FIELD TRIAL REPORT · FIELD TRIAL REPORT Efficacy and selectivity of Anolyte (AcquAttiva®) to control Erwinia amylovora causing fireblight on BIO pear PROTOCOL ID:1062018G_B

FIELD TRIAL REPORT

Efficacy and selectivity of Anolyte (AcquAttiva®) to control Erwinia amylovora

causing fireblight on BIO pear

PROTOCOL ID:1062018G_B SATA TRIAL ID: 1062018G_B_01

Sponsor: Bio Fruit Service Via Savigliano, 75/A 12024 Costigliole Saluzzo (CN) - Acqua Attiva 20875 Burago di Molgora (MB)

Quargnento, 28/11/2019

Innovare e crescere insieme

PROTOCOL ID: 1062018G_B SATA TRIAL ID: 1062018G_B_01

Efficacy and selectivity of Anolyte (AcquAttiva®) to control Erwinia amylovora causing fireblight on

BIO pear

Pag. 2 di 38

Pagina 2 di 38

SATA S.r.l. – Strada Alessandria, 13 – 15044 Quargnento (AL) - Tel 0131219925 - Fax 0131219926 –

e-mail: [email protected] – web: www.satasrl.it

CONTENTS

CONTENTS .................................................................................................................................... 1

1 GEP COMPLIANCE STATEMENT ........................................................................................... 3

2 GEP OFFICIAL ACCREDITATION ........................................................................................... 4

3 ARCHIVING STATEMENT ....................................................................................................... 6

4 SUMMARY ............................................................................................................................... 6

5 TRIAL INFORMATION & METHODS ....................................................................................... 7

5.1 TRIAL TREATMENTS ............................................................................................................ 7 5.2 GENERAL TRIAL INFORMATION ............................................................................................ 7 5.3 TRIAL LOCATION ................................................................................................................. 7 5.4 CONTACTS ......................................................................................................................... 8 5.5 COOPERATOR/LANDOWNER ................................................................................................ 8 5.6 CROP DESCRIPTION ........................................................................................................... 8 5.7 PEST DESCRIPTION ............................................................................................................ 8 5.8 SITE AND DESIGN ............................................................................................................... 9 5.9 FIELD PLOT LAYOUT ............................................................................................................ 9 5.10 APPLICATION DESCRIPTION ............................................................................................... 10 5.11 CROP STAGE AT EACH APPLICATION ................................................................................. 10 5.12 PEST STAGE AT EACH APPLICATION .................................................................................. 11 5.13 APPLICATION EQUIPMENT ................................................................................................. 11

6 MOISTURE AND WEATHER CONDITIONS .......................................................................... 12

7 STATISTICAL ANALYSIS METHODS .................................................................................... 12

8 RESULTS ............................................................................................................................... 13

8.1 EFFICACY ......................................................................................................................... 13 8.2 PHYTOTOXICITY ................................................................................................................ 13

9 FINAL REMARKS................................................................................................................... 14

10 ANNEX ............................................................................................................................... 15

10.1 RAW DATA ........................................................................................................................ 15 10.2 RAW WEATHER DATA ........................................................................................................ 25 10.3 CHARTS ........................................................................................................................... 27 10.4 STATISTICAL ANALYSIS ...................................................................................................... 28 10.5 TRIAL SITE LOCATION ........................................................................................................ 36




BIO pear

Pag. 3 di 38

Pagina 3 di 38



1 GEP COMPLIANCE STATEMENT

The field trial was performed in according with Sponsor study protocol.

The trial was managed according to the OEPP/EPPO Guidelines on Efficacy Evaluation of

Plant Protection Product:

PP 1/166(3) Erwinia amylovora

PP 1/152(4) Design and analysis of efficacy evaluation trials

PP 1/135(3) Phytotoxicity assessment

PP 1/181(4) Conduct and reporting of efficacy evaluation trials including GEP

L.D. March 1995 n.194, accomplishment of Directive 91/414/ECC and Regulation N.

1107/2009.

Internal SOPs.

Study Director: Alessandro Costanzo Date: 28/11/2019

Signature:




BIO pear

Pag. 4 di 38

Pagina 4 di 38



2 GEP OFFICIAL ACCREDITATION




BIO pear

Pag. 5 di 38

Pagina 5 di 38






BIO pear

Pag. 6 di 38

Pagina 6 di 38



3 ARCHIVING STATEMENT

The protocol, raw data and copy of the final report will be archived for ten years in the office GEP

of SATA srl, Strada Alessandria, 13 – 15044 Quargnento (AL) – Italy.

4 SUMMARY

The experimental field trial was carried out in the countryside of Centallo (province of Cuneo –

Northern Italy) from May 2019 to July 2019, in order to evaluate the efficacy and selectivity of

Anolyte (AcquAttiva®) applied on BIO pear to control Erwinia amylovora (ERWIAM).

Anolyte (AcquAttiva®) was been tested in comparison to organic standard control strategy (Amylo X+Vacciplant+Poltiglia Disperss). Disease symptoms were visible at 30/05/2019 but the disease pressure was very low and concentrated on the buds. Anolyte (AcquAttiva®) showed good efficacy to control disease, but efficacy evaluation was not completely verified because the disease pressure was very low. All treatments did not show any phytotoxicity symptoms on the crop. La prova sperimentale è stata condotta a Centallo (provincia di Cuneo – Nord Italia) da Maggio 2019 a Luglio 2019, al fine di valutare l’efficacia e la selettività di Anolyte (AcquAttiva®) per il controllo di Erwinia amylovora (ERWIAM). Anolyte (AcquAttiva®) è stata testata a confronto con una strategia BIO standard (Amylo X+Vacciplant+Poltiglia Disperss). I sintomi della malattia erano visibili al 30/05/2019, ma la pressione era molto bassa e concentrata sui germogli. Anolyte (AcquAttiva®) ha mostrato una buona efficacia nel controllo della malattia, ma la valutazione dell’efficacia non è completamente verificata in quanto la pressione della malattia era molto bassa. Tutti i trattamenti non hanno mostrato alcun sintomo di fitotossicità sulla coltura.




BIO pear

Pag. 7 di 38

Pagina 7 di 38



5 TRIAL INFORMATION & METHODS

5.1 TRIAL TREATMENTS Trt Treatment Form Form Form Specific Rate Appl No. Type Name Conc Unit Type Gravity Description Supplier Rate Unit Code

1 CHK Untreated Check not treated

2 PLHE Anolyte - AcquaAttiva L 450 l/ha ABCDEFGH 3 FUNG Amylo-X 250 gA/kg WG 1 CBC 2 kg/ha ABDF -Bacillus amyloliquefacens 250 500 g AI FUNG Vacciplant 45 gA/L SL 1,05 Ary 1 l/ha CE -Laminarina 45 45 g AI FUNG Poltiglia Disperss 200 gA/kg WG 1 UPL 5 kg/ha GH -Rame metallo 200 1000 g AI

Replications: 4, Untreated treatments: 1, Conduct under GLP/GEP: Yes (GEP with no protection), Design: Randomized Complete Block (RCB), Treatment units: Treated 'Plot' experimental unit size, Dry Form. Unit: %, Treated 'Plot' experimental unit size Width: 4 meters, Treated 'Plot' experimental unit size Length: 9 meters, Application amount: 900 L/ha, Mix size: 14.96 L, Overage: 2000 mL, Format definitions: G-All7.def, G-All7.frm

5.2 GENERAL TRIAL INFORMATION

Study Director: Alessandro Costanzo Title: Dott. Investigator: Ivano RAMON Title: Dott.

