FluoroSENS - Fluorimeter

8/12/2019 FluoroSENS - Fluorimeter

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uoroSENS Brochure Issue 1

THE NEW STANDARD IN

HIGH SENSITIVITY FLUORESCENCE SPECTROSCOPY

fluoro SENS Sin gl e Pho to n

Sens i t i v i t y

Seeing is believing ...


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Single photon sensitivity guarantees great performance - industry leading water Raman signal-to- noise ratio for a bench-top uorimeter

The uoro SENS is a versatile bench-topuorimeter with single-photon counting

sensitivity. It smashes the established price-performance ratio for a high-sensitivityuorimeter.

The uoro SENS brings the ultimate sensitivityof single-photon counting into a fullyintegrated, computer-controlled spectrometer,allowing measurements of high-performancesteady-state uorescence spectra.

Ultimate photon counting sensitivity nowno longer for exclusive specialist research

Exceptionally high dynamic range weak

and strong uorescence peaks can beviewed simultaneously

High spectral resolution ne linestructure can be resolved with minimumstray light

Comprehensive software for spectralmeasurements, analysis and presentation

Many advanced performance featuresincluded as standard not usually found atthis price level

Full spectral correction for both emissionand excitation channels

Compact footprint ultimate scienticperformance packed into a bench-topinstrument

Complete luminescence laboratory in theone package - uorescence,phosphorescence, transmission, quantum

yields, electro-luminescence, plate reader,mapping systems, cryostats and muchmore.

impact your research

without devastating your bu dget

Proud Past; Dynamic Future

uoro SENS for the demanding researcher ... ... or the demands of routine measurements

Please contact us

Sales

[email protected]

+44 (0)141 952 9475

Technical [email protected]

+44 (0)141 952 9475
mailto:[email protected]:[email protected]:[email protected]:[email protected]:[email protected]:[email protected]:[email protected]:[email protected]:[email protected]


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fluoro SENS value-engineeredfor performanceWhen we designed this

remarkable single-photoncounting uorimeter we knewwhat we wanted to achieve a

specication to match the best research instrument on the market, for the price of a standard routine laboratory uorimeter.

Ambitious, yes, but we started from theground up with a clean sheet of paper and nopre-existing constraints just years ofpractical experience in designing,manufacturing and using uorimeters in real-life applications.

We thought through in detail each element ofthe design. Each component part had to befully optimised to meet our rigorous technicalspecication.

The result was uoro SENS , a single-photoncounting uorimeter which incorporates thelatest developments in optical, electronic andsoftware technology and it is highlycompetitively-priced too. There are no nastybudget surprises either, with many advancedfeatures provided as standard, including:

On-line real-time correction of excitationand emission spectra

Integrated automated lter-changer forinsertion of order-sorting lters as themeasurement proceeds

Flexible sample chamber with full sideaccess for easy interchange ofaccessories, such as integrating spheresfor quantum yield measurements, opticalbres for remote sensing, cryostats forultra-cold samples, 96-well plate reader,XY mapping stage and many more

Method les for recall of completeexperimental parameters

and many more time-saving features thatmake the system a completeluminescence measurement laboratory.

Your relationship with Gilden Photonics starts here

At Gilden Photonics we support you everystep of the way from selecting youruorimeter, to installing it in your laboratoryand then providing on-going support whenrequired.

Selecting the right instrument for yourapplication is often a difcult process. It

can be confusing comparing technicalspecications and trying to ensure thatmeasurements have been taken under thesame conditions. Meaningful comparisonscan often be hard to obtain because thereare no international standards for somespecications e.g. scan speed and waterRaman signal-to-noise.

Seeing is Believing

We are condent that we have designedour uoro SENS uorimeter to meet eventhe most demanding applications. Butdont just take our word for it put us to thetest!

You can send samples, visit us in ourlaboratories, request an on-linedemonstration or we can bring theuoro SENS to your laboratory anddemonstrate the instrument to you. In anymethod we can use your own samples andprovide you with your own personalisedmeasurement report.

