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Fast Track Solutions for Every Lab
� www.phenomenex.com
PL
dp
Ndp
Efficiency Equation
N = Efficiency
L = Column Length
dp = Particle Size
h = Reduced Plate Height
kNk
Rs
Pressure Equation
P = Pressure
η = Viscosity
φ = Packing Impediment
µ = Linear Velocity
L = Column Length
dp = Particle Size
Imagine a speeding train—with no tracks to control it.
Sound ridiculous? It is. A fast train needs to have direction, be on schedule, and get you where you need to go.
The Truth about Fast LC
Optimizing Performance
Selectivity of the column stationary phase is the single largest factor in the resolution equation.
• Maximum resolution enables scientists to obtain more information, produce superior results, and decrease run times of the most demanding separations.
• Phenomenex offers a broad range of high quality bonded phases on ultra-pure silica and TWIN™ Technology based media to maximize resolution.
Optimizing Efficiency
Efficiency is a significant factor in the resolution equation; efficiency can be increased by:
• Increasing column length
• Decreasing particle size
Optimizing Pressure
Pressure is most heavily influenced by linear velocity, column length and particle size. Pressure can be decreased by:
• Decreasing column length
• Increasing particle diameter
Performance Equation
a = Selectivity
N = Efficiency
k = Capacity
Performance Efficiency Pressure
A winning balanced solution for fast LC will significantly reduce chromatographic run time while properly addressing the performance requirements dictated by a laboratory’s research objectives (i.e. efficiency, speed, selectivity) and available HPLC systems.
The truth is speed is only one feature of a balanced performance solution. Efficiency and selectivity have critical roles as well. All of these features are interrelated, and manipulation to optimize one feature will affect the others either directly or inversely. Furthermore, they may all contribute towards high system pressure, an undesirable side effect.
�www.phenomenex.com
ContentsThe Truth about Fast LC 2
Balanced Solutions 3
High Speed Technology (HST) Products 4-7
Mercury LC/MS 8-9
Onyx Monolithic Columns 10-11
LC/MS System Optimization 12-13
Ordering Information 14
Speed Pressure Efficiency Selectivity
1 High Speed Technology (HST) Columns Fast < 400 Bar Highest Several phases
2 MercuryMS Columns and Cartridges Fast < 400 Bar High Most phases
3 Monolithic Columns Fastest < �00 Bar Good Some phases
When you want Fast LC, you need BALANCEThe ever-increasing demand for high-throughput analysis of drug candidates during the early stages of drug discovery has generated an acute need for rapid methods of analysis.
3 Balanced Solutions to Balance Your Speed, Pressure, Efficiency and Selectivity
Developing ultra-fast and efficient methods for potential drugs has become a constant challenge for analysts. Use the chart above to determine the HPLC column that meets your performance needs.
It’s really as easy as 1 - 2 - 3
Fast LC
0 10 20 30 min
RS = 24
0 10 20 min
RS = 24
0 5 10 min
RS = 23
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Conditions: (same for all columns)Column: Luna C18(�), particle size as noted
Dimensions: as notedMobile Phase: A: Water - B: Acetonitrile
Gradient: 90:10 (A/B) to 5:95 (A/B)Flow Rate: As notedDetection: UV @ �70 nm
Sample: Ketones C� to C16
Luna 5 μm C18(�) 250 x 4.6 mm Flow Rate: 1.5 mL/min
Luna �.5 μm C18(�)-HST 100 x 2.0 mm Flow Rate: 0.65 mL/min
Luna � μm C18(�) 150 x 4.6 mm Flow Rate: 1.5 mL/min
Run time reduced by 20 min with virtually no effect on resolution!
HST Columns: 66 % Faster. No Loss in ResolutionHST Columns
• High efficiency �.5 µm particles on ultra-pure silica
• Ultra-high performance results on your current HPLC
• Easy method transfer
• Orthogonal selectivity options
HST columns are manufactured in specific dimensions utilizing new, highly controlled and robust packing technologies. The technology allows for consistent, high performance results on newer and existing HPLC instrumentation. Get the benefit of increased speed and efficiency with standard HPLC system pressure capabilities! HST can be used with your current standard HPLC and newer high performance systems so that there will be no need for time consuming method revalidation.
