Embed Size (px)
Citation preview
![Page 1: Flow Cytometry Preliminary Report Shepard...SS INT LIN 10 0 10 1 10 2 10 3 10 4 0 256 512 768 1024 SYTO[+], 92.4% COUNT - 45KrO Live CD45-KrO SS INT LIN 10 0 10 1 10 2 10 3 10 4 0](https://reader035.pdfslide.net/reader035/viewer/2022070511/611194227d8ddf276656a144/html5/thumbnails/1.jpg)
Page 1 of 16CDFW21-133 26-Feb-21 FRANCKE ROBERT KT
[ ] Histogram image saved[ ] CoPath Medfusion[ ] CoPath San Antonio[ ] CoPath Austin[ ] CoPath Sammons[ ] CoPath Fairfax[ ] CoPath Norfolk[ ] CoPath RMCC[ ] CoPath MOH[ ] CoPath Irving Labs - JPG
[ ] CoPath Good Sheppard - JPG
[ ] CoPath Midland Pathlgy - JPG
[ ] CoPath Ameripath - JPG
[ ] CoPath CDH - JPG
Case: CDFW21-133
Patient Name: FRANCKE ROBERT KT
ICD: thrombocytopenia
Analyst: XD Technologist: KT
Flow CytometryPreliminary Report
Results
Specimen Type: Peripheral Blood
Other cells:
0.1% Myeloblasts3.1% Polyclonal B-cells, Kappa/Lambda ratio=1.4:1 18.9% Small T-cells, CD4:CD8 ratio=3.4:1 6.3% Natural Killer (NK) cells 4.2% Mature Monocytes0.1% Immature monocytes62.0% Neutrophils1.3% Eosinophils <0.1% Plasma cellsErythroid cells, CD45 [-] cells & debris comprise the remaining events
Viability: 96% Cellularity: 2.9 x10^6 cells / mL, 3.0 mL total specimen
Markers analyzed: CD2, CD3, CD4, CD5, CD7, CD8, CD10, CD11b, CD13, CD14, CD16, CD19, CD20, CD33,CD34, CD38, CD45, CD56, CD64, CD117, CD123, HLA-DR, Kappa, Lambda
____________________________________________________________________________
Interpretation
Flow Cytometric Immunophenotype: No immunophenotypic abnormalities detected
![Page 2: Flow Cytometry Preliminary Report Shepard...SS INT LIN 10 0 10 1 10 2 10 3 10 4 0 256 512 768 1024 SYTO[+], 92.4% COUNT - 45KrO Live CD45-KrO SS INT LIN 10 0 10 1 10 2 10 3 10 4 0](https://reader035.pdfslide.net/reader035/viewer/2022070511/611194227d8ddf276656a144/html5/thumbnails/2.jpg)
Page 2 of 16CDFW21-133 26-Feb-21 FRANCKE ROBERT KT
T & NK Cells
Description AlertCD3 T cells 18.9% OK
CD5 T cells 18.4% OK
Large CD3 T cells 0.2% OK
CD4 + CD8 T cells 17.8% OK
CD4/CD8 Co-exp %T 0.1% OK
CD4 : CD8 Ratio 3.4 OK
CD3[-]CD7+ NK cells 6.3% OK
CD11b+CD56+ NK 9.3% OK
CD3+CD7[-] T cells 1.1% OK
CD3+CD2[-] T cells 0.1% OK
B Cells
Description Alert
Kappa:Lambda Ratio 1.4 OK
sIg[-] B Cells 0.2% OK
Large B Cells 0.1% OK
K/L ratio (largeBcells) 0.3 OK
CD5/CD19 co-exp 10.0% ALERT >5%