Installation and support

All uoro SENS uorimeters are pre-alignedand fully calibrated before they leave ourfacility. We can undertake a completeinstallation at your laboratory and ensurethat you are totally satised with your newuoro SENS . Should you need any supportthereafter, whether it be application-development or service, our technical team

is always on hand to help via our dedicatedsupport channels.

About Gilden PhotonicsGilden Photonics Ltd. is a specialistspectroscopy company packed with real-world experience. Our staff bring over 75man-years of practical experience inspectroscopy instrumentation, design anddevelopment, manufacture and customersupport. All of our products are designed andcongured with the user in mind. Our goal isalways to provide our customers with rst-rateinstrumentation, supported by sound technicaladvice, at an affordable price.

Gilden Photonics Ltd. is based in Glasgow onthe state-of-the-art Clydebank Business Park,

just minutes from Glasgow Airport. Thecompany occupies purpose-built premiseswhich include a suite of ofces, manufacturingfacility and customer application, developmentand demonstration laboratories.


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The ideal uorimeter should be extremely sensitive, with no noisesignals. It should be capable of measuring true excitation and trueemission spectra without any interference in signals or non-uniformitiesin spectral output. The real world, however, is never idealbut withuoro SENS we have come pretty close!

Demonstrated below are some of the reasons that make uoro SENS such a high-performance and versatile laboratory companion.

Ultimate Single-Photon Counting Sensitivity

Single-photon counting is the ultimate when it comes to uorescencespectroscopy techniques. The signal is measured digitally, allowingany background noise to be eliminated. Samples can be measuredwith ease down to femto-molar concentrations.

The excitation and emission monochromators included in theuoro SENS have been designed to out-perform conventionalinstruments. The high-aperture and high-throughput optical designcollects and transfers more signal to the detector. At the same timethey offer excellent stray-light performance. This ensures that you willhave no problems with photo-bleaching.

uoro SENS incorporates the latest single-photon counting technologyto offer you a high-performance uorimeter with outstanding

sensitivity. The water Raman test is the standard frequently used tomeasure sensitivity. The plot below shows the performance foruoro SENS and the experimental conditions under which it wasobtained.

Sample: Raman Spectrum of Distilled WaterMeasurement Conditions: ! ex = 350nm, ! ! ex = ! ! em = 5nm,step size =1nm, integration time = 1sSignal to noise ratio > 3,000:1 for water Raman signal, ! peak = 397nm.Background noise measured at 450nm

Spectral Correction for Best Measurement Performance

In order to obtain true excitation and emission spectra it is necessaryto correct for any effects introduced by the instrument itself. Everyuoro SENS uorimeter gives you correction of both excitation andemission spectra included as standard a feature rarely found ininstruments in a comparable price bracket or even some of the so-called research grade uorimeters.

Sample: Pyrene in cyclohexane (2x10 -3M)Measurement Conditions: ! ex = 338nm, ! ! ex = ! ! em = 0.5nm,step size = 0.5nm, integration time = 1s

Corrected excitation spectra

uoro SENS provides you with on-line, real-time corrected excitationspectra. This is achieved by incorporating a large-area, high-stabilityreference photodiode which has been calibrated to give the truecorrection of excitation intensity at every measurement point.

Corrected emission spectraIncluded with every uoro SENS is a factory-determined correctionfunction which has been derived using a National Standards Accreditedtungsten calibration lamp This guarantees the measurement of trueemission spectra from your samples every time.

The ideal uorimeter:uoro SENS


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Comprehensive is STANDARD

Excellent Spectral Resolution for Fine Line Structures

The high-resolution monochromators in the uoroSENS permit spectralresolution from 0.1nm. This allows ne line structures to be resolvedwith outstanding accuracy and repeatability. The graphs below show 10repeat scans with close up of the spectra peaks demonstrating theexcellent spectral resolution of the uoroSENS.

High Dynamic Range

The exceptionally high dynamic range of uoro SENS allows you toview weak and strong uorescence peaks simultaneously from the one

scan without any time-consuming changes in conguration orinstrument saturation. No longer do you need to make severalmeasurements under different conditions to resolve strong and weaksignals.

Transmission Detector for Checking Sample Stability

The optional transmission detector allows the direct monitoring of thetransmission intensity through your sample. This is achieved throughsimultaneous acquisition of sample data during scans.

