which were supported by the AIPRP through funding

of the World Bank. Specifically , the refurbished laboratories include Bangladesh

Livestock Research Institute (BLRI) in Savar, Central Disease Investigation Laboratory

(CDIL) in Dhaka, and the seven Field Disease Investigation Laboratories (FDILS)

located at Manikganj, Feni, Sirajganj, Joypurhat, Gaibandha, Barisal and Sylhet and the

soon to be newly constructed FDIL located at Jessore;

Liaise with program technical staff as well as with laboratories technical staff and

stakeholders to understand program setting and to identify biosafety concerns related to

laboratories operation;

Work closely with the AIPRP Monitoring and Evaluation (M& E) Experts on identifying

key indicators that measure biosafety performance of the laboratories for inclusion in the

AIPRP M&E System;

Report on observations related to biosafety concerns to the TAS-Team Leader and the

AIPRP Project Director as well;

Assess current capacity of laboratory technical staff in addressing environmental

concerns that are related to laboratory operations;

2. Methodology:

As part of the Terms Of Reference (TOR) and to comply the mission enquiries of the World

Bank, a work plan was prepared and endorsed by the team leader, Technical Assistance Service

(FAO-TAS) and AIPRP Project Director. The AIPRP Project Director also issued an Official

Letter for visiting the Field Disease Investigation Laboratories (FDILs), Central Disease

Investigation Laboratory (CDIL) and Bangladesh Livestock Research Institute (BLRI).

Following the guidelines of international laboratory standards, a Laboratory Safety Inspection

Checklist consists of 35 questions and following the guidelines of Environmental and Social

Safeguard Management Framework (ESMF) prepared by the Social Development Foundation

(SDF) of Bangladesh and the guidelines of Department of Environment (DoE), an

Environmental Checklist consists of 11 questions along with issues related to Environment

Management Plan (EMP) were prepared and administered for 7 ( Manikganj, Joypurhat,

Gaibanda, Sirajganj, Feni, Sylhet, Barisal) Field Disease Investigation Laboratories (FDILs)

including Jessore proposed FDIL site. Central Disease Investigation Laboratory (CDIL) and

Bangladesh Livestock Research Institute (BLRI) were also visited. Photographs were taken from

the FDILs, CDIL and BLRI for documentation. The expert collected the full address of the focal

person and communicated to the FDIL- in Charge i.e. the Principal Scientific Officers (PSOs) /

Senior Scientific Officer (SSO) of the concerned laboratories well ahead through cell phone.

Using the checklists, the FDIL- in charges was interviewed accompanied by the laboratory

technical staffs.

3. BIOSAFETY AND ENVIRONMENTAL CONCERNS OF THE LABORATORIES:

The expert developed a checklist on Laboratory Safety Inspection Checklist (Annex:1) that

covers compliance with safety measures for labs. The main themes covered by the checklist

includes signs used in labs (boards, smoking, hazards etc.), general condition of the labs,

building status, safety measures, solid waste disposal, fire hazards, Personal Protective

Equipment (PPE), and bacterial contamination control.

Development of Environmental Checklist (Annex:2). Issues covered by this checklist include

water quality (surface and ground), chemicals and infectious agents released from the labs,

pollution, biodiversity destruction, noise level, and public health hazard. The checklist was used

to partially assess compliance with environmental category (Red, Orange- A, Orange-B and

Green) of the Department of Environment of GOB.

Nine labs were visited by the expert, including seven FDILs, BLRI and CDIL. The above

mentioned checklists were used to assess compliance with the measures identified in the

assessment (FDIL-Manikganj, FDIL- Joypurhat, FDIL-Gaibanda, FDIL-Sirajganj, FDIL-Feni

and FDIL-Sylhet, FDIL-Barisal, CDIL and BLRI). The visits took place during the period March

22 - April 9, 2011. Photographs of the laboratories, especially newly renovated rooms,

passageways, storerooms, work areas and aisles were taken.

Interview the FDIL /CDIL/BLRI. The Principal Scientific Officers (SPO) and Senior Scientific

Officers (SSO) in-charge of the laboratories were interviewed by the expert during the visits. The

checklist questionnaires were administered in consultation with the laboratory officer-in-charge

and the laboratory technical staffs.