CD10/CD19 co-exp 0.4% OK
CD5/CD10 co-exp 0.1% OK
Plasma Cells 0.0% OK
Polyclonal B if there is aLambda clone
3.2% N A
Polyclonal B if there is aKappa clone
3.5% N A
Myeloid Cells / Myelodysplasia
Description Alert
CD34 Myeloblasts 0.1% OK
CD117 Myeloblasts 0.0% OK
CD13+CD16[-] Eosinophils 1.3% OK
CD10[-]SS+ Eosinophils 1.3% OK
CD10[-] Left-Shift 1.3% OK
CD123+ Low SS Cells 0.9% OK
CD11b[pos] + 11b[neg] Neutrophils 62.0% OK
CD16[-]CD13+ Neutrophils Left-Shift ([-] Eos) 0.9% OK
CD45+SS+ Neutrophils 62.0% OK
CD56+SS+ Neutrophils 4.2% ALERT >1%
CD117+ Neutrophils 0.2% OK
CD11b[-]SS+ Promyelocytes 0.1% OK
CD14[-]CD64+ Immature Monocytes 0.1% OK
CD56+ Monocytes 0.4% OK
CD16+ Monocytes 0.8% OK
Cytosum: 96.0% Viability: 95.5% OK CALFACTOR: 997
Flow Cytometry Statistics and Alerts
Average Live = 79.2%, FAIL<85% NOT RBC1 = 97.2%, OK
![Page 3: Flow Cytometry Preliminary Report Shepard...SS INT LIN 10 0 10 1 10 2 10 3 10 4 0 256 512 768 1024 SYTO[+], 92.4% COUNT - 45KrO Live CD45-KrO SS INT LIN 10 0 10 1 10 2 10 3 10 4 0](https://reader035.pdfslide.net/reader035/viewer/2022070511/611194227d8ddf276656a144/html5/thumbnails/3.jpg)
Page 3 of 16CDFW21-133 26-Feb-21 FRANCKE ROBERT KT
Count & Viability TubeCount Instrument S/N: BD07525
COUNT - 45KrO No Gate
Syto13
SS
IN
T L
IN
100
101
102
103
104
0
256
512
768
1024SYTO[+], 92.4%
COUNT - 45KrO Live
CD45-KrO
SS
IN
T L
IN
100
101
102
103
104
0
256
512
768
1024
COUNT - 45KrO Live
FS INT LIN
SS
IN
T L
IN
0 256 512 768 10240
256
512
768
1024Apoptotic, 2%
COUNT - 45KrO SYTO[+]
7AAD
FS
IN
T L
IN
100
101
102
103
104
0
256
512
768
1024Live = 96%
QA notesCluster positions> OK
![Page 4: Flow Cytometry Preliminary Report Shepard...SS INT LIN 10 0 10 1 10 2 10 3 10 4 0 256 512 768 1024 SYTO[+], 92.4% COUNT - 45KrO Live CD45-KrO SS INT LIN 10 0 10 1 10 2 10 3 10 4 0](https://reader035.pdfslide.net/reader035/viewer/2022070511/611194227d8ddf276656a144/html5/thumbnails/4.jpg)
Page 4 of 16CDFW21-133 26-Feb-21 FRANCKE ROBERT KT
B Cell Tube Pg 1Lambda FITC - Kappa PE - X - ECD - CD38 PC5.5 -CD5 PC7 - CD10 APC - CD34 AA700 - CD19 AA750 -CD20 Pac Blue -
CD45 KrO
Note: Instrument S/N Tube #1: BB27034 Cluster Positions: OK
B Cell Singlets 1
FS INT LIN
SS
IN
T L
IN
0 256 512 768 10240
256
512
768
1024
Lymphs124.9%
Live181%
B Cell RBC1
CD45 KrO
SS
IN
T L
IN
100
101
102
103
104
0
256
512
768
1024
Cells1100%