HST �.5 µm columns allow the scientist to reduce analysis time by increasing flow rates without a loss in performance. (See Van Deemter plot on page 6.)
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Speed Influence on Performance
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Faster Cycle Times
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Conditions: (same for all columns)Columns: Luna �.5 μm C18(�)-HST
Dimensions: As notedMobile Phase: A: Water
B: AcetonitrileGradient: 90:10 (A/B) to 5:95 (A/B)
Flow Rate: As notedDetection: UV @ �70 nm
Sample: Ketones C� to C16
0 2 4 6 8 10 min
RS = 23
0 2 4 6 min
RS = 16
0 2 min
RS = 12
100 x 2.0 mm Flow Rate: 0.65 mL/min
50 x 2.0 mm Flow Rate: 0.65 mL/min
50 x 2.0 mm Flow Rate: 1.0 mL/min
2 minute run with less than 400 bar backpressure!
HST Balanced Performance
Speed Pressure Efficiency Selectivity
Run ultra-high performance separations on your current system! With approximately half the backpressure of sub-� µm particles and nearly the same efficiency, Luna �.5 µm C18(�)-HST is a great choice for optimizing your methods.
Conditions: (same for both columns)Columns: Luna �.5 μm C18(�)-HST
Competitor A 1.7 μm Dimensions: 100 x �.0 mm (Luna)
100 x �.1 mm (Competitor A)Mobile Phase: Acetonitrile/Water (65:�5)
Flow Rate: 0.�5 mL/minDetection: UV @ �54 nm
Temperature: �5 °CSample: 1. Uracil
�. Acetophenone
�. Benzene
4. Toluene
5. Naphthalene
10
0
2
3 4
5
2
34
5
2 3 min
1 2 3 min
1
1
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Luna 2.5 µm C18(2)-HST
Competitor A 1.7 μm C18Nearly half the backpressure!
Linear Velocity (mm/s) = Speed
0
400 Bar System Pressure: 600 Bar 1000 Bar
Specialized system needed for faster linear velocity
Specialized system needed for faster linear velocity
2 4 6 8 10 12
Competitor B C181.8 µm
Competitor A C181.7 µm
Luna 2.5 µm C18(2)-HST
254 Bar
435 Bar
Ultra-High Performance without the Backpressure!
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Ap
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Ap
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Fast LC
0
5
10
15
20
25
30
0 1 2 3 4 5 6 7 8
Pla
te H
eigh
t (µ
m)
Competitor A 1.7 µm C18
Luna 2.5 µm C18(2)-HST
Linear Velocity (mm/s)
Flow Rate: 0.55 mL/min
0
1
2
3 4
5
0.4 0.8 min
Competitor A 1.7 µm C18400 barFlow Rate: 0.55 mL/min
0
1
2
3 45
0.4 0.8 min
Luna 2.5 µm C18(2)-HST254 bar
Flow Rate: 1.00 mL/min 400 bar
0.20
1
2
3 45
0.4 0.6 min
Luna 2.5 µm C18(2)-HST Competitor A 1.7 µm C18Flow Rate: 1.00 mL/min
0
2
1
3 45
0.2 0.4 0.6 min
850 bar
Efficiency Influence on Performance
1.9 µm
2.5 µm HST
1.8 µm
1.7 µm
Particle Size:
0
2
4
6
8
10
12
14
16
0 10 20 30 40Reduced Linear Velocity
Red
uced
Pla
te H
eigh
t
Van Deemter equation normalized for particle size
Knox Plot
High Performance at increased linear velocities
Conditions
Columns: Luna �.5 µm C18(�)-HST
Competitor A 1.7 µm C18
Dimension: 50 x �.0 mm (Luna)