Discipline: F fungicide

Trial Status: E established ARM Trial Created On: Mar-25-2019

Initiation Date: Mar-27-2019 Completion Date: Jul-26-2019

Conducted Under GLP: No Conducted Under GEP: Yes

No. Guideline Discipline Description

1. PP 1/181(4) GS Conduct and reporting of efficacy evaluation trials including GEP

2. PP 1/152(4) GS Design and analysis of efficacy evaluation trials 3. PP 1/135(4) GS Phytotoxicity assessment

4. PP 1/166(3) F Erwinia amylovora

Objectives: To evaluate efficacy and selectivity of Anolyte (AcquAttiva®) to control Erwinia amylovora causing fireblight on BIO pear

5.3 TRIAL LOCATION Trial Location

City: Centallo Country: ITA Italy State/Prov.: Cuneo

Postal Code: 12044 Climate Zone: EPOMED EPPO Mediterranean

Latitude of LL Corner °: 44,5081 N

Longitude of LL Corner °: 7,5956 E Altitude of LL Corner: 424,00 m




BIO pear

Pag. 8 di 38

Pagina 8 di 38



5.4 CONTACTS

Study Director: Alessandro Costanzo Title: Dott. Organization: SATA srl

Address: Strada Alessandria 13 Phone No.: +39 0131 219925 City+State/Prov: Quargnento Mobile No.: +39 349 2559462

Postal Code: 15044 E-mail: [email protected] Country: Ita

Investigator: Ivano RAMON Title: Dott.

Organization: SATA srl Address: Strada Alessandria 13 Phone No.: +39 0131 219925

City+State/Prov: Quargnento Mobile No.: +39 346 3906013 Postal Code: 15044 E-mail: [email protected]

Country: Ita Other Contacts

Name Role Other

Marco Capra Tecnician SATA srl

Massimiliano Amerelli Tecnician SATA srl

5.5 COOPERATOR/LANDOWNER Cooperator/Landowner

Cooperator: Società agricola Aia Piccola Role: FALDOW Address 1: Reg. Crosia, 365

City: Centallo State/Prov: CN

Postal Code: 12044 Country: Ita

5.6 CROP DESCRIPTION

Crop 1: C PYUCO Pyrus communis Common pear BBCH Scale: BPOM Stage Scale: BBCH

Variety: Abate Perennial Age: 7 YR

Planting Density: 1250 P/ha Row Spacing: 4 m

Spacing within Row: 2 m

5.7 PEST DESCRIPTION

Pest 1 Type: D Code: ERWIAM Erwinia amylovora Common Name: Fire blight Entry Date: Sep-10-2019




BIO pear

Pag. 9 di 38

Pagina 9 di 38



5.8 SITE AND DESIGN

Treated Plot Width: 4 m Treated Plot Length: 9 m

Treated Plot Area: 36 m2 Treatments: 3 Replications: 4 Study Design: RACOBL Randomized Complete Block (RCB)

5.9 FIELD PLOT LAYOUT

403 1

401 2

402 3

301 1

303 3

302 2

201 3

202 1

203 2

102 3

101 2

103 1




BIO pear

Pag. 10 di 38

Pagina 10 di 38



5.10 APPLICATION DESCRIPTION

A B C D E F G

Application Date Mar-27-2019 Apr-2-2019 Apr-9-2019 Apr-17-2019 Apr-25-2019 May-3-2019 May-22-2019 Appl. Start Time 11:00 11:50 11:20 11:15 10:45 14:20 11:50

Appl. Stop Time 11:30 12:20 12:17 11:40 11:00 14:50 12:20

Application Method SPRAY SPRAY SPRAY SPRAY SPRAY SPRAY SPRAY

Application Timing BBCH 59-60 BBCH 67-69 DAA 7-10 DAA 7-10 DAA 7-10 DAA 7-10 Application Placement FOLIAR FOLIAR FOLIAR FOLIAR FOLIAR FOLIAR FOLIAR

Applied By M. Capra M. Capra M. Capra M. Capra M. Capra M. Capra M. Capra

Appl. Entry Date Sep-10-2019 Sep-10-2019 Sep-10-2019 Sep-10-2019 Sep-10-2019 Sep-10-2019 Sep-10-2019

Air Temperature Start, Stop 13,9 14,5 C 21,8 22,0 C 16,9 15,7 C 23,5 24,8 C 13,2 13,4 C 21,2 21,3 C 23,4 24,8 C % Relative Humidity Start, Stop 41,1 35,2 42,4 41,9 61,6 70,2 44,7 42,8 70,2 71,2 58,2 59,2 59,2 59

Wind Velocity+Dir. Start 0 KPH 0 KPH 2,6 KPH SE 0 KPH 0 KPH 5,6 KPH SW 0 KPH

Wind Velocity+Dir. Stop 0 KPH 0 KPH 3,2 KPH SE 0 KPH 0 KPH 4,8 KPH SW 0 KPH

Wind Velocity+Dir. Max 0 KPH 0 KPH 3,2 KPH SE 0 KPH 0 KPH 5,6 KPH SW 0 KPH Wet Leaves (Y/N) N no N no N no N no N no N no N no

Soil Moisture DRY DRY WET WET WET NORMAL NORMAL

% Cloud Cover 0 20 0 0 80 90 10

H

Application Date May-30-2019

Appl. Start Time 11:25

Appl. Stop Time 12:00 Application Method SPRAY

Application Timing

Application Placement FOLIAR

Applied By M. Capra Appl. Entry Date Sep-10-2019

Air Temperature Start, Stop 24,9 25,0 C

% Relative Humidity Start, Stop 39,7 38,6 Wind Velocity+Dir. Start 3,1 KPH E

Wind Velocity+Dir. Stop 6,3 KPH E

Wind Velocity+Dir. Max 6,3 KPH E

Wet Leaves (Y/N) N no Soil Moisture NORMAL

% Cloud Cover 0

5.11 CROP STAGE AT EACH APPLICATION

A B C D E F Crop 1 Code, BBCH Scale PYUCO BPOM PYUCO BPOM PYUCO BPOM PYUCO BPOM PYUCO BPOM PYUCO BPOM

Stage Scale Used BBCH BBCH BBCH BBCH BBCH BBCH

Stage Majority, Percent 59 65 65 69 71 72

Stage Minimum, Percent 59 65 65 69 71 72 Stage Maximum, Percent 60 65 67 69 71 72

G H Crop 1 Code, BBCH Scale PYUCO BPOM PYUCO BPOM

Stage Scale Used BBCH BBCH

Stage Majority, Percent 73 74

Stage Minimum, Percent 73 74 Stage Maximum, Percent 73 74




BIO pear

Pag. 11 di 38

Pagina 11 di 38



5.12 PEST STAGE AT EACH APPLICATION

A B C D E F G H

Pest 1 Code, Type, Scale ERWIAM D ERWIAM D ERWIAM D ERWIAM D ERWIAM D ERWIAM D ERWIAM D ERWIAM D Stage Majority, Percent PRINFC PRINFC PRINFC PRINFC PRINFC PRINFC PRINFC PRINFC

Stage Minimum, Percent PRINFC PRINFC PRINFC PRINFC PRINFC PRINFC PRINFC PRINFC

Stage Maximum, Percent PRINFC PRINFC PRINFC PRINFC PRINFC PRINFC PRINFC PRINFC

5.13 APPLICATION EQUIPMENT

A B C D E F G H

Equipment Type KNAMOT KNAMOT KNAMOT KNAMOT KNAMOT KNAMOT KNAMOT KNAMOT

Operation Pressure 10 BAR 10 BAR 10 BAR 10 BAR 10 BAR 10 BAR 10 BAR 10 BAR

Nozzle Type CONSOL CONSOL CONSOL CONSOL CONSOL CONSOL CONSOL CONSOL Nozzle Size 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5