COI 10.0%
B Cell Cells1
CD45 KrO
CD
19 A
A750
100
101
102
103
104
100
101
102
103
104
19+ B Cells3.6%
B Cell Cells1
CD20 Pac Blue
SS
IN
T L
IN
100
101
102
103
104
0
256
512
768
1024CD20 B cells,1942 events,3.1%
B Cell CD20 B cells
Kappa PE
CD
20 P
ac
Blu
e
100
101
102
103
104
100
101
102
103
104
K:L Ratio = 1.4
0%0%
63%
1217 events
37%
725 events
B Cell CD20 B cells
Lambda FITC
CD
20 P
ac
Blu
e
100
101
102
103
104
100
101
102
103
104
0%0%
45%
872 events55%
1070 events
![Page 5: Flow Cytometry Preliminary Report Shepard...SS INT LIN 10 0 10 1 10 2 10 3 10 4 0 256 512 768 1024 SYTO[+], 92.4% COUNT - 45KrO Live CD45-KrO SS INT LIN 10 0 10 1 10 2 10 3 10 4 0](https://reader035.pdfslide.net/reader035/viewer/2022070511/611194227d8ddf276656a144/html5/thumbnails/5.jpg)
Page 5 of 16CDFW21-133 26-Feb-21 FRANCKE ROBERT KT
B Cell Tube Pg 2Lambda FITC - Kappa PE - X - ECD - CD38 PC5.5 -CD5 PC7 - CD10 APC - CD34 AA700 - CD19 AA750 -CD20 Pac Blue -
CD45 KrO
Note: Instrument S/N Tube #1: BB27034 Cluster Positions: OK
B Cell Cells1
CD45 KrO
CD
19
AA
75
0
100
101
102
103
104
100
101
102
103
104
19+ B Cells3.6%
B Cell 19+ B Cells
FS INT LIN
CD
20 P
ac B
lue
0 256 512 768 102410
0
101
102
103
104
Large B Cells, 3%, 71 cells
SmallBcells, 90%, 2003 cells
B Cell SmallBcells
Lambda FITC
Kappa P
E
100
101
102
103
104
100
101
102
103
104
38%2%
2%57%
B Cell Large B Cells
Lambda FITC
Kappa P
E
100
101
102
103
104
100
101
102
103
104
Large Cell
K/L ratio = 0.3
30%0%
61%10%Small Cell
K/L ratio = 1.5
B Cell CD38+ PC's
CD20 Pac Blue
CD
19 A
A750
100
101
102
103
104
100
101
102
103
104
0% 0%
0% 0%
B Cell Live1
CD45 KrO
CD
38
PC
5.5
100
101
102
103
104
100
101
102
103
104
RBC197.2%
CD38+ PC's0.0%
![Page 6: Flow Cytometry Preliminary Report Shepard...SS INT LIN 10 0 10 1 10 2 10 3 10 4 0 256 512 768 1024 SYTO[+], 92.4% COUNT - 45KrO Live CD45-KrO SS INT LIN 10 0 10 1 10 2 10 3 10 4 0](https://reader035.pdfslide.net/reader035/viewer/2022070511/611194227d8ddf276656a144/html5/thumbnails/6.jpg)
Page 6 of 16CDFW21-133 26-Feb-21 FRANCKE ROBERT KT
B Cell Tube Pg 3Lambda FITC - Kappa PE - X - ECD - CD38 PC5.5 -CD5 PC7 - CD10 APC - CD34 AA700 - CD19 AA750 -CD20 Pac Blue -
CD45 KrO
Note: Instrument S/N Tube #1: BB27034 Cluster Positions: OK
B Cell Lymphs1
CD5 PC7
CD
20 P
ac B
lue
100
101
102
103
104
100
101
102
103
104
B+5+,
10.0% of Mature B,
0.3% T
B Cell B+5+
