Upload
roland
View
229
Download
5
Tags:
Embed Size (px)
DESCRIPTION
Flagella
Citation preview
Lab 2
Flagella Regeneration
in Chlamydomonas reinhardtii
or how simple microscopy can be used to calculate rates of assembly of macromolecules into cellular structures
Andrew, Jiayun, Michael, Madeline
Fri Allens sec.
Bharat, John, Russell & Fahd
Fri Kais sec
Rubi, Michelle, David & Raj
Fri Kais sec
Chlamy fixed in Lugols Iodine
1000X phase contrast
experiment
Remove flagella with a pH shock, pH 7.5 to 4.5 for 60s then back to pH 7.5In teams of 4, design an experiment to determine the effect of a drug on flagella regeneration.Two flasks, one control, one with a drug.Possible drugs are calcium, lithium, cycloheximide, actinomycin D or caffeine.Illuminate for one hour (or more if needed), taking and fixing samples every 15 min.Mount samples and measure flagella lengths.Determine rate of flagella growth in micrometers per minute.the flagella
Electron MicrographDiagramatic Drawing
The axoneme is composed of a 9 + 2
structure of microtubules.
Each of the nine outer are partial doubles,
one complete microtubule with 13
protofilaments and one partial microtubule
with 10 protofilaments.
The two inner are complete microtubules.
From Iowa State University Agronomy 317
Actinophrys,
a heliozoan protist.
The heliozoan has a spherical cell body with thick radiating
strands of cytoplasm called axopodia, which are supported by
microtubules. When smaller organisms bump into the axopodia, they
stick to the axopodia and are then moved down the axopodia to the
cell body, where they are phagocytosed.
http://www.microscopy-uk.org.uk/mag/imgfeb02/acti3web.jpg
Ref.: http://www1.umn.edu/news/prod/groups/ur/@pub
/@ur/documents/asset/ur_87651.jpg
Actinophrys
Chlamydomonas
axopodia
flagella
finis