50 x �.1 mm (Competitor A)
Mobile Phase: Acetonitrile/Water (65:�5)
Flow Rate: As noted
Detection: UV @ �54 nm
Temperature: �0 ºC
Sample: 1. Uracil
�. Acetophenone
�. Benzene
4. Toluene
5. Naphthalene
HE
TP
Pre
ssur
e
Linear Velocity
HETP = 2 dp + 2 Dm + dp2 v
v Dm
The Van Deemter Equation
The Van Deemter Theory, along with knowledge of the formula here, allows the analyst to visually “experience” the effects of column packing. The relationship between particle size, column length, backpressure, linear velocity and selectivity can be managed so that the result is ideally suited to the specific needs of the analytical chemist.A
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Selectivity Influence on Performance
Conditions
Columns: As notedDimensions: 50 x �.0 mm
Mobile Phase: A. 0.1% Formic Acid in Water (v/v)
B. 0.1% Formic Acid in Acetonitrile (v/v)
Flow Rate: 1.1 mL/minGradient: A/B (95:5) to A/B (5:95) in �.9 min
Temperature: 50 °CDetection: UV @ �54 nm
Sample: 1. Pyridine (0.�� mg/mL)
�. Acetaminophen (0.�0 mg/mL)
�. Benzyl Alcohol (0.�� mg/mL)
4. Nortriptyline (0.5 mg/mL)
5. �-Methyl-4-Nitrobenzoic Acid (0.�5 mg/mL)
6. 4-Chlorcinnamic Acid (0.�0 mg/mL)
7. �-Hydroxy-�-Methylbenzaldehyde (0.�5 mg/mL)
8. Hexanophenone (1.� mg/mL)
All compounds diluted in methanol
0 1 2 min
0
200
400
600
mAU
1
2
3
4 56
7
8
Phenomenex® Luna® 2.5 µm C18(2)-HST 50 x 2.0 mm
0 1 2 min
0
200
400
600
mAU
1
2
3
5
8
4
7 6
Phenomenex® Synergi™ 2.5 µm Polar-RP HST 50 x 2.0 mm
0 1 2 min
0
200
400
600
mAU
1
2
3
4 56
7
8
Phenomenex® Synergi™ 2.5 µm Fusion-RP HST 50 x 2.0 mm
0 1 2 min
0
200
400
600
mAU
1
2
3
5
4
67
8
Thermo® Hypersil™ GOLD™ C18 1.9 µm 50 x 2.1 mm
0 1 2 min
0
200
400
600
mAU
1
2
3
4,5 67
8
Agilent® Zorbax® Eclipse XDB-C18 RRHT 1.8 µm 50 x 2.1 mm
Speed of analysis is dependant upon more than just column length and particle size.
• The resolution of one or more critical compounds in a multi-analyte sample can dictate the ultimate maximum speed you may obtain.
• Selectivity is the strongest factor in the resolution equation, and is influenced heavily by stationary phase and silica purity.
• By optimizing selectivity to increase baseline resolution, the analyst has more opportunity to reduce run times.
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Hydrophobic Selectivity (see page 9)
Polar Selectivity (see page 9)
Balanced Selectivity (see page 9)
Fast LC
Column: Synergi �.5 µm Fusion-RP MercuryMS™
Dimensions: 10 x �.0 mm cartridgeGradient: 15:85 to 95:5 % B in 1.0 min, re-equilibration for 1.0 min (�.0 min cycle)Injection: 10 µL of 100 ng/mL Benzodiazepines in urine after SPE clean-up
Flow Rate: 0.6 mL/minPart No.: 00N-44��-B0-CE
Mobile Phase: A: 0.1% Formic Acid in Water
B: 0.1% Formic Acid in Acetonitrile†Full method, recovery and RSD data available, please request technical note: TN-10�0, High Speed
LC/MS Analysis with MercuryMS Cartridges for High-Throughput Drug Discovery.