Lambda FITC
Kappa P
E
100
101
102
103
104
100
101
102
103
104
36.60%1.55%
1.55%60.31% B+5+ K/L Ratio =1.6
B Cell Lymphs1
CD10 APC
CD
20 P
ac
Blu
e
100
101
102
103
104
100
101
102
103
104
B+10+,
0.4% of Mature B,
0.0% T
B Cell B+10+
Lambda FITC
Kappa P
E
100
101
102
103
104
100
101
102
103
104
14% 14%
0% 71%
B+10+ K/L Ratio = 0.2
B Cell CD20 B cells
CD5 PC7
CD
20 P
ac
Blu
e
100
101
102
103
104
100
101
102
103
104
90% 9.84%
of Mature B
0.00% 0.00%
B Cell CD20 B cells
CD10 APC
CD
20 P
ac
Blu
e
100
101
102
103
104
100
101
102
103
104
99.59% 0.41%
of Mature B
0.00% 0.00%
![Page 7: Flow Cytometry Preliminary Report Shepard...SS INT LIN 10 0 10 1 10 2 10 3 10 4 0 256 512 768 1024 SYTO[+], 92.4% COUNT - 45KrO Live CD45-KrO SS INT LIN 10 0 10 1 10 2 10 3 10 4 0](https://reader035.pdfslide.net/reader035/viewer/2022070511/611194227d8ddf276656a144/html5/thumbnails/7.jpg)
Page 7 of 16CDFW21-133 26-Feb-21 FRANCKE ROBERT KT
B Cell Tube Pg 4Lambda FITC - Kappa PE - X - ECD - CD38 PC5.5 -CD5 PC7 - CD10 APC - CD34 AA700 - CD19 AA750 -CD20 Pac Blue -
CD45 KrO
Note: Instrument S/N Tube #1: BB27034 Cluster Positions: OK
B Cell Cells1
CD19 AA750
SS
IN
T L
IN
100
101
102
103
104
0
256
512
768
1024
CD19 Low SS3.28%
B Cell Cells1
CD10 APC
SS
IN
T L
IN
100
101
102
103
104
0
256
512
768
1024
Low SS 10+Pre-Bor10+ Lymphoma0.0%
B Cell CD19 Low SS
Lambda FITC
Kappa P
E
100
101
102
103
104
100
101
102
103
104
56% 1%
3% 40%
B Cell Cells1
CD5 PC7
CD
19
AA
75
0
100
101
102
103
104
100
101
102
103
104
CD5+ T Cells18.4%
B Cell Cells1
CD10 APC
CD
5 P
C7
100
101
102
103
104
100
101
102
103
104
CD10+ CD5 Cells0.1% T
All 19+ Cells(CD19 Low SS)K:L Ratio = 1.4
![Page 8: Flow Cytometry Preliminary Report Shepard...SS INT LIN 10 0 10 1 10 2 10 3 10 4 0 256 512 768 1024 SYTO[+], 92.4% COUNT - 45KrO Live CD45-KrO SS INT LIN 10 0 10 1 10 2 10 3 10 4 0](https://reader035.pdfslide.net/reader035/viewer/2022070511/611194227d8ddf276656a144/html5/thumbnails/8.jpg)
Page 8 of 16CDFW21-133 26-Feb-21 FRANCKE ROBERT KT
B Cell Cells1
CD34 AA700
SS
IN
T L
IN
100
101
102
103
104
0
256
512
768
1024
CD34+0.0%
B Cell RBC1
CD45 KrO
SS
IN
T L
IN
100
101
102
103
104
0
256
512
768
1024Grans163.3%- 1.3% 10- Eos
= 62.0% neutrophils
Monos14.8%
45Lymphs129.4%
B Cell Cells1
CD45 KrO
CD
10 A
PC
100
101
102
103
104
100
101
102
103
104
50%0%
50%0%
Pre-B
B Cell Grans1
CD45 KrO
CD
10 A
PC
100
101