OBSERVATION, FINDINGS AND RECOMMENDATIONS

General Signs and Information: General signs and information of FDILs, CDIL and BLRI

are posted while No Smoking signs are missing. It is recommended to post the No Smoking

signs and information of the laboratories. It is also noted that the word “Animal” is missing

from FDIL so the FDIL can be renamed as Animal Disease Diagnosis Laboratory (ADDL).

Biohazard Communication and Universal Biohazard Sign of the laboratories: Biohazard

should be communicated through labeling and biohazard signs, where biologically active

substances and wastes are used, handled or stored. Laboratories should use the universal

biohazard symbol and is required for bags, sharps containers, refrigerators and freezers used

to store, transport or ship biologically active substances or any hazardous microorganisms. In

addition to labels, to post a biohazard sign at the entrance to a laboratory is utmost essential.

The sign should include the universal biohazard symbol, the agent in use, the criteria for

entry and the Biosafety Level. Labs also require the name and telephone number of a contact

person but biohazard signs and information is missing at all laboratories except for BLRI and

CDIL. Although general signs with Red Cross mark is posted to the entrance door of the

newly renovated buildings of all the FDILs. There is a need to post the Universal Biohazard

signs and information.

Construction: The World Bank funded AIPRP construction and renovation works of all

FDILs, CDIL and BLRI have been completed and the laboratory rooms are ready except for

FDIL-Sirajganj and FDIL-Feni. The refurnished works at FDIL-Sirajganj is supposed to be

completed within one month of the visit (24 March 2011). Due to the construction works,

laboratory equipments, chemicals, microscope, official paper and records were dumped together

especially at FDIL-Sirajganj and FDIL-Feni that may hamper the laboratory analysis in future.

Also to verify the effectiveness of the chemicals and reject old chemicals.

The expert visited the FDIL-Jessore site to be constructed sooner and communicated the

environmental and biosafety concerns with Deputy Director, in charge of Divisional Livestock

Office,Khulna located at Jessore district. Thus environmental and biosafety issues will be given

priority while construction for example setting treatment plant for waste water discharged from

the laboratory with compliance to DoE standards. In order to design, construct and operate a

new FDIL, the expert also shared the following; that the laboratory shall have procedures for

monitoring, controlling and recording environmental conditions where applicable, such as:

• acceptable lighting, • replenishment of consumables used in reagent water and/or dilution water

treatment, • water quality characteristics as required, especially conductivity on a daily or as used

basis and corrective actions taken for non-conformance, • temperature, • humidity, • storage

temperatures and corrective actions taken for non-conformance and the director should bear in

mind, consult with engineer for environment friendly FDIL setting at Jessore.

Installation of AIPRP machineries and equipments: One of the major observations is

about the installation of the AIPRP equipments and machineries those reached by international

bidding by the contractors at different FDILs such as FDIL-Joypurhat, FDIL- Gaibandha, and

FDIL-Sirajganj, FDIL-Barisal. There is a risk of the equipments and its appropriate function if

not properly installed soon. It is highly recommended to set the equipments sooner by involving

experienced and skilled technicians. The AIPRP laboratory machineries and equipments such as

incinerator, generator has not reached to FDIL-Manikganj yet. Absence of incinerator and

generator increases the chances of decontamination process of laboratory wastes.

Shortage of laboratory staff: During the visit, it was found that the most laboratory staff in

the FDILs is on deputation whom in turn laboratory works suffers and delayed a lot. The Senior

Scientific Officer (SSO) and two Scientific Officer (SO) out of three were on deputation at

FDIL-Barisal. Laboratory works also suffers from the deployment of field extension personnel

at laboratories. There is a need to develop laboratory specialists and be posted within the lab

position only.

Biosafety and Biosafety Procedures: Biosafety or biological safety is the policies and

procedures adopted to ensure the environmentally safe application of biotechnology. Biosafety

is concerned with; a) Physical (building), b) Chemical and c) Biosafety (organism).