Benzodiazepines in Urine (LC/MS/MS - 2)
0.5 1 1.5 min0.00
1.00e5
2.00e5
3.00e5
4.00e5
5.00e5
6.00e5
7.00e5
8.00e5
9.00e5
1.00e6
Inte
nsity
, cp
s
0.43
0.67
0.5 1 1.5 min0.0
1.0e5
2.0e5
2.7e5
0.29
284.2/135.2
0.5 1 1.5 min0.0
1.0e5
2.0e52.4e5
0.43
0.67
300.3/227.0
0.5 1 1.5 min0.0
4.0e5
0.75
271.2/140.2
0.5 1 1.5 20.0
5.0e5
7.4e5
0.76
287.0/241.2
0.5 1 1.5 min0.0
1.0e5
2.0e52.6e5 0.81
316.1/270.1
0.5 1 1.5 min0.00
5.00e5
1.00e60.82
301.2/255.0
0.5 1 1.5 min0.0
2.0e5
4.0e50.84
314.2/268.3
0.5 1 1.5 min0.0
1.0e5
2.0e5
3.0e53.9e5
0.85
285.2/193.2
7-Aminoflunitrazepam Chlordiazepoxide
Nordazepam Oxazepam
Clonazepam Temazepam
Flunitrazepam Diazepam
Retain 8 Polar Compounds in Less than 1 Minute!
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• Ultra-fast, low-cost analysis for high-throughput laboratories
• Packed with Luna®, Synergi™, and Gemini® material
• Short 10 and �0 mm cartridge formats use a new proprietary slurry packing process
MercuryMS: Analytical Column Performance in Small Columns and Cartridges
Traditional LC System!
Max Pressure: 5000 psi (�44 bar)Max Flow: �.0 mm ID: 1.� mL/min
4.0 mm ID: 4 mL/min
8 www.phenomenex.com
9www.phenomenex.com
MercuryMS Balanced Performance
Speed Pressure Efficiency Selectivity
Rugged Durability for over 1,000 Injections
Column: Luna � μm C18(�) Dimensions: �0 x �.0 mm MercuryMS Cartridge
Part No.: 00M-4�51-B0-CEMobile Phase: A: Water with 0.1 % Trifluoroacetic acid (TFA)
B: Methanol with 0.1 % TFAGradient: 95:5 A/B to 5: 95 A/B in � min
Flow Rate: 0.4 mL/minDetection: UV @ ��0 nm
Temperature: AmbientSample: � µL containing:
1. Propranolol
�. Metoprolol
�. Pindolol
Injection#
3.00
2.75
2.50
2.25
2.00
1.75
1.50
1.25
1.00
0.75
0.50
Ret
entio
n tim
e (m
in) o
r S
ymm
etry
tR #1tR #2tR #3
Symmetry #1Symmetry #2Symmetry #3
10008006004002000
Selecting a Phase to Maximize
LC/MS Sensitivity
Synergi™ Hydro-RP A polar-endcapped C18 that offers extreme retention of
hydrophobicandhydrophiliccompounds.pHstablefrom1.5to7.0(2.5μm,4μm)
Luna® C18(2) Our most popular C18 material with high hydrophobic
retention and exceptional 1.5 to 10 pH stability. (2.5 µm,3μm,5μm)
Synergi™ Max-RP (C12withTMSendcapping) -Givesexcellentpeakshape
forbasicanalytes,particularlyatneutralpH,andisintendedtobeusedwithavarietyofMS-compatiblemobilephasemodifierssuchasTFA,formicacid,oraceticacid.pHstablefrom1.5to10.(2.5μm,4μm)
Gemini® C18 TWIN™ Technology combines the best of silica and
polymersforwidepHstability(pH1-12)andhighefficiency.Exceptionalretentionandpeakshapeofbasiccompounds.(3μm,5μm)
Synergi™ Fusion-RP ApolarembeddedC18thatoffersabalancedapproachin
separationofnon-polartopolarcompounds.pHstablefrom1.5to10.(2.5μm,4μm)
Luna® C8(2) Amid-rangephasethatisanexcellentstartingpointforthe
methoddevelopmentofcomplexmixtures.pHstablefrom1.5to10.(3μm,5μm)
Gemini® C6-Phenyl pHstable1-12TWIN™Technologyallowsforamoreinert,
reproducible phenyl phase. Mixed-mode hydrophobic andaromaticinteractionsmakesthisanexcellentphaseforpolarmetabolites.(3μm,5μm)
Synergi™ Polar-RP An ether-linkedphenyl phasewith proprietary hydrophilic
endcapping.This column has maximum selectivity andretentionforpolararomaticcompounds.(2.5µm,4µm)Low
High