You can be condent of sample integrity during measurement. Thisfeature is particularly ideal for life-sciences applications, providing areal-time, on-line check of your sample for photo-bleaching, cell death,sample settling etc.

All measurement modes can utilise this detector option including thedemands of anisotropy analysis.

Broad Spectral Range from VUV to NIR

There is a wide range of single-photon counting detectors available foruse with uoro SENS which can cover from the UV to NIR. Choice ofdetector depends on application requirements. The most popular areblue- or red-sensitivity single-photon counting photomultipliers withspectral ranges of 185-670nm or 185-870nm respectively. Otherdetectors are available upon request.

Method Files for Complete Recall of Experimental Parameters

uoro SENS will retain all your experimental details with a single methodbutton, providing you with an instant record of all previous parametersused. In addition to the actual data, all method and report information,calculations and graphical outputs can be displayed. This feature isparticularly useful when you wish to re-run experiments under preciselythe same conditions. It also means that each user in your laboratorycan efciently set-up and store their particular measurement methodsand experiments.

Compact Footprint and USB 2.0 Connectivity

uoro SENS is a highly compact desktop instrument occupying afraction of the space of most research instruments. Measuring just0.6m (D) x 0.83m (W), it will t conveniently on the laboratory bench.The low and at prole of uoro SENS makes it ideal to place either alaptop computer or monitor and keyboard on top of the instrument andstill maintain a comfortable working height, thus saving further demandson laboratory space.

Each uoro SENS has a security locking point for computer hardware.

No special computer interface cards are needed, simply connect usinga USB 2.0 cable.


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Analysing and Manipulating Data

ANALYSIS OF SPECTRA Flexible 2D, 3D and Contour Charting Spectral Correction (during or post acquisition)

Spectral Arithmetic (+, -, x, /) Smoothing

Normalisation Zooming

1st, 2nd, 3rd Derivatives Anisotropy Analysis including G-factor Correction

Integration Quantum Yield Analysis

Differentiation Peak Search

CONTROL FEATURESWavelength Selection ( ! ex and ! em ) Spectral Scan Limits

Integration Time per Data Point Bandpass Selection (ex and em)

Sample Selection (4 position) Polariser Selection (Pre-Set Angle or ManualSelection)

Live Signal Count for Sample PositioningOptimisation

Repeat Scan Control

Plate Reader Control Real-time Spectral Data Correction

Post-Acquisition Spectral Correction Signal Attenuator


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Key uoro SENS features:Excitation and EmissionMonochromators

300mm focal length, Czerny Turner f/4Standard Gratings, 300nm blaze (Exc),500nm blaze (Em)Motorised triple-grating turret offeringcapability for UV to NIR optimised coverageContinuously-variable motorized bilateralknife-edge slitsSpectral dispersion 2.7nm/mm, slit widths10 microns to 3mmWavelength accuracy 0.1nm; wavelengthreproducibility 0.1nm

All specications standard with1200g/mm gratings

Light Source150W xenon arc lampOptical intensity stability better than 0.1%F/1 collection, fused silica opticsEasy interchange of bulb on siteIntegrated power supplyPre-aligned mountUltra-stable excitation source for precisionsteady-state measurements

Auto-ignition of lamp on instrument switch-on

Sample ChamberHolder for liquid samples on precisionrotation stageFull side access for easy interchange ofaccessoriesDetector protection interlock on samplechamber lidF/1 optics for excitation and emissionchannelsSpacious, exible sample chamber to mountvarious cryostatsOptions for solid, powder and lm samples;XYZ position adjustment; water-thermostattable cell holder with magneticstirrer

Automated Filter Changer

Auto-insertion of order-sorting lter asmeasurement proceedsUp to 5 selectable ltersFilter selection automatically included in thespectral correction functionReduced stray and scattered light

DetectorStandard single-photon countingphotomultiplier (185-650nm)Optional extended range, low noisephotomultiplier (185-870nm)Choice of blue- or red- sensitive PMT, NIRdetector, InGaAs photodiode