Boom ID SataNord082 SataNord082 SataNord082 SataNord082 SataNord082 SataNord082 SataNord082 SataNord082

Carrier WATER WATER WATER WATER WATER WATER WATER WATER

Application Amount 900 L/ha 900 L/ha 900 L/ha 900 L/ha 900 L/ha 900 L/ha 900 L/ha 900 L/ha Mix Overage 2000 mL 2000 mL 2000 mL 2000 mL 2000 mL 2000 mL 2000 mL 2000 mL

Mix Size 14,96 L 14,96 L 14,96 L 14,96 L 14,96 L 14,96 L 14,96 L 14,96 L




BIO pear

Pag. 12 di 38

Pagina 12 di 38



6 MOISTURE AND WEATHER CONDITIONS

Closest Weather Station: Centallo Distance: 5 km

Chart 1 - Temperatures and rainfall during trial

(Source: Rete Agrometeorologica del Piemonte - Regione Piemonte - Assessorato Agricoltura - Settore Fitosanitario - Sezione di Agrometeorologia)

7 STATISTICAL ANALYSIS METHODS

Data collected during the trial were subjected to analysis of variance ANOVA. Treatment average

means were calculated and were compared using the TUKEY HSD test (p=0.05). Statistical

procedures were applied using the ARM 2019 from Gylling Data Management.




BIO pear

Pag. 13 di 38

Pagina 13 di 38



8 RESULTS

8.1 EFFICACY Efficacy evaluation was carried out counting the number of buds infected by ERWIAM. The buds infected was been removed on each plots. At first assessment Anolyte (AcquAttiva®) showed good efficacy to control disease, but Bio standard strategy showed higher efficacy (Chart 2, paragraph 10.3). The second assessment was carried out 14 days after first assessment and Anolyte (AcquAttiva®) showed good efficacy to control disease (Chart 3, paragraph 10.3). Successively, all assessments have not showed any deasease symptoms. La valutazione dell’efficacia è stata effettata contando il numero di germogli infetti da ERWIAM. I germogli infetti sono stati rimossi da ogni plots. Alla prima valutazione Anolyte (AcquAttiva®) mostrava una buona efficacia nel controllo della malattia, ma la strategia Bio di riferimento ha mostrato un’efficacia più elevata (grafico 2, paragrafo 10.3). Alla seconda valutazione effettuata 14 giorni dopo la prima, Anolyte (AcquAttiva®) ha mostrato nuovamente una buona efficacia nel contenimento della malattia (grafico 3, paragrafo 10.3). Successivamente, in tutte le valutazioni non sis ono riscontrati sintomi legati a ERWIAM.

8.2 PHYTOTOXICITY All assessments have not showed any phytotoxicity symptoms. Tutte le valutazionI non hanno mostrato alcun sintomo di fitotossicità.




BIO pear

Pag. 14 di 38

Pagina 14 di 38



9 FINAL REMARKS

The experimental field trial was carried out in the countryside of Centallo (province of Cuneo –

Northern Italy) from May 2019 to July 2019, in order to evaluate the efficacy and selectivity of

Anolyte (AcquAttiva®) applied on BIO pear to control Erwinia amylovora (ERWIAM).

Anolyte (AcquAttiva®) showed good efficacy to control disease, but efficacy evaluation was not completely verified because the disease pressure was very low. All treatments did not show any phytotoxicity symptoms on the crop. La prova sperimentale è stata condotta a Centallo (provincia di Cuneo – Nord Italia) da Maggio 2019 a Luglio 2019, al fine di valutare l’efficacia e la selettività di Anolyte (AcquAttiva®) per il controllo di Erwinia amylovora (ERWIAM). Anolyte (AcquAttiva®) ha mostrato una buona efficacia nel controllo della malattia, ma la valutazione dell’efficacia non è stata completamente verificata in quanto la pressione della malattia era molto bassa. Tutti i trattamenti non hanno mostrato alcun sintomo di fitotossicità sulla coltura.




BIO pear

Pag. 15 di 38

Pagina 15 di 38



10 ANNEX

10.1 RAW DATA Pest Type D Disease D Disease D Disease D Disease Pest Code ERWIAM ERWIAM ERWIAM ERWIAM Pest Scientific Name Erwinia amylov> Erwinia amylov> Erwinia amylov> Erwinia amylov> Pest Name Fire blight Fire blight Fire blight Fire blight Crop Type, Code C PYUCO C PYUCO C PYUCO C PYUCO BBCH Scale BPOM BPOM BPOM BPOM Crop Scientific Name Pyrus communis Pyrus communis Pyrus communis Pyrus communis Crop Name Common pear Common pear Common pear Common pear Rating Date Mar-27-2019 Mar-27-2019 Apr-2-2019 Apr-2-2019 SE Group No. 22 1 23 2 Part Rated BUD P FLOCLU P BUD P FLOCLU P Rating Type COUINF COUINF COUINF COUINF Rating Unit NUMBER NUMBER NUMBER NUMBER Sample Size 1 PLOT 1 PLOT 1 PLOT 1 PLOT Number of Subsamples 1 1 1 1 Crop Stage Scale BBCH BBCH BBCH BBCH Crop Stage Majority 59 59 65 65 Crop Stage Minimum/Maximum 60 60 60 60 65 65 65 65 Assessed By I. Ramon I. Ramon I. Ramon I. Ramon Data Entry Date Sep-10-2019 Sep-10-2019 Sep-10-2019 Sep-10-2019 Days After First/Last Applic. 0 0 0 0 6 6 6 6

Trt Treatment Rate Appl No. Name Rate Unit Code Plot 1 2 3 4

1 Untreated Check 103 0,0 0,0 0,0 0,0 202 0,0 0,0 0,0 0,0 301 0,0 0,0 0,0 0,0 403 0,0 0,0 0,0 0,0

Mean = 0,0 0,0 0,0 0,0

2 Anolyte - AcquaAttiva 450 l/ha ABCDEFGH 101 0,0 0,0 0,0 0,0 203 0,0 0,0 0,0 0,0 302 0,0 0,0 0,0 0,0 401 0,0 0,0 0,0 0,0

Mean = 0,0 0,0 0,0 0,0

3 Amylo-X 2 kg/ha ABDF 102 0,0 0,0 0,0 0,0 Vacciplant 1 l/ha CE 201 0,0 0,0 0,0 0,0 Poltiglia Disperss 5 kg/ha GH 303 0,0 0,0 0,0 0,0

402 0,0 0,0 0,0 0,0 Mean = 0,0 0,0 0,0 0,0




BIO pear

Pag. 16 di 38

Pagina 16 di 38



Pest Type D Disease D Disease Pest Code ERWIAM ERWIAM Pest Scientific Name Erwinia amylov> Erwinia amylov> Pest Name Fire blight Fire blight Crop Type, Code C PYUCO C PYUCO C PYUCO C PYUCO BBCH Scale BPOM BPOM BPOM BPOM Crop Scientific Name Pyrus communis Pyrus communis Pyrus communis Pyrus communis Crop Name Common pear Common pear Common pear Common pear Rating Date Apr-2-2019 Apr-9-2019 Apr-9-2019 Apr-9-2019 SE Group No. 15 24 3 16 Part Rated PLANT C BUD P FLOCLU P PLANT C Rating Type PHYGEN COUINF COUINF PHYGEN Rating Unit % NUMBER NUMBER % Sample Size 1 PLOT 1 PLOT 1 PLOT 1 PLOT Number of Subsamples 1 1 1 1 Crop Stage Scale BBCH BBCH BBCH BBCH Crop Stage Majority 65 65 65 65 Crop Stage Minimum/Maximum 65 65 65 67 65 67 65 67 Assessed By I. Ramon I. Ramon I. Ramon I. Ramon Data Entry Date Sep-10-2019 Days After First/Last Applic. 6 6 13 7 13 7 13 7