102
103
104
100
101
102
103
104
CD10- Grans [LS], 1.3% of Grans
CD10- Eos, 2.1% of Grans, 1.3% T
B Cell Cells1
CD10 APC
CD
34 A
A700
100
101
102
103
104
100
101
102
103
104
2% 2%
50% 46%
B Cell Cells1
CD19 AA750
CD
34 A
A700
100
101
102
103
104
100
101
102
103
104
4% 0%
94% 2%
B Cell Tube Pg 5Lambda FITC - Kappa PE - X - ECD - CD38 PC5.5 -CD5 PC7 - CD10 APC - CD34 AA700 - CD19 AA750 -CD20 Pac Blue -
CD45 KrO
Note: Instrument S/N Tube #1: BB27034 Cluster Positions: OK
![Page 9: Flow Cytometry Preliminary Report Shepard...SS INT LIN 10 0 10 1 10 2 10 3 10 4 0 256 512 768 1024 SYTO[+], 92.4% COUNT - 45KrO Live CD45-KrO SS INT LIN 10 0 10 1 10 2 10 3 10 4 0](https://reader035.pdfslide.net/reader035/viewer/2022070511/611194227d8ddf276656a144/html5/thumbnails/9.jpg)
Page 9 of 16CDFW21-133 26-Feb-21 FRANCKE ROBERT KT
T Cell Tube Pg 1CD11b FITC - CD7 PE - CD2 ECD - CD56 PC5.5 -CD5 PC7 - CD16 APC - CD4 AA700 - CD3 AA750 -CD8 Pac Blue -
CD45 KrO
Note: Instrument S/N Tube #2: BB27034 Cluster Positions: OK
T Cell Singlets 2
FS INT LIN
SS
IN
T L
IN
0 256 512 768 10240
256
512
768
1024Live280.0%
T Cell Live2
CD45 KrO
SS
IN
T L
IN
100
101
102
103
104
0
256
512
768
1024
Lymphs232.3%
Cells298%
COI 20.0%
T Cell Cells2
CD45 KrO
CD
2 E
CD
100
101
102
103
104
100
101
102
103
104
T Cell Cells2
CD45 KrO
CD
7 P
E
100
101
102
103
104
100
101
102
103
104
T Cell Cells2
FS INT LIN
CD
3 A
A7
50
0 256 512 768 102410
0
101
102
103
104
CD318.9%
Large CD30.2%
T Cell CD3
CD7 PE
CD
2 E
CD
100
101
102
103
104
100
101
102
103
104
3+7-,6%,1.1T
93%
0% 1%
![Page 10: Flow Cytometry Preliminary Report Shepard...SS INT LIN 10 0 10 1 10 2 10 3 10 4 0 256 512 768 1024 SYTO[+], 92.4% COUNT - 45KrO Live CD45-KrO SS INT LIN 10 0 10 1 10 2 10 3 10 4 0](https://reader035.pdfslide.net/reader035/viewer/2022070511/611194227d8ddf276656a144/html5/thumbnails/10.jpg)
Page 10 of 16CDFW21-133 26-Feb-21 FRANCKE ROBERT KT
T Cell Tube Pg 2CD11b FITC - CD7 PE - CD2 ECD - CD56 PC5.5 -CD5 PC7 - CD16 APC - CD4 AA700 - CD3 AA750 -CD8 Pac Blue -
CD45 KrO
Note: Instrument S/N Tube #2: BB27034 Cluster Positions: OK
T Cell Cells2
FS INT LIN
CD
2 E
CD
0 256 512 768 102410
0
101
102
103
104
CD2+25.74%
T Cell CD2+
CD7 PE
CD
3 A
A7
50
100
101
102
103
104
100
101
102
103
104
NK Cells,24%,6.3% T2%
68%5%
T Cell Cells2
FS INT LIN
CD
4 A
A700
0 256 512 768 102410
0
101
102
103
104
CD4+13.8%
T Cell Cells2