ElectronicsHigh-speed digital signal processing forhardware control and data acquisitionSingle USB 2.0 connection to host computer no interface cards needed for PC90-260V ac, 50/60HzUser-control interface

Other standard featuresBeam splitter and reference siliconphotodiode for automatic correction ofexcitation spectraStored emission channel transmission forautomatic correction of emission spectraOptical signal attenuator up to 100:1Computer security mounting point

Gratings option for up to 3 different blazegratings to be tted within both excitation andemission monochromators for highestpossible optical throughput and wide spectralrange


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... and accessories! -BBO Glan Thomson motorized polariser for the study ofsteady-state uorescence anisotropy and other polarisation effects akey measurement in life sciences, drug interaction studies, photo-chemistry and polymer studies.

1 resolution for anisotropy measurementsSpectral range 200nm 3500nmSuperb extinction coefcient 5 x 10 -6 guaranteeing exceptionalpolarisation purityMotorised positioning into the beamMotorised angular selection 0, 90, magic angle and free selection

Solid, powder and lm sample holders which are easilyinterchangeable to suit all types of sample

Water-thermostattable cell holder with magnetic stirrer keepsyour sample at a constant temperature. Supplied with a stirrer formixing viscose liquids while taking measurements

Automated four-position cell holder allows you to take multiplesample measurements without having to re-congure your experimenteach time

Front-face cell holder with rotation a sample holder with frontface excitation designed for use with thin lms, microscope slides,bres and powders. Fitted with a rotation facility for angle adjustment

96-Well plate reader ( used with the bre optic accessory) allowsyou to scan large numbers of samples rapidly


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A complete uoro SENS system

ready for your applicationuoroSENS - QY, QUANTUM YIELD SYSTEMfor high performance Quantum Yield measurements

The measurement of Quantum Yields (QY) is a fundamental property ofa luminescent sample. It refers specically to the ratio of the number ofphotons of light radiated from the photoluminescent material to thenumber of photons of light that the material absorbs.

Practically, QYs are very important because they allow the assessmentof uorometric materials and the extent of interferences. As such thesemeasurements are made on solutions, powders and thin lms and applyto a wide range of applications including, for example, uorescentmaterials for whiteners and white lights, organic and inorganic LEDmaterials, biology, uorescent probes and quantum dots, laserthreshold requirements, determining suitability of wavelength shiftersand for studies of radiation-less transitions in molecular systems.

The uoro SENS -QY achieves high performance through the use of anintegrating sphere. The reason for this is that by the time the lightreaches the detector much of the original emission anisotropy has beeneliminated so that the detector gives a good measure of the totalemitted radiant power. In addition there is the benet that there is arelative lack of sensitivity to scattering and the geometry of the sample.

However, the sphere walls reect the excitation light, as well as theemission light, which can be re-absorbed by the sample. This re-absorption is quite important for the excitation peak because part ofthis light, either reected from or transmitted through the sample, canbe re-absorbed by the sample after reection from the sphere wall.

uoroSENS - PR, PLATE READER SYSTEM

enabling high sample throughput withoutcompromising on sensitivity and resolution

For many analysis methods the use of 96 and 384 well plates havebecome the dened standard method for medium and higherthroughput assays. uoro SENS -PR has a uorimeter packagespecically designed to exploit the uoro SENS measurement, powerand resistivity combined with a Plate Reader system. The large samplearea easily permits insertion and removal of plates. This instrument isideal for many applications where large numbers of samples need to bemeasured using a repeatable method and often nds applications in bioand chemi-analysis. Complete plates, stacked volumes and/or rows oreven individual wells can be measured.

uoroSENS-HS HIGH SPEED SCANNING SYSTEM

for ultra-fast scanning of your samples

Speed of scan can be important where the sample will degrade quickly.It can also be an important factor for gathering quick measurementswithout the requirement for high resolution.

The uoro SENS -HS single photon counting sensitivity and proprietaryhigh speed scanning capability provides a perfect combination.

uoroSENS-P, PHOSPHORESCENCE SYSTEM

making your Phosphorescence measurementsconvenient and accurate

uoro SENS -P is specically-designed for time-resolved and steady-state Phosphorescence measurements. This uoro SENS version uses apulsed xenon lamp or an optional laser to provide the excitations and

time-gated detection to separate the high-intensity uorescence signalfrom the phosphorescence.