Avian Influenza (AI) is a highly contagious disease of birds and, although it shares

similarities with human influenza, the viruses responsible for AI can not be transmitted easily to

human. However, AI viruses have been transmitted to human sporadically and under specific

conditions that have included:

Direct contact with sick or dead birds

Contact with surfaces that have been contaminated with the excreta (faeces) and /or

secretions of infected birds

Contact of oral , ocular or nasal mucosa with infected aerosols

Consumption of uncooked meat or blood of infected birds

Accordingly, the issues of biosafety amongst personnel who are in contact with the virus

is of the utmost importance. Personnel at risk of exposure to the virus fall into two

categories. The first group consists of those who are involved in the control of outbreaks

and the eradication of AI and whose tasks include the culling of infected birds, carcass

elimination and the cleaning and disinfection of premises. The second group at risk of

exposure includes laboratory-based personnel who are involved in the reception and

processing of contaminated specimens and samples that contain the virus and there is a

great need for biosafety precautions for both field –based (group-1) and laboratory-based

personnel (group-2).

Group 2 or Laboratory-Based Personnel

Laboratory safety is the responsibility of all supervisors and laboratory

employees. Individual workers are responsible for their own safety and that of

their colleagues.

Good laboratory practice is fundamental to laboratory safety and virus

depending on the risk associated with the infectious material being

manipulated.

Animal clinical specimens from suspect highly pathogenic avian influenza (HPAI)

cases may be tested by polymerase chain reaction (PCR) assays using standard

biosafety level 2 (BSL 2) work practices in a Class II biological safety cabinet.

Commercial antigen detection testing can also be conducted underBSL 2 levels to

test for influenza. In addition, specimens may be processed for packaging and

distribution to diagnostic laboratories for further testing in a BSL 2 laboratory.

HPAI must be worked with under biosfety level 3+ laboratory conditions.

Therefore, manipulations involving growth of the agent should be in a BSL 3+

laboratory using BSL 3+ operational practices. These include controlled –access

double-door entry with changing room and shower, use of respirators,

decontamination of all wastes and showering of all personnel.

When laboratories do not meet BSL 2 containment conditions, specimens from

suspected or confirmed HPAI cases should be sent to suitably equipped reference

laboratories for further processing.

Thus it can be stated that neither the FDIL nor CDIL is capable of handling HPAI

viruses so it is strongly recommended to use the BLRI 2+ facility at present. Also

keep liaison with ICDDR,B; first BSL 3 in Bangladesh , can determine the

prevalence of Mycobacterium tuberculosis , HIV and influenza virus and can

assist the Government of Bangladesh with research on deadly diseases and

provide an accurate diagnosis using the equipment installed in the laboratory.

There is also a great need to upgrade the CDIL gradually because CDIL

represents DLS and is responsible for testing all kind of animal ( crocodile of

Dhaka Zoo, crow of Dhaka city) health diseases and general public visits with

their animals regularly.

Laboratory facilities: The laboratory is to be separated from areas which are open to

unrestricted traffic flow within the building. Passage through two sets of doors is the basic

requirement for entry into the laboratory from access corridor or other contiguous areas. Physical

separation of the high containment laboratory from access corridor or other laboratories or

activities may also be provided by a double-door cloth change room (showers may be included),

air lock, or other access facility which requires passage through two sets of doors before entering

the laboratory (Biosafety Guidelines of Bangladesh, 2005) but during the visit, it is noticed that

FDIL-Manikganj corridor is partially blocked by vehicles (lorry, truck, microbus, taxi cab)

because of the Manikganj District bus terminal. Vehicles mostly disorders vehicles are placed at

terminal compound and the FDIL-Manikganj corridor are located attached to bus terminal. Both

noise and air pollution from the Dhaka- Aricha /Paturia highway transports is a concern for not

only the laboratory but also the working environment of the District and Upazila Livestock

Office located within the same campus of FDIL-Manikganj. Signboard of FDIL with precaution

messages is required to be posted prominently and there is a great need of issuing a letter from

high official of DLS for shifting the bus terminal to nearby location.

Walls, floors and ceilings of the laboratories are constructed to form a sealed internal shell that

facilitates fumigation and is animal and insect proof. The internal surface of this shell are

resistant to liquids and chemicals. Thus facilitating cleaning and decontamination of the area. All

penetrations in these structures and surfaces are sealed. Any drains in the floors should contain

traps filled with a chemical disinfectant of demonstrated efficacy against the target agent and

these are connected directly to the liquid waste decontamination system. But FDIL-Feni

building is very old and do not comply the requirement of Biosafety Guidelines of Bangladesh as

the laboratory is concern. Following the instructions of higher authority of DLS, the FDIL-

Barisal has formed a four member biosafety team on 13 December 2010.