FS INT LIN
CD
8 P
ac
Blu
e
0 256 512 768 102410
0
101
102
103
104
CD8+4.0%
T Cell Lymphs2
CD8 Pac Blue
CD
4 A
A7
00
100
101
102
103
104
100
101
102
103
104
CD4:CD8 Ratio = 3.4
20%33%
CD4/CD8 Dual+,0%, 0.1%T
47%
![Page 11: Flow Cytometry Preliminary Report Shepard...SS INT LIN 10 0 10 1 10 2 10 3 10 4 0 256 512 768 1024 SYTO[+], 92.4% COUNT - 45KrO Live CD45-KrO SS INT LIN 10 0 10 1 10 2 10 3 10 4 0](https://reader035.pdfslide.net/reader035/viewer/2022070511/611194227d8ddf276656a144/html5/thumbnails/11.jpg)
Page 11 of 16CDFW21-133 26-Feb-21 FRANCKE ROBERT KT
T Cell Tube Pg 3CD11b FITC - CD7 PE - CD2 ECD - CD56 PC5.5 -CD5 PC7 - CD16 APC - CD4 AA700 - CD3 AA750 -CD8 Pac Blue -
CD45 KrO
Note: Instrument S/N Tube #2: BB27034 Cluster Positions: OK
T Cell Cells2
CD5 PC7
SS
IN
T L
IN
100
101
102
103
104
0
256
512
768
1024
CD5 (T-cell tube)19.25%
T Cell CD3
CD5 PC7
CD
3 A
A7
50
100
101
102
103
104
100
101
102
103
104
0.00%0.00%
97.65%2.35%
CD3+CD5-0.4% T
T Cell CD3+CD5-
CD8 Pac Blue
CD
4 A
A700
100
101
102
103
104
100
101
102
103
104
21%
40% 27%
CD3+CD5- T Cells
CD4:CD8 Ratio = 0.4
11%
T Cell CD3+CD7-
CD8 Pac Blue
CD
4 A
A700
100
101
102
103
104
100
101
102
103
104
77%
CD3+CD7- T Cells
CD4:CD8 Ratio = 6.8
11%12%
0%
T Cell CD3
CD7 PE
CD
2 E
CD
100
101
102
103
104
100
101
102
103
104
CD3+CD7-7%,
1.1T
![Page 12: Flow Cytometry Preliminary Report Shepard...SS INT LIN 10 0 10 1 10 2 10 3 10 4 0 256 512 768 1024 SYTO[+], 92.4% COUNT - 45KrO Live CD45-KrO SS INT LIN 10 0 10 1 10 2 10 3 10 4 0](https://reader035.pdfslide.net/reader035/viewer/2022070511/611194227d8ddf276656a144/html5/thumbnails/12.jpg)
Page 12 of 16CDFW21-133 26-Feb-21 FRANCKE ROBERT KT
T Cell Tube Pg 4 - Myeloid MarkersCD11b FITC - CD7 PE - CD2 ECD - CD56 PC5.5 -CD5 PC7 - CD16 APC - CD4 AA700 - CD3 AA750 -CD8 Pac Blue -
CD45 KrO
Note: Instrument S/N Tube #2: BB27034 Cluster Positions: OK
T Cell Cells2
CD56 PC5.5
CD
11
b F
ITC
100
101
102
103
104
100
101
102
103
104
CD11b/CD56 NK9.3%
T Cell Cells2
CD56 PC5.5
SS
IN
T L
IN
100
101
102
103
104
0
256
512
768
1024
CD56+ Monos0.4%
56+ Grans4.2%
T Cell Cells2
CD16 APC
SS
IN
T L
IN
100
101
102
103
104
0
256
512
768
1024
CD16 SS8.1%
T Cell Live2
CD45 KrO
CD
56 P
C5.5
100
101
102
103
104
100
101
102
103
104
CD56+ PC's0.0%
T Cell Cells2
FS INT LIN
CD
11
b F
ITC
0 256 512 768 102410
0
101
102
103
104
Promyelo, 0.1%
CD11BGrans, 61.9%