1 Untreated Check 103 0,0 0,0 0,0 0,0 202 0,0 0,0 0,0 0,0 301 0,0 0,0 0,0 0,0 403 0,0 0,0 0,0 0,0

Mean = 0,0 0,0 0,0 0,0


Mean = 0,0 0,0 0,0 0,0 3 Amylo-X 2 kg/ha ABDF 102 0,0 0,0 0,0 0,0 Vacciplant 1 l/ha CE 201 0,0 0,0 0,0 0,0 Poltiglia Disperss 5 kg/ha GH 303 0,0 0,0 0,0 0,0

402 0,0 0,0 0,0 0,0 Mean = 0,0 0,0 0,0 0,0




BIO pear

Pag. 17 di 38

Pagina 17 di 38



Pest Type D Disease D Disease D Disease Pest Code ERWIAM ERWIAM ERWIAM Pest Scientific Name Erwinia amylov> Erwinia amylov> Erwinia amylov> Pest Name Fire blight Fire blight Fire blight Crop Type, Code C PYUCO C PYUCO C PYUCO C PYUCO BBCH Scale BPOM BPOM BPOM BPOM Crop Scientific Name Pyrus communis Pyrus communis Pyrus communis Pyrus communis Crop Name Common pear Common pear Common pear Common pear Rating Date Apr-17-2019 Apr-17-2019 Apr-17-2019 Apr-25-2019 SE Group No. 25 4 17 26 Part Rated BUD P FRUATT P PLANT C BUD P Rating Type COUINF COUINF PHYGEN COUINF Rating Unit NUMBER NUMBER % NUMBER Sample Size 1 PLOT 1 PLOT 1 PLOT 1 PLOT Number of Subsamples 1 1 1 1 Crop Stage Scale BBCH BBCH BBCH BBCH Crop Stage Majority 69 69 69 71 Crop Stage Minimum/Maximum 69 69 69 69 69 69 71 71 Assessed By I. Ramon I. Ramon I. Ramon I. Ramon Data Entry Date Sep-10-2019 Sep-10-2019 Days After First/Last Applic. 21 8 21 8 21 8 29 8


1 Untreated Check 103 0,0 0,0 0,0 0,0 202 0,0 0,0 0,0 0,0 301 0,0 0,0 0,0 0,0 403 0,0 0,0 0,0 0,0

Mean = 0,0 0,0 0,0 0,0



402 0,0 0,0 0,0 0,0 Mean = 0,0 0,0 0,0 0,0




BIO pear

Pag. 18 di 38

Pagina 18 di 38



Pest Type D Disease D Disease D Disease Pest Code ERWIAM ERWIAM ERWIAM Pest Scientific Name Erwinia amylov> Erwinia amylov> Erwinia amylov> Pest Name Fire blight Fire blight Fire blight Crop Type, Code C PYUCO C PYUCO C PYUCO C PYUCO BBCH Scale BPOM BPOM BPOM BPOM Crop Scientific Name Pyrus communis Pyrus communis Pyrus communis Pyrus communis Crop Name Common pear Common pear Common pear Common pear Rating Date Apr-25-2019 Apr-25-2019 May-3-2019 May-3-2019 SE Group No. 5 18 27 6 Part Rated FRUATT P PLANT C BUD P FRUATT P Rating Type COUINF PHYGEN COUINF COUINF Rating Unit NUMBER % NUMBER NUMBER Sample Size 1 PLOT 1 PLOT 1 PLOT 1 PLOT Number of Subsamples 1 1 1 1 Crop Stage Scale BBCH BBCH BBCH BBCH Crop Stage Majority 71 71 72 72 Crop Stage Minimum/Maximum 71 71 71 71 72 72 72 72 Assessed By I. Ramon I. Ramon I. Ramon I. Ramon Data Entry Date Sep-10-2019 Days After First/Last Applic. 29 8 29 8 37 8 37 8


1 Untreated Check 103 0,0 0,0 0,0 0,0 202 0,0 0,0 0,0 0,0 301 0,0 0,0 0,0 0,0 403 0,0 0,0 0,0 0,0

Mean = 0,0 0,0 0,0 0,0



402 0,0 0,0 0,0 0,0 Mean = 0,0 0,0 0,0 0,0




BIO pear

Pag. 19 di 38

Pagina 19 di 38



Pest Type D Disease D Disease Pest Code ERWIAM ERWIAM Pest Scientific Name Erwinia amylov> Erwinia amylov> Pest Name Fire blight Fire blight Crop Type, Code C PYUCO C PYUCO C PYUCO C PYUCO BBCH Scale BPOM BPOM BPOM BPOM Crop Scientific Name Pyrus communis Pyrus communis Pyrus communis Pyrus communis Crop Name Common pear Common pear Common pear Common pear Rating Date May-3-2019 May-22-2019 May-22-2019 May-22-2019 SE Group No. 19 28 7 20 Part Rated PLANT C BUD P FRUATT P FRUATT P Rating Type PHYGEN COUINF COUINF COUINF Rating Unit % NUMBER NUMBER NUMBER Sample Size 1 PLOT 1 PLOT 1 PLOT 1 PLOT Number of Subsamples 1 1 1 1 Crop Stage Scale BBCH BBCH BBCH BBCH Crop Stage Majority 72 73 73 73 Crop Stage Minimum/Maximum 72 72 73 73 73 73 73 73 Assessed By I. Ramon I. Ramon I. Ramon I. Ramon Data Entry Date Sep-10-2019 Sep-10-2019 Days After First/Last Applic. 37 8 56 19 56 19 56 19


1 Untreated Check 103 0,0 0,0 0,0 0,0 202 0,0 0,0 0,0 0,0 301 0,0 0,0 0,0 0,0 403 0,0 0,0 0,0 0,0

Mean = 0,0 0,0 0,0 0,0



402 0,0 0,0 0,0 0,0 Mean = 0,0 0,0 0,0 0,0




BIO pear

Pag. 20 di 38

Pagina 20 di 38



Pest Type D Disease D Disease D Disease Pest Code ERWIAM ERWIAM ERWIAM Pest Scientific Name Erwinia amylov> Erwinia amylov> Erwinia amylov> Pest Name Fire blight Fire blight Fire blight Crop Type, Code C PYUCO C PYUCO C PYUCO C PYUCO BBCH Scale BPOM BPOM BPOM BPOM Crop Scientific Name Pyrus communis Pyrus communis Pyrus communis Pyrus communis Crop Name Common pear Common pear Common pear Common pear Rating Date May-30-2019 May-30-2019 May-30-2019 Jun-7-2019 SE Group No. 29 8 21 30 Part Rated BUD P FRUATT P BUD P BUD P Rating Type COUINF COUINF COUINF COUINF Rating Unit NUMBER NUMBER NUMBER NUMBER Sample Size 1 PLOT 1 PLOT 1 PLOT 1 PLOT Number of Subsamples 1 1 1 1 Crop Stage Scale BBCH BBCH BBCH BBCH Crop Stage Majority 74 74 74 75 Crop Stage Minimum/Maximum 74 74 74 74 74 74 75 75 Assessed By I. Ramon I. Ramon I. Ramon I. Ramon Data Entry Date Sep-10-2019 Sep-10-2019 Sep-10-2019 Sep-10-2019 Days After First/Last Applic. 64 8 64 8 64 8 72 8


