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Figure 14-7 (part 1 of 2) Molecular Biology of the Cell (© Garland Science 2008)
Biochemical fractionation of purified mitochondria into separate components
Discussion (III) from Dr. Jen
Figure 14-7 (part 2 of 2) Molecular Biology of the Cell (© Garland Science 2008)
Figure 14-1 Molecular Biology of the Cell (© Garland Science 2008)
Harnessing energy for life—Chemiosmotic coupling
NADH/FADH2化學能 H+電位能 ATP化學能
Q1:
How can you proof that there are membrane-requiring steps (chemiosmotic coupling processes) in the energy transfer between high energy electrons and ATP synthesis in the mitochondria?
(Maximal 5 slides including the title page)
Figure 14-32 Molecular Biology of the Cell (© Garland Science 2008)
Critical roles of mitochondria in cell metabolism (besides ATP production).
Note: Incomplete oxidative phosphorylation (0.05%) often leads to the formation of reactive oxygen species (ROS). About 90% of the cell’s O2
- is formed inside mitochondria.
Collapse of mitochondrial Δ eventually leads to cell death.
Q2:
It is well-known that the collapse of mitochondria membrane potential usually leads to cell death. Based on what your have learned from this chapter, give at least 3 reasons to explain this fact and speculate how would the “dying process” proceed?
(Maximal 5 slides including the title page)
Figure 14-56 Molecular Biology of the Cell (© Garland Science 2008)
(Mfn 1, Mfn 2, Opa 1)
(Drp 1, Fis 1)
Q3: What are the advantages for mitochondrial fusion/fission?
Figure 14-62 Molecular Biology of the Cell (© Garland Science 2008)
The differences in the patterns of inheritance between mitochondrial and nuclear genes of yeast cells.
Mitotic segregation, non-Mendelian or cytoplasmic inheritance
Chloramphenicol:A procaryotic protein synthesis inhibitor
Table 14-3 Molecular Biology of the Cell (© Garland Science 2008)
Q4: Mitochondria in various organisms use a relatively relaxed codon system. Moreover, mitochondrial genes are inherited by a non-Mendelian mechanism. What could have caused these phenomena, and would mitochondria in different organisms behave differently?
(Maximal 5 slides including the title page)
Figure 14-59 Molecular Biology of the Cell (© Garland Science 2008)
Comparison of mitochondrial genomes.
Q5: What are the consequences (advantages and disadvantages) of having a large or a small genome in the mitochondria?
(Maximal 5 slides including the title page)