Reduce Analysis Times by 60 %
Column: Luna � μm C18(�)Dimensions: 50 x 4.6 mm, �0 x 4.6 mm, �0 x 4.0 mm
Mobile Phase: A: 0.1 % Formic acid in Water B: 0.1 % Formic acid in Acetonitrile
Gradients: 95:5 A/B to 5:95 in 6 min for 50 x 4.6 mm 95:5 A/B to 5:95 in 4 min for �0 x 4.6 mm 95:5 A/B to 5:95 in �.4 min for �0 x 4.0 mm
Flow Rate: � mL/min for 50 and �0 x 4.6 mm,
1.6 mL/min for �0 x 4.0 mmDetection: UV @ �54 nm
Temperature: Ambient
Sample: 5 µL Gradient mixture
1. Acetaminophen
�. Propranolol
�. Imipramine
4. Naproxen
5. Valerophenone
0 2 4 min
0 2 4 min
4 min20
50 x 4.6 mm Column
30 x 4.6 mm Column
20 x 4.0 mm Cartridge
vs.
vs.60 % Reduction in Run Time
Hyd
rop
hobic
ity
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Fast LC
4 runs
4 runs
Who Will Finish First?Fast equilibration and shorter run times allow more runs per hour than traditional columns.
Fast re-equilibration
In a research environment, it is very common to run multiple samples under varied conditions. Therefore, the total working time of the column is not just the run time, but also the period required to re-equilibrate the column between solvent gradient runs. Using Onyx you will not only dramatically reduce run time, but also equilibration time resulting in increased lab throughput.
Onyx monolithic column
Traditional particle-based column
Equilibration Time
Separation Time
Column: Onyx Monolithic C18Dimension: 100 x 4.6 mm
Part No.: CH0-764�Mobile Phase: 0.1 % TFA in water /
Acetonitrile (95/5, v/v)
Flow Rates: 1 mL/min to 4 mL/minDetection: UV @ ��0 nm
Temperature: �� °CSample: 1. Maleic Acid
�. Fumaric Acid
1
2
0 1 2 3 min
1
2
0 1 2 3 min
1
2
0 1 2 3 min
0 1 2 3 min
1
21 mL/min
(�0 bar)
2 mL/min(61 bar)
3 mL/min(95 bar)
4 mL/min(1�0 bar)
Ap
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155
10A
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ID 1
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10 www.phenomenex.com
Cut run times by more than half!
Results in 1/4 the time!
Finish First With Onyx Monolithic Silica HPLC Columns
The monolithic nature of Onyx gives scientists opportunities not common with particle-based columns.
• Reduce run times by more than 50 %
• Rapid screening and increased sample throughput
• “Dilute-and-Shoot” dirty biological samples
• Extremely high efficiencies without backpressure limitations
• Available in C18, C8, and Si phases
• Analytical, capillary, and semi-prep dimensions
11www.phenomenex.com
Onyx Balanced Performance
Speed Pressure Efficiency Selectivity
0 1 2 30
1
1
2
2
Intens. x 105
min
Ap
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88
Sample Preparation:
Human urine sample diluted 1:1 with water. 50 µL Injected.
Column: Onyx Monolithic C18Dimensions: 100 x 4.6 mm
Part No.: CH0-764�Mobile phase A: 0.1 % Formic acid in Water
B: 0.1 % Formic acid in AcetonitrileGradient: 5 to 90 % B in 4.0 min
Flow rate: 4.0 mL/minTemperature: �0 °C
Detection: LC/MS ESI+Sample: 1. Nordiazepam
(Diazepam metabolite) (m/z = �71)
�. Diazepam (m/z = �85)
Anti-Hypertensives in plasma
Background: Several related drug compounds in plasma sample. Samples precipitated with organic and run through a protein precipitation plate (PPT).