1 Untreated Check 103 20,0 0,0 0,0 0,0 202 6,0 0,0 0,0 0,0 301 10,0 0,0 0,0 0,0 403 13,0 0,0 0,0 0,0

Mean = 12,3 0,0 0,0 0,0



402 5,0 0,0 0,0 0,0 Mean = 5,0 0,0 0,0 0,0




BIO pear

Pag. 21 di 38

Pagina 21 di 38



Pest Type D Disease D Disease D Disease D Disease Pest Code ERWIAM ERWIAM ERWIAM ERWIAM Pest Scientific Name Erwinia amylov> Erwinia amylov> Erwinia amylov> Erwinia amylov> Pest Name Fire blight Fire blight Fire blight Fire blight Crop Type, Code C PYUCO C PYUCO C PYUCO C PYUCO BBCH Scale BPOM BPOM BPOM BPOM Crop Scientific Name Pyrus communis Pyrus communis Pyrus communis Pyrus communis Crop Name Common pear Common pear Common pear Common pear Rating Date Jun-7-2019 Jun-14-2019 Jun-14-2019 Jun-21-2019 SE Group No. 9 31 10 32 Part Rated FRUATT P BUD P FRUATT P BUD P Rating Type COUINF COUINF COUINF COUINF Rating Unit NUMBER NUMBER NUMBER NUMBER Sample Size 1 PLOT 1 PLOT 1 PLOT 1 PLOT Number of Subsamples 1 1 1 1 Crop Stage Scale BBCH BBCH BBCH BBCH Crop Stage Majority 75 76 76 77 Crop Stage Minimum/Maximum 75 75 76 76 76 76 77 77 Assessed By I. Ramon I. Ramon I. Ramon I. Ramon Data Entry Date Sep-10-2019 Nov-28-2019 Sep-10-2019 Sep-10-2019 Days After First/Last Applic. 72 8 79 15 79 15 86 22


1 Untreated Check 103 0,0 17,0 0,0 0,0 202 0,0 16,0 0,0 0,0 301 0,0 19,0 0,0 0,0 403 0,0 15,0 0,0 0,0

Mean = 0,0 16,8 0,0 0,0



402 0,0 6,0 0,0 0,0 Mean = 0,0 4,3 0,0 0,0




BIO pear

Pag. 22 di 38

Pagina 22 di 38



Pest Type D Disease D Disease D Disease D Disease Pest Code ERWIAM ERWIAM ERWIAM ERWIAM Pest Scientific Name Erwinia amylov> Erwinia amylov> Erwinia amylov> Erwinia amylov> Pest Name Fire blight Fire blight Fire blight Fire blight Crop Type, Code C PYUCO C PYUCO C PYUCO C PYUCO BBCH Scale BPOM BPOM BPOM BPOM Crop Scientific Name Pyrus communis Pyrus communis Pyrus communis Pyrus communis Crop Name Common pear Common pear Common pear Common pear Rating Date Jun-21-2019 Jun-28-2019 Jun-28-2019 Jul-12-2019 SE Group No. 11 33 12 34 Part Rated FRUATT P BUD P FRUATT P BUD P Rating Type COUINF COUINF COUINF COUINF Rating Unit NUMBER NUMBER NUMBER NUMBER Sample Size 1 PLOT 1 PLOT 1 PLOT 1 PLOT Number of Subsamples 1 1 1 1 Crop Stage Scale BBCH BBCH BBCH BBCH Crop Stage Majority 77 78 78 78 Crop Stage Minimum/Maximum 77 77 78 78 78 78 78 78 Assessed By I. Ramon I. Ramon I. Ramon I. Ramon Data Entry Date Sep-10-2019 Sep-10-2019 Days After First/Last Applic. 86 22 93 29 93 29 107 43


1 Untreated Check 103 0,0 0,0 0,0 0,0 202 0,0 0,0 0,0 0,0 301 0,0 0,0 0,0 0,0 403 0,0 0,0 0,0 0,0

Mean = 0,0 0,0 0,0 0,0



402 0,0 0,0 0,0 0,0 Mean = 0,0 0,0 0,0 0,0




BIO pear

Pag. 23 di 38

Pagina 23 di 38



Pest Type D Disease D Disease D Disease Pest Code ERWIAM ERWIAM ERWIAM Pest Scientific Name Erwinia amylov> Erwinia amylov> Erwinia amylov> Pest Name Fire blight Fire blight Fire blight Crop Type, Code C PYUCO C PYUCO C PYUCO BBCH Scale BPOM BPOM BPOM Crop Scientific Name Pyrus communis Pyrus communis Pyrus communis Crop Name Common pear Common pear Common pear Rating Date Jul-12-2019 Jul-26-2019 Jul-26-2019 SE Group No. 13 35 14 Part Rated FRUATT P BUD P BUD P Rating Type COUINF COUINF COUINF Rating Unit NUMBER NUMBER NUMBER Sample Size 1 PLOT 1 PLOT 1 PLOT Number of Subsamples 1 1 1 Crop Stage Scale BBCH BBCH BBCH Crop Stage Majority 78 79 79 Crop Stage Minimum/Maximum 78 78 79 79 79 79 Assessed By I. Ramon I. Ramon I. Ramon Data Entry Date Sep-10-2019 Sep-10-2019 Days After First/Last Applic. 107 43 121 57 121 57

Trt Treatment Rate Appl No. Name Rate Unit Code Plot 33 34 35

1 Untreated Check 103 0,0 0,0 0,0 202 0,0 0,0 0,0 301 0,0 0,0 0,0 403 0,0 0,0 0,0

Mean = 0,0 0,0 0,0

2 Anolyte - AcquaAttiva 450 l/ha ABCDEFGH 101 0,0 0,0 0,0 203 0,0 0,0 0,0 302 0,0 0,0 0,0 401 0,0 0,0 0,0

Mean = 0,0 0,0 0,0 3 Amylo-X 2 kg/ha ABDF 102 0,0 0,0 0,0 Vacciplant 1 l/ha CE 201 0,0 0,0 0,0 Poltiglia Disperss 5 kg/ha GH 303 0,0 0,0 0,0

402 0,0 0,0 0,0 Mean = 0,0 0,0 0,0




BIO pear

Pag. 24 di 38

Pagina 24 di 38



Pest Type D, Disease = Disease, such as a fungus, bacteria, or virus Pest Code ERWIAM, Erwinia amylovora, Fire blight = US Crop Type, Code C = EPPO species (Bayer) codes PYUCO, BPOM, Pyrus communis, Common pear = US Part Rated BUD = bud FLOCLU = flower cluster PLANT = plant FRUATT = fruit - attached P = Pest is Part Rated C = Crop is Part Rated Rating Type COUINF = count - infected PHYGEN = phytotoxicity - general / injury Rating Unit NUMBER = number % = percent PLOT = total plot Crop Stage Scale BBCH = BBCH uniform plant stages Crop Stage Majority 59 = Most flowers with petals forming a hollow ball 65 = Full flowerering: at least 50% of flowers open 69 = End of flowering: all petals fallen 71 = Fruit size up to 10mm; fruit fall after flowering 72 = Fruit size up to 20 mm 73 = Second fruit fall 74 = Fruit diameter up to 40 mm; fruit erect (T-stage: underside of fruit and stalk) 75 = Fruit about half final size 76 = Fruit about 60% of final size 77 = Fruit about 70% of final size 78 = Fruit about 80% of final size 79 = Fruit about 90% of final size Crop Stage Minimum/Maximum 60 = First flowers open 65 = Full flowerering: at least 50% of flowers open 69 = End of flowering: all petals fallen 71 = Fruit size up to 10mm; fruit fall after flowering 72 = Fruit size up to 20 mm 73 = Second fruit fall 74 = Fruit diameter up to 40 mm; fruit erect (T-stage: underside of fruit and stalk) 75 = Fruit about half final size 76 = Fruit about 60% of final size 77 = Fruit about 70% of final size 78 = Fruit about 80% of final size 79 = Fruit about 90% of final size 67 = Flowers fading: majority of petals fallen