The laboratories should comply with the Biosafety Guidelines of Bangladesh, 2005

A biosafety manual should be prepared specifically for the FDIL/CDIL laboratories.

Personal Protective Equipment (PPE): Personnel who are at risk of infection or who are in

direct contact with potentially infected birds must wear the PPE.

Protective, disposable, long-sleeved overalls and an impermeable apron

Disposable head covers/bouffant caps

Rubber or polyurethane boots that can be washed and disinfected or disposable shoe-

covers.

Rubber gloves that can be washed and disinfected

Disposable protective face mask

Protective visor to protect against splashes from contaminated material

Protective goggles

After use, the PPE should be removed and the hands washed and disinfected in the

following order:

1. Gloves

2. Overalls

3. Washing and disinfection of hands

4. Protective googles

5. Visor and face mask

6. Washing and disinfection of hands

The above mentioned protection and procedures are instructed to follow for PPE measures for

the laboratories but are not strictly followed for the animal health laboratories (FDIL/CDIL) in

Bangladesh. The laboratories especially BLRI and CDIL are concerned about PPE and follow

the procedures especially after AI outbreak but are recommended improving and practicing the

PPE procedures regularly.

Standard Operating Procedures (SOP): A standard laboratory should have written

Standard Operating Procedures (SOP) approved by the management and be available for;

A) Receipt, identification, labeling, handling, sampling and storage

B) Use maintenance, cleaning, calibration of measuring apparatus and environment control

equipment, preparation of reagents.

C) Record keeping, reporting, storage and retrieval, coding of samples, data analysis,

preparation of reports, indexing system, handling of data including the use of computerized

data system that are intended to ensure the quality and integrity of laboratory operation.

Published text book, journals for example; FAO that publish books in the field of disease

outbreaks for example Biosafety Procedures. Ref. William G. Dundon, Capua, D. J.

Alexander (eds.) Avian Influenza and Newcastle disease@ Springer-Verlag Italia 2009 may

be used as supplements to these SOP.

The SOP is available in the FDILs for different tests such as media, culture, and blood but not

well documented and printed for AI disease. The CDIL follow the Manual of Veterinary

Investigation (Laboratory Techniques). It is strongly recommended to prepare and print SOP for

AI for all FDILs. Training on Standard Operating Procedures (SOP) for laboratory technician

and officers is required for enhancing knowledge on environment and biosafety. There is a need

to develop laboratory manual on environment and biosafety.

Pit: Following the AI outbreak in poultry and launching of AIPRP in 2007, Pit has been

constructed at all FDILs for the decomposition of dead birds especially the samples/carcasses

and other wastes from the laboratories are dumped at pit for biological degradation. But odour

from improper burial pit construction and operation at FDIL-Manikganj and FDIL-Joypurhat,

and FDIL-Feni are creating public health hazards. Also hampers the working environment of

District, UPZ livestock office including laboratory officials working in the same campus. There

is a need to accelerate the decomposition process. Pit construction of FDIL-Sirajganj is located

adjacent to the laboratory and lies at soil surface level so there is a risk of environmental

contamination during rainfall and flooding.

Mitigation measures:

Excavation of a burial pit

Placing carcasses within deep burial pit

Covering carcasses and other wastes with soil (about 40 cm) to

i) prevent carcasses from rising out of the pit,

ii) prevent scavengers digging up carcasses

iii) help filter out odours and

iv) absorb the fluids of decomposition

Adding an unbroken layer of slaked lime [Ca (OH)2] to protect carcasses from

being uncovered by carnivores and eathworms

Fencing of the burial pit

Use PPE to ensure hygiene and safety of personnel working at pit site

Laboratory Wastes: Usually chemical and biological wastes (sharp materials such as

needles, syringe, slide, pipettes, cotton, gauge, plastics) generated from the laboratory are placed

in a basket first and carried or thrown to pit mostly in the weekend. However it is important to

decontaminate the matrix using autoclave, incinerator and sterilizer but incinerator is yet to

install in most laboratories.