Challenge: Maintain compound retention, separation, and peak shape even though sample is in 80 % organic.
Solution: Macroporous structure of Onyx mixes solvent slug quickly. Compound retains on phase and still maintains excellent separation.
Challenge: Avoiding flow restrictions and overpressures due to salts, unprecipitated proteins, sugars, and lipids present in the sample.
Solution: The low backpressure of Onyx makes overpressures unlikely even at very high flow rates. Also, the through-pores of the media make Onyx less likely to “plug up” from matrix contaminants versus particulate columns.
Diazepam and metabolite in urine
Background: Monitoring of a pharmaceutical compound and its metabolite in urine. Sample is directly injected via “dilute-and-shoot” method onto column.
Challenge: “Dilute-and-shoot” contains large amounts of proteins as well as other contaminants that can potentially clog the column quickly.
Solution: Wide macropores (� µm) of Onyx reduce interference due to proteins present in sample and allow sample to easily pass through column.
Challenge: High-throughput method needed with short run time.
Solution: Monolithic structure allows for rapid gradients with very short re-equilibration times resulting in methods less than 4 minutes.
Challenge: Sample carryover can be problematic with complex samples.
Solution: Improved flow characteristics of monoliths result in lower sample carryover, especially for complex matrices like urine and plasma.
0 1 2 3 min0
1
1
2
34
2
Intens. x 105
Ap
p ID
155
87
Sample Preparation:
A �00 µL sample of porcine plasma was spiked with � µg/mL drug mixture and then precipitated with 800 µL acetonitrile and filtered through a Phenomenex Impact Protein Precipitation Plate
Column: Onyx Monolithic C18Dimensions: 100 x 4.6 mm
Part No.: CH0-764�Mobile phase: A: 0.1 % Formic acid in Water
B: 0.1 % Formic acid in AcetonitrileGradient: 10 to 65 % B in 4.0 min
Flow rate: 4.0 mL/minTemperature: �0 °C
Detection: LC/MS ESI+Sample: 1. Pseudoephedrine (m/z = 166)
�. Propranolol (m/z = �60)
�. Diltiazem (m/z = 415)
4. Verapamil (m/z = 455)
Fast LC
�
1HPLC System Configurations
BEFORE Optimization Fast LC AFTER Optimization
HPLC System: HP 1100 series (www.agilent.com) Same
Pump: G1�1�A (Binary Pump) without in-line mixer
Same
Detector: G1�15A DAD with standard flow cell 10 mm path (13 μL)
G1�15A DAD with semi-micro flow cell 6 mm path (5 μL)
Injector (Autosampler):
G1��9A ALS with Needle Seat Capillary (0.17 mm ID – green)
G1��9A ALS with Needle Seat Capillary (0.12 mm ID – red)
Connecting tubing
Pump to Injector: 55 cm x 0.17 mm (green PEEK tubing)
55 cm x 0.125 mm (red PEEK tubing)
Needle seat: 0.17 mm ID 2.3 μL (green PEEK tubing)
0.12 mm ID, 1.2 μL (red PEEK tubing)
Injector to Column:
28 cm x 0.17 mm (yellow PEEK tubing)
�8 cm x 0.1� mm (red PEEK tubing)
Column to DAD: �8 cm x 0.1� mm (red PEEK tubing) Same
MS Conditions
MS Detector: — API �000 LC/MS/MS System (www.appliedbiosystems.com), ESI+ (TurboIonSpray), MRM
Tubing from Column to MS
detector:
— 5� cm x 0.1� mm (red PEEK tubing)
TurboIonSpray heater gas flow:
— 6500 cc/min
TurboIonSpray heater
temperature:
— 4�5 - 450 ˚C
If you do nothing else...
The default setting for the detector time constant is � seconds. Decrease this to
the minimum 0.1� seconds and use a �.0 mm HST column for best results.