The standard uoro SENS system comes with a high-speed shutter inthe excitation channel that permits phosphorescence measurements ofmedium lifetime. The uoro SENS P system improves ne resolutionusing a pulsed Xe lamp for lifetimes down to a few 10s microseconds.Fast photon counting is standard at time-resolutions from 1 " s per point.

uoroSENS-PL, PHOTOLUMINESCENCE MAPPINGSYSTEM

your PL mapping tool for quality control and materials

investigationPhotoluminescence mapping from semiconductor wafers has becomean important QA tool for the assessment of alloy concentration and thequality of wafers and devices and devices quality.

The uoro SENS -PL instrument comprises a high sensitivity, rapidscanning system for wafers up to 4 diameter.

The method software allows mapping in rows, columns or other user-dened patterns across the wafer, permitting the user to customise themap requirements.

uoroSENS-EL, ELECTRO-LUMINESCENCE SYSTEM

complete system for measuring yourElectroluminscence samples

The uoro SENS -EL is a unique instrument for the measurement ofElectroluminescence samples. Complete with the EL driver system,LEDs and other EL materials can be driven under precisely-controlledcurrent and voltage conditions to determine their I-V and spectralemission characteristics. Output spectra can be readily connected toshow co-ordinates for light colour assessments.


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uoro SENS key specicationsPARAMETER SPECIFICATIONOptical conguration Right-angle geometry; optical accessories for other geometries

Excitation source 150W continuous Xenon arc lamp, ozone freeOptical intensity stability better than 0.1%200-2600nm spectral rangeXYZ bulb, rear mirror and lamp focus adjustment factory pre-alignedUser interchangeable xenon bulb

Monochromators Czerny-Turner design, 300mm focal length

Triple-grating turret as standardMotorized continuously-variable precision knife-edge slits from 10 to 3mm asstandardStray light performance 1:10 5

Excitation monochromator 1200g/mm, 300nm blaze gratingEmission monochromator 1200g/mm, 500nm blaze gratingWavelength accuracy 0.2nmWavelength repeatability 0.1nmBandpass 0.1 10nm continuously variable in steps of 0.05nmScan speed 80nm/s depends upon grating

Detector 185-670nm, blue-sensitivity single-photon counting photomultiplier; dark count rate


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uoro SENS -THE IDEAL FLUORIMETER

Sensitivity Single photon detection,Guaranteed Water Raman signal-to-noise >= 3,000:1

Dynamic Range100MHz photon counting system and one of the loweststray light and dark noise performance levels.

Spectral ResolutionResolution from 0.05nm FWHM.Exceptional monochromator turret reproducibility.High speed scanning facility.

Fully automated slits.

Spectral CorrectionOn-line, real-time lamp monitoring and spectralcorrection of the excitation channel.Emission channel correction using calibrated lamps.

SoftwareMethod les for experiment set-up.Comprehensive measurement protocols.Data analysis and presentation graphics.

Size & PriceSize matters - compact desktop system withoutmeasurement compromise.USB 2.0 connectivity-no need for a dedicated, costlyinterface card.Matched wi th an equally compact pr ice.

Gilden Photonics Ltd.,Unit 13-14 Telford Court,9 South Avenue,Clydebank Business Park,Clydebank, G81 2NR, UK

Tel: +44-(0)14 1-952-9475Fax: +44-(0)14 1-952-9400Eml: [email protected]: www.gildenphotonics.com

Distributor :

Gilden Photonics Ltd has a policy of continuous product development and reserves the right to amend specications without prior notice. E & OE.

Seeing is believing ...

Contact us for your own sample measurement report.

Visit our facility, your local distributor or watch on-line as your samples are being measuredand discuss the results with our application scientists.
mailto:[email protected]://www.gildenphotonics.com/mailto:[email protected]://www.gildenphotonics.com/mailto:[email protected]://www.gildenphotonics.com/http://www.gildenphotonics.com/mailto:[email protected]:[email protected]

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FluoroSENS - Fluorimeter