BIO pear

Pag. 25 di 38

Pagina 25 di 38



10.2 RAW WEATHER DATA

Closest Weather Station: Centallo Distance: 5 km

Moisture Min Max Avg Temp % Relative No. Date Total Unit Temp Temp Temp Unit Humidity

1. May-1-2019 0 mm 7,8 21,4 14,2 C 49

2. May-2-2019 2 mm 7,8 19,6 13,6 C 69 3. May-3-2019 8,4 mm 9,5 18,1 12,7 C 81

4. May-4-2019 3,4 mm 8,4 20,3 13,3 C 67

5. May-5-2019 3,8 mm 4,2 14,6 9,9 C 52

6. May-6-2019 0 mm 3,2 17,3 10,4 C 43 7. May-7-2019 0 mm 3,4 17,7 10,8 C 51

8. May-8-2019 6,2 mm 6,9 12,5 10 C 84

9. May-9-2019 0,2 mm 6,9 19,7 13 C 71

10. May-10-2019 0 mm 6,7 22,5 14,6 C 62 11. May-11-2019 2,2 mm 9,9 22,2 15,1 C 67

12. May-12-2019 0 mm 7,2 19,9 14,2 C 36

13. May-13-2019 0 mm 9,1 20,1 14,3 C 38

14. May-14-2019 0 mm 7,1 21,4 14,3 C 45 15. May-15-2019 0,4 mm 6,2 17,6 12,1 C 42

16. May-16-2019 0 mm 4 18,4 12 C 56

17. May-17-2019 11 mm 8,8 13,5 11,2 C 83

18. May-18-2019 24,4 mm 8,6 11,5 9,8 C 97 19. May-19-2019 6,2 mm 9,6 15,3 11,6 C 89

20. May-20-2019 0,2 mm 8 20,9 14 C 73

21. May-21-2019 0 mm 7,5 22,6 15,1 C 68

22. May-22-2019 6,6 mm 11,8 23,5 17,2 C 63 23. May-23-2019 0 mm 10,4 25,2 17,6 C 62

24. May-24-2019 0 mm 11,1 24,4 17 C 66

25. May-25-2019 41,6 mm 10,9 24,2 15,9 C 73

26. May-26-2019 31,8 mm 12,4 20 15 C 82 27. May-27-2019 6,2 mm 13,4 17,9 14,8 C 86

28. May-28-2019 3,6 mm 10,9 20,2 15 C 79

29. May-29-2019 0,2 mm 8,3 23 16,4 C 42

30. May-30-2019 0 mm 10,3 23,8 17,6 C 40 31. May-31-2019 0 mm 11,3 23,5 16,9 C 59

32. Jun-1-2019 0 mm 12 26,9 19,2 C 54

33. Jun-2-2019 0 mm 13,4 28,6 21,2 C 52

34. Jun-3-2019 0 mm 15,8 29,7 21,7 C 55 35. Jun-4-2019 0 mm 14,1 29,6 21,7 C 56

36. Jun-5-2019 0 mm 15,7 28,1 21,5 C 60

37. Jun-6-2019 0 mm 12,9 24,3 18,5 C 66 38. Jun-7-2019 0 mm 13,9 23,3 17,9 C 69

39. Jun-8-2019 0 mm 13,1 27,5 20,2 C 58

40. Jun-9-2019 1,6 mm 15,4 24,5 19,7 C 66

41. Jun-10-2019 0,8 mm 14,7 23,8 19,4 C 69 42. Jun-11-2019 0 mm 13 23,8 18,5 C 59

43. Jun-12-2019 0 mm 13 26,8 19,6 C 57

44. Jun-13-2019 0 mm 10,8 26,4 19,1 C 52

45. Jun-14-2019 0,6 mm 14,7 28,3 21,1 C 47 46. Jun-15-2019 0 mm 14,7 28,5 21,1 C 54

47. Jun-16-2019 0 mm 12,7 30,1 21,6 C 45

48. Jun-17-2019 0 mm 14,8 29,3 22,3 C 45

49. Jun-18-2019 0 mm 16,5 30,5 22,3 C 52 50. Jun-19-2019 0 mm 16 31,3 23,1 C 55

51. Jun-20-2019 0 mm 16 29,3 22,2 C 61

52. Jun-21-2019 0 mm 14,9 28,7 20,7 C 69

53. Jun-22-2019 13,4 mm 14,8 25,7 18,7 C 71 54. Jun-23-2019 0,2 mm 13,1 29 21,5 C 60




BIO pear

Pag. 26 di 38

Pagina 26 di 38



55. Jun-24-2019 0 mm 17,3 32 24,7 C 65

56. Jun-25-2019 0 mm 21 33,1 26,6 C 67

57. Jun-26-2019 0 mm 20,6 34,4 27,5 C 72 58. Jun-27-2019 0 mm 21,2 38,3 29,2 C 64

59. Jun-28-2019 0 mm 21,6 37,9 29,2 C 50

60. Jun-29-2019 1,8 mm 22,4 31,5 26,1 C 58

61. Jun-30-2019 0 mm 20,9 32,7 26 C 62 62. Jul-1-2019 0,8 mm 19,2 34,5 25,7 C 52

63. Jul-2-2019 0 mm 16,9 32,8 24,5 C 58

64. Jul-3-2019 0,4 mm 15,9 31,4 23,4 C 50

65. Jul-4-2019 0 mm 16,6 30,8 23,8 C 57 66. Jul-5-2019 0 mm 17,9 33,6 25,6 C 65

67. Jul-6-2019 0 mm 18,2 33,1 25,5 C 63

68. Jul-7-2019 0 mm 19 35,9 25,6 C 69 69. Jul-8-2019 0 mm 15,3 33,6 23,7 C 54

70. Jul-9-2019 0,4 mm 14,5 25,2 20,1 C 78

71. Jul-10-2019 16,2 mm 14,8 26,1 20 C 85

72. Jul-11-2019 0 mm 15,1 24,3 19,6 C 86 73. Jul-12-2019 0 mm 14,2 30,6 21,7 C 69

74. Jul-13-2019 0 mm 11,2 31 21,5 C 60

75. Jul-14-2019 3 mm 14,8 28,1 20,3 C 69

76. Jul-15-2019 76,6 mm 9,8 16 12,5 C 95 77. Jul-16-2019 2,6 mm 10 28,3 18,7 C 71

78. Jul-17-2019 0 mm 11,8 29,3 20 C 73

79. Jul-18-2019 0 mm 13,5 28,5 20,4 C 75

80. Jul-19-2019 0 mm 14,7 29,4 21,3 C 75 81. Jul-20-2019 0 mm 16,8 30,2 22,6 C 73

82. Jul-21-2019 0 mm 15,9 31,2 22,8 C 79

83. Jul-22-2019 0 mm 16,9 32,1 23,8 C 77

84. Jul-23-2019 0 mm 17,5 33,2 24,2 C 77 85. Jul-24-2019 0 mm 17,1 34,2 24,6 C 77

86. Jul-25-2019 0 mm 18,4 35,5 26,2 C 70

87. Jul-26-2019 0 mm 19,2 34,5 26,1 C 71

88. Jul-27-2019 29 mm 12,7 28,1 19 C 92 89. Jul-28-2019 15,8 mm 12,4 29,6 20,3 C 83

90. Jul-29-2019 0 mm 14 31 22,1 C 79

91. Jul-30-2019 0 mm 15,5 32,1 23,3 C 78

92. Jul-31-2019 0 mm 14,9 30,8 22,5 C 79




BIO pear

Pag. 27 di 38

Pagina 27 di 38



10.3 CHARTS

Chart 2 - Number of deseased bud at first assessment (30/05/2019)