Simple, fast company-wide implementation
Balanced Fast LC Means...
easy company-wide implementation
easy method transfer
easy interface with orthogonal detection techniques
you can start today
using your current instrumentation
Methods can be successfully and quickly validated on Fast LC
Specificity Demonstrate resolution of main peak from available
impurities and degradants
Injection repeatability Six injections of one prep of working standard solution
Peak area % RSD < �.0 % for the main peak
Linearity Evaluate linearity from 50 % to 150 % of working
concentration R� ≥ 0.995, % Y-intercept < �.0 %
Sensitivity Evaluate sensitivity at the reporting limit (0.0� %)
S/N ≥ 10, Peak area % RSD < 15 % (n ≥ 6)
No additional instrumentation or training required!
Do it yourself system for Fast LC Phenomenex’s Fast LC Solutions are as easy as 1 - 2 - 3
1� www.phenomenex.com
1�www.phenomenex.com
�Resources for FAST LC Optimization
Phenomenex Technical Department
For personal assistance in optimizing your
methods and system for fast LC, please
contact your Phenomenex technical consul-
tant or email [email protected]
Request any of the technical note
and white papers from your
Phenomenex technical consultant
or through the website at
www.phenomenex.com
Technical Note 1030
High Speed LC/MS Analysis
with MercuryMS Cartridges for
High-Throughput Drug Discovery
Technical Note 1032
Optimizing Performance for Fast
HPLC Analysis on Short Columns
Technical Note 1031
Improved Results for LC/MS of
Basic Compounds Using High pH
Mobile Phase on a Gemini® C18
Column
Technical Note 1024
Advantage of Silica Monolithic
HPLC Columns – Highly Repro-
ducible Results with Onyx™
Technical Note 1033
Direct Plasma Analysis of Drug
Compounds Using Onyx
Monolithic Columns
White Paper
Advantages of �.5 µm for
increasing the speed of
analysis while maintaining
high efficiency
Fast LC
Ordering Information
High Speed Technology (HST) 2.5 μm Luna and Synergi Columns
Phase 30 x 2.0 mm 50 x 2.0 mm 100 x 2.0 mm 50 x 3.0 mm 100 x 3.0 mm 50 x 4.6 mm
Luna 2.5 μm C18(2)-HST 00A-4446-B0 00B-4446-B0 00D-4446-B0 00B-4446-Y0 00D-4446-Y0 —
Synergi 2.5 μm Max-RP HST 00A-4372-B0 00B-4372-B0 00D-4372-B0 00B-4372-Y0 00D-4372-Y0 00B-4372-E0
Synergi 2.5 μm Polar-RP HST 00A-4371-B0 00B-4371-B0 00D-4371-B0 00B-4371-Y0 00D-4371-Y0 00B-4371-E0
Synergi 2.5 μm Hydro-RP HST 00A-4387-B0 00B-4387-B0 00D-4387-B0 00B-4387-Y0 00D-4387-Y0 00B-4387-E0
Synergi 2.5 μm Fusion-RP HST 00A-4423-B0 00B-4423-B0 00D-4423-B0 00B-4423-Y0 00D-4423-Y0 00B-4423-E0
* 4 μm Synergi materials available upon request.
