Unique bio-mimicking "Fish-tail" bioagitator:- highly efficient mixing without eddies- free of cutting edges- produces no shear stress

'rISB-TAIL' AGITATOR

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Did you know, it took over 500 millionsyears for nature to develop a perfect fishtail for energy transmission in the water?

Only LAMBDA uses this know-how of nature toproduce a unique Bio-mimicking "Fish-tail"bioagitator...

Move it like a fish!The agitating discs of the 'fish-tail' bioagitator are elasticand have the typical form of fish tails.

In analogy to a fish tail, this shape of the stirring discproduces an efficient energy transfer in liquid (culturemedium).

Have you ever seen a fish moving with a propellerinstead of a tail?

Bioagitator - An optimal choice for cell culture

d Gentle and efficient mixing in both horizontal and~~ vertical direction. ~ f \ ~ ~

~ No cutting ~dges and. no. eddies or turbulence ~formed dunng the agitation. '0_~

d Efficient medium circulation with the up and down ~ ~~ movement of the fish-tail discs. C_~

d Cells are not broken or destroyed due to the c::::~ /}~ hydrodynamic shear stress. ~ _

~OPtima l oxygenation and gas exchange in the ~~- Qmedium (no air flooding). ....~

LAMBDA MINIFOR Fermentor-Bioreactor

How is it possible?By eliminating the critical points causingcontamination in common fermentors andbioreactors.

LAMBDA MINIFOR offers:/ Complete physical closure of the

v vessel and the side necks/ LAMBDA double-seal PEEK tubing

v connector/ Permanent stoppers with multiple

v seals-/ High quality Peristaltic Pumps for

long term continuous runs_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _4

Unique construction for easy sterilityr;;=.~~~

LAMBDA's uniquesterility constructionassures you withperfect and easysterility maintenancefor each and everyrun ... V

Vat LOWEST COST

~ LABORATORY INSTRUMENTS

Efficient IR heaterintegrated inside the

control unit

Easily accessibleconnections:

Probes and ports areat an angle of 30° for

easy ergonomichandling

Measurement and regulation of °C,pH, p02, air flow rate, stirring and aselectable parameter 'X'

Minimum bioreactor foot print ­comparable to the size of an A4sheet of paper (220 x 400 mm)

Weight of only 7.5 kg - watch itclosely, it could be carried awayeven by a child!

Covers the whole laboratory-scaleculture volume range from 35 ml toover 6 liters

Ex.rellD..eJlyCOlID..]pBC.Sys.ellD..

Parameterregulation andmonitoring at asingle glance

Pumps are placedat the rear to keep

lines short

Storage bottles aresecurely placed inmagnetic holders

behind thefermenter vessel

Do you haue space /jor asheet oli A4 paper onyour la60ratory bench?

Then, there's stillenoughc'ace /jor theMINIFO /jermentorbioreactor!

Perfect parameter control and smallest footprint

EASY-TO-USE AND ECONOMICAL SOLUTION

Switch volumes at low costInterchange vessel volumes (35 ml to over 61)with just one instrument

Threaded glass vessel with screw caps~.. . Setting up is as easy as screwing a cap on the

·4 ; ,f U bottlefor Unique sterility concept- L-, Assures a perfect & easy sterility

maintenance for a long time

Novel non-rotational bio-mixingProduces no vortex which eliminatesthe need of baffles

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WHY LAMBDA ELIMINATED HEAD PLATES?~COMP~ICATED setting up~ &cleaning process

~ EXPENSIVE spare parts

<01TIME consuming set-up

~\I DIFFICULT to make tightconnection with shallowthreads

LIMITED number of ports 10)REPLACE O-rings IO~before each run

CONTAMINATION dueto flattening of O-ringsduring sterilization

NEED new head plates~(probes, O-rings, nuts..) ~for each new vessel size ~

E;1 LARGE VESSEL = high medium cost + downstream cost + long sterilization &cooling time +.. difficulty in disposing infective material

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UP AND DOWN AGITATION Why LAMBDA replaced the traditional rotational stirring with

a unique and efficient agitation?

Advantages of LAMBDA's non-rotational agitation /­I J , ..1~

Quality sealing allows to maintain sterility easily for

long continuous runs

/-,I' ..~

J Increased cell viability with

optimal bio-mimicking "fish-tail" ag itation

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Saves money, simplifies Effective & No complex the setting up process and economical head-plates reduces cleaning time

sealing & baffles

+ + •

Efficient mixing for all types of culture : bacterial , mammal ian & fungal cells

Cells are not des troyed due to shear stress

Optimal oxygen transfer (OT R) without air flood ing

Induces hydrodynamic ~ shear stress to Destructive effect on

cells culture growth

Unreliable seal s to ~ High risk of contamination close the moving moto r axis and

immobile vessel

Increased handling complexity, downtime, foaming and complex internal environment

Vortex controlling ---p-. baffles compl icate -----/

the set-up &Air clean ing process floo ding

Inefficient gas &mass transfer in the media

oUnprecedentedvolume rangefrom 35 ml toover 6Iitres...

LAMBDA MINIFOR Fermentor-BioreactorI

¢ SWITCH VESSEL VOLUMES at low cost

0.3 L.... 7L....

.. Economical & Flexible solution at laboratory stage

.. Elimination of complex and expensive head plates

.. Optimized, user friendly vessel design

.. Unique ergonomic easy sterility concept minimizing contamination

.. Reduced heating, cooling and sterilization times

.. Perfect visibility into the vessel

.. Easy and precise control of the process parameters at any volume

.. Modular and cost efficient construction

.. Better work productivity due to the elimination of contamination risk

• ~ LABORATORY INSTRUMENTS

Infra-red heat rays provide softheating and optimal temperature

maintenance

SUN -LIKE WAY OF HEATING

x A big "NO" to heating blankets, jacketedvessels and thermal circulating baths

Why?