Chart 3 - Number of deseased bud at second assessement (14/07/2019)




BIO pear

Pag. 28 di 38

Pagina 28 di 38



10.4 STATISTICAL ANALYSIS Pest Type D Disease D Disease D Disease D Disease

Pest Code ERWIAM ERWIAM ERWIAM ERWIAM

Pest Scientific Name Erwinia amylov> Erwinia amylov> Erwinia amylov> Erwinia amylov>

Pest Name Fire blight Fire blight Fire blight Fire blight

Crop Type, Code C PYUCO C PYUCO C PYUCO C PYUCO C PYUCO

BBCH Scale BPOM BPOM BPOM BPOM BPOM

Crop Scientific Name Pyrus communis Pyrus communis Pyrus communis Pyrus communis Pyrus communis

Crop Name Common pear Common pear Common pear Common pear Common pear

Rating Date Mar-27-2019 Mar-27-2019 Apr-2-2019 Apr-2-2019 Apr-2-2019

SE Group No. 22 1 23 2 15

Part Rated BUD P FLOCLU P BUD P FLOCLU P PLANT C

Rating Type COUINF COUINF COUINF COUINF PHYGEN

Rating Unit NUMBER NUMBER NUMBER NUMBER %

Sample Size 1 PLOT 1 PLOT 1 PLOT 1 PLOT 1 PLOT

Number of Subsamples 1 1 1 1 1

Crop Stage Scale BBCH BBCH BBCH BBCH BBCH

Crop Stage Majority 59 59 65 65 65

Crop Stage Minimum/Maximum 60 60 60 60 65 65 65 65 65 65

Assessed By I. Ramon I. Ramon I. Ramon I. Ramon I. Ramon

Data Entry Date Sep-10-2019 Sep-10-2019 Sep-10-2019 Sep-10-2019

Days After First/Last Applic. 0 0 0 0 6 6 6 6 6 6

Trt Treatment Rate Appl No. Name Rate Unit Code 1* 2* 3* 4* 5*

1 Untreated Check 0,0 - 0,0 - 0,0 - 0,0 - 0,0 -

0,0 StDev 0,0 StDev 0,0 StDev 0,0 StDev 0,0 StDev

2 Anolyte - AcquaAttiva

450 l/ha ABCDEFGH 0,0 - 0,0 - 0,0 - 0,0 - 0,0 -


3 Amylo-X 2 kg/ha ABDF 0,0 - 0,0 - 0,0 - 0,0 - 0,0 -

Vacciplant 1 l/ha CE 0,0 StDev 0,0 StDev 0,0 StDev 0,0 StDev 0,0 StDev

Poltiglia Disperss 5 kg/ha GH

Tukey's HSD P=.05 . . . . .

Standard Deviation 0,00 0,00 0,00 0,00 0,00

CV 0,0 0,0 0,0 0,0 0,0

Levene's F 0,00 0,00 0,00 0,00 0,00

Levene's Prob(F) . . . . .

Skewness . . . . .

Kurtosis . . . . .

Replicate F 0,000 0,000 0,000 0,000 0,000

Replicate Prob(F) 1,0000 1,0000 1,0000 1,0000 1,0000

Treatment F 0,000 0,000 0,000 0,000 0,000

Treatment Prob(F) 1,0000 1,0000 1,0000 1,0000 1,0000




BIO pear

Pag. 29 di 38

Pagina 29 di 38



Pest Type D Disease D Disease D Disease D Disease








Rating Date Apr-9-2019 Apr-9-2019 Apr-9-2019 Apr-17-2019 Apr-17-2019

SE Group No. 24 3 16 25 4

Part Rated BUD P FLOCLU P PLANT C BUD P FRUATT P

Rating Type COUINF COUINF PHYGEN COUINF COUINF

Rating Unit NUMBER NUMBER % NUMBER NUMBER







Data Entry Date Sep-10-2019 Sep-10-2019


Trt Treatment Rate Appl

No. Name Rate Unit Code 6* 7* 8* 9* 10*

1 Untreated Check 0,0 - 0,0 - 0,0 - 0,0 - 0,0 -



450 l/ha ABCDEFGH 0,0 - 0,0 - 0,0 - 0,0 - 0,0 -


3 Amylo-X 2 kg/ha ABDF 0,0 - 0,0 - 0,0 - 0,0 - 0,0 -



Tukey's HSD P=.05 . . . . .


CV 0,0 0,0 0,0 0,0 0,0

Levene's F 0,00 0,00 0,00 0,00 0,00


Skewness . . . . .

Kurtosis . . . . .

Replicate F 0,000 0,000 0,000 0,000 0,000

Replicate Prob(F) 1,0000 1,0000 1,0000 1,0000 1,0000

Treatment F 0,000 0,000 0,000 0,000 0,000

Treatment Prob(F) 1,0000 1,0000 1,0000 1,0000 1,0000




BIO pear

Pag. 30 di 38

Pagina 30 di 38



Pest Type D Disease D Disease D Disease

Pest Code ERWIAM ERWIAM ERWIAM

Pest Scientific Name Erwinia amylov> Erwinia amylov> Erwinia amylov>

Pest Name Fire blight Fire blight Fire blight





Rating Date Apr-17-2019 Apr-25-2019 Apr-25-2019 Apr-25-2019 May-3-2019

SE Group No. 17 26 5 18 27

Part Rated PLANT C BUD P FRUATT P PLANT C BUD P

Rating Type PHYGEN COUINF COUINF PHYGEN COUINF

Rating Unit % NUMBER NUMBER % NUMBER











1 Untreated Check 0,0 - 0,0 - 0,0 - 0,0 - 0,0 -



450 l/ha ABCDEFGH 0,0 - 0,0 - 0,0 - 0,0 - 0,0 -


3 Amylo-X 2 kg/ha ABDF 0,0 - 0,0 - 0,0 - 0,0 - 0,0 -



Tukey's HSD P=.05 . . . . .


CV 0,0 0,0 0,0 0,0 0,0

Levene's F 0,00 0,00 0,00 0,00 0,00


Skewness . . . . .

Kurtosis . . . . .

Replicate F 0,000 0,000 0,000 0,000 0,000

Replicate Prob(F) 1,0000 1,0000 1,0000 1,0000 1,0000

Treatment F 0,000 0,000 0,000 0,000 0,000

Treatment Prob(F) 1,0000 1,0000 1,0000 1,0000 1,0000




BIO pear

Pag. 31 di 38

Pagina 31 di 38



Pest Type D Disease D Disease D Disease

Pest Code ERWIAM ERWIAM ERWIAM

Pest Scientific Name Erwinia amylov> Erwinia amylov> Erwinia amylov>

Pest Name Fire blight Fire blight Fire blight





Rating Date May-3-2019 May-3-2019 May-22-2019 May-22-2019 May-22-2019

SE Group No. 6 19 28 7 20

Part Rated FRUATT P PLANT C BUD P FRUATT P FRUATT P

Rating Type COUINF PHYGEN COUINF COUINF COUINF

Rating Unit NUMBER % NUMBER NUMBER NUMBER











1 Untreated Check 0,0 - 0,0 - 0,0 - 0,0 - 0,0 -



450 l/ha ABCDEFGH 0,0 - 0,0 - 0,0 - 0,0 - 0,0 -


3 Amylo-X 2 kg/ha ABDF 0,0 - 0,0 - 0,0 - 0,0 - 0,0 -



Tukey's HSD P=.05 . . . . .


CV 0,0 0,0 0,0 0,0 0,0

Levene's F 0,00 0,00 0,00 0,00 0,00


Skewness . . . . .

