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Flow Injection Analysis (FIA)
Flow Injection AnalysisFlow Injection Analysis
Chemiluminescence – Trace Metals ( e.g. dFe)
Spectrophotometric – Nutrients
S
T
HA
W
Time [S]
Flow Injection AnalysisFlow Injection Analysis
Typical FI detection response output: S is sampleinjection; W is peak width; A is peak areas; H ispeak height and T is residence time.
2
Co
Sample injection
Flow Injection AnalysisFlow Injection Analysis
Co
Sample injection
C max
Flow Injection AnalysisFlow Injection Analysis
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Flow Injection AnalysisFlow Injection Analysis
Sample
Waste
Waste
Acid
Buffer
ICP-MS
column
Flow Injection AnalysisFlow Injection Analysis
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Flow Injection AnalysisFlow Injection Analysis
Flow Injection AnalysisFlow Injection Analysis
0 50 100 150 200 250 300
-0.002
0.000
0.002
0.004
0.006
0.008
0.010
0.012
0.014
0.016
0.018
0.020
Absorb
ance (
A.U
.)
Time (sec)
Reproducibility
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Samplepump
NO
Sample loop
COMM COMM
Waste
Carrierpump
Ultra-purewater
NC NC NO
NC
COMM
NC
NO NO
Reagentpump
Ammoniummolybdate
Tin(II)chloride
Reagentpump
Coil 1 (30 cm)
Sample
T-piece
T-piece
Coil 2 (30 cm)
Coil 3 (90 cm)
Detector
COMM
Flow Injection AnalysisFlow Injection Analysis
- r2 = 0.998
- LOD = 0.67 µM PO4-P
- Linear Range = 0.8 - 50 µM - Sample throughput = 40 samples h-1
-200 0 200 400 600 800 1000 1200 1400 1600 -0.015
-0.010
-0.005
0.000
0.005
0.010
0.015
0.020
0.025
0.030
0.035
0.040
8.0 uM
6.0 uM
4.0 uM
1.5 uM
0.8 uM
-500 0 500 1000 1500 2000 2500 3000 3500 4000-0.10
-0.08
-0.06
-0.04
-0.02
0.00
0.02
0.04
0.06
0.08
0.10
Abs
orba
nce
(A.U
.)
Time (s)
Flow Injection AnalysisFlow Injection Analysis
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Flow Injection-Capillary Electrophoresis (FI-CE)
-CE: Excellent tool for examining many types of species includingproteins, DNA, sugars, cations and anions and drugs.
“Achilles Heel” – sample introduction
W
Pump
B
S
A
W
C
B
SV1 SV2
+-SC
SFC
-FI: Powerful sample introduction/manipulation tool
FI-CEPotential in clinical and forensic, biological, environmental andpharmaceutical analysis.
Flow Injection Analysis Flow Injection Analysis –– Capillary Electrophoresis Capillary Electrophoresis
Flow Injection AnalysisFlow Injection Analysis
www.ntmuk.edu/ce
Figure provided in part by: Dr. Gomez
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Detector
Separation capillary
200 μm Fiber Optic Cable
200 μm Fiber Optic Cable
Flow Cell
Electrolytereservoir
Plexiglass block
Flow Cell/Detector Set-up
Separation capillary
VoltagePt
Waste
FI Manifold
Hanrahan & Gomez, 2005