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Figure S1: Mathematical model of CD14 signaling pathway
Figure S2: Mathematical model of PI3K signaling pathway
Figure S3: Mathematical model of TNF signaling pathway
Figure S4: Mathematical model of EGF signaling pathway
Figure S5: Mathematical model of MAPK signaling networkReaction Species Compartment
Figure S6: Simulation graphs of (A) CD14 (B) TLR4
(A) (B)
Figure S7: Simulation graphs of (A) cytoplasmic NF-kappa B (B) EGF (C) TNF
(A) (B) (C)
Figure S8: Simulation graph of entire MAPK network
(A) (B) (C)
Figure S9: Simulation graphs of TRAF-6 crosstalk point in (A) TNF (B) CD14 (C) MAPK network
(A) (B)
(C) (D)Figure S10: Simulation graphs of MKK3/6 crosstalk point in (A) TNF (B) CD14 (C) EGF (D) MAPK network
(A) (B)
(C) (D)Figure S11: Simulation graphs of MKK4/7 crosstalk point in (A) TNF (B) CD14 (C) EGF (D) MAPK network
(A) (B) (C)
Figure S12: Simulation graphs of PIP2 crosstalk point in (A) EGF (B) PI3K (C) MAPK network
Figure S13: Sensitivity plot of CD14
Figure S14: Sensitivity plot of TLR4
Figure S15: Sensitivity plot of IRAK4/1
Figure S16: Sensitivity plot of EGF
Figure S17: Sensitivity plot of TNF
Figure S18: Sensitivity plot of TRAF-6
Figure S19: Sensitivity plot of cytoplasmic NF-kappa B
Pathways Mean path length Diameter Average
paths
EGF 7 13 26
TNF 5 9 22.6667
CD14 5 9 14.333
PI3K 7 13 10.923
Table S1: Mean path length, diameter and average paths for individual pathways
Clustering coefficient 0.017
Diameter 12
Radius 1
Average paths 10.31
Mean path length 4.902
Number of nodes 72
Number of edges 88
Isolated nodes 0
Self loops 0
Table S2: Statistics of the MAPK network
Reactions Flux
cytoplasm.CD14 → cytoplasm.TLR4 0.485643
cytoplasm.TLR4 → cytoplasm.MyD88 0.211183
cytoplasm.[IRAK4/1] → cytoplasm.[TRAF-6] 0.201431
cytoplasm.TNF → cytoplasm.TNFR 0.369697
cytoplasm.[TRAF-6] → cytoplasm.ECSIT 0.233653
cytoplasm.TAK1 → cytoplasm.[MKK4/7] 0.294193
cytoplasm.ASK1 → cytoplasm.[MKK3/6] 0.265605
cytoplasm.[IkB/NF-kappa B] → cytoplasm.[NF-kappa B] 0.337536
cytoplasm.EGF → cytoplasm.EGF_EGFR 0.491873
cytoplasm.EGF_EGFR → cytoplasm.EGF_EGFR_EGF_EGFR 0.229942
cytoplasm.MEK → cytoplasm.MEKp 0.223247
nucleus.[ERK1/2] → nucleus.Elk 0.216036
Table S3: Reactions with high flux values in the network
Reactions Sensitivity
cytoplasm.CD14 → cytoplasm.TLR4 0.499
cytoplasm.TLR4 → cytoplasm.MyD88 0.581
cytoplasm.[IRAK4/1] → cytoplasm.[TRAF-6] 0.399
cytoplasm.TNF → cytoplasm.TNFR 0.524
cytoplasm.[TRAF-6] → cytoplasm.ECSIT 0.614
cytoplasm.TAK1 → cytoplasm.[MKK4/7] 0.487
cytoplasm.ASK1 → cytoplasm.[MKK3/6] 0.796
cytoplasm.[IkB/NF-kappa B] → cytoplasm.[NF-kappa B] 0.714
cytoplasm.[NF-kappa B] → nucleus.[NF-kappa B] 0.593
cytoplasm.EGF → cytoplasm.EGF_EGFR 0.601
cytoplasm.EGF_EGFR → cytoplasm.EGF_EGFR_EGF_EGFR 0.757
cytoplasm.MEK → cytoplasm.MEKp 0.349
nucleus.[ERK1/2] → nucleus.Elk 0.593
Table S4: Reactions having high sensitivity coefficient (Wi,j) valuesin the network