Hospital treatment: There may be a risk of spreading zoonotic viruses from hospital

treatment (decontamination) of the District and UPZ Livestock offices that are located within the

same campus of the laboratory and visited by a large number of cattle, goat, pet dog, and cat and

life birds everyday. Safe distance and barrier can be maintained between the Dist./UPZ livestock

office and the laboratory.

Fire Extinguisher: Fire extinguishers ranges (1-3) are available at all the laboratories

but, the efficiency of the fire extinguisher is a question to all if it works during fire thus fire

extinguishers requires verifying the presence of gases or empty. Again there is a need for

demonstration of the uses of fire extinguishers for the laboratory staff in order to protect fire

hazard thus save life and resources from danger.

Municipal dust-bean: Municipal dust-bean of Joypurhat is now located within the

office compound of FDIL-Joypurhat campus of Joypurhat municipality. Thereby the wastes from

the surrounding households are dumped into the municipal bean creating public health hazard.

Eventually the bean may contaminate the ground water and FDIL may be alleged. Besides

working environment of the laboratory staff is disturbed and public health hazard It is

recommended to shift the municipal bean to outside FDIL compound.

Wastewater: Wastewater discharged from the laboratories is not tested as per the

guidelines of Department of Environment (DoE) thus required regular monitoring avoiding

hazardous risks. There is a need for Environmental Clearance Certificate from DoE for the

laboratories under AIPRP that consists of 7 FDILs, CDIL and BLRI where both bacterial and

chemical contamination may be a concern.

Gas cylinders: Compressed gas cylinders should be properly secured and labeled for

safety purposes. Safety guidelines must be followed for placing the cylinder in lab room. But it is

noticed that the gas cylinders placed with chemicals at FDIL-Gaibanda and there is need to

undertake precautionary measures avoiding fire hazard.

Environmental Impacts and Mitigation

Decontamination:

Improper decontamination procedure may expose laboratory staff to the decontaminant agents.

These can be summarized as follows;

Spread of disease as a result of improper/ inadequate decontamination

Health risk (intoxication) for the laboratory staff carrying out the disinfection

Health risk for the neighboring people from improper decontamination procedure of the

pit

Contamination of soil and water by the decontamination agents

Damage to natural flora and fauna caused by the decontaminant agent

Cross –contamination, Spread of Infections and Waste Disposal from Laboratories

Unsafe procedures in laboratories especially unhygienic condition of FDIL- Feni

postmortem room and pit can cause cross-contamination and /or spread of infections

among the staff and may pose environmental and public health risks. Specifically, the

following risks are associated with the improper disposal of wastes from the

laboratories:

Health risk for the laboratory staff and people coming in contact with them

Health risk for the personnel handling the wastes

Contamination of soil and water

Health risk for the nearby population

Mitigation:

Implementation of biosafety level standards in the laboratories

Implementation of standard safety procedures and use of PPEs

Development and implementation of the procedures and guidelines for

segregation, handling and disposal of laboratory wastes

Appropriate design of disposal wells

Provision and use of PPEs during the waste handling and disposal

Training of laboratory technicians on biosafety level standards and improve

the effectiveness and safety over existing avian influenza handling and

testing procedures, use of PPEs, environmental aspects and mitigation

measures

Construction Activities:

Construction activities may contaminate air and water.

Air pollution caused by the exhaust from the vehicles and construction equipment.

Inappropriate disposal of construction waste may cause soil and water contamination.

as well as public nuisance.

Noise from the construction activities may be a source of nuisance to the nearby

people

Safety hazard for the construction workers

Mitigation:

In order to mitigate the environmental impacts that may be caused by the construction

activities, the following mitigation and control measures will be enforced:

The standard good construction practices will be implemented.

- The construction machinery and vehicles will be kept in good working condition,

avoiding excessive exhaust emissions

- Waste effluents (such as used oils) and contaminated water will not be released to the

environment

- Excess construction material and scrap will not be left at the laboratory site

- Noise emissions will be minimized using silencers and sound barriers

Environmental training to the construction staff on environmental practices, waste

disposal, safety aspects and public nuisance

An Environmental and Social Safeguard Management Framework (ESSMF) and an

Environmental Management Plan (EMP) that outlines the measures proposed to address all

potential adverse environmental and social impacts, has been prepared thus serve as a guide to

ensure that the project don’t causes any potential indirect and/or long-term impacts.