T Cell Cells2
CD45 KrO
CD
11
b F
ITC
100
101
102
103
104
100
101
102
103
104
Total
Grans,
62.0%
![Page 13: Flow Cytometry Preliminary Report Shepard...SS INT LIN 10 0 10 1 10 2 10 3 10 4 0 256 512 768 1024 SYTO[+], 92.4% COUNT - 45KrO Live CD45-KrO SS INT LIN 10 0 10 1 10 2 10 3 10 4 0](https://reader035.pdfslide.net/reader035/viewer/2022070511/611194227d8ddf276656a144/html5/thumbnails/13.jpg)
Page 13 of 16CDFW21-133 26-Feb-21 FRANCKE ROBERT KT
Myeloid Tube Pg 1CD16 FITC - CD123 PE - CD64 ECD - CD13 PC5.5 - CD117 PC7- CD33 APC - CD34 AA700 - CD14 AA750 -
HLA-DR Pac Blue - CD45 KrO
Note: Instrument S/N Tube #3: BB27034 Cluster Positions: OK
Myeloid tube Singlets 3
FS INT LIN
SS
IN
T L
IN
0 256 512 768 10240
256
512
768
1024
Live376.4%
Myeloid tube RBC3
CD45 KrO
SS
IN
T L
IN
100
101
102
103
104
0
256
512
768
1024
Cells3100%
COI 30.0%
Myeloid tube Cells3
CD34 AA700
SS
IN
T L
IN
100
101
102
103
104
0
256
512
768
1024
CD34 BL0.1%
Myeloid tube CD34 BL
CD117 PC7
CD
34 A
A700
100
101
102
103
104
100
101
102
103
104
0%0%
53%47%
Myeloid tube Cells3
CD117 PC7
SS
IN
T L
IN
100
101
102
103
104
0
256
512
768
1024
Large 1170.2%
CD117 BL0.0%
Myeloid tube Cells3
CD16 FITC
CD
34
AA
70
0
100
101
102
103
104
100
101
102
103
104
0.1%
70%28%
2%
![Page 14: Flow Cytometry Preliminary Report Shepard...SS INT LIN 10 0 10 1 10 2 10 3 10 4 0 256 512 768 1024 SYTO[+], 92.4% COUNT - 45KrO Live CD45-KrO SS INT LIN 10 0 10 1 10 2 10 3 10 4 0](https://reader035.pdfslide.net/reader035/viewer/2022070511/611194227d8ddf276656a144/html5/thumbnails/14.jpg)
Page 14 of 16CDFW21-133 26-Feb-21 FRANCKE ROBERT KT
Myeloid Tube Pg 2CD16 FITC - CD123 PE - CD64 ECD - CD13 PC5.5 - CD117 PC7- CD33 APC - CD34 AA700 - CD14 AA750 -
HLA-DR Pac Blue - CD45 KrO
Note: Instrument S/N Tube #3: BB27034 Cluster Positions: OK
Myeloid tube Cells3
CD123 PE
SS
IN
T L
IN
100
101
102
103
104
0
256
512
768
1024
Baso&Dendritic Cells0.9%
Myeloid tube Cells3
CD45 KrO
SS
IN
T L
IN
100
101
102
103
104
0
256
512
768
1024
Grans360.8%
Myeloid tube PNH? Cells3
CD16 FITC
SS
IN
T L
IN
100
101
102
103
104
0
256
512
768
1024
16+ Monos0.8%
16+ Grans59.1%
Myeloid tube Grans3
CD16 FITC
CD
13 P
C5.5
100
101
102
103
104
100
101
102
103
104
CD16[-]Grans3.0%
Myeloid tube CD16[-]Grans
CD13 PC5.5
SS
IN
T L
IN
100
101
102
103
104
0
256
512
768
1024
CD13Mo12.0% G,
3.7% T
CD13Eos4.2% G,1.3% T
Myeloid tube Cells3