Kurtosis . . . . .

Replicate F 0,000 0,000 0,000 0,000 0,000

Replicate Prob(F) 1,0000 1,0000 1,0000 1,0000 1,0000

Treatment F 0,000 0,000 0,000 0,000 0,000

Treatment Prob(F) 1,0000 1,0000 1,0000 1,0000 1,0000




BIO pear

Pag. 32 di 38

Pagina 32 di 38



Pest Type D Disease D Disease D Disease D Disease








Rating Date May-30-2019 May-30-2019 May-30-2019 Jun-7-2019 Jun-7-2019

SE Group No. 29 8 21 30 9

Part Rated BUD P FRUATT P BUD P BUD P FRUATT P

Rating Type COUINF COUINF COUINF COUINF COUINF

Rating Unit NUMBER NUMBER NUMBER NUMBER NUMBER







Data Entry Date Sep-10-2019 Sep-10-2019 Sep-10-2019 Sep-10-2019 Sep-10-2019




1 Untreated Check 12,3 a 0,0 - 0,0 - 0,0 - 0,0 -



450 l/ha ABCDEFGH 6,5 b 0,0 - 0,0 - 0,0 - 0,0 -


3 Amylo-X 2 kg/ha ABDF 5,0 b 0,0 - 0,0 - 0,0 - 0,0 -



Tukey's HSD P=.05 5,70 . . . .


CV 33,22 0,0 0,0 0,0 0,0

Levene's F 1,901 0,00 0,00 0,00 0,00

Levene's Prob(F) 0,205 . . . .

Skewness 1,5577* . . . .

Kurtosis 3,0128* . . . .

Replicate F 4,542 0,000 0,000 0,000 0,000

Replicate Prob(F) 0,0548 1,0000 1,0000 1,0000 1,0000

Treatment F 8,470 0,000 0,000 0,000 0,000

Treatment Prob(F) 0,0179 1,0000 1,0000 1,0000 1,0000




BIO pear

Pag. 33 di 38

Pagina 33 di 38



Pest Type D Disease D Disease D Disease D Disease D Disease

Pest Code ERWIAM ERWIAM ERWIAM ERWIAM ERWIAM

Pest Scientific Name Erwinia amylov> Erwinia amylov> Erwinia amylov> Erwinia amylov> Erwinia amylov>

Pest Name Fire blight Fire blight Fire blight Fire blight Fire blight





Rating Date Jun-14-2019 Jun-14-2019 Jun-21-2019 Jun-21-2019 Jun-28-2019

SE Group No. 31 10 32 11 33

Part Rated BUD P FRUATT P BUD P FRUATT P BUD P









Data Entry Date Nov-28-2019 Sep-10-2019 Sep-10-2019 Sep-10-2019




1 Untreated Check 16,8 a 0,0 - 0,0 - 0,0 - 0,0 -



450 l/ha ABCDEFGH 11,0 b 0,0 - 0,0 - 0,0 - 0,0 -


3 Amylo-X 2 kg/ha ABDF 4,3 c 0,0 - 0,0 - 0,0 - 0,0 -



Tukey's HSD P=.05 4,47 . . . .


CV 19,33 0,0 0,0 0,0 0,0

Levene's F 0,043 0,00 0,00 0,00 0,00

Levene's Prob(F) 0,958 . . . .

Skewness -0,1834 . . . .

Kurtosis -1,0001 . . . .

Replicate F 0,471 0,000 0,000 0,000 0,000

Replicate Prob(F) 0,7138 1,0000 1,0000 1,0000 1,0000

Treatment F 36,843 0,000 0,000 0,000 0,000

Treatment Prob(F) 0,0004 1,0000 1,0000 1,0000 1,0000




BIO pear

Pag. 34 di 38

Pagina 34 di 38



Pest Type D Disease D Disease D Disease D Disease D Disease

Pest Code ERWIAM ERWIAM ERWIAM ERWIAM ERWIAM

Pest Scientific Name Erwinia amylov> Erwinia amylov> Erwinia amylov> Erwinia amylov> Erwinia amylov>

Pest Name Fire blight Fire blight Fire blight Fire blight Fire blight





Rating Date Jun-28-2019 Jul-12-2019 Jul-12-2019 Jul-26-2019 Jul-26-2019

SE Group No. 12 34 13 35 14

Part Rated FRUATT P BUD P FRUATT P BUD P BUD P









Data Entry Date Sep-10-2019 Sep-10-2019 Sep-10-2019




1 Untreated Check 0,0 - 0,0 - 0,0 - 0,0 - 0,0 -



450 l/ha ABCDEFGH 0,0 - 0,0 - 0,0 - 0,0 - 0,0 -


3 Amylo-X 2 kg/ha ABDF 0,0 - 0,0 - 0,0 - 0,0 - 0,0 -



Tukey's HSD P=.05 . . . . .


CV 0,0 0,0 0,0 0,0 0,0

Levene's F 0,00 0,00 0,00 0,00 0,00


Skewness . . . . .

Kurtosis . . . . .

Replicate F 0,000 0,000 0,000 0,000 0,000

Replicate Prob(F) 1,0000 1,0000 1,0000 1,0000 1,0000

Treatment F 0,000 0,000 0,000 0,000 0,000

Treatment Prob(F) 1,0000 1,0000 1,0000 1,0000 1,0000




BIO pear

Pag. 35 di 38

Pagina 35 di 38



Pest Type D, Disease = Disease, such as a fungus, bacteria, or virus Pest Code ERWIAM, Erwinia amylovora, Fire blight = US Crop Type, Code C = EPPO species (Bayer) codes PYUCO, BPOM, Pyrus communis, Common pear = US Part Rated BUD = bud FLOCLU = flower cluster PLANT = plant FRUATT = fruit - attached P = Pest is Part Rated C = Crop is Part Rated Rating Type COUINF = count - infected PHYGEN = phytotoxicity - general / injury Rating Unit NUMBER = number % = percent PLOT = total plot Crop Stage Scale BBCH = BBCH uniform plant stages Crop Stage Majority 59 = Most flowers with petals forming a hollow ball 65 = Full flowerering: at least 50% of flowers open 69 = End of flowering: all petals fallen 71 = Fruit size up to 10mm; fruit fall after flowering 72 = Fruit size up to 20 mm 73 = Second fruit fall 74 = Fruit diameter up to 40 mm; fruit erect (T-stage: underside of fruit and stalk) 75 = Fruit about half final size 76 = Fruit about 60% of final size 77 = Fruit about 70% of final size 78 = Fruit about 80% of final size 79 = Fruit about 90% of final size Crop Stage Minimum/Maximum 60 = First flowers open 65 = Full flowerering: at least 50% of flowers open 69 = End of flowering: all petals fallen 71 = Fruit size up to 10mm; fruit fall after flowering 72 = Fruit size up to 20 mm 73 = Second fruit fall 74 = Fruit diameter up to 40 mm; fruit erect (T-stage: underside of fruit and stalk) 75 = Fruit about half final size 76 = Fruit about 60% of final size 77 = Fruit about 70% of final size 78 = Fruit about 80% of final size 79 = Fruit about 90% of final size 67 = Flowers fading: majority of petals fallen




BIO pear

Pag. 36 di 38

Pagina 36 di 38



10.5 TRIAL SITE LOCATION




BIO pear

Pag. 37 di 38

Pagina 37 di 38






BIO pear

Pag. 38 di 38

Pagina 38 di 38



Documents

FIELD TRIAL REPORT · FIELD TRIAL REPORT Efficacy and selectivity of Anolyte (AcquAttiva®) to control Erwinia amylovora causing fireblight on BIO pear PROTOCOL ID:1062018G_B