It is highly recommended to follow the EMP especially during culling, transport and disposal of

carcasses, animal waste, and chemicals from the laboratories.

ANNEX: 1

Department of Livestock Services, Ministry of Livestock and Fisheries, GOB

LABORATORY SAFETY INSPECTION CHECKLIST

Bangladesh Livestock Research Institute (BLRI)/ Central Disease Incidence Laboratory (CDIL)/

Field Disease Incidence Laboratories (FDILs)- Manikganj/Feni/Sylhet/

Joypurhat/Gaibandha/Sirajganj/Barisal/Jessore

Date:---------------------------- Name of FDIL:--------------------------------------------

Inspected by:--------------------------------- Principle Investigator:---------------------------------------

---

Questions Ye

s

No N

A

1. General signs and information properly posted?

2. No smoking signs prominently posted?

3. All floors clean and dry?

4. Passageways, storerooms, work areas and aisles clear?

5. Proper illumination provided in the actual work area?

6. Proper clothing being worn by employees in the work area?

7. Work areas have at least two exits available (if necessary)?

8. Exits are visible and unobstructed?

9. Are standard operating procedures (SOP) readily available to

workers?

10. Are emergency telephone numbers posted?

11. Are telephones in the work areas conveniently located?

12. First-aid supplies adequate for potential hazards& available?

13. Safety shower visible, operational & easily accessible?

14. Eyewash visible, operational & easily accessible?

15. Hand wash station available and easily accessible?

16. Is the chemical spill kit visible and easily accessible?

17. Access to electrical panels is unobstructed?

18. Are all electrical switches marked to show their purpose?

19. Electrical and or phone cables properly secured on the floor?

20. Chemicals stored by classification not alphabetically?

21. All chemicals are properly labeled

22. Do employees follow good housekeeping practices

23. All materials are pilled racked or stored in a safe manner?

24. Step stools available to reach high places in the work area?

25. Are the work areas or bench tops uncluttered?

26. Are fume hoods certified and properly operating?

27. Flammable storage cabinets available & in good condition?

28. Fume hoods & biosafety cabinets uncluttered & accessible?

29. Waste containers properly labeled and in good condition?

30. Sharp containers available and in good condition?

31. Refrigerated storage is monitored for temperature control?

32. Compressed gas cylinders properly secured and labeled?

33. Portable fire extinguishers provided in adequate number &

type?

34. Are fire extinguishers mounted in accessible locations?

35. Explicit instructions posted for acid splashes & acid spills?

ANNEX:2

Environmental Checklist (Please tick mark in the right place)

SITE: (FDIL-Manikganj, FDIL- Feni, FDIL- Sirajganj, FDIL- Joypurhat, FDIL- Gaibandha,

FDIL-Barisal, FDIL-Sylhet, FDIL-Jessore) / CDIL- Dhaka /BLRI- Savar/:

1. Does the scheme‘s implementation interrupt the natural flow of river, canal or

any stream?

.

2. Does the scheme‘s/LAB implementation increase the possibility of groundwater

pollution?

.

3. Does the scheme‘s/LAB implementation increase the possibility of surface water

pollution?

.

4. Does the scheme/LAB generate any waste?

.

5. Is there any waste management plan for the scheme/LAB if it generates waste?

(Please include the waste management plan)

.

6. Is there any chance of increases public health hazard from throwing waste into

open water bodies?

.

7. For constructing of irrigation canal, is there any chance to create problem to

water bodies that are used for irrigation?

.

8. For sanitary latrine construction, is there any drinking water source near 30 ft

distance of the latrine?

.

9. Is there any chance of tree cutting during the implementation of the scheme? (If yes

then please specify the number of trees involved)

.

10. Is there any chance of destruction of biodiversity (fish, birds and animals)

habitat by the implementation of the scheme?

.

11. Mitigation measures in place

ANNEX: 3

Photographs of FDILs, CDIL and BLRI