CD45 KrO
HL
A-D
R P
ac
Blu
e
100
101
102
103
104
100
101
102
103
104
![Page 15: Flow Cytometry Preliminary Report Shepard...SS INT LIN 10 0 10 1 10 2 10 3 10 4 0 256 512 768 1024 SYTO[+], 92.4% COUNT - 45KrO Live CD45-KrO SS INT LIN 10 0 10 1 10 2 10 3 10 4 0](https://reader035.pdfslide.net/reader035/viewer/2022070511/611194227d8ddf276656a144/html5/thumbnails/15.jpg)
Page 15 of 16CDFW21-133 26-Feb-21 FRANCKE ROBERT KT
Myeloid Tube Pg 3CD16 FITC - CD123 PE - CD64 ECD - CD13 PC5.5 - CD117 PC7- CD33 APC - CD34 AA700 - CD14 AA750 -
HLA-DR Pac Blue - CD45 KrO
Note: Instrument S/N Tube #3: BB27034 Cluster Positions: OK
Myeloid tube Cells3
CD13 PC5.5
HL
A-D
R P
ac
Blu
e
100
101
102
103
104
100
101
102
103
104
DR Lymphs5.2%
Myeloid tube Cells3
CD45 KrO
SS
IN
T L
IN
100
101
102
103
104
0
256
512
768
1024
Myeloid tube Cells3
CD45 KrO
CD
33 A
PC
100
101
102
103
104
100
101
102
103
104
CD33+ Cells0.0%
Myeloid tube Cells3
CD14 AA750
SS
IN
T L
IN
100
101
102
103
104
0
256
512
768
1024
CD14Mo4.2%
Myeloid tube Cells3
CD33 APC
SS
IN
T L
IN
100
101
102
103
104
0
256
512
768
1024
CD33+ Mo/BL4.8%
Myeloid tube CD33+ Mo/BL
CD14 AA750
CD
33
AP
C
100
101
102
103
104
100
101
102
103
104
14-/33+ Cells12.7%,0.6%T
![Page 16: Flow Cytometry Preliminary Report Shepard...SS INT LIN 10 0 10 1 10 2 10 3 10 4 0 256 512 768 1024 SYTO[+], 92.4% COUNT - 45KrO Live CD45-KrO SS INT LIN 10 0 10 1 10 2 10 3 10 4 0](https://reader035.pdfslide.net/reader035/viewer/2022070511/611194227d8ddf276656a144/html5/thumbnails/16.jpg)
Page 16 of 16CDFW21-133 26-Feb-21 FRANCKE ROBERT KT
Myeloid Tube Pg 4CD16 FITC - CD123 PE - CD64 ECD - CD13 PC5.5 - CD117 PC7- CD33 APC - CD34 AA700 - CD14 AA750 -
HLA-DR Pac Blue - CD45 KrO
Note: Instrument S/N Tube #3: BB27034 Cluster Positions: OK
Myeloid tube Cells3
CD64 ECD
SS
IN
T L
IN
100
101
102
103
104
0
256
512
768
1024
CD64Mo3.8%
Myeloid tube CD64Mo
CD14 AA750
CD
33
AP
C
100
101
102
103
104
100
101
102
103
104
Immature Monos1.7%,0.1% T
Myeloid tube Cells3
CD45 KrO
CD
123 P
E
100
101
102
103
104
100
101
102
103
104
98.15%0.01%
1.84%0.00%
Myeloid tube Cells3
CD45 KrO
CD
34 A
A700
100
101
102
103
104
100
101
102
103
104
0% 2%
0% 98%
Myeloid tube Cells3
CD16 FITC
CD
11
7 P
C7
100
101
102
103
104
100
101
102
103
104
CD117+ Grans0.2%
Mast Cells0.0%
CD45 KrO
SS
IN
T L
IN
100
101
102
103
104
0
256
512
768
1024
Myeloid Blasts Picture