X Very expensive and voluminous

X Overheating of the medium

X Prevents natural cooling and acomplementary cooling is required

X Restricted view into the vessel

More heat is dissipated outside theX vessel (to your lab) than transmitted into

the medium

Heat transmission occurs mainly byslow and inefficient thermal conduction

How infra-red radiation heating is efficient?Precise temperaturecontrol at low cost

Natural upwardsconvection even

without any agitation

Imitating soft sun-likeway of heating

No hot spots at anymedium volume

Perfect visibility intothe vessels

Heats up and coolsdown quickly

Convenient & Safe - Nocables, tubings, connectors,

circulating water supplies

~ lABORATORYIN,TRU_ENT'

Presence of foam is detectedby the measurement of increasein electrical conductivity.

Save costave space

A No need of an expensiveV anti-foam probe

A Simply two needles of theV fermentor vessel are used as

electrodes

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_ The antifoam detector/controller

_ Detects the presence of foam

_ Sends control signals toDOZITO

_ No additional port needed forthe probe

_ Miniature Syringe Pump

_ Adds antifoam agent basedon the received signals

_ Prevents overdosing ofantifoam liquid

_ New motion principle

Watch your cells grow!Pump flow INTEGRATOR - an economical alternative to

Optical Density (00) measurements

During fermentation, cells produce acid or basewhich needs to be compensated.

The INTEGRATOR in the pump integrates theamount of added acid or base.

These data allow to assess cell growth orbiotransformation with a precision similar to titration.

Advantages you get:

~ Unlike in 00 measurements, the results arenot affected with the presence of dead cells,cell debris, air bubbles, precipitation & coloration

~ Precise, easy-to-use & maintenance free solutionat low cost

~ Integrated within the pumps to save yourvaluable bench-space

Growth rate and Biomass as a function of time

Where to use the INTEGRATOR?Titrate your cell's

METABOLIC ACTIVITY!

• Control and quantification of the metabolic activity of cells duringfermentation and cell culturesEg. by control of pH, rH, p02, pC02, conductivity or other parameters

• Measurement of the enzyme activity of numerous enzymesEg. esterases, acylases, lipases, proteases and others using a pH stat

• Recording foam formation (automatic antifoam addition)

• Recording the addition of reactants during exothermic reactionsby a thermostat

• Recording of the consumption of reactants during titrationsand much more applications

>70'I

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~ LABORATORY INSTRUMENTS EJ D

• Free selection of gases

• Individual flow rates per gas

• Pressure independent flow rate measurement

• Stand-alone & programmable or remote controlby industrial fermentation software SIAM

Which gas do your cells need?

p02 control & O2enrichment

pH control by CO 2

Constant gas flow

MINI -GAS isas easy to

put together aspuzzle pieces

High quality andcost effectivesetup

Each MASSFLOWwill control onegas in yourgas-mixture

MINI-4-GASis modular

According toyour project, youcan choose:

- upto 4 gases ofyour choice

- in 2 differentranges upto 500or 5000 ml/min

Why MASSFLOW?pH [oj p0 2 [mg/I) Total flow (I/min)

C02 Flow [I/min)

1--Air Flow (I/min)

I"'---"~'I0 2 Flow [I/min)

1--N2 Flow [I/min)

1--1iiiii=~,1

Specially developed for the precise flowmeasurement and control of gases

Uses a high quality laminar mass flow sensorwith a very low pressure drop

Flow rate is regulated by a special proprietaryproportional needle valve controlled by amicroprocessor

Flow rate is programmable (up to 50 pairs offlow rates and times)

Transferred gas volume can be totalized withthe INTEGRATOR

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MINIFOR provides a high quality, inexpensive & automaticweight-control solution for continuous cultures

I STAT

Miniature scale module (steel tip) placedunder MINIFOR for easy weight control

Pump head with large ball bearing rollersand asymmetric design decreasespulsation and increases tubing life

Precise & high quality weight control with GLPand GMP conform

Weight control independent of agitation, aeration,temperature, foaming, gas uptake & pressure

Efficient optimization of experiments in hoursinstead of days / weeks

Considerably higher productivity, e.g., yield of 3Lcontinuous culture is equal to 30L batch culture

High quality & reliable peristaltic pumps specially ,-----------,developed for long term continuous processes

Constant flow rate for several weeks with no riskof tubing rupture or other malfunctions

6. Choose efficient sparger with non-blockable fine pores

Submerged aeration in cell culture needs an efficient sparger which is not blocked by deposits.

Self-cleaning microsparger of MINIFOR for long runs (for ego months) with efficient sparging

8. Never choose a mono­speed pumps with stoppers

for long runs LAMBDA pumps are specially developed for long term continuous cell cultures with reduced pulsation and stable flow rates

2. Select smallest volume possible

Growth & production media is extremely expensive. MINIFOR bioreactor allows to work with smallest working volume of 35ml to test the culture models

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Bioreactor for aaimal cell culture While purchasing a bioreactor (stem cell):

For CHO, HeLa, HEK-293 and other cell lines consider gentle mixing , smallest volume possible , sterility, ideal gas mix, efficient

gas exchange, exchangeable vessel volume at low cost, peristaltic pumps for long runs, precise temperature control

4. Human factor and Ergonomics (HF & E)

Bioreactor should be '-_..... safe to be handled by

all the technical people working with it. Work with MINIFOR without depending on GLPIGMP trained technician