MercuryMS™ Cartridges and Columns
Cartridges (mm) Columns (mm)
Phase 10 x 2.0 10 x 4.0 20 x 2.0 20 x 4.0 20 x 2.0 20 x 4.0
2.5 μm
Synergi Hydro-RP* 00N-4387-B0-CE 00N-4387-D0-CE 00M-4387-B0-CE 00M-4387-D0-CE 00M-4387-B0 00M-4387-D0
Synergi Max-RP* 00N-4372-B0-CE 00N-4372-D0-CE 00M-4372-B0-CE 00M-4372-D0-CE 00M-4372-B0 00M-4372-D0
Synergi Fusion-RP* 00N-4423-B0-CE 00N-4423-D0-CE 00M-4423-B0-CE 00M-4423-D0-CE 00M-4423-B0 00M-4423-D0
Synergi Polar-RP* — 00N-4371-D0-CE 00M-4371-B0-CE 00M-4371-D0-CE 00M-4371-B0 00M-4371-D0
3 μm
Luna C18(2) 00N-4251-B0-CE 00N-4251-D0-CE 00M-4251-B0-CE 00M-4251-D0-CE 00M-4251-B0 00M-4251-D0
Luna C8(2) 00N-4248-B0-CE 00N-4248-D0-CE 00M-4248-B0-CE 00M-4248-D0-CE 00M-4248-B0 00M-4248-D0
Gemini C6-Phenyl 00N-4443-B0-CE 00N-4443-D0-CE 00M-4443-B0-CE 00M-4443-D0-CE 00M-4443-B0 00M-4443-D0
Gemini C18 Inquire Inquire 00M-4439-B0-CE 00M-4439-D0-CE 00M-4439-B0 00M-4439-D0
5 μm
Luna C18(2) 00N-4252-B0-CE 00N-4252-D0-CE 00M-4252-B0-CE 00M-4252-D0-CE — —
Luna C8(2) 00N-4249-B0-CE 00N-4249-D0-CE 00M-4249-B0-CE 00M-4249-D0-CE — —
Gemini C18 — — 00M-4435-B0-CE 00M-4435-D0-CE — —
MercuryMS Standard Cartridge Holders
Part No. Description
CH0-5846 10mmstandardholder
CH0-5845 20mmstandardholder
MercuryMS Direct-Connect Cartridge Holders
Part No. Description
CH0-7187 10mmdirect-connectholder
CH0-7188 20mmdirect-connectholder
Onyx™ Monolithic Columns
Part No. Description Size (mm)
Capillary Columns
CH0-7646 OnyxMonolithicC18 150x0.1
Analytical Columns
CH0-8158 OnyxMonolithicC18 100x3.0
CH0-7643 OnyxMonolithicC18 100x4.6
CH0-7644 OnyxMonolithicC18 50x4.6
CH0-7645 OnyxMonolithicC18 25x4.6
CH0-7647 OnyxMonolithicC8 100x4.6
CH0-7648 OnyxMonolithicSi 100x4.6
Semi-Prep Columns
CH0-7878 OnyxMonolithicC18 100x10.0
Guard Cartridge System
KJ0-7651 OnyxMonolithicC18GuardCartridgeKit(3pkcartridges+holder+wrench)
5x4.6
CH0-7649 OnyxMonolithicC18GuardCartridges(3/pk) 5x4.6
KJ0-7652 OnyxMonolithicC18GuardCartridgeKit(3pkcartridges+holder+wrench)
10x4.6
CH0-7650 OnyxMonolithicC18GuardCartridges(3/pk) 10x4.6
Method Validation Kit
KH0-7653 OnyxMonolithicC18MethodValidationKit(3columnsfromdifferentbatches)
100x4.6
Column Coupler
AQ0-7654 OnyxColumnCoupler
Product based on monolithic technology under license from Merck KGaA, Darmstadt, Germany
14 www.phenomenex.com
15www.phenomenex.com
2.5 µm High Speed Technology (HST) columns
Onyx Monolithic columns
MercuryMS LC/MS columns and cartridges
If you are not completely satisfied with any of the Phenomenex products featured in this guide,
RETURN WITHIN 45 DAYS FOR A FULL REFUND.
HST Balanced Performance
Speed Pressure Efficiency Selectivity
Onyx Balanced Performance
Speed Pressure Efficiency Selectivity
MercuryMS Balanced Performance
Speed Pressure Efficiency Selectivity
Luna and Gemini are registered trademarks of Phenomenex, Inc. Synergi, Polar-RP, Onyx, MercuryMS, and TWIN Technology are trademarks of Phenomenex, Inc. Hypersil and GOLD are trademarks of Thermo Scientific, a part of Thermo Fisher Scientific, Inc. Zorbax is a registered trademark of Agilent Technologies. Phenomenex, Inc. is in no way affiliated with Thermo Scientific, Thermo Fisher Scientific, or Agilent Technologies. Comparative separations may not be representative of all applications. © �007 Phenomenex, Inc. All rights reserved.
Fast LC
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